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The growth of functional components containing agricultural foods is enhancing because these components aid the human body against different chronic diseases. Currently, chia seeds basically belong to the mint family and are edible seeds of Salvia hispanica. These seeds are composed of different functional components including fiber, polyphenols, antioxidants, omega‐3 fatty acid vitamins, minerals, and peptides. Besides, these seeds are also a good source of vegetable protein, unsaturated fat, carbohydrates, and ash. Chia seed components are helpful in cardiovascular disease (CVD) by reducing blood pressure, platelet aggregation, cholesterol, and oxidation. In GI‐tract‐related diseases like diabetes and constipation, chia fiber reduces the blood glucose level and provides bulk to stool. However, antioxidants and polyphenols are protected beta cells of the pancreas from inflammation. These components are protected from the cell damage of the different body parts, which can provide help in different types of cancer including breast, colorectal, liver, and pancreatic. Conclusively, some pervious studies approved that chia seed components are played important role in chronic diseases. [ABSTRACT FROM AUTHOR]

The viscoelastic properties of dough prepared from the wheat flour are due to the presence of high molecular weight subunits of the protein glutenin (HMW-GS) present in the wheat endosperm. The large numbers of hydrogen bonds present centrally in HMW subunits are responsible for the elastic properties.

The objective of the study was to assess the effect of exogenous administration of oxytocin on gross composition and mineral contents in milk of Sahiwal cow. At three lactation stages, milk samples were collected from two groups of sixteen animals each under controlled atmosphere and feeding conditions. One group was subjected to intramuscular injection of oxytocin (20 IU) and other was kept as control. Significant variations were obtained in milk composition along with lactations stages. Decrease in fat, protein, lactose, solids not fat and total solids contents and increase in ash contents were noted in oxytocin administreted milk. Minerals’ analysis of the milk samples were conducted and it was found that lactation stages have significant effect on minerals composition i.e. macro minerals (Na, Cl, K, Ca, Mg and P) and micro minerals (Zn and Cu) in milk. Oxytocin administration showed significant effect on milk minerals during various lactation stages as sodium, chloride and copper contents increased while potassium decreased. It was concluded that indiscriminate use of oxytocin for milk let down considerably influences minerals profile and results in detrimental variations of gross composition of milk. [ABSTRACT FROM AUTHOR]

The cell wall is a non-starch polysaccharide and consists of various bioactive moieties. In addition, it has many functional and nutraceutical properties. The main purpose of this present research was to extract the cell wall from different cereal bran and to characterize it for nutritional and bioactive properties. In addition, the effects of the cereal bran cell wall (CBCW) on functional and rheological properties of wheat flour were elucidated. For this purpose, the cell wall was extracted from different cereal bran (maize, oat, and wheat) via an enzymatic method and characterized for its monosaccharides and phenolic acids contents. Additionally, the rheological properties of wheat flour with the addition of CBCW were determined using the farinograph and mixograph. The results showed that the cell wall contents in oat bran (43.31 ± 1.41%) were higher than those in wheat (41.53 ± 0.09%) and maize bran cell walls (37.31 ± 1.05%). The maize bran cell wall has higher arabinose, xylose, galactose, and glucuronic acid contents, followed by oat and wheat bran cell walls. Furthermore, the oat bran cell wall contained higher total phenolic acid contents as compared to maize and wheat bran. Nevertheless, the ferulic acid content was high in the maize bran cell wall. Moreover, incorporation (2%) of CBCW significantly improved the rheological properties of wheat flour. The water absorption (66.04 ± 0.23%), dough stability (4.54 ± 0.6 min), peak height (66.02 ± 0.03BU), and mixing tolerance index (70.02 ± 0.07%) were high in wheat flour with the addition of oat bran cell walls followed by wheat and maize bran cell walls. Furthermore, the dough development time (6.75 ± 0.25 min) was the highest in the control sample and the lowest mixing time was observed in C4 (5.41 ± 0.12 min), while the softness of dough (139.4 ± 0.02%) was decreased more significantly through the oat cell wall than wheat and maize. Conclusively, CBCW contained different bioactive moieties that have numerous positive effects on the rheological and functional properties of wheat flour. [ABSTRACT FROM AUTHOR]

In the present study, four different variants, namely Gp‐1, Gp‐2, Gp‐3, and Gp‐4, were characterized for their nutritional and fatty acid profile. Later on, the nutritionally superior variant was used for bread preparation. Purposely, composite flour was prepared with different ratios of wheat and rye (100:0; 90:10; 80:20). Furthermore, structural characterization of bread was done using Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM). Results showed that the Gp‐2 was more nutritional among the four variants. Furthermore, the spectra of composite flour bread were scanned in the range of 4000–600 cm–1. All the bread samples presented almost similar spectra for major peaks corresponding to wavenumbers in the functional group. The SEM micrographs showed the presence of small and large starch particles with compact structures. Conclusively, rye flour supplementation has a significant impact on the nutritional and structural attributes of the bread. [ABSTRACT FROM AUTHOR]

