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A patent application titled "Methods For The Treatment Of Cancer" was published online by inventors from France. The application focuses on targeting the recruitment of MDM2 to chromatin in cancer cells, particularly liposarcoma, using Interleukin-6 (IL-6) signaling inhibitors to induce cancer cell death. The method involves administering IL-6 inhibitors to subjects with cancer exhibiting MDM2 recruitment to chromatin, potentially leading to more effective cancer treatments. This patent application aims to address the need for new pharmaceutical compositions and methods to treat and prevent cancers with MDM2 recruitment to chromatin, offering innovative approaches to combat these complex types of cancer. [Extracted from the article]

A recent study from the University of Padua highlights the anti-proliferative and anti-migratory effects of licorice extract and glycyrrhetinic acid on papillary thyroid cancer cell cultures. Licorice, containing over 300 active compounds, shows promise in inhibiting cancer growth. The research found that glycyrrhetinic acid increased apoptosis rates in cell lines, while licorice extract induced oxidative stress. This study provides valuable insights into potential treatments for papillary thyroid cancer. [Extracted from the article]

A patent application has been submitted for the development of genetically-encoded volatile synthetic biomarkers for breath-based cancer detection. The application proposes using limonene, a compound found in citrus fruits, as an indicator of cancer. The biomarkers would be administered to patients through a DNA vector, and the presence of limonene in the breath would indicate the presence and extent of cancer. This technology has the potential to be a non-invasive and cost-effective method for early cancer detection. The patent application provides further details and claims related to the composition and method. [Extracted from the article]

A new report discusses research findings on breast cancer from the University Medical Center Hamburg-Eppendorf in Germany. The study focuses on the impact of the hypoxic tumor microenvironment on cellular behavior and intercellular communication, particularly through extracellular vesicles (EVs). The researchers used nano-LC-MS/MS to analyze the N-glycomes and proteomes of breast cancer cells and their secreted EVs under normoxic and hypoxic conditions. The study found that hypoxia alters the N-glycosylation patterns and glycolysis/gluconeogenesis proteins in cells, and that hypoxia-induced EVs exhibit specific N-glycan signatures associated with poor prognoses for breast tumors. These findings provide insights into potential biomarkers for tracking tumor microenvironment dynamics and developing precision medicine approaches in oncology. [Extracted from the article]

Researchers from the School of Basic Medical Sciences in Anhui, China have developed a three-dimensional vascularized micro-tumor model for screening chemotherapeutic drugs. The model was created using a microencapsulated device to co-culture breast cancer cells and endothelial cells. The researchers found that the three-dimensional culture model was slower in cell proliferation compared to the two-dimensional culture model, and the three-dimensional cultured cells were more resistant to anticancer drugs. This model could provide a platform for drug screening and studying tumor development and angiogenesis. [Extracted from the article]

A new report discusses research findings on colon cancer, specifically focusing on the process of epithelial-mesenchymal transition (EMT) and its role in the metastasis of colorectal cancer cells. The study conducted by Nihon University in Kanagawa, Japan, examined the influence of intercellular contact and epidermal growth factor (EGF) signaling on the induction of EMT in human colon carcinoma cells. The researchers found that EGF can initiate partial EMT in densely cultured cells, leading to reduced levels of E-cadherin, a protein associated with cell adhesion. The study suggests that EGF could be a potential therapeutic target for metastasis in colorectal cancer. [Extracted from the article]

A study conducted at the National University Rio Cuarto in Argentina explored the use of Langmuir and Langmuir-Blodgett films as drug delivery systems to investigate the effects of an anthelmintic drug on cancer cells. The researchers focused on albendazole, a benzimidazole compound with potential anticancer properties. By incorporating albendazole into stable Palmitic Acid Langmuir monolayers, the researchers observed significant effects on the proliferation of liver carcinoma cells. This study provides initial findings on the potential of using albendazole as a surface-mediated drug delivery system for cancer therapy. [Extracted from the article]

