46 results on '"Wen-Qing Yang"'
Search Results
2. Data from CC-223, a Potent and Selective Inhibitor of mTOR Kinase: In Vitro and In Vivo Characterization
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Heather K. Raymon, Sabita Sankar, Peter Worland, Stacie S. Canan, Mehran F. Moghaddam, Brian E. Cathers, Zeen Tong, Wen Qing Yang, Tao Shi, Tam Tran, Sophie Perrin-Ninkovic, Sophie X. Peng, Samantha Richardson, Rene Bissonette, Rama Krishna Narla, Matt Hickman, Kamran Ghoreishi, Julius Apuy, Jingjing Zhao, Jim Leisten, James C. Gamez, Godrej Khambatta, Garrick Packard, Weiming Xu, Shuichan Xu, Kimberly E. Fultz, and Deborah S. Mortensen
- Abstract
mTOR is a serine/threonine kinase that regulates cell growth, metabolism, proliferation, and survival. mTOR complex-1 (mTORC1) and mTOR complex-2 (mTORC2) are critical mediators of the PI3K–AKT pathway, which is frequently mutated in many cancers, leading to hyperactivation of mTOR signaling. Although rapamycin analogues, allosteric inhibitors that target only the mTORC1 complex, have shown some clinical activity, it is hypothesized that mTOR kinase inhibitors, blocking both mTORC1 and mTORC2 signaling, will have expanded therapeutic potential. Here, we describe the preclinical characterization of CC-223. CC-223 is a potent, selective, and orally bioavailable inhibitor of mTOR kinase, demonstrating inhibition of mTORC1 (pS6RP and p4EBP1) and mTORC2 [pAKT(S473)] in cellular systems. Growth inhibitory activity was demonstrated in hematologic and solid tumor cell lines. mTOR kinase inhibition in cells, by CC-223, resulted in more complete inhibition of the mTOR pathway biomarkers and improved antiproliferative activity as compared with rapamycin. Growth inhibitory activity and apoptosis was demonstrated in a panel of hematologic cancer cell lines. Correlative analysis revealed that IRF4 expression level associates with resistance, whereas mTOR pathway activation seems to associate with sensitivity. Treatment with CC-223 afforded in vivo tumor biomarker inhibition in tumor-bearing mice, after a single oral dose. CC-223 exhibited dose-dependent tumor growth inhibition in multiple solid tumor xenografts. Significant inhibition of mTOR pathway markers pS6RP and pAKT in CC-223–treated tumors suggests that the observed antitumor activity of CC-223 was mediated through inhibition of both mTORC1 and mTORC2. CC-223 is currently in phase I clinical trials. Mol Cancer Ther; 14(6); 1295–305. ©2015 AACR.
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- 2023
3. Supplementary Figure 1 from Identification of Nuclear Export Inhibitors with Potent Anticancer Activity In vivo
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Sumati Murli, Pieter B.M.W.M. Timmermans, Darren A. Craig, Shannon L. Ward, Steven D. Dong, Wen Qing Yang, and Sarah C. Mutka
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Supplementary Figure 1 from Identification of Nuclear Export Inhibitors with Potent Anticancer Activity In vivo
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- 2023
4. Supplementary Figure 3 from Identification of Nuclear Export Inhibitors with Potent Anticancer Activity In vivo
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Sumati Murli, Pieter B.M.W.M. Timmermans, Darren A. Craig, Shannon L. Ward, Steven D. Dong, Wen Qing Yang, and Sarah C. Mutka
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Supplementary Figure 3 from Identification of Nuclear Export Inhibitors with Potent Anticancer Activity In vivo
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- 2023
5. Supplementary Methods and Materials from Identification of Nuclear Export Inhibitors with Potent Anticancer Activity In vivo
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Sumati Murli, Pieter B.M.W.M. Timmermans, Darren A. Craig, Shannon L. Ward, Steven D. Dong, Wen Qing Yang, and Sarah C. Mutka
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Supplementary Methods and Materials from Identification of Nuclear Export Inhibitors with Potent Anticancer Activity In vivo
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- 2023
6. Supplementary Figure 2 from Identification of Nuclear Export Inhibitors with Potent Anticancer Activity In vivo
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Sumati Murli, Pieter B.M.W.M. Timmermans, Darren A. Craig, Shannon L. Ward, Steven D. Dong, Wen Qing Yang, and Sarah C. Mutka
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Supplementary Figure 2 from Identification of Nuclear Export Inhibitors with Potent Anticancer Activity In vivo
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- 2023
7. Isorhynchophylline inhibits inflammatory responses in endothelial cells and macrophages through the NF-κB/NLRP3 signaling pathway
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Li-Hua Wang, Zheng-Wei Gu, Jie Li, Wen-Qing Yang, Yun-Lun Li, Dong-Mei Qi, Dan-Yang Wang, and Hai-Qiang Jiang
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Complementary and alternative medicine - Abstract
Background Atherosclerosis is a chronic inflammatory disease of arterial wall, which is closely related to inflammatory reaction. In this study, the anti-inflammatory effect of isorhynchophylline was studied by NF- κB / NLRP3 pathway. Methods (1) ApoE−/− mice were fed with high-fat diet to establish atherosclerotic model, while C57 with the same genetic background was fed with common diet as control group. Body weight was recorded and blood lipids were detected. The expression of NLRP3, NF-κB, IL-18 and Caspase-1 in aorta was detected by Western-Blot and PCR, and plaque formation was detected by HE and oil red O staining. (2) Lipopolysaccharide interfered with Human Umbilical Vein Endothelial Cells (HUVECs) and RAW264.7 to form inflammatory model, and was treated with isorhynchophylline. The expression of NLRP3, NF-κB, IL-18 and Caspase-1 in aorta was detected by Western-Blot and PCR, and the ability of cell migration was detected by Transwell and scratch test. Results (1) the expression of NLRP3, NF- κB, IL-18 and Caspase-1 in aorta of model group was higher than that of control group, and plaque formation was obvious. (2) the expressions of NLRP3, NF- κB, IL-18 and Caspase-1 in HUVECs and RAW264.7 model groups were higher than those in control group, while isorhynchophylline decreased their expression and enhanced cell migration ability. Conclusion Isorhynchophylline can reduce the inflammatory reaction induced by lipopolysaccharide and promote the ability of cell migration.
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- 2023
8. Analysis of the Real-World Application Laws for Zhengan Xifeng Decoction in the Treatment of Hypertension Based on Machine Learning
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Jia-Ming Huan, Dan-Yang Cai, Jie Li, Zhen Hua, Xiao-Qing Chen, Wen-Qing Yang, Yuan-Long Hu, Yi-Fei Wang, and Yun-Lun Li
- Abstract
Hypertension is a common cardiovascular disease. Zhengan Xifeng Decoction (ZGXFD), a classic prescription for adjuvant treatment of hypertension, but its clinical application characteristics and biological information have not been comprehensively analysed. This study is based on real-world data from 7571 electronic medical records of hypertension patients treated by ZGXFD. The Apriori algorithm was used to obtain the coupled herbs of ZGXFD. A convolutional neural network was designed to measure the dose characteristic information of herbs. The topological features of the protein‒protein interaction data were used to analyse the biological information of herbs. The K-nearest neighbour model integrates the above characteristics of herbs into the same framework to observe the composition adjustment laws and mechanism of ZGXFD from multiple dimensions. Eighty-seven coupled herbs with dose characteristics were obtained. The results revealed that ZXGFD regulates cytokines and reduces the inflammatory response and metabolic disorder to achieve the purpose of adjuvant therapy. Moreover, machine learning model is used to analyze real-world data that include clinical and molecular biological data with hierarchical characteristics, which provides a micro-biological explanation for the clinical application of herbs.
