Cell immobilization for efficient enzymes production.

Cells catalysis is efficient methodology that has been extensively applied in various biological processes. However, industrial strains are vulnerable to environmental change, leading to poor stability and productivity. In this regards, large potentialities are embedded in immobilized cells. In particular, the immobilization techniques are of great significance in improving the catalytic performance of natural biocatalysts. Effective method of enzyme production by immobilization of microbial cells on solid career in submerged conditions has been developed. It was determined that design of proposed equipment gives the opportunity to increase enzymatic activity of immobilized cells compared to free cells by several times. A cultivation of Aspergillus oryzae M has been carried out for 49 days by immobilization of fungal cells in submerged conditions of growth. Enzymatic activity was enhanced significantly after 6 days of cultivation of immobilized cells and keeps the same value for 49 days of fungal cultivation. The alpha-amylase activity has been increased to 696 U/ml. [ABSTRACT FROM AUTHOR]