Improved Method for the Microestimation of Paper Chromatographied Substance

For the micro-estimation of substances in the spots on paper chromatogram, I have extr acted the solute by means of the improved apparatus (Fig. 1, 2. ). A cut spot of paper chromatogram (P) is inserted in the glass tube, in which there is a paper bridge (B) in order to regulate the rate of extraction, and is washed with the suitable solvent (S). I have estimated the flavonoide pigments by this apparatus.