91 results on '"Melinda T. Coughlan"'
Search Results
2. Valproic acid attenuates cellular senescence in diabetic kidney disease through the inhibition of complement C5a receptors
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Melinda T. Coughlan, Mark Ziemann, Adrienne Laskowski, Trent M. Woodruff, and Sih Min Tan
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Histone Deacetylase Inhibitors ,Mice ,Multidisciplinary ,Valproic Acid ,Animals ,Diabetic Nephropathies ,Complement C5a ,Receptor, Anaphylatoxin C5a ,Cellular Senescence ,Diabetes Mellitus, Experimental - Abstract
Despite increasing knowledge about the factors involved in the progression of diabetic complications, diabetic kidney disease (DKD) continues to be a major health burden. Current therapies only slow but do not prevent the progression of DKD. Thus, there is an urgent need to develop novel therapy to halt the progression of DKD and improve disease prognosis. In our preclinical study where we administered a histone deacetylase (HDAC) inhibitor, valproic acid, to streptozotocin-induced diabetic mice, albuminuria and glomerulosclerosis were attenuated. Furthermore, we discovered that valproic acid attenuated diabetes-induced upregulation of complement C5a receptors, with a concomitant reduction in markers of cellular senescence and senescence-associated secretory phenotype. Interestingly, further examination of mice lacking the C5a receptor 1 (C5aR1) gene revealed that cellular senescence was attenuated in diabetes. Similar results were observed in diabetic mice treated with a C5aR1 inhibitor, PMX53. RNA-sequencing analyses showed that PMX53 significantly regulated genes associated with cell cycle pathways leading to cellular senescence. Collectively, these results for the first time demonstrated that complement C5a mediates cellular senescence in diabetic kidney disease. Cellular senescence has been implicated in the pathogenesis of diabetic kidney disease, thus therapies to inhibit cellular senescence such as complement inhibitors present as a novel therapeutic option to treat diabetic kidney disease.
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- 2022
3. Novel Therapies for Kidney Disease in People With Diabetes
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Richard J MacIsaac, Elif I Ekinci, Nayana Khurana, Melinda T. Coughlan, and Steven James
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Oncology ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,Clinical Biochemistry ,Type 2 diabetes ,Incretins ,Biochemistry ,Endocrinology ,Internal medicine ,Diabetes mellitus ,medicine ,Animals ,Humans ,Diabetic Nephropathies ,Bardoxolone methyl ,Adverse effect ,Sodium-Glucose Transporter 2 Inhibitors ,business.industry ,Biochemistry (medical) ,Atrasentan ,Prognosis ,medicine.disease ,Clinical trial ,Diabetes Mellitus, Type 2 ,Albuminuria ,medicine.symptom ,business ,medicine.drug ,Kidney disease - Abstract
Context The increasing burden of diabetic kidney disease (DKD) has led to the discovery of novel therapies. Objective This review aims to summarize the results of recent clinical trials that test the efficacy of potential therapies for DKD. Methods A systematized narrative review was performed utilizing the PubMed, Embase (Ovid), CINAHL, and Cochrane databases (January 2010 to January 2021). The included trials assessed the efficacy of specific medications using renal endpoints in adult participants with type 1 or 2 diabetes. Results Fifty-three trials were identified. Large, multinational, and high-powered trials investigating sodium-glucose cotransporter 2 (SGLT2) inhibitors demonstrated improved renal outcomes, even in patients with established DKD. Trials examining incretin-related therapies also showed some improvement in renal outcomes. Additionally, mineralocorticoid receptor antagonists exhibited potential with multiple improved renal outcomes in large trials, including those involving participants with established DKD. Atrasentan, baricitinib, ASP8232, PF-04634817, CCX140-B, atorvastatin, fenofibrate, probucol, doxycycline, vitamin D, omega-3 fatty acids, silymarin, turmeric, total glucosides of paeony, and tripterygium wilfordii Hook F extract were all associated with some improved renal endpoints but need further exploration. While bardoxolone methyl was associated with a decrease in albuminuria, high rates of cardiovascular adverse effects curtailed further exploration into this agent. Selonsertib, allopurinol, praliciguat, palosuran, benfotiamine, and diacerein were not associated with improved renal outcomes. Conclusion Trials have yielded promising results in the search for new therapies to manage DKD. SGLT2 inhibitors and incretin-related therapies have demonstrated benefit and were associated with improved cardiovascular outcomes. Mineralocorticoid receptor antagonists are another class of agents with increasing evidence of benefits.
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- 2021
4. Therapeutic Potential of Targeting Complement C5a Receptors in Diabetic Kidney Disease
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Inez A. Trambas, Melinda T. Coughlan, and Sih Min Tan
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Inorganic Chemistry ,Organic Chemistry ,General Medicine ,Physical and Theoretical Chemistry ,Molecular Biology ,Spectroscopy ,Catalysis ,Computer Science Applications - Abstract
Diabetic kidney disease (DKD) affects 30–40% of patients with diabetes and is currently the leading cause of end-stage renal disease (ESRD). The activation of the complement cascade, a highly conserved element of the innate immune system, has been implicated in the pathogenesis of diabetes and its complications. The potent anaphylatoxin C5a is a critical effector of complement-mediated inflammation. Excessive activation of the C5a-signalling axis promotes a potent inflammatory environment and is associated with mitochondrial dysfunction, inflammasome activation, and the production of reactive oxygen species. Conventional renoprotective agents used in the treatment of diabetes do not target the complement system. Mounting preclinical evidence indicates that inhibition of the complement system may prove protective in DKD by reducing inflammation and fibrosis. Targeting the C5a-receptor signaling axis is of particular interest, as inhibition at this level attenuates inflammation while preserving the critical immunological defense functions of the complement system. In this review, the important role of the C5a/C5a-receptor axis in the pathogenesis of diabetes and kidney injuries will be discussed, and an overview of the status and mechanisms of action of current complement therapeutics in development will be provided.
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- 2023
5. Review of potential biomarkers of inflammation and kidney injury in diabetic kidney disease
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Vuthi Khanijou, Neda Zafari, Melinda T. Coughlan, Richard J. MacIsaac, and Elif I. Ekinci
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Inflammation ,Endocrinology ,Lipocalin-2 ,Endocrinology, Diabetes and Metabolism ,Internal Medicine ,Diabetes Mellitus ,Albuminuria ,Humans ,Diabetic Nephropathies ,Hepatitis A Virus Cellular Receptor 1 ,Kidney ,Biomarkers ,Glomerular Filtration Rate - Abstract
Diabetic kidney disease is expected to increase rapidly over the coming decades with rising prevalence of diabetes worldwide. Current measures of kidney function based on albuminuria and estimated glomerular filtration rate do not accurately stratify and predict individuals at risk of declining kidney function in diabetes. As a result, recent attention has turned towards identifying and assessing the utility of biomarkers in diabetic kidney disease. This review explores the current literature on biomarkers of inflammation and kidney injury focussing on studies of single or multiple biomarkers between January 2014 and February 2020. Multiple serum and urine biomarkers of inflammation and kidney injury have demonstrated significant association with the development and progression of diabetic kidney disease. Of the inflammatory biomarkers, tumour necrosis factor receptor-1 and -2 were frequently studied and appear to hold most promise as markers of diabetic kidney disease. With regards to kidney injury biomarkers, studies have largely targeted markers of tubular injury of which kidney injury molecule-1, beta-2-microglobulin and neutrophil gelatinase-associated lipocalin emerged as potential candidates. Finally, the use of a small panel of selective biomarkers appears to perform just as well as a panel of multiple biomarkers for predicting kidney function decline.
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- 2022
6. Deficiency of Prebiotic Fiber and Insufficient Signaling Through Gut Metabolite-Sensing Receptors Leads to Cardiovascular Disease
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Matthew Snelson, Gavin W. Lambert, Assam El-Osta, Grant R Drummond, D. Donner, Melinda T. Coughlan, Helen Kiriazis, Aanthony Vinh, Duncan Horlock, Charles R. Mackay, Waled A. Shihata, Hamdi A. Jama, Beverly Giam, Tim D. Spector, Chad Johnson, Mark Ziemann, Xiao-Jun Du, April Fiedler, Kirill Tsyganov, Francine Z. Marques, David M. Kaye, Sarah E. Phillips, Amrita Vijay, and Ana M. Valdes
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Dietary Fiber ,Male ,medicine.medical_specialty ,medicine.medical_treatment ,Metabolite ,Disease ,030204 cardiovascular system & hematology ,Receptors, G-Protein-Coupled ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Intestinal mucosa ,Physiology (medical) ,Internal medicine ,medicine ,Animals ,Intestinal Mucosa ,Risk factor ,Receptor ,030304 developmental biology ,Mice, Knockout ,0303 health sciences ,business.industry ,Prebiotic ,Fatty Acids, Volatile ,Gastrointestinal Microbiome ,Prebiotics ,Blood pressure ,Endocrinology ,chemistry ,Hypertension ,Signal transduction ,Cardiology and Cardiovascular Medicine ,business ,Signal Transduction - Abstract
Background: High blood pressure (BP) continues to be a major, poorly controlled but modifiable risk factor for cardiovascular death. Among key Western lifestyle factors, a diet poor in fiber is associated with prevalence of high BP. The impact of lack of prebiotic fiber and the associated mechanisms that lead to higher BP are unknown. Here we show that lack of prebiotic dietary fiber leads to the development of a hypertensinogenic gut microbiota, hypertension and its complications, and demonstrate a role for G-protein coupled-receptors (GPCRs) that sense gut metabolites. Methods: One hundred seventy-nine mice including C57BL/6J, gnotobiotic C57BL/6J, and knockout strains for GPR41, GPR43, GPR109A, and GPR43/109A were included. C57BL/6J mice were implanted with minipumps containing saline or a slow-pressor dose of angiotensin II (0.25 mg·kg -1 ·d -1 ). Mice were fed diets lacking prebiotic fiber with or without addition of gut metabolites called short-chain fatty acids ([SCFA)] produced during fermentation of prebiotic fiber in the large intestine), or high prebiotic fiber diets. Cardiac histology and function, BP, sodium and potassium excretion, gut microbiome, flow cytometry, catecholamines and methylation-wide changes were determined. Results: Lack of prebiotic fiber predisposed mice to hypertension in the presence of a mild hypertensive stimulus, with resultant pathological cardiac remodeling. Transfer of a hypertensinogenic microbiota to gnotobiotic mice recapitulated the prebiotic-deprived hypertensive phenotype, including cardiac manifestations. Reintroduction of SCFAs to fiber-depleted mice had protective effects on the development of hypertension, cardiac hypertrophy, and fibrosis. The cardioprotective effect of SCFAs were mediated via the cognate SCFA receptors GPR43/GPR109A, and modulated L-3,4-dihydroxyphenylalanine levels and the abundance of T regulatory cells regulated by DNA methylation. Conclusions: The detrimental effects of low fiber Westernized diets may underlie hypertension, through deficient SCFA production and GPR43/109A signaling. Maintaining a healthy, SCFA-producing microbiota is important for cardiovascular health.
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- 2020
7. Intravascular Follistatin gene delivery improves glycemic control in a mouse model of type 2 diabetes
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Mark A. Febbraio, Darren C. Henstridge, Paul Gregorevic, Jonathan R. Davey, Hongwei Qian, Helen Ludlow, Mark P. Hedger, Martin Whitham, Melinda T. Coughlan, Sean L. McGee, Adam Hagg, Rachel E. Thomson, Emma Estevez, and Kevin I. Watt
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0301 basic medicine ,Follistatin ,endocrine system ,medicine.medical_specialty ,medicine.medical_treatment ,Glucose uptake ,Glycemic Control ,Type 2 diabetes ,Biochemistry ,Diabetes Mellitus, Experimental ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Insulin resistance ,Internal medicine ,Diabetes mellitus ,Genetics ,medicine ,Animals ,Molecular Biology ,Glycemic ,biology ,business.industry ,Insulin ,Gene Transfer Techniques ,Skeletal muscle ,Genetic Therapy ,medicine.disease ,030104 developmental biology ,Endocrinology ,medicine.anatomical_structure ,Diabetes Mellitus, Type 2 ,Hyperglycemia ,biology.protein ,Administration, Intravenous ,Insulin Resistance ,business ,hormones, hormone substitutes, and hormone antagonists ,030217 neurology & neurosurgery ,Biotechnology - Abstract
Type 2 diabetes (T2D) manifests from inadequate glucose control due to insulin resistance, hypoinsulinemia, and deteriorating pancreatic β-cell function. The pro-inflammatory factor Activin has been implicated as a positive correlate of severity in T2D patients, and as a negative regulator of glucose uptake by skeletal muscle, and of pancreatic β-cell phenotype in mice. Accordingly, we sought to determine whether intervention with the Activin antagonist Follistatin can ameliorate the diabetic pathology. Here, we report that an intravenous Follistatin gene delivery intervention with tropism for striated muscle reduced the serum concentrations of Activin B and improved glycemic control in the db/db mouse model of T2D. Treatment reversed the hyperglycemic progression with a corresponding reduction in the percentage of glycated-hemoglobin to levels similar to lean, healthy mice. Follistatin gene delivery promoted insulinemia and abundance of insulin-positive pancreatic β-cells, even when treatment was administered to mice with advanced diabetes, supporting a mechanism for improved glycemic control associated with maintenance of functional β-cells. Our data demonstrate that single-dose intravascular Follistatin gene delivery can ameliorate the diabetic progression and improve prognostic markers of disease. These findings are consistent with other observations of Activin-mediated mechanisms exerting deleterious effects in models of obesity and diabetes, and suggest that interventions that attenuate Activin signaling could help further understanding of T2D and the development of novel T2D therapeutics.