A patent application titled "Organs-On-Chips As A Platform For Epigenetics Discovery" has been published online. The inventors, Catherine Karalis and Ville Kujala, highlight the challenges of obtaining relevant epigenetic signatures in humans using animal models or clinical samples. They propose the use of microfluidic devices to identify epigenetic signatures in various sample types, such as cells, immune cells, and tissues. The invention involves exposing cells to extracellular vesicles and detecting their uptake to create diagnostic epigenetic signatures. The method has potential applications in predicting clinical symptoms and studying diseases. [Extracted from the article]

A study conducted by researchers from the Oncode Institute in Utrecht, Netherlands, explores the role of metabolic rewiring in cancer cells and its impact on their ability to proliferate. The researchers highlight the limitations of current in vitro models in accurately reflecting the tumor microenvironment and translating metabolic discoveries into effective clinical therapies. They discuss recent advancements in establishing more physiologically relevant in vitro culture conditions, such as organoids, which may bridge the gap between in vitro and in vivo findings and facilitate the development of innovative metabolic therapies for cancer. The study was funded by the European Research Council and Stichting Kinderen Kankervrij. [Extracted from the article]

Two studies conducted by researchers at Tel Aviv University and the European Institute of Oncology have examined the characteristics of cancer cells with abnormal numbers of chromosomes, known as aneuploid cells. The researchers found that aneuploidy increases the sensitivity of cancer cells to certain types of anticancer drugs. They also discovered vulnerabilities in aneuploid cells, such as an increased activity of DNA repair mechanisms and excess production of RNA and proteins, which can be targeted for new cancer treatments. The findings provide valuable insights for predicting cancer patients' responses to specific drugs and treatments. [Extracted from the article]

Researchers from Dalian University of Technology in China have made progress in the field of cancer treatment by developing a method to deliver singlet oxygen to cancer cell mitochondria. This breakthrough could potentially transform photodynamic therapy (PDT) into a widely applicable treatment for cancer. The researchers designed and synthesized compounds that release singlet oxygen and initiate cell death in cancer cell cultures. In a mouse breast tumor model study, the compounds showed promising results with minimal systemic toxicity. This research has been peer-reviewed and offers new possibilities for cancer treatment. [Extracted from the article]

This article discusses the design of an artificial natural killer (NK) cell mimicking system that can target tumor cells. The system consists of a cell membrane from a human NK cell line and gelatin microspheres as a template. The engineered NK cell mimics were found to be non-toxic, non-inflammatory, and capable of evading macrophage detection. In vitro studies showed significant interaction with cancer cells in various models, and the system was also able to successfully deliver a sialyltransferase inhibitor to reduce sialylation in breast cancer cells. The article emphasizes the potential of these engineered NK cell mimics in cancer research and therapeutic applications. [Extracted from the article]

A recent report from the University of New South Wales highlights the importance of 3D cell cultures in ovarian cancer research. These cultures provide a more comprehensive understanding of the disease, including its high recurrence rates and resistance to chemotherapy. The study emphasizes the need to select the most appropriate model for studying different aspects of ovarian cancer biology, taking into account the strengths and limitations of each approach. The researchers also suggest that using multiple models in combination is likely to be the most effective strategy for studying complex behaviors. [Extracted from the article]

Researchers from Yanshan University in Hebei, China have made progress in the early diagnosis of breast cancer. They used a screening technique called Med-SELEX to identify aptamers, which are molecular recognition elements that can specifically recognize target molecules. Through this process, they identified an aptamer called AY20 that showed high specificity for breast cancer cell culture media. This discovery could potentially lead to the development of a rapid and painless test for breast cancer. The research was supported by the Natural Science Foundation of Hebei Province. [Extracted from the article]

Researchers at Shanghai University of Traditional Chinese Medicine have conducted a study on liver cancer and the therapeutic effects of Jiedu Granule formula (JDF). The study found that JDF reduced the risk of liver cancer metastasis after surgery and inhibited the infiltration of M2 macrophages in liver cancer tissue. In cell experiments, JDF suppressed the activity of M2 macrophages and downregulated the expression of CSF-1 and CSF-1R. It also inhibited the activation of the PI3K/AKT pathway, promoting the apoptosis of M2 macrophages. The study suggests that JDF may hold significant implications for mitigating the risk of metastatic spread in liver cancer patients. [Extracted from the article]

