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Seed priming with water (hydropriming or HP) has been shown to be beneficial for seed germination and plant growth. However, there is little information on the effects of seed priming with amino acids and casein hydrolysate (CH) compared with HP, particularly in relation to early post-germinative seedling growth under salinity stress. In this study, Italian ryegrass seeds (Lolium multiflorum L.) were primed with 1 mM of each of the 20 protein amino acids and CH (200 mg l−1) before they were germinated in 0, 60 and 90 mM NaCl in Petri dishes for 4 d in darkness. Germination percentage (GP), radicle length (RL) and peroxidase (POD) activity in the root of 4-d-old Italian ryegrass seedlings were investigated. Generally, when the seeds were germinated in 0, 60 and 90 mM NaCl, there was no significant difference in GP of seeds among various priming treatments, except that a higher GP was observed in seeds of HP treatment compared with the non-primed seeds when incubated in 60 mM NaCl. When incubated in 60 and 90 mM NaCl, seedlings from seeds primed with L-methionine or CH exhibited greater RL (greater protection against salinity stress) and higher root POD activity than those from non-primed and hydro-primed seeds. Under salinity stress, there were higher levels of malondialdehyde (MDA) in the root of 4-d-old Italian ryegrass seedlings, a marker of oxidative stress, but seed priming with CH was effective in reducing the salinity-triggered increase in MDA content. These results suggest that priming with L-methionine or CH would be better than HP for the protection of seedling root growth under salinity stress and might be associated with enhanced antioxidative defence against salinity-induced oxidative stress.

Novel aryl hydrocarbon receptor (AhR) agonists were identified in coastal sediments in the Yellow and Bohai Seas by use of a combination of effect-directed analysis (EDA) and in silico prediction. A total of 125 sediments were screened for AhR-mediated potencies using H4IIE-luc bioassay. Great potencies were observed in organic extracts, mid-polar fraction (F2), and subfractions of F2 (F2.6-F2.9) of sediments collected from Nantong, Qinhuangdao, and Yancheng. Less than 15% AhR potencies could be explained by detected dioxin-like PAHs. Full-scan screening analysis was conducted for the more potent fractions using GC-QTOFMS to investigate the presence of unmonitored AhR agonists. A five-step prioritization strategy was applied; 92 candidate compounds satisfied all criteria. Among these chemicals, thirteen were evaluated for AhR efficacy. Six compounds; benz[b]anthracene, 6-methylchrysene, 2-methylbenz[a]anthracene, 1-methylbenz[a]anthracene, 1,12-dimethylbenzo[c]phenanthrene, and indeno[1,2,3-cd]fluoranthene, exhibited significant AhR-mediated efficacies. 1,12-dimethylbenzo[c]phenanthrene and indeno[1,2,3-cd]fluoranthene were identified as novel AhR agonists. Potency balance analysis showed that the six newly identified AhR agonists explained 0.4-100% of the total AhR-mediated potencies determined. Overall, combining EDA and in silico prediction applied in this study demonstrated the benefits of assessing the potential toxic effects of previously unidentified AhR agonists in sediments from the coasts of China and Korea.

Here, we investigated the characteristics of the environmental multimedia distribution of microcystins (MCs) introduced from freshwater discharge through the estuary dam of the Geum River. In addition, the applicability of a passive sampling device (polar organic chemical integrative sampler, POCIS) for monitoring MCs was evaluated. Surface water, suspended solids (SS), sediments, and oysters were collected from the inner and outer estuary dam. Seven MC variants were analyzed using HPLC-MS/MS. POCIS was deployed at three sites over one week, and MCs were monitored for four weeks from August to September 2019. Before POCIS was deployed in the field, compounds-specific sampling rates of MCs were determined as functions of water temperature (10, 20, and 30 °C), flow rate (0, 0.38, and 0.76 m s−1), and salinity (0, 15, and 30 psu) in the laboratory. The sampling rates of MCs in POCIS increased significantly with increasing water temperature and flow rate, whereas salinity did not significantly affect the sampling rates between freshwater and saltwater. The MCs in the Geum River Estuary mainly existed as particulate forms (mean: 78%), with relatively low proportions of dissolved forms (mean: 22%), indicating that MCs were mainly contained in cyanobacterial cells. There was no significant correlation among the concentrations of MCs in water, SS, sediments, and oysters. Time-weighted average concentrations of MCs from POCIS were not significantly correlated with the concentrations of MCs in water and oysters. The metabolites of MCs, including MC-LR-GSH, MC-LR-Cys, MC-RR-GSH, and MC-RR-Cys, were detected in oysters (no metabolites were detected in POCIS). Overall, POCIS can be useful for monitoring dissolved MCs in the aquatic ecosystem, particularly in calculating time-weighted average concentrations, but it seems to have limitations in evaluating the contamination status of total MCs, mainly in particulate form.