Soy isoflavones (SI) have strong estrogenic effect in tissues by binding to estrogen receptors and might be beneficial for women with polycystic ovarian syndrome (PCOS) by reduction in testosterone, cholesterol, insulin, weight gain, inflammatory markers, and oxidative stress. The study was planned to examine the effect of various levels of SI on nutrient intake, digestibility, lipid profile, insulin, and reproductive hormones of estradiol valerate (4 mg/rat/IM) PCOS induced rat models. Thirty‐six Wistar 45 days old rats weighing 95 ± 5 g were divided into 4 groups, each having 9 rats: C (control: without SF), SF10 (SF 0.10 g/kg BW), SF15 (SF 0.15 g/kg BW), and SF20 (SF 0.20 g/kg BW). SF was given through the oral gavage. Food and water were offered ad libitum, and intake was recorded daily. During last week of trial, collected feces by total collection method and blood samples were used to calculate nutrient digestibility and biochemical analysis, respectively. Estrogen, progesterone, and prolactin were (p <.05) high in rats fed SF diet that was 4% and 30% increase from C, respectively. Insulin, testosterone, FSH, and LH were lowest in rats fed diet SF20. Significant (p <.05) reduction in cholesterol was observed in rats fed SF15 and SF20 as compared to C. Serum HDL was improved (p <.05) in all SIF0.10, SIF0.15, and SIF0.20 in comparison with PC. Serum LDL was significantly reduced to 68.89 ± 4.36 (mg/dl) in SIF0.20 and to 108.20 ± 4.14 (mg/dl) in SIF0.15, whereas insignificant reduction was observed in SIF0.10 as compared to PC. Highly significant reduction was noted in triglycerides level in SIF0.20; however, significant reduction was observed in both SIF0.10 and SIF0.15 as compared to PC after 3 months of treatment with soy isoflavones. The objective of this study was to investigate the effect of different levels of soy isoflavones on blood insulin, lipid, and reproductive hormones profile of the female rats with PCOS. [ABSTRACT FROM AUTHOR]

Scientific literature is evident that the germinated seeds possess a promising potential for essential nutrients, flavors, and textural attributes over nongerminated grain. In recent decades, sprouting has also been investigated as a potential green food engineering technique to boost the nutritive profile of grains. Sprouting grains have multifold applications in different fields such as baking, pharmaceutical, and cosmetic industries. During sprouting, shifting of molecular structures to macroscopic takes place. Sprouting reactivates the grain metabolism which leads to the catabolism and degradation of antinutrient and macronutrient compounds. These modifications have an effect on human health and on the nutritional content of the foodstuffs. Sprouting grains have high bioactivity against diabetes and cancer. Germination is also an outstanding green food development technique to increase the seed nutritive profile in terms of quality. The present review focuses on the sprouting of grains, changes in nutritional profile, and the technological exploration of sprouted grains. [ABSTRACT FROM AUTHOR]

Abstract This study was intended to explore the effect of extruded flaxseed meal supplemented diet on broiler growth performance, oxidative stability and organoleptic characteristics of broiler meat and meat products. 120 (day old) broiler chicks were randomly allotted to 12 experimental groups and fed on diets containing extruded flaxseed meal at 0, 5, 10 and 15%. The supplementation of extruded flaxseed in the diet decreases the body weight gain, feed intake and increased feed conversion ratio (FCR) values of broilers. The antioxidant enzymes were strongly influenced by different levels of extruded flaxseed supplementation among treatments. The TBARS assay revealed that maximum malondialdehyde were produced in T3 containing highest extruded flaxseed level (15%) and minimum malondialdehyde were produced in T0 treatment having no extruded flaxseed. The TBARS values ranged from 0.850-2.106 and 0.460-1.052 in leg and breast met respectively. The Free radical scavenging activity varied significantly and DPPH values of breast meat ranged from 20.70% to 39.09% and in leg meat 23.53% to 43.09% respectively. The sensory acceptability of broiler meat nuggets was decreased with the increase in the level of flaxseeds due to the lipid peroxidation of polyunsaturated fatty acids (PUFA) which generated off flavors and bad odors. Feeding extruded flaxseed to chicken through feed strongly inflated the quality and functional properties, fatty acid contents and reduced the oxidative stability of broiler meat and meat products. The present study concludes that up to 10% of flaxseed meal may be used in broiler diet to enhance the omega 3 fatty acids content in the broiler meat.

The present study was aimed to evaluate the effects of wheat germ oil and wheat bran fiber as fat replacers on quality and stability of low‐fat beef patties. Total five treatments were prepared by employing wheat germ oil (WGO) and wheat bran fiber (WBF). WBF was used at fixed amount of 3% in all treatments except control in conjunction with varying WGO concentrations as follows: 1.5%, 3%, and 4.5%. Prepared raw and cooked beef patties were stored at 4°C, and further analyses were carried out up to 21 days of storage period with intermittent evaluation interval of 7 days. Higher values of TBARS, peroxide, and cholesterol were observed in raw and cooked beef patties in control, whereas minimum values were found in treatment of beef patties prepared with WGO 4.5% + WBF 3%. The physicochemical parameters were observed by pH and hunter color values. pH was higher in cooked patties as compared to beef patties and showed increases with increase in WGO concentration and storage intervals. The sensorial attributes were observed which included different parameters, such as appearance, texture, taste, odor, and overall acceptability. Higher score was given by the panelists to control for both raw and cooked beef patties; however, minimum score for all sensory properties was found in group treated with WGO 4.5% + WBF 3% within acceptable limit. In nutshell, raw and cooked beef patties treated with WGO 4.5% plus WBF 3% showed better quality, stability, and reduced cholesterol content. [ABSTRACT FROM AUTHOR]

The current research was designed to determine the amino acid profile and microbial properties of kachra hareer. For the purpose, kachra hareer was purchased with their brand names coded as S1, S2, S3, and S4 from five towns named as L1, L2, L3, L4, and L5. The samples were analyzed for their amino acid profile and microbial properties, while the water and alcohol extractive values were analyzed to obtain the different levels of purity during processing. The results explained that physicochemical analyses, that is, pH, moisture, acidity, and vitamin C, ranged from 4.67% to 9.44%, 22% to 40%, 0.054% to 1.44%, and 7.67% to 19.36%, respectively. However, essential amino acids histidine, isoleucine, leucine, methionine, phenylalanine, proline, threonine, and valine ranged from 470.3 to 484.5, 516.1 to 527.5, 805.5 to 817.4, 910.1 to 922.5, 240.2 to 250.5, 508.2 to 518.2, 1,160.5 to 1,172.6, 466.9 to 476.3, and 502.5 to 513.4 mg/kg, respectively, whereas nonessential amino acids alanine, arginine, aspartic acid cysteine, glutamic acid, serine, tyrosine, and glycine ranged from 612.7 to 628.2, 1,212.3 to 1,225.9, 7,254.2 to 7,258.8, ND, 1,561.2 to 1,575.2, 634.8 to 648.9, 618.4 to 630.4, and 712.8 to 723.7 mg/kg, respectively. Conclusively, sample S2 showed the maximum nutritional content and minimum microbial load. [ABSTRACT FROM AUTHOR]

