105 results on '"L. Notarangelo"'
Search Results
2. Inborn errors of type I IFN immunity in patients with life-threatening COVID-19
- Author
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Universitat Rovira i Virgili, Zhang Q, Bastard P, Liu Z, Le Pen J, Moncada-Velez M, Chen J, Ogishi M, Sabli IKD, Hodeib S, Korol C, Rosain J, Bilguvar K, Ye J, Bolze A, Bigio B, Yang R, Arias AA, Zhou Q, Zhang Y, Onodi F, Korniotis S, Karpf L, Philippot Q, Chbihi M, Bonnet-Madin L, Dorgham K, Smith N, Schneider WM, Razooky BS, Hoffmann HH, Michailidis E, Moens L, Han JE, Lorenzo L, Bizien L, Meade P, Neehus AL, Ugurbil AC, Corneau A, Kerner G, Zhang P, Rapaport F, Seeleuthner Y, Manry J, Masson C, Schmitt Y, Schlüter A, Le Voyer T, Khan T, Li J, Fellay J, Roussel L, Shahrooei M, Alosaimi MF, Mansouri D, Al-Saud H, Al-Mulla F, Almourfi F, Al-Muhsen SZ, Alsohime F, Al Turki S, Hasanato R, van de Beek D, Biondi A, Bettini LR, D'Angio M, Bonfanti P, Imberti L, Sottini A, Paghera S, Quiros-Roldan E, Rossi C, Oler AJ, Tompkins MF, Alba C, Vandernoot I, Goffard JC, Smits G, Migeotte I, Haerynck F, Soler-Palacin P, Martin-Nalda A, Colobran R, Morange PE, Keles S, Çölkesen F, Ozcelik T, Yasar KK, Senoglu S, Karabela ?N, Gallego CR, Novelli G, Hraiech S, Tandjaoui-Lambiotte Y, Duval X, Laouénan C,, Snow AL, Dalgard CL, Milner J, Vinh DC, Mogensen TH, Marr N, Spaan AN, Boisson B, Boisson-Dupuis S, Bustamante J, Puel A, Ciancanelli M, Meyts I, Maniatis T, Soumelis V, Amara A, Nussenzweig M, García-Sastre A, Krammer F, Pujol A, Duffy D, Lifton R, Zhang SY, Gorochov G, Béziat V, Jouanguy E, Sancho-Shimizu V, Rice CM, Abel L, Notarangelo LD, Cobat A, Su HC, Casanova JL COVID-STORM Clinicians, COVID Clinicians, Imagine COVID Group, French COVID Cohort Study Group, CoV-Contact Cohort, Amsterdam UMC Covid-19, Biobank, COVID Human Genetic Effort, NIAID-USUHS, TAGC COVID Immunity Group, Universitat Rovira i Virgili, and Zhang Q, Bastard P, Liu Z, Le Pen J, Moncada-Velez M, Chen J, Ogishi M, Sabli IKD, Hodeib S, Korol C, Rosain J, Bilguvar K, Ye J, Bolze A, Bigio B, Yang R, Arias AA, Zhou Q, Zhang Y, Onodi F, Korniotis S, Karpf L, Philippot Q, Chbihi M, Bonnet-Madin L, Dorgham K, Smith N, Schneider WM, Razooky BS, Hoffmann HH, Michailidis E, Moens L, Han JE, Lorenzo L, Bizien L, Meade P, Neehus AL, Ugurbil AC, Corneau A, Kerner G, Zhang P, Rapaport F, Seeleuthner Y, Manry J, Masson C, Schmitt Y, Schlüter A, Le Voyer T, Khan T, Li J, Fellay J, Roussel L, Shahrooei M, Alosaimi MF, Mansouri D, Al-Saud H, Al-Mulla F, Almourfi F, Al-Muhsen SZ, Alsohime F, Al Turki S, Hasanato R, van de Beek D, Biondi A, Bettini LR, D'Angio M, Bonfanti P, Imberti L, Sottini A, Paghera S, Quiros-Roldan E, Rossi C, Oler AJ, Tompkins MF, Alba C, Vandernoot I, Goffard JC, Smits G, Migeotte I, Haerynck F, Soler-Palacin P, Martin-Nalda A, Colobran R, Morange PE, Keles S, Çölkesen F, Ozcelik T, Yasar KK, Senoglu S, Karabela ?N, Gallego CR, Novelli G, Hraiech S, Tandjaoui-Lambiotte Y, Duval X, Laouénan C,, Snow AL, Dalgard CL, Milner J, Vinh DC, Mogensen TH, Marr N, Spaan AN, Boisson B, Boisson-Dupuis S, Bustamante J, Puel A, Ciancanelli M, Meyts I, Maniatis T, Soumelis V, Amara A, Nussenzweig M, García-Sastre A, Krammer F, Pujol A, Duffy D, Lifton R, Zhang SY, Gorochov G, Béziat V, Jouanguy E, Sancho-Shimizu V, Rice CM, Abel L, Notarangelo LD, Cobat A, Su HC, Casanova JL COVID-STORM Clinicians, COVID Clinicians, Imagine COVID Group, French COVID Cohort Study Group, CoV-Contact Cohort, Amsterdam UMC Covid-19, Biobank, COVID Human Genetic Effort, NIAID-USUHS, TAGC COVID Immunity Group
- Abstract
Clinical outcome upon infection with SARS-CoV-2 ranges from silent infection to lethal COVID-19. We have found an enrichment in rare variants predicted to be loss-of-function (LOF) at the 13 human loci known to govern TLR3- and IRF7-dependent type I interferon (IFN) immunity to influenza virus, in 659 patients with life-threatening COVID-19 pneumonia, relative to 534 subjects with asymptomatic or benign infection. By testing these and other rare variants at these 13 loci, we experimentally define LOF variants in 23 patients (3.5%), aged 17 to 77 years, underlying autosomal recessive or dominant deficiencies. We show that human fibroblasts with mutations affecting this pathway are vulnerable to SARS-CoV-2. Inborn errors of TLR3- and IRF7-dependent type I IFN immunity can underlie life-threatening COVID-19 pneumonia in patients with no prior severe infection.Copyright © 2020, American Association for the Advancement of Science.
- Published
- 2020
3. Reproductive strategy in women with critical ovarian reserve
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E. Porcu, G. Damiano, L. Cipriani, S. Rossi, F. Fabbri, F. Sacilotto, G. M. Cillo, L. Notarangelo, P. M. Ciotti, N. Calza, L. Vaccarella, and E. Porcu, G. Damiano, L. Cipriani, S. Rossi, F. Fabbri, F. Sacilotto, G.M. Cillo, L. Notarangelo, P.M. Ciotti, N. Calza, L. Vaccarella
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POI, Ovary Reserve, Infertility - Published
- 2018
4. Effects of cancer on ovarian reserve and ovarian response to stimulation
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E. Porcu, G. Damiano, L. Cipriani, S. Rossi, F. Fabbri, F. Sacilotto, G. M. Cillo, L. Notarangelo, P. M. Ciotti, N. Calza, L. Vaccarella, and E. Porcu, G. Damiano, L. Cipriani, S. Rossi, F. Fabbri, F. Sacilotto, G.M. Cillo, L. Notarangelo, P.M. Ciotti, N. Calza, L. Vaccarella
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Infertility, Cancer, Ovarian Reserve - Published
- 2018
5. Treatment of Traumatic Brain Injury–Induced Dyskinesia With Tetrabenazine: A Case Report
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Paula L. Notarangelo, Kristy S. Lane, Margo D. Lauterbach, and Vassilis E. Koliatsos
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Adult ,medicine.medical_specialty ,Dyskinesias ,Adrenergic Uptake Inhibitors ,Traumatic brain injury ,business.industry ,Tetrabenazine ,Accidents, Traffic ,medicine.disease ,Neuropsychiatry ,Psychiatry and Mental health ,Arts and Humanities (miscellaneous) ,Dyskinesia ,Brain Injuries ,medicine ,Humans ,Female ,medicine.symptom ,business ,Psychiatry ,Applied Psychology ,medicine.drug - Abstract
Received July 1, 2014; revised August 26, 2014; accepted August 26, 2014. From The Neuropsychiatry Program at Sheppard Pratt, Sheppard Pratt Health System, Baltimore, MD. Send correspondence and reprint requests to Margo Lauterbach, M.D., The Neuropsychiatry Program at Sheppard Pratt, Sheppard Pratt Health System, 6501 N. Charles Street, P.O. Box 6815, Baltimore, MD 21285-6815; e-mail: mlauterbach@sheppardpratt.org & 2015TheAcademy of PsychosomaticMedicine. Published by Elsevier Inc. All rights reserved. Introduction
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- 2015
6. Diagnostic and treatment challenges in traumatic brain injury patients with severe neuropsychiatric symptoms: insights into psychiatric practice
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Margo D. Lauterbach, Vassilis E Koliatsos, Stephen Nichols, Kristy S. Lane, and Paula L. Notarangelo
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medicine.medical_specialty ,Neuropsychiatric Disease and Treatment ,Referral ,treatment ,Traumatic brain injury ,business.industry ,traumatic brain injury ,neurobehavioral ,Psychological intervention ,Cognition ,Retrospective cohort study ,medicine.disease ,Neuropsychiatry ,Mood ,Cohort ,medicine ,business ,Psychiatry ,Original Research - Abstract
Margo D Lauterbach,1 Paula L Notarangelo,1 Stephen J Nichols,2 Kristy S Lane,1 Vassilis E Koliatsos11The Neuropsychiatry Program at Sheppard Pratt, Sheppard Pratt Health System, Baltimore, MD, 2Department of Emergency Medicine, The University of Tennessee College of Medicine Chattanooga, Chattanooga, TN, USAAbstract: Traumatic brain injury (TBI) causes a variety of neuropsychiatric problems that pose diagnostic and treatment challenges for providers. In this report, we share our experience as a referral neuropsychiatry program to assist the general psychiatrist when adult TBI patients with psychiatric symptoms present for evaluation and treatment. We completed a retrospective study of patients with moderate-to-severe TBI and severe neuropsychiatric impairments. We collected information on demographics, nature of injury, symptomatology, diagnoses, and treatments. Data analysis indicates that mood stabilization was a key concern, often requiring aggressive pharmacological management. Cognitive dysfunction was a problem for the majority of patients, but was only medicated in a third, due to poor efficacy or behavioral side effects. The co-occurrence of multiple TBI-related symptoms and diagnoses in this patient cohort emphasizes the need for individualized psychopharmacological approaches and interventions.Keywords: traumatic brain injury, neurobehavioral, treatment
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- 2015
7. SAP and Lessons Learned from a Primary Immunodeficiency
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J, Sayos, C, Wu, M, Morra, N, Wang, X, Zhang, D, Allen, S, van Schaik, L, Notarangelo, R, Geha, M G, Roncarolo, H, Oettgen, J E, De Vries, G, Aversa, and C, Terhorst
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0301 basic medicine ,Adult ,Male ,T-Lymphocytes ,Immunology ,Biology ,Lymphocyte Activation ,Article ,03 medical and health sciences ,Jurkat Cells ,Mice ,Young Adult ,Signaling Lymphocytic Activation Molecule Family Member 1 ,Cell surface receptor ,Allergy and Immunology ,medicine ,Immunology and Allergy ,Animals ,Humans ,Signaling Lymphocytic Activation Molecule Associated Protein ,Cloning, Molecular ,Gene ,SIGNALING LYMPHOCYTE ACTIVATION MOLECULE ,Cloning ,Immunologic Deficiency Syndromes ,Signal transducing adaptor protein ,History, 20th Century ,medicine.disease ,Lymphoproliferative Disorders ,Cell biology ,Mice, Inbred C57BL ,030104 developmental biology ,Mutation ,Primary immunodeficiency ,Sequence Alignment ,Intracellular - Abstract
Approximately 19 years ago, Terhorst and colleagues ([1][1]) reported the cloning of the gene encoding a small adaptor protein, signaling lymphocyte activation molecule (SLAM)-associated protein (SAP), named by virtue of its binding to the intracellular tail of the cell surface receptor SLAM.
