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Effect of epigallocatechin-3-gallate on the increase in type II collagen accumulation in cartilage-like MSC sheets.
- Source :
-
Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 2017 Jun; Vol. 81 (6), pp. 1241-1245. Date of Electronic Publication: 2017 Feb 28. - Publication Year :
- 2017
-
Abstract
- With the aim to increase type II collagen content in the scaffold-free cartilage-like cell sheet using human bone marrow mesenchymal stem cells, we examined the effect of epigallocatechin-3-gallate (EGCG) addition to the chondrogenic medium for the cell sheet culture. The addition of EGCG (10 μM) increased the content of type II collagen 2-fold, while the addition did not markedly change the expression level of the genes encoding type II collagen and Sox 9. The reactive oxygen species level in the cells in cell sheets was thought to be too low to suppress the accumulation of type II collagen. On the other hand, the addition of EGCG markedly decreased both the matrix metalloproteinase-13 concentration in the supernatant of cell sheet culture and the type II collagen degradation activity in that supernatant. Taken together, EGCG may enhance the accumulation of type II collagen by suppressing type II collagen degradation.
- Subjects :
- Aged
Bone Marrow Cells cytology
Bone Marrow Cells drug effects
Bone Marrow Cells metabolism
Cartilage cytology
Cartilage metabolism
Catechin pharmacology
Cell Differentiation drug effects
Chondrocytes cytology
Chondrocytes metabolism
Collagen Type II genetics
Collagen Type II metabolism
Culture Media chemistry
Culture Media pharmacology
Gene Expression Regulation
Humans
Male
Matrix Metalloproteinase 13 genetics
Matrix Metalloproteinase 13 metabolism
Mesenchymal Stem Cells cytology
Mesenchymal Stem Cells metabolism
Primary Cell Culture
Proteolysis drug effects
Reactive Oxygen Species metabolism
SOX9 Transcription Factor genetics
SOX9 Transcription Factor metabolism
Tissue Engineering
Cartilage drug effects
Catechin analogs & derivatives
Chondrocytes drug effects
Chondrogenesis drug effects
Collagen Type II agonists
Mesenchymal Stem Cells drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1347-6947
- Volume :
- 81
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Bioscience, biotechnology, and biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 28485205
- Full Text :
- https://doi.org/10.1080/09168451.2017.1282809