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- 2023
9. Research on Source Range Channel Disturbance Diagnosis of Nuclear Instrumentation System
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Chao Wang, Xiao-Fei Li, Jing Xiao, Jun-Wei Zheng, Wen-Qing Yang, Zheng-Dong Huang, Sheng-Xin Yuan, and Ji-Kun Liu
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- 2023
10. Study on High Flux at Shutdown Alarm Setpoint Automatic Update Logic
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Wen-Qing Yang, Xiao-Fei Li, Wen-Qian Liu, Chao Wang, Bo Lv, Ji-Kun Liu, and Hao Luo
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- 2023
11. Analysis and Treatment on Control Rod Measured Position Fluctuation
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Wen-Qing Yang, Xiong-Wei Cheng, Chao Wang, Chu-Hao Huang, Zheng-Dong Huang, Ji-Kun Liu, and Qiao Wei
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- 2023
12. THUMPD3–TRMT112 is a m2G methyltransferase working on a broad range of tRNA substrates
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Jian-Yang Ge, Jing Li, Wen-Qing Yang, Hao Li, Yan Nie, Qing-Ping Xiong, Huan Lin, Ru-Juan Liu, Daizhu Lv, Wen-Yu Zhu, and Xiuying Lin
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chemistry.chemical_classification ,tRNA Methyltransferases ,Methyltransferase ,AcademicSubjects/SCI00010 ,Protein subunit ,RNA-Binding Proteins ,RNA ,Methyltransferases ,Biology ,Methylation ,Reverse genetics ,Substrate Specificity ,HEK293 Cells ,Enzyme ,RNA, Transfer ,chemistry ,Biochemistry ,Transfer RNA ,RNA and RNA-protein complexes ,Genetics ,Protein biosynthesis ,Humans ,RNA Processing, Post-Transcriptional ,Biogenesis ,HeLa Cells - Abstract
Post-transcriptional modifications affect tRNA biology and are closely associated with human diseases. However, progress on the functional analysis of tRNA modifications in metazoans has been slow because of the difficulty in identifying modifying enzymes. For example, the biogenesis and function of the prevalent N2-methylguanosine (m2G) at the sixth position of tRNAs in eukaryotes has long remained enigmatic. Herein, using a reverse genetics approach coupled with RNA-mass spectrometry, we identified that THUMP domain-containing protein 3 (THUMPD3) is responsible for tRNA: m2G6 formation in human cells. However, THUMPD3 alone could not modify tRNAs. Instead, multifunctional methyltransferase subunit TRM112-like protein (TRMT112) interacts with THUMPD3 to activate its methyltransferase activity. In the in vitro enzymatic assay system, THUMPD3–TRMT112 could methylate all the 26 tested G6-containing human cytoplasmic tRNAs by recognizing the characteristic 3′-CCA of mature tRNAs. We also showed that m2G7 of tRNATrp was introduced by THUMPD3–TRMT112. Furthermore, THUMPD3 is widely expressed in mouse tissues, with an extremely high level in the testis. THUMPD3-knockout cells exhibited impaired global protein synthesis and reduced growth. Our data highlight the significance of the tRNA: m2G6/7 modification and pave a way for further studies of the role of m2G in sperm tRNA derived fragments.
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- 2021
13. PS-C19-6: STUDY ON THE EFFICACY AND SAFETY OF TENG FU JIANGYA TABLET IN THE TREATMENT OF HYPERTENSION
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Wen qing Yang, Yun lun Li, Yu Wang, Yu Shuo Zhu, and Yu Chen Qi
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Physiology ,Internal Medicine ,Cardiology and Cardiovascular Medicine - Published
- 2023
14. A large area 3He tube array detector with vacuum operation capacity for the SANS instrument at the CSNS
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Xing-Fen Jiang, Jian-Rong Zhou, Hong Luo, Liang Xiao, Xiao-Juan Zhou, Hong Xu, Yuan-Guang Xia, Xiao-Guang Wu, Lin Zhu, Wen-Qing Yang, Gui-An Yang, Bei-Ju Guan, Hong-Yu Zhang, Yu-Bin Zhao, Zhi-Jia Sun, and Yuan-Bo Chen
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Nuclear and High Energy Physics ,Nuclear Energy and Engineering - Published
- 2022
15. The
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Yang, Wu, Yong-Zheng, Zhang, Meng-Jia, Li, Wen-Qing, Yang, and Lu-Feng, Cheng
- Abstract
Glioma is an aggressive tumor, currently there is no satisfactory management available. Psoralen, as a natural product, has been found to have an effect of treating cancer in recent years, but its effect on glioma has not been explored. In this study, we investigated the in vitro inhibition effect and potential targets of psoralen on glioma through network pharmacology and in
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- 2022
16. The occurrence order and cross-talk of different tRNA modifications
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Wen-Yu Zhu, Ru-Juan Liu, Cai-Tao Li, Jing Li, and Wen-Qing Yang
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0301 basic medicine ,Chemistry ,RNA methylation ,RNA ,Cooperativity ,Modified nucleosides ,General Biochemistry, Genetics and Molecular Biology ,Cell Physiological Phenomena ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Order (biology) ,RNA, Transfer ,Biochemistry ,Protein Biosynthesis ,030220 oncology & carcinogenesis ,RNA modification ,Transfer RNA ,Protein biosynthesis ,Humans ,RNA Processing, Post-Transcriptional ,General Agricultural and Biological Sciences ,General Environmental Science - Abstract
Chemical modifications expand the composition of RNA molecules from four standard nucleosides to over 160 modified nucleosides, which greatly increase the complexity and utility of RNAs. Transfer RNAs (tRNAs) are the most heavily modified cellular RNA molecules and contain the largest variety of modifications. Modification of tRNAs is pivotal for protein synthesis and also precisely regulates the noncanonical functions of tRNAs. Defects in tRNA modifications lead to numerous human diseases. Up to now, more than 100 types of modifications have been found in tRNAs. Intriguingly, some modifications occur widely on all tRNAs, while others only occur on a subgroup of tRNAs or even only a specific tRNA. The modification frequency of each tRNA is approximately 7% to 25%, with 5-20 modification sites present on each tRNA. The occurrence and modulation of tRNA modifications are specifically noticeable as plenty of interplays among different sites and modifications have been discovered. In particular, tRNA modifications are responsive to environmental changes, indicating their dynamic and highly organized nature. In this review, we summarized the known occurrence order, cross-talk, and cooperativity of tRNA modifications.
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- 2021
17. Triage by PAX1 and ZNF582 Methylation in Women With Cervical Intraepithelial Neoplasia Grade 3: A Multicenter Case–Control Study
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Kun Fu, Ming Lei, Li-Sha Wu, Jing-Cheng Shi, Si-Yu Yang, Wen-Qing Yang, Jin-Yun Xu, Ya-Nan Kang, Zhen-Ying Yang, Xuan Zhang, Kang-Ni Huang, Chi Han, Yan Tian, and Yu Zhang
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Infectious Diseases ,Oncology - Abstract
Background The colposcopy-conization inconsistency is common in women with cervical intraepithelial neoplasia grade 3 (CIN3). No adequate method has been reported to identify the final pathology of conization. In this study, we explored the ability of PAX1 and ZNF582 methylation to predict the pathological outcome of conization in advance. Methods This was a multicenter study and included 277 histologically confirmed CIN3 women who underwent cold knife conization (CKC) from January 2019 to December 2020. The methylation levels of PAX1 (PAX1m) and ZNF582 (ZNF582m) were determined by quantitative methylation-specific polymerase chain reaction (qMSP) and expressed in ΔCp. Receiver operating characteristic curves were used to evaluate predictive accuracy. Results The final pathological results in 48 (17.33%) patients were inflammation or low-grade squamous intraepithelial lesion (LSIL), 190 (68.59%) were high-grade squamous intraepithelial lesion (HSIL), and 39 (14.08%) were squamous cervical cancer (SCC). PAX1m and ZNF582m increased as lesions progressed from inflammation/LSIL, HSIL, to SCC. PAX1 and ZNF582 methylation yielded better prediction performance compared with common screening strategies, whether individually or combined. A 4.33-fold increase in the probability of inflammation/LSIL was observed in patients with lower ZNF582 methylation levels (ΔCpZNF582 ≥ 19.18). A 6.53-fold increase in SCC risk was observed in patients with elevated ZNF582 methylation (ΔCpZNF582 < 7.09). Conclusions DNA methylation would be an alternative screening method to triage and predict the final outcome of conization in CIN3 cases.