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- 2017
8. EFFECTS OF WATER REGIMES ON VINE PERFORMANCE AND QUALITY OF 'SUBLIMA' SEEDLESS TABLE GRAPE COVERED WITH PLASTIC FILM TO ADVANCE GRAPE RIPENING
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A.M. Amendolagine, G. Vox, D. Di Gennaro, Evelia Schettini, L. Notarangelo, L. de Palma, and L. Tarricone
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Horticulture ,Vine ,Table grape ,Plastic film ,Environmental science ,Ripening - Published
- 2014
9. FIRST DELIVERY FROM AUTOLOGOUS FROZEN EGGS IN A CANCER PATIENT
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PORCU, ELEONORA, VENTUROLI, STEFANO, DAMIANO, GIUSEPPE, P. M. CIOTTI, L. NOTARANGELO, PARADISI, ROBERTO, M. MOSCARINI, G. AMBROSINI, E. PORCU, S. VENTUROLI, G. DAMIANO, P.M. CIOTTI, L. NOTARANGELO, R. PARADISI, M. MOSCARINI, and G. AMBROSINI
- Abstract
ANTINEOPLASTICTREATMENT HAVE SIGNIFICANTLY INCRESED THE SURVIVAL OF CANCER PATIENTS WHO, HOWEVER, RISSK PREMATURE MENOPAUSE. OOCYTE CRYOPRESERVATION HAS RECENTLY BEEN PROPOSED AS A FERTILITY- SAVING OPTION. THIS REPORT DESCRIBES THE FIRST LIVE BIRTH ACHIEVED WITH AUTOLOGOUS CRYOPRESERVED OOCYTES IN AN OVARIECTOMIZATED BOREDERLINE CANCER PATIENT.
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- 2007
10. Defective Self-Reactive Antibody Repertoire of Serum IgM in Patients with Hyper-IgM Syndrome
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S, Lacroix-Desmazes, I, Resnick, D, Stahl, L, Mouthon, T, Espanol, J, Levy, S V, Kaveri, L, Notarangelo, M, Eibl, A, Fischer, H, Ochs, and M D, Kazatchkine
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Male ,Adolescent ,Stomach ,Immunology ,Syndrome ,Kidney ,Autoantigens ,Antigen-Antibody Reactions ,Immunoglobulin M ,Liver ,Child, Preschool ,Hypergammaglobulinemia ,Immunoglobulin G ,Pseudomonas aeruginosa ,Staphylococcus epidermidis ,Humans ,Immunology and Allergy ,Female ,Child ,Lung ,Autoantibodies - Abstract
We have analyzed the self-reactive repertoires of IgM and IgG Abs in the serum of 19 patients with hyper-IgM syndrome (HIM) by means of a quantitative immunoblotting technique that allows for a quantitative comparison of Ab repertoires in health and disease by multiparametric statistical analysis. Normal tissue extracts of liver, lung, stomach, and kidney were used as sources of self Ags. Extracts of Pseudomonas aeruginosa and Staphylococcus epidermidis were used as sources of nonself Ags. We demonstrate a significant bias in repertoires of reactivities of IgM of patients with HIM with self Ags. Ab repertoires of IgM toward nonself Ags did not differ, however, between patients and controls. No difference was found between IgM repertoires of untreated patients and those of patients receiving substitutive treatment with i.v. IgG. IgG in the serum of HIM patients lacked reactivity with self Ags, although it exhibited a pattern of reactivity with nonself Ags that was similar to that of IgG of healthy controls. The data demonstrate that functional CD40-CD40 ligand interactions are essential for the selection of natural self-reactive B cell repertoires.
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- 1999
11. Acute pancreatitis in the paediatric age group: a personal experience
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A, Cosentini, G, Stranieri, S, Capillo, L, Notarangelo, L, Madonna, S, Iannini, V, Ferro, V, Defilippo, R G, Defilippo, and R, Rubino
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Adult ,Male ,Adolescent ,Italy ,Pancreatitis ,Recurrence ,Humans ,Female ,Child ,Retrospective Studies - Abstract
Although relatively rare, acute pancreatitis is the most common disease complex involving the pancreas in the paediatric age group. The etiology of the disease is often unknown, and Italian epidemiological data on the paediatric population and, in particular, on the etiology of the disease are not available (except for studies of prevalence). Within the field of the most frequently encountered pancreatitis in the age range of our interest (i.e. 0-18 years), not only the commonly observed forms whose etiopathogenesis is ascribable to cholelithiasis must be mentioned but also those forms due to proteic-caloric malnutrition that are becoming increasingly common. The presenting clinical symptoms and signs may not be typical and the laboratory tests may not always be sensitive enough. In such age range chronic recurrent pancreatitis plays a very important epidemiologic role. Approximately 40% of children and teenagers admitted to the hospital with a diagnosis of pancreatitis report a previous episode of the disease. Irreversible changes in pancreatic parenchyma develop in those patients in whom the disease progresses, leading to pancreatic insufficiency. Such a morbid condition (chronic pancreatitis) is more often observed in adolescents, in whom the disease manifests itself with a vague repetitive dyspeptic symptomatology, after alternating remissions and recrudescences, not always clinically evident. In children, the clinical picture most commonly encountered is represented by recurrent abdominal pains, in view of the fact that the patients are frequently affected by thalassaemia. The pseudocystic evolution of the disease is the most common organic damage resulting from the chronic progression of the pancreatic impairment. A few differences have been found with respect to severity, etiology, and mortality of pancreatitis in the paediatric age group as compared with older age groups. Both the general practitioner with a paediatric practice and the paediatrician encounter a large number of difficulties in this field of pathology. Therefore, an adequate and correct "management" of children with acute or chronic pancreatitis seems to be mandatory.
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- 2005
12. X-linked lymphoproliferative disease: the dark side of 2b4 function
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C, Bottino, S, Parolini, R, Biassoni, M, Falco, L, Notarangelo, and A, Moretta
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Cytotoxicity, Immunologic ,Killer Cells, Natural ,Epstein-Barr Virus Infections ,Membrane Glycoproteins ,Antigens, CD ,Signaling Lymphocytic Activation Molecule Family ,Humans ,Receptors, Immunologic ,Lymphoproliferative Disorders ,Signal Transduction - Published
- 2002
13. [The value of the MR imaging in the evaluation of Müllerian duct anomalies]
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D, Console, S, Tamburrini, D, Barresi, L, Notarangelo, B, Bertucci, and O, Tamburrini
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Adult ,Adolescent ,Humans ,Female ,Magnetic Resonance Imaging ,Mullerian Ducts - Abstract
To reaffirm the role of MRI in the assessment of the M#159;llerian duct anomalies (MDAs).Between November 1997 and April 2001, 22 patients, age range 18-40 years (mean 29 years) were investigated with MRI and US. The MRI study was performed with a 0,5 Tesla imager (Vectra, GE Medical System) with body-coil; neither oral nor intravenous contrast was used. We obtained SE T1-weighted, fast STIR with fat suppression and FSE T2-weighted sequences. Sagittal, paracoronal and paraxial images were acquired. The paraxial images were obtained to produce true coronal images of the uterus. In evaluating MDAs, imaging the uterus in its true coronal plane is essential to assess the external fundal contour. US examination was performed with an HDI 3000, ATL, using the trans-vaginal approach. The MDAs were subdivided according to the Buttram and Gibbons classification.There were 22 cases of laparoscopic and hysteroscopic proved anomalies; MRI allowed correct diagnosis of 21 uterine anomalies (accuracy, 95%) whereas U.S. was correct in 20 of 22 cases (accuracy, 92%). The MRI was excellent in depicting the uterine morphology in one case of unicornuate uterus with rudimentary horn non-comunicating with the main cavity and distended by hematometra and associated hematosalpinx. Further-more evaluating composition, thickness and extension of the uterine septum and aspect of the fundal contour, MRI allowed to differentiate definitively between bicornuate uterus and septate uterus. This is a very important distinction to do because it significantly affects patient treatment: a septate uterus requires hysteroscopic septectomy, while a bicornuate uterus does not requires surgical treatment.Given its characteristics, MRI is a very accurate imaging modality in uterine evaluation and contributes significantly to treatment planning. Although ultrasonography remains the modality of choice for the initial study of patients who are suspected of having a MDAs, we propose, in accordance with many authors in the literature, to reserve MRI imaging for patients with a technically inadequate or indeterminate ultrasound examination.
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- 2001
14. Monocytes from Wiskott-Aldrich patients differentiate in functional mature dendritic cells with a defect in CD83 expression
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P, Allavena, R, Badolato, F, Facchetti, W, Vermi, C, Paganin, W, Luini, S, Giliani, C, Mazza, U, Bolzern, I, Chiesa, L, Notarangelo, A, Mantovani, and S, Sozzani
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Membrane Glycoproteins ,Antigens, CD ,Cell Movement ,T-Lymphocytes ,Antigen-Presenting Cells ,Humans ,Immunoglobulins ,B7-2 Antigen ,Dendritic Cells ,Lymphocyte Activation ,Endocytosis ,Monocytes ,Wiskott-Aldrich Syndrome - Abstract
Wiskott-Aldrich syndrome (WAS) is an X-linked disorder characterized by congenital thrombocytopenia and progressive deterioration of the immune function. Dendritic cells (DC) are key effectors in the induction of specific immunity and are highly specialized in antigen uptake and subsequent migration to draining lymph nodes. DC were generated in vitro from circulating monocytes from ten WAS patients characterized by a different disease score. Immature DC showed similar morphology and membrane phenotype, as compared to normal DC. In chemotaxis assay, immature DC had a reduced migration in response to MIP-1alpha/CCL3, but efficiently endocytosed the macromolecules FITC-dextran and FITC-albumin. Upon terminal differentiation with LPS or CD40 ligand, the acquisition of a mature surface phenotype was variably achieved among WAS patients, with increased expression of CD80, CD86 and DC-LAMP. In contrast, the expression of CD83 was usually low. A defective up-regulation of CD83 was also observed in the lymph node from one WAS patient, whose DC stained positively for DC-LAMP. Mature DC from all the patients tested, but one, significantly migrated in vitro in response to MIP-3beta, a finding confirmed in vivo by the detection of HLA-DR/DC LAMP-positive cells in secondary lymphoid organs. When tested in MLR assays, both immature and mature WAS DC induced allogenic T cell proliferation in a manner comparable to control DC. Collectively these results suggest that, although many functional activities of WAS DC are essentially similar to normal DC, subtle and selective alterations of DC differentiation were also observed, with reduced migratory activity of immature DC and defective CD83 expression upon maturation.
