Effects of cultivation parameters on intracellular polysaccharide production in submerged culture of the edible medicinal mushroom Lentinula edodes.

The effect of different submerged culture conditions (medium composition, pH and temperature) on mycelium biomass and production of intracellular polysaccharides (IPS) by the valuable edible and medicinal mushroom Lentinula edodes was investigated. In total, 12 carbon and 7 nitrogen sources in the nutrient medium were tested. Glucose and peptone were determined to be the most suitable carbon and nitrogen sources, respectively, both for IPS and biomass production. Generally, modified glucose-peptone medium, C/N ratio of 18, pH adjusted to 6.0 and cultivation temperature of 25 °C were found to enhance the yield of biomass and IPS by mycelium of L. edodes IBK 2541 in submerged culture. Selection of medium composition and physical cultivation parameters resulted in an IPS production of 940 mg/l on the modified glucose-peptone medium, which is three-fold higher than that from the basalmedium (306mg/l). [ABSTRACT FROM AUTHOR]