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- 2020
8. Exploring the role of the metabolite-sensing receptor GPR109a in diabetic nephropathy
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Matthew Snelson, Sih Min Tan, Runa S.J. Lindblom, Gavin C Higgins, and Melinda T. Coughlan
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Male ,0301 basic medicine ,medicine.medical_specialty ,food.ingredient ,Physiology ,Metabolite ,Pharmacology ,Permeability ,Diabetes Mellitus, Experimental ,Receptors, G-Protein-Coupled ,Diabetic nephropathy ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,food ,Internal medicine ,Diabetes mellitus ,Animals ,Medicine ,Diabetic Nephropathies ,Resistant starch ,Receptor ,Glycated Hemoglobin ,Mice, Knockout ,Type 1 diabetes ,Intestinal permeability ,business.industry ,Body Weight ,Dietary fibre ,Glomerulosclerosis ,Streptozotocin ,medicine.disease ,Intestines ,Endocrinology ,030104 developmental biology ,chemistry ,030220 oncology & carcinogenesis ,Albuminuria ,Dietary fiber ,medicine.symptom ,business ,Gut homeostasis ,medicine.drug - Abstract
Alterations in gut homeostasis may contribute to the progression of diabetic nephropathy. There has been recent attention on the renoprotective effects of metabolite-sensing receptors in chronic renal injury, including the G-protein-coupled-receptor (GPR)109a, which ligates the short chain fatty acid butyrate. However, the role of GPR109a in the development of diabetic nephropathy, a milieu of diminished microbiome-derived metabolites, has not yet been determined. This study aimed to assess the effects of insufficient GPR109a signalling via genetic deletion of GPR109a on the development of renal injury in diabetic nephropathy. Gpr109a−/− mice or their wildtype littermates (Gpr109a+/+) were rendered diabetic with streptozotocin (STZ). Mice received a control diet or an isocaloric high fiber diet (12.5% resistant starch) for 24 weeks and gastrointestinal permeability and renal injury were determined. Diabetes was associated with increased albuminuria, glomerulosclerosis and inflammation. In comparison, Gpr109a−/− mice with diabetes did not show an altered renal phenotype. Resistant starch supplementation did not afford protection from renal injury in diabetic nephropathy. Whilst diabetes was associated with alterations in intestinal morphology, intestinal permeability assessed in vivo using the FITC-dextran test was unaltered. GPR109a deletion did not worsen gastrointestinal permeability. Further, 12.5% resistant starch supplementation, at physiological concentrations, had no effect on intestinal permeability or morphology. These studies indicate that GPR109a does not play a critical role in intestinal homeostasis in a model of type 1 diabetes or in the development of diabetic nephropathy.
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- 2020
9. Delineating a role for the mitochondrial permeability transition pore in diabetic kidney disease by targeting cyclophilin D
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Maryann Arnstein, Melinda T. Coughlan, Cesare Granata, Runa S.J. Lindblom, Josephine M. Forbes, Mark E. Cooper, Darren C. Henstridge, Matthew Snelson, Vicki Thallas-Bonke, Tuong-Vi Nguyen, and Gavin C Higgins
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medicine.medical_specialty ,Mitochondrion ,Kidney ,Mitochondrial Membrane Transport Proteins ,Diabetes Mellitus, Experimental ,Diabetic nephropathy ,chemistry.chemical_compound ,Internal medicine ,medicine ,Albuminuria ,Animals ,Diabetic Nephropathies ,Mice, Knockout ,Alisporivir ,Mitochondrial Permeability Transition Pore ,Chemistry ,MPTP ,Glomerulosclerosis ,Hydrogen Peroxide ,General Medicine ,medicine.disease ,Streptozotocin ,Mitochondria ,Mice, Inbred C57BL ,Proton-Translocating ATPases ,Endocrinology ,Mitochondrial permeability transition pore ,Cyclosporine ,Kidney Diseases ,medicine.symptom ,Cyclophilin D ,medicine.drug - Abstract
Mitochondrial stress has been widely observed in diabetic kidney disease (DKD). Cyclophilin D (CypD) is a functional component of the mitochondrial permeability transition pore (mPTP) which allows the exchange of ions and solutes between the mitochondrial matrix to induce mitochondrial swelling and activation of cell death pathways. CypD has been successfully targeted in other disease contexts to improve mitochondrial function and reduced pathology. Two approaches were used to elucidate the role of CypD and the mPTP in DKD. Firstly, mice with a deletion of the gene encoding CypD (Ppif−/−) were rendered diabetic with streptozotocin (STZ) and followed for 24 weeks. Secondly, Alisporivir, a CypD inhibitor was administered to the db/db mouse model (5 mg/kg/day oral gavage for 16 weeks). Ppif−/− mice were not protected against diabetes-induced albuminuria and had greater glomerulosclerosis than their WT diabetic littermates. Renal hyperfiltration was lower in diabetic Ppif−/− as compared with WT mice. Similarly, Alisporivir did not improve renal function nor pathology in db/db mice as assessed by no change in albuminuria, KIM-1 excretion and glomerulosclerosis. Db/db mice exhibited changes in mitochondrial function, including elevated respiratory control ratio (RCR), reduced mitochondrial H2O2 generation and increased proximal tubular mitochondrial volume, but these were unaffected by Alisporivir treatment. Taken together, these studies indicate that CypD has a complex role in DKD and direct targeting of this component of the mPTP will likely not improve renal outcomes.
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- 2020
10. The Role of AGE-RAGE Signalling as a Modulator of Gut Permeability in Diabetes
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Matthew Snelson, Elisa Lucut, and Melinda T. Coughlan
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Glycation End Products, Advanced ,diabetes ,intestinal permeability ,QH301-705.5 ,Organic Chemistry ,Receptor for Advanced Glycation End Products ,General Medicine ,Catalysis ,Permeability ,Computer Science Applications ,Inorganic Chemistry ,Intestines ,Chemistry ,receptor for advanced glycation endproducts ,Diabetes Mellitus ,Animals ,Humans ,Biology (General) ,Physical and Theoretical Chemistry ,QD1-999 ,Molecular Biology ,advanced glycation endproducts ,Spectroscopy ,Signal Transduction - Abstract
There is increasing evidence for the role of intestinal permeability as a contributing factor in the pathogenesis of diabetes; however, the molecular mechanisms are poorly understood. Advanced glycation endproducts, of both exogenous and endogenous origin, have been shown to play a role in diabetes pathophysiology, in part by their ligation to the receptor for advanced glycation endproducts (RAGE), leading to a proinflammatory signalling cascade. RAGE signalling has been demonstrated to play a role in the development of intestinal inflammation and permeability in Crohn’s disease and ulcerative colitis. In this review, we explore the role of AGE-RAGE signalling and intestinal permeability and explore whether activation of RAGE on the intestinal epithelium may be a downstream event contributing to the pathogenesis of diabetes complications.
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- 2021
11. Reduced Growth, Altered Gut Microbiome and Metabolite Profile, and Increased Chronic Kidney Disease Risk in Young Pigs Consuming a Diet Containing Highly Resistant Protein
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Margaret Murray, Melinda T. Coughlan, Anne Gibbon, Vinod Kumar, Francine Z. Marques, Sophie Selby-Pham, Matthew Snelson, Kirill Tsyganov, Gary Williamson, Trent M. Woodruff, Tong Wu, and Louise E. Bennett
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Nutrition and Dietetics ,Endocrinology, Diabetes and Metabolism ,Food Science - Abstract
High-heat processed foods contain proteins that are partially resistant to enzymatic digestion and pass through to the colon. The fermentation of resistant proteins by gut microbes produces products that may contribute to chronic disease risk. This pilot study examined the effects of a resistant protein diet on growth, fecal microbiome, protein fermentation metabolites, and the biomarkers of health status in pigs as a model of human digestion and metabolism. Weanling pigs were fed with standard or resistant protein diets for 4 weeks. The resistant protein, approximately half as digestible as the standard protein, was designed to enter the colon for microbial fermentation. Fecal and blood samples were collected to assess the microbiome and circulating metabolites and biomarkers. The resistant protein diet group consumed less feed and grew to ~50% of the body mass of the standard diet group. The diets had unique effects on the fecal microbiome, as demonstrated by clustering in the principal coordinate analysis. There were 121 taxa that were significantly different between groups (adjusted-p < 0.05). Compared with control, plasma tri-methylamine-N-oxide, homocysteine, neopterin, and tyrosine were increased and plasma acetic acid was lowered following the resistant protein diet (all p < 0.05). Compared with control, estimated glomerular filtration rate (p < 0.01) and liver function marker aspartate aminotransferase (p < 0.05) were also lower following the resistant protein diet. A resistant protein diet shifted the composition of the fecal microbiome. The microbial fermentation of resistant protein affected the levels of circulating metabolites and the biomarkers of health status toward a profile indicative of increased inflammation and the risk of chronic kidney disease.
- Published
- 2021
12. Targeting Methylglyoxal in Diabetic Kidney Disease Using the Mitochondria-Targeted Compound MitoGamide
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Richard C. Hartley, Michael P. Murphy, Runa S.J. Lindblom, Sih Min Tan, Assam El-Osta, Thomas Krieg, Cesare Granata, Mark Ziemann, Stuart T. Caldwell, Melinda T. Coughlan, Mark E. Cooper, Vicki Thallas-Bonke, Carlos D. Baeza-Garza, Adrienne Laskowski, Matthew Snelson, Lindblom, Runa SJ [0000-0001-6755-8744], Granata, Cesare [0000-0002-3509-6001], Snelson, Matthew [0000-0003-4829-9550], El-Osta, Assam [0000-0001-7968-7375], Baeza-Garza, Carlos D [0000-0002-7423-4948], Krieg, Thomas [0000-0002-5192-580X], Murphy, Michael P [0000-0003-1115-9618], Coughlan, Melinda T [0000-0001-8846-6443], Apollo - University of Cambridge Repository, Lindblom, Runa S. J. [0000-0001-6755-8744], Baeza-Garza, Carlos D. [0000-0002-7423-4948], Murphy, Michael P. [0000-0003-1115-9618], and Coughlan, Melinda T. [0000-0001-8846-6443]
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0301 basic medicine ,Male ,medicine.medical_specialty ,kidney ,030204 cardiovascular system & hematology ,Mitochondrion ,medicine.disease_cause ,Article ,Diabetes Mellitus, Experimental ,Diabetes Complications ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,0302 clinical medicine ,Diabetes mellitus ,Internal medicine ,medicine ,Renal fibrosis ,methylglyoxal ,Animals ,TX341-641 ,Kidney ,dicarbonyl ,Nutrition and Dietetics ,diabetes ,business.industry ,Nutrition. Foods and food supply ,sugar-derived products ,MitoGamide ,Methylglyoxal ,Glomerulosclerosis ,medicine.disease ,Pyruvaldehyde ,Mitochondria ,Mice, Inbred C57BL ,Disease Models, Animal ,030104 developmental biology ,Endocrinology ,medicine.anatomical_structure ,chemistry ,Benzamides ,Albuminuria ,Kidney Diseases ,medicine.symptom ,business ,Oxidative stress ,Food Science - Abstract
Diabetic kidney disease (DKD) remains the number one cause of end-stage renal disease in the western world. In experimental diabetes, mitochondrial dysfunction in the kidney precedes the development of DKD. Reactive 1,2-dicarbonyl compounds, such as methylglyoxal, are generated from sugars both endogenously during diabetes and exogenously during food processing. Methylglyoxal is thought to impair the mitochondrial function and may contribute to the pathogenesis of DKD. Here, we sought to target methylglyoxal within the mitochondria using MitoGamide, a mitochondria-targeted dicarbonyl scavenger, in an experimental model of diabetes. Male 6-week-old heterozygous Akita mice (C57BL/6-Ins2-Akita/J) or wildtype littermates were randomized to receive MitoGamide (10 mg/kg/day) or a vehicle by oral gavage for 16 weeks. MitoGamide did not alter the blood glucose control or body composition. Akita mice exhibited hallmarks of DKD including albuminuria, hyperfiltration, glomerulosclerosis, and renal fibrosis, however, after 16 weeks of treatment, MitoGamide did not substantially improve the renal phenotype. Complex-I-linked mitochondrial respiration was increased in the kidney of Akita mice which was unaffected by MitoGamide. Exploratory studies using transcriptomics identified that MitoGamide induced changes to olfactory signaling, immune system, respiratory electron transport, and post-translational protein modification pathways. These findings indicate that targeting methylglyoxal within the mitochondria using MitoGamide is not a valid therapeutic approach for DKD and that other mitochondrial targets or processes upstream should be the focus of therapy.