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- 2001
15. Novel Presentation of Omenn Syndrome in Association with Aniridia
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O DELMONTE, W SHEEHAN, D MILLER, A ROBERTS, F BONILLA, M MORRA, S PAI, L NOTARANGELO, and H OETTGEN
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Immunology ,Immunology and Allergy - Published
- 2008
16. A Case of Ataxia-Telengiectasia with Persistent Massive Lymphoproliferation
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Eftichia Stiakaki, L Notarangelo, Maria Kalmanti, I. Bolonaki, A. Kambourakis, and D Schindler
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Ataxia ,business.industry ,Pediatrics, Perinatology and Child Health ,Immunology ,Medicine ,medicine.symptom ,business - Published
- 1999
17. Report of the ESID collaborative study on clinical features and molecular analysis in X-linked hyper-IgM syndrome
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L NOTARANGELO
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Immunology ,Molecular Biology - Published
- 1998
18. First polar body morphology before ICSI is not related to embryo quality or pregnancy rate.
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P.M. Ciotti, L. Notarangelo, A.M. Morselli-Labate, V. Felletti, E. Porcu, and S. Venturoli
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PREGNANCY , *OVUM , *HUMAN artificial insemination , *MORPHOLOGY - Abstract
BACKGROUND: The aim of this study was to analyse the relationship between the first polar body (1st PB) morphology and the fertilization rate, cleavage rate, embryo quality, pregnancy and implantation rate. METHODS: This was a retrospective study on 167 consecutive cycles undergoing assisted reproduction with ICSI. The 1st PB morphology was evaluated at the moment of ICSI in the 596 injected oocytes and it was coded as intact or fragmented. The fertilization rate, cleavage rate, embryo quality (three grades), pregnancy rate, implantation rate and the time elapsed between oocyte retrieval and ICSI were evaluated. The 1st PB morphology was checked twice (denudation and ICSI) in a random sample of 180 oocytes in order to verify the effect of the in vitro culture. RESULTS: No significant relationship was found between the 1st PB morphology and the fertilization rate (P=0.703), cleavage rate (P=0.055), embryo quality (P=0.673), pregnancy rate (P=0.201) and implantation rate (P=0.511). A significant positive relationship (P=0.006) was found between the frequency of the 1st PB fragmentation and the time elapsed between denudation and ICSI. The pregnancy rate was significantly higher (P=0.008) when oocytes were injected between 5 and 7 h after retrieval rather than earlier or later. CONCLUSIONS: Our data suggest that the embryo quality, pregnancy rate and implantation rate are not related to the 1st PB fragmentation. The time which elapses between the oocyte retrieval and ICSI should be maintained at ∼6 h in order to obtain optimal results. [ABSTRACT FROM AUTHOR]
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- 2004
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19. Documentation: designing forms that serve you
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J D, Kramer and P L, Notarangelo
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Nursing Records ,Humans ,Nursing Staff, Hospital - Published
- 1986
20. Documentation
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Joan D. Kramer and Paula L. Notarangelo
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World Wide Web ,Documentation ,Leadership and Management ,Computer science ,Nursing records - Published
- 1986
21. Associazionismo e identità. L’esperienza di un’associazione di migranti a Genova
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Jourdan L., Notarangelo C., and Jourdan L., Notarangelo C.
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migranti, associazionismo, stereotipi - Abstract
L'articolo propone alcune riflessioni antropologiche allo scopo di mettere in luce l’infondatezza degli atteggiamenti essenzializzanti e dei processi di categorizzazione e di stereotipizzazione particolarmente evidenti nei confronti di chi è di religione musulmana, a partire da una ricerca etnografica effettuata a Genova presso un’associazione di giovani adulti migrati dal Marocco.
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- 2018
22. Is fertility preservation suggested in breast cancer patients with BRCA mutations
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Patrizia Ciotti, Eleonora Porcu, Giuseppe Damiano, Leonardo Notarangelo, N. Calza, G. M. Cillo, F. Sacilotto, Linda Cipriani, and E. Porcu, L. Cipriani, G. Damiano, F. Sacilotto, G.M. Cillo, L. Notarangelo, P.M. Ciotti, N. Calza
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0301 basic medicine ,Oncology ,Infertility ,medicine.medical_specialty ,030219 obstetrics & reproductive medicine ,business.industry ,Obstetrics and Gynecology ,Breast Cancer, Fertility Preservation, Infertility ,medicine.disease ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Breast cancer ,Reproductive Medicine ,Internal medicine ,medicine ,Fertility preservation ,business - Published
- 2018
23. La crioconservazione degli ovociti mediante congelamento lento
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PORCU, ELEONORA, NOTARANGELO, LEONARDO, VENTUROLI, STEFANO, P.M. CIOTTI, G. DAMIANO, A. BIANCHI, F. ALBONETTI, S. ROSSI, E. PORCU, PM. CIOTTI, G. DAMIANO, L. NOTARANGELO, A. BIANCHI, F. ALBONETTI, S. ROSSI, and S. VENTUROLI
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congelamento lento ,ovociti umani - Published
- 2009
24. Human TMEFF1 is a restriction factor for herpes simplex virus in the brain.
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Chan YH, Liu Z, Bastard P, Khobrekar N, Hutchison KM, Yamazaki Y, Fan Q, Matuozzo D, Harschnitz O, Kerrouche N, Nakajima K, Amin P, Yatim A, Rinchai D, Chen J, Zhang P, Ciceri G, Chen J, Dobbs K, Belkaya S, Lee D, Gervais A, Aydın K, Kartal A, Hasek ML, Zhao S, Reino EG, Lee YS, Seeleuthner Y, Chaldebas M, Bailey R, Vanhulle C, Lorenzo L, Boucherit S, Rozenberg F, Marr N, Mogensen TH, Aubart M, Cobat A, Dulac O, Emiroglu M, Paludan SR, Abel L, Notarangelo L, Longnecker R, Smith G, Studer L, Casanova JL, and Zhang SY
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- Animals, Female, Humans, Male, Homozygote, Interferon Type I metabolism, Interferon Type I immunology, Nectins genetics, Nectins metabolism, Neurons cytology, Neurons metabolism, Neurons virology, Pluripotent Stem Cells cytology, Virus Replication, Child, Preschool, Young Adult, Pedigree, Brain cytology, Brain metabolism, Brain virology, Encephalitis, Herpes Simplex virology, Encephalitis, Herpes Simplex metabolism, Herpesvirus 1, Human pathogenicity, Herpesvirus 1, Human physiology, Membrane Proteins deficiency, Membrane Proteins genetics, Membrane Proteins metabolism, Virus Internalization
- Abstract
Most cases of herpes simplex virus 1 (HSV-1) encephalitis (HSE) remain unexplained
1,2 . Here, we report on two unrelated people who had HSE as children and are homozygous for rare deleterious variants of TMEFF1, which encodes a cell membrane protein that is preferentially expressed by brain cortical neurons. TMEFF1 interacts with the cell-surface HSV-1 receptor NECTIN-1, impairing HSV-1 glycoprotein D- and NECTIN-1-mediated fusion of the virus and the cell membrane, blocking viral entry. Genetic TMEFF1 deficiency allows HSV-1 to rapidly enter cortical neurons that are either patient specific or derived from CRISPR-Cas9-engineered human pluripotent stem cells, thereby enhancing HSV-1 translocation to the nucleus and subsequent replication. This cellular phenotype can be rescued by pretreatment with type I interferon (IFN) or the expression of exogenous wild-type TMEFF1. Moreover, ectopic expression of full-length TMEFF1 or its amino-terminal extracellular domain, but not its carboxy-terminal intracellular domain, impairs HSV-1 entry into NECTIN-1-expressing cells other than neurons, increasing their resistance to HSV-1 infection. Human TMEFF1 is therefore a host restriction factor for HSV-1 entry into cortical neurons. Its constitutively high abundance in cortical neurons protects these cells from HSV-1 infection, whereas inherited TMEFF1 deficiency renders them susceptible to this virus and can therefore underlie HSE., (© 2024. The Author(s).)- Published
- 2024
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25. COVID-19 Vaccination in Patients with Inborn Errors of Immunity Reduces Hospitalization and Critical Care Needs Related to COVID-19: a USIDNET Report.
