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Start Over Topic amino acids Language german
348 results

152. [Paper- and thin layer chromatography of amino acids in infusion solutions]

Author

H, Seydel and R, Voigt

Subjects
Threonine, Chromatography, Chromatography, Paper, Lysine, Phenylalanine, Glycine, Tryptophan, Valine, Arginine, Solutions, Methionine, Leucine, Methods, Histidine, Indicators and Reagents, Chromatography, Thin Layer, Amino Acids, Isoleucine
Published
1969

160. [Quantitative determination of amino acids from blood-stained filter papers using high-performance thin layer chromatography and a TLC-scanner (author's transl)]

Author

B M, Fetterroll and J, Sander

Subjects
Methods, Humans, Mass Screening, Chromatography, Thin Layer, Amino Acids, Child
Abstract

A method is described to determine amino acids by extraction from pieces of bloodstained and dried filter papers and following dansylation and separation by High-Performance Thin Layer Chromatography (HPTLC). Quantitative determination is possible by means of a TLC-Scanner measuring the fluorescent light of the dansylated amino acids. The possible use of this procedure as screening test is discussed.

Published
1979

169. [The primary structure of a monoclonic immunoglobulin-L-chain of subgroup IV of the kappa type (Bence-Jones protein Len.)]

Author

M, Schneider and N, Hilschmann

Subjects
Chromatography, Paper, Protein Conformation, Hydrolysis, Iodoacetates, Chromatography, Ion Exchange, Biological Evolution, Peptide Fragments, Epitopes, Immunoglobulin kappa-Chains, Chromatography, Gel, Chymotrypsin, Electrophoresis, Paper, Immunoglobulin Light Chains, Trypsin, Amino Acid Sequence, Amino Acids, Oxidation-Reduction, Bence Jones Protein
Abstract

The complete amino acid sequence of Bence-Jones protein Len. was established by sequential analysis of tryptic and chymotryptic peptides. The result of these experiments and the comparative sequence analysis with the other 17 completely determined kappa-proteins is incompatible with the serological typisation of protein Len. as a member of subgroup II: There are 18 positions in protein Len. than cannot be associated with any one of the subgroups kappaI, kappaII or kappaIII. Also the average amino acid exchange rate between protein Len. and these subgroups is in the same range as the average amino acid exchange rate between these subgroups. Therefore Bence-Jones protein Len. is the first completely determined representative of a new IV. kappa-type subgroup. The variability of immunoglobulins follows a structural principle in which the single point mutations responsible for the variability are linked. The present paper contains the exact analysis of the linked point mutations within the so far best-investigated subgroup, kappaI (12 completely sequenced proteins). These linked exchanges allow the arrangement of the kappaI proteins in 4 subgroups and their further subdivision. The regularity of this amino acid sequence pattern can only be explained by an evolutionary origin of antibody variability. On the basis of this evolutionary mechanism the relationship of immunoglobulins can be depicted in a phylogenetic tree. Such a tree was therefore constructed for the 18 completely determined Bence-Jones proteins of kappa-type, for the first time taking into account Bence-Jones protein Len. Its topology is in complete agreement with the results of the comparative sequence analysis.

Published
1975

170. [Pattern of antibody structure, the primary structure of monoclonal immunoglobulin L-chain of the lambda-type, subgroup IV (Bence-Jones protein Bau.) (author's transl)]

Author

K, Baczko, D, Braun, and N, Hilschmann

Subjects
Dansyl Compounds, Electrophoresis, Chromatography, Paper, Protein Conformation, Hydrolysis, Immunoglobulins, Carboxypeptidases, Peptide Chain Termination, Translational, Chromatography, DEAE-Cellulose, Molecular Weight, Hydrazines, Antibody Specificity, Chromatography, Gel, Humans, Electrophoresis, Paper, Trypsin, Amino Acid Sequence, Amino Acids, Oxidation-Reduction, Ultracentrifugation, Bence Jones Protein
Published
1974

171. [The primary structure of a human immunoglobin L-chain of kappa-type (Bence-Jones protein Scw) I: The tryptic peptides (author's transl)]

Author

M, Eulitz, D, Götze, and N, Hilschmann

Subjects
Dansyl Compounds, Chromatography, Paper, Hydrolysis, Thermolysin, Immunoglobulins, Carboxypeptidases, Aminopeptidases, Chromatography, DEAE-Cellulose, Pepsin A, Leucyl Aminopeptidase, Chymotrypsin, Humans, Electrophoresis, Paper, Trypsin, Amino Acid Sequence, Amino Acids, Peptides, Oxidation-Reduction, Bence Jones Protein
Published
1974

172. [Characterization of the light chains of immunoglobulins by fingerprinting]

Author

M, Braun and N, Hilschmann

Subjects
Dansyl Compounds, Autoanalysis, Chromatography, Paper, Hydrolysis, Tryptophan, Immunoglobulins, Arginine, Humans, Electrophoresis, Paper, Indicators and Reagents, Trypsin, Amino Acid Sequence, Amino Acids, Peptides, Immunoglobulin Fragments
Published
1972

