57 results on '"Amira S. Wanas"'
Search Results
2. Antifungal guanidine alkaloids from the marine sponges Monanchora clathrata and Monanchora unguiculata
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Ali E. Raslan, Mohamed M. Radwan, Safwat A. Ahmed, Alaa M. Nafady, Ahmed M. Metwaly, Ibrahim H. Eissa, Melissa R. Jacob, Hashim A. Hassanean, Mahmoud A. ElSohly, and Amira S. Wanas
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Plant Science ,Agronomy and Crop Science ,Biochemistry ,Biotechnology - Published
- 2022
3. 7 Identification and analysis of cannabis
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Mahmoud A. ElSohly, Amira S. Wanas, Mohamed M. Radwan, Suman Chandra, and Chandrani G. Majumdar
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- 2023
4. Quantitative Determination of Cannabis Terpenes Using Gas Chromatography-Flame Ionization Detector
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Elsayed A. Ibrahim, Mohamed M. Radwan, Waseem Gul, Chandrani G. Majumdar, Ghada M. Hadad, Randa A. Abdel Salam, Amany K. Ibrahim, Safwat A. Ahmed, Suman Chandra, Hemant Lata, Mahmoud A. ElSohly, and Amira S. Wanas
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Pharmacology ,Complementary and alternative medicine ,Pharmacology (medical) - Published
- 2022
5. Antimicrobial, antimalarial and antileishmanial activities of Abutilon hirtum
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Amira S. Wanas, Samar Yehia Desoukey, Mohamed Kamel, Rehab Mahmoud Abd-Elbaky, and Alshymaa Abdel-Rahman Gomaa
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Abutilon ,biology ,Antiparasitic ,medicine.drug_class ,medicine.disease_cause ,biology.organism_classification ,medicine.disease ,Antimicrobial ,Microbiology ,Sierra leone ,chemistry.chemical_compound ,chemistry ,Staphylococcus aureus ,medicine ,Petroleum ether ,Klebsiella pneumonia ,Candida albicans - Abstract
The total ethanolic extract, different fractions and crude polysaccharides of leaves of Abutilon hirtum (Lam.) Sweet, were evaluated for their antimicrobial and antiparasitic activities. The antimicrobial activity was determined using the disc diffusion method against Staphylococcus aureus (Gram-positive, Facultative anaerobic bacteria), Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa (Gram-negative, Facultative anaerobic bacteria), Candida albicans, C. krusei (Diploid fungi) and C. glabrata (haploid fungus). The antimalarial activity was examined on chloroquine-sensitive (D6, Sierra Leone) strain of Plasmodium falciparum protozoan and the antileishmanial activity was tested against Leishmania donovani. The results of the present study showed that the total extract exhibited the lowest MIC (11.8 μg/ml) against P. aeruginosa. While, the chloroform fraction showed low MIC (59.03, 181.72 and 364.03 μg/ml) against S. aureus, K. pneumoniae and P. aeruginosa, respectively. The petroleum ether fraction exhibited the lowest MIC value (3.00 μg/ml) against C. albicans, while the total extract showed the lowest MIC (33.11 μg/ml) against C. glabrata. A. hirtum leaves extract and fractions showed a weak antimalarial activity. On the other hand, the chloroform fraction of A. hirtum leaves exhibited a potent antileishmanial activity with the highest percentage of inhibition (78%) against Leishmania donovani AMASTTHP_Pinh.
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- 2021
6. In vivo anti-inflammatory and hepatoprotective activities of Orobanche crenata (Forssk.) aerial parts in relation to its phytomolecules
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Amira S. Wanas, Ahmed M.M. Hassanein, Mohammed A A Orabi, Heba M. Tawfik, Samar Y. Desoukey, and Safwat M S Abo-Qotb
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Stigmasterol ,biology ,Traditional medicine ,010405 organic chemistry ,Chemistry ,Organic Chemistry ,Plant Science ,Orobanche crenata ,biology.organism_classification ,Chrysoeriol ,01 natural sciences ,Biochemistry ,0104 chemical sciences ,Analytical Chemistry ,Tyrosol ,010404 medicinal & biomolecular chemistry ,chemistry.chemical_compound ,Verbascoside ,Phytochemical ,Apigenin ,Luteolin - Abstract
A total methanolic extract and its sub-extracts of Orobanche crenata (Forssk.) aerial parts were subjected to acute toxicity, anti-inflammatory, and hepatoprotective investigations. The methanolic extract was safe upto 3 g/kg on mice. The EtOAc fraction reduced the carrageenan-induced rat paw edema better than indomethacin. It also demonstrated a drop in the elevated ALT, AST, and TB at 300 mg/kg, better than silymarin. Histopathological examination of liver cells of rats given the EtOAc fraction showed a complete absence of the CCl4-induced cloudy swelling. A phytochemical investigation of the n-hexane and EtOAc fractions yielded 11 compounds [indole-3-carboxylic acid (1), n-butyl palmitate (2), tyrosol (3), L-rhamnonic acid-1,4-lactone (4), β-sitosterol/stigmasterol mixture (5/5'), β-sitosterol/stigmasterol glycosides mixture (6/6'), chrysoeriol (7), luteolin (8), apigenin (9), crenatoside (10), and verbascoside (11)] as identified by UV, 1D & 2D NMR and ESIMS techniques. Their reported biological actions were in relation to and supported our herein detected pharmacological findings.
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- 2020
7. Absorbance-Transmittance Excitation Emission Matrix Method for Quantification of Major Cannabinoids and Corresponding Acids: A Rapid Alternative to Chromatography for Rapid Chemotype Discrimination of
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Adam M, Gilmore, Mostafa A, Elhendawy, Mohamed M, Radwan, Linda H, Kidder, Amira S, Wanas, Murrell, Godfrey, Jana B, Hildreth, A Edward, Robinson, and Mahmoud A, ElSohly
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- 2022
8. Absorbance-Transmittance Excitation Emission Matrix Method for Quantification of Major Cannabinoids and Corresponding Acids: A Rapid Alternative to Chromatography for Rapid Chemotype Discrimination of Cannabis sativa Varieties
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Adam M. Gilmore, Mostafa A. Elhendawy, Mohamed M. Radwan, Linda H. Kidder, Amira S. Wanas, Murrell Godfrey, Jana B. Hildreth, A. Edward Robinson, and Mahmoud A. ElSohly
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Pharmacology ,Complementary and alternative medicine ,Pharmacology (medical) - Published
- 2022
9. Phytochemical and pharmacological appraisal of the aerial parts of Lotus corniculatus L. growing in Egypt
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Hala M. Hammoda, Mohamed M. Radwan, Nahla S El-Gazzar, Amira S. Wanas, Mahmoud A. ElSohly, Maha A El-Demellawy, Rokia M. Abdallah, Shaimaa M. Sallam, and Nevin M Abdel-Rahman
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Traditional medicine ,010405 organic chemistry ,medicine.drug_class ,Organic Chemistry ,Plant Science ,Fabaceae ,Biology ,biology.organism_classification ,01 natural sciences ,Biochemistry ,0104 chemical sciences ,Analytical Chemistry ,010404 medicinal & biomolecular chemistry ,Phytochemical ,Antiprotozoal ,medicine ,Lotus corniculatus - Abstract
Lotus corniculatus L. (Fabaceae) is widely grown in Egypt. It has a great history of folkloric medicinal uses. All fractions of aerial parts of L. corniculatus L. showed significant antioxidant and...
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- 2020
10. Isolation of Sesquiterpene–Amino Acid Conjugates, Onopornoids A–D, and a Flavonoid Glucoside from Onopordum alexandrinum
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Yoshi Yamano, Kazuaki Katakawa, Samar Y. Desoukey, Katsuyoshi Matsunami, Sachiko Sugimoto, Hideaki Otsuka, and Amira S. Wanas
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Onopordum ,Stereochemistry ,Phytochemicals ,Flavonoid ,Pharmaceutical Science ,Sesquiterpene ,01 natural sciences ,Pyrrolidine ,Analytical Chemistry ,chemistry.chemical_compound ,Glucosides ,Glucoside ,Drug Discovery ,Amino Acids ,Flavonoids ,Pharmacology ,chemistry.chemical_classification ,Molecular Structure ,biology ,010405 organic chemistry ,Chemistry ,Organic Chemistry ,biology.organism_classification ,0104 chemical sciences ,Amino acid ,010404 medicinal & biomolecular chemistry ,Complementary and alternative medicine ,Phytochemical ,Molecular Medicine ,Egypt ,Sesquiterpenes ,Conjugate - Abstract
Previous phytochemical investigations have revealed the presence of a variety of compounds such as pyrrolidine derivatives, flavonoids, and megastigmanes in Egyptian plants. Onopordum alexandrinum has been traditionally used by the natives for treatment of skin cancers and leprosy. In this paper the isolation of four new sesquiterpene-amino acid conjugates, onopornoids A-D (1-4), i.e., three elemanes and one germacrane, and a new acylated flavonoid glucoside (5) along with nine known compounds (6-14) from the whole aerial parts of the title plant is discussed. The structures were elucidated based on chemical and spectroscopic/spectrometric data.
