39 results on '"Kissa, Karima"'
Search Results
2. Phosphatidylinositol-3 kinase signaling controls survival and stemness of hematopoietic stem and progenitor cells
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Blokzijl-Franke, Sasja, Ponsioen, Bas, Schulte-Merker, Stefan, Herbomel, Philippe, Kissa, Karima, Choorapoikayil, Suma, and den Hertog, Jeroen
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- 2021
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3. Macrophage morphological plasticity and migration is Rac signalling and MMP9 dependant
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Travnickova, Jana, Nhim, Sandra, Abdellaoui, Naoill, Djouad, Farida, Nguyen-Chi, Maï, Parmeggiani, Andrea, and Kissa, Karima
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- 2021
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4. Modeling and live imaging of mechanical instabilities in the zebrafish aorta during hematopoiesis
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Chalin, Dmitrii, Bureau, Charlotte, Parmeggiani, Andrea, Rochal, Sergei, Kissa, Karima, and Golushko, Ivan
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- 2021
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5. Author Correction: Modeling and live imaging of mechanical instabilities in the zebrafish aorta during hematopoiesis
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Chalin, Dmitrii, Bureau, Charlotte, Parmeggiani, Andrea, Rochal, Sergei, Kissa, Karima, and Golushko, Ivan
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- 2021
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6. Mitochondrial morphology is associated with respiratory chain uncoupling in autism spectrum disorder
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Frye, Richard E., Lionnard, Loïc, Singh, Indrapal, Karim, Mohammad A., Chajra, Hanane, Frechet, Mathilde, Kissa, Karima, Racine, Victor, Ammanamanchi, Amrit, McCarty, Patrick John, Delhey, Leanna, Tippett, Marie, Rose, Shannon, and Aouacheria, Abdel
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- 2021
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7. Mechanical instabilities of aorta drive blood stem cell production: a live study
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Poullet, Nausicaa, Golushko, Ivan, Lorman, Vladimir, Travnickova, Jana, Bureau, Charlotte, Chalin, Dmitrii, Rochal, Sergei, Parmeggiani, Andrea, and Kissa, Karima
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- 2020
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8. HRAS germline mutations impair LKB1/AMPK signaling and mitochondrial homeostasis in Costello syndrome models
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Dard, Laetitia, Hubert, Christophe, Esteves, Pauline, Blanchard, Wendy, About, Ghina Bou, Baldasseroni, Lyla, Dumon, Elodie, Angelini, Chloe, Delourme, Megane, Guyonnet-Duperat, Veronique, Claverol, Stephane, Fontenille, Laura, Kissa, Karima, Seguela, Pierre-Emmanuel, Thambo, Jean-Benoit, Nicolas, Levy, Herault, Yann, Bellance, Nadege, Amoedo, Nivea Dias, Magdinier, Frederique, Sorg, Tania, Lacombe, Didier, and Rossignol, Rodrigue
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Gene mutations -- Research ,Medical research ,Medicine, Experimental ,Costello syndrome -- Development and progression ,Homeostasis -- Genetic aspects -- Health aspects ,Mitochondria -- Health aspects -- Genetic aspects ,Protein kinases -- Genetic aspects -- Health aspects ,Cellular signal transduction -- Research ,Health care industry - Abstract
Germline mutations that activate genes in the canonical RAS/MAPK signaling pathway are responsible for rare human developmental disorders known as RASopathies. Here, we analyzed the molecular determinants of Costello syndrome (CS) using a mouse model expressing HRAS p.G12S, patient skin fibroblasts, hiPSC-derived human cardiomyocytes, a HRAS p.G12V zebrafish model, and human fibroblasts expressing lentiviral constructs carrying HRAS p.G12S or HRAS p.G12A mutations. The findings revealed alteration of mitochondrial proteostasis and defective oxidative phosphorylation in the heart and skeletal muscle of CS mice that were also found in the cell models of the disease. The underpinning mechanisms involved the inhibition of the AMPK signaling pathway by mutant forms of HRAS, leading to alteration of mitochondrial proteostasis and bioenergetics. Pharmacological activation of mitochondrial bioenergetics and quality control restored organelle function in HRAS p.G12A and p.G12S cell models, reduced left ventricle hypertrophy in CS mice, and diminished the occurrence of developmental defects in the CS zebrafish model. Collectively, these findings highlight the importance of mitochondrial proteostasis and bioenergetics in the pathophysiology of RASopathies and suggest that patients with CS may benefit from treatment with mitochondrial modulators., Introduction Germline mutations that activate RAS/MAPK signaling are responsible for the 'RASopathies,' a group of rare human developmental diseases that affect more than 400,000 individuals in the United States alone. [...]
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- 2022
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9. Quantitative imaging and semiotic phenotyping of mitochondrial network morphology in live human cells.