Encapsulation technology is gaining attention across the world owing to its promising protection of active ingredients under hostile conditions. Various wall materials are used in the encapsulation of these sensitive ingredients. However, the legume proteins (LPs) are emerging and unique carriers for the delivery of bioactive owed to their biocompatibility, film formation and functional attributes. Legume proteins loaded with active ingredients can be used for the development of various functional foods. Modification strategies are making the legume proteins effective wall materials against various hostile conditions for the protection of probiotics and other sensitive ingredients. The present review describes the promising potential of legumes for the protection of active ingredients. Additionally, the effect of various modification processes on the functional properties of legumes has been reviewed. [ABSTRACT FROM AUTHOR]

Moringa oleifera, also called zogale, is grown and harvested as a tiny and middle-sized tree in different parts of the world. It has various industrial applications, such as medicine, food, and cosmetics. M. oleifera tree is known as a miracle tree containing essential nutrients that could play a significant role in human nutrition. Based on its chemical composition and nutritional properties, it can be used to overcome malnutrition, particularly in breastfeeding mothers and infants. Different parts (leaves, peels, seeds, and flowers) of the Moringa tree are good sources of phenolic compounds, essential amino acids, vitamins, proteins, and beta carotenes, which can play a regulatory and functional role. The proportion of bioactive components is dependent on the source and variety of the plant. The flowers, seeds, and leaves of this plant have also been used in different food applications. This review highlights the reported knowledge relevant to the use of M. oleifera as a food fortificant and therapeutic agent based on its prominent biological activities and the presence of phytochemicals. In a nut shell, the leaves, peels, seeds, and flowers of the Moringa tree are potential sources of different phenolic compounds, which act as anti-inflammatory, antidiabetic, neuro-enhancer, and anticancer. There is a dire need for planned and well-structured experimental designs to deliver possible future applications of this tree. [ABSTRACT FROM AUTHOR]

The current review focused on the effect of different thermal and non-thermal processing techniques on the structural and functional modifications of milk proteins. In thermal processing, denaturation in whey proteins takes place at the temperature range of 60–100°C. High temperature short time (HTST) treatment caused denaturation with the loss of secondary structure of proteins at temperature of 72°C for 15 minutes. Ultra-high temperature (UHT) treatment damaged β-lactoglobulins at higher temperature range of 135–140°C for 2 seconds. High-pressure processing (HP) (≤200-400 MPa) caused denaturation and aggregation of casein micelles with large molecular size. High-pressure homogenization with 350–400 MPa caused modification in structure of milk proteins and enhanced the solubility of whey proteins. Gamma irradiation with the dose of 3 kGy and 10 kGy crosslinked bands from α-lactalbumin and β-lactoglobulins respectively. At the dose of 32–64 kGy, crosslinking among caseinates appeared and molecular size of whey proteins increased. Dose of ultraviolet (UV) irradiation (254 nm) caused stability and improvement in the structure of whey proteins. Sonication treatment (20 W for 60 minutes) caused reduction in the size of casein micelles up to 1–4 nm. [ABSTRACT FROM AUTHOR]

In most of the countries around the world, the ever increase in food processing industries around the world is producing various types of by-products including peel, bran, pomace, refused pulp, shells, hull, husk, pods, seeds. With increasing activity in health-promoting functional foods, increase in the demand for natural bioactive moieties and new areas of research are on the way. Many by-products have been explored for health endorsing properties however, maize bran cell walls (MBCWs) and their various components (non-starch polysaccharides) are of chief importance. In this review, efforts are focused to illustrate the functional and nutraceutical importance of maize bran cell walls. Maize bran cell wall is made up of non-starch polysaccharide materials including arabinoxylans, heteroxylans, and hemicellulose. It holds various bioactive compounds such as ferulic acid, diferulic acid, and p-coumaric acid, having enormous nutraceutical properties, like antioxidant, anticancer, anti-inflammatory, anti-microbial, antiaging, cardioprotective, prebiotic and antiviral effects. These bioactive compounds are protecting against various disorders, like hypercholesterolemia, diabetes, cancer, neurodegenerative, cardiovascular, and thrombosis diseases. Furthermore, extraction, functional and nutraceutical properties, possible uses, and industrial applications of MBCW are the limelight of this article. [ABSTRACT FROM AUTHOR]

The main objective of current study was to evaluate the antioxidant potential and nutritional composition of milk thistle with special reference to silymarin. For the purpose, different varieties of milk thistle were procured from three different cities of Pakistan. The study was comprised of three different phases. In 1st phase, nutritional composition, that is, moisture, fat, protein, fiber, and nitrogen free extract, was determined according to their respective methods. Moreover, antioxidant potential and quantification of silymarin content were explored in 2nd phase. Furthermore, in last phase, milk thistle seeds tea was developed and evaluated for nutritional and sensorial characteristics. At last, data obtained from each parameter was subjected to appropriate statistical design to determine the level of significance. Results showed significant difference in the nutritional and chemical composition of different milk thistle varieties as well as locations. Moreover, moisture content, ash content, fat content, fiber content, protein content, and NFE varied from 6.27% to 5.01%, 2.37 to 1.25%, 23.19 to 19.74%, 7.4 to 4.39%, 30.09 to 20.74%, and 45.42 to 34.13%, respectively. Furthermore, silymarin content quantified though HPLC ranged from 1669.5 mg/g to 1607.6 mg/g for soxhlet extract whereas, 1,840.6 mg/g to 1765.9 mg/g for microwave‐assisted extraction extract. Conclusively, it was depicted from the results that in case of variety, Blue was the best than White whereas, Islamabad was best in case of location. [ABSTRACT FROM AUTHOR]