Selective retinal therapy (SRT) employs a micro-second short-pulse lasers to induce localized destruction of the targeted retinal structures with a pulse duration and power aimed at minimal damage to other healthy retinal cells. SRT has demonstrated a great promise in the treatment of retinal diseases, but pulse energy thresholds for effective SRT procedures should be determined precisely and in real time, as the thresholds could vary with disease status and patients. In this study, we present the use of a multi-port fiber-based interferometer (MFI) for highly sensitive real-time SRT monitoring. We exploit distinct phase differences among the fiber ports in the MFI to quantitatively measure localized fluctuations of complex-valued information during the SRT procedure. We evaluate several metrics that can be computed from the full complex-valued information and demonstrate that the complex contour integration is highly sensitive and most correlative to pulse energies, acoustic outputs, and cell deaths. The validity of our method was demonstrated on excised porcine retinas, with a sensitivity and specificity of 0.92 and 0.88, respectively, as compared with the results from a cell viability assay.

This study investigated the current contamination status and potential sources of traditional and emerging polycyclic aromatic hydrocarbons (t-PAHs and e-PAHs) in the sediments across a wide area of the Yangtze River, spanning nine cities. Fifty-seven sediment samples were collected in 2019, from which 15 t-PAHs and 11 e-PAHs were analyzed using GC-MSD. In addition, organic carbon (OC), total nitrogen (TN), and carbon and nitrogen stable isotope ratios (δ

This study demonstrates the effects of technologies applied for the development of gasoline direct injection (GDI) engine for improving the brake thermal efficiency (BTE). The test engine has a relatively high stroke to bore ratio of 1.4 with a displacement of 2156 cm3. All experiments have been conducted for stoichiometric operation at 2000 RPM. First, since compression ratio (CR) is directly related to the thermal efficiency, four CR were explored for operation without exhaust gas recirculation (EGR). Then, for the same four CR, EGR was used to suppress the knock occurrence at high loads, and its effect on initial and main combustion duration was compared. Second, the shape of intake port was revised to increase tumble flow for reducing combustion duration, and extending EGR-stability limit further. Then, as an effective method to ensure stable combustion for EGR-diluted stoichiometric operation, the use of twin spark ignition (SI) system is examined by modifying both valve diameters of intake and exhaust, and its effect is compared against that of single spark ignition. In addition, the layout of twin spark ignition was also examined for the location of front-rear and intake-exhaust. To get the maximum BTE at high load, 12 V electronic super charger (eSC) was applied. Under the condition of using 12 V eSC, the effect of intake cam duration was identified by increasing from 260 deg to 280 deg. Finally, 48 V eSC was applied with the longer intake camshaft duration of 280 deg. As a result, the maximum BTE of 44% can be achieved for stoichiometric operation with EGR.

Trophic magnification factor (TMF) of persistent toxic substances (PTSs: Hg, PCBs, PAHs, and styrene oligomers (SOs)) in a coastal food web (12 fish and four invertebrates) was determined in Ulsan Bay, South Korea. The nitrogen stable isotope ratios (δ15N) of amino acids [δ15NGlu−Phe based on glutamic acid (δ15NGlu) and phenylalanine (δ15NPhe)] were used to estimate the trophic position (TPGlu−Phe) of organisms. The TPGlu−Phe of organisms ranged from 1.64 to 3.69, which was lower than TP estimated by δ15N of bulk particulate organic matter (TPBulk: 2.46–4.21). Mercury and CB 138, 153, 187, and 180 were biomagnified through the whole food web (TMF > 1), while other PTSs, such as PAHs and SOs were not (biodilution of SOs firstly reported). In particular, the trophic transfer of PTSs was pronounced in the resident fish (e.g., rock bream, sea perch, Korean rockfish). Of note, CB 99, 101, 118, and 183 were additionally found to be biomagnifying PTSs in these species. Thus, fish residency appears to represent an important factor in determining the TMF of PTSs in the coastal environment. Overall, δ15NGlu−Phe provided accurate TPs of organisms and could be applied to determine the trophic transfer of PTSs in coastal food webs.

In this study, we identified major aryl hydrocarbon receptor (AhR) agonists in the sediments from Yeongil Bay (n = 6) using effect-directed analysis. Using the H4IIE-luc bioassays, great AhR-mediated potencies were found in aromatic fractions (F2) of sediment organic extracts from silica gel column chromatography and sub-fractions (F2.6–F2.8) from reverse phase-HPLC. Full-scan mass spectrometric analysis using GC-QTOFMS was conducted to identify novel AhR agonists in highly potent fractions, such as F2.6–F2.8 of S1 (Gumu Creek). Selection criteria for AhR-active compounds consisted of three steps, including matching factor of NIST library (≥70), aromatic structures, and the number of aromatic rings (≥4). Fifty-nine compounds were selected as tentative AhR agonist candidates, with the AhR-mediated activity being assessed for six compounds for which standard materials were available commercially. Of these compounds, 20-methylcholanthrene, 7-methylbenz[a]anthracene, 10-methylbenz[a]pyrene, and 7,12-dimethylbenz[a]anthracene exhibited significant AhR-mediated potency. Relative potency values of these compounds were determined relative to benzo[a]pyrene to be 3.2, 1.4, 1.2, and 0.2, respectively. EPA positive matrix factorization modeling indicated that the sedimentary AhR-active aromatic compounds primarily originated from coal combustion and vehicle emissions. Potency balance analysis indicated that four novel AhR agonists explained 0.007% to 1.7% of bioassay-derived AhR-mediated potencies in samples.