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Charrasse, Sophie, Racine, Victor, Saint-Omer, Charlotte, Poquillon, Titouan, Lionnard, Loïc, Ledru, Marine, Gonindard, Christophe, Delaunois, Sandrine, Kissa, Karima, Frye, Richard E., Pastore, Manuela, Reynes, Christelle, Frechet, Mathilde, Chajra, Hanane, and Aouacheria, Abdel
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KERATINOCYTE differentiation ,MITOCHONDRIA ,ORGANELLES ,HUMAN cell culture ,CYTOLOGY ,MORPHOLOGY - Abstract
The importance of mitochondria in tissue homeostasis, stress responses and human diseases, combined to their ability to transition between various structural and functional states, makes them excellent organelles for monitoring cell health. There is therefore a need for technologies to accurately analyze and quantify changes in mitochondrial organization in a variety of cells and cellular contexts. Here we present an innovative computerized method that enables accurate, multiscale, fast and cost-effective analysis of mitochondrial shape and network architecture from confocal fluorescence images by providing more than thirty features. In order to facilitate interpretation of the quantitative results, we introduced two innovations: the use of Kiviat-graphs (herein named MitoSpider plots) to present highly multidimensional data and visualization of the various mito-cellular configurations in the form of morphospace diagrams (called MitoSigils). We tested our fully automated image analysis tool on rich datasets gathered from live normal human skin cells cultured under basal conditions or exposed to specific stress including UVB irradiation and pesticide exposure. We demonstrated the ability of our proprietary software (named MitoTouch) to sensitively discriminate between control and stressed dermal fibroblasts, and between normal fibroblasts and other cell types (including cancer tissue-derived fibroblasts and primary keratinocytes), showing that our automated analysis captures subtle differences in morphology. Based on this novel algorithm, we report the identification of a protective natural ingredient that mitigates the deleterious impact of hydrogen peroxide (H2O2) on mitochondrial organization. Hence we conceived a novel wet-plus-dry pipeline combining cell cultures, quantitative imaging and semiotic analysis for exhaustive analysis of mitochondrial morphology in living adherent cells. Our tool has potential for broader applications in other research areas such as cell biology and medicine, high-throughput drug screening as well as predictive and environmental toxicology. [ABSTRACT FROM AUTHOR]
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- 2024
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10. Retrograde Trans-Synaptic Transfer of Green Fluorescent Protein Allows the Genetic Mapping of Neuronal Circuits in Transgenic Mice
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Maskos, Uwe, Kissa, Karima, St. Cloment, Cécile, and Brûlet, Philippe
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- 2002
11. Whole embryo culture, transcriptomics and RNA interference identify TBX1 and FGF11 as novel regulators of limb development in the mouse
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Tejedor, Gautier, Laplace-Builhé, Béryl, Luz-Crawford, Patricia, Assou, Said, Barthelaix, Audrey, Mathieu, Marc, Kissa, Karima, Jorgensen, Christian, Collignon, Jérôme, Chuchana, Paul, and Djouad, Farida
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- 2020
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12. The ROSA-Like Prophage Colonizing Staphylococcus aureus Promotes Intracellular Survival, Biofilm Formation, and Virulence in a Chronic Wound Environment.
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Ahmad-Mansour, Nour, Plumet, Lucile, Pouget, Cassandra, Kissa, Karima, Dunyach-Remy, Catherine, Sotto, Albert, Lavigne, Jean-Philippe, and Molle, Virginie
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CHRONIC wounds & injuries ,STAPHYLOCOCCUS aureus ,DIABETIC foot ,COLONIZATION (Ecology) ,MICROCOCCACEAE ,BIOFILMS ,LABORATORY zebrafish - Abstract
Background The transition from colonization to invasion is critical in diabetic foot ulcer (DFU). Staphylococcus aureus can colonize DFU, or invade the underlying tissues, causing serious infections. The ROSA-like prophage has previously been implicated in strain colonization characteristics of S aureus isolates in uninfected ulcers. Methods In this study, we investigated this prophage in the S aureus -colonizing strain using an in vitro chronic wound medium mimicking the chronic wound environment. Results Chronic wound medium reduced bacterial growth and increased biofilm formation and virulence in a zebrafish model. Conclusions The ROSA-like prophage promoted intracellular survival of S aureus -colonizing strain in macrophages, keratinocytes, and osteoblasts. [ABSTRACT FROM AUTHOR]
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- 2023
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13. Pivotal role of Pten in the balance between proliferation and differentiation of hematopoietic stem cells in zebrafish
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Choorapoikayil, Suma, Kers, Rianne, Herbomel, Philippe, Kissa, Karima, and den Hertog, Jeroen
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- 2014
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14. Mycobacterium abscessus cording prevents phagocytosis and promotes abscess formation
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Bernut, Audrey, Herrmann, Jean-Louis, Kissa, Karima, Dubremetz, Jean-François, Gaillard, Jean-Louis, Lutfalla, Georges, and Kremer, Laurent
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- 2014
15. Correction to: TNF signaling and macrophages govern fin regeneration in zebrafish larvae
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Nguyen-Chi, Mai, Laplace-Builhé, Béryl, Travnickova, Jana, Luz-Crawford, Patricia, Tejedor, Gautier, Lutfalla, Georges, Kissa, Karima, Jorgensen, Christian, and Djouad, Farida
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- 2018
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16. Modelling 3D tumour microenvironment in a melanoma zebrafish model using live imaging technique: a tool to predict cancer fate
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Lorenzini, Francesca, Marines, Johanna, Kissa, Karima, and Fontenille, Laura
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This poster was presented by Ms. Lorenzini at the HoSC2021 "Hallmarks of Skin Cancer Conference", 29-30 November 2021. Abstract:Cutaneous melanoma occurrence has been increasing worldwide. Early diagnosis is fundamental to discover malignancies at early stages when they are characterized by low aggressiveness and low metastatic potential. To understand melanoma progression, the study of the tumour microenvironment (TME) dynamics has been becoming essential. Indeed, vessels play an important role as a pathway for cancer dissemination and oxygen-nutrient supply. Similarly, the recruitment and infiltration of immune cells in the tumour mass, such as macrophages, impact on cancer progression.Therefore, the 3D dynamic visualization of the TME appears as a predictive tool of cancer cell behaviour in terms of intravasation, invasiveness and metastasis. In this context, in vivo models are crucial to reliably reproduce the interactions between different cell types. In recent years, zebrafish embryos have emerged as a relevant tool in oncology. Among many advantages, its transparency allows to image and visualize in real time conserved cellular processes involved in tumour progression.