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- 2022
18. Analysis and Treatment of Accidental Drop of Shutdown Bank
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Chao Wang, Wen-Qing Yang, Zheng-Dong Huang, Xiao-Fei Li, Sheng-Xin Yuan, Dong-Liang Liu, Yu Wang, Xiong-Wei Cheng, and Jing Xiao
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- 2022
19. A dual role of human tRNA methyltransferase hTrmt13 in regulating translation and transcription
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Wen-Qing Yang, Han Dong, Qing-Ping Xiong, Zhi-Xuan Huang, Beisi Xu, Jing Li, En-Duo Wang, Ru-Juan Liu, Cai-Tao Li, Qi-Yu Zeng, and Hao Li
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Zinc finger ,tRNA Methyltransferases ,General Immunology and Microbiology ,General Neuroscience ,TRNA Methyltransferase ,RNA-Binding Proteins ,Translation (biology) ,Biology ,Methylation ,General Biochemistry, Genetics and Molecular Biology ,Chromatin ,Cell biology ,chemistry.chemical_compound ,RNA, Transfer ,chemistry ,Transcription (biology) ,Transfer RNA ,Transcriptional regulation ,Humans ,RNA ,RNA Processing, Post-Transcriptional ,Molecular Biology ,DNA - Abstract
Since numerous RNAs and RBPs prevalently localize to active chromatin regions, many RNA-binding proteins (RBPs) may be potential transcriptional regulators. RBPs are generally thought to regulate transcription via noncoding RNAs. Here, we describe a distinct, dual mechanism of transcriptional regulation by the previously uncharacterized tRNA-modifying enzyme, hTrmt13. On one hand, hTrmt13 acts in the cytoplasm to catalyze 2'-O-methylation of tRNAs, thus regulating translation in a manner depending on its tRNA-modification activity. On the other hand, nucleus-localized hTrmt13 directly binds DNA as a transcriptional co-activator of key epithelial-mesenchymal transition factors, thereby promoting cell migration independent of tRNA-modification activity. These dual functions of hTrmt13 are mutually exclusive, as it can bind either DNA or tRNA through its CHHC zinc finger domain. Finally, we find that hTrmt13 expression is tightly associated with poor prognosis and survival in diverse cancer patients. Our discovery of the noncatalytic roles of an RNA-modifying enzyme provides a new perspective for understanding epitranscriptomic regulation.
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- 2021
20. ThermomiR-377-3p-induced Suppression of Cirbp Expression Is Required for Effective Elimination of Cancer Cells and Cancer Stem-like Cells by Hyperthermia
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Yong-Long Li, Yan Sun, Chen Zhou, Hong-Fen Shen, Jie Yang, Jia-Wei Xia, Xiao-Lin Lin, Lin-Bei Chen, Weiren Luo, Rong Xiao-Xiang, Tao-Yan Lin, Sheng-Jun Xiao, Jun-Shuang Jia, Yei Le, Qi-Wen Li, Zhi-Zhi Yang, Xiao-Jun Luo, Wen-Qing Yang, Fang Wei, Zhi-Hao Zhou, Shu-Jun Lin, Dong Xiao, Wenhua Huang, Bing-Xia Zhao, and Shu-Wen Xue
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Hyperthermia ,business.industry ,Cancer cell ,Cancer research ,medicine ,Cancer ,medicine.disease ,business ,CIRBP - Abstract
In recent years, the development of adjunctive therapeutic hyperthermia for cancer therapy has received considerable attention. However, the mechanisms underlying hyperthermia resistance are still poorly understood. In this study, we investigated the roles of cold-inducible RNA binding protein (Cirbp) in regulating hyperthermia resistance and underlying mechanisms in nasopharyngeal carcinoma (NPC). Our results firstly revealed that hyperthermia significantly attenuated the stemness property of NPC cells, while combination treatment of hyperthermia and oridonin dramatically increased the killing effect on NPC cells and cancer stem cell (CSC)-like population. Moreover, hyperthermia substantially improved the sensitivity of radiation-resistant NPC cells and CSC-like cells to radiotherapy. Hyperthermia noticeably suppressed Cirbp expression in NPC cells and xenograft tumor tissues. Furthermore, Cirbp inhibition remarkably boosted anti-tumor-killing activity of hyperthermia against NPC cells and CSC-like cells, whereas ectopic expression of Cirbp compromised tumor-killing effect of hyperthermia on these cells, indicating that Cirbp overexpression induces hyperthermia resistance. ThermomiR-377–3p improved the sensitivity of NPC cells and cancer stem-like cells to hyperthermia in vitro by directly suppressing Cirbp expression. More importantly, our results displayed the significantly boosted sensitization of tumor xenografts to hyperthermia by Cirbp silencing in vivo, but ectopic expression of Cirbp nearly completely counteracted hyperthermia-mediated tumor cell-killing effect against tumor xenografts in vivo. Mechanistically, Cirbp silencing-induced inhibition of DNA damage repair by inactivating ATM-Chk2 and ATR-Chk1 pathways, decrease in stemness and increase in cell death contributed to hyperthermic sensitization; conversely, Cirbp overexpression-induced promotion of DNA damage repair, increase in stemness and decrease in cell apoptosis contributed to hyperthermia resistance. Taken together, these findings reveal a previously unrecognized role for Cirbp in positively regulating hyperthermia resistance and suggest that thermomiR-377–3p and its target gene Cirbp represent promising targets for therapeutic hyperthermia.
- Published
- 2021
21. [Non-targeted metabolomics study and biomarker screening of prehypertensive liver-fire hyperactivity syndrome based on UPLC-Q-Exactive MS technology]
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Yu, Wang, Shu-Min, Li, Chao, Li, Wen-Qing, Yang, and Yun-Lun, Li
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Technology ,Liver ,Humans ,Metabolomics ,Syndrome ,Biomarkers ,Chromatography, High Pressure Liquid - Abstract
In this study, patients with prehypertensive liver-fire hyperactivity syndrome(LFHS) were selected as the research objects. The plasma samples of healthy volunteers and patients with prehypertensive LFHS were analyzed by non-targeted metabolomics based on UPLC-Q-Exactive MS. The differential biomarkers and metabolic pathways were screened out by multivariate statistics and metabolic pathway analysis, which revealed the characteristics of metabolic patterns of the syndrome. Thirty-three potential biomarkers such as androsterone and lysophosphatidylcholine and 16 related metabolic pathways such as steroid hormone metabolism and lipid metabolism were identified, and a partial least squares-discriminant analysis(PLS-DA) model of traditional Chinese medicine(TCM) syndromes was preliminarily constructed: Y =-0.070X_(13)-0.006X_8+ 0.040X_5-0.152X_1+0.131X_(10)+0.036X_(11)+0.043X_(23)+0.076X_(16)+0.132X_(20)+0.081X_(19)-0.101X_(31)+0.082X_(15)-0.038X_9+0.079X_(24). The predictive value of the model was 88.1%, and the explanatory power was 88.4%. In this study, the characteristic metabolic pattern of the prehypertensive LFHS was distinguished and revealed by metabolomics. The constructed PLS-DA model is expected to provide an objective basis for the identification of TCM syndromes in prehypertension, and inspiration for exploring the biological basis of TCM syndromes at small-molecular and overall levels.
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- 2021
22. Helicase POLQ-like (HELQ) as a novel indicator of platinum-based chemoresistance for epithelial ovarian cancer
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Si-Yu Yang, Ji-Ye Yin, Gang Yin, Yong-Bin Liu, Yu Zhang, Kun Fu, Wen-Qing Yang, Yan Tian, Jun-You Zhu, Pei-Yao Li, and Jing Long
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0301 basic medicine ,DNA Repair ,endocrine system diseases ,DNA repair ,Antineoplastic Agents ,Carcinoma, Ovarian Epithelial ,Disease-Free Survival ,03 medical and health sciences ,0302 clinical medicine ,Western blot ,RNA interference ,Cell Line, Tumor ,medicine ,Humans ,Neoplasms, Glandular and Epithelial ,Neoplasm Staging ,Ovarian Neoplasms ,Cisplatin ,Gene knockdown ,biology ,medicine.diagnostic_test ,business.industry ,DNA Helicases ,Obstetrics and Gynecology ,Helicase ,Middle Aged ,Immunohistochemistry ,Survival Rate ,Reverse transcription polymerase chain reaction ,030104 developmental biology ,Oncology ,Drug Resistance, Neoplasm ,Gene Knockdown Techniques ,030220 oncology & carcinogenesis ,biology.protein ,Cancer research ,Female ,business ,medicine.drug ,Nucleotide excision repair - Abstract
Objective To investigate the role of HELQ in chemo-resistance of epithelial ovarian carcinoma (EOC), which is a critical factor of patients' prognosis. Methods Immunohistochemistry, survival analysis of our 87 EOC patients and bioinformatics analysis of The Cancer Genome Atlas (TCGA) datasets (Nature, 2011) disclosed the clinical importance of HELQ expression. Quantitative reverse transcription polymerase chain reaction (qRT-PCR), and Western Blot analyses of EOC tissue were used to confirm it. Ectopic overexpression and RNA interference knockdown of HELQ were carried out in OVCAR3 and A2780 cell lines, respectively, to determine the effect of altered HELQ expression on cellular response to cisplatin by CCK8 assay. The DNA repair capacity of these cells was evaluated by using host-cell reactivation assay. Western Blot analyses were carried out to determine the effect of HLEQ on the DNA repair genes by using cells with altered HELQ expression. Results HELQ expression associates with response of EOC patients to platinum-based chemotherapy and their overall survival (OS), disease free survival (DFS). HELQ overexpression or knockdown, respectively, increased and decreased the cellular resistance to cisplatin, DNA repair activity, and expression of DNA repair proteins of Nucleotide excision repair (NER) pathway. Conclusions HELQ plays an important role in regulating the expression of DNA repair proteins NER pathway which, in turn, contributes to cellular response to cisplatin and patients' response to platinum-based chemotherapy. Our results demonstrated that HELQ could serve as a novel indicator for chemo-resistance of EOC, which can predict the prognosis of the disease.
