Search

Your search keyword '"Emmanuelle Billon"' showing total 16 results
Start Over Author "Emmanuelle Billon" Publication Year Range Last 10 years
16 results on '"Emmanuelle Billon"'

1. Infection with Influenzavirus A in a murine model induces epithelial bronchial lesions and distinct waves of innate immune-cell recruitment

Author

Frédéric Rivière, Julien Burger, François Lefèvre, Annabelle Garnier, Clarisse Vigne, Jean-Nicolas Tournier, and Emmanuelle Billon-Denis

Subjects
two-photon excitation microscopy, Influenza virus, immune response, flow cytometry, cytokines, live lung slice, Immunologic diseases. Allergy, RC581-607
Abstract

IntroductionInflammatory lesions after Influenza A viruses (IAV) are potential therapeutic target for which better understanding of post-infection immune mechanisms is required. Most studies to evaluate innate immune reactions induced by IAV are based on quantitative/functional methods and anatomical exploration is most often non-existent. We aimed to study pulmonary damage and macrophage recruitment using two-photon excitation microscopy (TPEM) after IAV infection.MethodsWe infected C57BL/6 CD11c+YFP mice with A/Puerto Ricco/8/34 H1N1. We performed immune cell analysis, including flow cytometry, cytokine concentration assays, and TPEM observations after staining with anti-F4/80 antibody coupled to BV421. We adapted live lung slice (LLS) method for ex-vivo intravital microscopy to analyze cell motility.ResultsTPEM provided complementary data to flow cytometry and cytokine assays by allowing observation of bronchial epithelium lesions and spreading of local infection. Addition of F4/80-BV421 staining allowed us to precisely determine timing of recruitment and pulmonary migration of macrophages. Ex-vivo LLS preserved cellular viability, allowing us to observe acceleration of macrophage motility.ConclusionAfter IAV infection, we were able to explore structural consequences and successive waves of innate immune cell recruitment. By combining microscopy, flow cytometry and chemokine measurements, we describe novel and precise scenario of innate immune response against IAV.

Published
2023
Full Text
View/download PDF

2. Long-term systemic and mucosal SARS-CoV-2 IgA response and its association with persistent smell and taste disorders

Author

Jessica Denis, Annabelle Garnier, Laurence Cheutin, Audrey Ferrier, Hawa Timera, Fanny Jarjaval, Carine Hejl, Emmanuelle Billon-Denis, Percy ImmunoCovid group, Damien Ricard, Jean-Nicolas Tournier, Aurélie Trignol, and Marie Mura

Subjects
mucosal immunity, SARS-CoV2 vaccine, dysosmia, dysgeusia, IgA, Spike N-terminal domain, Immunologic diseases. Allergy, RC581-607
Abstract

IntroductionCurrent approved COVID-19 vaccines, notably mRNA and adenoviral vectored technologies, still fail to fully protect against infection and transmission of various SARS-CoV-2 variants. The mucosal immunity at the upper respiratory tract represents the first line of defense against respiratory viruses such as SARS-CoV-2 and is thus critical to develop vaccine blocking human-to-human transmission.MethodsWe measured systemic and mucosal Immunoglobulin A (IgA) response in serum and saliva from 133 healthcare workers from Percy teaching military hospital following a mild infection (SARS-CoV-2 Wuhan strain, n=58) or not infected (n=75), and after SARS-CoV-2 vaccination (Vaxzevria®/Astrazeneca and/or Comirnaty®/Pfizer).ResultsWhile serum anti-SARS-CoV-2 Spike IgA response lasted up to 16 months post-infection, IgA response in saliva had mostly fallen to baseline level at 6 months post-infection. Vaccination could reactivate the mucosal response generated by prior infection, but failed to induce a significant mucosal IgA response by itself. Early post-COVID-19 serum anti-Spike-NTD IgA titer correlated with seroneutralization titers. Interestingly, its saliva counterpart positively correlated with persistent smell and taste disorders more than one year after mild COVID-19.DiscussionAs breakthrough infections have been correlated with IgA levels, other vaccine platforms inducing a better mucosal immunity are needed to control COVID-19 infection in the future. Our results encourage further studies to explore the prognosis potential of anti-Spike-NTD IgA in saliva at predicting persistent smell and taste disorders.