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- 2021
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17. Live imaging of emerging hematopoietic stem cells and early thymus colonization
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Kissa, Karima, Murayama, Emi, Zapata, Agustin, Cortés, Alfonso, Perret, Emmanuelle, Machu, Christophe, and Herbomel, Philippe
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- 2008
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18. Origins and unconventional behavior of neutrophils in developing zebrafish
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Le Guyader, Dorothée, Redd, Michael J., Colucci-Guyon, Emma, Murayama, Emi, Kissa, Karima, Briolat, Valérie, Mordelet, Elodie, Zapata, Agustin, Shinomiya, Hiroto, and Herbomel, Philippe
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- 2008
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19. Blood stem cells emerge from aortic endothelium by a novel type of cell transition
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Kissa, Karima and Herbomel, Philippe
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Endothelium -- Physiological aspects -- Research ,Hematopoietic stem cells -- Physiological aspects -- Research ,Mammals -- Physiological aspects ,Environmental issues ,Science and technology ,Zoology and wildlife conservation - Abstract
The ontogeny of haematopoietic stem cells (HSCs) during embryonic development is still highly debated, especially their possible lineage relationship to vascular endothelial cells (1,2). The first anatomical site from which cells with long-term HSC potential have been isolated is the aorta-gonad-mesonephros (AGM), more specifically the vicinity of the dorsal aortic floor (3). But although some authors have presented evidence that HSCs may arise directly from the aortic floor into the dorsal aortic lumen (4), others support the notion that HSCs first emerge within the underlying mesenchyme (5). Here we show by non-invasive, high-resolution imaging of live zebrafish embryos, that HSCs emerge directly from the aortic floor, through a stereotyped process that does not involve cell division but a strong bending then egress of single endothelial cells from the aortic ventral wall into the sub-aortic space, and their concomitant transformation into haematopoietic cells. The process is polarized not only in the dorso-ventral but also in the rostro-caudal versus medio-lateral direction, and depends on Runxl expression: in Runx1-deficient embryos, the exit events are initially similar, but much rarer, and abort into violent death of the exiting cell. These results demonstrate that the aortic floor is haemogenic and that HSCs emerge from it into the sub-aortic space, not by asymmetric cell division but through a new type of cell behaviour, which we call an endothelial haematopoietic transition., We (6,7) and others (8,9) have previously shown that in zebrafish embryos, the very thin space that initially separates the dorsal aorta (DA) from the underlying axial vein (AV) all [...]
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- 2010
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20. Bacteriophage Therapy for Staphylococcus Aureus Infections: A Review of Animal Models, Treatments, and Clinical Trials.
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Plumet, Lucile, Ahmad-Mansour, Nour, Dunyach-Remy, Catherine, Kissa, Karima, Sotto, Albert, Lavigne, Jean-Philippe, Costechareyre, Denis, and Molle, Virginie
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STAPHYLOCOCCUS aureus infections ,BACTERIOPHAGES ,TOXIC shock syndrome ,METHICILLIN-resistant staphylococcus aureus ,CLINICAL trials ,ANIMAL models in research - Abstract
Staphylococcus aureus (S. aureus) is a common and virulent human pathogen causing several serious illnesses including skin abscesses, wound infections, endocarditis, osteomyelitis, pneumonia, and toxic shock syndrome. Antibiotics were first introduced in the 1940s, leading to the belief that bacterial illnesses would be eradicated. However, microorganisms, including S. aureus , began to develop antibiotic resistance from the increased use and abuse of antibiotics. Antibiotic resistance is now one of the most serious threats to global public health. Bacteria like methicillin-resistant Staphylococcus aureus (MRSA) remain a major problem despite several efforts to find new antibiotics. New treatment approaches are required, with bacteriophage treatment, a non-antibiotic strategy to treat bacterial infections, showing particular promise. The ability of S. aureus to resist a wide range of antibiotics makes it an ideal candidate for phage therapy studies. Bacteriophages have a relatively restricted range of action, enabling them to target pathogenic bacteria. Their usage, usually in the form of a cocktail of bacteriophages, allows for more focused treatment while also overcoming the emergence of resistance. However, many obstacles remain, particularly in terms of their effects in vivo , necessitating the development of animal models to assess the bacteriophage efficiency. Here, we provide a review of the animal models, the various clinical case treatments, and clinical trials for S. aureus phage therapy. [ABSTRACT FROM AUTHOR]
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- 2022
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21. Tracing Hematopoietic Precursor Migration to Successive Hematopoietic Organs during Zebrafish Development
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Murayama, Emi, Kissa, Karima, Zapata, Agustin, Mordelet, Elodie, Briolat, Valérie, Lin, Hui-Feng, Handin, Robert I., and Herbomel, Philippe
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- 2006
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22. Imaging tissue remodeling during Endothelial to Hematopoietic Transition (EHT) in the zebrafish embryo
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Poullet, Nausicaa, Nicolas, Sandra, Lorman, Vladimir, Parmeggiani, Andrea, Kirchmaier, B, Schulte-Merker, Stephan, Kissa, Karima, Unité de Recherches Zootechniques (URZ), Institut National de la Recherche Agronomique (INRA), and European Molecular Biology Laboratory (EMBL). DEU.
