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In this study, a rapid, precise, and targeted electroanalytical method was developed for the trace determination of 4-chlorophenol (4-CP). The study reports the use of cyclic voltammetry (CV) to characterize the electrochemical response of 4-CP and the optimization of differential pulse voltammetry (DPV) settings for its sensitive quantification. Screen-printed carbon electrodes (SPCEs) were selected for the sensitive detection of 4-CP using DPV. The incorporation of multi-walled carbon nanotubes functionalized with carboxyl groups (MWCNT-COOH) as a modifier on the working SPCE significantly enhances the electrode's performance, resulting in a 5-fold increase in sensitivity compared to that of the unmodified SPCE. Under optimal conditions, oxidation peak current exhibited a detection limit of 9.2 nM and was proportional to 4-CP concentration in the range of 0.01-1.3 μM. Additionally, the constructed sensor demonstrated high stability, high selectivity, good reproducibility, and excellent feasibility. These findings suggest that the C/MWCNT-COOH/SPE offers a simple, rapid, and cost-effective method for the prospective online assessment of 4-CP in various samples with different matrices.

Researchers are actively looking for novel anticancer medications because cancer is one of the leading causes of mortality worldwide. A fascinating area of study in medicinal chemistry is the screening of antioxidants for novel anticancer medicines, as antioxidants have lately been used as therapeutic candidates to combat a variety of ailments in aerobic species. Additionally, pyrazole-based heterocycle synthesis is a productive approach to the drug development process. To ascertain the molecular geometry and frontier orbital analysis, a DFT simulation of the produced compounds was conducted. Compound 7 showed the lowest energy gap and hardness, while compound 7 had the maximum softness. Therefore, a few quinazoline, benzimidazole, and tetrazinethione derivatives based on pyrazoles that were synthesized in our earlier work and exhibited antioxidant qualities were tested for their in vitro antiproliferative activity against the MCF7 and HCT116 cancer cell lines. The two cancer cell lines were most effectively inhibited by derivatives of sulfonamide and tetrazinethione. The molecular docking simulation toward CDK2 protein specified the best docking score of tetrazinethione 7 followed by sulfonamide derivative 4, compared to doxorubicin and roscovitine (kinase inhibitor). Most of the amino acids interacting with these compounds were involved in that interaction with the co-crystallized ligand. Their favorable oral bioavailability and drug-likeness characteristics were demonstrated by a modeling pharmacokinetics investigation. This research could help create novel antiproliferative drugs that are both efficient and selective., (© 2025 Wiley Periodicals LLC.)

A novel compact potentiometric electrode specifically designed for pH monitoring, featuring a good construction on a glass substrate coated with a cerium-doped tin oxide (Ce-doped SnO 2 ) layer. The Ce-doped SnO 2 thin film was created by spray-pyrolysis it on a glass substrate. Field emission scanning electron microscopy (FESEM) and X-ray diffraction (XRD) were used to characterize the deposited metal oxide coating. As a miniature potentiometric electrode, the synthesized Ce-doped SnO 2 /glass substrate was utilized to measure a broad pH range (pH 2-12) in aqueous solutions. The electrode had a perfect near-Nernstian response (slope of -58.6 ± 0.7 mV per decade), high potential stability, mechanical durability, and great selectivity towards some common interfering cations and anions. These characteristics made it ideal for quality control and assurance purposes. The electrode's performance parameters and validation measurements were assessed using established procedures. The Ce-doped SnO 2 -based electrode satisfactorily monitored the pH of several genuine water, drink, and fruit juice samples, and the data compared well with those obtained using a traditional pH glass electrode. The integration of Ce-doped SnO 2 as the active material marks a significant advancement, providing enhanced electrochemical stability, improved sensitivity, and a wider pH detection range compared to conventional electrodes. The compact design not only reduces the sensor's footprint but also facilitates its application in miniaturized and portable pH monitoring devices, making it highly suitable for advanced analytical and environmental sensing applications.

To remove organic and inorganic agrochemicals from contaminated soil and water, adsorption has been regarded as a viable remediation approach. For the removal of organic pollutants, such as pesticides, cost-effective adsorbents have garnered a lot of interest. These include waste-derived materials, clay composites, metal-organic frameworks (MOFs), nanocomposites, and biochar-modified materials. In this study, copper ferrite (CuFe 2 O 4 ) was prepared, characterized, and modified with aminated reduced graphene oxide (Am-rGO) to form a CuFe 2 O 4 /Am-rGO nanocomposite for the effective removal of imidacloprid (IMD) from water. The Langmuir isotherm model was used to determine the maximum adsorption capacity of the adsorbent (CuFe 2 O 4 /Am-rGO), which was estimated to be 13.1 (±1.5) mg g -1 . At 0.5 mg L -1 IMD, the adsorbents were able to extract up to 97.8% of the IMD from the aqueous solution. The Freundlich model and the pseudo second-order model agreed well with the experimental data, proving that physisorption and chemosorption both played a role in the sorption process. CuFe 2 O 4 /Am-rGO nanocomposite offers high stability and improved reusability due to its improved removal efficiency. After five adsorption-desorption cycles, there was no appreciable reduction in elimination. Additionally, after adsorption tests, IMD can be easily removed after adsorption by an external magnetic field. These showed that Am-rGO had changed the surface of CuFe 2 O 4 to make it easier for IMD to stick to it in aqueous solutions. When used adsorbent is co-processed with ethanol extraction and ultrasound cavitation, it can be regenerated and still work well as an adsorbent. Furthermore, CuFe 2 O 4 /Am-rGO demonstrated its environmental safety and ability to continue absorbing IMD across a variety of diverse matrices. As a result, this study demonstrates that CuFe 2 O 4 /Am-rGO is a long-lasting, easily prepared, and efficient adsorbent for the removal of IMD as one of the neonicotinoids., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (This journal is © The Royal Society of Chemistry.)

Environmental pollutants like chlorophenol chemicals and their derivatives are commonplace. These compounds serve as building blocks in the production of medicines, biocides, dyes, and agricultural chemicals. Chlorophenols enter the environment through several different pathways, including the breakdown of complex chlorinated hydrocarbons, industrial waste, herbicides, and insecticides. Chlorophenols are destroyed thermally and chemically, creating dangerous chemicals that pose a threat to public health. Water in particular is affected, and thorough monitoring is required to find this source of pollution because it can pose a major hazard to both human and environmental health. For the detection of chlorophenols, molecularly imprinted polymers (MIPs) have been incorporated into a variety of electrochemical sensing systems and assay formats. Due to their long-term chemical and physical stability as well as their simple and affordable synthesis process, MIPs have become intriguing synthetic alternatives over the past few decades. In this review, we concentrate on the commercial potential of the MIP technology. Additionally, we want to outline the most recent advancements in their incorporation into electrochemical sensors with a high commercial potential for detecting chlorophenols.