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- 2019
11. Metabolomic profiling and anti-infective potential of Zinnia elegans and Gazania rigens (Family Asteraceae)
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Samar Yehia Desoukey, Markus Krischke, Usama Ramadan Abdelmohsen, Mohamed Kamel, Mamdouh Nabil Samy, Alshymaa Abdel-Rahman Gomaa, Amira S. Wanas, and Martin J. Mueller
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biology ,010405 organic chemistry ,Organic Chemistry ,Gazania rigens ,Ethyl acetate ,Zinnia elegans ,Plasmodium falciparum ,Plant Science ,Phenolic acid ,biology.organism_classification ,Sesquiterpene ,01 natural sciences ,Biochemistry ,0104 chemical sciences ,Analytical Chemistry ,010404 medicinal & biomolecular chemistry ,chemistry.chemical_compound ,Metabolomics ,chemistry ,Liquid chromatography–mass spectrometry ,Botany - Abstract
The present study evaluates the chemical composition of Zinnia elegans and Gazania rigens based on their metabolomic profiles using liquid chromatography coupled with high-resolution mass spectrometry (LC-HR-MS), alongside with the anti-infective activities of their ethanol extracts, as well as, different fractions. A significant difference was observed between the LC-MS profiles of the two plants such as, coumarins, sesquiterpene lactones and phenylethanoids which were characteristic for Z. elegans, while amides and phenolic acid derivatives were characteristic for G. rigens. These results highlight the chemical potential of Z. elegans and G. rigens. Furthermore, the ethyl acetate fraction of Z. elegans showed a significant antimalarial activity with IC50 values of 21.03 and 13.72 µg/mL against Plasmodium falciparum D6 and P. falciparum W2, respectively.
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- 2018
12. Chemical and Biological Studies of Cannabis sativa Roots
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Mostafa A. Elhendawy, Amira S. Wanas, Mahmoud A. ElSohly, Mohamed M. Radwan, N. A. Azzaz, and ElShahat S Toson
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Pharmacology ,Cryptococcus neoformans ,Chromatography ,biology ,Chemistry ,Pentadecanoic acid ,Antimicrobial ,Mass spectrometry ,medicine.disease_cause ,biology.organism_classification ,High-performance liquid chromatography ,chemistry.chemical_compound ,Complementary and alternative medicine ,medicine ,Pharmacology (medical) ,Escherichia coli ,Two-dimensional nuclear magnetic resonance spectroscopy ,Quantitative analysis (chemistry) - Abstract
The chemical study of Cannabis sativa roots led to the isolation and identification of 10 compounds. Their chemical structures were unambiguously established on the basis of 1D and 2D NMR spectroscopy and mass spectrometry as friedelan-3-one (1), epifriedelanol (2), β-sitosterol (3), ergost-5-en-3-ol (4), methyl hexadecanoate (5), pentadecanoic acid (6), 10E-hexadecenoic acid (7), 4-hydroxy-3-methoxybenzaldehyde (8), β-sitosterol-β-D-glucoside (9) and p-coumaroyltyramine (10). Compounds 5–9 were reported for the first time from C. sativa roots. All the isolated compounds were tested for their antimicrobial activity. Compound 4 showed modest activity against Cryptococcus neoformans with an IC50 value of 13.7 μg/mL, while compound 10 displayed potent activity against Escherichia coli with an IC50 value of 0.8 μg/mL. A high-performance liquid chromatography method was developed and validated for the detection and quantification of p-coumaroyltyramine (10) in the extracts of different varieties of C. sativa roots.
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- 2018
13. Phytochemical and antimicrobial studies of Markhamia platycalyx (Baker) Sprague leaf
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Mohamed M. Radwan, Ehab M Mostafa, Basma K. Mahmoud, Mohamed Kamel, Mahmoud A. ElSohly, Mamdouh Nabil Samy, Amira S. Wanas, and Ashraf Hamed
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chemistry.chemical_compound ,Minimum inhibitory concentration ,Phytol ,Stigmasterol ,chemistry ,Phytochemical ,Candida glabrata ,biology ,Ursolic acid ,Traditional medicine ,Tormentic acid ,Antimicrobial ,biology.organism_classification - Abstract
Purpose: To isolate new antimicrobial agents from the leaves of Markhamia platycalyx (Baker) Sprague and assess their phytochemical characteristics and antimicrobial activity. Methods: Different chromatographic and spectroscopic techniques (NMR and ESI-MS) were applied for the identification of antimicrobial compounds. Agar-well diffusion technique was used for determination of antimicrobial activity. Anti-HCV effects were investigated using VITROS Anti-HCV assay. Results: Eighteen compounds were isolated for the first time from this genus. These were phytol, noctacosanoic acid (OCTA), tormentic acid and β-sitosterol-3-O-(6'-O-heptadecanoyl)-β-Dglucopyranoside. The other compounds were β-sitosterol, ursolic acid (URSA), oleanolic acids, pomolic acid (POMA), 2-epi-tormentic and β-sitosterol-3-O-β-D-glucopyranoside. However, stigmasterol and acteoside, which were seen in previous studies, were also present. Total ethanol extract (TEE) was the most effective against Escherichia coli, with the lowest minimum inhibitory concentration (MIC) of 1.0 μg/mL. Acteoside, URSA and 2-epi-tormentic acid showed the highest antibacterial effect on Pseudomonas aeruginosa while 2-epi-tormentic acid and acteoside produced the least MIC on Candida glabrata. These effects were superior to those produced by standard antibiotics. However, 2-epitormentic acid and β-sitosterol-3-O-β-D-glucopyranoside had no anti-HCV effects. Conclusion: Due to the good antimicrobial properties of Markhamia platycalyx, it is a potential source of new antimicrobial drugs.
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- 2021
14. Antimicrobial and GC/MS Studies for Saponifiable Matter and Volatile Oil of Markhamia platycalyx Leaves
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Mamdouh Nabil Samy, Basma K. Mahmoud, Mohamed Kamel, Amira S. Wanas, and Ashraf Hamed
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biology ,Traditional medicine ,Chemistry ,Markhamia ,Bignoniaceae ,Antimicrobial ,biology.organism_classification ,Bignonia ,Amikacin ,medicine ,Composition (visual arts) ,Gas chromatography–mass spectrometry ,Literature survey ,medicine.drug - Abstract
Bignoniaceae Juss. (Bignonia family) includes many genera of high economic and medicinal values. It comprises 104 genera and 860 species. Markhamia genus is traditionally used in the treatment of several diseases. Literature survey showed few chemical works on the Markhamia platycalyx and nothing could be found about the antimicrobial study. This provoked us to carry out an extensive study of this plant including GC/MS for saponifiable matter and volatile oil. The main recognized fatty acids were linolenic (44.66%), followed by palmitic (30.63%) of the saponifiable matter composition. While, E-phytol was the major identified compound of the volatile oil composition (12.95%). Moreover, this study included the antimicrobial activity of the volatile oil. It exhibited a potent antibacterial effect against E. coli with MIC 7.48 μg/ml compared to the standard antibiotics MICs, amikacin (28.96 μg/ml) and gentamycin (26.44 μg/ml).