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[SDV.GEN]Life Sciences [q-bio]/Genetics ,[SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics ,[SDV]Life Sciences [q-bio] - Abstract
International audience; Using the Danio Rerio embryo as a model, we demonstrated that hematopoietic stem cells (HSCs) emerge directly from the aorta floor through a process we named the endothelial haematopoietic transition (EHT). However, the molecular and cellular events driving this process still have to be addressed. In particular, we aim to characterize how tissue remodelling occurring during in EHT is linked to the underlying genetic reorganization. This interdisciplinary project capitalizes on the specific assets of the zebrafish model together with our expertise in 4D microscopy, 3D-image rendering and biophysical modeling. We focus on deciphering the sequence of events that govern EHT. Analysis of high resolution 4D confocal microscopy in collaboration with a team of biophysicists provides us with physico-mathematical models. This modelling integrates the role of the different mechanical forces applied to the vessel (shear stress, stretching, bending) as well as the cytoskeleton dynamics. The coordination of these key players allows the emergence of HSC without compromising the vessel integrity. Using new microscopy techniques, we also characterize the cellular environment of the AGM and assess the role of each cell component in the initiation and mobilization of the HSCs.This project will shed light on a critical biological process and may have direct applications in tissue engineering and regenerative therapies.
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- 2016
23. TNF signaling and macrophages govern fin regeneration in zebrafish larvae
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Nguyen-Chi, Mai, Laplace-Builhé, Béryl, Travnickova, Jana, Luz-Crawford, Patricia, Tejedor, Gautier, Lutfalla, Georges, Kissa, Karima, Jorgensen, Christian, Djouad, Farida, Cellules Souches, Plasticité Cellulaire, Médecine Régénératrice et Immunothérapies (IRMB), Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Dynamique des interactions membranaires normales et pathologiques (DIMNP), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université Montpellier 1 (UM1), Universidad de los Andes [Santiago] (UANDES), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), This work was supported by Inserm and grants from ‘Région Languedoc-Roussillon, Chercheur d’Avenir’ no. DGA3/DESR 2012/Q209, from the French National Research Agency ‘Zebraflam’ no. ANR-10-MIDI-009 and from the European Community’s Seventh Framework Program (FP7- PEOPLE-2011-ITN) under the Marie-Curie Initial Training Network FishForPharma (Grant Agreement No. PITN-GA-2011-289209). We thank the MRI facility for their assistance, J-P. Levraud (Institut Pasteur, France) for trans-shipping Tg(mpeg:Gal4, UAS:NTR-mCherry) line, A. Kawakami (Tokyo Institute of Technology, Japan) for junbl probe, M. Bagnat for tg(rcn3:gal4/UAS:dsRed) line and E. Lelièvre (Université de Montpellier, Montpellier) for providing support and advice during parabiosis experiments., ANR-10-MIDI-0009,ZebraFlam,Signaux et cellules de la réponse inflammatoire: suivi en temps réel chez un vertébré entier, le danio zébré(2010), European Project: 289209,EC:FP7:PEOPLE,FP7-PEOPLE-2011-ITN,FISHFORPHARMA(2012), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), LUTFALLA, Georges, MECANISMES INTEGRES DE L'INFLAMMATION - Signaux et cellules de la réponse inflammatoire: suivi en temps réel chez un vertébré entier, le danio zébré - - ZebraFlam2010 - ANR-10-MIDI-0009 - MI2 - VALID, and Training Network on Zebrafish Infection Models for Pharmaceutical Screens - FISHFORPHARMA - - EC:FP7:PEOPLE2012-01-01 - 2015-12-31 - 289209 - VALID
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animal structures ,Tumor Necrosis Factor-alpha ,Macrophage ,Macrophages ,fungi ,Correction ,Parabiosis ,Extremities ,Zebrafish Proteins ,[SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity ,Animals, Genetically Modified ,Receptors, Tumor Necrosis Factor, Type I ,Larva ,Animals ,Regeneration ,Original Article ,Blastema ,Fin regeneration ,Amputation ,Natural regeneration ,[SDV.IMM.II] Life Sciences [q-bio]/Immunology/Innate immunity ,Zebrafish ,Signal Transduction - Abstract
International audience; Macrophages are essential for appendage regeneration after amputation in regenerative species. The molecular mechanisms through which macrophages orchestrate blastema formation and regeneration are still unclear. Here, we use the genetically tractable and transparent zebrafish larvae to study the functions of polarized macrophage subsets during caudal fin regeneration. After caudal fin amputation, we show an early and transient accumulation of pro-inflammatory macrophages concomitant with the accumulation of non-inflammatory macrophages which, in contrast to pro-inflammatory macrophages, remain associated to the fin until the end of the regeneration. Chemical and genetic depletion of macrophages suggested that early recruited macrophages that express TNFα are critical for blastema formation. Combining parabiosis and morpholino knockdown strategies, we show that TNFα/TNFR1 signaling pathway is required for the fin regeneration. Our study reveals that TNFR1 has a necessary and direct role in blastema cell activation suggesting that macrophage subset balance provides the accurate TNFα signal to prime regeneration in zebrafish.
