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Xian-Li Pan,1 Li Nie,1 Shu-Yi Zhao,1 Xiao-Bao Zhang,2 Shan Zhang,2 Zeng-Feng Su2 1Chaohu Hospital Affiliated with Anhui Medical University, Chaohu City, People’s Republic of China; 2Department of General Medicine, Chaohu Hospital Affiliated with Anhui Medical University, Chaohu City, People’s Republic of ChinaCorrespondence: Zeng-Feng Su, Tel +86 0551-82321572, Email suzengfeng@163.comAim: The objective of the study was to clarify the occurrence of atherosclerosis in patients with insomnia.Methods: A total of 60 patients with insomnia and 58 patients in a control group were selected to measure blood glucose, blood lipids and other biochemical and physiological indicators. Brachial-ankle pulse wave velocity (baPWV) was measured to assess arterial stiffness, and color Doppler ultrasound was used to examine carotid artery intima-media thickness (CIMT) and plaque incidence. These indicators were used to determine the occurrence and degree of carotid atherosclerosis, and to compare the differences between the two groups.Results: While there was no significant difference in biochemical or physiological indicators between the two groups (P > 0.05), the baPWV, CIMT, and incidence of carotid plaque in the insomnia group were higher than the control group. In addition, the baPWV, CIMT, and incidence of carotid plaque in the chronic insomnia group were higher than that in the short-term insomnia group. Multiple linear regression analysis was carried out to analyze the effects of insomnia, systolic blood pressure, blood glucose, triglycerides, cholesterol, BMI, age and low-density lipoprotein on atherosclerosis. The results showed that increased CIMT was significantly correlated with insomnia in atherosclerosis group (R2=0.27 on the left, R2=0.37 on the right, P < 0.001).Conclusion: Insomnia correlated with increased arterial stiffness and carotid atherosclerosis, and as the duration of insomnia prolongs, the correlation between them became more obvious.Keywords: insomnia, brachial-ankle pulse wave velocity, carotid intima-media thickness, carotid plaque

Most people living with HIV need antiretroviral therapy to control their infection and experience viral relapse in case of treatment interruption, because of viral reservoir (proviruses) persistence. Knowing that proviruses are very diverse and most of them are defective in treated individuals, we aimed to characterize the HIV blood reservoirs of posttreatment controllers (PTCs), rare models of drug-free remission, in comparison with spontaneous controllers and treated individuals.

There has been a growing interest in benchmarking sequential recommendation models and reproducing/improving existing models. For example, Rendle et al. improved matrix factorization models by tuning their parameters and hyperparameters. Petrov and Macdonald developed a more efficient and effective implementation of BERT4Rec, which resolved inconsistencies in performance comparison between BERT4Rec and SASRec in previous works. In particular, BERT4Rec and SASRec share a similar network structure, with the main difference lying in their training objective/loss function. Therefore, we analyzed the advantages and disadvantages of commonly used loss functions in sequential recommendation and proposed an improved loss function that leverages their strengths. We conduct extensive experiments on two influential open-source libraries, and the results demonstrate that our improved loss function significantly enhances the performance of GRU4Rec, SASRec, SR-GNN, and S3Rec models, improving their benchmarks significantly. Furthermore, the improved SASRec benchmark outperforms BERT4Rec on the ML-1M and Beauty datasets and achieves similar results to BERT4Rec on the ML-20M and Steam datasets. We also reproduce the results of the BERT4Rec model on the Beauty dataset. Finally, we provide a comprehensive explanation of the effectiveness of our improved loss function through experiments. Our code is publicly available at https://github.com/Li-fAngyU/sequential_rec.

Additional file 5: Table S1. The characteristics of patients with CaOx stones and patients with renal cancers. Table S2. ShRNA sequences designed for silencing OCT4 BMP2 and OLMALINC. Table S3 Primer sequences designed for qRT-PCR. Table S4. Primer sequences designed for 5’ and 3’ RACE of OLMALINC. Table S5. The details of primary antibodies used in immunoblotting (IB). Table S6. Primer sequences designed for CHIP-qPCR. Table S7. Primer sequences designed for bisulfite sequencing PCR (BSP) of BMP2 promoter. Table S8. The predicted sites in BMP2 or SMAD4 promoter which OCT4 binds to. Table S9. The intersection of OCT4 binding lncRNAs identified by RIP-Seq and upregulated lncRNAs identified by RNA profiling. Table S10. The full length of OLMALINC identified by 5’ and 3’ RACE in the current study and the transcript variant deposited in the NCBI database.

PurposeTo investigate the changes in immunity and clinical infection events among patients with chronic insomnia.Materials and methodsForty-two patients with chronic insomnia (age = 64.44 ± 10.53) and 47 normal controls (age = 67.08 ± 7.822) were selected to determine differences in data, such as complete blood counts (CBCs), biochemical indices, lymphocyte subsets, immunoglobulin (Ig), complement C3 and C4 and interleukin-6 (IL-6), as well as to compare the incidence of clinical infection between the two groups.ResultsThere were significant differences in erythrocyte, hemoglobin, hematocrit, albumin, globulin, creatinine, IgG, IgG/IgM ratio, CD4+ T-lymphocytes, CD19-lymphocytes, CD4+/CD8+ ratio, platelet/lymphocyte ratio, CD19/CD3 ratio, and clinical infection events between the chronic insomnia group and the control group (p < 0.05). There was no significant difference in neutrophil, lymphocyte, monocyte, and platelet counts; lymphocyte subsets CD8+ T and CD56+; platelet-to-lymphocyte ratio (PLR); neutrophil-to-lymphocyte ratio (NLR); complement C3; complement C4; IgM; IgA; and IL-6 between the experimental group and their controls (p > 0.05). The systolic and diastolic blood pressures of the chronic insomnia group did not vary widely from those of the controls (p > 0.05).ConclusionPatients with chronic insomnia have immunological abnormalities, characterized by a higher incidence of clinical infection.

The Taiyangshan Wetland, a valuable wetland resource in the arid zone of central Ningxia, is critical for flood storage and drought resistance, climate regulation, and biodiversity protection. Nevertheless, the community structure and diversity of bacterioplankton in the Taiyangshan Wetland remains unclear. High-throughput sequencing was used to analyze the differences in bacterioplankton structure and major determinants in the Taiyangshan Wetland from April to October 2020. The composition and diversity of the bacterioplankton community varied significantly in different sampling periods but showed negligible differences across lake regions. Meanwhile, the relative abundances of bacterioplankton Bacteroidetes, Actinobacteria, Firmicutes, Chloroflexi, Tenericutes, Epsilonbacteraeota, and Patescibacteria were significantly different in different sampling periods, while the relative abundances of Cyanobacteria in different lake regions were quite different. Network analysis revealed that the topological attributes of co-occurrence pattern networks of bacterioplankton were high, and bacterioplankton community compositions were complicated in the month of July. A mantel test revealed that the bacterioplankton community in the entire wetland was affected by water temperature, electrical conductivity, dissolved oxygen, salinity, total nitrogen, ammonia nitrogen, chemical oxygen demand, fluoride, and sulfate. The bacterioplankton community structure was affected by ten environmental parameters (e.g., water temperature, dissolved oxygen, salinity, and permanganate index) in April, while the bacterioplankton community was only related to 1~2 environmental parameters in July and October. The bacterioplankton community structure in Lake Region IV was related to seven environmental parameters, including dissolved oxygen, pH, total nitrogen, and chemical oxygen demand, whereas the bacterioplankton community structures in the other three lake regions were related to two environmental parameters. This study facilitates the understanding of the bacterioplankton community in wetlands in arid areas and provides references to the evaluation of aquatic ecological management of the Taiyangshan Wetland.

