29 results on '"He, Xinjia"'
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2. CdSe quantum dots-decorated ZnIn2S4 nanosheets for “signal-on” photoelectrochemical aptasensing of ATP by integrating exciton energy transfer with exciton-plasmon coupling
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He, Xinjia, Zhao, Xiao, Deng, Wenfang, Tan, Yueming, and Xie, Qingji
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- 2021
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3. Cobalt-doped tungsten trioxide nanorods decorated with Au nanoparticles for ultrasensitive photoelectrochemical detection of aflatoxin B1 based on aptamer structure switch
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He, Xinjia, Ying, Ying, Zhao, Xiao, Deng, Wenfang, Tan, Yueming, and Xie, Qingji
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- 2021
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4. Charge Photoaccumulation in Covalent Polymer Networks for Boosting Photocatalytic Nitrate Reduction to Ammonia.
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He, Xinjia, Wen, Yingke, Fang, Yanjie, Li, Mengjie, and Shan, Bing
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DENITRIFICATION , *PHOTOREDUCTION , *CHEMICAL reduction , *COPPER catalysts , *CHARGE exchange , *PHOTOINDUCED electron transfer , *ELECTROCATALYSIS - Abstract
In the design of photoelectrocatalytic cells, a key element is effective photogeneration of electron‐hole pairs to drive redox activation of catalysts. Despite recent progress in photoelectrocatalysis, experimental realization of a high‐performance photocathode for multi‐electron reduction of chemicals, such as nitrate reduction to ammonia, has remained a challenge due to difficulty in obtaining efficient electrode configurations for extraction of high‐throughput electrons from absorbed photons. This work describes a new design for catalytic photoelectrodes using chromophore assembly‐functionalized covalent networks for boosting eight‐electron reduction of nitrate to ammonia. Upon sunlight irradiation, the photoelectrode stores a mass of reducing equivalents at the photoexcited chromophore assembly for multielectron reduction of a copper catalyst, enabling efficient nitrate reduction to ammonia. By introducing the new photoelectrode structure, it is demonstrated that the electronic interplay between charge photo‐accumulating assembly and multi‐electron redox catalysts can be optimized to achieve proper balance between electron transfer dynamics and thermodynamic output of photoelectrocatalytic systems. [ABSTRACT FROM AUTHOR]
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- 2024
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5. Astaxanthin Augmented the Anti‐Hepatocellular Carcinoma Efficacy of Sorafenib Through the Inhibition of the JAK2/STAT3 Signaling Pathway and Mitigation of Hypoxia within the Tumor Microenvironment.
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Ren, Pengfei, Tang, Qingjuan, He, Xinjia, Xu, Jie, Wang, Yuming, and Xue, Changhu
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- 2024
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6. Caspase-dependent and caspase-independent induction of apoptosis in breast cancer by fucoidan via the PI3K/AKT/GSK3β pathway in vivo and in vitro
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Xue, Meilan, Ji, Xinqiang, Xue, Chuanxing, Liang, Hui, Ge, Yinlin, He, Xinjia, Zhang, Li, Bian, Kang, and Zhang, Lichen
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- 2017
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7. The dietary polysaccharide from Ommastrephes bartrami prevents chemotherapeutic mucositis by promoting the gene expression of antimicrobial peptides in Paneth cells
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Zuo, Tao, He, Xinjia, Cao, Lu, Xue, Changhu, and Tang, Qing-Juan
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- 2015
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8. Hyaluronidase Enzyme-responsive Targeted Nanoparticles for Effective Delivery of 5-Fluorouracil in Colon Cancer
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Jiang, Haiping, Shi, Xinyan, Yu, Xiaoyun, He, Xinjia, An, Yongheng, and Lu, Haijun
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- 2018
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9. Radiobiological effect of abdominal X-ray hypo-fraction irradiation on Wistar rats liver
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Qi, Peng, An, Yongheng, Yu, Hongsheng, and He, Xinjia
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- 2012
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10. Effects of low dose pre-irradiation on the toxicity of cyclophosphamide
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Yu, Hongsheng, Yu, Li, Song, Aiqin, Liu, Zimin, Zhang, Yeling, and He, Xinjia
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- 2011
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11. Type IIA topoisomerase (TOP2A) triggers epithelial-mesenchymal transition and facilitates HCC progression by regulating Snail expression.