Because of the serious risks they pose to the environment and public health, chlorophenols (CPs), a typical class of the most persistent organic pollutants, have drawn increasing attention. Monitoring CPs effectively has become a pressing and difficult problem. The rapidly increasing need for onsite and real-time CP detection has led to the consideration of electrochemical sensing as a workable solution. Molecularly imprinted polymer (MIP)-based electrochemical sensing has emerged as a promising area for environmental monitoring in response to this analytical problem. MIPs, in conjunction with miniature electrochemical transducers, provide the opportunity to detect target analytes in situ . These devices have the advantages of great chemical and physical stability, cheap production costs, good selectivity, and quick response times. Most studies suggest that these sensors use nanoparticles to improve their analytical properties, especially sensitivity. Furthermore, these sensors have successfully used real water samples without the need for time-consuming pretreatment procedures. This article provides an overview of electrochemical MIP-based sensors reported to detect CPs in water samples. To obtain the highest sensitivity, special consideration is given to the fabrication of the sensors, which includes the use of various functional monomers, sensing platforms, and materials. Several other parameters are also discussed, including the linear concentration range, limit of detection, and the types of water samples that were examined., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (This journal is © The Royal Society of Chemistry.)

In this study, nickel selenide (NiSe), Ag/C 3 N 4 -NiSe, and C 3 N 4 /Ag-NiSe nanowires (NWs) were synthesized via coprecipitation. The prepared NWs were employed for the degradation of the rhodamine B (RhB) dye in the absence of light using sodium borohydride (NaBH 4 ), bactericidal activity against pathogenic Staphylococcus aureus ( S. aureus ) and in silico docking study to investigate the d-alanine ligase (DDl) and deoxyribonucleic acid (DNA) gyrase of S. aureus . NWs demonstrate a catalytic degradation efficiency of 69.58% toward RhB in a basic medium. The percentage efficacy of the synthesized materials was evaluated as 19.12-42.62% at low and 36.61-49.72% at high concentrations against pathogenic S. aureus . Molecular docking results suggest that both C 3 N 4 /Ag-doped NiSe and Ag/C 3 N 4 -doped NiSe possess inhibitory activities toward DDl and DNA gyrase of S. aureus , which coincides with the in vitro bactericidal activity. Based on the research outcomes, the synthesized NWs show potential as an effective agent for water purification and resistance to microbial contaminants., Competing Interests: The authors declare no conflict of interest., (This journal is © The Royal Society of Chemistry.)

Some hexahydroquinoline candidates were prepared by reacting 2-amino-3-cyano-1-cyclohexylhexahydroquinoline with oxalyl chloride and triethyl orthoformate. The computational chemical approach agreed with the product-testing results. The produced substances were examined in vitro for their antiproliferative activity against liver carcinoma (HepG2), breast adenocarcinoma (MCF7), prostate cancer (PC3), and colon cancer (HCT116) cell lines. The highest potency against the four cell lines was exhibited by hydrazide, thiosemicarbazide, and thiazolidinone derivatives. The best docking score was presented by thiosemicarbazide and thiazolidinone derivatives as they showed the highest binding to the Mcl-1 enzyme with binding energies of -8.97 and -8.90 kcal mol -1 , respectively, which were higher than that of the co-crystallized ligand (LC3) with a binding energy of -8.74 kcal mol -1 . Besides, the modeling pharmacokinetics disclosed their desirable drug-likeness and oral bioavailability characteristics., Competing Interests: The authors declare no conflicts of interest., (This journal is © The Royal Society of Chemistry.)

A paper-based analytical device (PAD) with an integrated composite electrode has been designed and fabricated for non-enzymatic creatinine sensing. Reduced graphene oxide (rGO) was employed to modify the PAD so that it could function as a solid-contact transducer. A new macrocyclic pyrido-hexapeptide derivative was made and used as a special ionophore in the creatinine membrane sensor. The synthesized PAD showed a detection limit of 1.0 µM (S/N = 3) and a potentiometric response towards creatinine throughout a log-linear range of 2.0 µM-10 mM (R 2  = 0.9998). The sensor shows significant selectivity for a few related substances, including ephedrine, codeine, ketamine, caffeine, urea, urate, carbinoxamine, and dextromethorphan. It has been established that the testing method is appropriate for the direct potentiometric detection of creatinine in a variety of human urine sample types. When an indicating electrode and a reference electrode are put on the same flexible disposable, this lets applications with a small sample volume be done. For point-of-care creatinine measurement, the developed paper-based analytical equipment is a good choice because it is affordable, easily accessible, and self-pumping (especially when combined with potentiometric detection)., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)

The harmful cationic dyes present in industrial waste significantly decrease the effectiveness of remedy operations. Considering the horrendous impact of these dyes on the environment and biodiversity, silver bromide (AgBr) and chitosan (CS) doped copper ferrite (CuFe 2 O 4 ) nanostructures (NSs) were prepared by the co-precipitation route. In this work, The surface characteristics of CuFe 2 O 4 can be altered by CS, potentially enhancing its catalytic reaction compatibility. The functional groups in CS interact with the surface of CuFe 2 O 4 , influencing its catalytic behavior. AgBr can have an impact on the dynamics of charge carriers in the composite. Better charge separation and transfer which is essential for catalytic processes. The catalytic degradation of RhB was significantly enhanced (100 %) using 4 wt% of AgBr-doped CS-CuFe 2 O 4 catalysts in a basic medium. The significant inhibitory zones (9.25 to 17.95 mm) inhibitory in maximum doses were seen against Gram-positive bacteria (S. aureus). The bactericidal action of AgBr/CS-doped CuFe 2 O 4 NSs against DNA gyrase S.aureus and tyrosyl-tRNAsynthetase S. aureus was rationalized using molecular docking studies, which supported their function as inhibitors., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier B.V.)

It is crucial for early stage medical diagnostics to identify disease biomarkers at ultralow concentrations. A wide range of analytes can be identified using low-dimensional materials to build highly sensitive, targeted, label-free, field-effect transistor (FET) biosensors. Two-dimensional (2D) materials are preferable for high-performance biosensing because of their dramatic change in resistivity upon analyte adsorption or biomarker detection, tunable electronic properties, high surface activities, adequate stability, and layer-dependent semiconducting properties. We give a succinct overview of interesting applications for protein sensing with various architectural styles, such as 2D transition metal dichalcogenides (TMDs)-based FETs that include carbon nanotubes (CNTs), graphene (Gr), reduced graphene oxide (rGr), 2D transition-metal carbides (MXene), and Gr/MXene heterostructures. Because it might enable individuals to perform better, this review will be an important contribution to the field of medical science. These achievements demonstrate point-of-care diagnostics' abilities to detect biomarkers at ultrahigh performance levels. A summary of the present opportunities and challenges appears in the conclusion.

Microplastics (5 μm) are a developing threat that contaminate every environmental compartment. The detection of these contaminants is undoubtedly an important topic of study because of their high potential to cause harm to ecosystems. For many years, scientists have been assiduously striving to surmount the obstacle of detection restrictions and minimize the likelihood of receiving results that are either false positives or false negatives. This study covers the current state of electrochemical sensing technology as well as its application as a low-cost analytical platform for the detection and characterization of novel contaminants. Examples of detection mechanisms, electrode modification procedures, device configuration, and performance are given to show how successful these approaches are for monitoring microplastics in the environment. Additionally included are the recent developments in nanoimpact techniques. Compared to electrochemical methods for microplastic remediation, the use of electrochemical sensors for microplastic detection has received very little attention. With an overview of microplastic electrochemical sensors, this review emphasizes the promise of existing electrochemical remediation platforms toward sensor design and development. In order to enhance the monitoring of these substances, a critical assessment of the requirements for future research, challenges associated with detection, and opportunities is provided. In addition to-or instead of-the now-in-use laboratory-based analytical equipment, these technologies can be utilized to support extensive research and manage issues pertaining to microplastics in the environment and other matrices., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (This journal is © The Royal Society of Chemistry.)