Bone marrow mesenchymal stem cells (BMMSCs) were proved to play a vital role in multiple myeloma (MM). Polygonatum sibiricum polysaccharide (PSP) was found to have anti-tumor pharmacological effects, yet its interaction with BMMSCs remained poorly understood. Therefore, we explore the effect of PSP on osteogenic differentiation of BMMSCs. BMMSCs were isolated by density gradient centrifugation. CD90 and CD34 were detected by flow cytometry (FCM). Osteogenic marks were detected by quantitative real-time PCR (qRT-PCR) and Western blotting (WB). The vitality of cells treated with different concentrations of PSP was observed by Cell Counting Kit-8 (CCK-8). ALP staining kit was used to detect the activity of alkaline phosphatase (ALP). Alizarin red staining detected the formation of mineralized nodules. Osteoblast-associated genes were evaluated by qRT-PCR and WB. The phosphoinositide 3-kinase (PI3K), protein kinase B (AKT), and mammalian target of rapamycin (mTOR) signaling pathways were tested by WB. The BMMSCs showed good growth under an inverted microscope. FCM showed that CD34 and CD45 was low-expressed, whereas CD44, CD90 and CD105 was highly expressed. Compared with the Control group, the expressions of Runx2 and ALP in cells were significantly increased. CCK-8 showed that different concentrations of PSP had no significant effect on the viability of BMMSCs. BMMSCs treated with 25 mg/l PSP were stained the most deeply by ALP. Mineralized nodules in PSP groups dramatically increased, and hit a peak under the action of 25 mg/l PSP. PSP up-regulated p-PI3K, p-AKT, and p-mTOR, but had no significant effect on PI3K, AKT, and mTOR. PSP induced osteogenic differentiation of BMMSCs from MM patients.

Using bis(tridentate)polypyridyl ligands, two new 2 D (two-dimensional) coordination polymers (CPs), {Cu2(tpbn)(2,6-NDC)2}n (1) and {[Cu2(tphn)(2,6-NDC)2]·4H2O}n (2), where tpbn = N,N′,N″,N‴-tetrak...

BackgroundThe role of serum testosterone levels in male renal stone formation remains controversial. This study aimed to evaluate the relationship between serum testosterone levels and kidney stone prevalence in males.MethodsWe conducted a cross-sectional study based on the data from the National Health and Nutrition Examination Survey 2011–2016, which included 6,633 male participants, to investigate the association between testosterone levels and the prevalence of kidney stones.ResultsIn this study, using the highest quartile of serum testosterone as a reference, a logistic regression model adjusted for confounders in all participants showed that the first quartile (OR: 1.375, p = 0.016), the second quartile (OR: 1.348, p = 0.021), and the third quartile (OR: 1.472, p = 0.003) of testosterone significantly increased kidney stone risks. In the 41–60 age group, the ORs of kidney stone risk in the first, second, and third of serum testosterone were 1.904 (P = 0.005), 1.599 (P = 0.040), and 1.734 (P = 0.015), respectively. This trend can also be found in the 61–80-year group, except in the first quartile of serum testosterone (OR: 1.169, P = 0.436). Adjusted smoothed curves suggest a non-linear relationship between the 8 quantiles of serum testosterone and the risk of kidney stones in all participants and the 61–80 age group and a significant negative relationship in the 41–60 age group (OR: 0.921, P = 0.0193). But no correlation was seen in the 20–40 group.ConclusionsSerum testosterone levels were significantly inversely associated with the prevalence of kidney stones in men over 40 years of age, but no correlation was seen in the 20–40 group. The role of testosterone in stone formation should be redefined, and its effect should be further verified.

A one-pot synthesis of papaverine hydrochloride with 99.6% purity was performed using xylene as solvent for the entire process. The critical parameters of each step, as well as the impurities generated, were identified. The overall yield was improved to 63%. The proposed synthetic procedure is suitable for industrial production.

Additional file 1. Supplementary Material.