- Published
- 2018
23. CC-223, a Potent and Selective Inhibitor of mTOR Kinase: In Vitro and In Vivo Characterization
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Jingjing Zhao, Sophie X. Peng, Brian E. Cathers, Sabita Sankar, Garrick Packard, Stacie S. Canan, Shuichan Xu, Heather Raymon, Godrej Khambatta, Rama K. Narla, Kimberly Elizabeth Fultz, Sophie Perrin-Ninkovic, Matt Hickman, Tam Tran, Samantha J. Richardson, Jim Leisten, Kamran Ghoreishi, Rene Bissonette, Mehran F. Moghaddam, Julius Apuy, Tao Shi, Deborah Mortensen, Wen Qing Yang, Weiming Xu, James C. Gamez, Peter Worland, and Zeen Tong
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Cancer Research ,Blotting, Western ,Apoptosis ,P70-S6 Kinase 1 ,Mechanistic Target of Rapamycin Complex 2 ,Mice, SCID ,mTORC1 ,Mechanistic Target of Rapamycin Complex 1 ,Biology ,mTORC2 ,In vivo ,Cell Line, Tumor ,Neoplasms ,Animals ,Humans ,Protein Kinase Inhibitors ,PI3K/AKT/mTOR pathway ,Cell Proliferation ,Dose-Response Relationship, Drug ,Molecular Structure ,Neovascularization, Pathologic ,Cell growth ,Kinase ,TOR Serine-Threonine Kinases ,RPTOR ,HCT116 Cells ,Xenograft Model Antitumor Assays ,Tumor Burden ,HEK293 Cells ,Oncology ,Multiprotein Complexes ,Pyrazines ,Cancer research ,Female - Abstract
mTOR is a serine/threonine kinase that regulates cell growth, metabolism, proliferation, and survival. mTOR complex-1 (mTORC1) and mTOR complex-2 (mTORC2) are critical mediators of the PI3K–AKT pathway, which is frequently mutated in many cancers, leading to hyperactivation of mTOR signaling. Although rapamycin analogues, allosteric inhibitors that target only the mTORC1 complex, have shown some clinical activity, it is hypothesized that mTOR kinase inhibitors, blocking both mTORC1 and mTORC2 signaling, will have expanded therapeutic potential. Here, we describe the preclinical characterization of CC-223. CC-223 is a potent, selective, and orally bioavailable inhibitor of mTOR kinase, demonstrating inhibition of mTORC1 (pS6RP and p4EBP1) and mTORC2 [pAKT(S473)] in cellular systems. Growth inhibitory activity was demonstrated in hematologic and solid tumor cell lines. mTOR kinase inhibition in cells, by CC-223, resulted in more complete inhibition of the mTOR pathway biomarkers and improved antiproliferative activity as compared with rapamycin. Growth inhibitory activity and apoptosis was demonstrated in a panel of hematologic cancer cell lines. Correlative analysis revealed that IRF4 expression level associates with resistance, whereas mTOR pathway activation seems to associate with sensitivity. Treatment with CC-223 afforded in vivo tumor biomarker inhibition in tumor-bearing mice, after a single oral dose. CC-223 exhibited dose-dependent tumor growth inhibition in multiple solid tumor xenografts. Significant inhibition of mTOR pathway markers pS6RP and pAKT in CC-223–treated tumors suggests that the observed antitumor activity of CC-223 was mediated through inhibition of both mTORC1 and mTORC2. CC-223 is currently in phase I clinical trials. Mol Cancer Ther; 14(6); 1295–305. ©2015 AACR.
- Published
- 2015
24. Kibdelosporangium metalli sp. nov., isolated from a rare earth mine
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Yan-Ru Cao, Ya Dao, Lian-Ming Liang, Zhong-Kan He, Wen-Qing Yang, and Bin Hu
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0301 basic medicine ,DNA, Bacterial ,China ,Diamino acid ,Peptidoglycan ,Biology ,Diaminopimelic Acid ,Microbiology ,Mining ,03 medical and health sciences ,chemistry.chemical_compound ,RNA, Ribosomal, 16S ,Actinomycetales ,Gene ,Ecology, Evolution, Behavior and Systematics ,Phospholipids ,Phylogeny ,Soil Microbiology ,Phosphatidylglycerol ,chemistry.chemical_classification ,Strain (chemistry) ,Fatty Acids ,Fatty acid ,Vitamin K 2 ,General Medicine ,Sequence Analysis, DNA ,16S ribosomal RNA ,Bacterial Typing Techniques ,030104 developmental biology ,chemistry ,Biochemistry ,DNA - Abstract
A Gram-stain-positive, oxidase-negative, catalase-positive isolate forming sporangium-like globular bodies, isolated from the rare earth mine of Bayan Obo in China and designated strain KC 266T, was subjected to a comprehensive taxonomic study. Comparative 16S rRNA gene sequence analysis revealed that strain KC 266T represented a novel lineage within the genus Kibdelosporangium and showed highest 16S rRNA gene similarities to Kibdelosporangium philippinense (98.5 %), Kibdelosporangium aridum subsp. largum (98.2 %), Kibdelosporangium aridum subsp. aridum (98.2 %) and Kibdelosporangium phytohabitans (98.0 %). The DNA–DNA relatedness between strain KC 266T and the four species of the genus Kibdelosporangium was less than 60 %. The DNA G+C content of strain KC 266T was 67.9 mol%. The quinone system consisted of major amounts of MK-9(H4) and MK-9(H2), minor amounts of MK-8(H2) and traces of MK-10(H4). The diamino acid of the peptidoglycan was meso-diaminopimelic acid. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylinositol, two unknown phospholipids and one unidentified aminophospholipid. The major cellular fatty acids were iso-C16 : 0, C17 : 1 ω6c, iso-C15 : 0 and iso-C14 : 0. Physiological traits as well as unique traits of the polar lipid profile and the fatty acid pattern distinguished strain KC 266T from the most closely related species. All these results indicate that strain KC 266T represents a novel species of the genus Kibdelosporangium , for which the name Kibdelosporangium metalli sp. nov. is proposed. The type strain is KC 266T (=KCTC 39719T=CCTCC AA 2016002T).
- Published
- 2016
25. Research on the Frequency Dynamic Characteristics and UFLS Scheme in Shanghai Subzone Power Grid
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Wen Qing Yang, Zhi Gang Ding, and Wei Cao
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Scheme (programming language) ,Engineering ,business.industry ,Load Shedding ,Ring network ,General Medicine ,Grid ,Power (physics) ,Peak load ,Electronic engineering ,Power grid ,Transient (oscillation) ,business ,computer ,computer.programming_language - Abstract
Shanghai main power grid is operating in 500 kV ring network and with voltage-grading & district-dividing structure, and its subzone power grids have their own frequency characteristics. The paper has used district-dividing single equivalent model to check the parameters of components in BPA transient simulation program, and the original under frequency load shedding (UFLS) scheme was improved. Then the frequency dynamic characteristics of isolated subzone grid is analyzed, so does the adaptability and frequency safety of power grid under the new UFLS scheme in the condition of peak load in the summer of 2011.