While the coastal pollution of persistent toxic substances (PTSs) has been widely documented, information on offshore environments remains limited. Here, we investigated the spatial distribution and sources of PTSs in the offshore sediments (n = 34) of South Korea. Sediment samples collected from the Yellow Sea (n = 18), the South Sea (n = 10), and the East Sea (n = 6), in 2017–18 were analyzed for a total of 71 PTSs. Target compounds include 31 PCBs, 15 PAHs, 9 emerging PAHs (e-PAHs), 10 styrene oligomers (SOs), and 6 alkylphenols (APs). Sedimentary PCBs showed relatively low concentrations with no significant difference across the three seas (0.16–6.9 ng g−1 normalized organic carbon, OC). Low-chlorinated PCBs (tri- and tetra Cl-CBs) were predominant (mean: 77%), primarily indicating atmospheric inputs. PAHs widely accumulated in the three seas with low to moderate level (22–250 ng g−1 OC), and dominated by high molecular weight PAHs (4–6 rings). PMF analysis revealed coast-specific PAHs sources; i.e., originated from mainly coke production (77%) in the Yellow Sea, vehicle emissions (68%) in the South Sea, and fossil fuel combustion (49%) in the East Sea. SOs showed significant contamination than other PTSs, with elevated concentrations in the Yellow Sea (mean: 350 ng g−1 OC). APs showed a similar regional distribution to SOs, but concentrations were much lower (mean: 17 ng g−1 OC). SOs and APs seemed to be introduced from rivers and estuaries on the west coast of Korea, where industrial and municipal activities are concentrated, then might be transported to offshore through tide or currents. Overall, the novel data presented for various PTSs in offshore Korean sediments warrant the necessity of a long-term monitoring effort and urgent management practice to protect marine ecosystem.

This study demonstrates the effects of technologies applied for the development of a gasoline direct injection (GDI) engine for improving the brake thermal efficiency (BTE) over 44%. The GDI engine for the current study is an in-line four-cylinder engine with a displacement of 2156cm3, which has relatively high stroke to bore ratio of 1.4 (110mm stroke and 79mm bore). All experiments have been conducted using a gasoline having RON 92 for stoichiometric operation at 2000RPM. First, since compression ratio is directly related to the thermal efficiency, four compression ratios (14.3, 15.2, 15.8 and 17.2) were explored for operation without exhaust gas recirculation (EGR). Then, for the same four compression ratios, EGR was used to suppress the knock occurrence at high loads with high compression ratio (CR), and its effect on initial and main combustion duration was compared. Second, the shape of intake port was revised to increase tumble flow of in-cylinder charge for reducing combustion duration at low and high load, and extending EGR-stability limit further eventually. Then, as an effective method to ensure stable, complete and fast combustion for EGR-diluted stoichiometric operation, the use of twin spark ignition system is examined by modifying both valve diameter of intake and exhaust, and its effect is compared against that of single spark ignition. In addition, the layout of twin spark ignition was also examined for the location of Front-Rear and Intake-Exhaust. To get the maximum BTE at high load, 12V electronic super charger (eSC) was applied. Under the condition of using 12V eSC, the effect of intake cam duration was identified by increasing from 260deg to 280deg. Finally, 48V eSC was applied with the longer intake camshaft duration of 280deg. As a result, the maximum BTE of 44% can be achieved for stoichiometric operation with EGR.

The purpose of the study is to assess the relationship between the prevalence of metabolic syndrome–related risk factors and work tenure among office workers. A 10-year cohort analysis was conducted using employees’ health examinations given to new employees within 1 or 2 years of joining the firm and every 5 years thereafter. Age-adjusted odds ratios (95% confidence interval [CI], p < .05) were calculated based on a contrast test with general estimating equations. Specifically, the age-adjusted odds ratios and CIs of those with 20 ± 1 years of work, compared with those with 1 to 2 years of work, were as follows: triglycerides (≥150 mg/dl) 3.01 (2.11, 4.28), waist circumference (male ≥90 cm) 2.70 (1.63, 4.45), and fasting glucose (≥100 mg/dl) 2.67 (1.78, 4.01). The findings of the relationship between metabolic syndrome risk and work tenure as well as the baseline health data of new employees provide a foundation for developing a workplace health management system.