Additional file 1: Figure S1. Particle size of TiO2, ZnO and ZIF-8. Particle size was measured using TEM and plotted thereafter. The peak sizes for TiO2, ZnO, and ZIF-8 were 102.1 nm, 114.8 nm and 82.3 nm, respectively. Figure S2. Enlarged figures for (A) Fig. 1C and (B) Fig. 1D. Figure S3. Characterization of ZIF-8 1:2. (A) PXRD pattern. (B) 1H NMR spectrum. (C) XPS spectrum. (D) N2 adsorption and desorption isotherms. (E) Pore size distribution. Figure S4. Characterization of ZIF-8 1:5. (A) PXRD pattern. (B) 1H NMR spectrum. (C) XPS spectrum. (D) N2 adsorption and desorption isotherms. (E) Pore size distribution. Figure S5. Characterization of ZIF-8 1:8. (A) PXRD pattern. (B) 1H NMR spectrum. (C) XPS spectrum. (D) N2 adsorption and desorption isotherms. (E) Pore size distribution. Figure S6. Characterization of ZIF-8 1:16. (A) PXRD pattern. (B) 1H NMR spectrum. (C) XPS spectrum. (D) N2 adsorption and desorption isotherms. (E) Pore size distribution. Figure S7. Physical and chemical characterizations of ZIF-8 (1:2, 1:5, 1:16). (A) TEM images of ZIF-8 (1:2, 1:5, 1:16). (B) Particle sizes of ZIF-8 (1:2, 1:5, 1:16). Particle size was measured using TEM and plotted thereafter. The sizes for ZIF-8 (1:2, 1:5, 1:16) were 164.8 �� 32.6 nm, 102.5 �� 26.8 nm, and 80.0 �� 37.7 nm, respectively. (C) Diffuse reflection spectra for ZIF-8 (1:2, 1:5, 1:16). UV reflectance, especially for UVB and UVC, was enhanced with decreasing the ratios of Zn2+ to 2-MeIM and reached to the highest value for ZIF-8 1:8. UV reflectance was not further increased ZIF-8 1:16. Figure S8. Characterization of TiO2. (A) PXRD pattern. (B) XPS spectrum. Figure S9. Characterization of ZnO. (A) PXRD pattern. (B) XPS spectrum. Figure S10. Characterization of MOF-5. (A) PXRD pattern. (B) 1H NMR spectrum. (C) XPS spectrum. (D) N2 adsorption and desorption isotherms. (E) Pore size distribution. Figure S11. Characterization of IRMOF-1. (A) PXRD pattern. (B) 1H NMR spectrum. (C) XPS spectrum. (D) N2 adsorption and desorption isotherms. (E) Pore size distribution. Figure S12. Characterization of Zn3L3DMF2. (A) PXRD pattern. (B) 1H NMR spectrum. (C) XPS spectrum. (D) N2 adsorption and desorption isotherms. (E) Pore size distribution. Figure S13. Physical and chemical characterizations of zinc-based MOFs. (A-C) TEM images. (A) MOF-5. (B) IRMOF-1. (C) Zn3L3DMF2. (D-F) Particle size distributions of MOF-5, IRMOF-1 and Zn3L3DMF2. Particle size was measured using TEM and plotted thereafter. The sizes were 310.6 �� 142.4 nm, 47.1 �� 13.6 nm, and 256.0 �� 91.3 nm for MOF-5, IRMOF-1, and Zn3L3DMF2, respectively. (G) The zeta potentials of TiO2, ZnO, ZIF-8, MOF-5, IRMOF-1, and Zn3L3DMF2 were 31.7 �� 0.6 mV, 16.4 �� 0.7 mV, 29.5 �� 0.8 mV, ��� 9.9 �� 1.5 mV, ��� 7.0 �� 0.6 mV, and ��� 5.6 �� 0.6 mV, respectively. (H) The degradation of zinc-based MOFs in artificial sweat (pH 6.5, 32 ��C). ZIF-8 (0.5 mg) exhibited the lowest degradation rate relative to that of MOF-5 (0.5 mg), IRMOF-1 (0.5 mg), and Zn3L3DMF2 (0.5 mg). Figure S14. EPR spectra of POBN-OH spin abduct signal produced by TiO2 suspensions in ethanol. (A) TiO2 at 800 ��g mL���1, (B) TiO2 at 50 ��g mL���1. No obvious EPR signal was detected for TiO2 at 50 ��g mL���1. Figure S15. EPR spectra of POBN-OH spin abduct signal produced by suspensions of TiO2, ZnO, and Zn-based MOFs at 800 ��g mL���1 in ethanol. (A) TiO2, (B) ZnO, (C) ZIF-8, (D) MOF-5, (E) IRMOF-1, (F) Zn3L3DMF2. ZnO produced most free radical of ��OH (1.3 �� 1012 spins/mm3), followed by TiO2 (5.9 �� 1011 spins/mm3), MOF-5 (4.1 �� 1011 spins/mm3), Zn3L3DMF2 (2.1 �� 1011 spins/mm3), IRMOF-1 (6.2 �� 1010 spins/mm3) and ZIF-8 (2.3 �� 1010 spins/mm3), suggesting Zn-based MOFs induced much less EPR signal compared to TiO2 and ZnO after UV exposure. Figure S16. UV absorbance of ZIF-8, TiO2, ZnO and 2-MeIM. ZIF-8 showed a higher UV absorbance compared to ZnO. Also, ZIF-8 revealed a higher UVB absorption relative to TiO2, though UVA absorption of is lower. Figure S17. Cell viability of (A) HaCaTs or (B) HEKas after exposed with UV in various doses. Figure S18. Fluorescence images of DNA tail after (A) HaCaTs or (B) HEKas were exposed to UV in various doses. Figure S19. ROS levels in HaCaTs after UVB exposure. (A) Confocal fluorescent images (Blue, nucleus; Green, ROS positive.). (B) Flow cytometry analyses of free radical levels in HaCaTs with/without protections. HaCaTs with ZIF-8 pretreatment showed no obvious increase of ROS. However, ROS were elevated for the cells without protection or with the protections of TiO2, ZnO, MOF-5, IRMOF-1 or Zn3L3DMF2. Figure S20. ROS levels in HEKas after UVB exposure. (A) Confocal fluorescence images (Blue, nucleus; Green, ROS positive.) and (B) flow cytometry analyses of free radicals in HEKas with/without protections. HaCaTs with ZIF-8 pretreatment showed less ROS production relative to that for the cells without protection or with the protections of TiO2, ZnO, MOF-5, IRMOF-1 or Zn3L3DMF2. Figure S21. ROS levels in HaCats after UVA exposure. (A) Confocal fluorescent images (Blue, nucleus; Green, ROS). (B) Flow cytometry analyses of free radical level in HaCats with/without protections. HaCaTs with ZIF-8 pretreatment showed no obvious ROS production. More ROS production were observed in the cells without protection or with the protections of TiO2, ZnO, MOF-5, IRMOF-1 or Zn3L3DMF2. Figure S22. ROS measurement in HEKas after UVA exposure. (A) Confocal fluorescent images (Blue, nucleus; Green, ROS.). (B) Flow cytometry analyses of free radicals in HEKas with/without protections. HEKas with ZIF-8 protection showed less ROS production relative to that for the groups of No protection, IRMOF-1, TiO2, ZnO, MOF-5, or Zn3L3DMF2. Figure S23. UV dose optimization on mouse skin. Images of mouse dorsal skin three days after UV exposure at different doses. UV dose of 206 J m���2 was selected for further use because erythema was observed at this dose. Figure S24. ZIF-8 dose optimization. Images of mouse skin three days after UV exposure with protections of TiO2, ZnO, and ZIF-8 at different doses. The dose of 15% was selected for further in vivo mouse study, because some sunburn was observed for TiO2 and ZnO mice at this dose, while no obvious erythema was observed for ZIF-8 mice at dose of 15%. Figure S25. Digital graphs of mouse dorsal skin three days after UV exposure with the protections of TiO2, ZnO, or ZIF-8. ZIF-8 group showed less ulceration, edema or erythema compared to no protection or glycerol group. Figure S26. ROS in mouse skin after UV exposure. (A) Quantitative and (B) qualitative analyses of free radical level in the skin with/without protections. Figure S27. Microscope photographs of the mouse skin with (A) Masson���s Trichrome staining or (B) IL-1�� immunohistochemistry. UV disturbed collagen distribution and decreased density for no protection and glycerol groups, while ZIF-8 group showed a normal collagen appearance. ZIF-8 also inhibited the skin expression of IL-1�� after UV exposure. Figure S28. UV dose optimization on pig skin. Digital images of pig dorsal skin 24 h after UV exposure. UV at 544 J m���2 could induce obvious erythema. Figure S29. ZIF-8 dose optimization against UV exposure on pig skin. Digital graphs of pig dorsal skin 24 h after UV exposure with protections of ZIF-8 at different doses. ZIF-8 at 15% obviously inhibited erythema formation. Figure S30. Digital graphs of pig dorsal skin 24 h after UV exposure with the protections of TiO2, ZnO, or ZIF-8. ZIF-8 obviously inhibited erythema formation. Figure S31. Blood biochemical analyses after mice were treated with TiO2, ZnO, or ZIF-8 for six times in 15 days. (A-C) Serum levels of (A) AKP, (B) ALT, and (C) AST for liver function analyses. (D-E) Serum levels of (D) BUN, and (E) CRE for kidney function analyses. Both liver and kidney functions were not affected by TiO2, ZnO, or ZIF-8. Figure S32. H&E images of main tissues after mice were treated with TiO2, ZnO, or ZIF-8 for six times in 15 days. No obvious tissue damage was observed for heart, liver, spleen, lung, and kidney in all these groups. Figure S33. The accumulations of Ti or Zn in blood and tissues after TiO2, ZnO, or ZIF-8 were applied for six times in 15 days. (A, B) Ti levels in (A) heart, liver, spleen, lung, and kidney and (B) blood for the mice with TiO2 treatment. (C, D) Zn levels in (C) heart, liver, spleen, lung, and kidney and (D) blood for the mice with ZnO or ZIF-8 treatments.