A recent study conducted by researchers at the University of North Texas Health Science Center focused on characterizing estrogen receptor mutant breast cancer in 3D cell culture. The study found that estrogen receptor mutants behave more like triple-negative breast cancer, which is more difficult to treat. The researchers used 3D cell culture to mimic actual tumors and found that the mutants formed larger spheroids compared to wild-type cells. The study highlights the importance of characterizing these mutants before drug treatment studies to better understand how compounds affect cancer cells and improve treatment outcomes. [Extracted from the article]

A patent application has been made available online for a microchip platform that enables high-density hanging drop three-dimensional cell and tissue culture. The inventors highlight the limitations of current 3D culture techniques and propose a modular microchip design that addresses these issues. The platform consists of an array of microwells with fluidic channels for fluid exchange, and an immiscible layer to confine the cells and prevent evaporation or leakage. The microwells can be optically transparent and sensors can be embedded to measure cell or tissue parameters. This platform has potential applications in drug screening, biotechnology, and tissue engineering. [Extracted from the article]

A preprint abstract from biorxiv.org discusses the importance of identifying robust reference genes (RGs) for studying changes in gene expression in response to hypoxia in breast cancer cell lines. Hypoxia, or low oxygen levels, is common in breast tumors and is associated with therapy resistance and advanced disease. The study identified RPLP1 and RPL27 as optimal RGs for breast cancer cell lines cultured in both normoxia and hypoxia. These findings provide a valuable resource for accurately evaluating gene expression in hypoxic breast cancer cell lines and understanding the impact of hypoxia on breast cancer progression. [Extracted from the article]

A report from Zhejiang, People's Republic of China discusses research findings on breast cancer and its metastasis to the bone. The study focuses on the microenvironment of the bone matrix and how its roughness affects the behavior of metastatic breast cancer. The researchers created hydroxyapatite films with distinctive roughness to simulate the bone matrix and observed the adhesion, proliferation, and expression of cytokines related to bone metastasis in breast cancer cells. The study contributes to a better understanding of the impact of the bone matrix microenvironment on breast cancer metastasis. [Extracted from the article]

This article discusses the potential of oncolytic virotherapy as a cancer treatment, specifically focusing on the use of unmodified reovirus T3wt and a mutated variant SV5. The researchers found that SV5 was more effective in targeting and spreading within cancer cells compared to T3wt. They hypothesized that there may be an optimal binding affinity for maximum oncolytic activity and used mathematical modeling to understand the relationship between the binding process and virus spread. The study suggests that a combination of efficient binding and large burst sizes could be a promising direction for developing anti-cancer viruses. However, it is important to note that this preprint has not yet undergone peer review. [Extracted from the article]

A patent application has been filed for a method of obtaining extracellular vesicles (EVs) from cancer cells. EVs are membrane-encapsulated particles that play a role in intercellular communication and have potential therapeutic and diagnostic applications. The inventors propose a three-dimensional cell culture system that allows for the production of EVs that display antigens relevant to specific types of cancer. The method involves cross-linking cancer cells with a substrate, culturing them in a hydrogel, and collecting the EVs secreted by the cells. This approach could provide a valuable source of tumor-specific EVs for further study and the development of targeted diagnosis and therapy. [Extracted from the article]

Sechenov University researchers have conducted a study on targeting EGFR-mediated carcinogenesis, which is the process of cancer development. They studied the antitumor activity of a compound called LHT-17-19 in cell cultures of stomach and breast cancer. The results showed that LHT-17-19 induced cytotoxicity in EGFR-expressing gastric cancer cells and inhibited the growth and proliferation of breast cancer cells. This study suggests that LHT-17-19 has the potential to control the oncogenic expression of EGFR in tumor cells. [Extracted from the article]