Curcumin (diferuloylmethane), the primary curcuminoid in turmeric rhizome, has been acknowledged as a bioactive compound for numerous pharmacological activities. Nonetheless, the hydrophobic nature, rapid metabolism, and physicochemical and biological instability of this phenolic compound correspond to its poor bioavailability. So, recent scientific advances have found many components and strategies for enhancing the bioavailability of curcumin with the inclusion of biotechnology and nanotechnology to address its existing limitations. Therefore, In this study, copolymerized aqua-gel was synthesized by graft polymerization of poly-acrylic acid (P-AA) on cellulose nanocrystals (CNC), after that Curcuma longa (Cur) was incorporated as dopant (5, 10, 15, and 25 mg) in hydrogel (Cur/C-P) as a stabilizing agent for evaluation of bacterial potential and sewage treatment. The antioxidant tendency of 25 mg Cur/C-P was much higher (72.21 %) than other samples and displayed a catalytic activity of up to 93.89 % in acidic conditions and optimized bactericidal inclinations toward gram-positive bacterial strains. Furthermore, ligand binding was conducted against targeted protein enoyl-[acylcarrier-protein] reductase (FabI) enzyme to comprehend the putative mechanism of microbicidal action of CNC-PAA (CP), Cur/C-P, and curcumin. Our outcomes suggest that 25 mg Cur/C-P hydrogels are plausible sources for hybrid, multifunctional biological activity., Competing Interests: Declaration of competing interest The authors declare no conflict of interest., (Copyright © 2023. Published by Elsevier B.V.)

In this study, BaZr 0.87 Y 0.1 M 0.03 O 3-δ perovskite electrolytes with sintering aids (M = Mn, Co, and Fe) were synthesized by a sustainable approach using spinach powder as a chelating agent and then compared with chemically synthesized BaZr 0.87 Y 0.1 M 0.03 O 3-δ (M = Mn, Co, and Fe) electrolytes for intermediate temperature SOFCs. This is the first example of such a sustainable synthesis of perovskite materials with sintering aids. Structural analysis revealed the presence of a cubic perovskite structure in BaZr 0.87 Y 0.1 M 0.03 O 3-δ (M = Mn, Co, and Fe) samples synthesized by both green and conventional chemical methods. No significant secondary phases were observed in the samples synthesized by a sustainable approach. The observed phenomena of plane shift were because of the disparities between ionic radii of the dopants, impurities, and host materials. The surface morphology analysis revealed a denser microstructure for the electrolytes synthesized via green routes due to metallic impurities in the organic chelating agent. The absence of significant impurities was also observed by compositional analysis, while functional groups were identified through Fourier-transform infrared spectroscopy. Conductivity measurements showed that BaZr 0.87 Y 0.1 M 0.03 O 3-δ (M = Mn, Co, and Fe) electrolytes synthesized by oxalic acid have higher conductivities compared to BaZr 0.87 Y 0.1 M 0.03 O 3-δ (M = Mn, Co, and Fe) electrolytes synthesized by the green approach. The button cells employing BaZr 0.87 Y 0.1 Co 0.03 O 3-δ electrolytes synthesized by the chemical and green routes achieved peak power densities 344 and 271 mW·cm -2 respectively, suggesting that the novel green route can be applied to synthesize SOFC perovskite materials with minimal environmental impact and without significantly compromising cell performance., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Ain, Irshad, Butt, Tabish, Hanif, Khalid, Ghaffar, Rafique, Shawar Kazmi, Siraj, Hafez, Abd-Rabboh, Zmrhalova, Filonova, Medvedev and Motola.)

In this work, we address two concerns at once: waste reduction and the development of a lead removal adsorbent. The potential of Lupinus albus seed hull (LSH) powder as an efficient, innovative, and economical adsorbent for Pb(II) absorption was examined in this study. Fourier transform infrared spectroscopy, energy-dispersive X-ray spectroscopy, and scanning electron microscopy investigations were used to determine the structural and morphological properties of the LSH adsorbent. The adsorption process was studied in batch mode with multiple process variables (adsorbent dosage of 4.0-20 g/L; solution pH of 1.5-5.5; contact time of 15-70 min). By fitting the equilibrium data to the Langmuir isotherm model, the maximum adsorption capacity of Pb(II) was 357.14 mg/g at optimized pH (5.5), LSH dose (0.4 g), and interaction time (60 min) with starting Pb(II) concentration of 50 mg L -1 . As for the reaction kinetics, the pseudo-second-order model was shown to be a convenient match. LSH can be reused after four desorption/adsorption cycles and has a high potential for eliminating Pb(II) from wastewater., Competing Interests: The authors declare no competing financial interest., (© 2023 The Authors. Published by American Chemical Society.)

Manganese dioxide (MnO 2 ) nanorods and (3, 6, and 9 mL) chitosan grafted polyacrylic acid (CS-g-PAA) doped MnO 2 were prepared hydrothermally. The study objective is to decrease the recombination rate of MnO 2 upon doping to enhance the dye degradation efficiency and antimicrobial activity. The doping-dependent properties of CS-g-PAA on phase identification, functional groups, optical characteristics, elemental compositions, and morphological analyses of MnO 2 nanorods were conducted using systematic characterization techniques. XRD pattern shows that MnO 2 has a tetragonal structure, with increased crystallite size (15.87 to 29.36 nm) upon doping. The TEM analysis showed that MnO 2 has nanorods and that CS-g-PAA doped MnO 2 displayed nanoflakes-like structures. The decrease in electron-hole pair recombination rate on doping was verified by PL spectroscopy, demonstrating the enhanced catalytic activity. Moreover, adding grafted binary polymers to MnO 2 inhibits bacterial cell growth by binding with the negatively charged cell wall and preventing biofilm formation. The 9 mL doped sample displayed a maximum degradation (99.27 %) in a neutral medium and 85.84 % antimicrobial efficiency against E. coli. The enoyl-acyl carrier protein reductase (FabI E. coli ) and DNA gyrase (E. coli) were inhibited by these CS-g-PAA doped MnO 2 nanostructures (NSs), as shown by in silico molecular docking studies., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Herein, Bi 2 O 3 quantum dots (QDs) have been synthesized and doped with various concentrations of graphitic carbon nitride (g-C 3 N 4 ) and a fixed amount of carbon spheres (CS) using a co-precipitation technique. XRD analysis confirmed the presence of monoclinic structure along the space group P 2 1 / c and C 2/ c . Various functional groups and characteristic peaks of (Bi-O) were identified using FTIR spectra. QDs morphology of Bi 2 O 3 showed agglomeration with higher amounts of g-C 3 N 4 by TEM analysis. HR-TEM determined the variation in the d -spacing which increased with increasing dopants. These doping agents were employed to reduce the exciting recombination rate of Bi 2 O 3 QDs by providing more active sites which enhance antibacterial activity. Notably, (6 wt%) g-C 3 N 4 /CS-doped Bi 2 O 3 exhibited considerable antimicrobial potential in opposition to E. coli at higher values of concentrations relative to ciprofloxacin. The (3 wt%) g-C 3 N 4 /CS-doped Bi 2 O 3 exhibits the highest catalytic potential (97.67%) against RhB in a neutral medium. The compound g-C 3 N 4 /CS-Bi 2 O 3 has been suggested as a potential inhibitor of β-lactamase E. coli and DNA gyrase E. coli based on the findings of a molecular docking study that was in better agreement with in vitro bactericidal activity., Competing Interests: No conflict of interest., (This journal is © The Royal Society of Chemistry.)

