Modelling 3D tumour microenvironment in a melanoma zebrafish model using live imaging technique: a tool to predict cancer fate

This poster was presented by Ms. Lorenzini at the HoSC2021 "Hallmarks of Skin Cancer Conference", 29-30 November 2021. Abstract:Cutaneous melanoma occurrence has been increasing worldwide. Early diagnosis is fundamental to discover malignancies at early stages when they are characterized by low aggressiveness and low metastatic potential. To understand melanoma progression, the study of the tumour microenvironment (TME) dynamics has been becoming essential. Indeed, vessels play an important role as a pathway for cancer dissemination and oxygen-nutrient supply. Similarly, the recruitment and infiltration of immune cells in the tumour mass, such as macrophages, impact on cancer progression.Therefore, the 3D dynamic visualization of the TME appears as a predictive tool of cancer cell behaviour in terms of intravasation, invasiveness and metastasis. In this context, in vivo models are crucial to reliably reproduce the interactions between different cell types. In recent years, zebrafish embryos have emerged as a relevant tool in oncology. Among many advantages, its transparency allows to image and visualize in real time conserved cellular processes involved in tumour progression.