20 results on '"Maiolo, D."'
Search Results
2. THE MISSENSE CAV-3/P104L MUTATION, INVOLVED IN THE ONSET OF LIMB GIRDLE MUSCULAR DYSTROPHY 1-C, CONFERS AN IMMATURE SIGNATURE TO C2C12 MYOBLASTS
- Author
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Stoppani, E, Maiolo, D, Rossi, S, Meacci, E, Penna, F, Costelli, P, and Fanzani, Alessandro
- Published
- 2009
3. Low adiponectin levels, cardiovascular (CV) risk factor and early vessel damage in damage in obese children and adolescent
- Author
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Rossetti, P., Alagona, C., Nigro, A., Leonardi, D., Maiolo, D., Purrello, Francesco, and Frittitta, Lucia
- Published
- 2009
4. Relationship to insulin resistance of the ATP III and IDF diagnostic criteria for identification of the metabolic syndrome
- Author
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Baratta, R., Maiolo, D., Farina, M. G., Puglisi, C., Vigneri, R., and Frittitta, Lucia
- Published
- 2007
5. Prevalence of nonalcoholic fatty liver disease (NAFLD) and metabolic syndrome (MS) among obese children and adolescents and main clinical and metabolic features
- Author
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Nigro, A., Russello, M., Rossetti, P., Maiolo, D., Degano, C., Baratta, R., Vigneri, R., and Frittitta, Lucia
- Published
- 2007
6. Halogen bonding modulates hydrogel formation from Fmoc amino acids.
- Author
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Pizzi, A., Lascialfari, L., Demitri, N., Bertolani, A., Maiolo, D., Carretti, E., and Metrangolo, P.
- Subjects
SINGLE crystals ,PHENYLALANINE ,AMINO acids ,GELATION ,POLARIZABILITY (Electricity) - Abstract
The single crystal X-ray structures of a series of p-halogenated Fmoc phenylalanines highlight the role of halogen bonding in their supramolecular organization in the solid state. The amino acids showing the occurrence of halogen bonding resulted in gels with the strongest mechanical properties. All gelation parameters could be ranked according to halogen atom polarizability, i.e., halogen bond strength. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
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7. Halogenation of the N -Terminus Tyrosine 10 Promotes Supramolecular Stabilization of the Amyloid-β Sequence 7-12.
- Author
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Maiolo D, Pizzi A, Gori A, Gazzera L, Demitri N, Genoni A, Baggi F, Moda F, Terraneo G, Baldelli Bombelli F, Metrangolo P, and Resnati G
- Subjects
- Amino Acid Sequence, Amino Acids chemistry, Crystallization, Halogenation, Hydrogen Bonding, Molecular Conformation, Oxidation-Reduction, Amyloid beta-Peptides chemistry, Bromine chemistry, Tyrosine chemistry
- Abstract
Here, we demonstrate that introduction of halogen atoms at the tyrosine 10 phenol ring of the DSGYEV sequence derived from the flexible amyloid-β N -terminus, promotes its self-assembly in the solid state. In particular, we report the crystal structures of two halogen-modified sequences, which we found to be stabilized in the solid state by halogen-mediated interactions. The structural study is corroborated by Non-Covalent Interaction (NCI) analysis. Our results prove that selective halogenation of an amino acid enhances the supramolecular organization of otherwise unstructured biologically-relevant sequences. This method may develop as a general strategy for stabilizing highly polymorphic peptide regions., Competing Interests: The authors declare no conflict of interest., (© 2020 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.)
- Published
- 2020
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8. The polyplex, protein corona, cell interplay: Tips and drawbacks.