Experimental study on knocking characteristics in a direct injection turbo-charged gasoline engine was carried out. The thermodynamic analysis was conducted to investigate effects of the combustion phasing and the burning rate on the knocking behavior. The localization of knock events and the characterization of the early flame kernel propagation were conducted with the fiber optic sensor. The advanced combustion phasing and the slower combustion speed generally increased the knocking probability. However, not only quasi-dimensional thermodynamic combustion characteristics but also the spatial parameter such as the flame propagation direction significantly affected the knocking occurrence. From the fiber optic sensor test results, knocking onset location was found to be closely correlated with the flame propagation direction and mainly observed in the opposite side to the main flame propagation direction. The flame propagation direction leaning to the exhaust side was identified to be favorable for the knocking mitigation because the end gas location on hotter exhaust side could be avoided. Engine tests for various squish designs and tumble port designs were implemented to study the effect of the in-cylinder flow, which significantly affects previously discussed knocking-related parameters. As a result, tumble and squish flow significantly increased combustion speed and advanced combustion phasing. Fuel consumption could be also reduced due to suppressed knocking combustion. In addition, new tumble port design enabled the flame propagation to have favorable leaning direction.

Porcine epidemic diarrhea virus (PEDV) is a highly enteropathogenic coronavirus of swine that causes acute enteritis with high mortality in nursery piglets. To date, the cellular factors involved in PEDV replication have not been well defined. The extracellular signal-regulated kinase (ERK) that serves as a critical component of cellular signal transduction pathways to modulate a variety of cellular functions has been shown to regulate several viral infections. In the present study, we found that PEDV activates ERK1/2 early in infection independently of viral replication. The PEDV-induced ERK1/2 activation resulted in the phosphorylation of its downstream substrate Elk-1 in infected cells. Treatment with ERK inhibitors or ERK1/2 knockdown significantly suppressed viral progeny production. Inhibition of ERK activation also diminished viral protein expression and genomic and subgenomic RNA transcription. These findings indicate that the ERK signaling pathway plays an important role in the PEDV life cycle and beneficially contributes to viral infection., Highlights • PEDV infection activates the ERK signaling pathway in vitro. • PEDV phosphorylates the ERK downstream substrate Elk-1. • Chemical inhibition and ERK1/2 knockdown impairs the replication of PEDV. • ERK activation is not associated with PEDV-induced apoptotic cell death. • The ERK signaling pathway plays a central role in PEDV replication.

Background: The purpose of this study is to assume appropriate outpatient consultation time for each clinical department on the ba-sis of measured outpatient consultation time and satisfaction of outpatient.Methods: We surveyed the feeling and satisfactory outpatient consultation time, satisfaction, revisiting intention and recommenda-tion to others to 1,105 patients of single general hospital in Gyeonggi-do and measured their real outpatient consultation time from October 28 to November 27 in 2013. On the basis of satisfaction, we estimated appropriate outpatient consultation time through area under the receiver operating characteristic curve in logistic regression model.Results: Feeling outpatient consultation time was 5.1 minutess, satisfactory outpatient consultation time which was suggested by patient was 6.3 minutes, and real outpatient consultation time was 4.2 minutes. Department which had longest real outpatient con-sultation time was infection (7 minutes) and department which had longest satisfactory outpatient consultation time was neurology (9.4 minutes). From the univariate and the multiple linear regression analysis, real outpatient consultation time was longer in pulmon-ology patient, new patient and afternoon patient, satisfactory outpatient consultation time was longer in infection, neurology, neuro -psychiatry, neurosurgery, and rehabilitation patient. Appropriate real outpatient consultation time was suggested as 5.6 minutes which differentiated high and low satisfied patient group. However, we could not assume appropriate outpatient consultation time for each clinical department because the number of patient who had bad satisfaction was too low.Conclusion: To improve patient’s satisfaction, we hope outpatient reservation system is operated as each patient’s outpatient consul-tation time is at least 5.6 minutes.Keywords: Outpatient; Consultation time; Departments; Appropriate

The present study sought to investigate whether porcine epidemic diarrhea virus (PEDV) induces apoptosis and to elucidate the mechanisms associated with apoptotic cell death after PEDV infection. PEDV-infected cells showed evidence of apoptosis in vitro and in vivo. However, experimental data indicated that the caspase cascade is not involved in PEDV-induced apoptotic cell death. Interestingly, mitochondrial apoptosis-inducing factor (AIF) was found to translocate to the nucleus during PEDV infection, and AIF relocalization was completely abrogated by the presence of cyclosporin A (CsA), an inhibitor of cyclophilin D (CypD) that is an essential component of the mitochondrial permeabilization transition pore (mPTP) complex. CsA treatment resulted in significant inhibition of PEDV-triggered apoptosis and suppressed PEDV replication. Furthermore, direct inhibition of AIF strongly impaired PEDV infection and virus-induced apoptosis. Altogether, our results indicate that a caspase-independent mitochondrial AIF-mediated pathway plays a central role in PEDV-induced apoptosis to facilitate viral replication and pathogenesis., Highlights • PEDV induced apoptotic cell death in vitro and in vivo. • Inhibition of caspase activation did not affect apoptosis or PEDV infection. • Mitochondrial AIF translocated to the nucleus during the course of PEDV infection. • Treatment of CypD or AIF inhibitor strongly impaired apoptosis and PEDV replication. • PEDV-induced apoptosis is mitochondrial AIF dependent and caspase dispensable.