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Dong, Yinying, Sun, Xiangyin, Zhang, Kong, He, Xinjia, Zhang, Qian, Song, Hao, Xu, Mingjin, Lu, Haijun, and Ren, Ruimei
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- 2021
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12. piR-hsa-211106 Inhibits the Progression of Lung Adenocarcinoma Through Pyruvate Carboxylase and Enhances Chemotherapy Sensitivity.
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Liu, Yongmei, Dong, Yanhan, He, Xinjia, Gong, Anjing, Gao, Jinning, Hao, Xiaodan, Wang, Shuai, Fan, Yuqiao, Wang, Zibo, Li, Meng, and Xu, Wenhua
- Subjects
PYRUVATE carboxylase ,INHIBITION of cellular proliferation ,CANCER chemotherapy ,ADENOCARCINOMA ,LUNGS - Abstract
Although the importance of PIWI-interacting RNAs (piRNAs) in cancer has recently been recognized, studies on the role and functional mechanism of piRNAs in lung adenocarcinoma (LUAD) development and progression are limited. In this study, we identified 10 differently expressed piRNAs in LUAD tissues compared to normal tissues, among which, piR-hsa-211106 expression levels were downregulated in LUAD tissues and cell lines. Furthermore, the effects of piR-hsa-211106 on the malignant phenotypes and chemosensitivity of LUAD cells were detected by gain- and loss-of-function analyses in vitro and in vivo , which showed that piR-hsa-211106 inhibited LUAD cell proliferation, tumor growth, and migration, but promoted apoptosis. Moreover, our finding indicated that piR-hsa-211106 is a potential therapeutic target that synergistically imparts anticancer effects with a chemotherapeutic agent for LUAD-cisplatin. Further mechanistic investigation indicated that piR-hsa-211106 could bind to pyruvate carboxylase (PC) by RNA pull down and RNA immunoprecipitation assays and inhibited PC mRNA and protein expression. Our study demonstrates that piR-hsa-211106 inhibits LUAD progression by hindering the expression and function of PC and enhances chemotherapy sensitivity, suggesting that piR-hsa-211106 is a novel diagnostic and therapeutic target for LUAD. [ABSTRACT FROM AUTHOR]
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- 2021
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13. Protective effect of trimetazidine in radiation-induced cardiac fibrosis in mice.
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Zhang, Jinmeng, He, Xinjia, Bai, Xinya, Sun, Yang, Jiang, Peng, Wang, Xiang, Li, Wei, and Zhang, Yuliang
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RADIOTHERAPY ,RADIOTHERAPY complications ,HEART fibrosis ,CONNECTIVE tissue growth factor ,TRANSFORMING growth factors ,GENE expression - Abstract
Radiation-induced heart damage is a serious side effect caused by radiotherapy, especially during the treatment of cancer near the chest. Trimetazidine is effective at reducing inflammation in the heart, but how it affects radiation-induced cardiac fibrosis (RICF) is unknown. To investigate the potential effect and molecular mechanism, we designed this project with a C57BL6 male mouse model supposing trimetazidine could inhibit RICF in mice. During the experiment, mice were randomly divided into six groups including a control group (Con), radiation-damaged model group (Mod) and four experimental groups receiving low-dose (10 mg/kg/day) or high-dose (20 mg/kg/day) trimetazidine before or after radiation treatment. Apart from the control group, all mice chests were exposed to 6 MV X-rays at a single dose of 20 Gy to induce RICF, and tissue analysis was done at 8 weeks after irradiation. Fibroblast or interstitial tissues and cardiac fibrosis-like characteristics were determined using haematoxylin and eosin and Masson staining, which can be used to assess myocardial fibrosis. Immunohistochemical analysis and RT-PCR were used to determine gene expression and study the molecular mechanism. As a result, this study suggests that trimetazidine inhibits RICF by reducing gene expression related to myocyte apoptosis and fibrosis formation, i.e. connective tissue growth factor (CTGF), transforming growth factor (TGF)-β1, smad2 and smad3. In conclusion, by regulating the CTGF/TGF-β1/Smad pathway, trimetazidine could be a prospective drug for clinical treatment of RICF. [ABSTRACT FROM AUTHOR]
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- 2020
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14. TRIM23 overexpression is a poor prognostic factor and contributes to carcinogenesis in colorectal cancer.