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McDonnell J, Cousins K, Younger MEM, Lane A, Abolhassani H, Abraham RS, Al-Tamemi S, Aldave-Becerra JC, Al-Faris EH, Alfaro-Murillo A, AlKhater SA, Alsaati N, Doss AMA, Anderson M, Angarola E, Ariue B, Arnold DE, Assa'ad AH, Aytekin C, Bank M, Bergerson JRE, Bleesing J, Boesing J, Bouso C, Brodszki N, Cabanillas D, Cady C, Callahan MA, Caorsi R, Carbone J, Carrabba M, Castagnoli R, Catanzaro JR, Chan S, Chandra S, Chapdelaine H, Chavoshzadeh Z, Chong HJ, Connors L, Consonni F, Correa-Jimenez O, Cunningham-Rundles C, D'Astous-Gauthier K, Delmonte OM, Demirdag YY, Deshpande DR, Diaz-Cabrera NM, Dimitriades VR, El-Owaidy R, ElGhazali G, Al-Hammadi S, Fabio G, Faure AS, Feng J, Fernandez JM, Fill L, Franco GR, Frenck RW, Fuleihan RL, Giardino G, Galant-Swafford J, Gambineri E, Garabedian EK, Geerlinks AV, Goudouris E, Grecco O, Pan-Hammarström Q, Khani HHK, Hammarström L, Hartog NL, Heimall J, Hernandez-Molina G, Horner CC, Hostoffer RW, Hristova N, Hsiao KC, Ivankovich-Escoto G, Jaber F, Jalil M, Jamee M, Jean T, Jeong S, Jhaveri D, Jordan MB, Joshi AY, Kalkat A, Kanarek HJ, Kellner ES, Khojah A, Khoury R, Kokron CM, Kumar A, Lecerf K, Lehman HK, Leiding JW, Lesmana H, Lim XR, Lopes JP, López AL, Tarquini L, Lundgren IS, Magnusson J, Marinho AKBB, Marseglia GL, Martone GM, Mechtler AG, Mendonca L, Milner JD, Mustillo PJ, Naderi AG, Naviglio S, Nell J, Niebur HB, Notarangelo L, Oleastro M, Ortega-López MC, Patel NR, Petrovic G, Pignata C, Porras O, Prince BT, Puck JM, Qamar N, Rabusin M, Raje N, Regairaz L, Risma KA, Ristagno EH, Routes J, Roxo-Junior P, Salemi N, Scalchunes C, Schuval SJ, Seneviratne SL, Shankar A, Sherkat R, Shin JJ, Siddiqi A, Signa S, Sobh A, Lima FMS, Stenehjem KK, Tam JS, Tang M, Barros MT, Verbsky J, Vergadi E, Voelker DH, Volpi S, Wall LA, Wang C, Williams KW, Wu EY, Wu SS, Zhou JJ, Cook A, Sullivan KE, and Marsh R
- Subjects
- Humans, COVID-19 Vaccines adverse effects, Vaccination, Hospitalization, Critical Care, COVID-19 epidemiology
- Abstract
Background: The CDC and ACIP recommend COVID-19 vaccination for patients with inborn errors of immunity (IEI). Not much is known about vaccine safety in IEI, and whether vaccination attenuates infection severity in IEI., Objective: To estimate COVID-19 vaccination safety and examine effect on outcomes in patients with IEI., Methods: We built a secure registry database in conjunction with the US Immunodeficiency Network to examine vaccination frequency and indicators of safety and effectiveness in IEI patients. The registry opened on January 1, 2022, and closed on August 19, 2022., Results: Physicians entered data on 1245 patients from 24 countries. The most common diagnoses were antibody deficiencies (63.7%). At least one COVID-19 vaccine was administered to 806 patients (64.7%), and 216 patients received vaccination prior to the development of COVID-19. The most common vaccines administered were mRNA-based (84.0%). Seventeen patients were reported to seek outpatient clinic or emergency room care for a vaccine-related complication, and one patient was hospitalized for symptomatic anemia. Eight hundred twenty-three patients (66.1%) experienced COVID-19 infection. Of these, 156 patients required hospitalization (19.0%), 47 required ICU care (5.7%), and 28 died (3.4%). Rates of hospitalization (9.3% versus 24.4%, p < 0.001), ICU admission (2.8% versus 7.6%, p = 0.013), and death (2.3% versus 4.3%, p = 0.202) in patients who had COVID-19 were lower in patients who received vaccination prior to infection. In adjusted logistic regression analysis, not having at least one COVID-19 vaccine significantly increased the odds of hospitalization and ICU admission., Conclusion: Vaccination for COVID-19 in the IEI population appears safe and attenuates COVID-19 severity., (© 2024. The Author(s).)
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- 2024
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26. Lack of Evidence for a Role of ACE-2 Polymorphisms as a Bedside Clinical Prognostic Marker of COVID-19.
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Fiore JR, Di Stefano M, Oler A, Zhang Y, Gu J, Dalgard CL, Faleo G, Epling B, Notarangelo L, Lisco A, and Santantonio TA
- Subjects
- Humans, Angiotensin-Converting Enzyme 2 genetics, Biomarkers, Polymorphism, Genetic, Prognosis, COVID-19 diagnosis, COVID-19 genetics, SARS-CoV-2 genetics
- Abstract
The novel SARS-CoV-2 coronavirus causes a severe respiratory syndrome referred to as coronavirus disease (COVID-19). The angiotensin-converting enzyme 2 (ACE-2) plays an important role as a cellular receptor for SARS-CoV-2 and is largely expressed in lungs, kidneys, heart and the gastrointestinal tract along with being shed in plasma. The ACE-2 gene and protein show a high level of genetic polymorphism, including simple nucleotide variation, transcriptional variation, post-transcriptional changes, and putative protein mutations that could interfere with the binding or entry of SARS-CoV-2 and affect tissue damage in lungs or other organs. Genetic polymorphisms can impact SARS-CoV-2 viral entry and COVID-19 severity. This single-center study evaluated the possible role of the main ACE-2 polymorphisms (rs143936283, rs2285666, rs41303171, rs35803318, and rs2106809) as potential prognostic markers in SARS-CoV-2-infected individuals. Frozen whole blood was used for DNA isolation and genomic DNA samples were sheared using the Covaris LE220 Focused-ultrasonicator for targeting a peak size of 410 bp. Whole-genome sequencing libraries were generated from fragmented DNA using the Illumina TruSeq DNA PCR-Free HT Library Preparation Kit and sequenced on an Illumina NovaSeq 6000. We did not identify any correlation between ACE-2 polymorphisms and COVID-19 prognosis, suggesting that the interpretation and clinical use of ACE-2 genetic polymorphisms in real-world clinical settings requires further experimental and clinical validation.
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- 2023
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27. Inborn errors of OAS-RNase L in SARS-CoV-2-related multisystem inflammatory syndrome in children.
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Lee D, Le Pen J, Yatim A, Dong B, Aquino Y, Ogishi M, Pescarmona R, Talouarn E, Rinchai D, Zhang P, Perret M, Liu Z, Jordan I, Elmas Bozdemir S, Bayhan GI, Beaufils C, Bizien L, Bisiaux A, Lei W, Hasan M, Chen J, Gaughan C, Asthana A, Libri V, Luna JM, Jaffré F, Hoffmann HH, Michailidis E, Moreews M, Seeleuthner Y, Bilguvar K, Mane S, Flores C, Zhang Y, Arias AA, Bailey R, Schlüter A, Milisavljevic B, Bigio B, Le Voyer T, Materna M, Gervais A, Moncada-Velez M, Pala F, Lazarov T, Levy R, Neehus AL, Rosain J, Peel J, Chan YH, Morin MP, Pino-Ramirez RM, Belkaya S, Lorenzo L, Anton J, Delafontaine S, Toubiana J, Bajolle F, Fumadó V, DeDiego ML, Fidouh N, Rozenberg F, Pérez-Tur J, Chen S, Evans T, Geissmann F, Lebon P, Weiss SR, Bonnet D, Duval X, Pan-Hammarström Q, Planas AM, Meyts I, Haerynck F, Pujol A, Sancho-Shimizu V, Dalgard CL, Bustamante J, Puel A, Boisson-Dupuis S, Boisson B, Maniatis T, Zhang Q, Bastard P, Notarangelo L, Béziat V, Perez de Diego R, Rodriguez-Gallego C, Su HC, Lifton RP, Jouanguy E, Cobat A, Alsina L, Keles S, Haddad E, Abel L, Belot A, Quintana-Murci L, Rice CM, Silverman RH, Zhang SY, and Casanova JL
- Subjects
- Child, Humans, RNA, Double-Stranded, COVID-19 immunology, Cytokines genetics, Cytokines immunology, Endoribonucleases genetics, Endoribonucleases metabolism, SARS-CoV-2 genetics, Systemic Inflammatory Response Syndrome genetics
- Abstract
Multisystem inflammatory syndrome in children (MIS-C) is a rare and severe condition that follows benign COVID-19. We report autosomal recessive deficiencies of OAS1 , OAS2 , or RNASEL in five unrelated children with MIS-C. The cytosolic double-stranded RNA (dsRNA)-sensing OAS1 and OAS2 generate 2'-5'-linked oligoadenylates (2-5A) that activate the single-stranded RNA-degrading ribonuclease L (RNase L). Monocytic cell lines and primary myeloid cells with OAS1, OAS2, or RNase L deficiencies produce excessive amounts of inflammatory cytokines upon dsRNA or severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) stimulation. Exogenous 2-5A suppresses cytokine production in OAS1-deficient but not RNase L-deficient cells. Cytokine production in RNase L-deficient cells is impaired by MDA5 or RIG-I deficiency and abolished by mitochondrial antiviral-signaling protein (MAVS) deficiency. Recessive OAS-RNase L deficiencies in these patients unleash the production of SARS-CoV-2-triggered, MAVS-mediated inflammatory cytokines by mononuclear phagocytes, thereby underlying MIS-C.
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- 2023
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28. ImmunoTyper-SR: A computational approach for genotyping immunoglobulin heavy chain variable genes using short-read data.
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Ford MKB, Hari A, Rodriguez O, Xu J, Lack J, Oguz C, Zhang Y, Weber S, Magliocco M, Barnett J, Xirasagar S, Samuel S, Imberti L, Bonfanti P, Biondi A, Dalgard CL, Chanock S, Rosen L, Holland S, Su H, Notarangelo L, Vishkin U, Watson CT, and Sahinalp SC
- Subjects
- Humans, Genotype, High-Throughput Nucleotide Sequencing, Immunoglobulin Heavy Chains genetics, Autoantibodies genetics, Immunoglobulin Variable Region genetics, COVID-19 genetics
- Abstract
Human immunoglobulin heavy chain (IGH) locus on chromosome 14 includes more than 40 functional copies of the variable gene (IGHV), which are critical for the structure of antibodies that identify and neutralize pathogenic invaders as a part of the adaptive immune system. Because of its highly repetitive sequence composition, the IGH locus has been particularly difficult to assemble or genotype when using standard short-read sequencing technologies. Here, we introduce ImmunoTyper-SR, an algorithmic tool for the genotyping and CNV analysis of the germline IGHV genes on Illumina whole-genome sequencing (WGS) data using a combinatorial optimization formulation that resolves ambiguous read mappings. We have validated ImmunoTyper-SR on 12 individuals, whose IGHV allele composition had been independently validated, as well as concordance between WGS replicates from nine individuals. We then applied ImmunoTyper-SR on 585 COVID patients to investigate the associations between IGHV alleles and anti-type I IFN autoantibodies, which were previously associated with COVID-19 severity., Competing Interests: Declaration of interests The authors declare no competing interests., (Published by Elsevier Inc.)
- Published
- 2022
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29. Successful Pregnancies, Births, and Children Development Following Oocyte Cryostorage in Female Cancer Patients During 25 Years of Fertility Preservation.
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Porcu E, Cipriani L, Dirodi M, De Iaco P, Perrone AM, Zinzani PL, Taffurelli M, Zamagni C, Ciotti PM, Notarangelo L, Calza N, and Damiano G
- Abstract
The preservation of fertility in cancer patients is a crucial aspect of modern reproductive medicine. Amenorrhea and infertility often occur after cancer therapy, worsening the quality of life. Cryopreservation of oocytes in young cancer patients is a therapeutic option for preserving fertility. A prospective study was conducted on 508 cancer patients who underwent oocyte cryopreservation to preserve fertility between 1996 and 2021 including the COVID-19 pandemic period. Patients underwent ovarian stimulation, followed by egg retrieval, and oocytes were cryopreserved by slow freezing or vitrification. Sixty-four thawing/warming cycles were performed. Survival, fertilization, pregnancy, and birth rate over the thawing/warming cycles were obtained. The data were compared with those from a group of 1042 nononcological patients who cryopreserved supernumerary oocytes. An average of 8.8 ± 6.9 oocytes were retrieved per cycle, and 6.1 ± 4.2 oocytes were cryopreserved. With their own stored oocytes, 44 patients returned to attempt pregnancy. From a total of 194 thawed/warmed oocytes, 157 survived (80%). In total, 100 embryos were transferred in 57 transfer/cycles, and 18 pregnancies were achieved. The pregnancy rate per transfer and pregnancy rate per patient were 31% and 41%, respectively. No statistically significant differences were observed between oncological patients and nononcological patients. A total of 15 babies were born from oncological patients. Children born showed normal growth and development. One minor malformation was detected.