Researchers at Osaka University have developed a new therapeutic method for treating neuroendocrine tumors, including small cell lung cancer and neuroendocrine prostate cancer. These tumors are aggressive and often resistant to current treatment options. The researchers focused on a process called RNA splicing, which can contribute to disease development when incorrect. They used molecules called splice-switching oligonucleotides (SSOs) to correct the splicing patterns of a protein called REST, which is expressed at high levels in neuroendocrine tumors. The treatment led to reduced tumor size and decreased expression of genes typically repressed by REST, showing promise for treating these types of cancers. [Extracted from the article]

Oregon State University researchers have discovered compounds that can convert the Bcl-2 protein, which protects cancer cells, into a tumor killer. This breakthrough could lead to promising therapies for breast cancer and other malignancies that rely on Bcl-2 to evade treatment and develop drug resistance. The researchers found that changing the shape of the Bcl-2 molecule exposes its "killer conformation," leading to cell death. The compounds have shown success in cell cultures and mouse models of triple-negative breast cancer, one of the most aggressive forms of the disease. Further research and testing are needed before these compounds can be used in human trials. [Extracted from the article]

A study conducted at Montana State University aimed to develop a three-dimensional bioprinted platform for testing pancreatic cancer drugs. The researchers tested different natural polymer hydrogel formulations and found that a cellulose nanocrystal and alginate hydrogel provided the best printability and cell viability. However, when a spinning bioreactor was used to simulate vasculature, it led to a decrease in cell viability compared to stagnant cultures. Overall, the platform showed promise for future use in pancreatic cancer drug testing. The research has been peer-reviewed and published in Materials Today Communications. [Extracted from the article]

A recent study conducted by researchers at the University of Texas Austin explored the impact of extracellular matrix (ECM) fiber orientation on breast cancer cellular metabolism. The study found that aligned cellular microenvironments play a significant role in promoting metastatic phenotypes of breast cancer, as evidenced by a more glycolytic metabolic signature on nanofiber scaffolds of aligned orientation compared to scaffolds of random orientation. This finding is particularly relevant for subsets of breast cancer marked by high levels of collagen remodeling, such as pregnancy-associated breast cancer, and may serve as a platform for predicting clinical outcomes within these subsets. The study utilized non-invasive imaging techniques to track changes in cellular metabolism and expression levels of metastatic markers. [Extracted from the article]

A preprint abstract from biorxiv.org discusses the influence of cell-cell contacts on extracellular signal-regulated kinase (ERK) signaling in cancer cells. The study used a method called contactBlot, which combines high-content, whole-cell imaging with western blot analysis to measure the phosphorylation level of ERK in individual HeLa cancer cells. The researchers found that ERK signaling was attenuated in HeLa cells that were in contact with other cells compared to isolated cells, and this attenuation was sustained even under hyperosmotic stress. This study provides insights into the impact of cell-cell contacts on ERK activation and introduces a multi omics tool for studying cell-cell interactions. [Extracted from the article]

Researchers from Xi'an Jiaotong-Liverpool University and other institutes in China have developed a molecule called dp53m, which targets a specific mutant version of the tumour protein P53 (TP53) called p53-R175H. This mutation is associated with lung, colorectal, and breast cancers, and can make tumours resistant to chemotherapy. The molecule, a proteolysis-targeting chimera (PROTAC), binds to the mutant protein and recruits an enzyme to break it down. Laboratory tests showed that dp53m inhibited the growth of cancer cells with the p53-R175H mutation, increased their sensitivity to chemotherapy, and did not harm healthy cells. This research suggests a potential therapeutic strategy for diseases associated with the p53-R175H mutation. [Extracted from the article]

Researchers at the National Institute of Technology in Aichi, Japan have developed a cancer cell aggregate culture device that allows for the observation of nutrient and oxygen gradients within three-dimensional cell culture spheroids. The device uses an alginate gel fiber to create a flow path for supplying the culture medium, generating a gradient of nutrients and oxygen. The researchers successfully observed the formation of a hypoxic and hyponutrient region within the spheroid, demonstrating the potential of the device for studying different nutrition and oxygen states in cancer cells. The study was published in the journal Micromachines. [Extracted from the article]