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Han, Yudong, Tan, Ye, Zhao, Yuanyuan, Zhang, Yongchun, He, Xinjia, Yu, Li, Jiang, Haiping, Lu, Haijun, and Tian, Haiying
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COLORECTAL cancer ,CANCER prognosis ,CELL cycle ,CELL proliferation ,REGRESSION analysis ,LYMPH nodes ,ONCOGENES ,UBIQUITINATION - Abstract
The tripartite motif (TRIM) family proteins play a great role in carcinogenesis. However, the expression pattern, prognostic value and biological functions of tripartite motif containing 23 (TRIM23) in colorectal cancer (CRC) are poorly understood. Here, we found that TRIM23 is up‐regulated and associated with tumour size, lymph node metastasis, American Joint Committee on Cancer (AJCC) stage and poor prognosis in CRC. Multivariate Cox regression analyses revealed that TRIM23 overexpression could be identified as an independent prognostic factor for CRC. TRIM23 could promote the proliferation of CRC cell in vitro and in vivo; additionally, TRIM23 depletion induced G1-phase arrest. Gene set enrichment analysis (GSEA) revealed that P53 and cell cycle signalling pathway‐related genes were enriched in patients with high TRIM23 expression levels. We show in this study that TRIM23 physically binds to P53 and enhances the ubiquitination of P53, thereby promoting tumour proliferation. Thus, our data indicated that TRIM23 acts as an oncogene in colorectal carcinogenesis and may provide a novel therapeutic target for CRC management. [ABSTRACT FROM AUTHOR]
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- 2020
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15. RBBP7 is a prognostic biomarker in patients with esophageal squamous cell carcinoma.
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Yu, Ning, Zhang, Peng, Wang, Li, He, Xinjia, Yang, Shanshan, and Lu, Haijun
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RETINOBLASTOMA gene ,RETINOBLASTOMA protein ,CARRIER proteins ,BIOLOGICAL transport ,PROTEIN binding - Abstract
Retinoblastoma-binding protein 7 (RBBP7) is an important component of several complexes that regulate chromatin metabolism. It is overexpressed in certain cancer types and serves conflicting roles in tumor progression. In the present study, the expression and roles of RBBP7 were explored in esophageal squamous cell carcinoma (ESCC). Immunohistochemical staining was used to detect RBBP7 expression in ESCC tissues. The mRNA sequencing profiles from the Cancer Genome Atlas and Genotype-Tissue Expression databases were mined to analyze the mRNA expression of RBBP7 in tissues. Proliferation, clone formation, apoptosis and Transwell invasion/migration assays were performed to explore the roles of RBBP7 in ESCC. RBBP7 was highly expressed in ESCC tissues. The protein and mRNA expression levels of RBBP7 were significantly elevated in tumor tissues compared with paired adjacent normal tissues. RBBP7 overexpression was associated with a poor overall survival in patients with ESCC. Furthermore, higher RBBP7 expression was significantly correlated with poor tumor differentiation, advanced regional lymph node involvement, and pathological TNM staging. Knockdown of RBBP7 in ESCC cells did not affect tumor apoptosis or tumor growth. However, the overexpression of RBBP7 significantly enhanced the invasion and migration of ESCC cells, whereas the knockdown of RBBP7 resulted in significantly decreased invasion and migration. The present study indicated that RBBP7 is a novel biomarker and prognosticator for patients with ESCC. Furthermore, RBBP7 serves crucial roles in promoting ESCC invasion and migration. [ABSTRACT FROM AUTHOR]
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- 2018
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16. The Protective and Immunomodulatory Effects of Fucoidan Against 7,12-Dimethyl benz[a]anthracene-Induced Experimental Mammary Carcinogenesis Through the PD1/PDL1 Signaling Pathway in Rats.