Published
2023
Full Text
View/download PDF

3. A live measles-vectored COVID-19 vaccine induces strong immunity and protection from SARS-CoV-2 challenge in mice and hamsters

Author

Phanramphoei N. Frantz, Aleksandr Barinov, Claude Ruffié, Chantal Combredet, Valérie Najburg, Guilherme Dias de Melo, Florence Larrous, Lauriane Kergoat, Samaporn Teeravechyan, Anan Jongkaewwattana, Emmanuelle Billon-Denis, Jean-Nicolas Tournier, Matthieu Prot, Laurine Levillayer, Laurine Conquet, Xavier Montagutelli, Magali Tichit, David Hardy, Priyanka Fernandes, Hélène Strick-Marchand, James Di Santo, Etienne Simon-Lorière, Hervé Bourhy, and Frédéric Tangy

Subjects
Science
Abstract

Here the authors generate a measles virus-based vaccine expressing SARSCoV-2 spike protein and show immunogenicity and protection in mice and hamsters, including neutralization of circulating variants of concerns in vitro.

Published
2021
Full Text
View/download PDF

4. Boosting chloroplast ribosome biogenesis by a plastidial DEAD-box RNA helicase is critical for high light acclimation

Author

El Batoul Djouani-Tahri, Sreedhar Nellaepalli, Pascaline Auroy, Emmanuelle Billon, Adrien Burlacot, Frédéric Chaux-Jukic, Stéphan Cuiné, Virginie Epting, Marie Huleux, Bart Ghysels, Miriam Schulz-Raffelt, Isabelle Te, Sabine Brugière, Yohann Couté, Yuichiro Takahashi, Yonghua Li-Beisson, and Gilles Peltier

Abstract

Photosynthetic organisms have developed sophisticated strategies to fine-tune light energy conversion to meet the metabolic demand, thereby optimizing growth in fluctuating light environments. Although mechanisms such as energy dissipation, photosynthetic control, or the photosystem II (PSII) damage and repair have been widely studied, little is known about the regulation of protein synthesis capacity during light acclimation. By screening a Chlamydomonas reinhardtii insertional mutant library using chlorophyll fluorescence imaging, we isolated a high chlorophyll fluorescence mutant (hf0) defected in a gene encoding a putative plastid targeted DEAD-box RNA helicase called CreRH22. CreRH22 is rapidly induced upon illumination and belongs to the GreenCut, a set of proteins specific to photosynthetic organisms. While photosynthesis is slightly affected in the mutant under low light (LL), exposure to high light (HL) induces a marked decrease in both PSII and PSI, and a strong alteration of the light-induced gene expression pattern. These effects are explained by the inability of hf0 to increase plastid ribosome amounts under HL. We conclude that CreRH22, by promoting ribosomal RNA precursor maturation in a light-dependent manner, enables the assembly of extra-ribosomes required to synthesize photosystem subunits at a higher rate, a critical step in the acclimation of algae to HL.

Published
2022

7. COVID-19 et vaccination : une dérégulation globale

Author

Jean-Nicolas Tournier and Emmanuelle Billon-Denis

Subjects
education.field_of_study, medicine.medical_specialty, business.industry, Public health, Population, Developing country, Outbreak, General Medicine, 030204 cardiovascular system & hematology, medicine.disease, Measles, General Biochemistry, Genetics and Molecular Biology, Poliomyelitis, Vaccination, 03 medical and health sciences, 0302 clinical medicine, Development economics, Pandemic, Medicine, 030212 general & internal medicine, business, education
Abstract

La COVID-19 est une infection pandémique émergente dont l’importante capacité à se propager dans une population dénuée d’immunité n’est plus à prouver. La première réponse des États à la flambée de COVID-19 fut d’imposer un confinement et des mesures barrières, telles que le port du masque et ladistanciation sociale. Une des répercussions de cette pandémie, en matière de santé publique, fut la suspension ou le ralentissement brusque des campagnes de vaccination des nourrissons, un peu partout dans le monde. Un des effets indirects de la COVID-19 est donc le risque de peser sur la mortalité mondiale, principalementviaune recrudescence de la rougeole et de la poliomyélite, principalement dans les pays en voie de développement. Dans ce chaos potentiel, le seul espoir réside dans le développement rapide d’un vaccin efficace contre le SARS-CoV-2 (severe acute respiratory syndrome-coronavirus-2). Cependant, l’acceptation de ce vaccin par la population n’est pas évidente, car outre les nombreuses inconnues qui vont peser inévitablement dans le cas d’un développement très rapide du vaccin, le scepticisme des hésitants vaccinaux va à nouveau se développer.