- Published
- 2021
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13. Resistant Starch as a Dietary Intervention to Limit the Progression of Diabetic Kidney Disease
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Anna M. Drake, Melinda T. Coughlan, Claus T. Christophersen, and Matthew Snelson
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Dietary Fiber ,Nutrition and Dietetics ,Diabetes Mellitus ,Animals ,Humans ,Resistant Starch ,Starch ,Diabetic Nephropathies ,Fatty Acids, Volatile ,Food Science - Abstract
Diabetes is the leading cause of kidney disease, and as the number of individuals with diabetes increases there is a concomitant increase in the prevalence of diabetic kidney disease (DKD). Diabetes contributes to the development of DKD through a number of pathways, including inflammation, oxidative stress, and the gut-kidney axis, which may be amenable to dietary therapy. Resistant starch (RS) is a dietary fibre that alters the gut microbial consortium, leading to an increase in the microbial production of short chain fatty acids. Evidence from animal and human studies indicate that short chain fatty acids are able to attenuate inflammatory and oxidative stress pathways, which may mitigate the progression of DKD. In this review, we evaluate and summarise the evidence from both preclinical models of DKD and clinical trials that have utilised RS as a dietary therapy to limit the progression of DKD.
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- 2022
14. Renal ACE2 (Angiotensin-Converting Enzyme 2) Expression Is Modulated by Dietary Fiber Intake, Gut Microbiota, and Their Metabolites
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Melinda T. Coughlan, Waled A. Shihata, Louise M Burrell, Charles R. Mackay, Francine Z. Marques, Rikeish R. Muralitharan, Michael E Nakai, Matthew Snelson, David M. Kaye, Hamdi A. Jama, Chad Johnson, and Evany Dinakis
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Dietary Fiber ,medicine.medical_specialty ,biology ,business.industry ,Metabolite ,Gut flora ,Kidney ,biology.organism_classification ,medicine.disease ,Gastrointestinal Microbiome ,Mice ,chemistry.chemical_compound ,Endocrinology ,chemistry ,Fibrosis ,Internal medicine ,Angiotensin-converting enzyme 2 ,Internal Medicine ,medicine ,Animals ,Dietary fiber ,Angiotensin-Converting Enzyme 2 ,business - Published
- 2021
15. SOD2 in Skeletal Muscle: New Insights from an Inducible Deletion Model
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Peter J. Meikle, Anna C. Calkin, Brian G. Drew, Christine Yang, Tim Sikora, J. B. de Haan, Melinda T. Coughlan, Simon T. Bond, Arpeeta Sharma, Y. Tan, S. Walker, Aowen Zhuang, and Yingying Liu
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chemistry.chemical_classification ,Reactive oxygen species ,biology ,Chemistry ,SOD1 ,SOD2 ,Skeletal muscle ,Lipid metabolism ,Mitochondrion ,medicine.disease_cause ,Cell biology ,Superoxide dismutase ,medicine.anatomical_structure ,biology.protein ,medicine ,Oxidative stress - Abstract
Metabolic conditions such as obesity, insulin resistance and glucose intolerance are frequently associated with impairments in skeletal muscle function and metabolism. This is often linked to dysregulation of homeostatic pathways including an increase in reactive oxygen species (ROS) and oxidative stress. One of the main sites of ROS production is the mitochondria, where the flux of substrates through the electron transport chain (ETC) can result in the generation of oxygen free radicals. Fortunately, several mechanisms exist to buffer bursts of intracellular ROS and peroxide production, including the enzymes Catalase, Glutathione Peroxidase and Superoxide Dismutase (SOD). Of the latter there are two intracellular isoforms; SOD1 which is mostly cytoplasmic, and SOD2 which is found exclusively in the mitochondria. Developmental and chronic loss of these enzymes has been linked to disease in several studies, however the temporal effects of these disturbances remain largely unexplored. Here, we induced a post-developmental (8-week old mice) deletion of SOD2 in skeletal muscle (SOD2-iMKO) and demonstrate that 16 weeks of SOD2 deletion leads to no major impairment in whole body metabolism, despite these mice displaying alterations in aspects of mitochondrial abundance and voluntary ambulatory movement. Furthermore, we demonstrated that SOD2 deletion impacts on specific aspects of muscle lipid metabolism, including the abundance of phospholipids and phosphatidic acid (PA), the latter being a key intermediate in several cellular signaling pathways. Thus, our findings suggest that post-developmental deletion of SOD2 induces a more subtle phenotype than previous embryonic models have shown, allowing us to highlight a previously unrecognized link between SOD2, mitochondrial function and bioactive lipid species including PA.
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- 2021
16. Complement C5a Induces Renal Injury in Diabetic Kidney Disease by Disrupting Mitochondrial Metabolic Agility
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Alison Skene, Trent M. Woodruff, Assam El-Osta, Mark E. Cooper, Kevin Huynh, Adrienne Laskowski, Darren C. Henstridge, Scott T. Baker, Vicki Thallas-Bonke, Matthew Snelson, Renata Libianto, Rick A. Wetsel, Melinda T. Coughlan, Peter J. Meikle, Josephine M. Forbes, Richard J MacIsaac, Sih Min Tan, Mark Ziemann, Scott Wilson, Tuong-Vi Nguyen, Michele V Clarke, Elif I Ekinci, David A. Power, and Vinod Kumar
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Male ,0301 basic medicine ,Endocrinology, Diabetes and Metabolism ,Complement C5a ,Mice, Transgenic ,030209 endocrinology & metabolism ,Inflammation ,Pharmacology ,Mitochondrion ,Kidney ,Diabetes Mellitus, Experimental ,Rats, Sprague-Dawley ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Downregulation and upregulation ,Diabetes mellitus ,Internal Medicine ,medicine ,Cardiolipin ,Animals ,Humans ,Diabetic Nephropathies ,Respiratory function ,Receptor, Anaphylatoxin C5a ,Cells, Cultured ,business.industry ,medicine.disease ,Fibrosis ,Mitochondria ,Rats ,Mice, Inbred C57BL ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,medicine.symptom ,Energy Metabolism ,business ,Signal Transduction ,Kidney disease - Abstract
The sequelae of diabetes include microvascular complications such as diabetic kidney disease (DKD), which involves glucose-mediated renal injury associated with a disruption in mitochondrial metabolic agility, inflammation, and fibrosis. We explored the role of the innate immune complement component C5a, a potent mediator of inflammation, in the pathogenesis of DKD in clinical and experimental diabetes. Marked systemic elevation in C5a activity was demonstrated in patients with diabetes; conventional renoprotective agents did not therapeutically target this elevation. C5a and its receptor (C5aR1) were upregulated early in the disease process and prior to manifest kidney injury in several diverse rodent models of diabetes. Genetic deletion of C5aR1 in mice conferred protection against diabetes-induced renal injury. Transcriptomic profiling of kidney revealed diabetes-induced downregulation of pathways involved in mitochondrial fatty acid metabolism. Interrogation of the lipidomics signature revealed abnormal cardiolipin remodeling in diabetic kidneys, a cardinal sign of disrupted mitochondrial architecture and bioenergetics. In vivo delivery of an orally active inhibitor of C5aR1 (PMX53) reversed the phenotypic changes and normalized the renal mitochondrial fatty acid profile, cardiolipin remodeling, and citric acid cycle intermediates. In vitro exposure of human renal proximal tubular epithelial cells to C5a led to altered mitochondrial respiratory function and reactive oxygen species generation. These experiments provide evidence for a pivotal role of the C5a/C5aR1 axis in propagating renal injury in the development of DKD by disrupting mitochondrial agility, thereby establishing a new immunometabolic signaling pathway in DKD.
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- 2019
17. Processed foods drive intestinal barrier permeability and microvascular diseases
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Leigh Donnellan, Mark E. Cooper, Sih Min Tan, Brooke E. Harcourt, Mark Ziemann, Nicole J. Kellow, Josephine M. Forbes, Karly C. Sourris, Melinda T. Coughlan, David Steer, Rachel E. Clarke, Charles R. Mackay, Assam El-Osta, Sally A. Penfold, Tuong-Vi Nguyen, Matthew Snelson, Michael J. Davies, Cassandra de Pasquale, Vicki Thallas-Bonke, Runa S.J. Lindblom, Permal Deo, Trent M. Woodruff, Snelson, Matthew, Min Tan, Sih, Clarke, Rachel E, de Pasquale, Cassandra, Donnellan, Leigh, Deo, Permal, and Coughlan, Melinda T
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Male ,food.ingredient ,processed foods ,Diseases and Disorders ,030209 endocrinology & metabolism ,Inflammation ,Pharmacology ,Permeability ,Proinflammatory cytokine ,Mice ,03 medical and health sciences ,0302 clinical medicine ,food ,Glycation ,Diabetes mellitus ,medicine ,Animals ,Humans ,Health and Medicine ,Renal Insufficiency, Chronic ,Resistant starch ,Research Articles ,030304 developmental biology ,0303 health sciences ,Multidisciplinary ,Innate immune system ,business.industry ,fungi ,food and beverages ,SciAdv r-articles ,biochemical phenomena, metabolism, and nutrition ,medicine.disease ,Diet ,Complement system ,Food ,Female ,medicine.symptom ,business ,chronic kidney disease ,Research Article ,Kidney disease - Abstract
This study shows how highly processed foods can cause innate immune inflammation that promotes chronic microvascular disease., Intake of processed foods has increased markedly over the past decades, coinciding with increased microvascular diseases such as chronic kidney disease (CKD) and diabetes. Here, we show in rodent models that long-term consumption of a processed diet drives intestinal barrier permeability and an increased risk of CKD. Inhibition of the advanced glycation pathway, which generates Maillard reaction products within foods upon thermal processing, reversed kidney injury. Consequently, a processed diet leads to innate immune complement activation and local kidney inflammation and injury via the potent proinflammatory effector molecule complement 5a (C5a). In a mouse model of diabetes, a high resistant starch fiber diet maintained gut barrier integrity and decreased severity of kidney injury via suppression of complement. These results demonstrate mechanisms by which processed foods cause inflammation that leads to chronic disease.
