1. Effect of epigallocatechin-3-gallate on the increase in type II collagen accumulation in cartilage-like MSC sheets.
- Author
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Sato K, Mera H, Wakitani S, and Takagi M
- Subjects
- Aged, Bone Marrow Cells cytology, Bone Marrow Cells drug effects, Bone Marrow Cells metabolism, Cartilage cytology, Cartilage metabolism, Catechin pharmacology, Cell Differentiation drug effects, Chondrocytes cytology, Chondrocytes metabolism, Collagen Type II genetics, Collagen Type II metabolism, Culture Media chemistry, Culture Media pharmacology, Gene Expression Regulation, Humans, Male, Matrix Metalloproteinase 13 genetics, Matrix Metalloproteinase 13 metabolism, Mesenchymal Stem Cells cytology, Mesenchymal Stem Cells metabolism, Primary Cell Culture, Proteolysis drug effects, Reactive Oxygen Species metabolism, SOX9 Transcription Factor genetics, SOX9 Transcription Factor metabolism, Tissue Engineering, Cartilage drug effects, Catechin analogs & derivatives, Chondrocytes drug effects, Chondrogenesis drug effects, Collagen Type II agonists, Mesenchymal Stem Cells drug effects
- Abstract
With the aim to increase type II collagen content in the scaffold-free cartilage-like cell sheet using human bone marrow mesenchymal stem cells, we examined the effect of epigallocatechin-3-gallate (EGCG) addition to the chondrogenic medium for the cell sheet culture. The addition of EGCG (10 μM) increased the content of type II collagen 2-fold, while the addition did not markedly change the expression level of the genes encoding type II collagen and Sox 9. The reactive oxygen species level in the cells in cell sheets was thought to be too low to suppress the accumulation of type II collagen. On the other hand, the addition of EGCG markedly decreased both the matrix metalloproteinase-13 concentration in the supernatant of cell sheet culture and the type II collagen degradation activity in that supernatant. Taken together, EGCG may enhance the accumulation of type II collagen by suppressing type II collagen degradation.
- Published
- 2017
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