Published
2020
Full Text
View/download PDF

8. [COVID-19 and vaccination: a global disruption]

Author

Emmanuelle, Billon-Denis and Jean-Nicolas, Tournier

Subjects
Vaccination Coverage, Immunization Programs, SARS-CoV-2, Pneumonia, Viral, Vaccination, COVID-19, Viral Vaccines, Health Services Accessibility, Disease Outbreaks, Betacoronavirus, Humans, Public Health, Patient Participation, Coronavirus Infections, Pandemics, Measles, Poliomyelitis
Abstract

Coronavirus disease (COVID)-19 is an emerging pandemic infection whose significant ability to spread in a naïve population is well established. The first response of states to the COVID-19 outbreak was to impose lock-down and social barrier measures, such as wearing a surgical mask or social distancing. One of the consequences of this pandemic in terms of public health was the suspension or slowdown of infant vaccination campaigns, in almost all countries. The indirect effects of COVID-19 may therefore weigh on mortality from measles and polio in developing countries. In this pandemic chaos, the only hope lies in the rapid development of an effective vaccine against severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2). However, acceptance of this vaccine has not yet been won, as beyond the many unknowns that will inevitably weigh around such rapid development, skepticism among vaccine hesitants is growing.COVID-19 et vaccination : une dérégulation globale.La COVID-19 est une infection pandémique émergente dont l’importante capacité à se propager dans une population dénuée d’immunité n’est plus à prouver. La première réponse des États à la flambée de COVID-19 fut d’imposer un confinement et des mesures barrières, telles que le port du masque et la distanciation sociale. Une des répercussions de cette pandémie, en matière de santé publique, fut la suspension ou le ralentissement brusque des campagnes de vaccination des nourrissons, un peu partout dans le monde. Un des effets indirects de la COVID-19 est donc le risque de peser sur la mortalité mondiale, principalement via une recrudescence de la rougeole et de la poliomyélite, principalement dans les pays en voie de développement. Dans ce chaos potentiel, le seul espoir réside dans le développement rapide d’un vaccin efficace contre le SARS-CoV-2 (severe acute respiratory syndrome-coronavirus-2). Cependant, l’acceptation de ce vaccin par la population n’est pas évidente, car outre les nombreuses inconnues qui vont peser inévitablement dans le cas d’un développement très rapide du vaccin, le scepticisme des hésitants vaccinaux va à nouveau se développer.

Published
2020

9. Differential serological and neutralizing antibody dynamics after an infection by a single SARS-CoV-2 strain

Author

Jessica Denis, Flora Nolent, Emmanuelle Billon-Denis, Annabelle Garnier, Olivier Gorgé, Noémie Verguet, Isabelle Drouet, Emilie Tessier, Audrey Ferrier-Rembert, Olivier Ferraris, Laurence Cheutin, Clarisse Vigne, Cyril Badaut, Hawa Timera, Anne Depeille Wuille, Laet itia Boutin, Clémence Rougeaux, and Jean-Nicolas Tournier

Subjects
0301 basic medicine, Microbiology (medical), Adult, Male, Infectious Disease Transmission, Patient-to-Professional, genetic structures, IgG, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), 030106 microbiology, Enzyme-Linked Immunosorbent Assay, Case Report, Antibodies, Viral, Serology, 03 medical and health sciences, 0302 clinical medicine, Immune system, Nasopharynx, Medicine, Humans, Clinical severity, 030212 general & internal medicine, Neutralizing antibody, Cross Infection, Strain (chemistry), biology, business.industry, SARS-CoV-2, COVID-19, General Medicine, Middle Aged, Antibodies, Neutralizing, Receptor-binding domain, Titer, Infectious Diseases, nervous system, Immunoglobulin G, Immunology, biology.protein, Female, Antibody, business
Abstract

BackgroundWe report here the case of two coworkers infected by the same SARS-CoV-2 strain, presenting two different immunological outcomes. CaseOne patient presented a strong IgG anti-receptor-binding domain immune response correlated with a low and rapidly decreasing titer of neutralizing antibodies. The other patient had similar strong IgG anti-receptor-binding domain immune response but high neutralizing antibody titers. Discussion and ConclusionThus, host individual factors may be the main drivers of the immune response varying with age and clinical severity.