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Xue, Meilan, Liang, Hui, Tang, Qingjuan, Xue, Chuanxing, He, Xinjia, Zhang, Li, Zhang, Zheng, Liang, Zhengyan, Bian, Kang, Zhang, Lichen, and Li, Zhuxin
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BREAST tumor prevention ,ENZYME metabolism ,ALGAE ,ANIMAL experimentation ,BREAST tumors ,CELLULAR signal transduction ,FLOW cytometry ,INTERFERONS ,INTERLEUKINS ,KILLER cells ,MEDICINAL plants ,POLYSACCHARIDES ,RATS ,T cells ,TRANSFORMING growth factors-beta ,IN vivo studies ,BLOOD - Abstract
Fucoidan is a sulfated polysaccharide that is extracted from brown algae seaweed. This study was designed to evaluate the protective and immunomodulatory effects of dietary fucoidan on 7,12-dimethyl benz[a]anthracene (DMBA)-induced experimental mammary carcinogenesis in rats. Sixty Sprague-Dawley rats were randomly assigned to four equal groups: the control group (control group), the cancer model group (model group), and the F1 and F2 groups, which were fed fucoidan at concentrations of 200 and 400 mg/kg·body weight, respectively. We found that fucoidan treatment decreased the tumor incidence and mean tumor weight and prolonged the tumor latency. Flow cytometric analyses revealed that the number of blood natural killer cells was higher after fucoidan treatment and that the proportions of CD4 and CD8 T cells were also increased. The serum levels of interleukin (IL)-6, IL-12p40, and interferon (IFN)-γ were higher in the rats treated with fucoidan compared to those of model rats. Moreover, the percentage of CD3+ Foxp3+ regulatory T cells in the blood and the levels of IL-10 and transforming growth factor β in the serum were lower in the rats treated with fucoidan. Furthermore, fucoidan treatment decreased the expression of Foxp3 and programmed cell death 1 ligand 1 (PDL1) in tumor tissues. The levels of p-phosphatidyl inositol kinase 3 and p-AKT in tumor tissues were also lower than those of model rats. These results suggest that a fucoidan-supplemented diet can inhibit DMBA-induced tumors in rats. This study provides experimental evidence toward elucidating the immune enhancement induced by fucoidan through the programmed cell death 1/PDL1 signaling pathway. The immunomodulatory effect is one of the possible mechanisms of the protective effect of fucoidan against mammary carcinogenesis. [ABSTRACT FROM PUBLISHER]
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- 2017
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17. Manipulating Photoconduction in Supramolecular Networks for Solar-Driven Nitrate Conversion to Ammonia and Oxygen.
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Hong F, Su X, Fang Y, He X, and Shan B
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For photoelectrodes to be used in practical catalytic applications, challenges exist in achieving the efficient production and transport of photogenerated charge-separated states. Analogous concepts in traditional inorganic photoelectrodes can be applied to their organic-polymer counterparts with improved charge-separation efficiencies. In this work, we develop photoconductive organic networks to form a high-performance photoelectrode for NO
3 - reduction to NH3 . In the integrated network, interfaces between the organic electron-donating photoconductor and electron-accepting catalyst can generate charge carriers efficiently upon illumination, leading to enhanced charge separation for photoelectrocatalysis. The photoelectrode network is capable of converting NO3 - to NH3 at an external quantum efficiency of 13%. By coupling with a BiVO4 photoanode in tandem, the system reduces NO3 - to NH3 and oxidizes H2 O to O2 simultaneously at Faradaic efficiencies of 95-98% with sustained photocurrents and production yields. Investigation of the photoconductive network by steady-state/time-resolved spectroscopies reveals the efficient generation and transport of free charge carriers in the photoelectrode, providing a basis for high photoelectrocatalytic performances.- Published
- 2024
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18. Photoelectrocatalytic CO 2 Reduction to Methanol by Molecular Self-Assemblies Confined in Covalent Polymer Networks.
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Fang Y, Gao Y, Wen Y, He X, Meyer TJ, and Shan B
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Inspired by the porous structures of photosynthetic organelles, we report here a new type of photoelectrode based on a standalone macroporous conjugated polymer network (MCN) that converts sunlight into high-energy electrons for CO
2 reduction to CH3 OH. The MCN provides supramolecular cavities with sufficient functional groups that control the structures of photocatalytic assemblies, which circumvents the geometric limitations of traditional inorganic counterparts. Stabilized interfacial contact between MCN and photocatalysts is achieved by strong chemical linkages throughout the network. Solar irradiation of MCN with a cobalt-based catalyst generates highly reducing electrons for the reduction of CO2 to CH3 OH at a conversion efficiency of 70%. Production of CH3 OH sustains for at least 100 h, with a small decrease in yield rates. Scaling up the photoelectrode from 1 to 100 cm2 results in photocurrent generation stabilized at 0.25 A and continuous CH3 OH production at a conversion efficiency of 85%, demonstrating the scalability and high performances.- Published
- 2024
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19. Gastric-type endocervical adenocarcinoma: a case report and literature review.