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- 2017
24. Identification of polarized macrophage subsets in zebrafish.
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Mai Nguyen-Chi, Laplace-Builhe, Béryl, Travnickova, Jana, Luz-Crawford, Patricia, Tejedor, Gautier, Quang Tien Phan, Duroux-Richard, Isabelle, Levraud, Jean-Pierre, Kissa, Karima, Lutfalla, Georges, Jorgensen, Christian, and Djouad, Farida
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MACROPHAGES ,ZEBRA danio - Abstract
The article presents the study that identifies the macrophages vital in the diverse abilities of zebrafish using the polarization process in France.
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- 2015
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25. Investigating Pathogenicity and Virulence of Staphylococcus pettenkoferi: An Emerging Pathogen.
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Ahmad-Mansour, Nour, Plumet, Lucile, Huc-Brandt, Sylvaine, Magnan, Chloé, Yahiaoui-Martinez, Alex, Kissa, Karima, Pantel, Alix, Lavigne, Jean-Philippe, and Molle, Virginie
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STAPHYLOCOCCUS ,DIABETIC foot ,GENOMICS ,PATHOGENIC bacteria ,STAPHYLOCOCCUS aureus ,PERITONEAL macrophages ,ZEBRA danio ,BRACHYDANIO - Abstract
Staphylococcus pettenkoferi is a coagulase-negative Staphylococcus identified in 2002 that has been implicated in human diseases as an opportunistic pathogenic bacterium. Its multiresistant character is becoming a major health problem, yet the pathogenicity of S. pettenkoferi is poorly characterized. In this study, the pathogenicity of a S. pettenkoferi clinical isolate from diabetic foot osteomyelitis was compared with a Staphylococcus aureus strain in various in vitro and in vivo experiments. Growth kinetics were compared against S. aureus, and bacteria survival was assessed in the RAW 264.7 murine macrophage cell line, the THP-1 human leukemia monocytic cell line, and the HaCaT human keratinocyte cell line. Ex vivo analysis was performed in whole blood survival assays and in vivo assays via the infection model of zebrafish embryos. Moreover, whole-genome analysis was performed. Our results show that S. pettenkoferi was able to survive in human blood, human keratinocytes, murine macrophages, and human macrophages. S. pettenkoferi demonstrated its virulence by causing substantial embryo mortality in the zebrafish model. Genomic analysis revealed virulence factors such as biofilm-encoding genes (e.g., icaABCD; rsbUVW) and regulator-encoding genes (e.g., agr, mgrA, sarA, saeS) well characterized in S. aureus. This study thus advances the knowledge of this under-investigated pathogen and validates the zebrafish infection model for this bacterium. [ABSTRACT FROM AUTHOR]
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- 2021
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26. Histopathological and cognitive defects induced by Nef in the brain.
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Mordelet, Elodie, Kissa, Karima, Cressant, Arnaud, Gray, Francoise, Ozden, Simona, Vidal, Catherine, Charneau, Pierre, and Granon, Sylvie
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HIV , *NEUROLOGICAL disorders , *PROTEINS , *AIDS , *LABORATORY rats , *MACROPHAGES , *NEUROTOXICOLOGY - Abstract
Complex mechanisms of human immunodeficiency virus type-1 (HIV-1) brain pathogenesis suggest the contribution of individual HIV-1 gene products. Among them, the Nef protein has been reported to harbor a major determinant of pathogenicity in AIDS-like disease. The goal of the present study was to determine whether Nef protein expressed in vivo by primary macrophages could induce a brain toxicity also affecting the behavior of the rat. To achieve this goal we grafted Nef-transduced macrophages into the rat hippocampus. Two months post-transplantation, we observed that Nef induces monocyte/macrophage recruitment, expression of TNF-α, and astrogliosis. No apoptotic event was detected. We further demonstrated that Nef neurotoxicity is associated with cognitive deficits.--Mordelet, E., Kissa, K., Cressant, A., Gray, F., Ozden, S., Vidal, C., Charneau, P., Granon, S. Histopathological and cognitive defects induced by Nef in the brain. [ABSTRACT FROM AUTHOR]
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- 2004
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27. In Vivo Neuronal Tracing with GFP-TTC Gene Delivery
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Kissa, Karima, Mordelet, Elodie, Soudais, Claire, Kremer, Eric J., Demeneix, Barbara A., Brûlet, Philippe, and Coen, Laurent
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TETANUS toxin , *CENTRAL nervous system - Abstract
The retrograde transport and transynaptic transfer properties of the nontoxic tetanus toxin C-fragment (TTC) can be used to visualize specific neural pathways or to deliver biomolecules in the central nervous system (CNS). Here we tested different delivery techniques to explore the potential use of a new GFP-TTC fusion construct for use as a genetic tracer in vivo. Plasmids encoding GFP-TTC were targeted to brain regions using intracerebral grafted transfected cells or adenoviral transduction. Transport was monitored using GFP fluorescence. We show that following GFP-TTC synthesis in grafted transfected cells, the TTC fragment alone, with no signal peptide, is necessary and sufficient to provide secretion and uptake of the fusion protein into neighboring neurons around the injection site. Using an adenoviral vector to express the fusion protein into brain neurons, we show that transduced neurons can deliver the fusion protein specifically into connected neurons, demonstrating that synaptic transfer in the CNS can be visualized with GFP-TTC. [Copyright &y& Elsevier]
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- 2002
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28. Generating parabiotic zebrafish embryos for cell migration and homing studies.
