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Co-expression of macrophage colony-stimulating factor with its receptor in human hepatoma cells and its potential roles
- Source :
- Chinese Journal of Cancer Research. 11:79-84
- Publication Year :
- 1999
- Publisher :
- Chinese Journal of Cancer Research, 1999.
-
Abstract
- Objective: To investigate the potential role of macrophage colony-stimulating factor (M-CSF) and macrophage colony-stimulating factor receptor (M-CSF-R) on the growth of human hepatoma cells. Methods: Specimens of different origin, including tissues of human hepatocellular carcinoma (HCC), human fetal liver (FL) and normal liver (NL), the hepatoma cell lines, as well as the peripheral blood mononuclear cells (PBMC) from patients with HCC or liver metastatic tumor (LMT), were used to detect the expression levels of M-CSF and M-CSF-R by ABC immunohistochemistry staining and reverse transcription polymerase chain reaction methods the expression levels of M-CSF and M-CSF-R. Influence of monoclonal antibody against M-CSF (B5) or M-CSF-R (RE2) on proliferation ability of hepatoma cell linesin vitro was also studied. Results: The results showed that hepatoma tissues produced elevated levels of both M-CSF and M-CSF-R compared with those of fetal liver (P
- Subjects :
- Macrophage colony-stimulating factor
Cancer Research
medicine.medical_specialty
Biology
medicine.disease
Molecular biology
Peripheral blood mononuclear cell
digestive system diseases
Reverse transcription polymerase chain reaction
Endocrinology
Oncology
Internal medicine
Hepatocellular carcinoma
medicine
Immunohistochemistry
Macrophage
Autocrine signalling
Receptor
Subjects
Details
- ISSN :
- 19930631 and 10009604
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Chinese Journal of Cancer Research
- Accession number :
- edsair.doi...........8d10343f82e40c285c0e2ad27142d924