Child malnutrition is one of the biggest problems in developing countries with higher level of food insecurity. Pakistan is 5th largest producer of date fruit; therefore, its processing and products should be explored in various dimensions. Being rich source of minerals and sugars, it can contribute in weaning foods in a good manner. In current study, three blends were prepared with specific proportions of spray‐dried date powder and rich in specific proportions. They were compared with each other and control (free of date powder). The nutritional profile of the formulations revealed that 100 g of each formulation included all macronutrients in compliance with the requirements of Food and Agriculture Organization for weaning formulations; moreover, they are enriched with minerals due to presence of date powder. The iron contents reached up to 12.74 ± 0.16 mg/100 g. The phosphorus, zinc, and potassium contents also increased with the increase in date powder subsequently. Physicochemical properties exhibited in compliance with the requirement of the weaning foods. The protein quality was assessed both in vivo and in vitro. Amino acid profiling indicated that the limiting amino acid in F1 and control were lysine but in F2 and F3 were threonine. This is suggested that date powder might contain good quality protein that was further explained in biological studies, the formulations that contained higher amount of date powder reveled better PDCAAS score 86.76 ± 4.5, true digestibility 84 ± 4.36, biological value 69.45 ± 0.69, net protein utilization 73.82 ± 1.46, and protein efficiency score 1.18 ± 0.07. The sensory evaluation revealed that F2 showed better result in overall acceptability. Thus, date powder is suggested to be used as good constituent that can fortify mineral contents and sugar contents of the weaning foods. [ABSTRACT FROM AUTHOR]

The study was aimed on nutritional comparison of different four varieties of date fruit including (Ajwa, Hallawi, Madjool and Shamran), exploration of their functional ingredients i.e. antioxidant profile (total phenolic contents) and dietary fiber with special reference to the hypo-cholesterolemic capacity of the functional ingredients. Considering the aforementioned essentials, all the four date varieties were analyzed comparatively. The results showed significant difference in moisture content, ash contents, protein value, fat contents, fiber contents and nitrogen free extract (NFE) contents of different date varieties (p<0.05). Maximum ash contents (3.23%), crude protein (2.89%) and crude fat (0.49%) contents were recorded in Ajwa while it showed a minimum content of NFE (91.36%) as compared to the other date varieties. Maximum moisture content (23.12%) was found in Hallawi. Hallawi showed minimum contents of Crude fiber (1.88%) while Madjool showed highest contents of crude fiber (2.41%). Mineral estimation showed that potassium was the predominant mineral and ranged from (480.3 to 852.15 mg/100g) in Ajwa and Hallawi, respectively. Total phenolic contents ranged from 238.89 mg/100 (Madjool) to 280.67 mg/100g (Hallawi) while the highest DPPH scavenging activity was observed in Madjool followed by Ajwa (72.15%), Hallawi (69.17) and the lowest was found in Shamran (61.18%).Total flavonoid contents ranged from 96.05 mg/100 (Shamran) to 172.07 mg/100g (Ajwa) while the highest total flavonoid activity was observed in Ajwa (172.07 mg/100g) followed by Hallawi (157.07 mg/100g) and the lowest was observed in Shamran (96.05 mg/100g). Maximum content of Total dietary fiber (11.02%) and soluble dietary fiber (3.0%) was observed in Hallawi while in Ajwa (7.25%), Hallawi (8.02%) and Shamran (7.92%) showed a significantly (p= 0.05) higher insoluble dietary fiber in comparison with Madjool (4.30%). In addition, the date variety was tested in the experimental mouse model to evaluate its maximum cholesterol lowering effect. It was observed that Ajwa and Hallawi could effectively inhibit cholesterol level, which was 110 mg / dl and 103 mg / dl, respectively. According to the above chemical characteristics and parameters, the results of Ajwa and Hallawi dates were almost the same, and the greatest potential of lowering cholesterol, LDL and TG in serum was found. [ABSTRACT FROM AUTHOR]

The current study was aimed to evaluate the effects of guava and papaya leaves extract on the antioxidant profile and their outcomes in the storage stability of shrimp patties. Total of seven treatments were prepared by employing guava leaf extract (GLE) and papaya leaf extract (PLE) including control. Both the extracts were employed in the concentration of 1% and 2% each and in combination as 1:1% and 2:2%, respectively. The shrimp patties were kept in ziplock bags at refrigeration temperature, and further analysis was done after 21 days of storage period with intermittent evaluation interval of 7 days. The antioxidant capability of functional shrimp patties was determined by total phenolic content (TPC), 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH), and ferric reducing antioxidant power (FRAP). Higher significant values of TPC, DPPH, and FRAP were observed in the functional shrimp patties enriched with GLE2%:PLE2% at start of the experiment. The physicochemical parameters were observed by hunter color, TVBN, TBARS, and peroxide value (POV). Higher significant values of TVBN, TBARS, and peroxide were observed in the control samples as compared to treatment group GLE2%:PLE2%. The bacterial counts were also higher in control samples as compared to the treatment group GLE2%:PLE2%. The sensorial attributes were observed regarding appearance, taste, texture, odor, and overall acceptability. The maximum scores related all parameters were gathered by control group but significantly lower scores were for the group GLE2%:PLE2%. In conclusion, functional shrimp patties enriched with GLE2%:PLE2% showed better antioxidant capacity, stability, and sensory characteristics during storage. [ABSTRACT FROM AUTHOR]