- Published
- 2021
18. Training-induced bioenergetic improvement in human skeletal muscle is associated with non-stoichiometric changes in the mitochondrial proteome without reorganization of respiratory chain content
- Author
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Ann E. Frazier, David A. Stroud, Nicholas A. Jamnick, David R. Thorburn, Adrienne Laskowski, Melinda T. Coughlan, Javier Botella, Nikeisha J. Caruana, Boris Reljic, Kevin Huynh, Tegan Stait, David Bishop, H. Janssen, Cesare Granata, Natalie A. Mellett, Peter J. Meikle, and Jujiao Kuang
- Subjects
medicine.anatomical_structure ,Bioenergetics ,In silico ,Respiratory chain ,medicine ,Skeletal muscle ,Electron flow ,Oxidative phosphorylation ,Biology ,Mitochondrial proteome ,Cell biology - Abstract
SUMMARYMitochondrial defects are implicated in multiple diseases and aging. Exercise training is an accessible and inexpensive therapeutic intervention improving mitochondrial bioenergetics and quality of life. By combining a multi-omics approach with biochemical and in silico normalization, we removed the bias arising from the training-induced increase in human skeletal muscle mitochondrial content to unearth an intricate and previously undemonstrated network of differentially prioritized mitochondrial adaptations. We show that changes in hundreds of transcripts, proteins, and lipids are not stoichiometrically linked to the increase in mitochondrial content. We demonstrate that enhancing electron flow to oxidative phosphorylation (OXPHOS) is more important to improve ATP generation than increasing the abundance of the OXPHOS machinery, and that training-induced supercomplex formation does not confer enhancements in mitochondrial bioenergetics. Our study provides a new analytical approach allowing unbiased and in-depth investigations of training-induced mitochondrial adaptations, challenging our current understanding and calling for careful reinterpretation of previous findings.
- Published
- 2021
19. SOD2 in skeletal muscle: New insights from an inducible deletion model
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Anna C. Calkin, Simon T. Bond, Takahiko Shimizu, Judy B. de Haan, Adrienne Laskowski, Peter J. Meikle, Aowen Zhuang, Yanie Tan, Tim Sikora, Yingying Liu, Shannen Walker, Brian G. Drew, Arpeeta Sharma, Melinda T. Coughlan, and Christine Yang
- Subjects
Medicine (General) ,QH301-705.5 ,Clinical Biochemistry ,SOD2 ,Skeletal muscle ,Mitochondrion ,medicine.disease_cause ,Biochemistry ,Superoxide dismutase ,Mice ,R5-920 ,medicine ,Animals ,Biology (General) ,Muscle, Skeletal ,Uncategorized ,chemistry.chemical_classification ,Reactive oxygen species ,biology ,Superoxide Dismutase ,Glutathione peroxidase ,Organic Chemistry ,Superoxide ,Lipid metabolism ,ROS ,Cell biology ,Mitochondria ,Oxidative Stress ,medicine.anatomical_structure ,chemistry ,biology.protein ,Reactive Oxygen Species ,Oxidative stress ,Research Paper - Abstract
Metabolic conditions such as obesity, insulin resistance and glucose intolerance are frequently associated with impairments in skeletal muscle function and metabolism. This is often linked to dysregulation of homeostatic pathways including an increase in reactive oxygen species (ROS) and oxidative stress. One of the main sites of ROS production is the mitochondria, where the flux of substrates through the electron transport chain (ETC) can result in the generation of oxygen free radicals. Fortunately, several mechanisms exist to buffer bursts of intracellular ROS and peroxide production, including the enzymes Catalase, Glutathione Peroxidase and Superoxide Dismutase (SOD). Of the latter, there are two intracellular isoforms; SOD1 which is mostly cytoplasmic, and SOD2 which is found exclusively in the mitochondria. Developmental and chronic loss of these enzymes has been linked to disease in several studies, however the temporal effects of these disturbances remain largely unexplored. Here, we induced a post-developmental (8-week old mice) deletion of SOD2 in skeletal muscle (SOD2-iMKO) and demonstrate that 16 weeks of SOD2 deletion leads to no major impairment in whole body metabolism, despite these mice displaying alterations in aspects of mitochondrial abundance and voluntary ambulatory movement. This is likely partly explained by the suggestive data that a compensatory response may exist from other redox enzymes, including catalase and glutathione peroxidases. Nevertheless, we demonstrated that inducible SOD2 deletion impacts on specific aspects of muscle lipid metabolism, including the abundance of phospholipids and phosphatidic acid (PA), the latter being a key intermediate in several cellular signaling pathways. Thus, our findings suggest that post-developmental deletion of SOD2 induces a more subtle phenotype than previous embryonic models have shown, allowing us to highlight a previously unrecognized link between SOD2, mitochondrial function and bioactive lipid species including PA.
- Published
- 2021
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20. Thermally processed diet-induced albuminuria, Enterobacteriaceae expansion and cecal metabolome alterations are attenuated by resistant starch in diabetes
- Author
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Vicki Thallas-Bonke, Karly C. Sourris, Sih Tan, Melinda T. Coughlan, Runa S.J. Lindblom, Mark E. Cooper, and Matthew Snelson
- Subjects
food.ingredient ,biology ,Chemistry ,medicine.disease ,biology.organism_classification ,Enterobacteriaceae ,Microbiology ,food ,Diabetes mellitus ,medicine ,Albuminuria ,Metabolome ,Resistant starch ,medicine.symptom - Published
- 2020
21. 485-P: T Lymphocytes Infiltration in Kidneys of People with Type 2 Diabetes
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Melinda T. Coughlan, Niloufar Torkamani, Evelyn C. Marin, Richard J MacIsaac, Elif I Ekinci, Laura K. Mackay, David A. Power, Sof Andrikopoulos, and Lingyun Kong
- Subjects
medicine.medical_specialty ,medicine.diagnostic_test ,business.industry ,Endocrinology, Diabetes and Metabolism ,Renal function ,Disease ,Type 2 diabetes ,medicine.disease ,Gastroenterology ,Internal medicine ,Diabetes mellitus ,Internal Medicine ,medicine ,Albuminuria ,Renal biopsy ,medicine.symptom ,Complication ,business ,Kidney disease - Abstract
Diabetic kidney disease (DKD) is a highly prevalent complication of diabetes and is the leading cause of ESKD. The role of T lymphocytes in DKD remains unclear. We aimed to determine the distribution of T lymphocytes in renal biopsies in people with T2DM with varying degrees of kidney function and albuminuria. Fresh renal biopsy samples were provided by the Victorian Cancer Biobank at the time of surgery from individuals undergoing partial/complete nephrectomy or from people with diabetes who had a renal biopsy for clinical indications at Austin Health. Participants were divided into the following groups: Diabetic kidney disease group (DKD was diagnosed by clinical and histologic characteristics), nondiabetic and intact renal function (Control) (eGFR > 60ml/min/m2, no albuminuria) and nondiabetic kidney disease (NDKD) (eGFR < 60ml/min/m2, no history of diabetes). DKD group was further stratified into normo-, micro-, or macroalbuminuria groups. Sections were immunostained with CD3 antibody. Results showed higher level of creatinine, systolic blood pressure, and reduced level of eGFR, poor glycemic control in people with DKD and macroalbuminuria as compared with people without diabetes. T lymphocytes numbers were significantly increased in the interstitial compartment of people with T2DM and macroalbuminuria compared to those without diabetes, suggesting the immunopathologic role of T lymphocytes in the development of DKD. Disclosure L. Kong: None. S. Andrikopoulos: None. R. MacIsaac: None. N. Torkamani: None. M. Coughlan: None. D. Power: None. E.C. Marin: None. L.K. Mackay: None. E.I. Ekinci: None.
- Published
- 2020
22. Methods in renal research: Measurement of autophagic flux in the renal cortex ex vivo
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Georg Ramm, Gavin C Higgins, Tuong-Vi Nguyen, and Melinda T. Coughlan
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Male ,0301 basic medicine ,Kidney cortex ,Kidney Cortex ,Time Factors ,Renal cortex ,Autophagy-Related Proteins ,In Vitro Techniques ,03 medical and health sciences ,Microscopy, Electron, Transmission ,Autophagy ,Animals ,Medicine ,Sirolimus ,Kidney ,business.industry ,Autophagosomes ,General Medicine ,Cell biology ,Mice, Inbred C57BL ,030104 developmental biology ,medicine.anatomical_structure ,Gene Expression Regulation ,Nephrology ,Macrolides ,business ,Flux (metabolism) ,Ex vivo ,Signal Transduction ,medicine.drug - Abstract
The role of autophagy in the kidney and many nephrological diseases has gained prominence in recent years. Much of this research has been focused on markers of autophagy that are static and reveal little about the state of this dynamic pathway. Other mechanistic investigations are limited to in vitro studies, that often provide circumstantial evidence of autophagic flux. Here we describe a method for measuring autophagic flux ex vivo that allows more direct observations to be made in situ regarding the state of autophagic flux within the renal cortex of a single animal.
- Published
- 2018
23. RAGE Deletion Confers Renoprotection by Reducing Responsiveness to Transforming Growth Factor-β and Increasing Resistance to Apoptosis
- Author
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Shinji Hagiwara, Raelene Pickering, Karly C. Sourris, Josephine M. Forbes, Gavin C Higgins, Muthukumar Mohan, Phillip Kantharidis, Bo Wang, Assam El-Osta, Wu Tieqiao, Brooke E. Harcourt, Mark Ziemann, Mark E. Cooper, Sally A. Penfold, Melinda T. Coughlan, Eoin P. Brennan, Aaron McClelland, and Merlin C. Thomas
- Subjects
Collagen Type IV ,0301 basic medicine ,Kidney Cortex ,endocrine system diseases ,Cell Survival ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Receptor for Advanced Glycation End Products ,Apoptosis ,Kidney ,Collagen Type I ,Diabetes Mellitus, Experimental ,RAGE (receptor) ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Transforming Growth Factor beta ,Internal Medicine ,medicine ,Animals ,Diabetic Nephropathies ,cardiovascular diseases ,Cells, Cultured ,Chemokine CCL2 ,Cell Proliferation ,Mice, Knockout ,Regulation of gene expression ,biology ,Growth factor ,nutritional and metabolic diseases ,Kidney metabolism ,Transforming growth factor beta ,Fibronectins ,030104 developmental biology ,Gene Expression Regulation ,Mesangial Cells ,Knockout mouse ,cardiovascular system ,biology.protein ,Cancer research ,Signal transduction ,human activities ,Signal Transduction ,030215 immunology ,Transforming growth factor - Abstract
Signaling via the receptor of advanced glycation end products (RAGE)—though complex and not fully elucidated in the setting of diabetes—is considered a key injurious pathway in the development of diabetic nephropathy (DN). We report here that RAGE deletion resulted in increased expression of fibrotic markers (collagen I and IV, fibronectin) and the inflammatory marker MCP-1 in primary mouse mesangial cells (MCs) and in kidney cortex. RNA sequencing analysis in MCs from RAGE−/− and wild-type mice confirmed these observations. Nevertheless, despite these gene expression changes, decreased responsiveness to transforming growth factor-β was identified in RAGE−/− mice. Furthermore, RAGE deletion conferred a more proliferative phenotype in MCs and reduced susceptibility to staurosporine-induced apoptosis. RAGE restoration experiments in RAGE−/− MCs largely reversed these gene expression changes, resulting in reduced expression of fibrotic and inflammatory markers. This study highlights that protection against DN in RAGE knockout mice is likely to be due in part to the decreased responsiveness to growth factor stimulation and an antiapoptotic phenotype in MCs. Furthermore, it extends our understanding of the role of RAGE in the progression of DN, as RAGE seems to play a key role in modulating the sensitivity of the kidney to injurious stimuli such as prosclerotic cytokines.
- Published
- 2018
24. Thermally Processed Diet-Induced Albuminuria, Complement Activation and Intestinal Permeability Are Attenuated by Resistant Starch in Experimental Diabetes
- Author
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Sih Min Tan, Vicki Thallas-Bonke, Josephine M. Forbes, Assam El-Osta, Mark Ziemann, Melinda T. Coughlan, Mark E. Cooper, Matthew Snelson, and Karly C. Sourris
- Subjects
medicine.medical_specialty ,Nutrition and Dietetics ,Intestinal permeability ,food.ingredient ,Chemistry ,Medicine (miscellaneous) ,medicine.disease ,Complement system ,Endocrinology ,food ,Food Science and Nutrition ,Internal medicine ,Albuminuria ,medicine ,medicine.symptom ,Resistant starch ,Food Science ,Experimental diabetes - Abstract
OBJECTIVES: The primary objective of this study was to ascertain whether thermally processed diets influence albuminuria and intestinal permeability via alterations in the complement cascade. A secondary objective was to see whether these pathological alterations could be ameliorated by a gut-targeted dietary intervention, resistant starch. METHODS: Six-week-old Sprague Dawley rats were randomised to receive a control (CON; AIN93G), thermally processed diet (TPD) (AIN93G baked at 160°C for 1h) or TPD with daily gavage of either 10 mg/kg/d alagebrium chloride (ALA), an inhibitor of advanced glycation end products or daily gavage of 2mg/kg/d PMX-53, a C5a receptor inhibitor for 24 weeks. Six-week-old diabetic mice (db/db) received the CON diet or TPD with or without 12.5% resistant starch (RS) for 10 weeks. Albumin, MCP-1 and C5a were measured by ELISA. Endotoxin was measured using a limulus amoebocyte lysate kit. Intestinal permeability was assessed in vivo by the clearance of FITC-labelled dextran. Transcriptomic profiling of renal cortex was determined by RNA-Sequencing. RESULTS: The TPD increased albuminuria, plasma endotoxin and MCP-1 which were ameliorated with ALA or PMX-53. TPD increased urinary C5a, which was decreased with ALA. In db/db mice, RS supplementation of the TPD reduced albuminuria and intestinal permeability. Gene set enrichment analysis showed an upregulation in the complement cascade in TPD db/db mice, which was normalized by RS. Similarly, RS supplementation reduced urinary C5a in TPD-fed db/db mice. CONCLUSIONS: These results demonstrate that thermally processed diets lead to worsening albuminuria via activation of the complement cascade. These results also indicate that resistant starch supplementation may ameliorate some of the negative effects observed with excessive intake of thermally processed. FUNDING SOURCES: This study was funded by the National Health and Medical Research Council of Australia (NHMRC) and the Australian and New Zealand Society of Nephrology (ANZSN).