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- 2021
15. In vivo anti-inflammatory and hepatoprotective activities of
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Safwat M S, Abo-Qotb, Ahmed M M, Hassanein, Samar Y, Desoukey, Amira S, Wanas, Heba M, Tawfik, and Mohammed A A, Orabi
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Mice ,Orobanche ,Plant Extracts ,Anti-Inflammatory Agents ,Animals ,Edema ,Plant Components, Aerial ,Antioxidants ,Rats - Abstract
A total methanolic extract and its sub-extracts of
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- 2020
16. Phytochemical and pharmacological appraisal of the aerial parts of
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Rokia M, Abdallah, Hala M, Hammoda, Mohamed M, Radwan, Nahla S, El-Gazzar, Amira S, Wanas, Mahmoud A, ElSohly, Maha A, El-Demellawy, Nevin M, Abdel-Rahman, and Shaimaa M, Sallam
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Plant Extracts ,Phytochemicals ,Lotus ,Egypt ,Plant Components, Aerial ,Saponins - Published
- 2020
17. Design, synthesis and antitrypanosomal activity of heteroaryl-based 1,2,4-triazole and 1,3,4-oxadiazole derivatives
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Mohamed Abdel-Aziz, Mohamed M. Radwan, Amira S. Wanas, Montaser Sh. A. Shaykoon, Ahmed M. Gouda, Osama M. Soltan, Adel A. Marzouk, and Bahaa G.M. Youssif
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Stereochemistry ,Trypanosoma brucei brucei ,Antiprotozoal Agents ,Protozoan Proteins ,Trypanosoma brucei ,01 natural sciences ,Biochemistry ,chemistry.chemical_compound ,Sterol 14-Demethylase ,Structure-Activity Relationship ,Drug Discovery ,medicine ,Molecular Biology ,IC50 ,chemistry.chemical_classification ,Oxadiazoles ,Binding Sites ,biology ,010405 organic chemistry ,Chemistry ,Organic Chemistry ,1,2,4-Triazole ,Triazoles ,biology.organism_classification ,In vitro ,0104 chemical sciences ,Molecular Docking Simulation ,010404 medicinal & biomolecular chemistry ,Enzyme ,Mechanism of action ,Docking (molecular) ,Drug Design ,Proton NMR ,medicine.symptom - Abstract
Two series of novel 1,2,4-triazol-3-yl-thioacetamide 3a-b and 4a-b and 5-pyrazin-2-yl-3H-[1,3,4]oxadiazole-2-thiones 9a-h were designed and synthesized. The compounds prepared have been identified using 1H NMR, 13C NMR and elemental analyses. The synthesized compounds 3a, 3b, 4a, 4b, 9a, 9b, 9d-e and 9f have been evaluated with α-difluoromethylornithine (DFMO) as a control drug for their in vitro antitrypanosomal activity against Trypanosoma brucei. Results showed that 3b was the most active compound in general and also more potent than control DFMO. 3b was 8 folds more potent than the reference with IC50 of 0.79 μM and IC90 of 1.35 μM, respectively compared to DFMO (IC50 = 6.10 μM and IC90 of 8.66 μM). The tested compounds showed moderate cytotoxicity with selectivity indices ranging from 12 (9d) to 102 (3b) against L6 cells. Docking study was performed into ten of T. brucei enzymes which have been identified as potential/valid targets for most of the antitrypanosomal agents. The results of the docking study revealed high binding scores toward many of the selected enzymes. A good correlation was observed only between log (IC50) of antitrypanosomal activity of the new compounds and their calculated Ki values against TryR enzyme (R2 = 0.726). Compound 3b, the most active as antitrypanosomal agents exhibited similar binding orientation and interaction to those of WP6 against TryR enzyme. However, in a next round of work, a complementary studies will be carried out to clarify the mechanism of action of these compounds.
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- 2020
18. Bioactivity-Guided Isolation of Potential Antidiabetic and Antihyperlipidemic Compounds from Trigonella stellata
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Mohamed M. Radwan, Michael L. Klein, Shabana I. Khan, Amira S. Wanas, Abdel-Azim M. Habib, Fahima F. Kassem, Safa M. Shams Eldin, Khaled M. Elokely, Mahmoud A. ElSohly, and Hala M. Hammoda
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0301 basic medicine ,Circular dichroism ,Soyasaponin I ,Magnetic Resonance Spectroscopy ,Stereochemistry ,Pharmaceutical Science ,01 natural sciences ,Analytical Chemistry ,03 medical and health sciences ,Cell Line, Tumor ,Drug Discovery ,Humans ,Hypoglycemic Agents ,Hypolipidemic Agents ,Pharmacology ,Plant Extracts ,010405 organic chemistry ,Chemistry ,Organic Chemistry ,Hep G2 Cells ,In vitro ,0104 chemical sciences ,Trigonella ,030104 developmental biology ,Complementary and alternative medicine ,Trigonella stellata ,Molecular Medicine ,Quercetin ,Two-dimensional nuclear magnetic resonance spectroscopy - Abstract
The in vitro antidiabetic and antihyperlipidemic activities of an alcoholic extract of Trigonella stellata were evaluated in terms of the activation of PPARα and PPARγ in human hepatoma (HepG2) cells. The extract was investigated phytochemically, aiming at the isolation of the most active compounds to be used as a platform for drug discovery. Three new isoflavans, (3S,4R)-4,2′,4′-trihydroxy)-7-methoxyisoflavan (1), (3R,4S)-4,2′,4′-trihydroxy-7-methoxy-4′-O-β-d-glucopyranosylisoflavan (2), and (2S,3R,4R)-4,2′,4′-trihydroxy-2,7-dimethoxyisoflavan (3), were isolated and characterized along with the five known compounds p-hydroxybenzoic acid (4), 7,4′-dihydroxyflavone (5), dihydromelilotoside (6), quercetin-3,7-O-α-l-dirhamnoside (7), and soyasaponin I (8). The structures of 1–3 were elucidated using various spectroscopic techniques including HRESIMS and 1D and 2D NMR. The absolute stereochemistry of the new isoflavans (1–3) was determined using both experimental and calculated electronic circular dichroism a...
- Published
- 2018
19. Monanchoramides A–D, ceramides from the marine sponge Monanchora clathrata with cytotoxic activity
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Mohamed A. Zaki, Amira S. Wanas, Thomas W. Shier, Mohamed Abou-Karam, Ali E. Raslan, Safwat A. Ahmed, Mohamed M. Radwan, Mahmoud A. ElSohly, Hashim A. Hassanean, and Alaa M. Nafady
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0301 basic medicine ,Ceramide ,biology ,medicine.drug_class ,Stereochemistry ,Uracil ,Plant Science ,biology.organism_classification ,Antimicrobial ,Biochemistry ,03 medical and health sciences ,chemistry.chemical_compound ,Sponge ,030104 developmental biology ,chemistry ,Acetylation ,Antiprotozoal ,medicine ,Cytotoxicity ,Agronomy and Crop Science ,Two-dimensional nuclear magnetic resonance spectroscopy ,Biotechnology - Abstract
Four new ceramides (1–4) were isolated from the sponge Monanchora clathrata, along with four epidioxysterols (5–8), two sterols (9, 10), uracil (11), and three triglycerides (12–14). All compounds were isolated for the first time from the genus Monanchora, also this is the first time for isolation and identification of compounds 5–8, and the ceramide moieties from the family Crambeidae. Acetylation of compounds (5–8) yielded two new chemically modified compounds (15, 16), in addition to the known 17, 18. Their chemical structures were elucidated using a combination of spectroscopic methods, including extensive 1D and 2D NMR, IR, HRESIMS, and GC/MS. The configuration of compounds 1–4 were assigned as 2S,3S,4R,2′R based on the modified Mosher’s reaction, optical rotation measurements and spectroscopic data comparison. The compounds were evaluated for their, cytotoxic, antiprotozoal, antimicrobial, and antimalarial potentials. Compound 1 showed remarkable cytotoxicity against MES-SA, MCF-7, and HK-2 cell lines with IC50 values of 3.29, 17.95 and 4.45 μM, respectively.
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- 2018
20. Quantitative Determination of Δ9-THC, CBG, CBD, Their Acid Precursors and Five Other Neutral Cannabinoids by UHPLC-UV-MS
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Mahmoud A. ElSohly, Bharathi Avula, Zlatko Mehmedic, Amira S. Wanas, Mohamed M. Radwan, Yan-Hong Wang, Ikhlas A. Khan, and Mei Wang
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Cannabigerol ,Formic acid ,Cannabaceae ,Pharmaceutical Science ,01 natural sciences ,High-performance liquid chromatography ,Gas Chromatography-Mass Spectrometry ,Cannabicyclol ,Analytical Chemistry ,03 medical and health sciences ,Cannabichromene ,chemistry.chemical_compound ,0302 clinical medicine ,Drug Discovery ,medicine ,Cannabidiol ,Dronabinol ,030216 legal & forensic medicine ,Chromatography, High Pressure Liquid ,Pharmacology ,Chromatography ,biology ,Cannabinoids ,010401 analytical chemistry ,Organic Chemistry ,biology.organism_classification ,0104 chemical sciences ,Complementary and alternative medicine ,chemistry ,Tetrahydrocannabinolic acid ,Cannabinol ,Molecular Medicine ,medicine.drug - Abstract
Cannabinoids are a group of terpenophenolic compounds in the medicinal plant Cannabis sativa (Cannabaceae family). Cannabigerolic acid, Δ9-tetrahydrocannabinolic acid A, cannabidiolic acid, Δ9-tetrahydrocannabinol, cannabigerol, cannabidiol, cannabichromene, and tetrahydrocannabivarin are major metabolites in the classification of different strains of C. sativa. Degradation or artifact cannabinoids cannabinol, cannabicyclol, and Δ8-tetrahydrocannabinol are formed under the influence of heat and light during processing and storage of the plant sample. An ultrahigh-performance liquid chromatographic method coupled with photodiode array and single quadruple mass spectrometry detectors was developed and validated for quantitative determination of 11 cannabinoids in different C. sativa samples. Compounds 1 – 11 were baseline separated with an acetonitrile (with 0.05% formic acid) and water (with 0.05% formic acid) gradient at a flow rate of 0.25 mL/min on a Waters Cortec UPLC C18 column (100 mm × 2.1 mm I. D., 1.6 µm). The limits of detection and limits of quantitation of the 11 cannabinoids were below 0.2 and 0.5 µg/mL, respectively. The relative standard deviation for the precision test was below 2.4%. A mixture of acetonitrile and methanol (80 : 20, v/v) was proven to be the best solvent system for the sample preparation. The recovery of all analytes was in the range of 97 – 105%. A total of 32 Cannabis samples including hashish, leaves, and flower buds were analyzed.