- Author
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Maiolo D, Colombo J, Beretta J, Malloggi C, Candiani G, and Baldelli Bombelli F
- Subjects
- Cell Survival genetics, DNA genetics, Electrophoresis, Polyacrylamide Gel, HeLa Cells, Humans, Protein Corona metabolism, Static Electricity, DNA chemistry, Gene Transfer Techniques, Polyethyleneimine chemistry, Protein Corona chemistry
- Abstract
Polyplexes (PX) are soft materials, obtained by blending polycations and nucleic acids, designed for gene delivery applications. While much is known about the transfection properties of PX, their protein corona, the biomolecules interacting with colloids once in a biological environment, represents an underlooked parameter in gene transfection. In this study, linear and branched polyethylenimines (lPEI and bPEI), the golden standard among non-viral vectors, were selected and used throughout the work: their physicochemical properties and protein corona when complexed to DNA were studied and linked to the toxicity and transfection efficiency arisen upon their delivery to cells. Interestingly, lPEIDNA and bPEIDNA complexes were characterized by similar physicochemical features, but different biological behavior. In fact, the biological milieu where cells and PX interact greatly influences their size, stability and transfection abilities. Using PX as a soft material model system, we spotlighted structure-activity relationships and methodologies that can help interpret their biological behavior and guide future studies in the field., (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Published
- 2018
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9. Efficient Encapsulation of Fluorinated Drugs in the Confined Space of Water-Dispersible Fluorous Supraparticles.
- Author
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Pigliacelli C, Maiolo D, Nonappa, Haataja JS, Amenitsch H, Michelet C, Sánchez Moreno P, Tirotta I, Metrangolo P, and Baldelli Bombelli F
- Subjects
- Alkynes, Cyclopropanes, Gold chemistry, Halogenation, Macromolecular Substances chemistry, Metal Nanoparticles chemistry, Particle Size, Water chemistry, Benzoxazines chemistry, Celecoxib chemistry, Fluorine chemistry, Leflunomide chemistry
- Abstract
Fluorophobic-driven assemblies of gold nanomaterials were stabilized into water-dispersible fluorous supraparticles by the film-forming protein hydrophobin II. The strategy makes use of fluorous nanomaterials of different dimensions to engineer size and inner functionalization of the resulting confined space. The inner fluorous compartments allow efficient encapsulation and transport of high loadings of partially fluorinated drug molecules in water., (© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Published
- 2017
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10. Bioreducible Hydrophobin-Stabilized Supraparticles for Selective Intracellular Release.
- Author
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Maiolo D, Pigliacelli C, Sánchez Moreno P, Violatto MB, Talamini L, Tirotta I, Piccirillo R, Zucchetti M, Morosi L, Frapolli R, Candiani G, Bigini P, Metrangolo P, and Baldelli Bombelli F
- Subjects
- Animals, Antineoplastic Agents, Phytogenic pharmacokinetics, Cell Line, Tumor, Drug Liberation, Female, Humans, Mice, Inbred BALB C, Paclitaxel pharmacokinetics, Antineoplastic Agents, Phytogenic administration & dosage, Fungal Proteins chemistry, Gold chemistry, Hypocrea chemistry, Metal Nanoparticles chemistry, Paclitaxel administration & dosage
- Abstract
One of the main hurdles in nanomedicine is the low stability of drug-nanocarrier complexes as well as the drug delivery efficiency in the region-of-interest. Here, we describe the use of the film-forming protein hydrophobin HFBII to organize dodecanethiol-protected gold nanoparticles (NPs) into well-defined supraparticles (SPs). The obtained SPs are exceptionally stable in vivo and efficiently encapsulate hydrophobic drug molecules. The HFBII film prevents massive release of the encapsulated drug, which, instead, is activated by selective SP disassembly triggered intracellularly by glutathione reduction of the protein film. As a consequence, the therapeutic efficiency of an encapsulated anticancer drug is highly enhanced (2 orders of magnitude decrease in IC
50 ). Biodistribution and pharmacokinetics studies demonstrate the high stability of the loaded SPs in the bloodstream and the selective release of the payloads once taken up in the tissues. Overall, our results provide a rationale for the development of bioreducible and multifunctional nanomedicines.- Published
- 2017
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11. Long-Pentraxin 3 Derivative as a Small-Molecule FGF Trap for Cancer Therapy.