The study designed to evaluate the role of sesame, fenugreek flax, and nuts seeds as best alternatives for therapeutic agents to improve bone mineral status. Effect of these plant seeds on proximate composition, antioxidant potential, mineral, fatty acid, and amino acid profile of supplements was studied. The sesame, fenugreek, and flaxseeds were incorporated with nuts at level of 35% to develop supplement. Improved contents of protein, fat, and NFE were recorded in range of 25.72–32.23, 18.92–23.15, and 34.25%–39.59%, correspondingly. The antioxidant potential of supplements is high (450.56 mg GAE/ 100g) prepared with fenugreek followed by flaxseeds and sesame seeds. Calcium as fundamental bone mineral found greater (525.15%–790.21%) in seed‐based supplement. Enhanced contents of Oleic, linoleic, and linolenic acids ranged 17.32–41.78, 21.72–44.23, and 1.00%–47.12%, respectively. Nutritional enriched supplements may be best alternatives for improving bone health by lowering osteoclast and increasing osteoblast mechanism in bone development. [ABSTRACT FROM AUTHOR]

The current study was conducted to elucidate the impact of encapsulation on the stability and viability of probiotic bacteria (Bifidobacterium bifidum) in cheddar cheese and in vitro gastrointestinal conditions. Purposely, probiotics were encapsulated in two hydrogel materials (kepa carrageenan and sodium alginate) by using an internal gelation method. Cheddar cheese was supplemented with unencapsulated/free and encapsulated probiotics. The product was subjected to physicochemical (pH, titrable acidity, moisture, and protein) and microbiological analysis for a period of 35 days of storage. Furthermore, the probiotics (free and encapsulated) were subjected to simulated gastrointestinal conditions. The initial probiotic count in cheese containing encapsulated probiotic was 9.13 log CFU/g and 9.15 log CFU/g which decreased to 8.10 log CFU/g and 7.67 log CFU/g while cheese containing unencapsulated probiotic initially 9.18 log CFU/g decreased to 6.58 log CFU/g over a period of 35 days of storage. The incorporation of unencapsulated and encapsulated probiotic affected the physicochemical, microbiological, and sensory attributes of the cheese. The encapsulated probiotic bacteria exhibited better survival as compared to unencapsulated probiotic. A 2.60 CFU/g log reduction in unencapsulated cells while just 1.03 CFU/g and 1.48 CFU/g log reduction in case of sodium alginate and K‐carrageenan, respectively, was recorded. In short, encapsulation showed protection and stability to probiotic in hostile conditions. [ABSTRACT FROM AUTHOR]

The present study was designed to assess the incidence of aflatoxin contamination in animal feed and raw milk samples (total 240 each) collected from dairy farms during the complete year of 2015. These samples were collected through a cluster random sampling technique by dividing the province of Punjab, Pakistan into five clusters (north, south, east, west and central). Factors (environmental & physical) affecting aflatoxin contamination in milk and animal feed at farms were also studied. The AFM1 levels in raw milk & AFB1 levels in feed samples were analyzed by using the ELISA technique. Results demonstrated that overall about 53% raw milk samples from dairy farms were contaminated beyond the US MRL (0.50 µg/L) for AFM1 with than average level of 0.59 µg/L, while the 95% farm feed samples were exceeding the FDA MRL (20 µg/kg) of AFB1 with average level of 43 µg/kg. During winter season, the concentration of AFM1 was higher in all clusters with avg 0.68 µg/L, while the AFB1 contamination was highest in the spring season with avg 54 µg/kg. Market feed prices had negative correlation with AFB1 contamilevels, which were further supported by the positive correlation between quantity of feed at farms with AFM1 and AFB1 contamination. Results exhibited significantly positive impact of environmental factors on milk and feed aflatoxin contamination levels, whereas temperature showed an inverse relationship with AFM1 and AFB1 levels. The study recommends need of synergistic extension work to support dairy farms and highlight the contamination levels for regulatory bodies to introduce strategic policies for control measures. [ABSTRACT FROM AUTHOR]

The present study was designed with the objective to compare the viability and stability of free and encapsulated probiotics under simulated technological and human gastrointestinal conditions. L. acidophilus was encapsulated using two wall materials (sodium alginate, soy protein isolate, and SA‐SPI) by extrusion method for enhanced viability under stressed conditions. Free and encapsulated probiotics were subjected to some simulated technological and gastrointestinal conditions. Furthermore, free and encapsulated probiotics were also incorporated in dairy dessert to evaluate the viability and stability during storage. Encapsulation using sodium alginate and SPI as a coating materials significantly (p <.05) improved the survival of probiotics under simulated gastrointestinal and thermal conditions. The buffering effect of microbeads prolonged their survival and stability of under simulated conditions. The number of surviving probiotic cells encapsulated with sodium alginate, SPI, and SA‐SPI over 120 days of product storage was 7.85 ± 0.39, 7.45 ± 0.37, and 8.50 ± 0.43 cfu/ml, respectively. In case of free cells, the surviving cells were just 3.5 ± 0.18 cfu/ml over the period of storage. In short, the study depicted that encapsulation provides protection during exposure to various hostile conditions. [ABSTRACT FROM AUTHOR]