Additional file 1. Additional figures and table.

Journal of CO2 utilization : JCOU 67, 102322 (2023). doi:10.1016/j.jcou.2022.102322, Published by Elsevier, Amsterdam [u.a.]

To investigate the incidence of Runt-related transcription factor 1 (RUNX1) gene and its associated gene mutations in patients with acute myeloid leukemia (AML), and analyze its clinical characteristics and prognosis.The genomic DNA-PCR method was used to detect the exon of RUNX1 gene, and the gene mutations were analyzed by genetic sequencing. NPM1, DNMT3A, FLT3-ITD, IDH1/2, K/N-RAS, CEPBA, TET2, and WT1 co-mutations were also detected. Patients were followed up to determine efficacy and prognosis.Among 171 patients, the RUNX1 gene mutation was detected in 17 cases, and the mutation rate was 9.9%. The type of RUNX1 gene mutation was 9 missense mutations, 4 frameshift mutations, and 4 nonsense mutations. The peripheral blood leukocyte count of the patients in mutation group was 3 (1-101) ×10AML patients with RUNX1 gene mutation shows unique clinical and biological characteristics, RUNX1 mutation can be regarded as a molecular marker of poor prognosis in AML patients.伴RUNX1基因突变急性髓系白血病患者临床特征的分析.探讨急性髓系白血病（AML）患者中RUNX1基因及其伴随基因突变的发生率，并分析其临床特征及预后。.采用基因组DNA-PCR方法扩增RUNX1基因外显子，应用基因测序分析其基因突变，同时检测NPM1、DNMT3A、FLT3-ITD、IDH1/2、K/N-RAS、CEPBA、TET2、WT1伴随突变的情况，随访患者并判定疗效和预后。.171例AML患者中，17例检测存在RUNX1基因突变，突变率为9.9%。RUNX1基因突变类型为错义突变9例，框移突变4例，无义突变4例，突变组患者初诊时外周血白细胞数[3（1-101）×10伴RUNX1基因突变的AML患者具有独特的临床及生物学特点，RUNX1基因突变可以作为AML患者预后不良的分子学标志。.

Although several miRNAs have been demonstrated to be involved in the influenza virus replication cycle, the identification of miRNAs and mRNAs that are expressed in A549 cells infected with influenza A viruses (IAVs) from different host species has remained poorly studied. To investigate the molecular mechanisms associated with the differential expression of miRNAs during influenza A virus infection, we performed global miRNA and mRNA expression profiling in A549 cells infected with human-origin seasonal influenza A virus H3N2 (Human_Br07), swine-origin influenza A virus H1N1 (SW_3861) or avian-origin influenza A virus H3N2 (AVI_9990). The miRNA and mRNA expression profiles were obtained by microarray and high-throughput sequencing analyses, respectively. The integrated analysis of differentially expressed miRNAs (DEMs) and differentially expressed genes (DEGs) was performed using bioinformatics tools, and the expression of miRNAs and mRNAs was validated by real-time quantitative polymerase chain reaction (RT-qPCR). We identified 20 miRNAs (6 upregulated and 14 downregulated) and 1286 mRNAs (935 upregulated and 351 downregulated) exhibiting the same differential expression trends in three infected groups of cells compared with an uninfected control. An integrated analysis of these expression profiles identified 79 miRNA-mRNA pairs associated with the influenza A reference pathway, and 107 miRNA-mRNA interactions were correlated with the defense of the virus. Additionally, the obtained results were supported by an RT-qPCR analysis of 8 differentially expressed miRNAs (hsa-miR-210-3p, hsa-miR-296-5p, hsa-miR-371a-5p, hsa-miR-762, hsa-miR-937-5p, hsa-miR-1915-3p, hsa-miR-3665, and hsa-miR-1290) and 13 differentially expressed mRNAs (IFNL1, CXCL10, RSAD2, MX1, OAS2, IFIT2, IFI44 L, MX2, XAF1, NDRG1, FGA, EGLN3, and TFRC). Our findings indicate that dysregulated miRNA expression plays a crucial role in infection caused by IAVs originating from different species and provide a foundation for further investigations of the molecular regulatory mechanisms of miRNAs involved in influenza A virus infection.

Purpose: To systematically assess the effectiveness and safety of retrograde flexible ureteroscopy (FURS) versus percutaneous nephrolithotomy (PCNL) in treating intermediate-size renal stones (2-3cm). Materials and Methods: PubMed, Ovid MEDLINE, Web of Science, Cochrane Central Register of Controlled Trials (CENTRAL) and EMBASE were researched to identify relevant studies up to May 2018. Article selection was performed through the search strategy based on Preferred Reporting Items for Systematic Reviews and Meta-Analyses criteria. The Newcastle-Ottawa Scale was applied to assess the methodological quality of case-control studies. Results: Six retrospective case-controlled trials were included for meta-analysis. The pooled results showed that PCNL was associated with a higher initial stone-free rate (SFR). After more complementary treatments, FURS provided a final SFR (OR: 1.69; 95% CI, 0.93-3.05; P = 0.08) comparable to that achieved by PCNL. PCNL was associated with a higher rate of overall intraoperative complications (OR: 1.48; 95% CI, 1.01-2.17; P = 0.04) and longer hospital stay (MD: 2.21 days; 95% CI, 1.11 to 3.30; P < 0.001). Subgroup analysis by Clavien-graded complication showed PCNL had significantly higher rates of minor complications (OR: 1.58; 95% CI, 1.04-2.41; P = 0.03). No significant difference was noted in major complications (OR: 1.14; 95% CI, 0.53-2.45; P = 0.73) or operative times (MD: −9.71 min; 95% CI, −22.02 to 2.60; P = 0.12). Conclusions: Multisession FURS is an effective and safe alternative to PCNL for the management of intermediate-size renal stones (2-3cm). It is advisable to balance the benefits and risks according to the individual characteristics of patients and to decide with patients by discussing the advantages and disadvantages of each procedure.

Propionate is an effective mould inhibitor widely used as a food preservative. In this study, we used zebrafish to explore the adverse effects of long-term exposure to low concentrations of sodium propionate and the underlying molecular mechanisms (from larvae to adult). When exposed for 3 months, we found that blood glucose, total cholesterol, and triglyceride levels increased, and zebrafish developed a hyperglycaemic state. New tank test results showed depression in zebrafish reduced 5-hydroxytryptamine levels in the brain and damaged the dopamine system. At the same time, the results of the color preference test showed that zebrafish had cognitive impairments. In addition, Hypothalamic-Pituitary-Adrenal axis analysis revealed abnormal gene expression, increased cortisol levels, and reduced glucocorticoid receptor mRNA levels, which were consistent with depressive behavior. We also observed abnormal transcription of inflammatory and apoptotic factors. Overall, we found that chronic exposure to sodium propionate induces depressive symptoms. This may be related to the activation of the HPA axis by the hyperglycaemic state, thereby inducing inflammation and disrupting the dopaminergic system. In summary, this study provides theoretical and technical support for the overlap of the emotional pathogenesis associated with diabetes.