A recent study conducted at Oklahoma State University has found that ginger root and its bioactive compound, 6-gingerol, have the potential to inhibit the growth of colon cancer cells. The researchers used ginger juice and 6-gingerol to treat colon cancer cells in culture and found that both substances were effective in inhibiting the growth of these cells. Importantly, the ginger juice and 6-gingerol did not have the same inhibitory effect on normal colon epithelial cells. These findings suggest that ginger may have potential as a preventive measure against colon cancer. [Extracted from the article]

A recent study conducted at Indiana University-Purdue University has explored the mechanics of the tumor microenvironment (TME) and its impact on cancer progression and the effectiveness of anti-cancer treatments. The researchers developed synthetic polymer hydrogels that mimic the dynamic properties of the TME, allowing for the culture of patient-derived pancreatic cancer cells. The hydrogels were found to induce a mesenchymal phenotype in cancer cells and restrict tumor spheroid growth. This research provides valuable insights into the development of advanced in vitro cancer models for improved cancer research. [Extracted from the article]

Researchers at McGill University Health Centre Research Institute have developed a nanoparticle-functionalized acrylic bone cement for the local delivery of the chemotherapeutic drug doxorubicin (DOX) to spine metastases. The cement was tested for its ability to suppress cancer cells and inhibit their migration. The study found that the addition of nanoparticles to the bone cement achieved sustained release of DOX and inhibited cancer cells in a dose- and time-dependent manner, with minimal toxicity against healthy cells. The researchers concluded that the nanoparticle-functionalized cement has strong potential for further in vivo translation studies. [Extracted from the article]

A recent report discusses research conducted at the University of Texas MD Anderson Cancer Center on the role of the translation elongation factor eEF1A in breast cancer. The study found that eEF1A2 can have either oncogenic or tumor-suppressor functions depending on its interaction with other proteins. Specifically, eEF1A2 was found to promote the degradation of the mitosis-promoting Aurora kinase A, which is associated with increased cell cycling in breast cancer. The researchers suggest that targeting this pathway could be a potential therapeutic strategy for breast cancer treatment. [Extracted from the article]

A study conducted at Sivas Cumhuriyet University examined the effects of Mebendazole, a drug used to treat parasitic infections, on apoptosis (cell death) in cancer cells. The researchers compared the effects of Mebendazole alone and in combination with vincristine and paclitaxel, two other drugs used in cancer treatment. The study found that Mebendazole suppressed cell proliferation, increased levels of caspase enzymes involved in apoptosis, and induced apoptotic DNA formation in lung cancer cells. The researchers concluded that Mebendazole may have therapeutic potential against cancer cells due to its apoptosis-inducing and cell proliferation-suppressive effects. [Extracted from the article]

A recent study conducted by researchers at the Medical University of Silesia in Katowice, Poland, explored the influence of genistein on free radicals in breast cancer cells. The study used electron paramagnetic resonance (EPR) spectroscopy to analyze the levels of free radicals in non-irradiated and UV-irradiated breast cancer cells. The results showed that genistein had varying effects on different types of breast cancer cells, with some concentrations of genistein decreasing the amount of free radicals. The study suggests that the antioxidant efficacy of genistein may be influenced by the subtype of breast cancer cells and the activation of oxidative stress response pathways. [Extracted from the article]

A recent report from California State University discusses the role of Palladin isoform 3 (iso3) in pancreatic cancer cell cultures. The researchers aimed to understand the relationship between iso3 and pancreatic cancer by studying cell models grown in different culture environments. They found that iso3 was overexpressed in 3D cultures of invasive pancreatic cancer cells, suggesting it may be a valuable biomarker for aggressive forms of the disease. The study also highlighted the importance of extracellular viscosity in generating reproducible 3D spheroids. Further research is needed to explore the potential of iso3 as a target for pancreatic cancer treatment. [Extracted from the article]