Published
2020

10. Branched-Chain Amino Acid Catabolism Impacts Triacylglycerol Homeostasis in Chlamydomonas reinhardtii

Author

Yariv Brotman, Ismael Torres-Romero, Yonghua Li-Beisson, Stéphan Cuiné, Gilles Peltier, Bertrand Legeret, Emmanuelle Billon, Fantao Kong, Fred Beisson, Adrien Burlacot, Yuanxue Liang, Alisdair R. Fernie, Saleh Alseekh, Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) (BIAM), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Dalian University of Technology, Bioénergie et Microalgues (EBM), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Max Planck Institute of Molecular Plant Physiology (MPI-MP), Max-Planck-Gesellschaft, ANR-11-IDEX-0001,Amidex,INITIATIVE D'EXCELLENCE AIX MARSEILLE UNIVERSITE(2011), European Project: 664621,H2020,H2020-WIDESPREAD-2014-1,PlantaSYST(2015), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), and Environnement, Bioénergie, Microalgues et Plantes (EBMP)

Subjects
0106 biological sciences, 2. Zero hunger, chemistry.chemical_classification, biology, Physiology, Catabolism, Chemistry, Mutant, Chlamydomonas, Branched-chain amino acid, Chlamydomonas reinhardtii, Plant Science, Metabolism, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, biology.organism_classification, 01 natural sciences, Amino acid, chemistry.chemical_compound, Biosynthesis, Biochemistry, Genetics, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS, 010606 plant biology & botany
Abstract

Nitrogen (N) starvation-induced triacylglycerol (TAG) synthesis, and its complex relationship with starch metabolism in algal cells, has been intensively studied; however, few studies have examined the interaction between amino acid metabolism and TAG biosynthesis. Here, via a forward genetic screen for TAG homeostasis, we isolated a Chlamydomonas (Chlamydomonas reinhardtii) mutant (bkdE1α) that is deficient in the E1α subunit of the branched-chain ketoacid dehydrogenase (BCKDH) complex. Metabolomics analysis revealed a defect in the catabolism of branched-chain amino acids in bkdE1α Furthermore, this mutant accumulated 30% less TAG than the parental strain during N starvation and was compromised in TAG remobilization upon N resupply. Intriguingly, the rate of mitochondrial respiration was 20% to 35% lower in bkdE1α compared with the parental strains. Three additional knockout mutants of the other components of the BCKDH complex exhibited phenotypes similar to that of bkdE1α Transcriptional responses of BCKDH to different N status were consistent with its role in TAG homeostasis. Collectively, these results indicate that branched-chain amino acid catabolism contributes to TAG metabolism by providing carbon precursors and ATP, thus highlighting the complex interplay between distinct subcellular metabolisms for oil storage in green microalgae.