Sodium propionate is widely used as a preservative in food. The widespread use of preservatives is known to cause both environmental and public health problems. This study aimed to investigate the effects of sodium propionate on the developmental behavior and glucose metabolism of zebrafish. Our results showed that sodium propionate had no significant effect on the embryonic morphological development of zebrafish embryos but changed the head eye area. Then we found sodium propionate disturbed the thigmotaxis behavior, impaired neural development. Moreover, changes in clock gene expression disrupted the circadian rhythm of zebrafish. Circadian genes regulated insulin sensitivity and secretion in various tissues. Then our results showed that the disorder of circadian rhythm in zebrafish affected glucose metabolism and insulin resistance, which damaged the development of retina. Therefore, the safety of propionate should be further evaluated.

Background and aims: Renal fibrosis is the common outcome in all progressive forms of chronic kidney disease. Unfortunately, the pathogenesis of the renal fibrosis remains largely unexplored, among which metabolic reprogramming plays an extremely crucial role in the evolution of renal fibrosis. Ceria nanoparticles have strong ROS scavenging and anti-inflammatory activity. The present study aims to determine whether CeNPPEG has therapeutic value for renal fibrosis.Methods: Ceria nanoparticles and Ceria nanoparticles-PEG were synthesized according to previously reported procedures with slight modifications. For the in vivo studies, unilateral ureteral obstructive -induced kidney fibrosis was chosen. Through cellular studies, TGF-β1-induced epithelial-to-mesenchymal transition in HK-2 cells of enhanced glycolysis was initially utilized to report the associations among Ceria nanoparticles-PEG, oxidative phosphorylation, glycolysis and renal fibrosis.Results: In this study, Ceria nanoparticles were developed and the Ceria nanoparticles-PEG treatment significantly ameliorated renal fibrosis in unilateral ureteral obstructive mice as well as protected HK-2 cells from transforming growth factor beta1 -induced epithelial-to-mesenchymal transition process. Furthermore, we found that Ceria nanoparticles-PEG suppressed hexokinase 1and hexokinase 2 to block the dysregulated metabolic status, in which damaged epithelial cells take glycolytic metabolism as priority to oxidative phosphorylation, exerting the protective function in the progress of renal fibrosis.Conclusions: The application of Ceria nanoparticles-PEG in this study is attempted to provide another therapeutic option on renal fibrosis.

4-Substituted catechols with different C[double bond, length as m-dash]C bonds as urushiol analogues were synthesized through the a three-step route including reductive amination reaction of 3,4-dihydroxybenzaldehyde with

BACKGROUND: Only a small fraction of patients with colorectal liver metastases (CLM) is eligible for curative resection, and novel treatment options are highly needed. The high linear-energy transfer of short-ranged alpha particles can induce complex double-stranded DNA breaks, leading to cell death. With no known resistance mechanism, alpha-particle emitting isotopes represent a promising tool in cancer management. In targeted alpha therapy (TAT), an alpha-particle emitting isotope is attached to a tumor-targeting antibody (hereafter TAT antibody), ensuring specific delivery to the tumor. By substituting the alpha-particle emitting isotope (Actinium-225, 225Ac) with a diagnostic isotope (Zirconium-89, 89Zr), the in-vivo distribution of the TAT antibody can be monitored using PET/SPECT/CT. The aim of this work was to set up robust labeling and PET/SPECT protocols to evaluate TAT in CLM using novel experimental models. METHODS: For SPECT-imaging of 225Ac daughter isotopes, the combined gamma emission spectra were gated at 218 keV ± 20% and 440 keV ± 20% for Francium-221 and Bismuth-213, respectively. 89Zr was labeled to a desferrioxamine* (DFO*) conjugated TAT antibody, which recognizes both the murine and human antigen. Indium-111 (111In) labeling was performed through diethylenetriaminepentaacetic acid (DTPA) conjugated to an isotype antibody. Radiochemical purity and protein integrity were assessed by size exclusion HPLC, iTLC and SDS-PAGE. For orthotopic CLM model development, 106 cells of human colorectal cancer cell lines HT-55 or LS1034 were injected in the spleen of female Rj:NMRI-Foxn1nu mice, followed by splenectomy. Tumor growth was monitored by T2-weighted MR-imaging. Non-tumor bearing mice (n = 12, 3 per group) were injected with 4 MBq of 10, 20, 50 or 100 µg 89Zr-TAT antibody to evaluate biodistribution and the mice scanned on day 2, 4 and 6 after iv injection. RESULTS: Francium-221 and Bismuth-213 could be separately visualized after free Actinium-225 administration (i.v.). 89Zr and 111In were routinely labeled to their respective antibody with up to 500 MBq/mg with >95% radiochemical purity. 89Zr-DFO*-TAT antibody showed >95% radiochemical purity and >95% protein integrity after 7 days stability test in serum, shown by iTLC and SDS-PAGE. SPECT/CT of 89Zr-DFO*-TAT antibody in non-tumor bearing mice showed little uptake in healthy tissues apart from the liver due to hepatic antibody clearance. Orthotopic models of CLM show reproducible development of metastases for HT-55 and LS1034 within 3 weeks. CONCLUSION: Robust labeling methods, PET/SPECT imaging protocols and suitable animal models of CLM were established. Results of our dual-isotope SPECT/CT imaging of our 89Zr-DFO*-TAT antibody in comparison with an 111In-labeled isotype antibody, together with efficacy data of the TAT, will be presented at the meeting. Citation Format: Frans V. Suurs, Alexander Kristian, Gebregziabher Petros, Yuan Zeng Feng, Karianne G. Fleten, Roger M. Bjerke, Alan Cuthbertson, Kjersti Flatmark. SPECT-imaging guided development and evaluation of targeted alpha therapy (TAT) for colorectal liver metastasis [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 2467.

To investigate the efficacy and safety of total spine endoscopy in the treatment of lumbar disc herniation combined with posterior apophyseal ring separation.From January 2015 to January 2018, a total of 21 patients with lumbar disc herniation complicated with posterior apophyseal ring separation were treated with total spine endoscopy via interlamina approach. There were 17 males and 4 females. The age ranged from 18 to 48 years old and the median age was 27 years old. All were single segment unilateral disc herniation, interlaminar approach was adopted, and the herniated disc was removed unilaterally at the symptomatic side under the microscope, and all or part of the broken bonewas removed.There were no complications such as incision infection, intervertebral space infection, intestinal injury, dural injury and cerebrospinal fluid leakage. The operation time ranged from 32 to 92 minutes and the median time was 57 minutes. Postoperative imaging examination showed that 2 patients had complete resection of osteotomy of posterior edge of vertebral body, 16 patients had partially resection and 3 patients had no resection. All intervertebral discs were completely removed. All 21 patients were followed up, and the duration ranged from 12 to 36 months, with a median of 15 months. The VAS of lumbago was 7.10±1.20 before surgery, 3.46±0.23 on the 3rd day after surgery, 2.36±0.19 on the 6th month after surgery; and the VAS of leg pain was 8.80±0.55 before surgery, 3.54±0.28 on the 3rd day after surgery, and 2.59±0.26 on the 6th month after surgery. The Oswestry Disability Index score was (69.71±9.37)% before surgery, (32.19±6.95)% on the 6th month after surgery, and (20.95± 6.16)% at the latest follow up. Onthe 1st year after operation, 16 patients got an excellent result, 4 good and 1 fair according to Macnab evaluation system.Total spine endoscopy via interlaminal approach can be used as an option in the treatment of lumbar disc herniation combined with vertebral posterior margin dissociation, which can reduce trauma and injury to the lumbar dorsal muscle and achieve similar decompression effect as open surgery. The long term efficacy needs to be further proved by prospective randomized controlled studies with larger sample size.