- Published
- 2021
25. Gut microbiome, prebiotics, intestinal permeability and diabetes complications
- Author
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Elif I Ekinci, Cassandra de Pasquale, Matthew Snelson, and Melinda T. Coughlan
- Subjects
0301 basic medicine ,medicine.medical_specialty ,food.ingredient ,Endocrinology, Diabetes and Metabolism ,030209 endocrinology & metabolism ,Butyrate ,Type 2 diabetes ,Gut flora ,Permeability ,Diabetes Complications ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,Insulin resistance ,food ,Internal medicine ,Diabetes mellitus ,medicine ,Humans ,Resistant starch ,Intestinal permeability ,biology ,business.industry ,medicine.disease ,Glucagon-like peptide-2 ,biology.organism_classification ,Gastrointestinal Microbiome ,Prebiotics ,030104 developmental biology ,Diabetes Mellitus, Type 2 ,business - Abstract
Diabetes is a metabolic condition. The composition of the gut microbiota is altered in diabetes with reduced levels of short chain fatty acids (SCFA) producers, notably butyrate. Butyrate is associated with a number of beneficial effects including promoting the integrity of the gastrointestinal barrier. Diabetes may lead to an increase in the permeability of the gut barrier, which is thought to contribute to systemic inflammation and worsen the microvascular complications of diabetes. Prebiotics, non-digestible carbohydrates, are fermented by the colonic microbiota leading to the production of a range of metabolites including SCFAs. Thus, prebiotics represent a dietary approach to increase levels of microbially produced SCFAs and improve intestinal permeability in diabetes. Whether prebiotics can lead to a reduction in the risk of developing diabetes complications in individuals with type 2 diabetes needs to be explored.
- Published
- 2021
26. Association between habitual dietary and lifestyle behaviours and skin autofluorescence (SAF), a marker of tissue accumulation of advanced glycation endproducts (AGEs), in healthy adults
- Author
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Christopher M. Reid, Nicole J. Kellow, and Melinda T. Coughlan
- Subjects
Adult ,Glycation End Products, Advanced ,Male ,0301 basic medicine ,Pathology ,medicine.medical_specialty ,Meat ,Waist ,Adolescent ,Adult population ,Medicine (miscellaneous) ,Physiology ,030209 endocrinology & metabolism ,Body weight ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Cigarette smoking ,Humans ,Medicine ,Prospective Studies ,Life Style ,Pathological ,Aged ,Skin ,Aged, 80 and over ,030109 nutrition & dietetics ,Nutrition and Dietetics ,business.industry ,Smoking ,fungi ,Age Factors ,Skin autofluorescence ,Middle Aged ,Advanced Glycation Endproducts ,Diet ,Meat Products ,Blood pressure ,Female ,business - Abstract
Advanced glycation endproducts (AGEs) are produced endogenously and also enter the body during the consumption of AGEs present in heat-processed food. It is unknown whether AGEs of dietary origin accumulate within the body of healthy individuals. AGEs can deposit within skin tissue long-term by crosslinking extracellular matrix proteins. The fluorescent nature of many AGEs enables their detection within the skin by non-invasively measuring skin autofluorescence (SAF). This study aimed to identify habitual dietary and lifestyle behaviours cross-sectionally associated with SAF in an adult population sample. 251 Healthy adult volunteers completed validated food frequency and physical activity questionnaires. Waist circumference, BMI, blood pressure and blood glucose was also measured. SAF was measured using an AGE Reader. Significant positive correlations were found between SAF and chronological age (r = 0.63, P
- Published
- 2017
27. The Devil's in the Detail: The Importance of Specific, Descriptive Language for Reproducibility in Nutrition Science
- Author
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Matthew Snelson and Melinda T. Coughlan
- Subjects
Reproducibility ,Medical education ,Nutrition and Dietetics ,Nutritional Sciences ,business.industry ,Flour ,Reproducibility of Results ,Resistant Starch ,Medicine (miscellaneous) ,Descriptive language ,Nutrition science ,Diabetes Mellitus, Type 2 ,Nephrology ,Humans ,Medicine ,Diabetic Nephropathies ,business ,Language - Published
- 2020
28. Globally elevating the AGE clearance receptor, OST48, does not protect against the development of diabetic kidney disease, despite improving insulin secretion
- Author
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Brooke E. Harcourt, Josephine M. Forbes, Felicia Y. T. Yap, Benjamin L. Schulz, Sally A. Penfold, Karly C. Sourris, Melinda T. Coughlan, Vicki Thallas-Bonke, Aowen Zhuang, Sherman S. Leung, Domenica A. McCarthy, and Christopher Leung
- Subjects
0303 health sciences ,Kidney ,medicine.medical_specialty ,business.industry ,Insulin ,medicine.medical_treatment ,Glomerulosclerosis ,Renal function ,030209 endocrinology & metabolism ,medicine.disease ,03 medical and health sciences ,0302 clinical medicine ,medicine.anatomical_structure ,Endocrinology ,Renal physiology ,Internal medicine ,Diabetes mellitus ,medicine ,Tubulointerstitial fibrosis ,Albuminuria ,medicine.symptom ,business ,030304 developmental biology - Abstract
The accumulation of advanced glycation end products (AGEs) have been implicated in the development and progression of diabetic kidney disease (DKD). There has been interest in investigating the potential of AGE clearance receptors, such as oligosaccharyltransferase-48kDa subunit (OST48) to prevent the detrimental effects of excess AGE accumulation seen in the diabetic kidney. Here the objective of the study was to increase the expression of OST48 to examine if this slowed the development of DKD by facilitating the clearance of AGEs. Groups of 8-week-old heterozygous knock-in male mice (n=9-12/group) over-expressing the gene encoding for OST48, dolichyl-diphosphooligosaccharide-protein glycosyltransferase (DDOST+/-) and litter mate controls were randomised to either (i) no diabetes or (ii) diabetes induced via multiple low-dose streptozotocin and followed for 24 weeks. By the study end, global over expression of OST48 increased glomerular OST48. This facilitated greater renal excretion of AGEs but did not affect circulating or renal AGE concentrations. Diabetes resulted in kidney damage including lower glomerular filtration rate, albuminuria, glomerulosclerosis and tubulointerstitial fibrosis. In diabetic mice, tubulointerstitial fibrosis was further exacerbated by global increases in OST48. There was significantly insulin effectiveness, increased acute insulin secretion, fasting insulin concentrations and AUCinsulin observed during glucose tolerance testing in diabetic mice with global elevations in OST48 when compared to diabetic wild-type littermates. Overall, this study suggested that despite facilitating urinaryrenal AGE clearance, there were no benefits observed on kidney functional and structural parameters in diabetes afforded by globally increasing OST48 expression. However, the improvements in insulin secretion seen in diabetic mice with global over-expression of OST48 and their dissociation from effects on kidney function warrant future investigation.
- Published
- 2019
29. Nuclear Expression and DNA Binding Capacity of Receptor for Advanced Glycation End Products in Renal Tissue
- Author
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Melinda T. Coughlan, Brooke E. Harcourt, Hiroshi Yamamoto, Phillip Kantharidis, Josephine M. Forbes, David W. Johnson, Mark E. Cooper, Sally A. Penfold, Aaron McClelland, Hideto Yonekura, David A. Vesey, Yasuhiko Yamamoto, and Amelia K. Fotheringham
- Subjects
Gene isoform ,endocrine system diseases ,Chemistry ,Cell ,nutritional and metabolic diseases ,Promoter ,Cell biology ,medicine.anatomical_structure ,Glycation ,cardiovascular system ,medicine ,Electrophoretic mobility shift assay ,cardiovascular diseases ,Signal transduction ,Receptor ,human activities ,Gene - Abstract
TheAGERgene encodes for a number of RAGE isoforms, with the membrane bound signal transduction and “decoy” circulating soluble RAGE being the best characterised. Here we demonstrate a novel nuclear isoform of RAGE in mice and human kidney cortex which by cell and size fractionation we determined to be approximately 37kda. This nuclear RAGE isoform is functional and binds to DNA sequences within the upstream 5’ promoter region of its own gene,AGER. This binding was shown to be abrogated by mutating the DNA consensus binding sequences during electromobility shift assay (EMSA) and was independent of NF-□B or AP-1 binding. Cotransfection of expression constructs encoding various RAGE isoforms along withAGERgene promoter reporter-plasmids identified that the most likely source of the nuclear isoform of RAGE was a cleavage product of the nt-RAGE isoform. In obese mice with impaired kidney function, there was increased binding of nuclear RAGE within the A. Region ofagergene promoter with corresponding increases in membrane bound RAGE in renal cells. These findings were reproducedin vitrousing proximal tubule cells. Hence, we postulate that RAGE expression is in part, self-regulated by the binding of a nuclear RAGE isoform to the promoter of theAGERgene (encoding RAGE) in the kidney. We also suggest that this RAGE self-regulation is altered under pathological conditions and this may have implications for chronic kidney disease.
- Published
- 2019
30. Long Term High Protein Diet Feeding Alters the Microbiome and Increases Intestinal Permeability, Systemic Inflammation and Kidney Injury in Mice
- Author
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Josephine M. Forbes, Sih Min Tan, Sally A. Penfold, Rachel E. Clarke, Tuong-Vi Nguyen, Matthew Snelson, and Melinda T. Coughlan
- Subjects
Male ,0301 basic medicine ,medicine.medical_specialty ,Gene Expression ,High-protein diet ,Gut flora ,Kidney ,medicine.disease_cause ,Systemic inflammation ,Occludin ,Permeability ,03 medical and health sciences ,Internal medicine ,medicine ,Albuminuria ,Animals ,Blood urea nitrogen ,Chemokine CCL2 ,Inflammation ,030109 nutrition & dietetics ,Intestinal permeability ,biology ,Chemistry ,Body Weight ,Acute Kidney Injury ,biology.organism_classification ,medicine.disease ,Fibrosis ,Gastrointestinal Microbiome ,Intestines ,Mice, Inbred C57BL ,030104 developmental biology ,Endocrinology ,Urea cycle ,Diet, High-Protein ,medicine.symptom ,Food Science ,Biotechnology - Abstract
Scope: This study evaluates the effects of a chronic high protein diet (HPD) on kidney injury, intestinal permeability and gut microbiota perturbations in a mouse model. Method and results: Mice are fed a diet containing either 20% or 52% energy from protein for 24 weeks; protein displaced an equivalent amount of wheat starch. The HPD does not alter glycemic control or body weight. The HPD induces kidney injury as evidenced by increase in albuminuria, urinary kidney injury molecule-1, blood urea nitrogen, urinary isoprostanes and renal cortical NF-κB p65 gene expression. HPD decreases intestinal occludin gene expression, increases plasma endotoxin and plasma monocyte chemoattractant protein-1, indicating intestinal leakiness and systemic inflammation. Cecal microbial analysis reveals that HPD feeding does not alter alpha diversity; however, it does alter beta diversity, indicating an altered microbial community structure with HPD feeding. Predicted metagenome pathway analysis demonstrates a reduction in branched-chain amino acid synthesis and an increase of the urea cycle with consumption of a HPD. Conclusion: These results demonstrate that long term HPD consumption in mice causes albuminuria, systemic inflammation, increase in gastrointestinal permeability and is associated with gut microbiome remodeling with an increase in the urea cycle pathway, which may contribute to renal injury.