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- 2017
21. Two new iridoid glycosides from Odontonema cuspidatum and their bioactivities
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Mohamed S. Refaey, Ahmed Ali, Amira S. Wanas, Mahmoud A.H. Mostafa, and Ahmed M.M. Hassanein
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Iridoid Glycosides ,Cefotaxime ,biology ,010405 organic chemistry ,Stereochemistry ,Odontonema ,Acanthaceae ,Plant Science ,biology.organism_classification ,Antimicrobial ,01 natural sciences ,Biochemistry ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,chemistry.chemical_compound ,Activity measurements ,Verbascoside ,chemistry ,Glucoside ,medicine ,Agronomy and Crop Science ,Biotechnology ,medicine.drug - Abstract
Two new iridoid glycosides, named 6 β - O -methyl-unedoside ( 1 ) and 6 β - O -methyl-5-deoxythunbergioside ( 2 ) along with two known phenyl propanoid glucoside compounds dolichandroside A ( 3 ) and verbascoside ( 4 ) were isolated from the aerial parts of Odontonema cuspidatum (Nees) Kuntze (Acanthaceae). Structure elucidation of the new compounds was established by interpretation of their 1D and 2D-NMR spectral data by comparing with literatures, HRESIMS and optical activity measurement. The isolated compounds were tested for their in vitro antimicrobial and antimalarial activities. Compound 4 showed a moderate antimicrobial activity against E. coli with IC 50 value of 17.5 μM in comparing with Vancomycin and Cefotaxime.
- Published
- 2017
22. Cytotoxic activity evaluation and molecular docking study of phenolic derivatives from Achillea fragrantissima (Forssk.) growing in Egypt
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Basma M. Awad, Eman S. Habib, Mohamed A. Helal, Amany K. Ibrahim, Amira S. Wanas, Mohamed M. Radwan, Mahmoud A. ElSohly, and Safwat A. Ahmed
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chemistry.chemical_classification ,biology ,010405 organic chemistry ,Stereochemistry ,Eupatilin ,Organic Chemistry ,Flavonoid ,Ether ,Asteraceae ,biology.organism_classification ,01 natural sciences ,0104 chemical sciences ,HeLa ,010404 medicinal & biomolecular chemistry ,chemistry.chemical_compound ,chemistry ,Phytochemical ,Docking (molecular) ,medicine ,General Pharmacology, Toxicology and Pharmaceutics ,Cytotoxicity ,medicine.drug - Abstract
Achillea fragrantissima (Forssk) Sch. Bip. (Asteraceae) is one of the most important medicinal plant species in Egypt. Phytochemical investigation of the methanolic extract of A. fragrantissima led to the isolation of two phenolic compounds Piceol (1) and Veratric acid (2), which are reported for the first time from this plant. In addition, four known flavonoid compounds; Eupatilin 7-methyl ether (3), Chrysosplenol D (4), Cirsiliol (5), and Cirsimaritin (6) were isolated. Structure elucidation was achieved using spectroscopic techniques, including one-dimensional and two-dimensional nuclear magnetic resonance. Potential cytotoxic activities of the isolated compounds were measured by the sulphorhodamine B assay. Compounds 1, 2, 3, 4, 5, and 6 displayed cytotoxic activity against (MCF7) with IC50 values of 18.2, 15.7, 9.5, 8.33, 3.23, and 3.83 µg/ml, respectively, and against (HepG2) with IC50 values of 19.4, 41.2, 28.3, 20.8, 23.8, and 23.8 µg/ml, respectively. Also there was moderate cytotoxic activity against (A549) with IC50 values of 17.8, 13.6, 3.98, and 10.3 µg/ml for the compounds 1, 2, 4 and 5, respectively, and against (HeLa) with IC50 values of 10.1, 10.4, 4.88, and 3.98 µg/ml for the compounds 3, 4, 5, and 6, respectively. In addition, compounds 4 and 5 showed cytotoxic activity against (PC3) with IC50 values of 3.83 and 3.98 µg/ml, respectively. An in silico study was performed, where cirsiliol (5) and piceol (1) were docked into the active sites of the crystal structures of PI3K and Akt, two protein kinases which are involved in prostate and breast cancer proliferation and survival.
- Published
- 2017
23. New α-Pyrone derivatives from the endophytic fungus Embellisia sp
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Mahmoud A. ElSohly, Samir A. Ross, Ahmed M. Metwaly, Amira S. Wanas, and Mohamed M. Radwan
- Subjects
Antifungal ,010405 organic chemistry ,medicine.drug_class ,Chemistry ,Stereochemistry ,Organic Chemistry ,Pharmacology toxicology ,Absolute configuration ,Nuclear magnetic resonance spectroscopy ,Endophytic fungus ,01 natural sciences ,Embellisia sp ,Pyrone ,Plant use of endophytic fungi in defense ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,chemistry.chemical_compound ,medicine ,Organic chemistry ,General Pharmacology, Toxicology and Pharmaceutics - Abstract
Two new α- Pyrone derivatives (1,2) have been isolated from the endophytic fungus Embellisia sp. The isolated compounds were chemically identified as 5-(3-S-hydroxybutyl)-4-methoxy-6-methyl-2H-pyran-2-one (1) and 4-(4-methoxy-6-methyl-2-oxo-2H-pyran-5-yl)butanoic acid (2). The chemical structures of 1 and 2 were determined based on one and two dimensional NMR spectroscopy and high resolution mass spectrometry. Absolute configuration of compound 1 was determined using modified mosher ester reaction. In vitro antileishmanial, antifungal, antibacterial and antimalarial activities of 1 and 2 were examined but they didn’t show promising activities.
- Published
- 2017
24. Effect of tobacco tar on Staphylococcus aureus and Candida albicans biofilm formation
- Author
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Amany Hamouda Mahmoud, Rehab Mahmoud Abd El-Baky, Mamdouh Nabil Samy, Mohamed Kamel, Usama Ramdan Abdel-Mohsen, and Amira S. Wanas
- Subjects
0301 basic medicine ,biology ,Chemistry ,030106 microbiology ,Biofilm ,Plant Science ,biology.organism_classification ,Antimicrobial ,medicine.disease_cause ,Microbiology ,Corpus albicans ,Penicillin ,03 medical and health sciences ,Minimum inhibitory concentration ,030104 developmental biology ,Infectious Diseases ,Tar (tobacco residue) ,Staphylococcus aureus ,medicine ,Candida albicans ,medicine.drug - Abstract
Twenty compounds were determined in the tar of cigarette smoke. The tar was obtained using tobacco filters and then analyzed by liquid chromatography-mass spectrometry (LC-MS). Tobacco tar expressed marked decrease in the susceptibility of Staphylococcus aureus to penicillin, tetracycline and amoxicillin/clavulanic antibiotics and showed marked decrease in susceptibility of Candida albicans to the tested antifungals except for clotrimazole and ketoconazole. Tobacco tar decreased the permeability of the tested organisms to ethidium bromide in the presence of antimicrobials, after 2 h for both S. aureus (17.8-20%) and C. albicans (13.3-16.3%) and decreased the adherence of the tested microorganisms at concentrations above Minimal Inhibitory Concentration (MIC). Tobacco tar showed a marked increase in the hydrophobicity of the tested microorganisms by 2.5 to 7 fold. Tobacco tar increased or upregulated ALS1 and HWP1 genes that play an important role in adhesion, hyphae formation and biofilm formation of C. albicans and increased the expression of ica A gene that regulate biofilm formation of S. aureus. Key words: Tobacco tar, Staphylococcus aureus, Candida albicans, antimicrobial susceptibility, ethidium bromide, biofilm formation.
- Published
- 2017
25. Repellent Activity of Carrot Seed Essential Oil and Its Pure Compound, Carotol, Against Mosquitoes
- Author
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Mohamed M. Radwan, Abbas Ali, Ikhlas A. Khan, and Amira S. Wanas
- Subjects
0106 biological sciences ,Mosquito Control ,030231 tropical medicine ,Fractionation ,Aedes aegypti ,01 natural sciences ,law.invention ,Carotol ,DEET ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,law ,Aedes ,Anopheles ,Oils, Volatile ,Bioassay ,Flame ionization detector ,Animals ,Food science ,Ecology, Evolution, Behavior and Systematics ,Essential oil ,biology ,fungi ,Public Health, Environmental and Occupational Health ,General Medicine ,biology.organism_classification ,Daucus carota ,010602 entomology ,chemistry ,Insect Science ,Insect Repellents ,Seeds ,Gas chromatography - Abstract
In our natural products screening program for mosquitoes, carrot seed essential oil showed high repellency. The gas chromatography (GC)/flame ionization detector and GC/mass spectrometry analysis of the essential oil revealed the presence of 47 compounds. Carotol was more than 75% w/w, followed by muurolene (4.86%), (Z)-β-farnesene (2.9%), and diepicedrene (1.1%). Systematic bioassay-guided fractionation of the essential oil was performed to identify active repellent compounds. In both Klun and Debboun (K&D) and Ali and Khan (A&K) bioassays, carotol showed biting deterrent activity similar to N,N-Diethyl-3-methylbenzamide (deet) and carrot seed essential oil against both Aedes aegypti and Anopheles quadrimaculatus, while in in vivo cloth patch bioassay, the minimum effective dose (MED) of deet was lower (12.5 μg/cm2) than the essential oil and carotol (25 μg/cm2) against Ae. aegypti. In the A&K bioassay, the MED values were similar, whereas the values of the mixtures of deet with essential oil and carotol was lower (6.25 + 6.25 = 12.5 μg/cm2) than their individual treatments (25 μg/cm2). In direct skin application bioassay, both the essential oil and carotol provided good repellency. The mixtures of deet and essential oil or carotol significantly increased the residual activity, indicating synergism. Mosquito repellency of the essential oil and carotol is reported for the 1st time. These data indicate the potential of these natural products to be developed as commercial repellents.
