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Hyper-accumulation of starch and oil in a Chlamydomonas mutant affected in a plant-specific DYRK kinase
- Source :
- Biotechnology for Biofuels, Biotechnology for Biofuels, In press, 9 (1), ⟨10.1186/s13068-016-0469-2⟩, Biotechnology for Biofuels, BioMed Central, In press, 9 (1), ⟨10.1186/s13068-016-0469-2⟩
- Publication Year :
- 2016
- Publisher :
- HAL CCSD, 2016.
-
Abstract
- Background Because of their high biomass productivity and their ability to accumulate high levels of energy-rich reserve compounds such as oils or starch, microalgae represent a promising feedstock for the production of biofuel. Accumulation of reserve compounds takes place when microalgae face adverse situations such as nutrient shortage, conditions which also provoke a stop in cell division, and down-regulation of photosynthesis. Despite growing interest in microalgal biofuels, little is known about molecular mechanisms controlling carbon reserve formation. In order to discover new regulatory mechanisms, and identify genes of interest to boost the potential of microalgae for biofuel production, we developed a forward genetic approach in the model microalga Chlamydomonas reinhardtii. Results By screening an insertional mutant library on the ability of mutants to accumulate and re-mobilize reserve compounds, we isolated a Chlamydomonas mutant (starch degradation 1, std1) deficient for a dual-specificity tyrosine-phosphorylation-regulated kinase (DYRK). The std1 mutant accumulates higher levels of starch and oil than wild-type and maintains a higher photosynthetic activity under nitrogen starvation. Phylogenetic analysis revealed that this kinase (named DYRKP) belongs to a plant-specific subgroup of the evolutionarily conserved DYRK kinase family. Furthermore, hyper-accumulation of storage compounds occurs in std1 mostly under low light in photoautotrophic condition, suggesting that the kinase normally acts under conditions of low energy status to limit reserve accumulation. Conclusions The DYRKP kinase is proposed to act as a negative regulator of the sink capacity of photosynthetic cells that integrates nutrient and energy signals. Inactivation of the kinase strongly boosts accumulation of reserve compounds under photoautotrophic nitrogen deprivation and allows maintaining high photosynthetic activity. The DYRKP kinase therefore represents an attractive target for improving the energy density of microalgae or crop plants. Electronic supplementary material The online version of this article (doi:10.1186/s13068-016-0469-2) contains supplementary material, which is available to authorized users.
- Subjects :
- 0301 basic medicine
Kinase
Nutrient deprivation
Oil
Starch
Microalgae
Photosynthesis
Chlamydomonas
DYRK
[SDV]Life Sciences [q-bio]
Mutant
Chlamydomonas reinhardtii
Management, Monitoring, Policy and Law
7. Clean energy
Applied Microbiology and Biotechnology
03 medical and health sciences
chemistry.chemical_compound
Nutrient
Botany
2. Zero hunger
biology
Renewable Energy, Sustainability and the Environment
Research
biology.organism_classification
Cell biology
030104 developmental biology
General Energy
chemistry
Biofuel
Biotechnology
Subjects
Details
- Language :
- English
- ISSN :
- 17546834
- Database :
- OpenAIRE
- Journal :
- Biotechnology for Biofuels, Biotechnology for Biofuels, In press, 9 (1), ⟨10.1186/s13068-016-0469-2⟩, Biotechnology for Biofuels, BioMed Central, In press, 9 (1), ⟨10.1186/s13068-016-0469-2⟩
- Accession number :
- edsair.doi.dedup.....848e2fd1b07b9555e7b55e878d24403a