- Author
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Ronca R, Giacomini A, Di Salle E, Coltrini D, Pagano K, Ragona L, Matarazzo S, Rezzola S, Maiolo D, Torrella R, Moroni E, Mazzieri R, Escobar G, Mor M, Colombo G, and Presta M
- Subjects
- Animals, Blotting, Western, C-Reactive Protein metabolism, Cell Cycle drug effects, Cell Cycle genetics, Cell Line, Tumor, Cell Survival drug effects, Cell Survival genetics, Cells, Cultured, Female, Fibroblast Growth Factors metabolism, Fibroblast Growth Factors pharmacology, Humans, Kaplan-Meier Estimate, Male, Mice, Inbred C57BL, Mice, Nude, Mice, Transgenic, Molecular Structure, Neoplasms metabolism, Neoplasms therapy, Neovascularization, Pathologic genetics, Neovascularization, Pathologic metabolism, Reverse Transcriptase Polymerase Chain Reaction, Serum Amyloid P-Component metabolism, Small Molecule Libraries chemistry, Small Molecule Libraries pharmacology, Tumor Burden drug effects, Tumor Burden genetics, Xenograft Model Antitumor Assays methods, C-Reactive Protein genetics, Fibroblast Growth Factors genetics, Gene Expression Regulation, Neoplastic, Neoplasms genetics, Serum Amyloid P-Component genetics
- Abstract
The fibroblast growth factor (FGF)/FGF receptor (FGFR) system plays a crucial role in cancer by affecting tumor growth, angiogenesis, drug resistance, and escape from anti-angiogenic anti-vascular endothelial growth factor therapy. The soluble pattern recognition receptor long-pentraxin 3 (PTX3) acts as a multi-FGF antagonist. Here we demonstrate that human PTX3 overexpression in transgenic mice driven by the Tie2 promoter inhibits tumor growth, angiogenesis, and metastasis in heterotopic, orthotopic, and autochthonous FGF-dependent tumor models. Using pharmacophore modeling of the interaction of a minimal PTX3-derived FGF-binding pentapeptide with FGF2, we identified a small-molecule chemical (NSC12) that acts as an extracellular FGF trap with significant implications in cancer therapy., (Copyright © 2015 Elsevier Inc. All rights reserved.)
- Published
- 2015
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12. Colorimetric nanoplasmonic assay to determine purity and titrate extracellular vesicles.
- Author
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Maiolo D, Paolini L, Di Noto G, Zendrini A, Berti D, Bergese P, and Ricotta D
- Subjects
- Eye chemistry, Humans, Colorimetry economics, Exosomes chemistry, Gold chemistry, Metal Nanoparticles chemistry, Nanotechnology economics, Proteins analysis
- Abstract
Extracellular Vesicles (EVs) - cell secreted vesicles that carry rich molecular information of the parental cell and constitute an important mode of intercellular communication - are becoming a primary topic in translational medicine. EVs (that comprise exosomes and microvesicles/microparticles) have a size ranging from 40 nm to 1 μm and share several physicochemical proprieties, including size, density, surface charge, and light interaction, with other nano-objects present in body fluids, such as single and aggregated proteins. This makes separation, titration, and characterization of EVs challenging and time-consuming. Here we present a cost-effective and fast colorimetric assay for probing by eye protein contaminants and determine the concentration of EV preparations, which exploits the synergy between colloidal gold nanoplasmonics, nanoparticle-protein corona, and nanoparticle-membrane interaction. The assay hits a limit of detection of protein contaminants of 5 ng/μL and has a dynamic range of EV concentration ranging from 35 fM to 35 pM, which matches the typical range of EV concentration in body fluids. This work provides the first example of the exploitation of the nanoparticle-protein corona in analytical chemistry.
- Published
- 2015
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13. Nanomedicine delivery: does protein corona route to the target or off road?
- Author
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Maiolo D, Del Pino P, Metrangolo P, Parak WJ, and Baldelli Bombelli F
- Subjects
- Drug Administration Routes, Nanomedicine, Proteins chemistry
- Abstract
Nanomedicine aims to find novel solutions for urgent biomedical needs. Despite this, one of the most challenging hurdles that nanomedicine faces is to successfully target therapeutic nanoparticles to cells of interest in vivo. As for any biomaterials, once in vivo, nanoparticles can interact with plasma biomolecules, forming new entities for which the name protein coronas (PCs) have been coined. The PC can influence the in vivo biological fate of a nanoparticle. Thus for guaranteeing the desired function of an engineered nanomaterial in vivo, it is crucial to dissect its PC in terms of formation and evolution within the body. In this contribution we will review the 'good' and 'bad' sides of the PC, starting from the scientific aspects to the technological applications.