- Published
- 2021
31. Characterising the Myocardial Mitochondria Phenotype in a Murine Model of Diabetic Cardiomyopathy
- Author
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Melinda T. Coughlan, M. De Blasio, M. Deo, A. Parker, Helen Kiriazis, Rebecca H. Ritchie, Simon Crawford, Mitchel Tate, Darnel Prakoso, Andrew M Willis, D. Donner, David M Nash, and Cesare Granata
- Subjects
Pulmonary and Respiratory Medicine ,Pathology ,medicine.medical_specialty ,business.industry ,Murine model ,Diabetic cardiomyopathy ,Medicine ,Myocardial Mitochondria ,Cardiology and Cardiovascular Medicine ,business ,medicine.disease ,Phenotype - Published
- 2021
32. Can Targeting the Incretin Pathway Dampen RAGE-Mediated Events in Diabetic Nephropathy?
- Author
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Mark E. Cooper, Henry Yao, Karly C. Sourris, Elif I Ekinci, George Jerums, and Melinda T. Coughlan
- Subjects
Glycation End Products, Advanced ,0301 basic medicine ,endocrine system ,medicine.medical_treatment ,Clinical Biochemistry ,Incretin ,Bioinformatics ,Incretins ,Glucagon-Like Peptide-1 Receptor ,RAGE (receptor) ,Renin-Angiotensin System ,Diabetic nephropathy ,03 medical and health sciences ,Glucagon-Like Peptide 1 ,Diabetes mellitus ,Drug Discovery ,medicine ,Animals ,Humans ,Diabetic Nephropathies ,Molecular Targeted Therapy ,Glucagon-like peptide 1 receptor ,Pharmacology ,business.industry ,Insulin ,digestive, oral, and skin physiology ,medicine.disease ,Glucagon-like peptide-1 ,Angiotensin II ,030104 developmental biology ,Drug Design ,Immunology ,Disease Progression ,Molecular Medicine ,Reactive Oxygen Species ,business ,hormones, hormone substitutes, and hormone antagonists - Abstract
Diabetic nephropathy is the major cause of end-stage renal disease in Western societies. To date, interruption of the Renin-Angiotensin System is the most effective intervention for diabetic nephropathy, however these agents only slow progression of the disease. Thus, there is a major unmet need for new therapeutic targets. Aberrant activation of the receptor for advanced glycation end products (RAGE) is involved in the pathogenesis of diabetic nephropathy via binding to a variety of ligands and inciting reactive oxygen species (ROS) production, inflammation and fibrosis. In recent years there have been considerable efforts in the development of effective RAGE antagonists, however, direct RAGE targeting may be problematic. Glucagon like peptide-1 (GLP-1) is an incretin hormone released by the L-cells of the small intestine to mediate glucose-dependent insulin release from pancreatic islets. The incretin-based therapies, GLP-1 receptor agonists and dipeptidylpeptidase-4 (DPP4) inhibitors, are novel glucose-lowering agents used in type 2 diabetes. However, the extra pancreatic functions of GLP-1 have gained attention, including putative anti-apoptotic and anti-inflammatory properties. In rodent models of diabetes, incretin-based therapies are renoprotective. Interestingly, GLP-1 has been shown to interfere with the signalling and expression of RAGE. The current review aims to give an overview of the interactions between the RAGE and incretin pathways and to discuss the utility of targeting the GLP-1/incretin pathway in DN. It is possible that indirect targeting of RAGE through GLP-1 agonism will be of clinical benefit to patients with diabetic nephropathy.
- Published
- 2016
33. Mapping time-course mitochondrial adaptations in the kidney in experimental diabetes
- Author
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Mark E. Cooper, Brooke E. Harcourt, Vicki Thallas-Bonke, Karly C. Sourris, Sally A. Penfold, Nicole J Van Bergen, Josephine M. Forbes, Sih Min Tan, Melinda T. Coughlan, Gavin C Higgins, David R. Thorburn, Ian A. Trounce, and Tuong-Vi Nguyen
- Subjects
Male ,0301 basic medicine ,medicine.medical_specialty ,Time Factors ,Bioenergetics ,Mitochondrion ,Kidney ,DNA, Mitochondrial ,Mitochondrial Dynamics ,Mitochondrial Membrane Transport Proteins ,Diabetes Mellitus, Experimental ,Nephropathy ,Rats, Sprague-Dawley ,Diabetic nephropathy ,03 medical and health sciences ,Mitochondrial membrane transport protein ,Internal medicine ,medicine ,Albuminuria ,Animals ,biology ,Mitochondrial Permeability Transition Pore ,General Medicine ,medicine.disease ,Adaptation, Physiological ,Mitochondria ,Up-Regulation ,Oxidative Stress ,Kidney Tubules ,Phenotype ,030104 developmental biology ,Endocrinology ,medicine.anatomical_structure ,Mitochondrial permeability transition pore ,biology.protein ,Energy Metabolism ,Kidney disease - Abstract
Oxidative phosphorylation (OXPHOS) drives ATP production by mitochondria, which are dynamic organelles, constantly fusing and dividing to maintain kidney homoeostasis. In diabetic kidney disease (DKD), mitochondria appear dysfunctional, but the temporal development of diabetes-induced adaptations in mitochondrial structure and bioenergetics have not been previously documented. In the present study, we map the changes in mitochondrial dynamics and function in rat kidney mitochondria at 4, 8, 16 and 32 weeks of diabetes. Our data reveal that changes in mitochondrial bioenergetics and dynamics precede the development of albuminuria and renal histological changes. Specifically, in early diabetes (4 weeks), a decrease in ATP content and mitochondrial fragmentation within proximal tubule epithelial cells (PTECs) of diabetic kidneys were clearly apparent, but no changes in urinary albumin excretion or glomerular morphology were evident at this time. By 8 weeks of diabetes, there was increased capacity for mitochondrial permeability transition (mPT) by pore opening, which persisted over time and correlated with mitochondrial hydrogen peroxide (H2O2) generation and glomerular damage. Late in diabetes, by week 16, tubular damage was evident with increased urinary kidney injury molecule-1 (KIM-1) excretion, where an increase in the Complex I-linked oxygen consumption rate (OCR), in the context of a decrease in kidney ATP, indicated mitochondrial uncoupling. Taken together, these data show that changes in mitochondrial bioenergetics and dynamics may precede the development of the renal lesion in diabetes, and this supports the hypothesis that mitochondrial dysfunction is a primary cause of DKD.
- Published
- 2016
34. Mitochondrial Fission/Fusion and Disease
- Author
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Gavin C Higgins and Melinda T. Coughlan
- Subjects
Programmed cell death ,medicine.anatomical_structure ,mitochondrial fusion ,Cell ,Mitophagy ,Neurodegeneration ,DNAJA3 ,medicine ,Mitochondrial fission ,Mitochondrion ,Biology ,medicine.disease ,Cell biology - Abstract
The mitochondria are a multifaceted organelle, central to the function of all eukaryotic cells. Mitochondria are critical to cell metabolism and critical for regulation of intrinsic cell death pathways. This balance between life and death is reliant on the process of mitochondrial fission and fusion, constantly joining and separating to maintain the mitochondrial network within the cell and to facilitate cellular events such as mitosis, biogenesis and maintenance of mitochondrial integrity via mitophagy. The dynamic fluctuations in the architecture of these organelles is garnering increased attention across a broad range of disciplines and diseases. Such diseases include genetic disorders such as Charcot-Marie-Tooth disease type 2A and autosomal-dominant optic atrophy, as well as pathologies associated with mitochondrial dysfunction such as chronic neurodegeneration such as Parkinson disease, type 2 diabetes and various forms of chronic and acute kidney injury. Key Concepts Mitochondria undergo constant fission and fusion to maintain function and biogenesis. Impairment of mitochondrial dynamics can result in reduced oxidative phosphorylation and cell death. Impairment of mitochondrial dynamics is associated with diseases involving cells abundant in mitochondria. Mitochondrial fusion is associated with energy production. Mitochondrial fragmentation is associated with mitochondrial dysfunction and turnover by mitophagy. Keywords: mitochondria; fission; fusion; kidney disease; neurodegeneration; cardiovascular disease; cancer
- Published
- 2016
35. WITHDRAWN: Nutritional supplements for diabetes: Preface
- Author
-
Melinda T. Coughlan
- Subjects
Pediatrics ,medicine.medical_specialty ,Nutrition and Dietetics ,business.industry ,Endocrinology, Diabetes and Metabolism ,Diabetes mellitus ,Medicine ,business ,medicine.disease ,Food Science - Published
- 2018
36. Modulation of the Gut Microbiota by Resistant Starch as a Treatment of Chronic Kidney Diseases: Evidence of Efficacy and Mechanistic Insights
- Author
-
Matthew Snelson, Nicole J. Kellow, and Melinda T. Coughlan
- Subjects
0301 basic medicine ,food.ingredient ,medicine.medical_treatment ,Medicine (miscellaneous) ,Incretin ,030209 endocrinology & metabolism ,Review ,Pharmacology ,Gut flora ,Kidney ,03 medical and health sciences ,0302 clinical medicine ,food ,Medicine ,Animals ,Humans ,Microbiome ,Resistant starch ,Renal Insufficiency, Chronic ,030109 nutrition & dietetics ,Nutrition and Dietetics ,biology ,business.industry ,Prebiotic ,Starch ,medicine.disease ,biology.organism_classification ,Gastrointestinal Microbiome ,Disease Models, Animal ,medicine.anatomical_structure ,Prebiotics ,Dietary Supplements ,Disease Progression ,Dysbiosis ,business ,Food Science ,Kidney disease - Abstract
Chronic kidney disease (CKD) has been associated with changes in gut microbial ecology, or "dysbiosis," which may contribute to disease progression. Recent studies have focused on dietary approaches to favorably alter the composition of the gut microbial communities as a treatment method in CKD. Resistant starch (RS), a prebiotic that promotes proliferation of gut bacteria such as Bifidobacteria and Lactobacilli, increases the production of metabolites including short-chain fatty acids, which confer a number of health-promoting benefits. However, there is a lack of mechanistic insight into how these metabolites can positively influence renal health. Emerging evidence shows that microbiota-derived metabolites can regulate the incretin axis and mitigate inflammation via expansion of regulatory T cells. Studies from animal models and patients with CKD show that RS supplementation attenuates the concentrations of uremic retention solutes, including indoxyl sulfate and p-cresyl sulfate. Here, we present the current state of knowledge linking the microbiome to CKD, we explore the efficacy of RS in animal models of CKD and in humans with the condition, and we discuss how RS supplementation could be a promising dietary approach for slowing CKD progression.
- Published
- 2018
37. Use of Readily Accessible Inflammatory Markers to Predict Diabetic Kidney Disease
- Author
-
Lauren Winter, Lydia A. Wong, George Jerums, Jas-mine Seah, Michele Clarke, Sih Min Tan, Melinda T. Coughlan, Richard J. MacIsaac, and Elif I. Ekinci
- Subjects
lymphocytes ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,neutrophil–lymphocyte ratio ,Renal function ,030209 endocrinology & metabolism ,Type 2 diabetes ,030204 cardiovascular system & hematology ,lcsh:Diseases of the endocrine glands. Clinical endocrinology ,Gastroenterology ,Diabetic nephropathy ,03 medical and health sciences ,0302 clinical medicine ,neutrophils ,Internal medicine ,Diabetes mellitus ,Medicine ,Autoimmune disease ,lcsh:RC648-665 ,urogenital system ,business.industry ,diabetic nephropathy ,medicine.disease ,diabetic kidney disease ,inflammation ,Albuminuria ,medicine.symptom ,Metabolic syndrome ,business ,Kidney disease - Abstract
Diabetic kidney disease is a common complication of type 1 and type 2 diabetes and is the primary cause of end-stage renal disease in developed countries. Early detection of diabetic kidney disease will facilitate early intervention aimed at reducing the rate of progression to end-stage renal disease. Diabetic kidney disease has been traditionally classified based on the presence of albuminuria. More recently estimated glomerular filtration rate has also been incorporated into the staging of diabetic kidney disease. While albuminuric diabetic kidney disease is well described, the phenotype of non-albuminuric diabetic kidney disease is now widely accepted. An association between markers of inflammation and diabetic kidney disease has previously been demonstrated. Effector molecules of the innate immune system including C-reactive protein, interleukin-6, and tumor necrosis factor-α are increased in patients with diabetic kidney disease. Furthermore, renal infiltration of neutrophils, macrophages, and lymphocytes are observed in renal biopsies of patients with diabetic kidney disease. Similarly high serum neutrophil and low serum lymphocyte counts have been shown to be associated with diabetic kidney disease. The neutrophil-lymphocyte ratio is considered a robust measure of systemic inflammation and is associated with the presence of inflammatory conditions including the metabolic syndrome and insulin resistance. Cross-sectional studies have demonstrated a link between high levels of the above inflammatory biomarkers and diabetic kidney disease. Further longitudinal studies will be required to determine if these readily available inflammatory biomarkers can accurately predict the presence and prognosis of diabetic kidney disease, above and beyond albuminuria, and estimated glomerular filtration rate.