In this paper, the main cause on excitation of the combustion instability which may occur in the hybrid rocket motor with a diaphragm was studied. Hybrid rocket motor propulsion tests considering various experimental conditions such as with a diaphragm or not, a diameter of diaphragm, oxidizer mass flow rate, fuel length, etc were performed, and the combustion visualization for the inside of a hybrid rocket motor with a diaphragm was performed. With these experimental results, it was confirmed that the main cause of a large excitation was the hole-tone, and it was shown that the hole-tone model can be predicted experimental primary pressure oscillation frequency quite well.

Highlights • PEDV infection activated p38 MAPK and JNK1/2 in vitro. • UV-inactivated virus failed to induce p38 MAPK and JNK1/2 activation. • Pharmacological inhibition of p38 MAPK or JNK activation impaired PEDV replication. • SAPK cascades do not affect the apoptosis pathway during PEDV infection. • PEDV exploits the p38 MAPK and JNK signaling pathways for optimal replication., The mitogen-activated protein kinase (MAPK) pathways, which are central building blocks in the intracellular signaling network, are often manipulated by viruses of diverse families to favor their replication. Among the MAPK family, the extracellular signal-regulated kinase (ERK) pathway is known to be modulated during the infection with porcine epidemic diarrhea virus (PEDV); however, involvement of stress-activated protein kinases (SAPKs) comprising p38 MAPK and c-Jun NH2-terminal kinase (JNK) remains to be determined. Therefore, in the present study, we investigated whether activation of p38 MAPK and JNK cascades is required for PEDV replication. Our results showed that PEDV activates p38 MAPK and JNK1/2 up to 24 h post-infection, whereas, thereafter their phosphorylation levels recede to baseline levels or even fall below them. Notably, UV-irradiated inactivated PEDV, which can enter cells but cannot replicate inside them, failed to induce phosphorylation of p38 MAPK and JNK1/2 suggesting that viral biosynthesis is essential for activation of these kinases. Treatment of cells with selective p38 or JNK inhibitors markedly impaired PEDV replication in a dose-dependent manner and these antiviral effects were found to be maximal during the early times of the infection. Furthermore, direct pharmacological inhibition of p38 MAPK or JNK1/2 activation resulted in a significant reduction of viral RNA synthesis, viral protein expression, and progeny release. However, independent treatments with either SAPK inhibitor did not inhibit PEDV-induced apoptotic cell death mediated by activation of mitochondrial apoptosis-inducing factor (AIF) suggesting that SAPKs are irrelevant to the apoptosis pathway during PEDV infection. In summary, our data demonstrated critical roles of the p38 and JNK1/2 signaling pathways in facilitating successful viral infection during the post-entry steps of the PEDV life cycle.

The purpose of this study was to provide informations on what middle school students know and think about food additives, and their purchase behavior of processed foods containing food additives. The data were gathered by questionaries from 360 students attending in the 3 randomly selected middle schools around Gyeonggi Province. The valid 340 out of 360 questionaries were analysed by SPSS/WIN 12.0. As a result, the middle school students showed low interest in 4 kinds of representative food additives such as synthetic seasoning, artificial sweetener, artificial dye, and synthetic preservative. They thought that the food additives is necessary but not improve the quality of food, and may have harmful effects on health. The students who has mother of university graduate, and taught by text book A showed the highest interest. Most students rarely checked the indication of food additives on the processed food package, and they hardly put any effort to avoid the processed foods containing food additives. According to the results of this study, the students were not aware and did not know about the food additives enough, and needs to be educated more and in detail in home economics class.

The structural evolution of 1-mercapto-2-propanol layer on Au(1 1 1) during a treatment in a N 2 flow was investigated using STM and cyclic voltammetry. The pristine layer showed a striped structure of (2√3×√3) with coverage of 0.33. As the SAM was treated in the N 2 flow, the superlattice evolved sequentially and irreversibly to the structures of (√21×√3) and (√3×√3)R30° without any coverage change and ultimately to amorphous domains with coverage less than 0.33. In cyclic voltammetric studies, it was found that the coverage of 1-mercapto-2-propanol was 0.33 and that the N 2 treatment induced a partial desorption of the adsorbed thiol molecules. In addition to this, the reductive stripping potential shifted to the anodic direction, signaling that the SAM of 1-mercapto-2-propanol became unstable. The structural evolution and destabilization of the 1-mercapto-2-propanol layer on Au(1 1 1) was discussed in terms of the attractive force between the OH groups via hydrogen bonding and the repulsive force between the methyl groups.