In the present study, a new series of 1,2,3-triazole derivatives was synthesized via a click one-pot reaction. The synthesized compounds were found to be active during molecular docking studies against targeted protein 1T69 by using the Molecular Operating Environment (MOE) software. The designed and synthesized compounds were characterized by using FT-IR, 1 H-NMR and LC-MS spectra. The synthesized triazole moieties were further screened for their α-amylase and α-glucosidase inhibitory activities. The preliminary activity analysis revealed that all the compounds showed good inhibition activity, ranging from moderate to high depending upon their structures and concentrations and compared to the standard drug acarbose. Both in silico and in vitro analysis indicated that the synthesized triazole molecules are potent for DM type-II. Out of all the compounds, compound K-1 showed the maximum antidiabetic activity with 87.01% and 99.17% inhibition at 800 µg/mL in the α-amylase and α-glucosidase inhibition assays, respectively. Therefore these triazoles may be further used as promising molecules for development of antidiabetic compounds.

The 7-quinolinyl-bearing triazole analogs were synthesized (1d-19d) and further assessed in vitro for their inhibitory profile against α-amylase andα-glucosidase. The entire analogs showed a diverse range of activities having IC 50 values between 0.80 ± 0.05 µM to 40.20 ± 0.70 µM (α-amylase) and 1.20 ± 0.10 µM to 43.30 ± 0.80 µM (α-glucosidase) under the positive control of acarbose (IC 50 = 10.30 ± 0.20 µM) (IC 50 = 9.80 ± 0.20 µM ) as the standard drug. Among the synthesized scaffolds, seven scaffolds 12d , 10d , 8d , 9d , 11d , 5d , and 14d showed excellent α-amylase and α-glucosidase inhibitory potentials with IC 50 values of 4.30 ± 0.10, 2.10 ± 0.10, 1.80 ± 0.10, 1.50 ± 0.10, 0.80 ± 0.05, 5.30 ± 0.20, and 6.40 ± 0.30 µM (against α-amylase) and 3.30 ± 0.10, 2.40 ± 0.10, 1.20 ± 0.10, 1.90 ± 0.10, 8.80 ± 0.20, 7.30 ± 0.40, and 5.50 ± 0.10 µM (against α-glucosidase), respectively, while the remaining 12 scaffolds 19d , 8d , 17d , 16d , 15d , 7d , 4d , 3d , 1d , 2d , 13d and 6   d showed less α-amylase and α-glucosidase inhibitory potentials than standard acarbose but still found to be active. Structure-activity connection studies also showed that scaffolds with electron-withdrawing groups like -Cl, -NO 2 , and -F linked to the phenyl ring had higher inhibitory potentials for -amylase and -glucosidase than scaffolds with -OCH 3 , -Br, and -CH 3 moieties. In order to better understand their binding sites, the powerful scaffolds 11d and 9d were also subjected to molecular docking studies. The results showed that these powerful analogs provide a number of important interactions with the active sites of both of these targeted enzymes, including conventional hydrogen bonding, pi-pi stacking, pi-sulfur, pi-anion, pi-pi, pi-sigma, T-shaped, and halogen (fluorine). Furthermore, various techniques (spectroscopic), including 1H, 13 C-NMR, and HREI-MS mass, were used to explore the correct structure of newly afforded hybrid scaffolds based on quinoline-bearing triazole ring., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Khan, Iqbal, Shah, Maalik, Hussain, Khan, Khan, Pashameah, Alzahrani, Farouk, Alahmdi and Abd-Rabboh.)

For potentiometric sensing of barbital (BAR), unique micro-sized imprinted polymer/multiwalled carbon nanotube (MWCNT)-based sensors are introduced. MWCNT is a lipophilic ion-to-electron transducing substance. A synthetic, described, and integrated barbital sodium molecular imprinted polymer (MIP) was used as a recognition receptor for potentiometric transduction in a plasticized polyvinyl chloride membrane. Methacrylic acid and ethylene glycol dimethacrylic acid are used as the functional monomer and crosslinking agent, respectively, in the synthesis of the MIPs. In the operating concentration range of 1.0 × 10 -3 to 2.0 × 10 -7 M, the sensors' Nernstian slope was -56.8 ± 0.9 mV/decade, with a detection limit of 1.0 × 10 -7 M. The sensor displayed an accurate response time of 10 s and consistent potential response in the pH range of 8.5-11. Using chronopotentiometry tests, the interfacial capacitance of the presented ion-to-electron transducer was assessed. When compared to sensors without MWCNTs, the interfacial double-layer capacitance for sensors based on those layers reached 52.5 μF. After the addition of the MWCNTs nanocomposite layer, the water layer was eliminated between the sensing membrane and the conducting substrate. A wide range of applications for the proposed sensors for BAR detection in real samples can be provided by the sensors' strong selectivity over the interfering species. The suggested sensors were successfully used to determine BAR in urine samples that had been spiked., Competing Interests: The authors declare no competing financial interest., (© 2022 The Authors. Published by American Chemical Society.)

The synthesis of MMT and poly( o -anisidine) (MMT/POA) clay nanocomposites was carried out by using the chemical oxidative polymerization of POA and MMT clay with POA, respectively. By maintaining the constant concentration of POA, different percentage loads of MMT clay were used to determine the effect of MMT clay on the properties of POA. The interaction between POA and MMT clay was investigated by FTIR spectroscopy, and, to reveal the complete compactness and homogeneous distribution of MMT clay in POA, were assessed by using scanning-electron-microscope (SEM) analysis. The UV-visible spectrum was studied for the optical and absorbance properties of MMT/POA ceramic nanocomposites. Furthermore, the horizontal burning test (HBT) demonstrated that clay nanofillers inhibit POA combustion.

