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A dual role of human tRNA methyltransferase hTrmt13 in regulating translation and transcription
- Source :
- The EMBO Journal. 41
- Publication Year :
- 2021
- Publisher :
- EMBO, 2021.
-
Abstract
- Since numerous RNAs and RBPs prevalently localize to active chromatin regions, many RNA-binding proteins (RBPs) may be potential transcriptional regulators. RBPs are generally thought to regulate transcription via noncoding RNAs. Here, we describe a distinct, dual mechanism of transcriptional regulation by the previously uncharacterized tRNA-modifying enzyme, hTrmt13. On one hand, hTrmt13 acts in the cytoplasm to catalyze 2'-O-methylation of tRNAs, thus regulating translation in a manner depending on its tRNA-modification activity. On the other hand, nucleus-localized hTrmt13 directly binds DNA as a transcriptional co-activator of key epithelial-mesenchymal transition factors, thereby promoting cell migration independent of tRNA-modification activity. These dual functions of hTrmt13 are mutually exclusive, as it can bind either DNA or tRNA through its CHHC zinc finger domain. Finally, we find that hTrmt13 expression is tightly associated with poor prognosis and survival in diverse cancer patients. Our discovery of the noncatalytic roles of an RNA-modifying enzyme provides a new perspective for understanding epitranscriptomic regulation.
- Subjects :
- Zinc finger
tRNA Methyltransferases
General Immunology and Microbiology
General Neuroscience
TRNA Methyltransferase
RNA-Binding Proteins
Translation (biology)
Biology
Methylation
General Biochemistry, Genetics and Molecular Biology
Chromatin
Cell biology
chemistry.chemical_compound
RNA, Transfer
chemistry
Transcription (biology)
Transfer RNA
Transcriptional regulation
Humans
RNA
RNA Processing, Post-Transcriptional
Molecular Biology
DNA
Subjects
Details
- ISSN :
- 14602075 and 02614189
- Volume :
- 41
- Database :
- OpenAIRE
- Journal :
- The EMBO Journal
- Accession number :
- edsair.doi.dedup.....70d47e38fd97cfb12de39ef9e9466cdd