- Published
- 2015
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14. Surfactant titration of nanoparticle-protein corona.
- Author
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Maiolo D, Bergese P, Mahon E, Dawson KA, and Monopoli MP
- Subjects
- Electrophoresis, Gel, Two-Dimensional, Humans, Nanoparticles chemistry, Proteins chemistry, Surface-Active Agents chemistry
- Abstract
Nanoparticles (NP), when exposed to biological fluids, are coated by specific proteins that form the so-called protein corona. While some adsorbing proteins exchange with the surroundings on a short time scale, described as a "dynamic" corona, others with higher affinity and long-lived interaction with the NP surface form a "hard" corona (HC), which is believed to mediate NP interaction with cellular machineries. In-depth NP protein corona characterization is therefore a necessary step in understanding the relationship between surface layer structure and biological outcomes. In the present work, we evaluate the protein composition and stability over time and we systematically challenge the formed complexes with surfactants. Each challenge is characterized through different physicochemical measurements (dynamic light scattering, ζ-potential, and differential centrifugal sedimentation) alongside proteomic evaluation in titration type experiments (surfactant titration). 100 nm silicon oxide (Si) and 100 nm carboxylated polystyrene (PS-COOH) NPs cloaked by human plasma HC were titrated with 3-[(3-Cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS, zwitterionic), Triton X-100 (nonionic), sodium dodecyl sulfate (SDS, anionic), and dodecyltrimethylammonium bromide (DTAB, cationic) surfactants. Composition and density of HC together with size and ζ-potential of NP-HC complexes were tracked at each step after surfactant titration. Results on Si NP-HC complexes showed that SDS removes most of the HC, while DTAB induces NP agglomeration. Analogous results were obtained for PS NP-HC complexes. Interestingly, CHAPS and Triton X-100, thanks to similar surface binding preferences, enable selective extraction of apolipoprotein AI (ApoAI) from Si NP hard coronas, leaving unaltered the dispersion physicochemical properties. These findings indicate that surfactant titration can enable the study of NP-HC stability through surfactant variation and also selective separation of certain proteins from the HC. This approach thus has an immediate analytical value as well as potential applications in HC engineering.
- Published
- 2014
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15. Interaction of nanoparticles with lipid membranes: a multiscale perspective.
- Author
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Montis C, Maiolo D, Alessandri I, Bergese P, and Berti D
- Subjects
- Diffusion, Materials Testing, Models, Molecular, Gold chemistry, Lipid Bilayers chemistry, Metal Nanoparticles chemistry, Metal Nanoparticles ultrastructure, Models, Chemical
- Abstract
Freestanding lipid bilayers were challenged with 15 nm Au nanospheres either coated by a citrate layer or passivated by a protein corona. The effect of Au nanospheres on the bilayer morphology, permeability and fluidity presents strong differences or similarities, depending on the observation length scale, from the colloidal to the molecular domains. These findings suggest that the interaction between nanoparticles and lipid membranes should be conveniently treated as a multiscale phenomenon.
- Published
- 2014
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16. Nanomechanics of surface DNA switches probed by captive contact angle.