Well-defined silver nanoparticles were doped into bio-based amorphous silica (Ag-b-SiO 2 ) with different silver contents (from 2 to 20 wt%) by a solvent-free procedure. The four as-synthetized samples were hydrogenated at 300 °C to ensure the formation of zero-valent Ag nanoparticles. The prepared samples were characterized by X-ray powder diffraction (XRD), elemental analysis, N 2 sorption measurements, scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and high-resolution transmission electron microscopy (HR-TEM). The characterization data confirmed the formation of well-defined zero-valent silver nanoparticles in the range of 3-10 nm in the low-loading samples, while in high-loading samples, bulky particles of silver in the range of 200-500 nm were formed. The in vitro cytotoxic activities of the Ag-b-SiO 2 samples were tested against the tumor cell lines of breast (MCF-7), liver (HepG2), and colon (HCT 116) over a concentration range of 0.01 to 1000 g. The prepared samples exhibited a wide range of cytotoxic activities against cancer cells. An inverse relationship was observed between the silver nanoparticles' size and the cytotoxic activity, while a direct relationship between the silver nanoparticles' size and the apoptotic cell death was noticed.

Bio-based silica, lignocellulose, and activated carbon were simply produced via the recycling of Hassawi rice biomass waste of Al-Ahsa governorate in the eastern Saudi Arabia region using a fast chemical treatment procedure. Rice husk and rice straw wastes were collected, ground, and chemically treated with sodium hydroxide to extract silica/silicate from the dried plant tissues. The liquid extract is then treated with acid solutions in order to precipitate silica/silicate at neutral medium. Lowering the pH of the supernatant to 2 resulted in the precipitation of lignocellulose. Thermal treatment of the biomass residue under N 2 gas stream resulted in activated carbon production. Separated products were dried/treated and characterized using several physical examination techniques, such as FT-IR, SEM/EDX, XRD, and Raman spectroscopy in order to study their structure and morphology. Silica and lignocelluloses products were then preliminarily used in the treatment of wastewaters and water-desalination processes.

Caffeine is a psychoactive drug that is administered as a class II psychotropic substance. It is also considered a component of analgesics and cold medicines. Excessive intake of caffeine may lead to severe health damage or drug addiction problems. The assessment of normal caffeine consumption from abusive use is not conclusive, and the cut-off value for biological samples has not been established. Herein, new cost-effective and robust all-solid-state platforms based on potentiometric transduction were fabricated and successfully utilized for caffeine assessment. The platforms were modified with reduced graphene oxide (rGO). Tailored caffeine-imprinted polymeric beads (MIPs) based on methacrylic acid (MAA) and ethylene glycol dimethacrylate (EGDMA) were prepared, characterized, and used as recognition receptors in the presented potentiometric sensing devices. In 50 mM MES buffer, the sensors exhibited a slope response of 51.2 ± 0.9 mV/decade (n = 6, R2 = 0.997) over the linear range of 4.5 × 10−6−1.0 × 10−3 M with a detection limit of 3.0 × 10−6 M. They exhibited fast detection of caffeinium ions with less than 5 s response time (<5 s). The behavior of the presented sensors towards caffeinium ions over many common organic and inorganic cations was evaluated using the modified separate solution method (MSSM). Inter-day and intra-day precision for the presented analytical device was also evaluated. Successful applications of the presented caffeine sensors for caffeine determination in commercial tea and coffee and different pharmaceutical formulations were carried out. The data obtained were compared with those obtained by the standard liquid chromatographic approach. The presented analytical device can be considered an attractive tool for caffeine determination because of its affordability and vast availability, particularly when combined with potentiometric detection.

An acid hydrazide derivative was synthesized and transformed into a variety of valuable N-heterocycles such as pyridazinone, oxadiazole, triazolopyridazinone, and triazole derivatives via reactions with certain carbon electrophiles such as 4-methoxybenzaldehyde, indole-3-carbaldehyde, pentan-2,4-dione, and carbon disulfide. The chemical structures of all prepared compounds were verified via their analytical and spectroscopic data. The insecticidal activity of the N-heterocycles was evaluated against field and lab strains of the third larval instar of Culex pipiens . All tested compounds exhibited higher larvicidal activity against the lab strains compared to the field strains, with dissimilar ratios. The obtained results demonstrate that the high toxicity achieved by oxadiazole followed the order of furanone, pyridazinone and hydrazide, with lower LC 50 values of the hydrazone and N -acetylpyridazinone derivatives compared to that of imidacloprid. Interestingly, these compounds are promising agents for insect pest control, especially since they are insoluble in water and can overcome the disadvantages of neonicotinoid applications in pest management programs., Competing Interests: No potential conflict of interest was reported by the author(s)., (This journal is © The Royal Society of Chemistry.)

Hybrid polymer films of polyvinyl pyrrolidone (PVP)/polyvinyl alcohol (PVA) embedded with gradient levels of Bi-powder were prepared using a conventional solution casting process. XRD, FTIR, and SEM techniques have been used to examine the micro/molecular structure and morphology of the synthesized flexible films. The intensities of the diffraction peaks and transmission spectrum of the PVP/PVA gradually declined with the introduction of Bi-metal. In addition, filler changes the microstructure surface of the pure film. The modification in the microstructure leads to an enhancement in the optical absorption characteristic of the blend films. The indirect allowed transition energy was calculated via Tauc's and ASF (Absorption Spectra Fitting) models. The decrease in the hybrid film's bandgap returns to the localized states in the forbidden region, which led the present films to be suitable for photo-electric, solar cell, etc., applications. The relation between the transition energy and the refractive index was studied. The enhancement in the refractive index with Bi-metal concentrations led to use the as-prepared films in optical sensors. The rise of Bi-metal concentrations leads also to the improvement of the nonlinear susceptibility and refractive parameters. The optical limiting characteristics revealed that the higher concentration dopant films reduce the light transmission intensity which is appropriate for laser attenuation and optical limiting in photonic devices. The results suggest that hybrid films are promising materials in a wide range of opto-electronic applications.

Novel cost-effective screen-printed potentiometric platforms for simple, fast, and accurate assessment of Fluoxetine (FLX) were designed and characterized. The potentiometric platforms integrate both the FLX sensor and the reference Ag/AgCl electrode. The sensors were based on the use of 4'-nitrobenzo-15-crown-5 (ionophore I), dibenzo-18-crown-6 (ionophore II), and 2-hydroxypropyl-β-cyclodextrin (2-HP-β-CD) (ionophore III) as neutral carriers within a plasticized PVC matrix. Multiwalled carbon nanotubes (MWCNTs) were used as a lipophilic ion-to-electron transducing material and sodium tetrakis [3,5-bis(trifluoromethyl)phenyl] borate (NaTFPB) was used as an anionic excluder. The presented platforms revealed near-Nernstian potentiometric response with slopes of 56.2 ± 0.8, 56.3 ± 1.7 and 64.4 ± 0.2 mV/decade and detection limits of 5.2 × 10 -6 , 4.7 × 10 -6 and 2.0 × 10 -7 M in 10 mM Tris buffer solution, pH 7 for sensors based on ionophore I, II, and III, respectively. All measurements were carried out in 10 mM tris buffer solution at pH 7.0. The interfacial capacitance before and after insertion of the MWCNTs layer was evaluated for the presented sensors using the reverse-current chronopotentiometry. The sensors were introduced for successful determination of FLX drug in different pharmaceutical dosage forms. The results were compared with those obtained by the standard HPLC method. Recovery values were calculated after spiking fixed concentrations of FLX in different serum samples. The presented platforms can be potentially manufacturable at large scales and provide a portable, rapid, disposable, and cost-effective analytical tool for measuring FLX.