To study the correlation of the expression alteration of Tim-3 with the T cell and B cell dysfunction in peripheral blood of multiple myeloma (MM) patients.30 patients diagnosed as MM from October 2016 to October 2018 were selected and enrolled in MM group, and 30 healthy persons whose sex and age was matched with the MM patients were selected and enrolled in healthy control group (HC). The blood samples from MM patients and HC were collected, and the peripheral blood mononuclear cells (PBMNC) were separated by density gradient centrifugation, then the serum was kept for further study. The ratios of CD3The ratio of CD3Expression of Tim-3 on CD4 and CD8 cells elevates in the peripheral blood of MM patients, which also correlates with the function suppression of T and B cells.多发性骨髓瘤患者外周血Tim-3的表达变化及与T、B细胞功能异常的关系.研究多发性骨髓瘤（MM）患者外周血中Tim-3的表达变化及其与T细胞、B细胞功能异常之间的的关系.选取2016年10月-2018年10月诊断为MM的30例患者纳入MM组，并选取年龄、性别与之相匹配的30名健康人员纳入对照组（HC）。收集MM患者和HC的外周血，通过密度梯度离心法分离出外周血单个核细胞（PBMNC），同时保留分离过程中留下的血清。应用流式细胞术（FCM）检测PBMNC中CD3MM患者CD3MM患者外周血中Tim-3在CD4和CD8细胞上的表达增加，并与T细胞和B细胞功能抑制存在相关性.

A novel zwitterion named bethanechol chloride (BTCC) was introduced to simultaneously stabilize a Pb-I framework and passivate defects for efficient inverted perovskite solar cells. The BTCC-assisted device yielded an elevated power conversion efficiency of 20.45% with an open-circuit voltage of 1.14 V.

In the Formosa cold seep of the South China Sea (SCS), large amounts of methane and sulfide hydrogen are released from the subseafloor. In this study, we systematically investigated the microbial communities in the seawater–sediment interface of Formosa cold seep using high-throughput sequencing techniques including amplicon sequencing based on next-generation sequencing and Pacbio amplicon sequencing platforms, and metagenomics. We found that Sulfurovum dominated the microbial communities in the sediment–seawater interface, including the seawater close to the seepage, the surface sediments, and the gills of the dominant animal inhabitant (Shinkaia crosnieri). A nearly complete 16S rRNA gene sequence of the dominant operational taxonomic units (OTUs) was obtained from the Pacbio sequencing platforms and classified as OTU-L1, which belonged to Sulfurovum. This OTU was potentially novel as it shared relatively low similarity percentages (

In the past two decades, Cu-doped inorganic semiconductors with near-infrared (NIR) emitting have garnered stupendous research interest. Nevertheless, the incompatibility between the NIR emitting and high photoluminescence quantum yield (PLQY) of the Cu-doped fluorescent probes restricted the extensive application in biological imaging. Herein, the water soluble Cu

The porous plate is the key part for distributing gas in the fluidized bed, which is subjected pressure, tension and shear from flowing gas and material during work. The porous plate is required to have good mechanical properties. The Monel porous plate was prepared by the powder metallurgy process in this paper. Meanwhile, in order to obtain the status of compressive resistance and shear, the compressive property and the shear property were studied. The results showed that the compressive resistance yield strength was 143MPa and elastic modulus was 18.1GPa at the edge of porous plate. In the central part, the compressive resistance yield strength was 67 MPa, elastic modulus was 8.2GPa and elastic strain range was 3 %.The minimum shear strength was 116 MPa in edge and 87 MPa in the central.

FeCrAl alloy is regarded as the optimization material for coal-gasification purification with high temperature, due to its good mechanical property, oxidation resistance and sulfide resistance. This paper focuses on the effect of preoxidation on sulfidation resistance of porous FeCrAl alloy. The continuous and dense Al2O3 film, which was formed on the surface of porous FeCrAl alloy after preoxidation, can prevent the diffusions of atom S and metal ion and improve the sulfidation resistance.

Long noncoding RNA PVT1 is considered to be an oncogene in multiple cancers. Our previous studies indicated that PVT1 levels were higher in bladder cancer tissue and correlated with clinical progression and poor prognosis in bladder cancer patients. A bioinformatics analysis showed that PVT1 may regulate VEGFC expression through miR-128 as a competing endogenous RNA (ceRNA). In this study, we demonstrated that PVT1 expression levels affect the proliferation and migration ability of bladder cancer cells. Moreover, PVT1 knockdown significantly decreased the proliferation capacity of bladder cancer cells in nude mice. Luciferase assays and RNA-binding protein immunoprecipitation were performed to investigate the potential mechanism of ceRNAs in the regulation of PVT1 and VEGFC. The results showed that the increased number of PVT1 transcripts interacted directly with miR-128 to decrease miR-128 binding to the VEGFC 3'-untranslated region. This effect suppressed VEGFC mRNA degradation by miR-128. In conclusion, these results indicated that PVT1 might play a critical role in bladder cancer tumorigenesis via miR-218 and VEGFC. Therefore, PVT1 could be a new biomarker for bladder cancer diagnosis and therapy.

In this study, Chinese yam starch-water suspension (8%) were subjected to high-pressure homogenization (HPH) at 100 MPa for increasing cycle numbers, and its effect of on the physicochemical properties of the starch was investigated. Results of the polarizing microscope observations showed that the starch granules were disrupted (i.e. greater breakdown value) after HPH treatment, followed by a decrease in cross polarization. After three HPH cycles, the crystallinity of starch decreased, while the crystal type remained unaltered. Meanwhile, the contents of rapidly digestible starch and slowly digestible starch were increased. On the contrary, resistant starch content was decreased. Our results indicate that HPH treatment resulted in reduction of starch crystallinity and increase of starch digestibility.

In order to meet the requirements for the purpose of biological implant materials, analyzes the matching requirements of the compatibility and mechanical properties of titanium foam biological; by powder metallurgy method, using TiH2 powder as raw material, using ammonium bicarbonate as pore forming agent, preparation of titanium foam. The influences of pore forming agent content and particle size, sintering temperature and holding time on the pore structure, pore distribution, pore size and compressive properties of foam titanium were discussed in detail. The results show that with the increase of the sintering temperature and prolonging holding time, titanium foam compressive yield strength and modulus increased; with the increase of the content of pore forming agent, titanium foam compression yield strength and Young's modulus decreased. The preparation of a porosity of 48% ~ 77%, pore size between 300 ~ 500 m, foam pore structure and pore size in micron level through three-dimensional pore, pore size of bio materials meet the requirements. The compressive strength is 98~186MPa, and the young's modulus is 1.6 ~ 6.8 GPa, which matches the strength and the modulus of elasticity of biological implants.