In the present article, the rheological behavior and thermal conductivity for dispersions of paraffin rods in silicone oil were investigated experimentally by examining the effects of surfactant concentration, particle volume concentration and temperature. The test suspensions at 15°C showed a strong shear-thinning viscosity as the particle concentration increased, whereas the test emulsions at 58°C showed typical Newtonian viscosity behavior. Relative viscosities of the present results agree with the theoretical results for rodlike particle suspensions. Thermal conductivities of the paraffin, silicon oil and mixture were measured with varying temperatures, respectively. Thermal conductivities of paraffin dispersions correlated satisfactorily with the Brailsford-Major equation.

Artificial superlattice of the ferroelectric BaTiO 3 /SrTiO 3 multilayer thin film has been fabricated by pulsed laser deposition. In this hetero epitaxial BTO/STO layers, each layers have been continuously grown on (La 0.5 ,Sr 0.5 )TiO 3 /MgO(100) with a periodicity ranging from (BTO 1 unit cell /STO 1 unit cell ) 125 to (BTO 125 unit cell /STO 125 unit cell ) 1 . The total thickness of BaTiO 3 /SrTiO 3 superlattice was 100 nm. X-ray diffraction results clearly showed that epitaxial BTO/STO superlattice was formed with a various periodicity. The depth profile in the superlattice showed that there was no serious interdiffusion between BaTiO 3 and SrTiO 3 layer by Auger electron spectroscope(AES).

Objectives: Recommended Menu (RM) prepared according to the Target Pattern expected to meet the dietary reference intake (DRI) of nutrients. Nutritional adequacy of RM in 'DRI for Koreans 2015' were analyzed to verify whether such expectation was fulfilled. Methods: Dishes in RM are categorized by 5...

BaTiO3(BTO)/SrTiO3(STO) artificial superlattices have been made on MgO (100) substrates. The periodicity of the BTO/STO layers in the superlattice was varied from one-unit cell to 125-unit cell thickness. The dielectric constant and its nonlinearity (or voltage tunability) showed similar behavior as the periodicity was varied. The voltage tunability of the superlattice increased with decreasing stacking periodicity of the BTO/STO within the critical thickness. Similarly, the lattice distortion, i.e., the ratio of the lattice parameter along surface normal to parallel, of the BTO and STO layers increased with decreasing the periodicity. Remarkable enhancement of the voltage tunability has been achieved. The superlattice exhibited large voltage tunability (94%, the highest value to date) at the periodicity of BTO2-unit cell/STO2-unit cell at which the maximum lattice distortion of each layer was obtained. This suggests that the nonlinear dielectric property of the superlattice is closely related with the la...

The spatial distribution of UV-absorbing compounds (mycosporine-like amino acids, MAAs), was investigated by comparing the phytoplankton community structures in the inner and outer waters of the Kongsfjorden inlet, which is located in arctic Svalbard. Thalassiosira sp. and Phaeocystis sp. were dominant in the outer waters of the Kongfjorden inlet, demonstrating high chlorophyll a (chl a) concentrations and low MAA concentrations in the outer bay waters. However, Kongsfjorden Bay was dominated by Phaeocystis sp. and demonstrated high MAA concentrations despite low chl a concentrations. The carbon fixation rate at a station located inside Kongsfjorden Bay (T05) was significantly photo-inhibited by UV radiation, demonstrating higher production rates of MAA and chl a than at a station (B09) in outer bloom waters. Additionally, the turnover rates of individual MAAs were faster inside the Kongsfjorden Bay than in the outside waters. As a result, the natural phytoplankton community demonstrated different UV adaptation mechanisms according to the phytoplankton species, in this case, Thalassiosira sp. vs. Phaeocystis sp. It is possible to understand real-time changes for newly photosynthesized MAAs as UV-absorbing compounds in the natural phytoplankton community. This takes place via determination of in situ MAA production rates using 13C tracer and High Performance Liquid Chromatography (HPLC) combined with an isotope ratio mass spectrometer (irMS).

The relationship of dietary carotenoid consumption and the concentration of retinol and several carotenoids in mature human milk was investigated. Forty-two lactating mothers of infants from 0.5 to 7 months participated in this study. All measurements of milk retinol and carotenoids were determined by HPLC