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- 2022
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30. ImmunoTyper-SR: A Novel Computational Approach for Genotyping Immunoglobulin Heavy Chain Variable Genes using Short Read Data.
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Ford M, Hari A, Rodriguez O, Xu J, Lack J, Oguz C, Zhang Y, Weber S, Magglioco M, Barnett J, Xirasagar S, Samuel S, Imberti L, Bonfanti P, Biondi A, Dalgard CL, Chanock S, Rosen L, Holland S, Su H, Notarangelo L, Vishkin U, Watson C, and Sahinalp SC
- Abstract
Human immunoglobulin heavy chain (IGH) locus on chromosome 14 includes more than 40 functional copies of the variable gene (IGHV), which, together with the joining genes (IGHJ), diversity genes (IGHD), constant genes (IGHC) and immunoglobulin light chains, code for antibodies that identify and neutralize pathogenic invaders as a part of the adaptive immune system. Because of its highly repetitive sequence composition, the IGH locus has been particularly difficult to assemble or genotype through the use of standard short read sequencing technologies. Here we introduce ImmunoTyper-SR, an algorithmic method for genotype and CNV analysis of the germline IGHV genes using Illumina whole genome sequencing (WGS) data. ImmunoTyper-SR is based on a novel combinatorial optimization formulation that aims to minimize the total edit distance between reads and their assigned IGHV alleles from a given database, with constraints on the number and distribution of reads across each called allele. We have validated ImmunoTyper-SR on 12 individuals with Illumina WGS data from the 1000 Genomes Project, whose IGHV allele composition have been studied extensively through the use of long read and targeted sequencing platforms, as well as nine individuals from the NIAID COVID Consortium who have been subjected to WGS twice. We have then applied ImmunoTyper-SR on 585 samples from the NIAID COVID Consortium to investigate associations between distinct IGHV alleles and anti-type I IFN autoantibodies which have been linked to COVID-19 severity.
- Published
- 2022
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31. The risk of COVID-19 death is much greater and age-dependent with type I IFN autoantibodies.
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Manry J, Bastard P, Gervais A, Le Voyer T, Rosain J, Philippot Q, Michailidis E, Hoffmann HH, Eto S, Garcia-Prat M, Bizien L, Parra-Martínez A, Yang R, Haljasmägi L, Migaud M, Särekannu K, Maslovskaja J, de Prost N, Tandjaoui-Lambiotte Y, Luyt CE, Amador-Borrero B, Gaudet A, Poissy J, Morel P, Richard P, Cognasse F, Troya J, Trouillet-Assant S, Belot A, Saker K, Garçon P, Rivière JG, Lagier JC, Gentile S, Rosen L, Shaw E, Morio T, Tanaka J, Dalmau D, Tharaux PL, Sene D, Stepanian A, Mégarbane B, Triantafyllia V, Fekkar A, Heath J, Franco J, Anaya JM, Solé-Violán J, Imberti L, Biondi A, Bonfanti P, Castagnoli R, Delmonte O, Zhang Y, Snow A, Holland S, Biggs C, Moncada-Vélez M, Arias A, Lorenzo L, Boucherit S, Anglicheau D, Planas A, Haerynck F, Duvlis S, Nussbaum R, Ozcelik T, Keles S, Bousfiha A, El Bakkouri J, Ramirez-Santana C, Paul S, Pan-Hammarstrom Q, Hammarstrom L, Dupont A, Kurolap A, Metz C, Aiuti A, Casari G, Lampasona V, Ciceri F, Barreiros L, Dominguez-Garrido E, Vidigal M, Zatz M, van de Beek D, Sahanic S, Tancevski I, Stepanovskyy Y, Boyarchuk O, Nukui Y, Tsumura M, Vidaur L, Tangye S, Burrel S, Duffy D, Quintana-Murci L, Klocperk A, Kann N, Shcherbina A, Lau YL, Leung D, Coulongeat M, Marlet J, Koning R, Reyes L, Chauvineau-Grenier A, Venet F, Monneret G, Nussenzweig M, Arrestier R, Boudhabhay I, Baris-Feldman H, Hagin D, Wauters J, Meyts I, Dyer A, Kennelly S, Bourke N, Halwani R, Sharif-Askari F, Dorgham K, Sallette J, Mehlal-Sedkaoui S, AlKhater S, Rigo-Bonnin R, Morandeira F, Roussel L, Vinh D, Erikstrup C, Condino-Neto A, Prando C, Bondarenko A, Spaan A, Gilardin L, Fellay J, Lyonnet S, Bilguvar K, Lifton R, Mane S, Anderson M, Boisson B, Béziat V, Zhang SY, Andreakos E, Hermine O, Pujol A, Peterson P, Mogensen TH, Rowen L, Mond J, Debette S, deLamballerie X, Burdet C, Bouadma L, Zins M, Soler-Palacin P, Colobran R, Gorochov G, Solanich X, Susen S, Martinez-Picado J, Raoult D, Vasse M, Gregersen P, Rodríguez-Gallego C, Piemonti L, Notarangelo L, Su H, Kisand K, Okada S, Puel A, Jouanguy E, Rice C, Tiberghien P, Zhang Q, Casanova JL, Abel L, and Cobat A
- Abstract
SARS-CoV-2 infection fatality rate (IFR) doubles with every five years of age from childhood onward. Circulating autoantibodies neutralizing IFN-α, IFN-ω, and/or IFN-β are found in ~20% of deceased patients across age groups. In the general population, they are found in ~1% of individuals aged 20-70 years and in >4% of those >70 years old. With a sample of 1,261 deceased patients and 34,159 uninfected individuals, we estimated both IFR and relative risk of death (RRD) across age groups for individuals carrying autoantibodies neutralizing type I IFNs, relative to non-carriers. For autoantibodies neutralizing IFN-α2 or IFN-ω, the RRD was 17.0[95% CI:11.7-24.7] for individuals under 70 years old and 5.8[4.5-7.4] for individuals aged 70 and over, whereas, for autoantibodies neutralizing both molecules, the RRD was 188.3[44.8-774.4] and 7.2[5.0-10.3], respectively. IFRs increased with age, from 0.17%[0.12-0.31] for individuals <40 years old to 26.7%[20.3-35.2] for those ≥80 years old for autoantibodies neutralizing IFN-α2 or IFN-ω, and from 0.84%[0.31-8.28] to 40.5%[27.82-61.20] for the same two age groups, for autoantibodies neutralizing both molecules. Autoantibodies against type I IFNs increase IFRs, and are associated with high RRDs, particularly those neutralizing both IFN-α2 and -ω. Remarkably, IFR increases with age, whereas RRD decreases with age. Autoimmunity to type I IFNs appears to be second only to age among common predictors of COVID-19 death.
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- 2022
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32. Two subsequent seminal productions: A good strategy to treat very severe oligoasthenoteratozoospermic infertile couples.
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Ciotti PM, Calza N, Zuffa S, Notarangelo L, Nardi E, Damiano G, Cipriani L, and Porcu E
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- Adult, Female, Humans, Male, Pregnancy, Pregnancy Rate, Retrospective Studies, Sperm Injections, Intracytoplasmic, Oligospermia therapy, Semen Analysis methods, Sexual Abstinence, Sperm Count
- Abstract
Background: Sexual abstinence is considered one of the several factors that influence sperm quality. Recent studies show that a shortening of the abstinence period could be beneficial mostly in oligoasthenoteratozoospermic (OAT) patients., Objective: Retrospective study to verify the efficacy of a second semen sample after a short abstinence to treat severe OAT infertile patients., Materials and Methods: 127 couples treated between May 2014 and May 2018 were divided into two groups. Study Group 1 (75 cycles): severe OAT characteristics: count <0.2 × 10
6 /mL no progressive motility; count ≥0.2 × 106 /mL and no total or progressive motility; 0% normal morphology; a second semen sample was requested after abstinence of 2 h. Control Group 0 (52 cycles): normozoospermic or mild OAT; only one sample was requested. Intracytoplasmic sperm injection was utilized in all cases., Results: All semen parameters were significantly different between Group 0 vs both samples of Group 1 (p < 0.001), excluding volume between Group 0 and 1st sample of Group 1 (p = 0.682). The comparison between 1st and 2nd samples from Group 1 showed significant differences in volume, total and progressive motility and morphology (p < 0.001, p < 0.001, p < 0.020) but not in total sperm count (p = 0.970). Fertilization, pregnancy rate/transfer, implantation and miscarriage rates were 85.9% and 61.1% (p < 0.001), 30.6% and 35.8% (p = 0.700), 17.5% and 24.0 (p = 0.292), 20.0% and 25.0% (p = 0.017) in Group 0 and Group 1 respectively., Discussion and Conclusion: The results show that a short abstinence in severe OAT patients allows us to obtain spermatozoa with better motility. The request for a second semen sample in couples with extreme semen parameters is a valid and simple strategy that helps to achieve the same probability of pregnancy compared to a Control Group. Furthermore, it allows us to utilize fresh spermatozoa avoiding the need to resort to cryopreserved reserves or testicular surgery., (© 2021 American Society of Andrology and European Academy of Andrology.)- Published
- 2021
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33. High-security closed devices are efficient and safe to protect human oocytes from potential risk of viral contamination during vitrification and storage especially in the COVID-19 pandemic.
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Porcu E, Tranquillo ML, Notarangelo L, Ciotti PM, Calza N, Zuffa S, Mori L, Nardi E, Dirodi M, Cipriani L, Labriola FS, and Damiano G
- Subjects
- Adult, Cryopreservation methods, Cryopreservation standards, Embryo Implantation physiology, Embryo Transfer methods, Female, Fertilization in Vitro methods, Fertilization in Vitro standards, Humans, Italy, Oocyte Donation methods, Oocyte Donation standards, Pandemics, Pregnancy, Pregnancy Rate, Prospective Studies, SARS-CoV-2 isolation & purification, Sperm Injections, Intracytoplasmic methods, COVID-19 epidemiology, Oocytes physiology, Oocytes virology, Reproductive Techniques, Assisted standards
- Abstract
Purpose: The main purpose and research question of the study are to compare the efficacy of high-security closed versus open devices for human oocytes' vitrification., Methods: A prospective randomized study was conducted. A total of 737 patients attending the Infertility and IVF Unit at S.Orsola University Hospital (Italy) between October 2015 and April 2020 were randomly assigned to two groups. A total of 368 patients were assigned to group 1 (High-Security Vitrification™ - HSV) and 369 to group 2 (Cryotop® open system). Oocyte survival, fertilization, cleavage, pregnancy, implantation, and miscarriage rate were compared between the two groups., Results: No statistically significant differences were observed on survival rate (70.3% vs. 73.3%), fertilization rate (70.8% vs. 74.9%), cleavage rate (90.6% vs. 90.3%), pregnancy/transfer ratio (32.0% vs. 31.8%), implantation rate (19.7% vs. 19.9%), nor miscarriage rates (22.1% vs. 21.5%) between the two groups. Women's mean age in group 1 (36.18 ± 3.92) and group 2 (35.88 ± 3.88) was not significantly different (P = .297). A total of 4029 oocytes were vitrified (1980 and 2049 in groups 1 and 2 respectively). A total of 2564 were warmed (1469 and 1095 in groups 1 and 2 respectively). A total of 1386 morphologically eligible oocytes were inseminated by intracytoplasmic sperm injection (792 and 594 respectively, P = .304)., Conclusions: The present study shows that the replacement of the open vitrification system by a closed one has no impact on in vitro and in vivo survival, development, pregnancy and implantation rate. Furthermore, to ensure safety, especially during the current COVID-19 pandemic, the use of the closed device eliminates the potential samples' contamination during vitrification and storage.