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Demy, Doris Lou, Ranta, Zachary, Giorgi, Jean-Michel, Gonzalez, Magali, Herbomel, Philippe, and Kissa, Karima
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CYTOLOGICAL research ,PARABIOSIS ,CELL migration ,ZEBRA danio ,EMBRYOS ,BLASTULA - Abstract
Parabiosis, the surgical generation of conjoined organisms sharing a common bloodstream, has been a powerful tool for studying hematopoietic cell migration and interaction with stromal niches in rodent and avian systems. We describe a technique to generate parabiotic zebrafish embryos based on blastula fusion. This procedure permits the in vivo visualization of hematopoietic cell migration and homing to niches and peripheral tissues in zebrafish parabiotes of different genetic backgrounds. [ABSTRACT FROM AUTHOR]
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- 2013
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29. Primitive macrophages control HSPC mobilisation and definitive haematopoiesis.
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Kissa, Karima
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MACROPHAGES , *HEMATOPOIETIC stem cells , *PROGENITOR cells , *BONE marrow , *HEMATOPOIETIC system , *HEMATOPOIESIS , *HEMATOLOGY - Published
- 2015
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30. Primitive macrophages control HSPC mobilization and definitive haematopoiesis.
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Travnickova, Jana, Tran Chau, Vanessa, Julien, Emmanuelle, Mateos-Langerak, Julio, Gonzalez, Catherine, Lelièvre, Etienne, Lutfalla, Georges, Tavian, Manuela, and Kissa, Karima
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- 2015
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31. The zebrafish embryo model: unveiling its potential for investigating phage therapy against methicillin-resistant Staphylococcus aureus infection.
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Plumet L, Magnan C, Ahmad-Mansour N, Sotto A, Lavigne J-P, Costechareyre D, Kissa K, and Molle V
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- Animals, Disease Models, Animal, Embryo, Nonmammalian microbiology, Microbial Sensitivity Tests, Zebrafish microbiology, Methicillin-Resistant Staphylococcus aureus drug effects, Methicillin-Resistant Staphylococcus aureus virology, Phage Therapy methods, Vancomycin pharmacology, Vancomycin therapeutic use, Staphylococcal Infections therapy, Staphylococcal Infections microbiology, Staphylococcal Infections drug therapy, Anti-Bacterial Agents pharmacology, Anti-Bacterial Agents therapeutic use
- Abstract
Staphylococcus aureus is a pathogenic bacterium responsible for a broad spectrum of infections, including cutaneous, respiratory, osteoarticular, and systemic infections. It poses a significant clinical challenge due to its ability to develop antibiotic resistance. This resistance limits therapeutic options, increases the risk of severe complications, and underscores the urgent need for new strategies to address this threat, including the investigation of treatments complementary to antibiotics. The evaluation of novel antimicrobial agents often employs animal models, with the zebrafish embryo model being particularly interesting for studying host-pathogen interactions, establishing itself as a crucial tool in this field. For the first time, this study presents a zebrafish embryo model for the in vivo assessment of bacteriophage efficacy against S. aureus infection. A localized infection was induced by microinjecting either methicillin-resistant S. aureus (MRSA) or methicillin-susceptible S. aureus (MSSA). Subsequent treatments involved administering either bacteriophage, vancomycin (the reference antibiotic for MRSA), or a combination of both via the same route to explore potential synergistic effects. Our findings indicate that the bacteriophage was as effective as vancomycin in enhancing survival rates, whether used alone or in combination. Moreover, bacteriophage treatment appears to be even more effective in reducing the bacterial load in S. aureus -infected embryos post-treatment than the antibiotic. Our study validates the use of the zebrafish embryo model and highlights its potential as a valuable tool in assessing bacteriophage efficacy treatments in vivo ., Competing Interests: The authors declare no conflict of interest.
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- 2024
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32. Modelling 3D Tumour Microenvironment In Vivo: A Tool to Predict Cancer Fate.
- Author
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Marines J, Lorenzini F, Kissa K, and Fontenille L
- Abstract
Recently, many studies demonstrated the fundamental role of the tumour microenvironment (TME) in cancer progression. Here, we describe a method to visualize in 3D the behaviour of tumours in zebrafish embryos. We highlight two major actors of the TME, macrophages and vessels. This valuable tool is transposable to Patient-Derived Xenograft imaging in order to predict the fate of malignant tumours according to the dynamics of their TME.
- Published
- 2023
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33. Deficiency in hereditary hemorrhagic telangiectasia-associated Endoglin elicits hypoxia-driven heart failure in zebrafish.