173. [Constitution of the hemoglobin of the toad Bufo bufo spinosus. II]

Author

A A, Christomanos

Subjects
Alkylation, Formates, Chromatography, Paper, Macromolecular Substances, Ultraviolet Rays, Bufo bufo, Globins, Hemoglobins, Species Specificity, Spectrophotometry, Chromatography, Gel, Animals, Urea, Electrophoresis, Paper, Trypsin, Cysteine, Amino Acids, Anura, Peptides, Countercurrent Distribution, Oxidation-Reduction
Published
1972

174. [The composition of the blood serum of healthy Ethiopians]

Author

E, Gründig, M, Simanyi, R F, Maruna, and K, Weithaler

Subjects
Adult, Male, Paper, Chromatography, Blood Protein Disorders, Chromatography, Paper, Racial Groups, Blood Protein Electrophoresis, Malaria, Europe, Humans, Indicators and Reagents, Ethiopia, gamma-Globulins, Amino Acids
Published
1970

175. [Study of the acetyltransferase component of fatty acid synthetase of yeast]

Author

J, Ziegenhorn, R, Niedermeier, C, Nüssler, and F, Lynen

Subjects
Binding Sites, Chromatography, Paper, Fatty Acids, Iodoacetates, Carboxypeptidases, Saccharomyces cerevisiae, Chromatography, Ion Exchange, Pepsin A, Acetyltransferases, Ethylmaleimide, Chromatography, Gel, Coenzyme A, Electrophoresis, Paper, Trypsin, Amino Acid Sequence, Chromatography, Thin Layer, Amino Acids, Fatty Acid Synthases, Carrier Proteins, Peptides
Published
1972

176. [Principle of antibody structure. The structure of a monoclonal kappa I-type immunoglobulin L-chain (Bence Jones protein Bi). II. Isolation and characterization of the tryptic peptides]

Author

M, Braun, W, Leibold, and N, Hilschmann

Subjects
Dansyl Compounds, Electrophoresis, Male, Autoanalysis, Chromatography, Paper, Hydantoins, Carboxypeptidases, Chromatography, Ion Exchange, Chromatography, Gel, Humans, Electrophoresis, Paper, Indicators and Reagents, Trypsin, Amino Acids, Peptides, Immunoglobulin Fragments, Bence Jones Protein, Plasmacytoma
Published
1972

177. [Studies on L-asparaginase, inactivation and modification of the enzyme by N-bromosuccinimide and substrate specificity of the reaction (author's transl)]

Author

U, Menge and L, Jaenicke

Subjects
Chromatography, Gas, Hydrolysis, Temperature, Ninhydrin, Succinimides, Buffers, Hydrogen-Ion Concentration, Bromine, Kinetics, Spectrometry, Fluorescence, Escherichia coli, Asparaginase, Tyrosine, Electrophoresis, Paper, Histidine, Spectrophotometry, Ultraviolet, Amino Acids, Asparagine, Peptides, Dialysis, Ultracentrifugation
Published
1974

179. [The sequence of beta-lactoglobulin (author's transl)]

Author

G, Braunitzer, R, Chen, B, Schrank, and A, Stangl

Subjects
Autoanalysis, Chromatography, Paper, Lactoglobulins, Chromatography, Ion Exchange, Pepsin A, Peptide Fragments, Evaluation Studies as Topic, Methods, Animals, Cattle, Female, Spectrophotometry, Ultraviolet, Trypsin, Amino Acid Sequence, Cyanogen Bromide, Amino Acids, Oxidation-Reduction
Published
1973

183. [Preparation and properties of mixed disulfides of insulin with glutathione and thioglycollic acid (author's transl)]

Author

W D, Busse and H G, Gattner

Subjects
Electrophoresis, Binding Sites, Passive Cutaneous Anaphylaxis, Chromatography, Ion Exchange, Glutathione, Peptide Fragments, Evaluation Studies as Topic, Thioglycolates, Chromatography, Gel, Methods, Animals, Chymotrypsin, Insulin, Cattle, Electrophoresis, Paper, Spectrophotometry, Ultraviolet, Disulfides, Amino Acids, Cellulose, Oxidation-Reduction, Protein Binding
Published
1973

197. [On an endopeptidase, hydrolyzing native proteins in the physiological range, from the intestines of Tenebrio molitor. Purification and characterization of the enzyme]

Author

G, Pfleiderer and R, Zwilling

Subjects
Electrophoresis, Alanine, Insecta, Chemical Phenomena, L-Lactate Dehydrogenase, Chromatography, Paper, Glyceraldehyde-3-Phosphate Dehydrogenases, In Vitro Techniques, NAD, Intestines, Chemistry, Ribonucleases, Spectrophotometry, Animals, Insulin, Chromatography, Thin Layer, Amino Acids, Peptides, Peptide Hydrolases
Published
1966