The incorporation of tungsten trioxide (WO 3 ) by various concentrations of graphitic carbon nitride (g-C 3 N 4 ) was successfully studied. X-ray diffraction (XRD), Scanning Electron Microscope (SEM), and Diffused Reflectance UV-Vis techniques were applied to investigate morphological and microstructure analysis, diffused reflectance optical properties, and photocatalysis measurements of WO 3 /g-C 3 N 4 photocatalyst composite organic compounds. The photocatalytic activity of incorporating WO3 into g-C 3 N 4 composite organic compounds was evaluated by the photodegradation of both Methylene Blue (MB) dye and phenol under visible-light irradiation. Due to the high purity of the studied heterojunction composite series, no observed diffraction peaks appeared when incorporating WO 3 into g-C 3 N 4 composite organic compounds. The particle size of the prepared composite organic compound photocatalysts revealed no evident influence through the increase in WO 3 atoms from the SEM characteristic. The direct and indirect bandgap were recorded for different mole ratios of WO 3 /g-C 3 N 4, and indicated no apparent impact on bandgap energy with increasing WO 3 content in the composite photocatalyst. The composite photocatalysts' properties better understand their photocatalytic activity degradations. The pseudo-first-order reaction constants (K) can be calculated by examining the kinetic photocatalytic activity.

All-solid state screen-printed electrodes were fabricated for chlorogenic acid (CGA) detection. The screen-printed platforms were modified with multi-walled carbon nanotubes (MWCNTs) to work as a lipophilic solid-contact transducer. The sensing-membrane was plasticized with a suitable solvent mediator and incorporating [Ni II (bathophenanthroline) 3 ][CGA] 2 complex as a sensory material. In a 30 mM phosphate solution (buffer, pH 6), the sensor revealed a Nernstian-response towards CGA ions with a slope of -55.1 ± 1.1 ( r 2 = 0.9997) over the linear range 1.0 × 10 -7 to 1.0 × 10 -3 (0.035-354.31 μg mL -1 ) with a detection limit 7.0 × 10 -8 M (24.8 ng mL -1 ). It revealed a stable potentiometric response with excellent reproducibility and enhanced selectivity over several common ions. Short-term potential stability and the interfacial sensor capacitance was estimated using both electrochemical-impedance spectroscopy (EIS) and chronopotentiometry techniques. The presented electrochemical platform revealed the merits of design simplicity, ease of miniaturization, good potential-stability, and cost-effectiveness. It is successfully applied to CGA determination in different coffee beans extracts and juice samples. The data obtained were compared with those obtained by liquid chromatography reference method (HPLC)., Competing Interests: The authors declare that there are no conflicts of interest. All authors have approved the manuscript and agree with the submission to your esteemed journal., (This journal is © The Royal Society of Chemistry.)

Novel paper-based potentiometric platforms for rapid, cost-effective, and simple determination of the salicylhydroxamic acid (SHAM) drug are presented. Both the SHAM sensor and the reference Ag/AgCl electrode were integrated together on the miniaturized paper platforms. The ion-sensing membrane for the presented sensor is based on the use of Sn IV -tetraphenylporphyrin (Sn IV TPP) as a charged carrier within a plasticized poly(vinyl chloride) (PVC) matrix. Multiwalled carbon nanotubes (MWCNTs) were used as an ion-to-electron transducer. The resulting sensor revealed a rapid and stable response with a Nernstian slope of -59.3 ± 0.7 mV/decade over the linear range of 1.0 × 10 -6 to 1.0 × 10 -3 M and a detection limit of 0.7 μM. All measurements were carried out in 30 mM phosphate-buffered solution (PBS) at pH 7.2. Intra- and interday precision were measured and found to be 1.7%. The relative standard deviation (RSD%) ( = 5) was calculated as 2.43% after utilizing five different electrodes ( n = 5). The selectivity behavior of the prepared electrodes in the absence and presence of ionic additives was evaluated. The selectivity pattern showed a non-Hofmeister selectivity pattern in the existence of anionic additives with enhanced potentiometric selectivity for SHAM over different lipophilic anions (e.g., ClO 4 - , SCN - , and I - ). The presented device was successfully applied for SHAM determination in pharmaceutical preparations. This paper-based analytical device can be potentially manufactured at large scales and provides a portable, rapid, disposable, and cost-effective analytical tool for measuring the SHAM drug., Competing Interests: The authors declare no competing financial interest., (© 2021 The Authors. Published by American Chemical Society.)

The destruction of toxic pollutants and production of hydrogen gas on the surface of semiconductors under light irradiation is the main significance of photocatalysis. Heterojunctions with matching in band gap energy are urgently required for enhancing the redox power of the charge carriers. A step S-scheme BiVO 3 /SnO 2 nano-heterojunction was carefully synthesized for a successful photodegradation of amaranth dye and photocatalytic hydrogen evolution. Tetragonal SnO 2 nanoparticles of 80 m 2 g -1 surface area and distinct mesoporous structure were fabricated by a sol-gel route in the presence of Tween-80 as the pore structure directing agent. BiVO 3 nanoparticles were deposited homogeneously on the SnO 2 surface in an ultrasonic bath of power intensity 300 W. The photocatalytic efficiency in the destruction of amaranth dye soar with increasing BiVO 3 contents of up to 10 wt%. The hydrogen evolution rate reached 8.2 mmol g -1 h -1 , which is eight times stronger than that of pristine SnO 2 . The sonicated nanocomposites were investigated by XRD, BET, FESEM, HRTEM, EDS, DRS and PL techniques. The step S-scheme heterojunction with superior oxidative and reductive power is the primary key for the exceptional photocatalytic process. The PL of terephthalic acid and the scavenger trapping experiments reveal the charge migration through the step S-scheme mechanism rather than the type (II) heterojunction mechanism., Competing Interests: The authors declare that there is no conflict of interest in the research work., (This journal is © The Royal Society of Chemistry.)

Herein, we describe for the first time, the design and fabrication of a novel nicotine paper-based sensor, in which a miniaturized paper reference electrode is integrated for potentiometric measurements. The paper-based sensors were designed using printed wax barriers to define the electrochemical cell and the sample zones. The electrodes were based on the use of the ion association complexes of the nicotinium cation (Nic) with either tetraphenylborate (TPB) or 5-nitrobarbiturate (NB) counter anions as sensing materials for nicotine recognition. A poly (3,4 ethylenedioxythiophene)/poly-(styrene sulfonate) (PEDOT/PSS) conducting polymer was used as an ion-to-electron transducer. The performance characteristics of the proposed sensors were evaluated and it revealed a rapid and stable response with a Nernstian slope of 55.2 ± 0.3 and 51.2 ± 0.6 mV/decade over the linear range of 1.0 × 10 -5 to 1.0 × 10 -2 M and detection limits of 6.0 and 8.0 μM for [Nic/TPB] and [Nic/NB], respectively. The sensors revealed a constant response over the pH range 3.5-6.5. The designed sensors provided a portable, inexpensive, and disposable way of measuring trace levels of nicotine coming from different cigarettes and in the collected human sweat of heavy smokers. All results were compared favorably with those obtained by the standard gas chromatographic method., Competing Interests: The authors declare no competing financial interest., (© 2021 The Authors. Published by American Chemical Society.)