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Wen X, Yu L, Liu X, He X, Zhao Y, and Li G
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Gastric-type endocervical adenocarcinoma (G-EAC) represents a rare variant of cervical mucinous adenocarcinoma that is typically unrelated to human papillomavirus (HPV) infection. G-EAC exhibits highly atypical clinical presentations and characteristics, and aggressive biological behavior often leads to challenges in timely diagnosis. Here, we present a case study involving a 74-year-old Chinese woman who experienced urinary incontinence for one month. Biopsy pathology confirmed the diagnosis of G-EAC, revealing stage IVa by imaging examinations. The patient subsequently underwent three cycles of chemotherapy, followed by adjuvant radiotherapy and surgical excision of residual tumor foci. This comprehensive treatment approach yielded a favorable survival outcome. For patients with advanced G-EAC, a multimodal therapeutic approach holds promise and warrants further exploration., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Wen, Yu, Liu, He, Zhao and Li.)
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- 2024
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20. Five-year disease-free survival of Epstein-Barr virus-associated locoregionally advanced undifferentiated nasopharyngeal carcinoma patients treated with chemo-radiotherapy: a case report.
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Wang N, Wang H, Jiang H, Bi Y, Zhao Y, He X, Cao F, and Yuan Y
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- Adult, Chemoradiotherapy, Disease-Free Survival, Herpesvirus 4, Human, Humans, Male, Nasopharyngeal Carcinoma diagnosis, Nasopharyngeal Carcinoma drug therapy, Epstein-Barr Virus Infections complications, Epstein-Barr Virus Infections drug therapy, Nasopharyngeal Neoplasms therapy
- Abstract
Background: Nasopharyngeal carcinoma (NPC) is a tumor caused by epithelial cells covering the surface of the nasopharynx. NPC only accounted for less than 1% of all cancers diagnosed worldwide. However, the global incidence rates are highest in southern China. We report a case of local advanced undifferentiated NPC [specifically, vesicular nucleus cell carcinoma (VNCC) of NPC]. Long-term disease-free survival (DFS) of a patient with stage IVA NPC is reported., Case Description: A 42-year-old male presented with a 4-month history of rhinorrhea and a lump in the left neck. The positron emission tomography (PET) showed local invasion to the surrounding tissues, specifically, the tumor invaded the brain. The pathological diagnosis was VNCC, the Epstein-Barr virus (EBV) was positive in tumor tissues by in situ hybridization. and the clinical diagnosis was stage IVA of NPC. The patient was treated with induction chemotherapy (IC) with gemcitabine and cisplatin (GP) followed by cisplatin/radiotherapy. The tumor lesions complete response (CR) after concurrent chemo-radiotherapy (CCRT)., Conclusions: To date, the DFS time has been more than 5 years. IC with GP followed by CCRT should be the first choice of treatment for patients with locoregionally advanced NPC. In recent years, more and more studies have shown the efficacy of immunotherapy in treating recurrent or metastatic NPC patients, especially in patients or are programmed death-ligand 1 (PD-L1)-positive or have a high tumor mutation burden. In the future, immunotherapy may become a standard treatment in clinic and bring longer survival to patients.
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- 2022
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21. Overexpression of ADAM9 decreases radiosensitivity of hepatocellular carcinoma cell by activating autophagy.