Objectives: Maintaining a balanced diet and thus health is crucial for adolescents, and the first step for balanced diet practice is meal planning. Adolescents, however, find it difficult to plan their meals. This study thus was set out to design an easier way of planning meals for adolescent girls. Methods: A dish-based target pattern for adolescent girls was tabulated, and validity of this was examined. Meal plan applying a dish-based target pattern was prepared by 150 female middle school students, and nutritional adequacies of those meal plans were examined. Validity and adequacy were tested by energy content, energy contribution ratio, nutrient adequacy ratio (NAR), probability of nutrient inadequacy, index of nutritional quality (INQ) calculation. Results: A dish-based target pattern with 11 dish groups was validated for nutritional adequacy. Though the NAR of calcium was 0.96, the INQ of calcium was 1.00. The average energy supply from the meal plans was 2,379 kcal, higher than the estimated energy requirement of a female middle school student, but the energy contribution ratio of carbohydrates, proteins, and fats were all adequate according to the acceptable macronutrient distribution range (AMDR). NAR of all nutrients examined were 1.0, except for calcium. The NAR and INQ of calcium were 0.87 and 0.75, respectively, and the meal plans at risk for calcium inadequacy was 19.30%. Conclusions: A dish-based target pattern proposed for adolescent girls was valid, but the meal plan prepared by female middle school students using this approach was high in energy and low in calcium supply. To cut down the energy supply from the meal plan, it is necessary to recommend dishes low in fat and use low fat cooking methods. To increase the calcium supply, it is important to recommend seaweed and legume group dishes with higher Ca INQ food items. Korean J Community Nutr 20(2): 87~95, 2015

Objectives: Maintaining a balanced diet is very crucial for adolescents. However, adolescents, who may have a short notion about the amount of food, find it difficult to plan daily meals by applying the target pattern proposed by the Korean Nutrition Society. This study was carried out to revise the target pattern based on cooked dishes instead of raw material food groups as an easier way for Korean adolescents to plan their meals.

Objectives: Vegetables are the most left over side dishes in school lunch programs. This study intended to analyze the differences in preference for vegetables among the students of different age groups in order to determine potential ways of increasing vegetable consumption in this study group.

Objectives: The purpose of this study was to provide the preliminary data for dietary education to increase students' vegetables intake. Methods: The attitude of vegetables consumption (5-Likert scale), preference score (7Likert scale) and eating frequency (5-Likert scale) of 9 fragrance vegetables were investigated by survey questionnaire. A total of 370 students enrolled in primary, middle, high schools, and university participated in the study and data were analyzed by the SPSS WIN (ver 12.0). Results: About 40% of those surveyed answered that they do not eat some kinds of foods and 16% of students do not eat vegetables, the most unfavorable foods. The students in all groups (primary 2 and 5 , middle and high school, university students) answered that they liked vegetables with the highest score in university students, and they did not often eat fragrance vegetables. Lower age student group, especially primary school 2 showed more positive attitudes of eating challenge toward no experience, bad taste, and dislike but nutritious vegetable foods. The most important factor of vegetable preference was taste, the biggest reason of both like and dislike. Only 4 students designated nutrition as for vegetable dislike reason, means that all students knew about the nutritional importance of vegetables. It was shown that the color and flavor of the vegetables act as dislikable reason rather than likable reason. The significant correlations between preference score and intake frequency of fragrance vegetables were confirmed, and the younger the students the greater the correlation coefficient. Conclusions: Thus providing more chance to experience vegetables, such as fragrance vegetables and education about the importance of balanced diet will be an effective way of increasing vegetables intake, and the younger the students the greater the education effect. Korean J Community Nutr 19(3): 223~230, 2014

Objectives: The objective of this study was to revise the target pattern in food guidance system for adolescents’ balanced menu planning. Methods: The food groups in the target pattern were divided into detailed food items, and intake number were assigned to each food items based on the revised standard food composition table. The validity of revised target pattern was examined. Menu planning according to the revised target pattern was made available to 305 male and female middle school students and the nutritional assessment of the menu plan were carried out using SPSS WIN 12.0. Results: The energy contents, energy contribution ratios of carbohydrate, fat, and protein, and 4 minerals’ and 6 vitamins’ contents of the revised target pattern were adequate. The average energy contents of the menu planned according to revised target pattern were 400~500 kcal higher than that of the revised target pattern when the revised standard food composition was applied. The energy contribution ratios of fat were 28.9%, close to maximum of acceptable macronutrient distribution range (AMDR) (30%), and that of carbohydrate were 54.5%, lower than minimum of AMDR (55%). The nutrient adequacy ratios (NARs) of calcium and vitamin C were less than 1.0. According to index of nutritional quality (INQ) of food items, kimchi, milk⋅dairy products, and soybean curd were energy efficient source for calcium, kimchi, fruit, vegetable and seaweed were energy efficient source for vitamin C, with INQ of food items were higher or close to 2.0. Kimchi was the best energy efficient source of calcium and vitamin C. Conclusions: Revised target pattern based on the adolescent’s foods intake was not good enough for balanced menu planning by adolescents, because what they ate and what they wanted to eat were very much different. Detailed guidance for food selection is necessary in each food items. Korean J Community Nutr 19(3): 274~282, 2014