- Author
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Lelièvre E, Bureau C, Bordat Y, Frétaud M, Langevin C, Jopling C, and Kissa K
- Subjects
- Animals, Endoglin genetics, Endoglin metabolism, Zebrafish, Endothelial Cells metabolism, Activin Receptors, Type II genetics, Telangiectasia, Hereditary Hemorrhagic complications, Telangiectasia, Hereditary Hemorrhagic genetics, Heart Failure metabolism
- Abstract
Hereditary hemorrhagic telangiectasia (HHT) is a rare genetic disease caused by mutations affecting components of bone morphogenetic protein (BMP)/transforming growth factor-β (TGF-β) signaling in endothelial cells. This disorder is characterized by arteriovenous malformations that are prone to rupture, and the ensuing hemorrhages are responsible for iron-deficiency anemia. Along with activin receptor-like kinase (ALK1), mutations in endoglin are associated with the vast majority of HHT cases. In this study, we characterized the zebrafish endoglin locus and demonstrated that it produces two phylogenetically conserved protein isoforms. Functional analysis of a CRISPR/Cas9 zebrafish endoglin mutant revealed that Endoglin deficiency is lethal during the course from juvenile stage to adulthood. Endoglin-deficient zebrafish develop cardiomegaly, resulting in heart failure and hypochromic anemia, which both stem from chronic hypoxia. endoglin mutant zebrafish display structural alterations of the developing gills and underlying vascular network that coincide with hypoxia. Finally, phenylhydrazine treatment demonstrated that lowering hematocrit/blood viscosity alleviates heart failure and enhances the survival of Endoglin-deficient fish. Overall, our data link Endoglin deficiency to heart failure and establish zebrafish as a valuable HHT model., Competing Interests: Competing interests The authors declare no competing or financial interests., (© 2023. Published by The Company of Biologists Ltd.)
- Published
- 2023
- Full Text
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34. DNA damage repair kinase DNA-PK and cGAS synergize to induce cancer-related inflammation in glioblastoma.
- Author
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Taffoni C, Marines J, Chamma H, Guha S, Saccas M, Bouzid A, Valadao AC, Maghe C, Jardine J, Park MK, Polak K, De Martino M, Vanpouille-Box C, Del Rio M, Gongora C, Gavard J, Bidère N, Song MS, Pineau D, Hugnot JP, Kissa K, Fontenille L, Blanchet FP, Vila IK, and Laguette N
- Subjects
- Humans, Carcinogenesis, DNA metabolism, DNA Damage, DNA Repair, Immunity, Innate, Inflammation, Tumor Microenvironment, Glioblastoma genetics, Nucleotidyltransferases metabolism, DNA-Activated Protein Kinase metabolism
- Abstract
Cytosolic DNA promotes inflammatory responses upon detection by the cyclic GMP-AMP (cGAMP) synthase (cGAS). It has been suggested that cGAS downregulation is an immune escape strategy harnessed by tumor cells. Here, we used glioblastoma cells that show undetectable cGAS levels to address if alternative DNA detection pathways can promote pro-inflammatory signaling. We show that the DNA-PK DNA repair complex (i) drives cGAS-independent IRF3-mediated type I Interferon responses and (ii) that its catalytic activity is required for cGAS-dependent cGAMP production and optimal downstream signaling. We further show that the cooperation between DNA-PK and cGAS favors the expression of chemokines that promote macrophage recruitment in the tumor microenvironment in a glioblastoma model, a process that impairs early tumorigenesis but correlates with poor outcome in glioblastoma patients. Thus, our study supports that cGAS-dependent signaling is acquired during tumorigenesis and that cGAS and DNA-PK activities should be analyzed concertedly to predict the impact of strategies aiming to boost tumor immunogenicity., (© 2022 The Authors. Published under the terms of the CC BY 4.0 license.)
- Published
- 2023
- Full Text
- View/download PDF
35. Comment on: 'Homozygous knockout of the piezo1 gene in the zebrafish is not associated with anemia'.
- Author
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Faucherre A, Kissa K, Nargeot J, Mangoni ME, and Jopling C
- Subjects
- Anemia, Animals, Homozygote, Humans, Anemia, Hemolytic, Congenital genetics, Zebrafish
- Published
- 2016
- Full Text
- View/download PDF
36. Identification of polarized macrophage subsets in zebrafish.
- Author
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Nguyen-Chi M, Laplace-Builhe B, Travnickova J, Luz-Crawford P, Tejedor G, Phan QT, Duroux-Richard I, Levraud JP, Kissa K, Lutfalla G, Jorgensen C, and Djouad F
- Subjects
- Animals, Animals, Genetically Modified, Escherichia coli Infections immunology, Flow Cytometry, Gene Expression Profiling, Genes, Reporter, Microscopy, Confocal, Molecular Sequence Data, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, DNA, Tumor Necrosis Factor-alpha biosynthesis, Wounds and Injuries immunology, Macrophages classification, Macrophages immunology, Zebrafish immunology
- Abstract
While the mammalian macrophage phenotypes have been intensively studied in vitro, the dynamic of their phenotypic polarization has never been investigated in live vertebrates. We used the zebrafish as a live model to identify and trail macrophage subtypes. We generated a transgenic line whose macrophages expressing tumour necrosis factor alpha (tnfa), a key feature of classically activated (M1) macrophages, express fluorescent proteins Tg(mpeg1:mCherryF/tnfa:eGFP-F). Using 4D-confocal microscopy, we showed that both aseptic wounding and Escherichia coli inoculation triggered macrophage recruitment, some of which started to express tnfa. RT-qPCR on Fluorescence Activated Cell Sorting (FACS)-sorted tnfa(+) and tnfa(-) macrophages showed that they, respectively, expressed M1 and alternatively activated (M2) mammalian markers. Fate tracing of tnfa(+) macrophages during the time-course of inflammation demonstrated that pro-inflammatory macrophages converted into M2-like phenotype during the resolution step. Our results reveal the diversity and plasticity of zebrafish macrophage subsets and underline the similarities with mammalian macrophages proposing a new system to study macrophage functional dynamic.