- Published
- 2021
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34. Opinion and Special Articles: Cerebellar Ataxia and Liver Failure Complicating IPEX Syndrome.
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Rim J, Byler M, Soldatos A, Notarangelo L, Leibovitch E, Jacobson S, Gorman M, Lebel RR, and Werner K
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- Adult, Cerebellar Ataxia etiology, Diabetes Mellitus etiology, Diabetes Mellitus, Type 1 complications, Diabetes Mellitus, Type 1 diagnosis, Diabetes Mellitus, Type 1 genetics, Diarrhea complications, Diarrhea genetics, Forkhead Transcription Factors genetics, Genetic Diseases, X-Linked complications, Genetic Diseases, X-Linked genetics, Humans, Immune System Diseases complications, Immune System Diseases diagnosis, Immune System Diseases genetics, Liver Failure etiology, Magnetic Resonance Imaging, Male, Pedigree, Exome Sequencing, Young Adult, Cerebellar Ataxia diagnosis, Diabetes Mellitus, Type 1 congenital, Diarrhea diagnosis, Genetic Diseases, X-Linked diagnosis, Immune System Diseases congenital
- Published
- 2021
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35. TLR3 controls constitutive IFN-β antiviral immunity in human fibroblasts and cortical neurons.
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Gao D, Ciancanelli MJ, Zhang P, Harschnitz O, Bondet V, Hasek M, Chen J, Mu X, Itan Y, Cobat A, Sancho-Shimizu V, Bigio B, Lorenzo L, Ciceri G, McAlpine J, Anguiano E, Jouanguy E, Chaussabel D, Meyts I, Diamond MS, Abel L, Hur S, Smith GA, Notarangelo L, Duffy D, Studer L, Casanova JL, and Zhang SY
- Subjects
- Animals, Cell Line, Cerebral Cortex pathology, Cerebral Cortex virology, Fibroblasts pathology, Fibroblasts virology, Humans, Interferon-beta genetics, Mice, Mice, Knockout, Neurons pathology, Neurons virology, Toll-Like Receptor 3 genetics, Cerebral Cortex immunology, Fibroblasts immunology, Herpesvirus 1, Human immunology, Interferon-beta immunology, Neurons immunology, Toll-Like Receptor 3 immunology, Vesiculovirus immunology
- Abstract
Human herpes simplex virus 1 (HSV-1) encephalitis can be caused by inborn errors of the TLR3 pathway, resulting in impairment of CNS cell-intrinsic antiviral immunity. Deficiencies of the TLR3 pathway impair cell-intrinsic immunity to vesicular stomatitis virus (VSV) and HSV-1 in fibroblasts, and to HSV-1 in cortical but not trigeminal neurons. The underlying molecular mechanism is thought to involve impaired IFN-α/β induction by the TLR3 recognition of dsRNA viral intermediates or by-products. However, we show here that human TLR3 controls constitutive levels of IFNB mRNA and secreted bioactive IFN-β protein, and thereby also controls constitutive mRNA levels for IFN-stimulated genes (ISGs) in fibroblasts. Tlr3-/- mouse embryonic fibroblasts also have lower basal ISG levels. Moreover, human TLR3 controls basal levels of IFN-β secretion and ISG mRNA in induced pluripotent stem cell-derived cortical neurons. Consistently, TLR3-deficient human fibroblasts and cortical neurons are vulnerable not only to both VSV and HSV-1, but also to several other families of viruses. The mechanism by which TLR3 restricts viral growth in human fibroblasts and cortical neurons in vitro and, by inference, by which the human CNS prevents infection by HSV-1 in vivo, is therefore based on the control of early viral infection by basal IFN-β immunity.
- Published
- 2021
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36. Late-onset and long-lasting autoimmune neutropenia: an analysis from the Italian Neutropenia Registry.
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Fioredda F, Rotulo GA, Farruggia P, Dagliano F, Pillon M, Trizzino A, Notarangelo L, Luti L, Lanza T, Terranova P, Lanciotti M, Ceccherini I, Grossi A, Porretti L, Verzegnassi F, Mastrodicasa E, Barone A, Russo G, Bonanomi S, Boscarol G, Finocchi A, Veltroni M, Ramenghi U, Onofrillo D, Martire B, Ghilardi R, Giordano P, Ladogana S, Marra N, Zanardi S, Beier F, Miano M, and Dufour C
- Subjects
- Adult, Child, Child, Preschool, Congenital Bone Marrow Failure Syndromes, Humans, Italy epidemiology, Registries, Autoimmunity, Neutropenia diagnosis, Neutropenia epidemiology
- Abstract
Primary autoimmune neutropenia (pAN) is typified by onset in early infancy and a mild/moderate phenotype that resolves within 3 years of diagnosis. In contrast, secondary AN is classically an adult disease associated with malignancy, autoimmunity, immunodeficiency, viral infection, or drugs. This study describes a cohort of 79 children from the Italian Registry who, although resembling pAN, did not fully match the criteria for pAN because neutropenia either appeared after age 5 years (LO-Np) or lasted longer than 3 years (LL-Np). These 2 categories compared with classical pAN showed a far inferior rate of resolution (P < .001), lower severity of neutropenia (P = .03), leukopenia (P < .001), lymphopenia (P < .001) with low B+ (P = .001), increased need of granulocyte colony-stimulating factor (P = .04), and increased frequency of autoimmunity over the disease course (P < .001). A paired comparison between LO-Np and LL-Np suggested that LO-Np had a lower rate of resolution (P < .001) and lower white blood cell (P < .001) and lymphocyte (P < .001) values, higher occurrence of apthae (P = .008), and a stronger association with autoimmune diseases/markers (P = .001) than LL-Np, thus suggesting a more pronounced autoimmune signature for LO-Np. A next-generation sequencing panel applied in a small subgroup of LO-Np and LL-Np patients identified variants related to immune dysregulations. Overall, these findings indicate that there are important differences among pAN LL-Np and LO-Np. Forms rising after 3 years of age, with low tendency to resolution, require tight monitoring and extensive immune investigations aimed to early identify underlying immunologic disease., (© 2020 by The American Society of Hematology.)
- Published
- 2020
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37. Impact of BRCA1 and BRCA2 mutations on ovarian reserve and fertility preservation outcomes in young women with breast cancer.
- Author
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Porcu E, Cillo GM, Cipriani L, Sacilotto F, Notarangelo L, Damiano G, Dirodi M, and Roncarati I
- Subjects
- Adolescent, Adult, Breast Neoplasms complications, Breast Neoplasms pathology, Female, Fertility Preservation methods, Humans, Mutation genetics, Oocyte Retrieval, Oocytes growth & development, Oocytes pathology, Ovarian Reserve genetics, Ovulation Induction, Primary Ovarian Insufficiency complications, Primary Ovarian Insufficiency pathology, Young Adult, BRCA1 Protein genetics, BRCA2 Protein genetics, Breast Neoplasms genetics, Primary Ovarian Insufficiency genetics
- Abstract
Purpose: To determine the impact of BRCA1 and BRCA2 mutations on ovarian reserve and fertility preservation outcome. The main purpose and research question of the study is to determine the impact of BRCA1 and BRCA2 mutations on ovarian reserve and fertility preservation outcomes., Methods: Prospective study: 67 breast cancer patients between 18 and 40 years old, undergoing a fertility preservation by means of oocyte storage were considered. Inclusions criteria for the study were age between 18 and 40 years old, BMI between 18 and 28, breast cancer neoplasm stage I and II according to American Joint Committee on Cancer classification (2017) and no metastasis., Exclusion Criteria: age over 40 years old, BMI < 18 and > 28, breast cancer neoplasm stage III and IV and do not performed the BRCA test. A total of 21 patients had not performed the test and were excluded. Patients were divided into four groups: Group A was composed by 11 breast cancer patients with BRCA 1 mutations, Group B was composed by 11 breast cancer patients with BRCA 2 mutations, Group C was composed by 24 women with breast cancer without BRCA mutations, and Group D (control) was composed by 181 normal women., Results: Group A showed significant lower AMH levels compared to Group C and D (1.2 ± 1.1 vs 4.5 ± 4.1 p < 0.05 and 1.2 ± 1.1 vs 3.8 ± 2.5 p < 0.05). BRCA1 mutated patients showed a significant lower rate of mature oocytes (MII) compared to Group C (3.1 ± 2.3 vs 7.2 ± 4.4 p < 0,05) and Group D (3.1 ± 2.3 vs 7.3 ± 3.4; p < 0,05). Breast cancer patients needed a higher dose of gonadotropins compared to controls (Group A 2206 ± 1392 Group B2047.5 ± 829.9 Group C 2106 ± 1336 Group D 1597 ± 709 p < 0,05). No significant differences were found among the groups considering basal FSH levels, duration of stimulation, number of developed follicles, and number of total retrieved oocytes. Regarding BRCA2 mutation, no effect on fertility was shown in this study., Conclusions: The study showed that BRCA1 patients had a higher risk of premature ovarian insufficiency (POI) confirmed by a diminished ovarian reserve and a lower number of mature oocytes suitable for cryopreservation.
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- 2020
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38. Autumn Royal and Egnatia Grape Extracts Differently Modulate Cell Proliferation in Human Colorectal Cancer Cells.