- Published
- 2018
38. SAT-302 INHIBITION OF COMPLEMENT C5A/C5A RECEPTOR 1 DECREASES RENAL INJURY IN DIABETIC KIDNEY DISEASE VIA METABOLIC REPROGRAMMING
- Author
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Sih Min Tan, P. Meikle, T. Woodruff, Mark Ziemann, Mark E. Cooper, Elif I Ekinci, Melinda T. Coughlan, and Richard J MacIsaac
- Subjects
medicine.medical_specialty ,Endocrinology ,Renal injury ,Diabetic kidney ,Nephrology ,business.industry ,Internal medicine ,Metabolic reprogramming ,medicine ,Complement C5a ,Disease ,business ,C5a receptor - Published
- 2019
39. Perinatal exposure to high dietary advanced glycation end products in transgenic NOD8.3 mice leads to pancreatic beta cell dysfunction
- Author
-
Danielle J. Borg, Melinda T. Coughlan, Sahar Keshvari, Phillip Kantharidis, Robyn Maree Slattery, Sumaira Z. Hasnain, Linda A. Gallo, David G. Simmons, Josephine M. Forbes, Amelia K. Fotheringham, Aowen Zhuang, and Felicia Y. T. Yap
- Subjects
0301 basic medicine ,Glycation End Products, Advanced ,Male ,Endocrinology, Diabetes and Metabolism ,insulitis ,Mice ,0302 clinical medicine ,Endocrinology ,Mice, Inbred NOD ,Pregnancy ,Lactation ,Medicine ,geography.geographical_feature_category ,islet ,advanced glycation end products ,Glucagon secretion ,NOD8.3 ,Islet ,medicine.anatomical_structure ,Type 1 diabetes ,Biochemistry ,Prenatal Exposure Delayed Effects ,Female ,Research Paper ,medicine.medical_specialty ,endocrine system ,insulin ,Offspring ,030209 endocrinology & metabolism ,Mice, Transgenic ,Glucagon ,03 medical and health sciences ,Islets of Langerhans ,dietary intervention ,Internal medicine ,Weaning ,Animals ,geography ,business.industry ,Maternal Nutritional Physiological Phenomena ,medicine.disease ,Diet ,Mice, Inbred C57BL ,030104 developmental biology ,Diabetes Mellitus, Type 1 ,Animals, Newborn ,business ,Insulitis - Abstract
The contribution of environmental factors to pancreatic islet damage in type 1 diabetes remains poorly understood. In this study, we crossed mice susceptible to type 1 diabetes, where parental male (CD8+ T cells specific for IGRP206-214; NOD8.3) and female (NOD/ShiLt) mice were randomized to a diet either low or high in AGE content and maintained on this diet throughout pregnancy and lactation. After weaning, NOD8.3+ female offspring were identified and maintained on the same parental feeding regimen for until day 28 of life. A low AGE diet, from conception to early postnatal life, decreased circulating AGE concentrations in the female offspring when compared to a high AGE diet. Insulin, proinsulin and glucagon secretion were greater in islets isolated from offspring in the low AGE diet group, which was akin to age matched non-diabetic C57BL/6 mice. Pancreatic islet expression of Ins2 gene was also higher in offspring from the low AGE diet group. Islet expression of glucagon, AGEs and the AGE receptor RAGE, were each reduced in low AGE fed offspring. Islet immune cell infiltration was also decreased in offspring exposed to a low AGE diet. Within pancreatic lymph nodes and spleen, the proportions of CD4+ and CD8+ T cells did not differ between groups. There were no significant changes in body weight, fasting glucose or glycemic hormones. This study demonstrates that reducing exposure to dietary AGEs throughout gestation, lactation and early postnatal life may benefit pancreatic islet secretion and immune infiltration in the type 1 diabetic susceptible mouse strain, NOD8.3.
- Published
- 2017
40. Effect of diet-derived advanced glycation end products on inflammation
- Author
-
Melinda T. Coughlan and Nicole J. Kellow
- Subjects
Glycation End Products, Advanced ,medicine.medical_specialty ,Metabolite ,Medicine (miscellaneous) ,Incretin ,Inflammation ,Gut flora ,Pathogenesis ,symbols.namesake ,chemistry.chemical_compound ,Glycation ,Internal medicine ,medicine ,Humans ,Receptor ,Nutrition and Dietetics ,biology ,biology.organism_classification ,Maillard Reaction ,Maillard reaction ,Endocrinology ,chemistry ,Food ,Immunology ,symbols ,medicine.symptom - Abstract
Advanced glycation end products (AGEs) formed via the Maillard reaction during the thermal processing of food contributes to the flavor, color, and aroma of food. A proportion of food-derived AGEs and their precursors is intestinally absorbed and accumulates within cells and tissues. AGEs have been implicated in the pathogenesis of diabetes-related complications and several chronic diseases via interaction with the receptor for AGEs, which promotes the transcription of genes that control inflammation. The dicarbonyls, highly reactive intermediates of AGE formation, are also generated during food processing and may incite inflammatory responses through 1) the suppression of protective pathways, 2) the incretin axis, 3) the modulation of immune-mediated signaling, and 4) changes in gut microbiota profile and metabolite sensors. In animal models, restriction of dietary AGEs attenuates chronic low-grade inflammation, but current evidence from human studies is less clear. Here, the emerging relationship between excess dietary AGE consumption and inflammation is explored, the utility of dietary AGE restriction as a therapeutic strategy for the attenuation of chronic diseases is discussed, and possible avenues for future investigation are suggested.
- Published
- 2015
41. Increased liver AGEs induce hepatic injury mediated through an OST48 pathway
- Author
-
Domenica A. McCarthy, Aowen Zhuang, Christopher Leung, Mark A. Febbraio, Felicia Y. T. Yap, Josephine M. Forbes, Karly C. Sourris, Phillip Kantharidis, Matthew J. Watt, Peter W Angus, Clinton R. Bruce, Chandana B Herath, Manuel R. Plan, Benjamin L. Schulz, Mitchell A. Sullivan, Melinda T. Coughlan, and Mark P. Hodson
- Subjects
Glycation End Products, Advanced ,Liver Cirrhosis ,Male ,0301 basic medicine ,medicine.medical_specialty ,medicine.medical_treatment ,Receptor for Advanced Glycation End Products ,lcsh:Medicine ,Mice, Transgenic ,medicine.disease_cause ,Article ,Mice ,03 medical and health sciences ,Glycation ,Internal medicine ,medicine ,Animals ,Humans ,lcsh:Science ,Liver injury ,Multidisciplinary ,business.industry ,Insulin ,Endoplasmic reticulum ,lcsh:R ,Membrane Proteins ,Feeding Behavior ,Endoplasmic Reticulum Stress ,medicine.disease ,Mice, Inbred C57BL ,Disease Models, Animal ,Oxidative Stress ,030104 developmental biology ,Endocrinology ,Hexosyltransferases ,Liver ,Unfolded protein response ,lcsh:Q ,Steatosis ,Hepatic fibrosis ,business ,Oxidative stress ,Signal Transduction - Abstract
The protein oligosaccharyltransferase-48 (OST48) is integral to protein N-glycosylation in the endoplasmic reticulum (ER) but is also postulated to act as a membrane localised clearance receptor for advanced glycation end-products (AGE). Hepatic ER stress and AGE accumulation are each implicated in liver injury. Hence the objective of this study was to increase the expression of OST48 and examine the effects on hepatic function and structure. Groups of 8 week old male mice (n = 10–12/group) over-expressing the gene for OST48, dolichyl-diphosphooligosaccharide-protein glycosyltransferase (DDOST+/−), were followed for 24 weeks, while randomised to diets either low or high in AGE content. By week 24 of the study, either increasing OST48 expression or consumption of high AGE diet impaired liver function and modestly increased hepatic fibrosis, but their combination significantly exacerbated liver injury in the absence of steatosis. DDOST+/− mice had increased both portal delivery and accumulation of hepatic AGEs leading to central adiposity, insulin secretory defects, shifted fuel usage to fatty and ketoacids, as well as hepatic glycogen accumulation causing hepatomegaly along with hepatic ER and oxidative stress. This study revealed a novel role of the OST48 and AGE axis in hepatic injury through ER stress, changes in fuel utilisation and glucose intolerance.
- Published
- 2017
42. Obesity associated advanced glycation end products within the human uterine cavity adversely impact endometrial function and embryo implantation competence
- Author
-
Lois A. Salamonsen, Gabriella S Antoniotti, Melinda T. Coughlan, and Jemma Evans
- Subjects
0301 basic medicine ,Adult ,Glycation End Products, Advanced ,Stromal cell ,Endometrium ,Cell morphology ,Andrology ,03 medical and health sciences ,0302 clinical medicine ,Pregnancy ,Medicine ,Humans ,Blastocyst ,Embryo Implantation ,Obesity ,Endometrial Stromal Cell ,030219 obstetrics & reproductive medicine ,business.industry ,Rehabilitation ,Uterus ,Obstetrics and Gynecology ,Trophoblast ,Decidualization ,Epithelial Cells ,Placentation ,Trophoblasts ,030104 developmental biology ,medicine.anatomical_structure ,Reproductive Medicine ,Female ,Uterine cavity ,Stromal Cells ,business - Abstract
STUDY QUESTION Do obese levels of advanced glycation end products (AGEs) within the uterine cavity detrimentally alter tissue function in embryo implantation and placental development? SUMMARY ANSWER Obese levels of AGEs activate inflammatory signaling (p65 NFκB) within endometrial epithelial cells and alter their function, cause endoplasmic reticulum (ER) stress in endometrial stromal cells and impair decidualization, compromise implantation of blastocyst mimics and inhibit trophoblast invasion. WHAT IS KNOWN ALREADY Obese women experience a higher incidence of infertility, recurrent miscarriage and pregnancy complications compared with lean women. Oocyte donation cycles suggest a detrimental uterine environment plays a role in these outcomes. STUDY DESIGN, SIZE, DURATION Uterine lavage and tissues from lean (BMI 19.5-24.9, n = 17) and obese (BMI > 30, n = 16) women examined. Cell culture experiments utilizing human endometrial epithelial, trophectoderm and trophoblast cell lines and primary human stromal cells used to examine the functional impact of obese levels of AGEs. PARTICIPANTS/MATERIALS, SETTING, METHODS Levels of AGEs examined within uterine lavage assessed by ELISA to determine differences between lean and obese women. Expression and localization of AGEs, receptor for AGEs (RAGE) and NFκB within endometrial tissues obtained from lean and obese women determined by immunohistochemistry. Endometrial epithelial cells (ECC-1), primary human stromal cells and trophoblast cells (HTR8-SVneo) treated with lean (2000 nmol/mol lysine) or obese (8000 nmol/mol lysine) uterine levels of AGEs and p65 NFκB (western immunoblot), real-time adhesion, proliferation migration and invasion (xCelligence real-time cell function analysis), decidualization (cell morphology and prolactin release), ER stress (western immunoblot for p-PERK) determined. Co-cultures of endometrial epithelial cells and blastocyst mimics (trophectoderm spheroids) similarly treated with lean or obese uterine levels of AGEs to determine their impact on embryo implantation. MAIN RESULTS AND THE ROLE OF CHANCE AGEs were significantly elevated (P = 0.004) within the obese (6503.59 μmol/mol lysine) versus lean (2165.88 μmol/mol lysine) uterine cavity (uterine lavage) with increased immunostaining for AGEs, RAGE and NFkB within obese endometrial tissues during the proliferative phase of the menstrual cycle. Obese uterine levels of AGEs inhibited adhesion and proliferation of endometrial epithelial (ECC-1) cells compared to treatment with lean uterine levels of AGEs. Obese uterine AGE levels impacted primary human endometrial stromal cell decidualization and activated ER stress within these cells. Obese uterine levels of AGEs also inhibited trophectodermal spheroid adhesion to hormonally primed endometrial epithelial cells and trophoblast cell line HTR8/SV-neo invasion. LARGE SCALE DATA N/A. LIMITATIONS REASONS FOR CAUTION Mechanistic studies are performed in vitro and may not completely recapitulate cell function in vivo. WIDER IMPLICATIONS OF THE FINDINGS These data corroborate clinical data suggesting the presence of an altered uterine environment in obese women and demonstrate that elevated uterine levels of AGEs within these women may detrimentally impact endometrial function, embryo implantation and placental development. Uterine AGE assessment in infertility work up may prove useful in determining underlying causes of infertility. AGEs can be targeted pharmacologically and such treatments may prove effective in improving reproductive complications experience by obese women. STUDY FUNDING/COMPETING INTEREST(S) Supported by NHMRC Fellowship (#1002028 to L.A.S.), and the Victorian Government's Operational Infrastructure Support Program. MTC is supported by a JDRF Australia Clinical Research Network Career Development Award. The authors have declared that no conflict of interest exists.