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- 2013
26. Research on the Technology of Converting the Existing AC Lines to DC Lines
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Wen Qing Yang, Lin Chen, Wei Cao, and Jian Kun Wu
- Subjects
Power transmission ,Engineering ,business.industry ,General Engineering ,Electrical engineering ,Power (physics) ,Electric power system ,Electric power transmission ,Transmission (telecommunications) ,Transmission line ,HVAC ,Electronic engineering ,HVDC converter station ,business - Abstract
Power transmission is a key link in power system. As the increase of power supply, the transmission capacity of the lines should be enlarged too. In the developed area, the right-of-way for transmission line is hard to be obtained. And converting the existing HVAC overhead transmission lines using HVDC technology could enhance the transmission capability. There are three possible plans for different HVAC transmission lines: single-pole HVDC, bi-pole HVDC and tri-pole HVDC.
- Published
- 2012
27. [The mechanism of action of valsartan studied by HPLC-TOF/MS]
- Author
-
Wen-qing, Yang, Yun-lun, Li, and Hai-qiang, Jiang
- Subjects
Rats, Inbred SHR ,Metabolome ,Animals ,Discriminant Analysis ,Metabolomics ,Valsartan ,Blood Pressure ,Least-Squares Analysis ,Biomarkers ,Chromatography, High Pressure Liquid ,Mass Spectrometry ,Rats - Abstract
High performance liquid chromatography-time-off-flight mass spectrometer (HPLC-TOFMS) technology coupled with partial least squares discriminant analysis (PLS-DA) processed by SIMCA-P software was applied to investigate serum endogenous metabolites alternations of valsartan in spontaneous hypertension rats (SHR). And MetPA platform was used to connect identified potential biomarkers in corresponding metabolic pathways to find possible therapeutic mechanism of valsartan. Valsartan significantly declined the blood pressure of SHRs (P0.05) at fourth week. The metabolic profiling significantly changed and four metabolites involved in G protein-coupled pathway were identified. Metabolomics is able to detect holistic and microcosmic alternations in organism, so as to elucidate therapeutic mechanism of drugs.
- Published
- 2015
28. Aerodynamic Mechanism Research of Flapping Flight
- Author
-
Zhan Ke Li, Bi Feng Song, Wen Qing Yang, Wenping Song, and Ya Feng Zhang
- Subjects
Physics ,Airfoil ,animal structures ,Angle of attack ,business.industry ,General Engineering ,Lift (soaring) ,Thrust ,Aerodynamics ,eye diseases ,Vortex ,body regions ,Vortex lift ,Flapping ,Aerospace engineering ,business ,human activities - Abstract
Lift makes a vehicle in air and thrust makes advancing. The lift of flapping wing air vehicle is composed of two main parts, flapping lift and advancing lift. The advancing lift of flapping-wing is similar as of fixed-wing, generated mainly by relative velocity and angle of attack. The flapping lift is owned only by flapping wing. The flapping lift is generated by asymmetry flapping motion manner of wings, asymmetry airfoil, and asymmetry folding in flapping cycle, accordingly leading-edge vortex and wake capture effect. The thrust is completely generated by flapping wing and the magnitude of thrust is mainly controlled by flapping frequency and flapping manner. The flapping motion is a thrust generator and lift enhancing manner. Flapping wing air vehicle will be one of the star members of man-made air vehicles.
- Published
- 2011
29. Computational Fluid-Structure Dynamics Coupling Research of Flexible Wing
- Author
-
Zhan Ke Li, Ya Feng Zhang, Wen Qing Yang, Wenping Song, and Bi Feng Song
- Subjects
Coupling ,Engineering ,Wing ,business.industry ,General Engineering ,Thrust ,Structural engineering ,Aerodynamics ,Computational fluid dynamics ,GeneralLiterature_MISCELLANEOUS ,Lift (force) ,Wing twist ,business ,Reynolds-averaged Navier–Stokes equations ,ComputingMethodologies_COMPUTERGRAPHICS - Abstract
This paper presents a computational fluid-structure dynamics coupling method of flexible wing. The computational fluid dynamics is solving RANS equations. The stiffness used in computational structure dynamics is tested by experiments. Then the aerodynamic performance of flexible wing is researched and compared with rigid wing. The results show that the lift and thrust generated by flexible wing are less than the rigid wing. Flexible wing can slower the variety of aerodynamic performance when the flight environment is changing. And flexible wing has bigger stalling angle.
- Published
- 2011
30. A Comparison of Short-Circuit Current Decaying Calculation between PSS/E BKDY and Computational Curve
- Author
-
Jing Tian Ge, Wei Cao, Wen Qing Yang, Zhi Gang Ding, and Xiao Qing Qi
- Subjects
Dynamic simulation ,Mathematical optimization ,General Engineering ,Applied mathematics ,Current (fluid) ,Short circuit ,Mathematics - Abstract
The calculations of short-circuit current decrement with PSS/E BKDY and computational curve were compared with the one by the dynamic simulation of PSS/E. Calculation shows that the accuracy of the result by PSS/E BKDY is quite good while the one by computational curve is so conservative that it makes larger errors. The reasons for the above results have been analyzed theoretically.
- Published
- 2011
31. Non-equilibrium transformation kinetics and primary grain size distribution in the rapid solidification of Fe–B hypereutectic alloy
- Author
-
Baiping Lu, Wen Qing Yang, F. Liu, Han-Hui Wang, and Gaolin Yang
- Subjects
Phase transition ,Materials science ,Mechanical Engineering ,Alloy ,Metals and Alloys ,Nucleation ,Thermodynamics ,engineering.material ,Microstructure ,Residual ,Transformation (function) ,Mechanics of Materials ,Scientific method ,Particle-size distribution ,Materials Chemistry ,engineering - Abstract
Considering nucleation, growth and impingement, a numerical model was proposed to describe the transformation kinetics of rapid solidification in bulk undercooled Fe–B hypereutectic alloy. Applying heat balance and solute conservation, the changes of temperature and composition of residual liquid are proved to be not only dependent of time, but also associated with the solidified fraction during the transformation. Accordingly, the driving force for non-equilibrium solidification, as well as the determined nucleation and growth rates, decreases with the process of transformation. On this basis, the primary grain size distribution formed in this process can be predicted with the combination of classical Kolmogorov–Johnson–Mehl–Avrami (KJMA) equation and a probability statistic analysis. Good agreement between the model prediction and experimental results has shown the validity of the proposed model.
- Published
- 2011
32. Description of Recalescence in Bulk Undercooled Alloys
- Author
-
Feng Liu, Wen Qing Yang, and Gencang Yang
- Subjects
Materials science ,Alloy ,General Engineering ,Intermetallic ,Recalescence ,Thermodynamics ,engineering.material ,Residual ,Gibbs free energy ,symbols.namesake ,Thermal ,Flow balance ,symbols ,engineering ,Solid solution - Abstract
Assuming phenomenological thermal flow balance and solute conservation, a numerical model was proposed for recalescence in bulk undercooled alloy. During the calculation, the evolution of residual melt temperature and concentration is solved as function of the transformed solid fraction. After quantitative thermodynamic calculations, a transition from non-equilibrium to equilibrium process can be described by the change of liquid/solid Gibbs energy difference. The proposed model has been applied to Cu-Ni solid solution and Fe-B hypereutectic alloy. The predicted post-recalescence temperature TR agrees well with the experimental results.