In recent times, the application of the use of ion-selective electrodes has expanded in the field of pharmaceutical analyses due to their distinction from other sensors in their high selectivity and low cost of measurement, in addition to their high measurement sensitivity. Cost-effective, reliable, and robust all-solid-state potentiometric selective electrodes were designed, characterized, and successfully used for pholcodine determination. The design of the sensor device was based on the use of a screen-printed electrode modified with multiwalled carbon nanotubes (MWCNTs) as a solid-contact transducer. Tailored pholcodine (PHO) molecularly imprinted polymers (MIPs) were prepared, characterized, and used as sensory receptors in the presented potentiometric sensing devices. The sensors exhibited a sensitivity of 31.6 ± 0.5 mV/decade ( n = 5, R 2 = 0.9980) over the linear range of 5.5 × 10 -6 M with a detection limit of 2.5 × 10 -7 M. Real serum samples in addition to pharmaceutical formulations containing PHO were analyzed, and the results were compared with those obtained by the conventional standard liquid chromatographic approach. The presented analytical device showed an outstanding efficiency for fast, direct, and low-cost assessment of pholcodine levels in different matrices.

A simple and cost-effective approach is proposed for silicate ion determination. The approach is based on designing an all-solid-state potentiometric sensor. The plasticized polyvinyl chloride (PVC) membrane sensor is based on the ion-association complex [Ni(bphen) 3 ] 2+ [SiO 3 ] 2- as a sensory recognition material. The sensor is modified with multi-walled carbon nanotubes (MWCNTs) as an ion-to-electron transducer material. The performance characteristics of the new silicate-selective electrode were evaluated using a potentiometric water-layer test, potentiometric measurements, impedance spectroscopy, and current-reversal chronopotentiometry. The developed electrodes exhibited a low detection limit (0.11 μg mL -1 ) over a wide linear range (4.0 × 10 -6 to 1.0 × 10 -3 M) and near-Nernstian sensitivity (slope = -28.1 ± 1.4 mV per decade). They presented a very short response time (<5 s) over the pH range 6-12 and provided acceptable reliability, ease of design and miniaturization, and high potential stability, in addition to good accuracy and precision. The sensors exhibited enhanced selectivity for silicate over many common interfering anions, such as SO 4 2- , NO 3 - , CH 3 COO - , CO 3 2- , Cl - , S 2- , and PO 4 3- . These results could qualify the developed sensor to be used in a successful way for the trace determination of silicate ions in different matrices. The developed method was successfully applied to the potentiometric detection of silicate in different pre-packaged bottled drinking water samples.

Robust, reliable and cost-effective paper-based analytical device for potentiometric pholcodine (opiate derivative drug) ion sensing has been prepared and characterized. A printed pholcodinium (PHL) 2+ /5-nitrobarbiturate (NB) - ion-association complex as a sensory material-based all-solid-state ion-selective electrode (ISE) on a chemically reduced graphene oxide (CRGO) solid-contact, and a printed all-solid-state Ag/AgCl reference electrode, has been combined on a hydrophobic paper substrate coated with fluorinated alkyl silane (CF 3 (CF 2 ) 7 CH 2 CH 2 SiCl 3 , C F 10 ). The sensors revealed a potentiometric slope of 28.7 ± 0.3 mV dec -1 ( R 2 = 0.9998) over a linear range starting from 2.0 × 10 -7 M to 1.0 × 10 -2 M and a detection limit of 0.04 μg mL -1 . The repeatability and stability of the pholcodine paper-based sensor was found to be 2.32%. The RSD% ( n = 6) was found to be 2.67% when using five different paper-based sensors. The sensor revealed an excellent selectivity towards PHL over dextromethorphan, codeine, ephedrine, carbinoxamine, caffeine, ketamine, and K + , Na + and Ca 2+ ions. It showed a good recovery (94-104%) for the determination of PHL in different artificial serum samples. The presented paper-based analytical device was successfully introduced for PHL determination in different pharmaceutical formulations ( i.e. syrups and suspensions) containing pholcodine. The current work can be considered as a promising possible analytical tool to obtain cost-effective and disposable paper-based potentiometric sensing devices. These devices can be potentially manufacturable at large scales in pharmaceutical, clinical and forensic applications for opiate drug assessment., Competing Interests: The authors declare that there are no conflicts of interest. All authors have approved the manuscript and agree with the submission to your esteemed journal., (This journal is © The Royal Society of Chemistry.)

Background: The importance of recognizing and quantifying chemical anions/cations found in various types of samples, including environmental and biological samples, has been extensively studied. Recent findings suggest the possibility of health risks caused by organic compound dimethylarsinic acid (DMAs) rather than its inorganic arsenic metabolite., Objective: This article aims to fabricate polymeric-membrane electrochemical sensors with high sensitivity and selectivity for the cacodylic acid sodium salt dimethylarsinate (DMAs) based on silver diethyldithiocarbamate (AgDDTC) and CuIIphthalocyanine (CuPC) as novel neutral carriers and their applications., Method: DMAs calibration relations and titrations were carried out using a potentiometric workstation equipped with a double-junction reference electrode, in conjunction with the fabricated working electrodes., Results: Sensors revealed fast and stable anionic response with near-Nernstian slopes (-38.6 ± 0.9 and -31.5 ± 0.6 mV/decade), within concentration ranges (1.7 × 10-5 -1.0 × 10-2 and 3.0 × 10-5 -1.0 × 10-2 M) and detection limits (1.0 × 10-5 and 1.6 × 10-5 M) for AgDDTC- and CuPC-based sensors, respectively. Sensors are characterized by extended life-time, signal stability, high precision and short response times. Selectivity for the cacodylate anion over most common anions was tested for the proposed electrodes. Sensors were satisfactorily applied for DMAs quantification in biological matrices with recoveries ranging between 96.2 and 99.0%. Membrane sensors were interfaced with a flow-through system for continuous monitoring of DMAs. The sensors were tested for the assay of different amino acids based on their reaction with cacodylate, where reaction end points were monitored with the proposed electrodes using direct potentiometric determination and flow injection analysis (FIA)., Conclusions: Potentiometric ion-selective PVC-membrane electrodes based on silver diethyldithiocarbamate (AgDDTC) and CuIIphthalothyanine (CuPC) provide adequate and reliable means for the determination of dimethylarsenate anion (cacodylate anion, DMAs). These membrane electrodes are easy to manufacture, they have the advantages of high selectivity and sensitivity, broad dynamic ranges, low detection limits, quick response times and cost effectiveness. Such properties make these sensors suitable for the assay of DMAs levels in aqueous solutions by direct potentiometry, flow injection and potentiometric titration, as well as in monitoring of the titration end points of the reactions between various amino acids and DMAs anion in aqueous solutions., Highlights: Simple electrochemical membranes for dimethylarsinate (DMAs) were prepared, based on diethyldithiocarbamate (AgDDTC) and CuIIphthalocyanine (CuPC). - DMAs sensors were fabricated in two different modules: batch (for static) and flow-through (for hydrodynamic) approaches. - Levels of DMAs were determined in spiked biological samples. - AgDDTC-based sensors were successfully applied in the determination of several amino acids via potentiometric titration with DMAs., (© AOAC INTERNATIONAL 2020. All rights reserved. For permissions, please email: journals.permissions@oup.com.)

