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Whole genome re-sequencing identifies a quantitative trait locus repressing carbon reserve accumulation during optimal growth in $Chlamydomonas\ reinhardtii$
- Source :
- Scientific Reports, Scientific Reports, 2016, 6, pp.25209. ⟨10.1038/srep25209⟩, Scientific Reports, Nature Publishing Group, 2016, 6 (1), ⟨10.1038/srep25209⟩
- Publication Year :
- 2016
- Publisher :
- HAL CCSD, 2016.
-
Abstract
- Microalgae have emerged as a promising source for biofuel production. Massive oil and starch accumulation in microalgae is possible, but occurs mostly when biomass growth is impaired. The molecular networks underlying the negative correlation between growth and reserve formation are not known. Thus isolation of strains capable of accumulating carbon reserves during optimal growth would be highly desirable. To this end, we screened an insertional mutant library of Chlamydomonas reinhardtii for alterations in oil content. A mutant accumulating five times more oil and twice more starch than wild-type during optimal growth was isolated and named constitutive oil accumulator 1 (coa1). Growth in photobioreactors under highly controlled conditions revealed that the increase in oil and starch content in coa1 was dependent on light intensity. Genetic analysis and DNA hybridization pointed to a single insertional event responsible for the phenotype. Whole genome re-sequencing identified in coa1 a >200 kb deletion on chromosome 14 containing 41 genes. This study demonstrates that, 1), the generation of algal strains accumulating higher reserve amount without compromising biomass accumulation is feasible; 2), light is an important parameter in phenotypic analysis; and 3), a chromosomal region (Quantitative Trait Locus) acts as suppressor of carbon reserve accumulation during optimal growth.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Light
[SDV]Life Sciences [q-bio]
Mutant
Quantitative Trait Loci
Chlamydomonas reinhardtii
Quantitative trait locus
01 natural sciences
7. Clean energy
Genome
Genetic analysis
Article
03 medical and health sciences
Gene
ComputingMilieux_MISCELLANEOUS
2. Zero hunger
Genetics
Multidisciplinary
biology
Starch
Sequence Analysis, DNA
biology.organism_classification
Carbon
Light intensity
030104 developmental biology
Biochemistry
Chromosomal region
Mutation
Oils
Metabolic Networks and Pathways
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Database :
- OpenAIRE
- Journal :
- Scientific Reports, Scientific Reports, 2016, 6, pp.25209. ⟨10.1038/srep25209⟩, Scientific Reports, Nature Publishing Group, 2016, 6 (1), ⟨10.1038/srep25209⟩
- Accession number :
- edsair.doi.dedup.....f3a3750ca9b2bf4f0873732426811c8d