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Maiolo D, Federici S, Ravelli L, Depero LE, Hamad-Schifferli K, and Bergese P
- Subjects
- Humans, Nanotechnology methods, Potassium chemistry, Sodium chemistry, Surface Properties, Aptamers, Nucleotide chemistry, DNA, Single-Stranded chemistry, Gold chemistry, Thrombin
- Abstract
Self-assembled monolayers of Thrombin Binding Aptamers (TBA) were prepared on gold surfaces with typical surface densities of close-packed ssDNA (4×10(12) and 8×10(12)molecules/cm(2)). CONtact Angle MOlecular REcognition (CONAMORE) in captive bubble geometry was then assessed to scan the surface work triggered by TBAs when switching from the elongated to the G-quadruplex conformation upon binding with Na(+) or K(+) cations. We found Na(+) and K(+) could induce comparable linear to G-quadruplex strokes, and resulted in values for surface work of ~-70 pN nm/molecule (~18 kBT). The strokes change the in-plane van der Waals and weak electrostatic interactions and accumulate to result in macroscopic surface work. Micro- to macroscopic translation strongly depends on the nature of the cation and TBA surface density. In particular, the K(+) stimulus triggers a macroscopic surface work of -2.2±0.2 and -5.3±0.2 mN/m for low and high packed monolayers, respectively, while Na(+) triggers up to -6.7±1.0 mN/m in the highly packed monolayer, but creates negligible work for the low packed monolayer. These results show that CONAMORE can contribute important information for the development of devices based on DNA switches, and ultimately help address some of the open challenges for DNA-based nanomachinery., (Copyright © 2013 Elsevier Inc. All rights reserved.)
- Published
- 2013
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17. Role of nanomechanics in canonical and noncanonical pro-angiogenic ligand/VEGF receptor-2 activation.
- Author
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Maiolo D, Mitola S, Leali D, Oliviero G, Ravelli C, Bugatti A, Depero LE, Presta M, and Bergese P
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- Cytokines, Fluorescence Resonance Energy Transfer, Humans, Intercellular Signaling Peptides and Proteins metabolism, Ligands, Vascular Endothelial Growth Factor A metabolism, Mechanical Phenomena, Nanotechnology methods, Neovascularization, Physiologic, Surface Plasmon Resonance methods, Vascular Endothelial Growth Factor Receptor-2 metabolism
- Abstract
Vascular endothelial growth factor receptor-2 (VEGFR2) is an endothelial cell receptor that plays a pivotal role in physiologic and pathologic angiogenesis and is a therapeutic target for angiogenesis-dependent diseases, including cancer. By leveraging on a dedicated nanomechanical biosensor, we investigated the nanoscale mechanical phenomena intertwined with VEGFR2 surface recognition by its prototypic ligand VEGF-A and its noncanonical ligand gremlin. We found that the two ligands bind the immobilized extracellular domain of VEGFR2 (sVEGFR2) with comparable binding affinity. Nevertheless, they interact with sVEGFR2 with different binding kinetics and drive different in-plane piconewton intermolecular forces, suggesting that the binding of VEGF-A or gremlin induces different conformational changes in sVEGFR2. These behaviors can be effectively described in terms of a different "nanomechanical affinity" of the two ligands for sVEGFR2, about 16-fold higher for VEGF-A with respect to gremlin. Such nanomechanical differences affect the biological activity driven by the two angiogenic factors in endothelial cells, as evidenced by a more rapid VEGFR2 clustering and a more potent mitogenic response triggered by VEGF-A in respect to gremlin. Together, these data point to surface intermolecular interactions on cell membrane between activated receptors as a key modulator of the intracellular signaling cascade.
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- 2012
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18. On the thermodynamics of biomolecule surface transformations.
- Author
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Federici S, Oliviero G, Maiolo D, Depero LE, Colombo I, and Bergese P
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- Biomechanical Phenomena, Biosensing Techniques, Humans, Ligands, Models, Chemical, Nanotechnology, Protein Binding, Solutions, Surface Properties, Biological Factors chemistry, Nanoparticles chemistry, Receptors, Cell Surface chemistry, Thermodynamics
- Abstract
Biological surface science is receiving great and renewed attention owing the rising interest in applications of nanoscience and nanotechnology to biological systems, with horizons that range from nanomedicine and biomimetic photosynthesis to the unexpected effects of nanomaterials on health and environment. Biomolecule surface transformations are among the fundamental aspects of the field that remain elusive so far and urgently need to be understood to further the field. Our recent findings indicate that surface thermodynamics can give a substantial contribution toward this challenging goal. In the first part of the article, we show that biomolecule surface transformations can be framed by a general and simple thermodynamic model. Then, we explore its effectiveness by addressing some typical cases, including ligand-receptor surface binding, protein thin film machines, nanomechanical aspects of the biomolecule-nanoparticle interface and nanomechanical biosensors., (Copyright © 2012 Elsevier Inc. All rights reserved.)