This study aimed to produce spherical TiNi powders suitable for additive manufacturing by plasma rotating electrode process (PREP). Scanning electron microscopy, X-ray diffractometry and differential scanning calorimetry were used to investigate the surface and inner micro-morphology, phase constituent and martensitic transformation temperature of the surface and inner of the atomized TiNi powders with different particle sizes. The results show that the powder surface becomes smoother and the grain becomes finer gradually with decreasing particle size. All the powders exhibit a main B2-TiNi phase, while large powders with the particle size ≥178 μm contain additional minor Ti2Ni and Ni3Ti secondary phases. These secondary phases are a result of the eutectoid decomposition during cooling. Particles with different particle sizes have experienced different cooling rates during atomization. Various cooling rates cause different martensitic transformation temperatures and routes of the TiNi powders; in particular, the transformation temperature decreases with decreasing particle size.

Applying the fault detection and diagnosis (FDD) techniques to chillers is beneficial to reduce building energy consumption and to enhance the energy efficiency of buildings. The purpose of this study is to propose a chiller FDD method with better performance for field implementation. The technological paths are as follows: (i) in order to identify new types of faults and to update the FDD fault libraries, a distance rejection (DR) technique is merged into the Bayesian network (BN) by transforming the chiller FDD problem into a single-class classification problem. Furthermore, the DR can be tuned to obtain an adjustable false alarm rate (FAR); (ii) to increase the diagnostic accuracies of known (or existing) faults and the identification accuracies of new types of faults, multi-source non-sensor information (MI) is merged into the BN, i.e., maintenance records and repair service history, healthy states of related equipment and on-site observed information. A novel chiller FDD method based on BN merged DR and MI (DR-MI-BN) is proposed in this study. The performance of this proposed method is evaluated by using the experimental data from ASHRAE RP-1043. Test results show that the FAR can be tuned for different users’ requirements, and that merging the MI significantly improves the diagnostic accuracies of known faults from 77.2% to 99.8% at most (for refrigerant leakage) and the identification accuracies of new types of faults from 56.6% to 99.6% at most (for NF7).

Purpose Juxtaposed with another zinc finger gene 1 (JAZF1) is involved in gluconeogenesis, insulin sensitivity, cell differentiation, lipid metabolism and inflammation, but its role in carcinoma remains inexplicit. Patients and methods We explored the JAZF1 expression in human papillary thyroid cancer (PTC) tissues, adjacent normal thyroid tissues and nodular goitre tissues, as well as Ki67 expression in PTC tissues, using immunohistochemistry staining. Western blotting and RT-qPCR were performed to explore the JAZF1 expression levels in Nthy-ori 3–1, BCPAP and TPC-1 cells. BCPAP cells overexpressing JAZF1 were constructed using an Adv-JAZF1-GFP recombinant adenovirus vector. Next, the cell proliferation assay, colony formation assay, cell cycle analysis, apoptosis and immunofluorescence were performed. The mRNA expression level of nuclear factor-κB p65 (NF-κB p65) was examined using RT-qPCR. The expression of Bcl-2, Bax, transforming growth factor beta-activated kinase 1 (TAK1), NF-κB p65 and NF-κB p-p65 were examined using Western blotting. Results The expression of JAZF1 in human PTC tissues was downregulated compared with adjacent thyroid tissues or nodular goitre. Additionally, JAZF1 expression was associated with the location and lymph node metastasis of PTC. The expression level of JAZF1 had a negative correlation with Ki67 labelling index (LI). Compared to Nthy-ori 3–1 cells and TPC-1 cells, BCPAP cells expressed the lowest JAZF1. JAZF1 overexpressed significantly inhibited proliferation, caused G0/G1 cell cycle arrest and promoted apoptosis in BCPAP cells. Furthermore, JAZF1 overexpressed in BCPAP cells clearly upregulated the expression level of Bax protein, whereas decreased the expression of Bcl-2, TAK1, NF-κB but did not affect the mRNA or protein expression level of NF-κB p65. Conclusion JAZF1 inhibits proliferation and induces apoptosis in BCPAP cells by suppressing the activation of TAK1/NF-κB signalling pathways, suggesting that JAZF1 may serve as a reliable molecular marker in PTC.

The rapid development of mobile communication technologies prompts the emergence of mobile edge computing (MEC). As the key technology toward 5th generation (5G) wireless networks, it allows mobile users to offload their computational tasks to nearby servers deployed in base stations to alleviate the shortage of mobile resource. Nevertheless, various challenges, especially the edge-user-allocation problem, are yet to be properly addressed. Traditional studies consider this problem as a static global optimization problem where user positions are considered to be time-invariant and user-mobility-related information is not fully exploited. In reality, however, edge users are usually with high mobility and time-varying positions, which usually result in users reallocations among different base stations and impact on user-perceived quality-of-service (QoS). To overcome the above limitations, we consider the edge user allocation problem as an online decision-making and evolvable process and develop a mobility-aware and migration-enabled approach, named MobMig, for allocating users at real-time. Experiments based on real-world MEC dataset clearly demonstrate that our approach achieves higher user coverage rate and lower reallocations than traditional ones.

Swine are reservoirs for anthropogenic/zoonotic influenza viruses, and the prevalence and repeated introduction of the 2009 H1N1 pandemic influenza virus (pdm/09) into pigs raises the possibility of generating novel swine influenza viruses with the potential to infect humans. However, studies aiming to identify miRNAs involved in the transfer of novel swine influenza virus infection to human cells are rare. In this investigation, from the view of small RNA, microarrays and high-throughput sequencing were used to detect differentially expressed miRNAs and mRNAs after human lung epithelial cells were infected with the following three stains of influenza viruses: a novel H3N2 swine influenza virus reassorted with pdm/09 fragments, pdm/09 and classical swine influenza virus. A miRNA-mRNA interaction map was generated to show the correlation between miRNAs related to infection by the viruses with human infective potential/capability. The expression of 4 miRNAs (hsa-miR-96-5p, hsa-miR-140-5p, hsa-miR-30a-3p and hsa-miR-582-5p) and 5 relevant mRNAs (RCC1, ERVFRD-1, RANBP1, SCARB2 and RPS29) was determined. The integration analysis indicated that these candidates have rarely been reported to be associated with influenza virus. Focusing on miRNA expression changes could reveal novel reassortant viruses with human infective potential that may provide insight into future pandemics.

Aim Studies have reported that micro (miR)-486-5p plays a crucial part in the progression of leukemia, however, to the best of our knowledge, few studies have been conducted on its mechanism in leukemia. In this study, the mechanism of miR-486-5p in leukemia cells was pointed out and its possible target genes were analyzed for the purpose of providing new therapeutic strategies for treating leukemia patients. Methods MiRNA expression of Leukemia cells (K562, Kasumi-1, and THP-1) and primary leukocytes was detected by Real-time Quantitative polymerase chain reaction(qPCR). The activity of the cells was assessed using the Cell Counting Kit-8 (CCK-8). Apoptotic cells were analyzed by a flow cytometer (FCM). Caspase-3 activation in leukemia cells was determined by Western blot. Targetscan 7.2 was used to predict the potential targets of miR-486-5p and further confirmed by dual-luciferase reporter assay. Result miR-486-5p was significantly down-regulated in leukemia cells. The over-expression of miR-486-5p notably increased the apoptosis and caspase-3 activity in leukemia cells. There was a predicted interaction site for miR-486-5p in the FOXO1 3′-UTR. Furthermore, this study showed that FOXO1 was significantly up-regulated in leukemia cells, the growth of which was depressed by the up-regulation of miR-486-5p. Conclusion miR-486-5p may inhibit the proliferation of leukemia cells and induce apoptosis through targeting FOXO1.