Published
2019

11. Small fluorescence-activating and absorption-shifting tag for tunable protein imaging in vivo

Author

Jérôme Quérard, Frederico M. Pimenta, Nelly Morellet, Julien Rossignol, Sophie Vriz, Ewen Lescop, Carole Gauron, Yong Chen, Michel Volovitch, Thomas Le Saux, Sylvie Maurin, Christian G. Specht, Arnaud Gautier, Ludovic Jullien, Marie-Aude Plamont, Antoine Triller, Buyan Pan, Jian Shi, Emmanuelle Billon-Denis, Karine Moncoq, HAL-UPMC, Gestionnaire, Département de Chimie - ENS Paris, École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), Processus d'Activation Sélective par Transfert d'Energie Uni-électronique ou Radiatif (UMR 8640) (PASTEUR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Département de Chimie - ENS Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Centre interdisciplinaire de recherche en biologie (CIRB), Labex MemoLife, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Collège de France (CdF (institution))-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de biologie de l'ENS Paris (IBENS), Département de Biologie - ENS Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie des Substances Naturelles (ICSN), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université Paris Diderot - Paris 7 (UPD7), École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Collège de France (CdF (institution))-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Collège de France (CdF (institution))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de biologie de l'ENS Paris (UMR 8197/1024) (IBENS), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS Paris), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Département de Biologie - ENS Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS Paris), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0301 basic medicine, Embryo, Nonmammalian, Cell Survival, Recombinant Fusion Proteins, Protein tag, Yeast display, Corrections, Fluorescence, Green fluorescent protein, 03 medical and health sciences, Imaging, Three-Dimensional, fluorescence imaging, [CHIM] Chemical Sciences, Organelle, Animals, Humans, [CHIM]Chemical Sciences, directed evolution, Zebrafish, Multidisciplinary, Staining and Labeling, Chemistry, Absorption, Radiation, Cell sorting, Ligand (biochemistry), Directed evolution, fluorogenic ligand, 030104 developmental biology, Biochemistry, Physical Sciences, Biophysics, Genetic Engineering, HeLa Cells, Subcellular Fractions
Abstract

International audience; This paper presents Yellow Fluorescence-Activating and absorption-Shifting Tag (Y-FAST), a small monomeric protein tag, half as large as the green fluorescent protein, enabling fluorescent labeling of proteins in a reversible and specific manner through the reversible binding and activation of a cell-permeant and nontoxic fluorogenic ligand (a so-called fluorogen). A unique fluorogen activation mechanism based on two spectroscopic changes, increase of fluorescence quantum yield and absorption red shift, provides high labeling selectivity. Y-FAST was engineered from the 14-kDa photoactive yellow protein by directed evolution using yeast display and fluorescence-activated cell sorting. Y-FAST is as bright as common fluorescent proteins, exhibits good photostability, and allows the efficient labeling of proteins in various organelles and hosts. Upon fluorogen binding, fluorescence appears instantaneously, allowing monitoring of rapid processes in near real time. Y-FAST distinguishes itself from other tagging systems because the fluorogen binding is highly dynamic and fully reversible, which enables rapid labeling and unlabeling of proteins by addition and withdrawal of the fluorogen, opening new exciting prospects for the development of multiplexing imaging protocols based on sequential labeling.

Published
2015

12. hCD46 receptor is not required for measles vaccine Schwarz strain replication in vivo: Type-I IFN is the species barrier in mice

Author

Marie Mura, Jean-Nicolas Tournier, Chantal Combredet, Frédéric Tangy, Emmanuelle Billon-Denis, and Claude Ruffié

Subjects
0301 basic medicine, viruses, Measles Vaccine, Mice, Transgenic, Biology, Vaccines, Attenuated, Virus Replication, Measles virus, Membrane Cofactor Protein, 03 medical and health sciences, Mice, Immune system, Species Specificity, Interferon, Virology, Chlorocebus aethiops, medicine, Animals, Humans, Vero Cells, Attenuated vaccine, CD46, Immunogenicity, biology.organism_classification, Mice, Inbred C57BL, 030104 developmental biology, Viral replication, Interferon Type I, Measles vaccine, medicine.drug, Measles
Abstract

Measles virus has been successfully attenuated on chicken embryo cells to obtain a highly efficient and safe live attenuated vaccine, administered thus far to billions of children. Measles virus attenuation has long been described to involve a modification of cellular tropism with the use of human CD46 ubiquitous receptor. Nevertheless, the use of this receptor in vivo is not obvious. In this study we use four different mouse models to decipher the respective part of hCD46 receptor and type-I interferon response in measles host restriction. We observed that only type-I interferon restricts viral replication of attenuated MV Schwarz strain in mice, independently of the presence of hCD46 receptor. By comparing measles virus immunogenicity in the different models, we confirmed that there was no impact on the absence of this receptor on the immune response. Therefore, we propose to simplify the mouse model.