- Published
- 2017
43. Mitochondrial dysfunction and mitophagy: the beginning and end to diabetic nephropathy?
- Author
-
Gavin C Higgins and Melinda T. Coughlan
- Subjects
Pharmacology ,medicine.medical_specialty ,Kidney ,Renal cortex ,Mitochondrial Degradation ,Mitochondrion ,Biology ,medicine.disease ,Diabetic nephropathy ,Endocrinology ,medicine.anatomical_structure ,Renal pathology ,Renal physiology ,Internal medicine ,Mitophagy ,Cancer research ,medicine - Abstract
Diabetic nephropathy (DN) is a progressive microvascular complication arising from diabetes. Within the kidney, the glomeruli, tubules, vessels and interstitium are disrupted, ultimately impairing renal function and leading to end-stage renal disease (ESRD). Current pharmacological therapies used in individuals with DN do not prevent the inevitable progression to ESRD; therefore, new targets of therapy are urgently required. Studies from animal models indicate that disturbances in mitochondrial homeostasis are central to the pathogenesis of DN. Since renal proximal tubule cells rely on oxidative phosphorylation to provide adequate ATP for tubular reabsorption, an impairment of mitochondrial bioenergetics can result in renal functional decline. Defects at the level of the electron transport chain have long been established in DN, promoting electron leakage and formation of superoxide radicals, mediating microinflammation and contributing to the renal lesion. More recent studies suggest that mitochondrial-associated proteins may be directly involved in the pathogenesis of tubulointerstitial fibrosis and glomerulosclerosis. An accumulation of fragmented mitochondria are found in the renal cortex in both humans and animals with DN, suggesting that in tandem with a shift in dynamics, mitochondrial clearance mechanisms may be impaired. The process of mitophagy is the selective targeting of damaged or dysfunctional mitochondria to autophagosomes for degradation through the autophagy pathway. The current review explores the concept that an impairment in the mitophagy system leads to the accelerated progression of renal pathology. A better understanding of the cellular and molecular events that govern mitophagy and dynamics in DN may lead to improved therapeutic strategies. Linked Articles This article is part of a themed issue on Mitochondrial Pharmacology: Energy, Injury & Beyond. To view the other articles in this issue visit http://dx.doi.org/10.1111/bph.2014.171.issue-8
- Published
- 2014
44. SAT-105 GLUCOSE-DEPENDENT MITOCHONDRIAL ALTERATIONS IN DIABETIC KIDNEY DISEASE
- Author
-
Mark E. Cooper, Vicki Thallas-Bonke, G. Ramm, D. Stroud, R. Ritchie, C. Granata, C.X. Qin, Melinda T. Coughlan, A. Laskowski, and E. Jap
- Subjects
medicine.medical_specialty ,Endocrinology ,Diabetic kidney ,Nephrology ,business.industry ,Internal medicine ,medicine ,Disease ,business - Published
- 2019
45. SAT-301 RESISTANT STARCH AMELIORATES ADVANCED GLYCATION ENDPRODUCT-INDUCED GUT DYSBIOSIS AND ALBUMINURIA IN A MOUSE MODEL OF TYPE 2 DIABETES
- Author
-
Mark Ziemann, Melinda T. Coughlan, Sih Min Tan, Vicki Thallas-Bonke, Karly C. Sourris, Mark E. Cooper, Matthew Snelson, and S. El-Osta
- Subjects
medicine.medical_specialty ,food.ingredient ,business.industry ,Type 2 diabetes ,medicine.disease ,Endocrinology ,food ,Nephrology ,Glycation ,Internal medicine ,medicine ,Albuminuria ,Gut dysbiosis ,medicine.symptom ,Resistant starch ,business - Published
- 2019
46. Complement C5a receptor 1 (C5aR1) modulates mitochondrial fatty acid oxidation and cardiolipin remodelling leading to diabetic kidney disease
- Author
-
Elif I Ekinci, Sih Min Tan, Darren C. Henstridge, Melinda T. Coughlan, Sam El-Osta, Mark Ziemann, Peter J. Meikle, and Trent M. Woodruff
- Subjects
medicine.medical_specialty ,Nutrition and Dietetics ,Diabetic kidney ,Endocrinology, Diabetes and Metabolism ,Complement C5a ,Disease ,Mitochondrial fatty acid ,chemistry.chemical_compound ,Endocrinology ,chemistry ,Internal medicine ,medicine ,Cardiolipin ,Receptor - Published
- 2019
47. Nutritional supplements for diabetes
- Author
-
Melinda T. Coughlan
- Subjects
medicine.medical_specialty ,Nutrition and Dietetics ,business.industry ,Endocrinology, Diabetes and Metabolism ,Diabetes mellitus ,Internal medicine ,medicine ,medicine.disease ,business ,Food Science - Published
- 2018
48. Young Investigator Award
- Author
-
Kathryn White, Georg Ramm, Elif I Ekinci, Portia M Robb, Melinda T. Coughlan, David A. Power, Tuong-Vi Nguyen, Alison Skene, Rsj Lindblom, KS Sourris, Sih Min Tan, Gavin C Higgins, Thallas-Bonke, JB Dehaan, Rudolf W. Bilous, George Jerums, Richard J MacIsaac, Sally A. Penfold, and Mark E. Cooper
- Subjects
Diabetic nephropathy ,medicine.medical_specialty ,Endocrinology ,Nephrology ,business.industry ,Internal medicine ,Autophagy ,medicine ,General Medicine ,business ,medicine.disease - Published
- 2015
49. Deficiency in Mitochondrial Complex I Activity Due toNdufs6Gene Trap Insertion Induces Renal Disease
- Author
-
Karly C. Sourris, Tuong-Vi Nguyen, Josephine M. Forbes, Adrienne Laskowski, Darren C. Henstridge, Lukas N. Groschner, David R. Thorburn, Sally A. Penfold, Melinda T. Coughlan, Mark E. Cooper, and Bi-Xia Ke
- Subjects
medicine.medical_specialty ,Mitochondrial Diseases ,Physiology ,Clinical Biochemistry ,Oxidative phosphorylation ,Gene mutation ,urologic and male genital diseases ,Biochemistry ,Antioxidants ,Mice ,chemistry.chemical_compound ,Adenosine Triphosphate ,Internal medicine ,medicine ,Renal fibrosis ,Animals ,Molecular Biology ,General Environmental Science ,Mice, Knockout ,NDUFS6 ,Gene knockdown ,Electron Transport Complex I ,biology ,Superoxide Dismutase ,Superoxide ,NADH Dehydrogenase ,Cell Biology ,Endocrinology ,Mitochondrial respiratory chain ,Cystatin C ,chemistry ,biology.protein ,General Earth and Planetary Sciences ,Kidney Diseases ,Reactive Oxygen Species - Abstract
Defects in the activity of enzyme complexes of the mitochondrial respiratory chain are thought to be responsible for several disorders, including renal impairment. Gene mutations that result in complex I deficiency are the most common oxidative phosphorylation disorders in humans. To determine whether an abnormality in mitochondrial complex I per se is associated with development of renal disease, mice with a knockdown of the complex I gene, Ndufs6 were studied.Ndufs6 mice had a partial renal cortical complex I deficiency; Ndufs6gt/gt, 32% activity and Ndufs6gt/+, 83% activity compared with wild-type mice. Both Ndufs6gt/+ and Ndufs6gt/gt mice exhibited hallmarks of renal disease, including albuminuria, urinary excretion of kidney injury molecule-1 (Kim-1), renal fibrosis, and changes in glomerular volume, with decreased capacity to generate mitochondrial ATP and superoxide from substrates oxidized via complex I. However, more advanced renal defects in Ndufs6gt/gt mice were observed in the context of a disruption in the inner mitochondrial electrochemical potential, 3-nitrotyrosine-modified mitochondrial proteins, increased urinary excretion of 15-isoprostane F2t, and up-regulation of antioxidant defence. Juvenile Ndufs6gt/gt mice also exhibited signs of early renal impairment with increased urinary Kim-1 excretion and elevated circulating cystatin C.We have identified renal impairment in a mouse model of partial complex I deficiency, suggesting that even modest deficits in mitochondrial respiratory chain function may act as risk factors for chronic kidney disease.These studies identify for the first time that complex I deficiency as the result of interruption of Ndufs6 is an independent cause of renal impairment.
- Published
- 2013
50. Advanced glycation end products (AGEs) are cross-sectionally associated with insulin secretion in healthy subjects
- Author
-
Josephine M. Forbes, Markus P. Schlaich, Mark E. Cooper, Bronwyn A. Kingwell, Karly C. Sourris, Nora E. Straznicky, Barbora de Courten, Vibhasha Chand, Maximilian de Courten, Melinda T. Coughlan, Jasmine G. Lyons, Georgia Soldatos, Sonia L Dougherty, David A. Bertovic, Forbes, Josephine, Sourris, Karly, De Courten, Maximilian PJ, Dougherty, Sonia, Chand, Vibhasha, Lyons, Jasmine Genevieve, Bertovic, David, Coughlan, Melinda, Schlaich, Markus Peter, Soldatos, Georgia, Cooper, Mark, Straznicky, Nora, Kingwell, B.A, and De Courten, Barbora Vozarova
- Subjects
Adult ,Blood Glucose ,Glycation End Products, Advanced ,Male ,insulin secretion ,medicine.medical_specialty ,medicine.medical_treatment ,Receptor for Advanced Glycation End Products ,Clinical Biochemistry ,Type 2 diabetes ,Biochemistry ,RAGE (receptor) ,Young Adult ,Insulin resistance ,Glycation ,Internal medicine ,Insulin Secretion ,medicine ,Humans ,Insulin ,insulin sensitivity ,Receptors, Immunologic ,Young adult ,Insulin secretion ,Receptor ,central obesity ,business.industry ,Organic Chemistry ,medicine.disease ,Cross-Sectional Studies ,Endocrinology ,Female ,type 2 diabetes ,Insulin Resistance ,business - Abstract
It has been postulated that chronic exposure to high levels of advanced glycation end products (AGEs), in particular from dietary sources, can impair insulin secretion. In the present study, we investigated the cross-sectional relationship between AGEs and acute insulin secretion during an intravenous glucose tolerance test (IVGTT) and following a 75 g oral glucose tolerance test (OGTT) in healthy humans. We report the cross-sectional association between circulating AGE concentrations and insulin secretory function in healthy humans (17 F: 27 M, aged 30 ± 10 years) with a wide range of BMI (24.6–31.0 kg/m2). Higher circulating concentrations of AGEs were related to increased first phase insulin secretion during IVGTT (r = 0.43; p < 0.05) and lower 2-h glucose concentrations during OGTT (r = −0.31; p < 0.05). In addition, fasting (r = −0.36; p < 0.05) and 2-h glucose concentrations were negatively related to circulating levels of soluble receptor for AGE (RAGE) isoforms (r = −0.39; p < 0.01). In conclusion, in healthy humans, we show a cross-sectional association between advanced glycation end products and acute insulin secretion during glucose tolerance testing. Refereed/Peer-reviewed
- Published
- 2013
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