- Published
- 2010
33. Identification of Nuclear Export Inhibitors with Potent Anticancer Activity In vivo
- Author
-
Wen Qing Yang, Sarah C. Mutka, Pieter Timmermans, Sumati Murli, Darren A. Craig, Steven D. Dong, and Shannon L. Ward
- Subjects
Cancer Research ,Active Transport, Cell Nucleus ,Mice, Nude ,Receptors, Cytoplasmic and Nuclear ,Apoptosis ,Bone Neoplasms ,Karyopherins ,Biology ,Article ,Mice ,XPO1 ,In vivo ,Cell Line, Tumor ,Animals ,Humans ,Nuclear export signal ,Lung ,Cell Nucleus ,Osteosarcoma ,Antibiotics, Antineoplastic ,Cell Cycle ,Fibroblasts ,Cell cycle ,HCT116 Cells ,Xenograft Model Antitumor Assays ,In vitro ,Mice, Inbred C57BL ,Oncology ,Biochemistry ,Cell culture ,Cancer cell ,Fatty Acids, Unsaturated ,Cancer research ,Female ,Tumor Suppressor Protein p53 - Abstract
The export protein CRM1 is required for the nuclear export of a wide variety of cancer-related “cargo” proteins including p53, c-Abl, and FOXO-3A. Leptomycin B (LMB) is a highly specific inhibitor of CRM1 with significant in vitro potency but limited in vivo efficacy due to toxicity. We now report a series of semisynthetic LMB derivatives showing substantially improved therapeutic windows. Exposure of cancer cells to these compounds leads to a rapid and prolonged block of nuclear export and apoptosis. In contrast to what is observed in cancer cells, these agents induce cell cycle arrest, but not apoptosis, in normal lung fibroblasts. These new nuclear export inhibitors (NEI) maintain the high potency of LMB, are up to 16-fold better tolerated than LMB in vivo, and show significant efficacy in multiple mouse xenograft models. These NEIs show the potential of CRM1 inhibitors as novel and potent anticancer agents. [Cancer Res 2009;69(2):510–7]
- Published
- 2009
34. Researches on Rules of the Longitudinal Residual Stress Distribution in Straightening Deformation Zone of Heavy Rail with Multi-Rollers
- Author
-
Yong Ming Wang, Ping Yi Wang, Zhong Liang Tian, Yong Qiang Duan, Lin Chen, Zhang Zhong Wu, Jian-guo Wang, Mi Chao Gao, Wei Zong, and Wen Qing Yang
- Subjects
Materials science ,Finite element software ,business.industry ,Mechanical Engineering ,Base (geometry) ,Structural engineering ,Deformation (meteorology) ,Condensed Matter Physics ,Finite element method ,Mechanics of Materials ,Residual stress ,Head (vessel) ,General Materials Science ,business - Abstract
The paper simulated and researched the straightening process of heavy rail by finite element software of ANSYS/LS-DYNA. The residual stress of the rail head, rail base, rail loin in the 7th deformation zone meets the real straightening condition in the straightening simulation. The calculation indicates: The residual stress of the rail head, rail base, rail loin in various deformation zones varies significantly like the variation of tensile-compression-tensile. Compared with the on-site rule, the residual stress of rail base decreased155 Mpa, this is in agreement with the values of practice.
- Published
- 2008
35. Efficacy and Safety Evaluation of Human Reovirus Type 3 in Immunocompetent Animals
- Author
-
D A Stewart, Patrick W.K. Lee, Huong Muzik, Matthew C. Coffey, Wen Qing Yang, Penny M. A. Brasher, Brad Thompson, Mark G. Hamilton, Richard H. Dyck, Xueqing Lun, Cheryl A. Palmer, N. Berry Rewcastle, David George, Donna L. Senger, Peter A. Forsyth, Kevin Fonseca, Zhong Qiao Shi, Randal N. Johnston, Sandra Nishikawa, and M. Elizabeth Wilcox
- Subjects
Cancer Research ,Pathology ,medicine.medical_specialty ,biology ,viruses ,virus diseases ,Reoviridae ,biochemical phenomena, metabolism, and nutrition ,medicine.disease ,biology.organism_classification ,Virology ,Virus ,Immunoglobulin G ,Oncology ,Glioma ,Cancer cell ,medicine ,biology.protein ,Viral shedding ,Viral load ,Encephalitis - Abstract
Purpose: Human reovirus type 3 has been proposed to kill cancer cells with an activated Ras signaling pathway. The purpose of this study was to investigate the efficacy of reovirus in immunocompetent glioma animal models and safety/toxicity in immunocompetent animals, including nonhuman primates. Experimental Design: Racine glioma cells 9L and RG2 were implanted s.c. or intracranially in Fisher 344 rats with or without reovirus antibodies, followed by treatment of reovirus. To study whether reovirus kills contralateral tumors in the brain and to determine viral distribution, we established an in situ dual tumor model followed by reovirus intratumoral inoculation only into the ipsilateral tumor. To evaluate neurotoxicity/safety of reovirus, Cynomolgus monkeys and immunocompetent rats were given intracranially with reovirus, and pathological examination and/or behavioral studies were done. Viral shedding and clinical biochemistry were systematically studied in monkeys. Results: Intratumorally given reovirus significantly suppressed the growth of both s.c. and intracranially tumors and significantly prolonged survival. The presence of reovirus-neutralizing antibodies did not abort the reovirus’ antitumor effect. Reovirus inhibited glioma growth intracranially in the ipsilateral but not the contralateral tumors; viral load in ipsilateral tumors was 15 to 330-fold higher than the contralateral tumors. No encephalitis or behavioral abnormalities were found in monkeys and rats given reovirus intracranially. No treatment-related clinical biochemistry changes or diffuse histopathological abnormality were found in monkeys inoculated intracranially with Good Manufacturing Practice prepared reovirus. Microscopic changes were confined to the region of viral inoculation and were dose related, suggesting reovirus intracranially was well tolerated in nonhuman primates. Conclusions: These data show the efficacy and safety of reovirus when it is used in the treatment of gliomas in immunocompetent hosts. Inoculation of reovirus into the brain of nonhuman primates did not produce significant toxicities.
- Published
- 2004
36. Reovirus as an experimental therapeutic for brain and leptomeningeal metastases from breast cancer
- Author
-
Xueqing Lun, Wen Qing Yang, D A Stewart, Donna L. Senger, Penny M. A. Brasher, Peter A. Forsyth, David George, N B Rewcastle, Richard H. Dyck, Kara L. Norman, Huong Muzik, Patrick W. K. Lee, and Zhong-Qiao Shi
- Subjects
Pathology ,medicine.medical_specialty ,viruses ,Genetic enhancement ,Green Fluorescent Proteins ,Mice, Nude ,Breast Neoplasms ,Inflammation ,Injections, Intralesional ,Transfection ,Metastasis ,Lethal Dose 50 ,Mice ,Breast cancer ,In vivo ,Cell Line, Tumor ,Meningeal Neoplasms ,Genetics ,medicine ,Animals ,Humans ,Mammalian orthoreovirus 3 ,Molecular Biology ,Cell Death ,Brain Neoplasms ,business.industry ,Neoplasms, Experimental ,medicine.disease ,Effective dose (pharmacology) ,Reoviridae Infections ,Models, Animal ,Molecular Medicine ,Female ,medicine.symptom ,business ,Encephalitis ,Brain metastasis - Abstract
Brain and leptomeningeal metastases are common in breast cancer patients and our current treatments are ineffective. Reovirus type 3 is a replication competent, naturally occurring virus that usurps the activated Ras-signaling pathway (or an element thereof) of tumor cells and lyses them but leaves normal cells relatively unaffected. In this study we evaluated reovirus as an experimental therapeutic in models of central nervous system (CNS) metastasis from breast cancer. We found all breast cancer cell lines tested were susceptible to reovirus, with > 50% of these cells lysed within 72 h of infection. In vivo neurotoxicity studies showed only mild local inflammation at the injection site and mild communicating hydrocephalus with neither diffuse encephalitis nor behavioral abnormalities at the therapeutically effective dose of reovirus (intracranial) (ie 10(7) plaque-forming units) or one dose level higher. In vivo, a single intratumoral administration of reovirus significantly reduced the size of tumors established from two human breast cancer cell lines and significantly prolonged survival. Intrathecal administration of reovirus also remarkably prolonged survival in an immunocompetent racine model of leptomeningeal metastases. These data suggest that the evaluation of reovirus as an experimental therapeutic for CNS metastases from breast cancer is warranted.
- Published
- 2004
37. Application of bundle care for gastrointestinal functional recovery in patients with severe acute pancreatitis
- Author
-
Xue Li, Wen-Qing Yang, and Yi-Xia Zhou
- Subjects
medicine.medical_specialty ,business.industry ,Bundle ,Medicine ,Acute pancreatitis ,In patient ,business ,medicine.disease ,Intensive care medicine ,Functional recovery - Published
- 2017
38. [Intervention effects of qingre jiangya capsule on brain hippocampus of spontaneously hypertensive rats based on metabonomic research]
- Author
-
Hai-Qing, Jiang, Lei, Nie, Yun-Lun, Li, Miao-Miao, Wang, Mei, Zhu, Wen-Qing, Yang, and Xin-Ya, Zhang
- Subjects
Male ,Rats, Inbred SHR ,Hypertension ,Animals ,Capsules ,Rats, Wistar ,Hippocampus ,Drugs, Chinese Herbal ,Rats - Abstract
Thirty SHRs were obtained randomly to hypertension, model group, captopril group and Qingre jiangya capsule group. Ten Wistar rats were used as control group. The hippocampus tissue was removed to explore the damage of spontaneously hypertensive rats (SHR) and the protective effect of Qingre jiangya capsule after continuously administered for 14 days. And then the data were processed by principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA). The research results revealed captopril group was significantly different from the other three groups. The classification of other three groups is also very clear after captopril group removed. This suggested that Qingre jiangya capsule could improve the overall metabolism compared with captopril. Four metabolites were identified: dimethylglycine, glycerophosphocholine, aldosterone and noradrenaline. Hypertension hippocampus damage may mainly be expressed in tyrosine metabolism, aldosterone-regulated sodium, vascular smooth muscle contraction reabsorption, and Qingre jiangya capsule could reverse the hippocampus tissue damage of SHR.