Server consolidation technique plays an important role in energy management and load-balancing of cloud computing systems. Dynamic virtual machine (VM) consolidation is a promising consolidation approach in this direction, which aims at using least active physical machines (PMs) through appropriately migrating VMs to reduce resource consumption. The resulting optimization problem is well-acknowledged to be NP-hard optimization problems. In this paper, we propose a novel merge-and-split-based coalitional game-theoretic approach for VM consolidation in heterogeneous clouds. The proposed approach first partitions PMs into different groups based on their load levels, then employs a coalitional-game-based VM consolidation algorithm (CGMS) in choosing members from such groups to form effective coalitions, performs VM migrations among the coalition members to maximize the payoff of every coalition, and close PMs with low energy-efficiency. Experimental results based on multiple cases clearly demonstrate that our proposed approach outperforms traditional ones in terms of energy-saving and level of load fairness.

Additional file 1: Table S1. Summary of Treatment-Emergent Adverse Events (regardless of causality) Reported in >5% of Patients in Either Dosing Subgroup.

Elevating the operating temperature of polymer electrolyte fuel cells (PEFCs) can facilitate the reaction kinetics and improve the CO tolerance of catalysts, and simplify the complex water-heat management system of fuel cells. In this study, we fabricated the ionic liquid-based polymer electrolyte that can operate up to 250 °C without external humidification. The membrane utilized polybenzimidazole (PBI) as the polymer matrix, and ionic liquids as the proton conductive fillers. Among all the investigated ionic liquids, diethylmethylammonium trifluoromethanesulfonate ([dema][TfO]) shows favorable performance and stability. The PBI-[dema][TfO] composite membrane exhibits high proton conductivity of 108.9 mS cm−1 at 250 °C. The single cell with PBI-[dema][TfO] composite membrane and Pt/C as the electrode shows output performance of 144 and 62 mW/cm2 at 125 °C and 250 °C, respectively. This work demonstrates that it is very promising to fabricate high temperature PEFCs using the ionic liquid-based polymer electrolyte.

This paper analyzed the failure of gas wells in Northwest Oilfield and provided corresponding methods to solve the issue. In order to thoroughly investigated the failure factors, methods used in the experiment were material performance testing, SEM, EDS and XRD. Besides, a self-designed HTHP autoclave was used to simulate the real corrosion environment and thereby a new method to choose suitable materials was proposed. The experimental results showed that the chemical composition, metallographic structure, hardness, microstructure and non-metallic inclusion of the failed tubing were all qualified. As a matter of fact, the failure was caused by water-accumulation corrosion which occurred at 6447 m at the bottom of the wellbore, and by water-carrying corrosion occurred at 5086.4m. This paper concluded that the P110ss steel should be selected as tubing in the failed well at a depth of 5086.4m and nickel base alloy steel should be selected at 6447m.

// Cui Yu 1 , Chen Hequn 1 , Liu Longfei 1 , Wang Long 1 , Chen Zhi 1 , Zeng Feng 1 , Chen Jinbo 1 , Li Chao 1 , Zu Xiongbing 1 1 Department of Urology, Xiangya Hospital, Central South University, Changsha, Hunan 410000, China Correspondence to: Zu Xiongbing, email: zuxbxy@126.com Keywords: GSTM1, GSTT1, polymorphism, bladder cancer Received: September 06, 2016 Accepted: November 16, 2016 Published: November 29, 2016 ABSTRACT Background: Previous studies have indicated association between GSTM1 and GSTT1 gene polymorphisms and bladder cancer susceptibility, but the results have been inconclusive. Here, we performed a meta-analysis to investigate the association between GSTM1 / GSTT1 deletion polymorphisms and bladder cancer susceptibility. Methods: We searched for all studies investigating the association between GSTM1 or GSTT1 polymorphism and bladder cancer susceptibility in Pubmed, Web of Knowledge, and the Cochrane Central Search Library. A systematic review and meta-analysis were performed. Subgroup analyses were performed on different ethnicity, population-based and smoking status. Results: Our search identified 63 studies. GSTM1 null, GSTT1 null and GSTM1 / GSTT1 double-null genotypes were associated with increased risk of bladder cancer (OR: 1.36 95% CI: 1.25-1.47, P

Stone wool external thermal insulation composite system is used more and more in China now. Former studies in China simply consider that water vapour permeability of render coating should be equal to the water vapour permeability of stone wool. Neither is there any necessary indicator for the liquid water (wind-driven rain) for the system. Therefore, an important issue to be solved currently is how to propose reasonable indicators for water vapour and liquid water transfer performance in stone wool system for different climate zones in China. This research studied on the simulation of hygrothermal distribution of stone wool system. Simulations of stone wool systems were done under different outdoor temperature, humidity, wind driving rain and other conditions to analyze the moisture content in each layer of the system. Meanwhile, simulations of different render coating performances were also done to analyze the moisture content in each layer. Based on these, indicators for water vapour and liquid water were proposed, which provides a foundation for the stone wool external thermal insulation composite system standards in China.

Acute compartment syndrome of the lower extremity is a serious postinjury complication that requires emergency treatment. Early diagnosis is of paramount importance for a good outcome. Four muscle compartments in the calf (anterior, lateral, deep posterior, and superficial posterior) may be individually or collectively affected. Acute segmental single-compartment syndrome is an extremely rare condition characterized by high pressure in a single compartment space with threatening of the segmental tissue viability. In this case report, we describe a young man with Achilles tendon rupture who complained of postoperative pain in the anterior tibial region. Emergent computed tomography angiography and magnetic resonance imaging revealed local muscle edema. Segmental anterior compartment syndrome was diagnosed and fasciotomy was performed.

The purpose of this study was to evaluate and compare the outcome of single- and double-elastic stable intramedullary nailing (ESIN) for the treatment of pediatric both-bone forearm fractures. We retrospectively analyzed 49 children with both-bone forearm fractures treated with ESIN. Twenty-four patients were treated with single-ESIN (S-ESIN) to fixate the radius only, and the other 25 patients were treated with double-ESIN (D-ESIN) to fixate the radius and ulna. The duration of surgery, times of fluoroscopy, cost of hospitalization, period of castoff, union time, radiographic outcomes, clinical results, and postoperative complications were compared. The duration of surgery, times of fluoroscopy, and cost of hospitalization were significantly lower in the S-ESIN group; however, the average period of castoff was longer in the S-ESIN group. The incidence of delayed union of the ulna was significantly higher in the D-ESIN than in the S-ESIN group. Although the mean angulation deformity of the ulna in the S-ESIN group was significantly larger than in the D-ESIN group, both of them were acceptable (