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Zhu L, Zhao Y, Yu L, He X, Wang Y, Jiang P, Yu R, Li W, Dong B, Wang X, and Dong Y
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- ADAM Proteins metabolism, Animals, Cell Line, Tumor, Humans, Male, Membrane Proteins metabolism, Mice, Mice, Nude, Up-Regulation genetics, ADAM Proteins genetics, Autophagy genetics, Carcinoma, Hepatocellular genetics, Carcinoma, Hepatocellular metabolism, Liver Neoplasms genetics, Liver Neoplasms metabolism, Membrane Proteins genetics, Radiation Tolerance genetics
- Abstract
A disintegrin and a metalloprotease (ADAM)9 upregulated within human hepatocellular carcinoma (HCC) cells, but its effect on HCC radiosensitivity remains unknown. The present work aimed to examine the effect of ADAM9 on HCC radiosensitivity and to reveal its possible mechanism, which may be helpful in identifying a potential therapeutic strategy. Changes in ADAM9 expression after X-ray irradiation were identified using western blot, qRT-PCR, and immunofluorescence. ADAM9 stable knockdown and overexpression cell lines were constructed using lentivirus packaging. The radiosensitivity of HCC cells with altered ADAM9 expression was examined by CCK-8 assays, subcutaneous tumorigenesis experiments, and clone formation assays. This study also determined how autophagy affected HCC cell radiosensitivity. Furthermore, ADAM9, p62 and Bax expressions in HCC tissues that were removed after radiotherapy were detected by immunohistochemistry, and the relationship among the levels of these molecules was statistically analyzed. The level of ADAM9expression in HCC cells increased after X-ray irradiation. Through CCK-8 assays, subcutaneous tumorigenesis experiments, and clone formation assays, this work discovered the increased MHCC97H cell radiosensitivity after ADAM9 knockdown, and the radiosensitivity of Huh7 cells decreased after the overexpression of ADAM9. Furthermore, ADAM9 induced HCC cell autophagy via downregulating Nrf2 expression, while autophagy inhibition or induction reversed the effects of altered ADAM9 expression on radiosensitivity. Moreover, ADAM9 level showed a negative correlation with Bax and p62 expression within HCC tissues after radiotherapy. Taken together, ADAM9 decreased the radiosensitivity of HCC cells, and autophagy mediated this process.
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- 2021
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22. Effect of fucoidan on ethanol-induced liver injury and steatosis in mice and the underlying mechanism.
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Xue M, Liang H, Zhou Z, Liu Y, He X, Zhang Z, Sun T, Yang J, Qin Y, and Qin K
- Abstract
Background: Alcoholic liver disease is caused as a result of chronic alcohol consumption. In this study, we used an alcoholic liver injury mouse model to investigate the effect of fucoidan on ethanol-induced liver injury and steatosis and the underlying mechanisms., Methods: All mice were randomly divided into four groups: 1) control group, 2) model group, 3) diammonium glycyrrhizinate treatment group (200 mg/kg body weight), and 4) fucoidan treatment group (300 mg/kg body weight). Administration of ethanol for 8 weeks induced liver injury and steatosis in mice., Results: Fucoidan treatment decreased serum alanine aminotransferase activity, serum total cholesterol levels, and hepatic triglyceride levels, and improved the morphology of hepatic cells. Fucoidan treatment upregulated the expression of AMPKα1, SIRT1, and PGC-1α and inhibited the expression of ChREBP and HNF-1α. The levels of hepatic IL-6 and IL-18 were significantly decreased in the fucoidan group. Further, the levels of cytochrome P450-2E1 (CYP2E1), glucose-regulated protein (GRP) 78, and 3-nitrotyrosine (3-NT) in hepatic tissues were reduced in the fucoidan group as compared to the model group. Fucoidan significantly reversed the reduction of ileac Farnesoid X receptor (FXR) and fibroblast growth factor 15 (FGF15) levels induced by alcohol-feeding and reduced CYP7A1 (cholesterol 7α-hydroxylase) expression and total bile acid levels in the liver tissue. In addition, fucoidan regulated the structure of gut flora, with increased abundance of Prevotella and decreased abundance of Paraprevotella and Romboutsia as detected by 16S rDNA high-throughput sequencing., Conclusion: Fucoidan inhibited alcohol-induced steatosis and disorders of bile acid metabolism in mice through the AMPKα1/SIRT1 pathway and the gut microbiota-bile acid-liver axis and protected against alcohol-induced liver injury in vivo., Competing Interests: The authors declare no conflicts of interest., (© 2021 Meilan Xue et al.)
- Published
- 2021
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23. Prognostic Significance of Serum Uric Acid and Gamma-Glutamyltransferase in Patients with Advanced Gastric Cancer.