- Published
- 2012
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19. Point mutated caveolin-3 form (P104L) impairs myoblast differentiation via Akt and p38 signalling reduction, leading to an immature cell signature.
- Author
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Stoppani E, Rossi S, Meacci E, Penna F, Costelli P, Bellucci A, Faggi F, Maiolo D, Monti E, and Fanzani A
- Subjects
- Animals, Caveolin 3 metabolism, Cell Differentiation drug effects, Cell Fusion, Cell Line, Gene Expression Regulation, Hydroxamic Acids pharmacology, Insulin-Like Growth Factor I pharmacology, Mice, Myoblasts drug effects, Myoblasts metabolism, Protein Synthesis Inhibitors pharmacology, Rats, Signal Transduction, Transforming Growth Factor beta metabolism, Caveolin 3 genetics, Myoblasts cytology, Point Mutation, Proto-Oncogene Proteins c-akt metabolism, p38 Mitogen-Activated Protein Kinases metabolism
- Abstract
Unbalanced levels of caveolin-3 (Cav3) are involved in muscular disorders. In the present study we show that differentiation of immortalized myoblasts is affected by either lack or overexpression of Cav3. Nevertheless, depletion of Cav3 induced by delivery of the dominant-negative Cav3 (P104L) form elicited a more severe phenotype, characterized by the simultaneous attenuation of the Akt and p38 signalling networks, leading to an immature cell and molecular signature. Accordingly, differentiation of myoblasts harbouring Cav3 (P104L) was improved by countering the reduced Akt and p38 signalling network via administration of IGF-1 or trichostatin A. Furthermore, loss of Cav3 correlated with a deregulation of the TGF-β-induced Smad2 and Erk1/2 pathways, confirming that Cav3 controls TGF-β signalling at the plasma membrane. Overall, these data suggest that loss of Cav3, primarily causing attenuation of both Akt and p38 pathways, contributes to impair myoblast fusion., (Copyright © 2010 Elsevier B.V. All rights reserved.)
- Published
- 2011
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20. Nanoliter contact angle probes tumor angiogenic ligand-receptor protein interactions.
- Author
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Olivero G, Maiolo D, Leali D, Federici S, Depero LE, Presta M, Mitola S, and Bergese P
- Subjects
- Endothelial Growth Factors metabolism, Enzyme-Linked Immunosorbent Assay, Humans, Immobilized Proteins, In Vitro Techniques, Ligands, Peptides, Cyclic metabolism, Recombinant Fusion Proteins metabolism, Recombinant Proteins metabolism, Thermodynamics, Vascular Endothelial Growth Factor A metabolism, Angiogenic Proteins metabolism, Protein Interaction Mapping methods, Surface Plasmon Resonance methods
- Abstract
Any molecular recognition reaction supported by a solid phase drives a specific change of the solid-solution interfacial tension. Sessile contact angle (CA) experiments can be readily used to track this thermodynamic parameter, prompting this well-known technique to be reinvented as an alternative, easy-access and label-free way to probe and study molecular recognition events. Here we deploy this technique, renamed for this application CONAMORE (CONtact Angle MOlecular REcognition), to study the interaction of the tumor-derived pro-angiogenic vascular endothelial growth factor-A (VEGF-A) with the extracellular domain of its receptor VEGFR2. We show that CONAMORE recognizes the high affinity binding of VEGF-A at nanomolar concentrations to surface-immobilized VEGFR2 regardless of the presence of a ten-fold excess of a non-specific interacting protein, and that it further proofs its specificity and reliability on competitive binding experiments involving neutralizing anti-VEGF-A antibodies. Finally, CONAMORE shows the outstanding capability to detect the specific interaction between VEGFR2 and low molecular weight ligands, such as Cyclo-VEGI, a VEGFR2 antagonist cyclo-peptide, that weighs about 2 kDa., (Copyright © 2010 Elsevier B.V. All rights reserved.)
- Published
- 2010
- Full Text
- View/download PDF
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