The aim of the present study was to evaluate the upshot of microencapsulation on the stability and viability of probiotics in carrier food (ice cream) and simulated gastrointestinal (GIT) conditions. Purposely, Lactobacillus casei was encapsulated with two different hydrocolloids, that is, calcium alginate (Ca‐ALG) and whey protein concentrate (WPC) by using encapsulator. The obtained microbeads were characterized in terms of encapsulation efficiency and morphological features. Afterward, the probiotics in free and encapsulated form were incorporated into ice cream. The product was subjected for physicochemical, microbiological, and sensory attributes over a storage period of 80 days. Microencapsulation with both hydrogels significantly (p <.05) improved the viability of probiotics in both carrier food and simulated GIT conditions.The initial viable count of probiotics encapsulated with Ca‐ALG and WPC was 9.54 and 9.52 log CFU/ml, respectively, that declined to 8.59 and 8.39 log CFU/ml, respectively, over period of 80 days of storage. While nonencapsulated/free cells declined from 9.44 to 6.41 log CFU/ml during same storage period. Likewise, during in vitro GIT assay, encapsulated probiotic with Ca‐ALG and WPC showed 0.95 and 1.13 log reduction, respectively. On other hand, free probiotics showed significant 3.03 log reduction. Overall, microencapsulated probiotic exhibited better survival as compared to free cells. Moreover, the amalgamation of encapsulated and free probiotics affected the physicochemical (decrease in pH and increase in viscosity) was and sensory parameters of ice cream during storage. [ABSTRACT FROM AUTHOR]

The core objectives of the current study were to explore and compare the proximate and nutritive composition of both types of cookies prepared either with Ajwa seed oil (ASO) or hydrogenated vegetable fat (HVF) and comparative analysis of the antioxidantal potential of both cookies and to reconnoiter the storage effects on the quality parameters of the cookies over a period of 60 days. Outcomes revealed that the cookies prepared with the ASO were superior (p <.05) in fat% and energy as compared to the cookies with HVF. Due to the presence of functional moieties, ASO cookies were found with better palatability, increased shelf life with enhanced physical properties and better functionality. Therefore, the findings of the current study revealed that ASO could be used as an alternative fat source for the preparation of novel functional cookies instead of conventional vegetable fats. [ABSTRACT FROM AUTHOR]

The core objective of the current study was to assess the effect of encapsulation on the viability and stability of probiotic bacteria under simulated gastrointestinal digestion and thermal conditions. Purposely, probiotics were encapsulated with hydrogel matrices (sodium alginate and carrageenan) using encapsulator. Furthermore, developed microbeads were characterized by X-ray diffraction (XRD) and Fourier Transform Infrared (FTIR) spectroscopy to elucidate the interaction between hydrogel matrices and probiotics. The viability of probiotics assessed by subjecting to simulated GIT and thermal conditions. Encapsulation exhibited a significant (p< .05) effect on the survival and stability of probiotics. Overall, a decreasing trend in viability of probiotics was observed in all treatments. A rapid log reduction was observed when free probiotic cells were stored at refrigeration temperature compared to encapsulated probiotic bacteria. Likewise, in vitro gastrointestinal assay, only 3 log while in case of non-encapsulated bacteria 6 log reduction was recorded. In short, the results of the viable count in the case of encapsulated cells were above recommended level (106 cfu/g) under thermal as well as in GIT conditions. [ABSTRACT FROM AUTHOR]

The aim of the present study was to prepare designer yogurt for the hypercholesterolemic subject. The yogurts were prepared from sheep and cow milk by fortifying omega fatty acids and dietary fibers of extruded flaxseed powder (EFSP). The EFSP, Streptococcus thermophilus, and Lactobacillus delbrueckii sub sp bulgaricus at 2% were added for yogurt formation. The water retention capacity, fat adsorption capacity, swelling, and solubility of EFSP were 14.38 ± 3.16 g/g, 5.31 ± 0.93 g oil/g, 25.57 ± 1.35 ml/g, and 30.53 ± 8.5%, respectively. The proximate value of protein content increased significantly from 4.12% to 5.12%. Total fat content increased from 3.50% to 4.28%. Total dietary fibers increased from 0% to 24.14%. Significant increase was observed in C18:1n9 and C18:2n6 fatty acid contents. The omega‐3 (18:3n3) was significantly increased higher as compared to other omega fatty acids in fortified yogurts. Atherogenicity index (IA: 2.23 ± 0.41 to 1.42 ± 0.23) and thrombogenicity index (IT: 1.68 ± 0.95 to 0.65 ± 0.01) were significantly decreased while the ratio of hypocholesterolemic and hypercholesteremic fatty acids (HH: 0.82 ± 0.05 to 1.29 ± 0.46) increased significantly in sheep milk yogurt. IA (2.74 ± 0.31 to 1.48 ± 0.08) and IT (1.84 ± 0.28 to 0.39 ± 0.04) were also decreased significantly while HH (0.54 ± 0.05to 1.12 ± 0.02) increased significantly in cow milk yogurt. Δ9‐desaturase (18) index was found highest (75.67 ± 8.04) in EFSP‐fortified sheep milk yogurt and lowest (62.27 ± 8.65) in cow milk yogurt while Δ9‐desaturase (16) index was maximum (6.21 ± 1.25) in cow milk yogurt and minimum (3.93 ± 1.38) in EFSP‐fortified sheep milk yogurt. Significant effect on consistency, firmness, viscosity index, and cohesiveness was also noticed in fortified yogurts. EFSP has also significant impact on colony formation of stains culture. The fortified product got good flavor and textural acceptance score. Thus; EFSP improved the health lipids quality and physicochemical characteristics. It can act as a good stabilizer and texturizer in yogurt formation and a healthy alternative for hazardous adulterants used in yogurt formation. [ABSTRACT FROM AUTHOR]