Published
2018

13. Light-Activated Proteolysis for the Spatiotemporal Control of Proteins

Author

Chenge Li, Arnaud Gautier, Ludovic Jullien, Isabelle Aujard, Marie-Aude Plamont, Quentin Delacour, Emmanuelle Billon-Denis, and Thomas Le Saux

Subjects
Proteasome Endopeptidase Complex, Light, Proteolysis, Biology, Biochemistry, Auxin, medicine, Animals, Humans, heterocyclic compounds, Inducer, Light activation, Degradation pathway, chemistry.chemical_classification, Protein function, Photolysis, Indoleacetic Acids, medicine.diagnostic_test, Ubiquitin, fungi, Light activated, food and beverages, General Medicine, Cell biology, HEK293 Cells, chemistry, Molecular Medicine, Degradation (geology)
Abstract

The regulation of proteolysis is an efficient way to control protein function in cells. Here, we present a general strategy enabling to increase the spatiotemporal resolution of conditional proteolysis by using light activation as trigger. Our approach relies on the auxin-inducible degradation system obtained by transposing components of the plant auxin-dependent degradation pathway in mammalian cells. We developed a photoactivatable auxin that acts as a photoactivatable inducer of degradation. Upon local and short light illumination, auxin is released in cells and triggers the degradation of a protein of interest with spatiotemporal control.

Published
2015

14. An algal photoenzyme converts fatty acids to hydrocarbons

Author

Bertrand Légeret, Pavel Müller, Yonghua Li-Beisson, Yohann Couté, David Pignol, Fred Beisson, Gilles Peltier, Damien Sorigué, Emmanuelle Billon, Stéphanie Blangy, Didier Nurizzo, Pascal Arnoux, Pierre Richaud, Solène L. Y. Moulin, Klaus Brettel, Stéphan Cuiné, Sabine Brugière, Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) (BIAM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Synthèse et étude de systèmes à intêret biologique (SEESIB), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Centre National de la Recherche Scientifique (CNRS), Troubles cognitifs dégénératifs et vasculaires (DN2M), Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-INSERM, Bioénergie et Microalgues (EBM), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Etude de la dynamique des protéomes (EDyP), Laboratoire de Biologie à Grande Échelle (BGE - UMR S1038), Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes (UGA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes (UGA), European Synchrotron Radiation Facility (ESRF), Institut de Biologie Intégrative de la Cellule (I2BC), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Saclay, Microbiologie Environnementale et Moléculaire (MEM), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Etude de la dynamique des protéomes (EDyP ), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), ANR-13-JSV5-0005,MUsCA,Ingénierie métabolique d'une microalgue verte en vue de la production d'alcanes à chaine moyenne(2013), ANR-11-IDEX-0001,Amidex,INITIATIVE D'EXCELLENCE AIX MARSEILLE UNIVERSITE(2011), ANR-10-INBS-0005,FRISBI,Infrastructure Française pour la Biologie Structurale Intégrée(2010), ANR-10-INBS-0008,ProFI,Infrastructure Française de Protéomique(2010), Biologie végétale et microbiologie environnementale - UMR7265 ( BVME ), Centre National de la Recherche Scientifique ( CNRS ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Aix Marseille Université ( AMU ), Laboratoire de Biologie à Grande Échelle ( BGE - UMR S1038 ), Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Grenoble Alpes [Saint Martin d'Hères]-Institut National de la Santé et de la Recherche Médicale ( INSERM ), European Synchrotron Radiation Facility ( ESRF ), Biologie et Biotechnologie des Cyanobactéries ( B2CYA ), Département Microbiologie ( Dpt Microbio ), Institut de Biologie Intégrative de la Cellule ( I2BC ), Université Paris-Sud - Paris 11 ( UP11 ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris-Saclay-Centre National de la Recherche Scientifique ( CNRS ) -Université Paris-Sud - Paris 11 ( UP11 ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris-Saclay-Centre National de la Recherche Scientifique ( CNRS ) -Institut de Biologie Intégrative de la Cellule ( I2BC ), Université Paris-Sud - Paris 11 ( UP11 ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris-Saclay-Centre National de la Recherche Scientifique ( CNRS ) -Université Paris-Sud - Paris 11 ( UP11 ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris-Saclay-Centre National de la Recherche Scientifique ( CNRS ), Université Paris-Sud - Paris 11 ( UP11 ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris-Saclay-Centre National de la Recherche Scientifique ( CNRS ), and Environnement, Bioénergie, Microalgues et Plantes (EBMP)