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Yang S, He X, Liu Y, Ding X, Jiang H, Tan Y, and Lu H
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- Adult, Aged, Aged, 80 and over, Female, Gene Expression Regulation, Neoplastic, Humans, Kaplan-Meier Estimate, Male, Middle Aged, Multivariate Analysis, Nomograms, Prognosis, ROC Curve, Retrospective Studies, Biomarkers, Tumor blood, Stomach Neoplasms blood, Stomach Neoplasms mortality, Up-Regulation, Uric Acid blood, gamma-Glutamyltransferase blood
- Abstract
Purpose: In this study, we aim to evaluate the prognostic role of serum uric acid and gamma-glutamyltransferase in advanced gastric cancer patients., Methods: A total of 180 patients pathologically diagnosed with advanced gastric cancer were included in this retrospective study. We used time-dependent receiver operating characteristic (ROC) curves to identify the optimal cut-off value of serum uric acid (UA) and gamma-glutamyltransferase (GGT). Survival analysis was performed using the Kaplan-Meier method and log-rank test, and multivariate Cox regression analyses were applied. A nomogram was formulated, and the calibration and discrimination of the nomogram were determined by calibration curve and concordance index (C-index). We validated the results using bootstrap resampling and a separate study on 60 patients collected from 2015 to 2017 using the same criteria in other medical center., Results: Both higher serum uric acid (>228 μ mol/L) and higher gamma-glutamyltransferase (>14 U/L) had worse OS and PFS. Univariate analysis indicated that serum uric acid (UA) ( p < 0.001 and p < 0.001) and gamma-glutamyltransferase (GGT) ( p < 0.001 and p = 0.044) were significantly related to overall survival (OS) and progression-free survival (PFS), respectively. Multivariate analysis revealed serum uric acid (UA) and gamma-glutamyltransferase (GGT) were independent prognostic factors for OS ( p = 0.012, p = 0.001). The optimal agreement between actual observation and nomogram prediction was shown by calibration curves. The C-indexes of the nomogram for predicting OS and PFS were 0.748 (95% CI: 0.70-0.79) and 0.728 (95% CI: 0.6741-0.7819), respectively. The results were confirmed in the validation cohort., Conclusion: We observed that both serum UA and GGT were poor prognostic factors in patients with advanced gastric cancer. And we also formulated and validated a nomogram which can predict individual survival for advanced gastric cancer patients., Competing Interests: The authors have declared that no conflicts of interest exist., (Copyright © 2019 Shanshan Yang et al.)
- Published
- 2019
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24. Fucoidan Promotes Apoptosis and Inhibits EMT of Breast Cancer Cells.
- Author
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He X, Xue M, Jiang S, Li W, Yu J, and Xiang S
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- Animals, Cadherins genetics, Cadherins metabolism, Cell Movement drug effects, Female, Gene Expression Regulation, Neoplastic drug effects, Humans, MCF-7 Cells, Matrix Metalloproteinase 9 genetics, Matrix Metalloproteinase 9 metabolism, Random Allocation, Rats, Rats, Sprague-Dawley, Apoptosis drug effects, Breast Neoplasms drug therapy, Neoplasms, Experimental drug therapy, Polysaccharides pharmacology
- Abstract
Fucoidan is an active component of seaweed, and could inhibit proliferation and induce apoptotic cell death in several tumor cells. However, the function of fucoidan in breast cancer is largely unknown. In the present study, we evaluated the anti-cancer potential of fucoidan in human breast cancer MCF-7 cells. Adult Sprague-Dawley rats were randomized to receive fucoidan (200 or 400 mg/kg·body weight per day) or normal saline via gastric gavage for 3 consecutive days. Serum samples were prepared from these rats, and used for subsequent experiments to examine the potential effects in MCF-7 cells. Cell viability was determined using a 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Apoptosis was examined with Hoechst33258 staining and flow cytometry. Cell migration and invasion were measured by wound scratch assay and Transwell assay, respectively. Western blot and enzyme-linked immunosorbent assay (ELISA) were used to examine the expression of secretory E-cadherin and matrix metalloproteinase-9 (MMP-9). Conditioned serum from fucoidan-treated rats significantly suppressed cell proliferation and enhanced apoptosis. Cell migration and invasion were also significantly decreased. Observed effects of conditioned serum were associated with upregulation of E-cadherin and downregulation of MMP-9. Conditioned serum of rats treated with fucoidan could inhibit the proliferation and promote apoptosis of MCF-7 cells. Cell invasion and migration were inhibited, possibly via decreased epithelial-mesenchymal transition (EMT) process. Fucoidan may be a promising therapeutic agent for human breast cancers.
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- 2019
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25. A comparative study of 18F-FDG PET/CT and ultrasonography in the diagnosis of breast cancer and axillary lymph node metastasis.