The core objective of the current study was to evaluate the effect of microencapsulation on the viability and stability of probiotic bacteria in yogurt and simulated gastrointestinal conditions. For this purpose, probiotic bacteria were encapsulated with sodium alginate and carrageenan by encapsulator. Yogurt was prepared with the incorporation of free and encapsulated probiotic bacteria and was analyzed for physicochemical, microbiological, and sensorial attributes. Encapsulation and storage exhibited a significant (p <.05) effect on different parameters of yogurt. An increasing trend in syneresis and acidity while a decreasing trend in viscosity, pH, viability, and stability were observed. The value of syneresis increased from 2.27 ± 0.17 to 2.9 ± 0.14 and acidity from 0.48 ± 0.04 to 0.64 ± 0.01 during 4 weeks of storage. The value of viscosity decreased from 3.68 ± 0.21 to 2.42 ± 0.09 and pH from 4.88 ± 0.31to 4.43 ± 0.36 during 28 days of storage. Unencapsulated (free) cells exhibited poor survival. The viable cell count of probiotic bacteria in the free‐state in yogurt was 9.97 logs CFU/ml at zero‐day that decreased to 6.12 log CFU/ml after 28 days. However, encapsulation improved the viability of the probiotics in the prepared yogurt and GIT. The cell count of probiotics encapsulated with sodium alginate and carrageenan was 9.91 logs CFU/ml and 9.89 logs CFU/ml, respectively, at zero‐day that decreased to 8.74 logs CFU/ml and 8.39 log CFU/ml, respectively. Free cells (unencapsulated) showed very poor survival. Similarly, during in vitro gastrointestinal assay, the survival rate of encapsulated probiotic bacteria in simulated gastric solution and intestinal solutions was higher than that of free cells. In the case of encapsulated bacteria, only 3 logs while for free cells, 7 log reduction was recorded. Sodium alginate microcapsules exhibited better release profile than carrageenan. Conclusively, microencapsulation improved the survival of probiotic bacteria in carrier food as well as in simulated gastrointestinal condition. [ABSTRACT FROM AUTHOR]

Muskmelon (Cucumis melo) fruit is a best source of vitamins, minerals, and bioactive components. Ingestion of high sugary drinks leads to numerous ailments such as diabetes mellitus, obesity, and tooth decay. This study intended at formulation of intermediate moisture food with various combinations of sugar and glycerol and same levels of potassium metabisulphite, potassium sorbate, calcium chloride, and citric acid. It was observed a gradual decrease in TSS (56.53–53.28), vitamin C level in all treatments with the passage of time. The declining trend in ascorbic acid (25.49–21.63 mg/100 g) content of muskmelon chunks was increased as a function of storage. Sensory results showed that there was declining trend in the scores obtained for color parameter, that is, L* from 60.23 to 55.98. The overall results showed that combination of different additives contributed best values (T3) for flavor (7.70), taste (8.15), vitamin C (25.60 mg/100 g), and pH (5.16) as compared to other treatments. Conclusively, developed chunks of treatment 3 are physicochemical and organoleptically considered best, as it is helpful to sustain life stability of muskmelon and enhance its marketability. [ABSTRACT FROM AUTHOR]

Dietary fiber has gained greater attention owing to their positive and potential health perspectives. Cereals are the most important and enriched source of dietary fiber with more insoluble dietary fiber than soluble. For dietary fiber modification, chemical treatment with various techniques is considered as significant approach owing to its safety point of view and involves less damage to the molecular structure of the dietary fiber through chemical reagents and content of soluble dietary fiber is increased more efficiently. The current study was aimed to nutritionally characterize the cereal grains and to partially convert insoluble dietary fiber into soluble dietary fiber through chemical treatments in combination with extrusion. For the purpose, two varieties of each cereal were characterized for their chemical composition, mineral profile, and dietary fiber content according to the respective methods. Then, dietary fiber ratio in cereals was modified through chemical treatments, that is, acid, alkaline, and consecutive acid–alkaline followed by extrusion. Results regarding dietary fiber content of cereal grains exhibited that wheat (12.03–12.20 g/100 g) contained higher total dietary fiber followed by sorghum (6.70–6.90 g/100 g). Additionally, modification of SDF (1.97%) and IDF (11.48%) ratio in wheat and SDF (1.19%) and IDF (24.25%) ratio in sorghum through extrusion processing was nonsignificant while acid–alkaline treatment showed highly significant results, that is, 768.2% increase in SDF and 56.5% decrease in IDF in wheat and 952.38% increase in SDF and 71.17% decrease in IDF in sorghum. Among chemical treatments, higher result was given by acid–alkaline method and the lower outputs were observed in case of extrusion in both cereals. Conclusively, soluble dietary fiber was significantly increased through chemical treatments alone or in combination with twin‐screw extrusion. [ABSTRACT FROM AUTHOR]

The current research was carried out to observe the effect of different thermal treatments on soluble and insoluble dietary fiber ratio to improve functional properties of barley. Two varieties of barley labeled as Haider‐93 and Jau‐87 were milled and then wet and dry heat‐treated. Soaking and then cooking of soaked and nonsoaked barley was performed. Untreated barley contained more insoluble dietary fiber (12.00–12.40 g/100g dm) than soluble dietary fiber (4.73–5.70 g/100g dm). Additionally, the modification of soluble (13.32%) and insoluble dietary fiber (8.79%) ratio through pressure cooking was nonsignificant while roasting showed significant results, that is, 53.91% increase in soluble dietary fiber and 8.79% decrease in insoluble dietary fiber. In phase II, cooking without soaking gave highest results, that is, 68.08% increase in soluble dietary fiber and 15.48% decrease in insoluble dietary fiber. Conclusively, among all treatments of phase I and II, the better results were shown by cooking without soaking. [ABSTRACT FROM AUTHOR]

The aim of this present study was to explore antioxidant and bioactive profile of ginger and turmeric. For the purpose, turmeric and ginger (Haldi and Adric) were procured from University of Agriculture, Faisalabad, Punjab-Pakistan. The study was comprised of different phases. Both of the spices were characterized for their chemical composition and mineral profile. Bioactive compound was extracted by using solvent followed by quantification through the high-pressure liquid chromatography. Furthermore, antioxidant potential including total phenolics content, free radical scavenging activity (DPPH assay) and Ferric reducing antioxidant power test (FRAP assay) was analyzed. Results revealed that the antioxidant profile including free radical scavenging activity (47.67 ± 0.19 mg/100 g) and DPPH (80.16 ± 0.23%) of turmeric ginger powder extract was much higher than turmeric and ginger powder extract. Similarly, total phenolics content (103.39 ± 0.58 mg of GAE/g) and flavonoids (4.27 ± 0.05 mg CE/100 g) were much higher in turmeric ginger powder as compared to turmeric powder and ginger powder, respectively. Conclusively, turmeric ginger powder showed higher antioxidant potential. [ABSTRACT FROM AUTHOR]