Subjects
0301 basic medicine, Photosynthetic reaction centre, Light, Carboxy-Lyases, Decarboxylation, [SDV]Life Sciences [q-bio], Chlorella, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Alkenes, 010402 general chemistry, 01 natural sciences, Cofactor, 03 medical and health sciences, chemistry.chemical_compound, Oxidoreductase, Alkanes, Phylogeny, ComputingMilieux_MISCELLANEOUS, Plant Proteins, chemistry.chemical_classification, Flavin adenine dinucleotide, Multidisciplinary, biology, [ SDV.BC ] Life Sciences [q-bio]/Cellular Biology, Fatty Acids, Fatty acid, Lipid metabolism, Lipid Metabolism, Photochemical Processes, 0104 chemical sciences, 030104 developmental biology, Enzyme, chemistry, Biochemistry, Biocatalysis, Flavin-Adenine Dinucleotide, biology.protein, Oxidoreductases
Abstract

Algal enzyme driven by blue light Microalgae make hydrocarbons. In searching for the enzyme responsible, Sorigué et al. found a glucose-methanolcholine oxidoreductase (see the Perspective by Scrutton). Expression of the enzyme in Escherichia coli showed that hydrocarbon production requires visible light. In fact, the enzyme requires a constant input of blue photons to carry out its catalytic reaction. A long hydrophobic tunnel in the enzyme stabilizes the fatty acid substrates in proximity to the flavin adenine dinucleotide cofactor. Science , this issue p. 903 ; see also p. 872

Published
2017

15. Hyper-accumulation of starch and oil in a Chlamydomonas mutant affected in a plant-specific DYRK kinase

Author

Pascaline Auroy, Vincent Chochois, Emmanuelle Billon, Yonghua Li-Beisson, David Dauvillée, Miriam Schulz-Raffelt, Stéphan Cuiné, Gilles Peltier, Environnement, Bioénergie, Microalgues et Plantes (EBMP), Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) (BIAM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Support was also provided by the HélioBiotec platform (funded by the European Regional Development Fund, the Région Provence Alpes Côte d’Azur, the French Ministry of Research, and the 'Commissariat à l’Energie Atomique et aux Energies Alternatives')., ANR-08-BIOE-0002,ALGOMICS,ETUDES GLOBALES DE LA CONVERSION ET DU STOCKAGE DE L'ENERGIE CHEZ LES MICROALGUES(2008), ANR-10-BIOE-0004,Algo-H2,Optimisations génétiques, métaboliques, et procédé de la photobioproduction d'hydrogène par la microalgue verte Chlamydomonas reinhardtii(2010), ANR-11-IDEX-0001,Amidex,INITIATIVE D'EXCELLENCE AIX MARSEILLE UNIVERSITE(2011), CNRS, Université de Lille, Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) [BIAM], Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF], Bioénergie et Microalgues (EBM), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), ANR: ALGOH2,Optimisations génétiques, métaboliques, et procédé de photobioproduction d’hydrogène par la microalgue verte Chlamydomonas reinhardtii, and ANR-11-IDEX-0001-02/11-IDEX-0001,AMIDEX,AMIDEX(2011)

Subjects
0301 basic medicine, Kinase, Nutrient deprivation, Oil, Starch, Microalgae, Photosynthesis, Chlamydomonas, DYRK, [SDV]Life Sciences [q-bio], Mutant, Chlamydomonas reinhardtii, Management, Monitoring, Policy and Law, 7. Clean energy, Applied Microbiology and Biotechnology, 03 medical and health sciences, chemistry.chemical_compound, Nutrient, Botany, 2. Zero hunger, biology, Renewable Energy, Sustainability and the Environment, Research, biology.organism_classification, Cell biology, 030104 developmental biology, General Energy, chemistry, Biofuel, Biotechnology
Abstract