- Published
- 2019
26. Analysis of Terpenes in Cannabis sativa L. Using GC/MS: Method Development, Validation, and Application
- Author
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Baharthi Avula, Hemant Lata, Amira S. Wanas, Mahmoud A. ElSohly, Suman Chandra, Yan-Hong Wang, Ikhlas A. Khan, Ghada M. Hadad, Elsayed A. Ibrahim, Amany K. Ibrahim, Mei Wang, Chandrani G. Majumdar, Mohamed M. Radwan, Randa A. Abdel Salam, and Safwat A. Ahmed
- Subjects
Pharmacology ,Detection limit ,Limonene ,Chromatography ,biology ,Terpenes ,Organic Chemistry ,Cannabaceae ,Ethyl acetate ,Pharmaceutical Science ,Reproducibility of Results ,Repeatability ,biology.organism_classification ,Gas Chromatography-Mass Spectrometry ,Analytical Chemistry ,Terpene ,chemistry.chemical_compound ,Complementary and alternative medicine ,Linalool ,chemistry ,Limit of Detection ,Drug Discovery ,Molecular Medicine ,Gas chromatography–mass spectrometry ,Cannabis - Abstract
Terpenes are the major components of the essential oils present in various Cannabis sativa L. varieties. These compounds are responsible for the distinctive aromas and flavors. Besides the quantification of the cannabinoids, determination of the terpenes in C. sativa strains could be of importance for the plant selection process. At the University of Mississippi, a GC-MS method has been developed and validated for the quantification of terpenes in cannabis plant material, viz., α-pinene, β-pinene, β-myrcene, limonene, terpinolene, linalool, α-terpineol, β-caryophyllene, α-humulene, and caryophyllene oxide. The method was optimized and fully validated according to AOAC (Association of Official Analytical Chemists) guidelines against reference standards of selected terpenes. Samples were prepared by extraction of the plant material with ethyl acetate containing n-tridecane solution (100 µg/mL) as the internal standard. The concentration-response relationship for all analyzed terpenes using the developed method was linear with r2 values > 0.99. The average recoveries for all terpenes in spiked indoor cultivated samples were between 95.0 – 105.7%, with the exception of terpinolene (67 – 70%). The measured repeatability and intermediate precisions (% relative standard deviation) in all varieties ranged from 0.32 to 8.47%. The limit of detection and limit of quantitation for all targeted terpenes were determined to be 0.25 and 0.75 µg/mL, respectively. The proposed method is highly selective, reliable, and accurate and has been applied to the simultaneous determination of these major terpenes in the C. sativa biomass produced by our facility at the University of Mississippi as well as in confiscated marijuana samples.
- Published
- 2019
27. Chemical constituents, Antibacterial and Acetylcholine esterase inhibitory activity of
- Author
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Fathalla M, Harraz, Hala M, Hammoda, Amr, El-Hawiet, Mohamed M, Radwan, Amira S, Wanas, Asmaa M, Eid, and Mahmoud A, ElSohly
- Subjects
Methicillin-Resistant Staphylococcus aureus ,Plant Leaves ,Plant Extracts ,Cholinesterase Inhibitors ,Glycosides ,Cupressus ,Lignans ,Anti-Bacterial Agents - Abstract
The chemical constituents of
- Published
- 2018
28. Design and synthesis of new 1,6-dihydropyrimidin-2-thio derivatives targeting VEGFR-2: Molecular docking and antiproliferative evaluation
- Author
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Salah A. Abdel-Aziz, Kamal Abdelrahman, Bahaa G.M. Youssif, Ahmed M. Gouda, Amira S. Wanas, Adel A. Marzouk, and Mohamed Abdel-Aziz
- Subjects
Sorafenib ,Programmed cell death ,Stereochemistry ,Thio ,Antineoplastic Agents ,Apoptosis ,01 natural sciences ,Biochemistry ,Structure-Activity Relationship ,Cell Line, Tumor ,Drug Discovery ,medicine ,Humans ,Sulfhydryl Compounds ,Protein Kinase Inhibitors ,Molecular Biology ,Cell Proliferation ,Dose-Response Relationship, Drug ,Molecular Structure ,biology ,010405 organic chemistry ,Chemistry ,Melanoma ,Organic Chemistry ,Active site ,medicine.disease ,Vascular Endothelial Growth Factor Receptor-2 ,In vitro ,0104 chemical sciences ,Molecular Docking Simulation ,010404 medicinal & biomolecular chemistry ,Pyrimidines ,Docking (molecular) ,Cell culture ,Drug Design ,biology.protein ,Drug Screening Assays, Antitumor ,medicine.drug - Abstract
A series of new 1,6-dihydropyrimidin-2-thiol derivatives (scaffold A) as VEGFR-2 inhibitors has been designed and synthesized. Compounds 3a, 3b, 3e and 4b have been selected for in vitro anticancer screening by the National Cancer Institute. Compound 3e showed remarkable anticancer activity against most of the cell lines tested, where a complete cell death against leukemia, non-small cell lung cancer, colon, CNS, melanoma, and breast cancer cell lines was observed. In vitro five dose tests showed that compound 3e had high activity against most of the tested cell lines with GI50 ranging from 19 to 100 μM and selectivity ratios ranging between 0.75 and 1.71 at the GI50 level. VEGFR-2-kinase was tested against 3a, 3b, 3e, 4b and sorafenib was used as a reference. Compounds 3a and 3e were the most potent analogues with IC50 values of 386.4 nM and 198.7 nM against VEGFR-2, respectively, in comparison to sorafenib (IC50 = 0.17 nM). The results of the docking study showed a good fitting of the new compounds to the active site of VEGFR-2 with binding free energies in the range of −9.80 to −11.25 kcal/mol compared to −12.12 kcal/mol for sorafenib. Compounds 4a-e with the hydroxyimino group had a higher affinity to VEGFR-2 than their parent derivatives 3a-e.
- Published
- 2020
29. A Validated UPLC-PDA Method for Simultaneous Determination of 3 Biologically Active Isoflavans in Trigonella stellata Extract
- Author
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Amira S. Wanas, Abdel-Azim M. Habib, Fahima F. Kassem, Hala M. Hammoda, Safa M. Shams Eldin, Mohamed M. Radwan, and Mahmoud A. ElSohly
- Subjects
Pharmacology ,Chromatography ,010405 organic chemistry ,Chemistry ,Uplc pda ,010401 analytical chemistry ,Plant Science ,General Medicine ,01 natural sciences ,High-performance liquid chromatography ,Quantitative determination ,0104 chemical sciences ,chemistry.chemical_compound ,Complementary and alternative medicine ,Glucoside ,Trigonella stellata ,Drug Discovery - Abstract
In this study, an ultra-performance liquid chromatography (UPLC)/photodiode array method was developed for the simultaneous determination of trigonellan glucoside (1), isotrigonellan (2), and methoxy-isotrigonellan (3) in Trigonella stellata extract using an external standard method. The extract was prepared using a standardized method by maceration of the dried plant material in ethanol. The 3 isoflavans (1-3) were separated on an Acquity UPLC C18 column using gradient elution with a mobile phase consisting of 0.1% (v/v) formic acid aqueous solution and 0.1% (v/v) formic acid in acetonitrile, and ultraviolet detection. The method provides a linear correlation for all analytes over the investigated ranges with all correlation coefficients greater than 0.998. The validated lower limits of quantitation were 53, 127, and 5 μg/mL for isoflavans 1, 2, and 3, respectively. Intraday and interday precisions (percent relative SD [RSD%]) were less than 8.3% and accuracy (RE%) ranged from 90% to 100%. The method’s capability to remain unaffected by small, but deliberate variations in method parameters (method’s reliability during normal usage) described by the robustness showed RSD% less than 4.6% measured by varying 3 different parameters. The validated method was successfully applied to simultaneously determine the concentration of the 3 new isoflavans having anti-inflammatory and antidiabetic activities. The results revealed that the validated method can be used for quality control of herbal preparations containing these or similar isoflavans that are marketed for the prevention of inflammation and as antidiabetics.