Improved awareness regarding diet and health linkages has motivated researchers to give more attention to the health perspectives of individual foods. During the previous two decades, the global dairy industry diverted its interest from simple processing of commodities to the value addition. A significant income is being earned in the dairy industry from specialty proteins. Pakistan ranked 3rd in milk production worldwide and different milking animals e.g. cow, buffalo, goat and camel should be studied for their potential for generating new health-promoting diets and nutraceuticals foods. Bioactive proteins and peptides from having abundant applications including an antimicrobial food additive, dietary supplements, functional foods and nutraceuticals. Proteins from milk have a balanced composition of amino acids promising novel functional products. These also enhance the consistency and sensorial characteristics of various dairy products. Amongst, lactoferrin reveal bacteriostatic as well as bactericidal bustle against a variety of microorganisms. It binds iron thus depriving it required for growth like L. monocytogenes, Salmonella spp, Escherichia coli, Bacillus stearothermophilus, Shigella dysenteriae and Bacillus subtilis. Lactoferrin executes as an alternative to antibiotics. It acts as a natural antimicrobial for bio preservation ranging from dairy, meat, seafood, beverages, bakery products, acne care, infant formulas, extending shelf life, ensuring safety and improving health by acting against life-threatening diseases like cancer, hepatitis, respiratory infections, and foodborne diseases in infants, children and adults etc. In the nutshell, the current review discusses the importance and safety perspectives of lactoferrin in the food industry as well as the health sector. [ABSTRACT FROM AUTHOR]

The present study was conducted to evaluate the effects of storage time and temperature on physicochemical and sensory properties and to measure the spoilage of pasteurized fluid milk stored at different temperatures via nanoparticles on pasteurized fluid milk. The mean values for pH of milk samples decreased (from 6.81 to 6, 5.46, and 4.37), acidity increased (10%, 21%, and 35%), fat contents decreased (13%, 24%, and 32%), protein contents decreased (7%, 18%, and 24%), lactose contents decreased (5%, 7%, and 12%), and total free fatty acids increased (14%, 19%, and 27%) respectively at 4°C, 7°C, and 10°C. Similarly, the score of sensory parameters like aroma, taste, and overall acceptability decreased 16%, 23%, and 45%; 31%, 43%, and 52%; and 33%, 42%, and 53% respectively in linear pattern along with increasing the time and temperature. The L* values were non-significantly changed or decreased with mean values of 64.82 ± 0.08 to 64.13 ± 0.07 at 4°C and significantly with 64.81 ± 0.03 to 63.87 ± 0.07 and 64.77 ± 0.02 to 61.43 ± 0.05 at 7°C and 10°C respectively at 0–19th day of milk storage. During storage, the pasteurized milk stored at all temperatures 4°C, 7°C, and 10°C showed that a* value non-significantly decreased from 48.69 ± 0.18 to 48.63 ± 0.18, 48.74 ± 0.14 to 48.57 ± 0.11, and 48.68 ± 0.18 to 47.97 ± 0.07 respectively. Values of b* decreased non-significantly at 4°C and 7°C with the values of −17.56 ± 0.23 to −18.58 and −17.54 ± 0.25 to −19.85 ± 0.85 but significantly decreased at 10°C from −17.56 ± 0.23 to −43.22 ± 0.11. The results suggested that pasteurized milk can be kept for 17–19 days at 4°C but this shelf life drastically reduced at 7°C and 10°C. Color of nanoparticles was significantly changed from pink to dark blue from 14th day of storage onwards to the end at 10°C. Conclusively, nanoparticles can be used for the rapid and at the post-spoilage measurement of pasteurized milk. [ABSTRACT FROM AUTHOR]

This study was carried out to evaluate the impact of gamma irradiation and turmeric powder (TP) on microbial quality (total aerobic bacteria and coliforms), physicochemical quality (pH, Hunter's parameter, oxidative and microbial stabilities, haem pigment), stability, and antioxidant status of chicken meat. Accordingly, two doses (1 kGy and 2 kGy) of gamma irradiation alone and in combination with 3% TP along with the control (0 kGy) were applied. Aerobic and vacuum packaging were used for storage of chicken meat on the 0, 7th, and 14th days of storage at refrigeration temperature (4°C). The microbiological results showed that the contamination level decreased as the dose of gamma irradiation was increased for both total bacteria and coliforms, whereas no contamination was documented in the group treated with 2 kGy+TP for both aerobic and vacuum packaging. The results further showed that pH, haem pigment, and Hunter's colour were also significantly influenced with respect to different groups. The peroxide value (POV), thio-barbituric acid reactive substances (TBARS), and total volatile basic nitrogen (TVBN) differed significantly in chicken meat with different treatments and storage intervals. Higher POV and TBARS were noticed in chicken meat treated with 2 kGy under aerobic packaging after 14 days of storage, and TVBN was higher in the control on the 14th day under aerobic packaging. Total phenolics and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity were also higher in chicken meat treated with 2 kGy + TP on 0 day of storage. Furthermore, higher sensory attribute scores for attributes like appearance, taste, texture, flavour, and overall acceptability were found in the 2 kGy-treated group. It is concluded that chicken meat treated with 2 kGy+TP was considered better for microbial and physicochemical quality, antioxidant activity as well as sensorial properties of chicken meat. [ABSTRACT FROM AUTHOR]