1. The Antiseptic Octenidine Inhibits Langerhans Cell Activation and Modulates Cytokine Expression upon Superficial Wounding with Tape Stripping
- Author
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Nenad Nikolić, Philip Kienzl, Johannes Matiasek, Adelheid Elbe-Bürger, Martin Vierhapper, and Pooja Tajpara
- Subjects
Adult ,lcsh:Immunologic diseases. Allergy ,Pathology ,medicine.medical_specialty ,Langerhans cell ,Article Subject ,Pyridines ,Angiogenesis ,Immunology ,Human skin ,Models, Biological ,Young Adult ,030207 dermatology & venereal diseases ,03 medical and health sciences ,Wound care ,0302 clinical medicine ,Anti-Infective Agents ,Humans ,Immunology and Allergy ,Medicine ,Surgical Tape ,Skin ,030304 developmental biology ,Wound Healing ,0303 health sciences ,integumentary system ,Epidermis (botany) ,business.industry ,General Medicine ,Middle Aged ,3. Good health ,medicine.anatomical_structure ,Epidermal Cells ,Langerhans Cells ,Cytokines ,Wounds and Injuries ,Cytokine secretion ,Imines ,Epidermis ,lcsh:RC581-607 ,business ,Wound healing ,Ex vivo ,Research Article - Abstract
Ideal agents for the topical treatment of skin wounds should have antimicrobial efficacy without negative influence on wound healing. Octenidine (OCT) has become a widely used antiseptic in professional wound care, but its influence on several components of the wound healing process remains unclear. In the present study, we have used a superficial wound model using tape stripping on human full-thickness skin ex vivo to investigate the influence of OCT on epidermal Langerhans cells (LCs) and cytokine secretion pattern of skin cells during wound healing in a model without disruption of the normal skin structure. Histological and immunofluorescence studies showed that OCT neither altered human skin architecture nor the viability of skin cells upon 48 hours of culture in unwounded or wounded skin. The epidermis of explants and LCs remained morphologically intact throughout the whole culture period upon OCT treatment. OCT inhibited the upregulation of the maturation marker CD83 on LCs and prevented their emigration in wounded skin. Furthermore, OCT reduced both pro- and anti-inflammatory mediators (IL-8, IL-33, and IL-10), while angiogenesis and growth factor mediators (VEGF and TGF-β1) remained unchanged in skin explant cultures. Our data provide novel insights into the host response to OCT in the biologically relevant environment of viable human (wounded) skin.
- Published
- 2019