A recent report from the National Institute for Materials Science in Tsukuba, Japan, explores the impact of hydrogel stiffness on the chemoresistance of breast cancer cells. The study found that the viability of breast cancer cells in hydrogels was dependent on both the concentration of the chemotherapy drug doxorubicin (DOX) and the stiffness of the hydrogel. When the DOX concentration was the same, breast cancer cells showed higher viability in high-stiffness hydrogels compared to low-stiffness hydrogels. The research suggests that hydrogel stiffness can affect the resistance of breast cancer cells to DOX by regulating the expression of chemoresistance-related genes. [Extracted from the article]

A recent preprint abstract discusses the challenges in treating aggressive cancers, particularly pancreatic cancer, and the limited options for therapeutic strategies. The study highlights the importance of 3D cultures in vitro, which can better mimic the mitochondrial oxidative phosphorylation pathways observed in vivo. By reconstructing the genotype of these pathways in 3D cultures, researchers can more accurately analyze and target disease-relevant pathways in pancreatic and other tumors. However, it is important to note that this preprint has not yet undergone peer review. [Extracted from the article]

A study conducted at Taras Shevchenko National University of Kyiv in Ukraine has explored the potential of photocontrolled, biologically active compounds as drug candidates for cancer treatment. These compounds can be activated by non-ionizable light, providing a safer alternative to systemic chemotherapy. The study evaluated the potency and efficiency of these compounds in vitro and ex vivo, as well as their efficacy in vivo during early stages of drug development. The research utilized various cell cultures, tissue surrogates, and animal models to determine the most effective compounds and optimal light dosage. This study offers promising insights into the development of photopharmacology for cancer treatment. [Extracted from the article]

A preprint abstract from biorxiv.org discusses the potential of stimuli-responsive protein vesicles for delivering therapeutic biomacromolecules into cells. These vesicles, made from elastin-like polypeptide (ELP) and modified with histidine and tyrosine residues, were able to deliver cytochrome c and siRNA cargos to cancer cells, resulting in reduced viability. The study also examined the uptake of fluorescent protein cargos and found that hydrophobic ion pairing (HIP) was necessary for cargo release and influenced vesicle self-assembly. This research has the potential to expand the use of intracellular proteins for various diseases and broaden the application of protein vesicles for delivering biomacromolecules. However, it is important to note that this preprint has not yet undergone peer review. [Extracted from the article]

A recent study conducted at the MetroHealth System in Cleveland, Ohio, has explored the use of advanced 3D culture models to study exosomes in triple-negative breast cancer (TNBC). TNBC is a highly aggressive subtype of breast cancer that lacks targeted therapeutics, making it challenging to treat. The researchers found that 3D culture systems, such as organoids and spheroids, provide more accurate representations of in vivo tumors compared to traditional 2D cultures. Exosomes, small molecules secreted by breast cancer cells, play a crucial role in cell-to-cell communication and tumor progression. The study suggests that 3D exosome models could help address drug resistance and improve therapeutic outcomes in breast cancer treatment. [Extracted from the article]

A study conducted at Uijeongbu Eulji Medical Center in South Korea explored the impact of silencing three specific genes (HSP27, cFLIP, and CLU) on the progression of prostate cancer cells and their response to doxazosin-induced apoptosis. The researchers found that silencing the CLU gene significantly reduced cell viability after treatment with doxazosin, and dual silencing of cFLIP and CLU also decreased cell viability. However, the most effective approach was silencing all three genes, which reduced cell viability even at a lower concentration of doxazosin. These findings suggest that simultaneous silencing of these genes could be a potential strategy for promoting apoptosis in prostate cancer cells. [Extracted from the article]

A preprint abstract from biorxiv.org discusses the potential anti-tumor effects of Jianpi Jiedu Recipe (JPJDR) on colon cancer. JPJDR is a traditional Chinese medicine that has been used to treat digestive tract tumors for centuries. The study found that JPJDR inhibited the proliferation of colorectal cancer cells and reduced the expression of PD-L1, a protein associated with cancer growth. It was also shown to induce mitochondrial dysfunction and incomplete autophagy, a process that helps regulate cell growth. The research suggests that JPJDR could be a low-toxic and effective treatment for colon cancer, both in vitro and in vivo. However, it's important to note that this preprint has not been peer-reviewed. [Extracted from the article]

The article focuses on the integration of microbiology and technology in enhancing cancer diagnosis and treatment. Topics include the role of microbes in producing biomarkers and influencing immune responses, advancements in technologies like next-generation sequencing and immunotherapy, and the development of personalized treatments through the study of microbiomes and tumor interactions.