- Published
- 2014
39. Immunohistochemical observation of macrophage colony stimulating factor and its receptor in breast cancer and hepatoma tissues
- Author
-
Yu-Hua Song, Dexian Zheng, Wen-qing Yang, Yong-Min Lin, Ge Li, and Ke-Fu Wu
- Subjects
Macrophage colony-stimulating factor ,Cancer Research ,Pathology ,medicine.medical_specialty ,Fetus ,medicine.drug_class ,Hyperplasia ,Biology ,medicine.disease ,Monoclonal antibody ,Breast cancer ,Oncology ,medicine ,Immunohistochemistry ,Macrophage ,Receptor - Abstract
Objective: To study the potential role of cellular macrophage colony-stimulating factor (cM-CSF) and cellular macrophage colony-stimulating factor receptor (cM-CSF-R) with breast cancer and hepatoma and search the way for clinical application. Methods: Frozen surgical specimens from 48 breast cancer patients, including 29 cases of histological grade II and 19 eases of grade III, and 16 hepatoma patients were investigated by Avidin Biotin Complex (ABC) immunohistochemical assay with anti-M-CSF monoclonal antibody (Mab) and anti-M-CSF-R Mab. Pathohistological examination was performed as well. Results: cM-CSF and cM-CSF-R were detected in tested specimens. The expression levels of cM-CSF and cM-CSF-R in grade III group were higher than in grade II group and more higher than control group hyperplasia of breast. Hepatoma tissues also showed higher expression level of cM-CSF and cM-CSF-R than normal adult and fetal liver. Conclusion: Breast cancer and hepatoma tissues presented higher expression levels of cM-CSF and cM-CSF-R than control and expression level might be related with tumor’s process.
- Published
- 2001
40. Co-expression of macrophage colony-stimulating factor with its receptor in human hepatoma cells and its potential roles
- Author
-
Lina Zhang, Yuhua Song, Naiguo Song, Minghe Zhao, Ke-Fu Wu, Lusong Zhang, and Wen-qing Yang
- Subjects
Macrophage colony-stimulating factor ,Cancer Research ,medicine.medical_specialty ,Biology ,medicine.disease ,Molecular biology ,Peripheral blood mononuclear cell ,digestive system diseases ,Reverse transcription polymerase chain reaction ,Endocrinology ,Oncology ,Internal medicine ,Hepatocellular carcinoma ,medicine ,Immunohistochemistry ,Macrophage ,Autocrine signalling ,Receptor - Abstract
Objective: To investigate the potential role of macrophage colony-stimulating factor (M-CSF) and macrophage colony-stimulating factor receptor (M-CSF-R) on the growth of human hepatoma cells. Methods: Specimens of different origin, including tissues of human hepatocellular carcinoma (HCC), human fetal liver (FL) and normal liver (NL), the hepatoma cell lines, as well as the peripheral blood mononuclear cells (PBMC) from patients with HCC or liver metastatic tumor (LMT), were used to detect the expression levels of M-CSF and M-CSF-R by ABC immunohistochemistry staining and reverse transcription polymerase chain reaction methods the expression levels of M-CSF and M-CSF-R. Influence of monoclonal antibody against M-CSF (B5) or M-CSF-R (RE2) on proliferation ability of hepatoma cell linesin vitro was also studied. Results: The results showed that hepatoma tissues produced elevated levels of both M-CSF and M-CSF-R compared with those of fetal liver (P
- Published
- 1999
41. Pulsed electromagnetic wave exposure induces ultrastructural damage and upregulated expression of heat shock protein 70 in the rat adenohypophysis
- Author
-
Gui‑Ying Zeng, Kang Cheng, Jun Yi, Xiao‑Feng Huang, Xiao‑Guang Zhou, Yong‑Qiang Li, Dong‑Qing Ren, Wen‑Qing Yang, and Yong‑Bin Chen
- Subjects
Male ,Cancer Research ,Necrosis ,Immunocytochemistry ,Biology ,Biochemistry ,Andrology ,Rats, Sprague-Dawley ,Downregulation and upregulation ,Pituitary Gland, Anterior ,Genetics ,medicine ,Animals ,HSP70 Heat-Shock Proteins ,Molecular Biology ,Mitochondrial vacuolation ,Electromagnetic Radiation ,Molecular biology ,Immunohistochemistry ,Hsp70 ,Rats ,Up-Regulation ,Oncology ,Apoptosis ,Ultrastructure ,Molecular Medicine ,medicine.symptom ,Whole body - Abstract
The aim of the present study was to investigate the ultrastructural damage and the expression of heat shock protein 70 (HSP70) in the rat adenohypophysis following pulsed electromagnetic wave (PEMW) exposure. The rats were randomly divided into four groups: Sham PEMW exposure, 1 x 10(4) pulses of PEMW exposure, 1 x 10(5) pulses of PEMW exposure and 3 x 10(5) pulses of PEMW exposure. Whole body radiation of 1 x 10(4) pulses, 1 x 10(5) pulses and 3 x 10(5) pulses of PEMW were delivered with a field strength of 100 kV/m. The rats in each group (n=6 in each) were sacrificed 12, 24, 48 and 96 h after PEMW exposure. Transmission electron microscopy was then used to detect the ultrastructural changes and immunocytochemistry was used to examine the expression of HSP70. Cellular damage, including mitochondrial vacuolation occurred as early as 12 h after PEMW exposure.More severe cellular damages, including cell degeneration and necrosis, occurred 24 and 48 h after PEMW exposure. The PEMW-induced cellular damage increased as the number of PEMW pulses increased. In addition, the expression of HSP70 significantly increased following PEMW exposure and peaked after 12 h. These findings suggested that PEMW induced ultrastructural damages in the rat adenohypophysis and that HSP70 may have contributed to the PEMW-induced adenohypophyseal damage.
- Published
- 2013
42. [Effect of naringenin on learning and memory ability on model rats with Alzheimer disease]
- Author
-
Jing, Ma, Wen-Qing, Yang, He, Zha, and Hua-Rong, Yu
- Subjects
Male ,Time Factors ,Superoxide Dismutase ,Brain ,tau Proteins ,Antioxidants ,Rats ,Rats, Sprague-Dawley ,Disease Models, Animal ,Oxidative Stress ,Alzheimer Disease ,Memory ,Malondialdehyde ,Flavanones ,Animals ,Learning ,Maze Learning ,Rutaceae ,Drugs, Chinese Herbal - Abstract
To investigate the effects of naringenin on the learning and memory ability of Alzheimer disease (AD) rats.30 male SD rats were randomly divided into control group (sham operation group), model group and naringenin group. AD model was established by injecting strepoztocin (3 mg/kg) twice into each of two intracerebroventriculas. Naringenin group were given intragastric administration of naringenin once a day for 3 weeks and the other two groups were given intragadtric administration of normal saline with the same dosage and time period. After 3 weeks, learning and memory ability in all the three groups were analyzed by Morris water maze, the activity of superoxide dismutase(SOD) and the content of malondialdehyde (MDA) of the rats' brain tissue was detected by chemical colorimetric determination. Observed the expressions of Abeta42 and Abeta40 by immunohistochemical method. The expression and degree of phosphorylation of tau protein was assayed by western blotting.1. Compared with the sham operation group, the mean escape latency of the model group was significantly prolonged (P0.05) and the time that rats were in the platform quadrant was significantly shortened (P0.0.5). On the contrary, compared with the model group, the mean escape latency of naringenin group was significantly shortened (P0.05) and the time that rats were in the platform quadrant was significantly extended (P0.005). 2. The level of MDA in the model group, compared with the sham operation group group, was significantly increased (P0.05). Whereas, that of naringenin group, compared with the model group, was significantly decreased compared with the sham operation group (P0.05). The activity of SOD in the naringenin group was significantly increased comparing with the model group (P0.05). 3. The expressions of Abeta40 and Abeta42 in model group were obviously up-regulated. Instead, the expressions of Abeta40 and Abeta42 in the naringenin group were significantly down regulated. 4. There was no significant difference in the expression of tau protein among each groups. Nevertheless, the phosphorylation of tau protein in the model group was significantly enhanced than that in the control group (P0.05), and the phosphorylation of tau protein in the naringenin group was significantly reduced than that in the model group (P0.05).Naringenin can improve learning and memory ability of model rats with Alzheimer disease through the approach of oxidative stress.
