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This study aimed to determine the antimicrobial activity of culture fluid and mycelial mass of selected Hericium strains from the IBK Mushroom Culture Collection (Kyiv, Ukraine) against bacteria and fungi, as well as to evaluate the potential of these mushrooms for biomass and extracellular polysaccharides production.

The majority of research programs had been focused on extract from the fruiting body. However, fungal mycelium obtained by submerged cultivation also has higher pharmacological potential. The aim of this study was investigation of the antioxidant and antimicrobial potential of Ganoderma lucidum and Trametes versicolor fungal mycelium biomass obtained by submerged cultivation and liquid fluid.

The effect of different submerged culture conditions (medium composition, pH and temperature) on mycelium biomass and production of intracellular polysaccharides (IPS) by the valuable edible and medicinal mushroom Lentinula edodes was investigated. In total, 12 carbon and 7 nitrogen sources in the nutrient medium were tested. Glucose and peptone were determined to be the most suitable carbon and nitrogen sources, respectively, both for IPS and biomass production. Generally, modified glucose-peptone medium, C/N ratio of 18, pH adjusted to 6.0 and cultivation temperature of 25 °C were found to enhance the yield of biomass and IPS by mycelium of L. edodes IBK 2541 in submerged culture. Selection of medium composition and physical cultivation parameters resulted in an IPS production of 940 mg/l on the modified glucose-peptone medium, which is three-fold higher than that from the basalmedium (306mg/l). [ABSTRACT FROM AUTHOR]

Cells catalysis is efficient methodology that has been extensively applied in various biological processes. However, industrial strains are vulnerable to environmental change, leading to poor stability and productivity. In this regards, large potentialities are embedded in immobilized cells. In particular, the immobilization techniques are of great significance in improving the catalytic performance of natural biocatalysts. Effective method of enzyme production by immobilization of microbial cells on solid career in submerged conditions has been developed. It was determined that design of proposed equipment gives the opportunity to increase enzymatic activity of immobilized cells compared to free cells by several times. A cultivation of Aspergillus oryzae M has been carried out for 49 days by immobilization of fungal cells in submerged conditions of growth. Enzymatic activity was enhanced significantly after 6 days of cultivation of immobilized cells and keeps the same value for 49 days of fungal cultivation. The alpha-amylase activity has been increased to 696 U/ml. [ABSTRACT FROM AUTHOR]