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- 2015
- Full Text
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37. Piezo1 plays a role in erythrocyte volume homeostasis.
- Author
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Faucherre A, Kissa K, Nargeot J, Mangoni ME, and Jopling C
- Subjects
- Animals, Erythropoiesis genetics, Gene Expression Regulation, Developmental, Gene Knockdown Techniques, Ion Channels metabolism, Zebrafish embryology, Zebrafish Proteins metabolism, Erythrocyte Volume genetics, Homeostasis genetics, Ion Channels genetics, Zebrafish blood, Zebrafish genetics, Zebrafish Proteins genetics
- Abstract
Mechanosensitivity is an inherent property of virtually all cell types, allowing them to sense and respond to physical environmental stimuli. Stretch-activated ion channels represent a class of mechanosensitive proteins which allow cells to respond rapidly to changes in membrane tension; however their identity has remained elusive. The piezo genes have recently been identified as a family of stretch-activated mechanosensitive ion channels. We set out to determine the role of piezo1 during zebrafish development. Here we report that morpholino-mediated knockdown of piezo1 impairs erythrocyte survival without affecting hematopoiesis or differentiation. Our results demonstrate that piezo1 is involved in erythrocyte volume homeostasis, disruption of which results in swelling/lysis of red blood cells and consequent anemia.
- Published
- 2014
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38. Studying cell behavior in whole zebrafish embryos by confocal live imaging: application to hematopoietic stem cells.
- Author
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Renaud O, Herbomel P, and Kissa K
- Subjects
- Animals, Animals, Genetically Modified, Zebrafish genetics, Embryo, Nonmammalian cytology, Hematopoietic Stem Cells cytology, Microscopy, Confocal methods, Zebrafish embryology
- Abstract
Confocal live imaging is a key tool for studying cell behavior in the whole zebrafish embryo. Here we provide a detailed protocol that is adaptable for imaging any progenitor cell behavior in live zebrafish embryos. As an example, we imaged the emergence of the first hematopoietic stem cells from the aorta. We discuss the importance of selecting the appropriate zebrafish transgenic line as well as methods for immobilization of embryos to be imaged. In addition, we highlight the confocal microscopy acquisition parameters required for stem cell imaging and the software tools we used to analyze 4D movies. The whole protocol takes 2 h 15 min and allows confocal live imaging from a few hours to several days.
- Published
- 2011
- Full Text
- View/download PDF
39. Real-time observation of listeria monocytogenes-phagocyte interactions in living zebrafish larvae.
- Author
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Levraud JP, Disson O, Kissa K, Bonne I, Cossart P, Herbomel P, and Lecuit M
- Subjects
- Animals, Bacterial Proteins physiology, Bacterial Toxins, Cadherins physiology, Heat-Shock Proteins physiology, Hemolysin Proteins physiology, Larva microbiology, Macrophages microbiology, Membrane Proteins physiology, Neutrophils microbiology, Virulence Factors physiology, Zebrafish embryology, Listeria monocytogenes pathogenicity, Phagocytes microbiology, Zebrafish microbiology
- Abstract
The zebrafish, Danio rerio, has become a popular vertebrate model for the study of infections, mainly because of its excellent optical accessibility at the embryonic and larval stages, when the innate immune system is already effective. We have thus tested the susceptibility of zebrafish larvae to the human pathogen Listeria monocytogenes, a gram-positive, facultative, intracellular bacterium that is known to survive and multiply in professional phagocytes and that causes fatal meningitis and abortions. Intravenous injection of early zebrafish larvae resulted in a progressive and ultimately fatal infection. Blood-borne L. monocytogenes bacteria were quickly trapped and engulfed by macrophages, an event that, for the first time, could be captured in vivo and in real time. Granulocytes also participated in the innate immune response. As in mammals, bacteria could escape the macrophage phagosome in a listeriolysin-dependent manner and accessed the cytosol; this event was critical for bacterial virulence, as listeriolysin-deficient bacteria were completely avirulent. Actin comet tails and protrusions were observed, suggesting cell-to-cell spread; these phenomena also played a role in virulence in zebrafish larvae, as actA-deficient bacteria were attenuated. These results demonstrate the relevance of the genetically tractable and optically accessible zebrafish model for the study of L. monocytogenes pathogenesis and particularly for the dissection of its interactions with phagocytes in vivo, a key factor of L. monocytogenes virulence.
- Published
- 2009
- Full Text
- View/download PDF
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