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It is important to prepare and execute the menu plan for proper and balanced intake of nutrients in the adolescence. This study investigated the new approach for planning menu by ordinary meal pattern based on cooked foods groups. The amounts of cooked foods in the menu plan assumed to be single serving size. The middle school second graders participated for the study. A total of 313 questionnaires were analyzed using CAN-pro 3.0 and SPSS WIN 12.0 program. The average content of energy in the menu plan was 2,453 kcal, the average ratios of energy contribution by carbohydrate, protein and fat were 54.3%, 17.9%, 27.8%, respectively. A total of 56.9% menu plans (94.9% of male and 8.7% of female students’) were below the lowest limit of optimum carbohydrate energy ratio of 55%. A total of 29.1% menu plans (33.1% of male and 23.9% of female students’) were exceed the highest limit of optimum fat energy ratio of 30%. The NAR of minerals and vitamins were all 1.0 except for calcium (0.92) and folate (0.88). When INQ of the individual cooked food groups were calculated, kimch was the highest in all minerals and vitamins examined, suggesting that kimch may be the best source for all minerals and vitamins, including calcium and folate with the minimum change in energy content. In conclusion, the menu plan by ordinary meal pattern in this study was low in carbohydrate, high in fat, and contained enough minerals and vitamins except for calcium and folate for middle school students. (Korean J Community Nutr 18(4) : 333~343, 2013)

The purpose of this study was to provide information on obesity assessment for Koreans. Among total of 1012 research papers enlisted in the Korean J Community Nutrition form 1996 to 2011, 248 articles were examined in which subjects were divided into more than 2 groups by obesity rate. About the method of anthropometric data collection, more than half of the research papers examined 52.5% and 28.7% of studies utilized the directly measured data and self-described data, respectively. About the utilization of obesity assessment methods, indirect methods of weight-height index (BMI, BMI percentile, and Rohrer index) and PIBW (WLR, Broca index, and KDA) were 62.4% and 23.2%, respectably, and the direct method of percent body fat assessment was only 9.3%. The most frequently utilized methods were WLR in under primary and primary school children, and BMI in the middle and high school students and in adults. For primary school students, WLR was the most frequently utilized method up to 2007, but it changed to BMI percentile afterward. Broca Index was no longer utilized since 2008. There were no articles utilizing BMI percentile and Rohrer index for obesity assessment in adults. Criteria for obesity assessment were not consistent among research papers: for example, % body fat, 19~40%; BMI, 20~30; BMI percentile, 85th or 95th. In the case of PIBW, 120% of ideal weight was the most frequently utilized criterion for obesity. Based on these findings, we suggest that proper methods and criteria of obesity assessment for each age group should be determined and proclaimed. (Korean J Community Nutr 18(5) : 525~538, 2013)

The purpose of this study is finding the easy way of 4 categories activity level confirmation for estimated energy requirement calculation. Total of 386, 5th and 6th grade primary school students participated. The time spent on 7 kinds of activity were collected for 1 day by the internet program developed. Judged by the activity coefficient, sedentary were 6.7% and 5.1%, low active 33.2% and 40.4%, active 43.8%, and45.5%, and very active 16.3% and 9.0% for boy and girl, respectively. The highest and significant correlation coefficient between activity coefficient and time spent on activities shown were 0.339 in commute activity for boys, and 0.466 in leisure for girls. The sensitivity of the sedentary conformation by commute hour for boys was 0.79, and that of very active was 0.56. The sensitivity of the sedentary conformation by leisure hour for girls was 0.67, and that of very active was 0.63. The sensitivity of low active and active by 7 different types of activity was quite low, 0.04~0.37. The exact agreement of activity level conformed by easy way developed was 30.8% and 33.7%, for boys and girls, respectively. More accurate way to identify 4 categories activity level needs to be developed, especially sensitive to conformation of low active and active levels. (Korean J Community Nutr 16(3) : 307~314, 2011)

This study was conducted to investigate the time spent, activity coefficient and energy expenditure on 7 different types of activity. Total of 386 5th and 6th grade primary school boys and girls in Seoul participated in this study. The participants spent 8 hrs for rest, 4 hrs for out of school study, 3 hrs for leisure, 1 hr 45 mins. for hygiene, 1 hr for commute, and 15 mins. for house choir activity in the day of 6 class hours. The average activity coefficient of 1 day for boys and girls were 1.69 and 1.64, respectively. The TEEs were 2,382 kcal and 2,050 kcal for boys and girls, respectively. The energy cost for study related activity (in school and study activities) was 40% of the TEE, and for rest 20%, for commute 9%, and for hygiene 10% of the TEE. The energy cost for house choir was only about 2% of TEE. The 91.2% participant's EER was higher than the EER shown in the table of 2010 DRI for Koreans. When the participant's EER was compared with the energy allowance calculated by the method in 2000 RDA for Korean, 81.6% was in the range of ± 5%, and the correlation coefficients between the 2 values were 0.981 for boys and 0.978 for girls, which means high agreements. (Korean J Community Nutr 16(2) : 195~205, 2011)

The nutritional balance of the menu plans prepared according to the target pattern was examined. Total of 81 home economics teachers throughout the nation and 161 3rd-grade middle-school girls in Chungnam area participated. The data was collected by questionnaire and analyzed by using SPSS WIN 12.0....