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Gigante I, Milella RA, Tutino V, Debiase G, Notarangelo L, Giannandrea MA, De Nunzio V, Orlando A, D'Alessandro R, Caruso MG, and Notarnicola M
- Subjects
- Antineoplastic Agents, Phytogenic isolation & purification, Apoptosis drug effects, Caco-2 Cells, Caspase 3 genetics, Caspase 3 metabolism, Colorectal Neoplasms genetics, Colorectal Neoplasms metabolism, Colorectal Neoplasms pathology, Extracellular Signal-Regulated MAP Kinases metabolism, Gene Expression Regulation, Neoplastic, HT29 Cells, Humans, Phosphorylation, Plant Extracts isolation & purification, S Phase Cell Cycle Checkpoints drug effects, Antineoplastic Agents, Phytogenic pharmacology, Cell Proliferation drug effects, Colorectal Neoplasms drug therapy, Plant Extracts pharmacology, Vitis chemistry
- Abstract
Objective: Polyphenols extracted by table grape have been demonstrated to decrease cell proliferation in vitro and to exert anti-atherosclerotic and antithrombotic activities, regulating cell functions. A grape polyphenolic profile is affected by climate as well as a grape cultivar. This study was aimed to characterize the berry skin polyphenolic composition, antioxidant activity and antiproliferative properties of two black grape cultivars, Autumn Royal and Egnatia., Methods: The phenolic composition of Grape Skin Extracts (GSEs) was determined by HPLC analyses. The antioxidant activity was determined using DPPH, ABTS and ORAC tests. Caco2, HT29 and SW480 human colon cancer cell lines were used to test the effects of GSEs in vitro. Cell proliferation and cell cycle were assessed with the MTT method and a Muse cell analyzer, respectively. qPCR and Western Blotting analysis were used to evaluate gene and protein expression, respectively., Results: The total polyphenolic content and the total antioxidant capacity were significantly higher in Autumn Royal than in Egnatia. However, table grape Egnatia showed greater ability to affect cell proliferation and apoptosis, as well as to exert a growth arrest in the S phase of the cell cycle, particularly in the Caco2 cell line., Conclusion: These data suggest that the new grape variety Egnatia is an interesting source of phenolic compounds that could be of interest in the food and pharmaceutical industries., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)
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- 2020
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39. B cell-intrinsic requirement for STK4 in humoral immunity in mice and human subjects.
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Moran I, Avery DT, Payne K, Lenthall H, Davies EG, Burns S, Ip W, Oleastro MM, Reisli I, Guner S, Keles S, Notarangelo L, Deenick EK, Goodnow CC, Zahra D, Brink R, Wong M, Tangye SG, Ma CS, and Phan TG
- Subjects
- Animals, Humans, Intracellular Signaling Peptides and Proteins, Mice, Knockout, Mutation, Protein Serine-Threonine Kinases deficiency, Protein Serine-Threonine Kinases genetics, B-Lymphocytes immunology, Immunity, Humoral, Protein Serine-Threonine Kinases immunology
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- 2019
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40. Increased proportions of γδ T lymphocytes in atypical SCID associate with disease manifestations.
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Tometten I, Felgentreff K, Hönig M, Hauck F, Albert MH, Niehues T, Perez R, Ghosh S, Picard C, Stary J, Formankova R, Worth A, Soler-Palacín P, García-Prat M, Allende LM, Gonzalez-Granado LI, Stepensky P, Di Cesare S, Scarselli A, Cancrini C, Speckmann C, Gilmour K, Notarangelo L, Ehl S, and Rohr JC
- Subjects
- Humans, Lymphocyte Count, Cytomegalovirus Infections immunology, Hematologic Diseases immunology, Intraepithelial Lymphocytes immunology, Severe Combined Immunodeficiency immunology
- Abstract
Severe combined immunodeficiencies (SCID) comprise a group of genetic diseases characterized by abrogated development of T lymphocytes. In some case reports of atypical SCID patients elevated proportions of γδ T lymphocytes have been reported. However, it is unknown whether these γδ T cells modulate or reflect the patient's clinical phenotype. We investigated the frequency of elevated γδ T cell proportions and associations with clinical disease manifestations in a cohort of 76 atypical SCID patients. Increased proportions of γδ T lymphocytes were present in approximately 60% of these patients. Furthermore, we identified positive correlations between elevated proportions of γδ T cells and the occurrence of CMV infections and autoimmune cytopenias. We discuss that CMV infections might trigger an expansion of γδ T lymphocytes, which could drive the development of autoimmune cytopenias. We advocate that atypical SCID patients should be screened for elevated proportions of γδ T lymphocytes, CMV infection and autoimmune cytopenias., (Crown Copyright © 2019. Published by Elsevier Inc. All rights reserved.)
- Published
- 2019
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41. Expansion of the Human Phenotype Ontology (HPO) knowledge base and resources.
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Köhler S, Carmody L, Vasilevsky N, Jacobsen JOB, Danis D, Gourdine JP, Gargano M, Harris NL, Matentzoglu N, McMurry JA, Osumi-Sutherland D, Cipriani V, Balhoff JP, Conlin T, Blau H, Baynam G, Palmer R, Gratian D, Dawkins H, Segal M, Jansen AC, Muaz A, Chang WH, Bergerson J, Laulederkind SJF, Yüksel Z, Beltran S, Freeman AF, Sergouniotis PI, Durkin D, Storm AL, Hanauer M, Brudno M, Bello SM, Sincan M, Rageth K, Wheeler MT, Oegema R, Lourghi H, Della Rocca MG, Thompson R, Castellanos F, Priest J, Cunningham-Rundles C, Hegde A, Lovering RC, Hajek C, Olry A, Notarangelo L, Similuk M, Zhang XA, Gómez-Andrés D, Lochmüller H, Dollfus H, Rosenzweig S, Marwaha S, Rath A, Sullivan K, Smith C, Milner JD, Leroux D, Boerkoel CF, Klion A, Carter MC, Groza T, Smedley D, Haendel MA, Mungall C, and Robinson PN
- Subjects
- Congenital Abnormalities diagnosis, Databases, Genetic, Genetic Variation, Humans, Internet, Phenotype, Rare Diseases diagnosis, Whole Genome Sequencing methods, Biological Ontologies, Computational Biology methods, Congenital Abnormalities genetics, Genetic Predisposition to Disease genetics, Knowledge Bases, Rare Diseases genetics
- Abstract
The Human Phenotype Ontology (HPO)-a standardized vocabulary of phenotypic abnormalities associated with 7000+ diseases-is used by thousands of researchers, clinicians, informaticians and electronic health record systems around the world. Its detailed descriptions of clinical abnormalities and computable disease definitions have made HPO the de facto standard for deep phenotyping in the field of rare disease. The HPO's interoperability with other ontologies has enabled it to be used to improve diagnostic accuracy by incorporating model organism data. It also plays a key role in the popular Exomiser tool, which identifies potential disease-causing variants from whole-exome or whole-genome sequencing data. Since the HPO was first introduced in 2008, its users have become both more numerous and more diverse. To meet these emerging needs, the project has added new content, language translations, mappings and computational tooling, as well as integrations with external community data. The HPO continues to collaborate with clinical adopters to improve specific areas of the ontology and extend standardized disease descriptions. The newly redesigned HPO website (www.human-phenotype-ontology.org) simplifies browsing terms and exploring clinical features, diseases, and human genes.
- Published
- 2019
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42. The genetic landscape of severe combined immunodeficiency in the United States and Canada in the current era (2010-2018).
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Dvorak CC, Haddad E, Buckley RH, Cowan MJ, Logan B, Griffith LM, Kohn DB, Pai SY, Notarangelo L, Shearer W, Prockop S, Kapoor N, Heimall J, Chaudhury S, Shyr D, Chandra S, Cuvelier G, Moore T, Shenoy S, Goldman F, Smith AR, Sunkersett G, Vander Lugt M, Caywood E, Quigg T, Torgerson T, Chandrakasan S, Craddock J, Dávila Saldaña BJ, Gillio A, Shereck E, Aquino V, DeSantes K, Knutsen A, Thakar M, Yu L, and Puck JM
- Subjects
- Canada epidemiology, Child, Child, Preschool, Female, Humans, Infant, Infant, Newborn, Male, Severe Combined Immunodeficiency epidemiology, United States epidemiology, Severe Combined Immunodeficiency genetics
- Published
- 2019
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43. Transplant for NEMO: this and much, much more.
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Hickstein DD and Notarangelo L
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- Humans, I-kappa B Kinase, NF-kappa B
- Abstract
Competing Interests: Conflict-of-interest disclosure: The authors declare no competing financial interests.
- Published
- 2017
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44. Pillars Article: The X-Linked Lymphoproliferative Disease Gene Product SAP Regulates Signals Induced through the Co-Receptor SLAM. Nature . 1998. 395: 462-469.
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Sayos J, Wu C, Morra M, Wang N, Zhang X, Allen D, van Schaik S, Notarangelo L, Geha R, Roncarolo MG, Oettgen H, De Vries JE, Aversa G, and Terhorst C
- Subjects
- Adult, Animals, Cloning, Molecular, History, 20th Century, Humans, Jurkat Cells, Lymphocyte Activation, Lymphoproliferative Disorders immunology, Male, Mice, Mice, Inbred C57BL, Sequence Alignment, Signaling Lymphocytic Activation Molecule Associated Protein metabolism, Young Adult, Allergy and Immunology history, Lymphoproliferative Disorders genetics, Mutation genetics, Signaling Lymphocytic Activation Molecule Associated Protein genetics, Signaling Lymphocytic Activation Molecule Family Member 1 metabolism, T-Lymphocytes immunology
- Published
- 2017
45. Recommendations for Screening and Management of Late Effects in Patients with Severe Combined Immunodeficiency after Allogenic Hematopoietic Cell Transplantation: A Consensus Statement from the Second Pediatric Blood and Marrow Transplant Consortium International Conference on Late Effects after Pediatric HCT.
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Heimall J, Buckley RH, Puck J, Fleisher TA, Gennery AR, Haddad E, Neven B, Slatter M, Roderick S, Baker KS, Dietz AC, Duncan C, Griffith LM, Notarangelo L, Pulsipher MA, and Cowan MJ
- Subjects
- Adolescent, Adult, Allografts, Child, Child, Preschool, Consensus, Female, Humans, Infant, Infant, Newborn, Male, Practice Guidelines as Topic, Severe Combined Immunodeficiency immunology, Hematopoietic Stem Cell Transplantation, Severe Combined Immunodeficiency therapy
- Abstract
Severe combined immunodeficiency (SCID) is effectively treated with hematopoietic cell transplantation (HCT), with overall survival approaching 90% in contemporary reports. However, survivors are at risk for developing late complications because of the variable durability of high-quality immune function, underlying genotype of SCID, comorbidities due to infections in the pretransplantation and post-transplantation periods, and use of conditioning before transplantation. An international group of transplantation experts was convened in 2016 to review the current knowledge of late effects seen in SCID patients after HCT and to develop recommendations for screening and monitoring for late effects. This report provides recommendations for screening and management of pediatric and adult SCID patients treated with HCT., (Copyright © 2017 The American Society for Blood and Marrow Transplantation. All rights reserved.)
- Published
- 2017
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46. A prospective study on the natural history of patients with profound combined immunodeficiency: An interim analysis.
