Search

•Alternative antimicrobials should be considered to treat multiresistant Gram-negative bacteria (GNB).•Tigecycline, tetracyclines, ceftazidime/avibactam and fosfomycin are options for multiresistant GNB.•Chloramphenicol and quinolones do not perform well in multiresistant GNB.

•The blaVIM-36gene was identified for the first time in Brazil.•Possibility of spread of this resistance gene in Pseudomonas aeruginosain the region.•Virulence gene profile was similar in isolates carrying blaSPMand the isolate carrying blaVIM-36.•Only two isolates harbouring blaSPMshowed virulence in a Galleria mellonellamodel.

Objective. To describe the analysis of geographical and temporal distribution of DNA profiles determined by pulsed-field gel electrophoresis (PFGE) of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from hospitalized patients in a tertiary care university hospital in Brazil. Methods. Ninety-nine samples of MRSA obtained from 89 patients in the period 1999-2004 were studied. MRSA strains were isolated from central venous catheters (33 isolates) and bloodstream infections (66 strains). PFGE with 20 units of SmaI restriction endonuclease was used for genomic typing. Results. Analysis of DNA PFGE of 99 strains of MRSA revealed 26 profiles and their respective related profiles. The mean time interval for detecting MRSA infection was 26 days from hospital admission. Forty-nine patients (57.6%) had a recent hospitalization. The DNA PFGE MRSA profiles were distributed in three clonal groups--I, II, and III--according to the period of time when the MRSA strains were isolated. DNA PFGE MRSA profiles were spread homogeneously through all hospital wards. Conclusions. Changes in the distribution of DNA PFGE MRSA profiles were largely temporal, with clonal groups being replaced over time, without predominance in any hospital ward or any specific area of the hospital. [ABSTRACT FROM AUTHOR]

Synergy between piperacillin-tazobactam and meropenem against KPC-producing Klebsiella pneumoniaewas recently demonstrated. We sought to test the combination against a broader range of serine carbapenemase producers.

Susceptibility of ceftazidime-avibactam and in vitrosynergy with meropenem were investigated using disk approximation and time-kill assays against 11 multiresistant Acinetobacter baumanniiisolates harboring oxacillinases and 5 Serratia marcescensisolates carrying blaKPC-2. Ceftazidime-avibactam was very active and synergistic with meropenem against multiresistant S. marcescensisolates.