- Published
- 2013
43. Evaluation of Improvement Effect of Restorer Lines on Pyramiding Genes Re-sistant to Rice Blast, Bacterial Leaf Blight and Brown Planthopper
- Author
-
Jian-Long Xu, Tian-Kuan Luo, Zhong-Xing Li, Jue Lou, Zheng Guochu, Yue Gaohong, Hua-Jin Lu, Yong-Chu Xie, and Wen-Qing Yang
- Subjects
Agronomy ,Blight ,Plant Science ,Brown planthopper ,Biology ,biology.organism_classification ,Agronomy and Crop Science ,Gene ,Biotechnology - Published
- 2016
44. Efficacy and safety evaluation of human reovirus type 3 in immunocompetent animals: racine and nonhuman primates
- Author
-
Wen Qing, Yang, Xueqing, Lun, Cheryl Ann, Palmer, M Elizabeth, Wilcox, Huong, Muzik, Zhong Qiao, Shi, Richard, Dyck, Matt, Coffey, Brad, Thompson, Mark, Hamilton, Sandra G, Nishikawa, Penny M A, Brasher, Kevin, Fonseca, David, George, N Berry, Rewcastle, Randal N, Johnston, Doug, Stewart, Patrick W K, Lee, Donna L, Senger, and Peter A, Forsyth
- Subjects
Male ,Brain Neoplasms ,Reverse Transcriptase Polymerase Chain Reaction ,Green Fluorescent Proteins ,Rats, Inbred F344 ,Rats ,Survival Rate ,Macaca fascicularis ,Rats, Nude ,Neutralization Tests ,Immunoglobulin G ,Models, Animal ,Tumor Cells, Cultured ,Animals ,Encephalitis ,Humans ,Female ,Glioblastoma ,Maze Learning ,Mammalian orthoreovirus 3 ,In Situ Hybridization - Abstract
Human reovirus type 3 has been proposed to kill cancer cells with an activated Ras signaling pathway. The purpose of this study was to investigate the efficacy of reovirus in immunocompetent glioma animal models and safety/toxicity in immunocompetent animals, including nonhuman primates.Racine glioma cells 9L and RG2 were implanted s.c. or intracranially in Fisher 344 rats with or without reovirus antibodies, followed by treatment of reovirus. To study whether reovirus kills contralateral tumors in the brain and to determine viral distribution, we established an in situ dual tumor model followed by reovirus intratumoral inoculation only into the ipsilateral tumor. To evaluate neurotoxicity/safety of reovirus, Cynomolgus monkeys and immunocompetent rats were given intracranially with reovirus, and pathological examination and/or behavioral studies were done. Viral shedding and clinical biochemistry were systematically studied in monkeys.Intratumorally given reovirus significantly suppressed the growth of both s.c. and intracranially tumors and significantly prolonged survival. The presence of reovirus-neutralizing antibodies did not abort the reovirus' antitumor effect. Reovirus inhibited glioma growth intracranially in the ipsilateral but not the contralateral tumors; viral load in ipsilateral tumors was 15 to 330-fold higher than the contralateral tumors. No encephalitis or behavioral abnormalities were found in monkeys and rats given reovirus intracranially. No treatment-related clinical biochemistry changes or diffuse histopathological abnormality were found in monkeys inoculated intracranially with Good Manufacturing Practice prepared reovirus. Microscopic changes were confined to the region of viral inoculation and were dose related, suggesting reovirus intracranially was well tolerated in nonhuman primates.These data show the efficacy and safety of reovirus when it is used in the treatment of gliomas in immunocompetent hosts. Inoculation of reovirus into the brain of nonhuman primates did not produce significant toxicities.
- Published
- 2004
45. Reovirus prolongs survival and reduces the frequency of spinal and leptomeningeal metastases from medulloblastoma
- Author
-
Wen Qing, Yang, Donna, Senger, Huong, Muzik, Zhong Qiao, Shi, Denise, Johnson, Penny M A, Brasher, N Barry, Rewcastle, Mark, Hamilton, Jim, Rutka, Johannes, Wolff, Cynthia, Wetmore, Tom, Curran, Patrick W K, Lee, and Peter A, Forsyth
- Subjects
Transcription, Genetic ,Eukaryotic Initiation Factor-2 ,Green Fluorescent Proteins ,Mice, Nude ,Virus Replication ,Drug Administration Schedule ,Proto-Oncogene Proteins p21(ras) ,Mice ,eIF-2 Kinase ,Genes, Reporter ,Meningeal Neoplasms ,Tumor Cells, Cultured ,Animals ,Humans ,Spinal Cord Neoplasms ,Cerebellar Neoplasms ,Mammalian orthoreovirus 3 ,Injections, Spinal ,Genes, p53 ,Xenograft Model Antitumor Assays ,Neoplasm Proteins ,Biological Therapy ,Enzyme Activation ,Luminescent Proteins ,Female ,Medulloblastoma ,Signal Transduction - Abstract
Medulloblastoma (MB), the most common pediatric brain tumor, is a highly malignant disease with a 5-year survival rate of only 60%. Tumor cells invade surrounding tissue and disseminate through cerebral spinal fluid, making treatment difficult. Human reovirus type 3 exploits an activated Ras pathway in tumor cells to support productive infection as an oncolytic virus. Here, we examined the ability of human reovirus to kill MB cells lines and surgical specimens in vitro and inhibit tumor growth/metastases in vivo. Most human MB cell lines tested (five of seven = 71.4%), two MB cell lines derived from spontaneously arising tumors in Patched-1(+/-) mice (two of two = 100%) and three MB primary cultures derived from surgical specimens, were susceptible to reovirus infection. Reovirus was internalized and transcribed in both susceptible and resistant cell lines. However, viral protein synthesis was restricted to cell lines with higher levels of activated Ras, suggesting that Ras plays a critical role in reovirus oncolysis in MB. Using an in vivo Daoy orthotopic animal model, we found that a single i.t. injection of reovirus dramatically prolonged survival compared with controls (160 versus 70 days, respectively; P = 0.0003). Repeating this experiment with GFP-labeled Daoy cells and multiple i.t. administrations of reovirus, we again found prolonged survival and a dramatic reduction in spinal and leptomeningeal metastases (66.7% in control injections versus 0.0% in the live virus group). These data suggest that this oncolytic virus may be a potentially effective novel therapy against human MB. Its ability to reduce metastases to the spinal cord could allow a reduction in the dose/field of total neuroaxis cerebral-spinal radiotherapy currently used to treat/prevent cerebral spinal fluid dissemination.
- Published
- 2003
46. Preparation and characterization of Tl 2 Ba 2 Ca 2 Cu 3 O x superconducting thin films
- Author
-
Wen-Qing Yang, Dequan Yue, F. T. Chan, Wei Luo, Ning Huang, Gregory J. Salamo, Yongan Tang, Xiangfu Zong, Huan Sheng Cheng, and Z.Z. Sheng
- Subjects
Laser ablation ,Materials science ,Excimer laser ,Phase (matter) ,medicine.medical_treatment ,X-ray crystallography ,Analytical chemistry ,medicine ,Substrate (electronics) ,Thin film ,Dispersion (chemistry) ,Secondary electrons - Abstract
Superconducting Tl-Ba-Ca-Cu-O thin films have been fabricated by laser ablation. There are two steps in this process. First, an excimer laser is focused onto the Ba-Ca-Cu-O target forming a Ba-Ca-Cu-O plume. These particles are then deposited on substrate; second, the precursor films are placed into quartz tube to anneal in the presence of unfired Tl2Ba2Ca2Cu3O10 pellet to form the superconducting phase. Zero-resistance temperature Tc and critical current density Jc of the best film were 12 Ik and 1 X 106A/cm2 (at 77 K) respectively. X-ray diffraction (XRD) and secondary electron microscope (SEM) have been used to study the crystalline structure, while the composition are analyzed by high energy ion backscattering and checked with energy dispersion X-ray analysis (EDAX) and secondary ion mass spectroscopy (SIMS). Results show that these superconducting films are predominately composed of the (2223) phase and highly oriented. A platelet structure plays an important role on the quality of film.© (1994) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
- Published
- 1994
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