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Speckmann C, Doerken S, Aiuti A, Albert MH, Al-Herz W, Allende LM, Scarselli A, Avcin T, Perez-Becker R, Cancrini C, Cant A, Di Cesare S, Finocchi A, Fischer A, Gaspar HB, Ghosh S, Gennery A, Gilmour K, González-Granado LI, Martinez-Gallo M, Hambleton S, Hauck F, Hoenig M, Moshous D, Neven B, Niehues T, Notarangelo L, Picard C, Rieber N, Schulz A, Schwarz K, Seidel MG, Soler-Palacin P, Stepensky P, Strahm B, Vraetz T, Warnatz K, Winterhalter C, Worth A, Fuchs S, Uhlmann A, and Ehl S
- Subjects
- Adolescent, Child, Child, Preschool, Female, Humans, Infant, Male, Prospective Studies, Research Design, Hematopoietic Stem Cell Transplantation, Severe Combined Immunodeficiency immunology, Severe Combined Immunodeficiency pathology, Severe Combined Immunodeficiency therapy
- Abstract
Background: Absent T-cell immunity resulting in life-threatening infections provides a clear rationale for hematopoetic stem cell transplantation (HSCT) in patients with severe combined immunodeficiency (SCID). Combined immunodeficiencies (CIDs) and "atypical" SCID show reduced, not absent T-cell immunity. If associated with infections or autoimmunity, they represent profound combined immunodeficiency (P-CID), for which outcome data are insufficient for unambiguous early transplant decisions., Objectives: We sought to compare natural histories of severity-matched patients with/without subsequent transplantation and to determine whether immunologic and/or clinical parameters may be predictive for outcome., Methods: In this prospective and retrospective observational study, we recruited nontransplanted patients with P-CID aged 1 to 16 years to compare natural histories of severity-matched patients with/without subsequent transplantation and to determine whether immunologic and/or clinical parameters may be predictive for outcome., Results: A total of 51 patients were recruited (median age, 9.6 years). Thirteen of 51 had a genetic diagnosis of "atypical" SCID and 14 of 51 of CID. About half of the patients had less than 10% naive T cells, reduced/absent T-cell proliferation, and at least 1 significant clinical event/year, demonstrating their profound immunodeficiency. Nineteen patients (37%) underwent transplantation within 1 year of enrolment, and 5 of 51 patients died. Analysis of the HSCT decisions revealed the anticipated heterogeneity, favoring an ongoing prospective matched-pair analysis of patients with similar disease severity with or without transplantation. Importantly, so far neither the genetic diagnosis nor basic measurements of T-cell immunity were good predictors of disease evolution., Conclusions: The P-CID study for the first time characterizes a group of patients with nontypical SCID T-cell deficiencies from a therapeutic perspective. Because genetic and basic T-cell parameters provide limited guidance, prospective data from this study will be a helpful resource for guiding the difficult HSCT decisions in patients with P-CID., (Copyright © 2016 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.)
- Published
- 2017
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47. Human CD40 ligand deficiency dysregulates the macrophage transcriptome causing functional defects that are improved by exogenous IFN-γ.
- Author
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Cabral-Marques O, Ramos RN, Schimke LF, Khan TA, Amaral EP, Barbosa Bomfim CC, Junior OR, França TT, Arslanian C, Carola Correia Lima JD, Weber CW, Ferreira JF, Tavares FS, Sun J, D'Imperio Lima MR, Seelaender M, Garcia Calich VL, Marzagão Barbuto JA, Costa-Carvalho BT, Riemekasten G, Seminario G, Bezrodnik L, Notarangelo L, Torgerson TR, Ochs HD, and Condino-Neto A
- Subjects
- Adolescent, Adult, Cells, Cultured, Child, Child, Preschool, Humans, Immunologic Deficiency Syndromes genetics, Immunologic Deficiency Syndromes metabolism, Macrophages immunology, Macrophages metabolism, Macrophages physiology, Male, Monocytes cytology, Mycobacterium tuberculosis, Phagocytosis, Transcriptome drug effects, Young Adult, CD40 Ligand deficiency, Immunologic Deficiency Syndromes immunology, Interferon-gamma pharmacology, Macrophages drug effects
- Abstract
Background: CD40 ligand (CD40L) deficiency predisposes to opportunistic infections, including those caused by fungi and intracellular bacteria. Studies of CD40L-deficient patients reveal the critical role of CD40L-CD40 interaction for the function of T, B, and dendritic cells. However, the consequences of CD40L deficiency on macrophage function remain to be investigated., Objectives: We sought to determine the effect of CD40L absence on monocyte-derived macrophage responses., Methods: After observing the improvement of refractory disseminated mycobacterial infection in a CD40L-deficient patient by recombinant human IFN-γ (rhIFN-γ) adjuvant therapy, we investigated macrophage functions from CD40L-deficient patients. We analyzed the killing activity, oxidative burst, cytokine production, and in vitro effects of rhIFN-γ and soluble CD40 ligand (sCD40L) treatment on macrophages. In addition, the effect of CD40L absence on the macrophage transcriptome before and after rhIFN-γ treatment was studied., Results: Macrophages from CD40L-deficient patients exhibited defective fungicidal activity and reduced oxidative burst, both of which improved in the presence of rhIFN-γ but not sCD40L. In contrast, rhIFN-γ and sCD40L ameliorate impaired production of inflammatory cytokines. Furthermore, rhIFN-γ reversed defective control of Mycobacterium tuberculosis proliferation by patients' macrophages. The absence of CD40L dysregulated the macrophage transcriptome, which was improved by rhIFN-γ. Additionally, rhIFN-γ increased expression levels of pattern recognition receptors, such as Toll-like receptors 1 and 2, dectin 1, and dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin in macrophages from both control subjects and patients., Conclusion: Absence of CD40L impairs macrophage development and function. In addition, the improvement of macrophage immune responses by IFN-γ suggests this cytokine as a potential therapeutic option for patients with CD40L deficiency., (Copyright © 2016 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.)
- Published
- 2017
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48. The Second Pediatric Blood and Marrow Transplant Consortium International Consensus Conference on Late Effects after Pediatric Hematopoietic Cell Transplantation: Defining the Unique Late Effects of Children Undergoing Hematopoietic Cell Transplantation for Immune Deficiencies, Inherited Marrow Failure Disorders, and Hemoglobinopathies.
- Author
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Dietz AC, Duncan CN, Alter BP, Bresters D, Cowan MJ, Notarangelo L, Rosenberg PS, Shenoy S, Skinner R, Walters MC, Wagner J, Baker KS, and Pulsipher MA
- Subjects
- Bone Marrow Diseases genetics, Child, Health Planning Guidelines, Humans, Mass Screening methods, Survivors, Bone Marrow Diseases therapy, Hematopoietic Stem Cell Transplantation adverse effects, Hemoglobinopathies therapy, Immunologic Deficiency Syndromes therapy, Long Term Adverse Effects diagnosis, Long Term Adverse Effects etiology
- Abstract
An international consensus conference sponsored by the Pediatric Blood and Marrow Transplant consortium entitled "Late Effects Screening and Recommendations Following Allogeneic Hematopoietic Cell Transplant for Immune Deficiency and Nonmalignant Hematologic Disease" was held in Minneapolis, Minnesota on May 10, 2016 and May 11, 2016. The purpose of the conference was to address the unmet need for greater understanding of and the screening for long-term complications in the growing population of survivors of transplantation for nonmalignant disorders. The conference focused on transplantation for hemoglobinopathy, immune deficiency, and inherited bone marrow syndromes. A multidisciplinary group of experts in the disease areas and transplantation late effects presented the current state of understanding of how the underlying disease, pretransplantation therapies, and transplantation-related factors uniquely interact to influence the development of late toxicities. Recommendations were put forth by the group for the late effects screening of survivors of transplantation for these nonmalignant disorders. The findings and recommendations that came from this conference will be presented in a series of 6 additional manuscripts in the upcoming months. In this manuscript, we explore the need for screening practices specific to the survivors of transplantation for nonmalignant diseases and the methodologic challenges associated with the study of these patients., (Copyright © 2017 The American Society for Blood and Marrow Transplantation. All rights reserved.)
- Published
- 2017
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49. Unrelated Hematopoietic Cell Transplantation in a Patient with Combined Immunodeficiency with Granulomatous Disease and Autoimmunity Secondary to RAG Deficiency.
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John T, Walter JE, Schuetz C, Chen K, Abraham RS, Bonfim C, Boyce TG, Joshi AY, Kang E, Carvalho BT, Mahajerin A, Nugent D, Puthenveetil G, Soni A, Su H, Cowan MJ, Notarangelo L, and Buchbinder D
- Subjects
- Adolescent, Alleles, Autoimmune Diseases diagnosis, Biomarkers, Granulomatous Disease, Chronic diagnosis, Humans, Immunoglobulins, Intravenous therapeutic use, Immunophenotyping, Infections diagnosis, Infections etiology, Infections therapy, Lymphocyte Count, Mutation, Severe Combined Immunodeficiency diagnosis, T-Lymphocytes immunology, T-Lymphocytes metabolism, Transplantation, Homologous, Treatment Outcome, Autoimmune Diseases complications, Autoimmune Diseases genetics, Granulomatous Disease, Chronic complications, Hematopoietic Stem Cell Transplantation adverse effects, Hematopoietic Stem Cell Transplantation methods, Homeodomain Proteins genetics, Severe Combined Immunodeficiency complications, Severe Combined Immunodeficiency therapy
- Abstract
The use of HLA-identical hematopoietic stem cell transplantation (HSCT) demonstrates overall survival rates greater than 75 % for T-B-NK+ severe combined immunodeficiency secondary to pathogenic mutation of recombinase activating genes 1 and 2 (RAG1/2). Limited data exist regarding the use of HSCT in patients with hypomorphic RAG variants marked by greater preservation of RAG activity and associated phenotypes such as granulomatous disease in combination with autoimmunity. We describe a 17-year-old with combined immunodeficiency and immune dysregulation characterized by granulomatous lung disease and autoimmunity secondary to compound heterozygous RAG mutations. A myeloablative reduced toxicity HSCT was completed using an unrelated bone marrow donor. With the increasing cases of immune dysregulation being discovered with hypomorphic RAG variants, the use of HSCT may advance to the forefront of treatment. This case serves to discuss indications of HSCT, approaches to preparative therapy, and the potential complications in this growing cohort of patients with immune dysregulation and RAG deficiency., Competing Interests: The authors declare that they have no competing interests.
- Published
- 2016
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50. Stem cell transplantation for tetratricopeptide repeat domain 7A deficiency: long-term follow-up.
- Author
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Kammermeier J, Lucchini G, Pai SY, Worth A, Rampling D, Amrolia P, Silva J, Chiesa R, Rao K, Noble-Jamieson G, Gasparetto M, Ellershaw D, Uhlig H, Sebire N, Elawad M, Notarangelo L, Shah N, and Veys P
- Subjects
- Follow-Up Studies, Humans, Inflammatory Bowel Diseases genetics, Time Factors, Treatment Outcome, Genetic Predisposition to Disease genetics, Hematopoietic Stem Cell Transplantation methods, Inflammatory Bowel Diseases therapy, Mutation, Proteins genetics
- Published
- 2016
- Full Text
- View/download PDF
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