An integrated all-solid-state screen-printed ion-selective potentiometric sensor for rapid assessment of sulfite ion in beverages, based on analytical transduction, is described. The constructed potentiometric cell incorporates a polymeric membrane sulfite ion-selective electrode based on cobalt(ii) phthalocyanine (CoPC) as a recognition material and an Ag/AgCl reference electrode with a polyvinyl butyral reference membrane. Two different solid-contact transducers, namely multi-walled carbon nanotubes (MWCNTs) and polyaniline (PANI) were used for a comparative study. The presented sensors exhibited a rapid Nernst response across the concentration ranges from 2.0 × 10 -6 to 2.3 × 10 -3 M and from 5.0 × 10 -6 to 2.3 × 10 -3 M with detection limits equal to 1.1 × 10 -6 M and 1.5 × 10 -6 M for sensors based on MWCNTs and PANI, respectively. The proposed sensors manifested high selectivity and sensitivity, enhanced stability and low cost that provides a wide number of potential applications for food analysis. Good performance characteristics were obtained for the proposed method after applying the validation requirements. Method precision, accuracy, bias, trueness, repeatability, reproducibility, and uncertainty are examined. These analytical capabilities support the rapid and direct determination of sulfite in different beverage samples. The analytical results were verified and compared with the standard iodometric method., Competing Interests: The authors declare that there are no conflicts of interest. All authors have approved the manuscript and agree with the submission to your esteemed journal., (This journal is © The Royal Society of Chemistry.)

All-solid-state potentiometric sensors have attracted great attention over other types of potentiometric sensors due to their outstanding properties such as enhanced portability, simplicity of handling, affordability and flexibility. Herein, a novel solid-contact ion-selective electrode (SC-ISE) based on poly(3,4-ethylenedioxythiophene) (PEDOT) as the ion-to-electron transducer was designed and characterized for rapid detection of harmine. The harmine-sensing membrane was based on the use of synthesized imprinted bio-mimics as a selective material for this recognition. The imprinted receptors were synthesized using acrylamide (AA) and ethylene glycol dimethacrylate (EGDMA) as functional monomer and cross-linker, respectively. The polymerization process was carried out at 70 °C in the presence of dibenzoyl peroxide (DBO) as an initiator. The sensing membrane in addition to the solid-contact layer was applied to a glassy-carbon disc as an electronic conductor. All performance characteristics of the presented electrode in terms of linearity, detection limit, pH range, response time and selectivity were evaluated. The sensor revealed a wide linearity over the range 2.0 × 10 -7 -1.0 × 10 -2 M, with a detection limit of 0.02 µg/mL and a sensitivity slope of 59.2 ± 0.8 mV/hamine concentration decade. A 40 mM Britton-Robinson (BR) buffer solution at pH of 6 was used for all harmine measurements. The electrode showed good selectivity towards harmine over other common interfering ions, and maintained a stable electrochemical response over two weeks. After applying the validation requirements, the proposed method revealed good performance characteristics. Method precision, accuracy, bias, trueness, repeatability, reproducibility, and uncertainty were also evaluated. These analytical capabilities support the fast and direct assessment of harmine in different urine specimens. The analytical results were compared with the standard liquid chromatographic method. The results obtained demonstrated that PEDOT/PSS was a promising solid-contact ion-to-electron transducer material in the development of harmine-ISE. The electrodes manifested enhanced stability and low cost, which provides a wide number of potential applications for pharmaceutical and forensic analysis.

Novel miniaturized poly(vinyl chloride) matrix membrane sensors based on screen-printed carbon electrodes and responsive to bisphenol S (BPS) were formulated. Polymeric membranes are based on an ion-pair complex of BPS anion with an Aliquat 336S counter cation. A solid conductive contact of multi-walled carbon nanotubes (MWCNTs) was used on screen-printed carbon platforms. After drop-casting and drying of the MWCNTs on a carbonaceous substrate, it was coated with a layer of polymeric poly(vinyl chloride) PVC sensing membrane containing the recognition complex. Prepared electrodes revealed a near-Nernstian response towards BPS with a -28.2 ± 0.8 mV/decade anionic slope, 0.02 μg/mL detection limit and 2.5 × 10 -7 - 1.0 × 10 -3 M concentration range (r 2 = -0.9994). Signals were recorded in a 30 mM HCO 3 - /CO 3 2- buffer, pH 10, with fast response times <10 s. A suggested sensing system was effectively applied in the quantitative determination of diminished BPS levels released from plastic bottle samples, and obtained results were statistically assessed against a chromatographic HPLC independent reference method.

A simple potentiometric sensor is described for accurate, precise, and rapid determination of sulfite additives in beverages. The sensor is based on the use of cobalt phthalocyanine as a recognition material, dispersed in a plasticized poly(vinyl chloride) matrix membrane. o -Nitrophenyl octyl ether ( o -NPOE) as a membrane solvent and tri-dodecylmethyl- ammonium chloride (TDMAC) as ion discriminators are used as membrane additives. Under the optimized conditions, sulfite ion is accurately and precisely measured under batch and flow injection modes of analysis. The sensor exhibits fast and linear response for 1.0 × 10 -2 -1.0 × 10 -6 M (800-0.08 µg/mL) and 1.0 × 10 -1 -5.0 × 10 -5 M (8000-4 µg/mL) sulfite with Nernstian slopes of -27.4 ± 0.3 and -23.7 ± 0.6 mV/concentration decade under static and hydrodynamic modes of operation, respectively. Results in good agreement with the standard iodometric method are obtained.Validation of the assay method is examined in details including precision, accuracy, bias, trueness, repeatability, reproducibility, and uncertainty and good performance characteristics of the method are obtained. The sensor response is stable over the pH range of 5 to 7 without any significant interference from most common anions. The advantages offered by the proposed sensor (i.e., wide range of assay, high accuracy and precision, low detection limit, reasonable selectivity, long term response stability, fast response, and long life span and absence of any sample pretreatment steps) suggest its use in the quality control/quality assurance routine tests in beverages industries, toxicological laboratories and by inspection authorities.

The human body is made up of 60 trillion cells, each cell containing 2 millions of genomic DNA in its nucleus. How is this genomic deoxyribonucleic acid [DNA] organised into nuclei? Around 1880, W. Flemming discovered a nuclear substance that was clearly visible on staining under primitive light microscopes and named it 'chromatin'; this is now thought to be the basic unit of genomic DNA organization. Since long before DNA was known to carry genetic information, chromatin has fascinated biologists. DNA has a negatively charged phosphate backbone that produces electrostatic repulsion between adjacent DNA regions, making it difficult for DNA to fold upon itself. In this article, we will try to shed light on how does chromatin package DNA within nucleus and regulate gene expression?, (Copyright © 2017 Elsevier B.V. All rights reserved.)