- Published
- 2020
30. Chemical Composition of Volatile Oils of Fresh and Air-Dried Buds of Cannabis chemovars, Their Insecticidal and Repellent Activities
- Author
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Abbas Ali, Suman Chandra, Mahmoud A. ElSohly, Betül Demirci, Khc Başer, Hemant Lata, Amira S. Wanas, Zlatko Mehmedic, Mohamed M. Radwan, and Anadolu Üniversitesi
- Subjects
gas chromatography ,Plant Science ,03 medical and health sciences ,0302 clinical medicine ,Drug Discovery ,medicine ,Food science ,Tetrahydrocannabinol ,Chemical composition ,030304 developmental biology ,Pharmacology ,0303 health sciences ,biology ,Chemotype ,Chemistry ,General Medicine ,biology.organism_classification ,Complementary and alternative medicine ,cannabis essential oils ,larvicidal ,Cannabis ,Gas chromatography ,insecticidal ,Cannabidiol ,030217 neurology & neurosurgery ,medicine.drug - Abstract
WOS: 000534529000001, The volatile oils of fresh and air-dried buds of 3 different varieties of Cannabis, namely, high cannabidiol (CBD) chemotype, inter- mediate CBD/tetrahydrocannabinol chemotype, and high THC chemotype were prepared by hydrodistillation. Gas chro- matography analysis of the volatile oils resulted in the identification of 71 compounds, of which 33 were monoterpenes and 38 were sesquiterpenes. the volatile oil obtained from the THC chemotype showed an increase in the ratio of the sesquiterpenes to monoterpenes content. the content of terpinolene was dramatically decreased upon drying of THC chemotype. Moderate increase in beta-caryophyllene and caryophyllene oxide was observed. However, there was no detectable change in the percentage of monoterpenes and sesquiterpenes content in both the intermediate type and CBD chemotype upon drying. the insecticidal activity of the volatile oils was evaluated. the oil obtained from the fresh and dried high CBD cannabis showed good biting deterrent activity at 10 ug/cm(2) compared with N,N-diethyl-meta-toluamide at 4.78 mu g/cm(2), and good larvicidal activity., National Institute on Drug AbuseUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute on Drug Abuse (NIDA) [N01DA-15-7793], The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: this work was supported in part by the National Institute on Drug Abuse, contract #N01DA-15-7793.
- Published
- 2020
31. Polybrominated diphenyl ethers from the marine organisms Lendenfeldia dendyi and Sinularia dura with anti-MRSa activity
- Author
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Frank R. Fronczek, Melissa R. Jacob, Amira S. Wanas, Mohamed M. Radwan, and Samir A. Ross
- Subjects
Lendenfeldia ,biology ,Stereochemistry ,Chemistry ,Organic Chemistry ,Sinularia dura ,Anti mrsa ,Antimicrobial ,biology.organism_classification ,medicine.disease_cause ,Sponge ,Polybrominated diphenyl ethers ,Staphylococcus aureus ,medicine ,General Pharmacology, Toxicology and Pharmaceutics ,Sinularia - Abstract
Bioassay-guided fractionation of the marine sponge Lendenfeldia dendyi and the soft coral Sinularia dura resulted in the isolation of five polybrominated diphenyl ethers (1–5). The structures of the isolated compounds were determined using spectroscopic methods (1D and 2D NMR) and HRMS analyses. The absolute structures of compounds 1 and 2 were confirmed by X-ray analysis. Antimicrobial testing of the isolated compounds along with the acetylated derivative of compound 2 (2a) indicated that they displayed a significant and selective activity against methicillin-resistant Staphylococcus aureus (MRSa) with IC50 values of
- Published
- 2015
32. Structure elucidation of secondary metabolites isolated from the leaves of Ixora undulate and their inhibitory activity toward advanced glycation end-products formation
- Author
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Amira S. Wanas, Katsuyoshi Matsunami, Tsutomu Mizuta, Sachiko Sugimoto, Mohamed Kamel, and Hideaki Otsuka
- Subjects
Glycation End Products, Advanced ,Stereochemistry ,Flavonoid ,chemistry.chemical_element ,Ixora ,Rubiaceae ,Plant Science ,Horticulture ,Inhibitory postsynaptic potential ,Plant Roots ,Biochemistry ,Inhibitory Concentration 50 ,Glycation ,Organic chemistry ,Glycosides ,Nuclear Magnetic Resonance, Biomolecular ,Molecular Biology ,Flavonoids ,chemistry.chemical_classification ,Molecular Structure ,biology ,Chemistry ,Glycoside ,General Medicine ,biology.organism_classification ,Sulfur ,Plant Leaves ,Egypt ,Two-dimensional nuclear magnetic resonance spectroscopy - Abstract
Three aromatic glycosides (1-3), two sulfur and nitrogen-containing compound glucosides (4, 5), and one flavonoid glycoside (6) were isolated from the leaves of Ixora undulata. Their structures were established by extensive 1D, 2D NMR, and HRESIMS experiments, and structure 4 was further confirmed by single crystal X-ray diffraction analysis. Of the assayed compounds, 7, 11 and 12 showed strong inhibitory activity toward advanced glycation end-products formation with IC50 values of 86.0 μM, 76.6 μM and 98.6 μM, respectively.
- Published
- 2014
33. Analysis of Cupressuflavone and Amentoflavone from Cupressus sempervirens L. and its Tissue Cultured Callus using HPLC-DAD Method
- Author
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Ghada M. Hadad, Mahmoud A. ElSohly, Elsayed A. Ibrahim, Samar Y. Desoukey, Mohamed M. Radwan, Amira S. Wanas, Safwat A. Ahmed, a A Abdel Salam, and Amany K. Ibrahim
- Subjects
0106 biological sciences ,Chromatography ,biology ,010405 organic chemistry ,Cupressus ,Amentoflavone ,Pharmacognosy ,biology.organism_classification ,01 natural sciences ,0104 chemical sciences ,Cupressuflavone ,chemistry.chemical_compound ,chemistry ,Endocrine pharmacology ,Callus ,Cupressus sempervirens ,Hplc dad ,010606 plant biology & botany - Published
- 2017
34. Evaluation of Secondary Metabolites from the Red Sea Tunicate Polyclinum Constellatum
- Author
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Hashim A. Hassanean, Mahmoud A. ElSohly, Alaa M. Nafady, Mohamed M. Radwan, Ali E. Raslan, Babu L. Tekwani, Safwat A. Ahmed, and Amira S. Wanas
- Subjects
Polyclinum ,food.ingredient ,food ,Community pharmacy ,Endocrine pharmacology ,Zoology ,Cardiovascular pharmacology ,Pharmacology ,Biology ,biology.organism_classification ,Tunicate - Published
- 2017
35. Flavonoids of Alcea rosea L. and their immune stimulant, antioxidant and cytotoxic activities on hepatocellular carcinoma HepG-2 cell line
- Author
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Nevin M Abdel-Rahman, Sonia Piacente, Maha A El-Demellawy, Amira S. Wanas, Mahmoud A. ElSohly, Shaimaa M. Sallam, Mohamed M. Radwan, Nabila M Ghazy, Nabil A. Abdel-Salam, and Mary L. Shenouda
- Subjects
Antioxidant ,antioxidant ,medicine.drug_class ,medicine.medical_treatment ,Drug Evaluation, Preclinical ,Alcea roseaL ,cytotoxic ,flavonoids ,HepG-2 ,immunostimulant ,phytochemical ,Analytical Chemistry ,Biochemistry ,Plant Science ,Organic Chemistry ,Flowers ,01 natural sciences ,Immunostimulant ,Antioxidants ,Inhibitory Concentration 50 ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Immune system ,Adjuvants, Immunologic ,medicine ,Humans ,Cytotoxic T cell ,Apigenin ,Malvaceae ,Plants, Medicinal ,biology ,Traditional medicine ,Plant Extracts ,Hep G2 Cells ,biology.organism_classification ,Antineoplastic Agents, Phytogenic ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,chemistry ,Phytochemical ,Cell culture ,030220 oncology & carcinogenesis ,Egypt ,Alcea rosea - Abstract
Alcea rosea L. is widely cultivated in gardens of Egypt as an ornamental plant and it has a great history of folkloric medicinal uses. In the present work, phytochemical investigation of the alcoholic extract of the flowers of A. rosea L. led to the isolation of six flavonoids (1–6). Dihydrokaempferol-4′-O-β-d-glucopyranoside (1), dihydrokaempferol (2), kaempferol-3-O-[6″-(E-coumaroyl)]-β-d-glucopyranoside (3), kaempferol-3-O-β-d-glucopyranoside (4), Apigenin (5) and kaempferol-3-O-α-l-rhamnopyranosyl-(1′″→6″)-β-d-glucopyranoside (6). Four of the isolated compounds were evaluated for their antioxidant, immunostimulant and cytotoxic activities against HepG-2 cell line. Compound (3) showed potent cytotoxic activity against HepG-2 cell line with high selectivity towards hepatocellular carcinoma in vitro (with IC50 = 3.8 μg/mL). Compounds 1 and 2 exhibited significant antioxidant activity and compound 4 showed a significant immune stimulant activity. Compound 1 is isolated for the first time from genus Alcea and this is the first report for its biological investigation.