Background Because of their high biomass productivity and their ability to accumulate high levels of energy-rich reserve compounds such as oils or starch, microalgae represent a promising feedstock for the production of biofuel. Accumulation of reserve compounds takes place when microalgae face adverse situations such as nutrient shortage, conditions which also provoke a stop in cell division, and down-regulation of photosynthesis. Despite growing interest in microalgal biofuels, little is known about molecular mechanisms controlling carbon reserve formation. In order to discover new regulatory mechanisms, and identify genes of interest to boost the potential of microalgae for biofuel production, we developed a forward genetic approach in the model microalga Chlamydomonas reinhardtii. Results By screening an insertional mutant library on the ability of mutants to accumulate and re-mobilize reserve compounds, we isolated a Chlamydomonas mutant (starch degradation 1, std1) deficient for a dual-specificity tyrosine-phosphorylation-regulated kinase (DYRK). The std1 mutant accumulates higher levels of starch and oil than wild-type and maintains a higher photosynthetic activity under nitrogen starvation. Phylogenetic analysis revealed that this kinase (named DYRKP) belongs to a plant-specific subgroup of the evolutionarily conserved DYRK kinase family. Furthermore, hyper-accumulation of storage compounds occurs in std1 mostly under low light in photoautotrophic condition, suggesting that the kinase normally acts under conditions of low energy status to limit reserve accumulation. Conclusions The DYRKP kinase is proposed to act as a negative regulator of the sink capacity of photosynthetic cells that integrates nutrient and energy signals. Inactivation of the kinase strongly boosts accumulation of reserve compounds under photoautotrophic nitrogen deprivation and allows maintaining high photosynthetic activity. The DYRKP kinase therefore represents an attractive target for improving the energy density of microalgae or crop plants. Electronic supplementary material The online version of this article (doi:10.1186/s13068-016-0469-2) contains supplementary material, which is available to authorized users.

Published
2016

16. Whole genome re-sequencing identifies a quantitative trait locus repressing carbon reserve accumulation during optimal growth in $Chlamydomonas\ reinhardtii$

Author

Emmanuelle Billon, Yonghua Li-Beisson, Fantao Kong, Gilles Peltier, Fred Beisson, Bertrand Legeret, Audrey Beyly-Adriano, Hugh D. Goold, Stéphan Cuiné, Hoa Mai Nguyen, Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) (BIAM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Environnement, Bioénergie, Microalgues et Plantes (EBMP), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), The University of Sydney, OSEO : 'Exploitation Industrielle des Micro-Algues', ANR-12-BIME-0001,DIESALG,Production de biodiesel par microalgues(2012), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), and Bioénergie et Microalgues (EBM)

Subjects
0106 biological sciences, 0301 basic medicine, Light, [SDV]Life Sciences [q-bio], Mutant, Quantitative Trait Loci, Chlamydomonas reinhardtii, Quantitative trait locus, 01 natural sciences, 7. Clean energy, Genome, Genetic analysis, Article, 03 medical and health sciences, Gene, ComputingMilieux_MISCELLANEOUS, 2. Zero hunger, Genetics, Multidisciplinary, biology, Starch, Sequence Analysis, DNA, biology.organism_classification, Carbon, Light intensity, 030104 developmental biology, Biochemistry, Chromosomal region, Mutation, Oils, Metabolic Networks and Pathways, 010606 plant biology & botany
Abstract

Microalgae have emerged as a promising source for biofuel production. Massive oil and starch accumulation in microalgae is possible, but occurs mostly when biomass growth is impaired. The molecular networks underlying the negative correlation between growth and reserve formation are not known. Thus isolation of strains capable of accumulating carbon reserves during optimal growth would be highly desirable. To this end, we screened an insertional mutant library of Chlamydomonas reinhardtii for alterations in oil content. A mutant accumulating five times more oil and twice more starch than wild-type during optimal growth was isolated and named constitutive oil accumulator 1 (coa1). Growth in photobioreactors under highly controlled conditions revealed that the increase in oil and starch content in coa1 was dependent on light intensity. Genetic analysis and DNA hybridization pointed to a single insertional event responsible for the phenotype. Whole genome re-sequencing identified in coa1 a >200 kb deletion on chromosome 14 containing 41 genes. This study demonstrates that, 1), the generation of algal strains accumulating higher reserve amount without compromising biomass accumulation is feasible; 2), light is an important parameter in phenotypic analysis; and 3), a chromosomal region (Quantitative Trait Locus) acts as suppressor of carbon reserve accumulation during optimal growth.

Published
2016

Catalog

Books, media, physical & digital resources
See catalog results