Human mesenchymal stem cells, such as human adipose-derived stem cells (hASCs), are typically cultured on a two-dimensional (2D) monolayer material surface, on which 2D culturing methods are easily performed and time-saving. However, hASCs usually suffer from decreased pluripotency and differentiation ability when cultured with a 2D monolayer culturing method compared to hASCs cultured with a three-dimensional (3D) culturing method, such as suspension culture. In this study, we evaluated whether the pluripotency and differentiation ability of hASCs can be reversibly changed during sequential cultivation with 2D and 3D culturing processes. The hASCs cultivated with a 3D culturing process after 2D culture showed at least 2-fold enhanced pluripotency (Sox2 , Nanog , and OCT4) compared with that of hASCs cultured with the 2D culture process alone. Furthermore, hASCs obtained from the 3D culture process expressed increased levels of differentiation markers of chondrocytes and osteoblasts compared with hASCs obtained from the 2D culture process when hASCs were induced to differentiate. However, their pluripotency and differentiation ability were extensively reduced when hASCs were shifted from 3D culture to 2D culture and vice versa, which indicates that hASCs show reversibility in terms of their pluripotency and differentiation ability depending on their environment in 2D and 3D culture. The reversibility of pluripotency and differentiation ability were found to last for at least 5 passages in culture during the alternative and sequential culture of cells with 2D and 3D culturing processes. Our study revealed the importance of the culture microenvironment in maintaining the pluripotency and differentiation ability of hASCs, which may reduce the effects of the aging process in hASCs. We discuss whether the environment of stem cell culture (i.e., 2D or 3D cultivation) can affect stem cell fate in terms of pluripotency and differentiation reversibility. [ABSTRACT FROM AUTHOR]

When monkey kidney cells are used, the exchange of genetic material that takes place forces the human virus to become a little bit "monkey-like", supposedly so that it cannot initiate full-blown disease - which doesn't always work. For example, a monkey virus called SV40 (simian virus 40, after becoming the 40th virus found in monkey tissues) was found to have unique properties. This "naked virus" can penetrate any kind of cell without the problem of a "species barrier", and it allows unlike viruses to attach to it and ride piggyback into the genetic material of a cell where they can take over the "machinery" and replicate new recombined viruses on their own. These same monkey cell cultures, used to make vaccines, contained other viruses such as SIV (simian immuno-deficiency virus), which figures prominently in the make-up of another recombinant virus that we know as HIV. [Extracted from the article]

A recent study conducted by the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures in Braunschweig, Germany, has provided new insights into breast cancer. The researchers analyzed a panel of 29 breast cancer cell lines and found that they could be categorized into two major clusters representing basal-like and luminal subtypes. The study also identified frequent mutations in TP53 and BRCA2 in these cell lines, as well as potential oncogenic fusion transcripts and candidate tumor suppressor genes. The findings of this study may help improve the selection of appropriate models for breast cancer research. [Extracted from the article]

A report from Sharjah, United Arab Emirates discusses the use of scaffold-based 3D cell culture models in cancer research. These models provide a more accurate representation of the tumor microenvironment and allow for the study of tumor behavior and response to therapies in a physiologically relevant context. The 3D cell cultures bridge the gap between traditional 2D cell cultures and animal models, offering a cost-effective and ethical alternative for preclinical research. The article highlights the advantages of 3D cell cultures, progress in scaffold-based culturing techniques, and ongoing challenges in the field. [Extracted from the article]