- Published
- 2017
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36. Natural Cannabinoids of Cannabis and Methods of Analysis
- Author
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Mahmoud A. ElSohly, Mohamed M. Radwan, Amira S. Wanas, and Suman Chandra
- Subjects
0106 biological sciences ,03 medical and health sciences ,0302 clinical medicine ,biology ,Traditional medicine ,Cannabis product ,Illicit drug ,Cannabis ,Cannabis sativa ,biology.organism_classification ,01 natural sciences ,030217 neurology & neurosurgery ,010606 plant biology & botany - Abstract
Cannabis has gained a lot of popularity in last few years not only because of its use as illicit drug but due to its use as food, fiber and medicine. It is a complex mixture of constituents which contain a unique class of secondary metabolites called phytocannabinoids. In general, so far a total of 565 constituents including 120 phytocannabinoids have been reported in Cannabis sativa. This chapter discusses the chemistry of phytocannabinoids in the plant with particular emphasis on the Δ9-THC type of cannabinoids and different analytical methods available for cannabinoids analysis in cannabis plant and cannabis products.
- Published
- 2017
37. Development And Validation Of An UHPSFC-DAD/MS Method For The Qualitative And Quantitative Determination Of Major Cannabinoids In Cannabis Plant Extracts And Products
- Author
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Mohamed M. Radwan, YH Wang, Bharathi Avula, Zlatko Mehmedic, Ikhlas A. Khan, MA ElSohly, Jon F. Parcher, Mei Wang, Amira S. Wanas, and J Van Antwerp
- Subjects
Pharmacology ,Chromatography ,Complementary and alternative medicine ,biology ,Chemistry ,Organic Chemistry ,Drug Discovery ,Pharmaceutical Science ,Molecular Medicine ,Cannabis ,biology.organism_classification ,Quantitative determination ,Analytical Chemistry - Published
- 2016
38. Phenolics And Isoflavans From Trigonella Stellata And Their NF-Kb And Inos Inhibitory Activity
- Author
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MA ElSohly, Hala M. Hammoda, Shabana I. Khan, Amira S. Wanas, Abdel-Azim M. Habib, Fahima F. Kassem, SM Shams Eldin, and Mohamed M. Radwan
- Subjects
Pharmacology ,Complementary and alternative medicine ,Traditional medicine ,Chemistry ,Trigonella stellata ,Organic Chemistry ,Drug Discovery ,Pharmaceutical Science ,Molecular Medicine ,Inhibitory postsynaptic potential ,Analytical Chemistry - Published
- 2016
39. An Investigation Of Decarboxylation Of Acidic Cannabinoids: THCA-A, CBDA And CBGA
- Author
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Jon F. Parcher, YH Wang, Ikhlas A. Khan, Bharathi Avula, Mei Wang, J Van Antwerp, Mohamed M. Radwan, MA ElSohly, and Amira S. Wanas
- Subjects
Pharmacology ,Complementary and alternative medicine ,Decarboxylation ,Chemistry ,Organic Chemistry ,Drug Discovery ,Pharmaceutical Science ,Molecular Medicine ,Organic chemistry ,Analytical Chemistry - Published
- 2016
40. Phytochemical Investigation Of Senecio Vulgaris L
- Author
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Amira S. Wanas, Hala M. Hammoda, Fahima F. Kassem, MA ElSohly, Mohamed M. Radwan, Aam Habib, and RS Darwish
- Subjects
Pharmacology ,Complementary and alternative medicine ,Phytochemical ,biology ,Organic Chemistry ,Drug Discovery ,Botany ,Senecio vulgaris ,Pharmaceutical Science ,Molecular Medicine ,biology.organism_classification ,Analytical Chemistry - Published
- 2016
41. Phytochemical Investigation Of Lotus Corniculatus Growing In Egypt
- Author
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Mohamed M. Radwan, MA ElSohly, Amira S. Wanas, RM Abdallah, Hala M. Hammoda, NS El-Gazza, and SM Sallam
- Subjects
Soyasaponin I ,Lotus ,Pharmaceutical Science ,01 natural sciences ,Analytical Chemistry ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Drug Discovery ,Botany ,Lotus corniculatus ,Afzelin ,Pharmacology ,biology ,Lotus lalambensis ,Traditional medicine ,010405 organic chemistry ,Chemistry ,Organic Chemistry ,biology.organism_classification ,Coumarin ,0104 chemical sciences ,Complementary and alternative medicine ,Phytochemical ,030220 oncology & carcinogenesis ,Molecular Medicine - Abstract
Extracts of aerial parts of Lotus corniculatus L. exhibited significant antioxidant, immunostimulant and anticancer activities [1]. Bioactivity-guided fractionation of the aerial parts of L. corniculatus led to the isolation of seven known compounds (1-7). The structure elucidation of the isolated compounds was achieved using spectroscopic techniques, including 1D and 2D NMR. Their chemical structures were identified as: 7,2'-dihydroxy-4'-methoxyisoflavan (vestitol) (1), kaempferol-3-O-rhamnoside (afzelin) (2), kaempferol-3,7-O-dirhamnoside (kaempferitin) (3), kaempferol-3-O-(2"-xylopyranosyl) galactopyranoside (4), soyasapogenol-β-3-O-glucopyranoside (5), soyasapogenol-β-3-O-[α-L-rhamnopyranosyl-(1'''→2'')-β-D-galactopyranosyl-(1''→2')-β-D-glucuronopyranoside] (soyasaponin I) (6) and methyl -O-β-D-glucopyranoside (7). Compounds 4 and 5 are reported for the first time from the genus Lotus. References: [1] Alqasoumi, S.I.; Al-Rehaily, A.J.; Abdel-Kader, M.S. "Two isoflavans and a 3-aryl coumarin from the roots of Lotus lalambensis growing in Saudi Arabia", Revistalatinoamericana de Quimica, 41 (1):61 (2013).
- Published
- 2016
42. Phytochemical And Biological Evaluation Of Alcea Rosea L., Growing In Egypt
- Author
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NA Ghazy, SM Sallam, Mohamed M. Radwan, MA ElSohly, NM Abd El-Salam, ML Shenouda, Amira S. Wanas, and Sonia Piacente
- Subjects
Pharmacology ,biology ,Traditional medicine ,Organic Chemistry ,Pharmaceutical Science ,biology.organism_classification ,Analytical Chemistry ,Complementary and alternative medicine ,Phytochemical ,Drug Discovery ,Botany ,Molecular Medicine ,Alcea rosea ,Biological evaluation - Published
- 2016
43. Quantitative Determination of Cannabinoids in Cannabis and Cannabis Products Using Ultra-High-Performance Supercritical Fluid Chromatography and Diode Array/Mass Spectrometric Detection
- Author
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Mahmoud A. ElSohly, Amira S. Wanas, Bharathi Avula, Mei Wang, John van Antwerp, Ikhlas A. Khan, Yan-Hong Wang, Zlatko Mehmedic, and Mohamed M. Radwan
- Subjects
Analytical chemistry ,Mass spectrometry ,01 natural sciences ,Mass Spectrometry ,Pathology and Forensic Medicine ,chemistry.chemical_compound ,Limit of Detection ,Genetics ,Humans ,Least-Squares Analysis ,Derivatization ,Cannabis ,Principal Component Analysis ,Chromatography ,biology ,Molecular Structure ,010405 organic chemistry ,Elution ,Cannabinoids ,Plant Extracts ,010401 analytical chemistry ,Analytical technique ,Chromatography, Supercritical Fluid ,biology.organism_classification ,Quantitative determination ,0104 chemical sciences ,chemistry ,Supercritical fluid chromatography ,Ultra high performance - Abstract
Ultra-high-performance supercritical fluid chromatography (UHPSFC) is an efficient analytical technique and has not been fully employed for the analysis of cannabis. Here, a novel method was developed for the analysis of 30 cannabis plant extracts and preparations using UHPSFC/PDA-MS. Nine of the most abundant cannabinoids, viz. CBD, ∆8 -THC, THCV, ∆9 -THC, CBN, CBG, THCA-A, CBDA, and CBGA, were quantitatively determined (RSDs < 6.9%). Unlike GC methods, no derivatization or decarboxylation was required prior to UHPSFC analysis. The UHPSFC chromatographic separation of cannabinoids displayed an inverse elution order compared to UHPLC. Combining with PDA-MS, this orthogonality is valuable for discrimination of cannabinoids in complex matrices. The developed method was validated, and the quantification results were compared with a standard UHPLC method. The RSDs of these two methods were within ±13.0%. Finally, chemometric analysis including principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) were used to differentiate between cannabis samples.
- Published
- 2016
44. Determination of 11 Cannabinoids in Biomass and Extracts of Different Varieties of Cannabis Using High-Performance Liquid Chromatography
- Author
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Shahbaz W. Gul, Amira S. Wanas, Waseem Gul, Maged H. M. Sharaf, Zlatko Mehmedic, Mohamed M. Radwan, Mahmoud A. ElSohly, and Ikhlas I Khan
- Subjects
Pharmacology ,Chromatography ,Cannabigerol ,Cannabinoids ,Plant Extracts ,Extraction (chemistry) ,Tetrahydrocannabivarin ,High-performance liquid chromatography ,Cannabicyclol ,Analytical Chemistry ,chemistry.chemical_compound ,Cannabichromene ,chemistry ,medicine ,Cannabinol ,Environmental Chemistry ,Biomass ,Agronomy and Crop Science ,Cannabidiol ,Chromatography, High Pressure Liquid ,Food Science ,medicine.drug ,Cannabis - Abstract
An HPLC single-laboratory validation was performed for the detection and quantification of the 11 major cannabinoids in most cannabis varieties, namely, cannabidiolic acid (CBDA), cannabigerolic acid (CBGA), cannabigerol (CBG), cannabidiol (CBD), tetrahydrocannabivarin (THCV), cannabinol (CBN), Δ9-trans-tetrahydrocannabinol (Δ9-THC), Δ8- trans-tetrahydrocannabinol (Δ8-THC), cannabicyclol (CBL), cannabichromene (CBC), and Δ9-tetrahydrocannabinolic acid-A (THCAA). The analysis was carried out on the biomass and extracts of these varieties. Methanol–chloroform (9:1, v/v) was used for extraction, 4-androstene-3,17-dione was used as the internal standard, and separation was achieved in 22.2 min on a C18 column using a two- step gradient elution. The method was validated for the 11 cannabinoids. The concentration-response relationship of the method indicated a linear relationship between the concentration and peak area with r2 values of >0.99 for all 11 cannabinoids. Method accuracy was determined through a spike study, and recovery ranged from 89.7 to 105.5% with an RSD of 0.19 to 6.32% for CBDA, CBD, THCV, CBN, Δ9-THC, CBL, CBC, and THCAA; recovery was 84.7, 84.2, and 67.7% for the minor constituents, CBGA, CBG, and Δ8-THC, respectively, with an RSD of 2.58 to 4.96%. The validated method is simple, sensitive, and reproducible and is therefore suitable for the detection and quantification of these cannabinoids in different types of cannabis plant materials.
- Published
- 2015
45. Triterpene Glycosides and Glucosyl Esters, and a Triterpene from the Leaves of Schefflera actinophylla
- Author
-
Samar Y. Desoukey, Katsuyoshi Matsunami, Mostafa A. Fouad, Amira S. Wanas, Hideaki Otsuka, and Mohamed Kamel
- Subjects
chemistry.chemical_classification ,Schefflera actinophylla ,Magnetic Resonance Spectroscopy ,biology ,Chemistry ,Chemical structure ,Molecular Conformation ,Glycoside ,Esters ,General Chemistry ,General Medicine ,biology.organism_classification ,Triterpenes ,Plant Leaves ,Terpene ,chemistry.chemical_compound ,Triterpene ,Drug Discovery ,Botany ,Araliaceae ,Glycosyl ,Glycosides ,Chemical composition - Abstract
From the EtOAc and 1-BuOH fractions, three new ursane-type and four new lupane-type triterpenes, along with nine known glycosides and glycosyl esters of lupane-type were isolated from the leaves of Schefflera actinophylla. All the isolated compounds were obtained for the first time from this plant. The structures of the new triterpenes were determined through a combination of spectroscopic and chemical analyses.
- Published
- 2010
46. Anticancer metabolites from Achillea fragrantissima
- Author
-
MA ElSohaly, Amira S. Wanas, E Sand, B Awad, Amany K. Ibrahim, Safwat A. Ahmed, and Mohamed M. Radwan
- Subjects
Pharmacology ,Complementary and alternative medicine ,Traditional medicine ,business.industry ,Organic Chemistry ,Drug Discovery ,Pharmaceutical Science ,Molecular Medicine ,Medicine ,Achillea fragrantissima ,business ,Analytical Chemistry ,Biotechnology - Published
- 2015
47. UHPLC-UV/MS fingerprint method for quantitative determination of THC, CBD, CBG, and other eight cannabinoids from Cannabis sativa L. varieties
- Author
-
MA ElSohly, Mei Wang, Ikhlas A. Khan, Bharathi Avula, Z Mehemdic, YH Wang, Mohamed M. Radwan, and Amira S. Wanas
- Subjects
Drug ,Cannabigerol ,media_common.quotation_subject ,Pharmaceutical Science ,Tetrahydrocannabivarin ,Cannabicyclol ,Analytical Chemistry ,chemistry.chemical_compound ,Cannabichromene ,Drug Discovery ,medicine ,media_common ,Pharmacology ,biology ,Traditional medicine ,business.industry ,Organic Chemistry ,biology.organism_classification ,Biotechnology ,Complementary and alternative medicine ,chemistry ,Cannabinol ,Molecular Medicine ,Cannabis ,business ,Cannabidiol ,medicine.drug - Abstract
The cannabis plant is one of the oldest plants known in medicine, with many reported medicinal uses for > 5,000 years. However, the chemistry of the plant was not fully investigated till the chemical structure of the active component was determined in 1964. Since then, over 10,000 scientific citations could be found on cannabis and the cannabinoids. The legal status of cannabis in the United States has been in a state of controversy. Although the drug in classified as a Schedule I Controlled Substance, therefore, illegal to possess without appropriate Drug Enforcement Administration (DEA) Registration, 23 states and the District of Columbia have passed laws allowing the medical use of cannabis. Furthermore, Colorado and Washington State have passed legislations allowing recreational use of the drug as well. Over the past few years there has been a tremendous public interest in cannabis and cannabis oil, with many growers sprouting in all states with legal medical use. The medical community and the end users have also shown much interest in knowing the composition of the materials on the market. Several laboratories have reported on methods to determine the chemical composition of the cannabis products, particularly the Δ9-THC and the CBD levels, along with a few other cannabinoids. This report is directed toward an ultra-high performance liquid chromatographic method (UHPLC) with UV detection for the quantitation of eleven different cannabinoids with base line separation namely cannabidiolic acid, cannabigerolic acid, cannabigerol, cannabidiol, tetrahydrocannabivarin, cannabinol, Δ9-tetrahydrocannabinol, Δ8-tetrahydrocannabinol, cannabicyclol, cannabichromene, and Δ9-tetrahydrocannabinolic acid A. The method was validated and applied to the analysis and profiling of different varieties of cannabis produced at the UM Medicinal Plants Garden, as well as for the analysis of several confiscated cannabis preparations. The LOD, LOQ and linearity of the method were 0.2 – 0.4 µg/mL, 0.5 – 1.0 µg/mL and 1.0 – 200 µg/mL respectively, for all eleven cannabinoids.
- Published
- 2015
48. In vitro binding affinity to human CB1 and CB2 receptors and antimicrobial activity of volatile oil from high potency Cannabis sativa
- Author
-
Zlatko Mehmedic, Stephen J. Cutler, Amira S. Wanas, Mohamed M. Radwan, Ikhlas A. Khan, and MA ElSohly
- Subjects
Pharmacology ,Cannabinoid receptor ,business.industry ,Organic Chemistry ,Pharmaceutical Science ,Cannabis sativa ,Antimicrobial ,In vitro binding ,Analytical Chemistry ,Complementary and alternative medicine ,Drug Discovery ,Cannabinoid receptor type 2 ,Molecular Medicine ,Potency ,Medicine ,business - Published
- 2014
49. Isolation of eleven phenolic compounds from propolis (bee glue) collected in Alexandria, Egypt
- Author
-
Amira S. Wanas, Aly M. Metwally, Reham S. Ibrahim, Mohamed M. Radwan, MA ElSohly, and AA Seif El-Din
- Subjects
Pharmacology ,Propolis bee ,Complementary and alternative medicine ,Isolation (health care) ,Traditional medicine ,Organic Chemistry ,Drug Discovery ,Pharmaceutical Science ,Molecular Medicine ,Biology ,Analytical Chemistry - Published
- 2014
50. New secondary metabolites isolated from the leaves of Ixora undulata cultivated in Egypt
- Author
-
Hideaki Otsuka, Amira S. Wanas, S Sugimoto, Mohamed M. Radwan, MA ElSohly, T Mizuta, K Matsunami, and Kamel
- Subjects
Pharmacology ,Complementary and alternative medicine ,Traditional medicine ,Ixora undulata ,business.industry ,Organic Chemistry ,Drug Discovery ,Pharmaceutical Science ,Molecular Medicine ,Medicine ,business ,Analytical Chemistry - Published
- 2014
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