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He X, Sun L, Huo Y, Shao M, and Ma C
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- Adult, Aged, Axilla, Breast Neoplasms diagnostic imaging, Breast Neoplasms pathology, Female, Humans, Lymph Nodes pathology, Middle Aged, Multimodal Imaging methods, Retrospective Studies, Sensitivity and Specificity, Young Adult, Breast Neoplasms diagnosis, Fluorodeoxyglucose F18 chemistry, Lymph Nodes diagnostic imaging, Lymphatic Metastasis diagnostic imaging, Positron Emission Tomography Computed Tomography methods, Radiopharmaceuticals chemistry, Ultrasonography methods
- Abstract
Background: The aim of this study was to compare the diagnostic value of 18F-FDG-PET/CT (PET/CT) with ultrasonography (US) in detection of primary breast cancer and axillary lymph nodes (ALN) metastasis of breast cancer., Methods: One hundred and sixty four patients with breast carcinoma were recruited and analyzed retrospectively. All patients underwent PET-CT and US. The PET/CT scan results for the diagnosis of primary breast cancer were compared with US. The diagnostic accuracy of PET/CT in detecting ALN metastasis was compared with histopathology., Results: In 164 patients with cytologically established breast carcinoma, the sensitivity of PET/CT and US in the diagnosis of breast cancer were 86% (141/164), 91% (149/164), respectively. The sensitivity, specificity of PET/CT and US in ALN staging were 46% and 54%, 91% and 91%, respectively. The diagnostic accuracy of PET/CT correlated with the ALN size, the SUVmax of primary breast cancer (P=0.02 and 0.04)., Conclusions: PET/CT is very expensive, and not superior to US in detection of primary breast cancer and in ALN staging, but superior in detecting distant metastases. PET/CT cannot be recommended as a primary diagnostic procedure in early breast cancer. US should still remain the first line for the diagnosis of stage I breast cancer. In relation to the detection of axillary node metastases, both PET/CT and ultrasonography have poor sensitivity, and cannot replace staging by using the sentinel node procedure.
- Published
- 2017
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26. Apoptosis is induced by docosahexaenoic acid in breast cancer cells via death receptor and mitochondria-mediated pathways.
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Xue M, Ge Y, Yu C, Zheng Z, He X, and Zhao J
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- Breast Neoplasms genetics, Breast Neoplasms metabolism, Caspases metabolism, Cell Line, Tumor, Cytochromes c metabolism, Female, Gene Expression, Humans, Proto-Oncogene Proteins c-bcl-2 genetics, Proto-Oncogene Proteins c-bcl-2 metabolism, Receptors, Death Domain genetics, Apoptosis drug effects, Docosahexaenoic Acids pharmacology, Mitochondria drug effects, Mitochondria metabolism, Receptors, Death Domain metabolism, Signal Transduction drug effects
- Abstract
In the present study, the antitumor effect of n‑3 fatty acid was evaluated, and the effect of docosahexaenoic acid (DHA) on the induction of apoptosis and its underlying mechanism were examined. Flow cytometry and western blot analysis were performed to analyze apoptosis and the expression of protein factors in human breast cancer cells. The data revealed that DHA inhibited the viability of MCF‑7 breast cancer cells in vitro, and promoted cell death by the induction of apoptosis. DHA decreased the expression of B‑cell lymphoma 2 (Bcl‑2), whereas the expression of Bcl‑2‑associated X protein was increased. DHA was also shown to promote the release of Smac/Diablo and cytochrome c from the mitochondria. DHA increased the levels of cleaved caspase‑8, ‑9 and ‑3. Additionally, the protein expression of tumor necrosis factor‑related apoptosis‑inducing ligand, death receptor 4 and Fas were increased following DHA treatment. In conclusion, DHA caused apoptosis of the human breast cancer cells in vitro through the death receptor and mitochondria‑mediated pathways. The results of this study encourage further investigation of the effect of fish oil on the prevention and treatment of human breast cancer.
- Published
- 2017
- Full Text
- View/download PDF
27. Therapeutic effects of cytoprotective agent on breast reconstruction after breast cancer surgery.
- Author
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He X, Wang L, Li W, Yu Z, and Wang X
- Abstract
Most patients will choose breast reconstruction after breast cancer surgery, while radiotherapy will damage skin and soft tissue so that will have adverse effect on reconstruction. In this study, we assume that the usage of Amifostine can reduce the incidence of complications after breast reconstruction so that provides more choices of reconstruction operation. Dividing SD rats into surgical placement expansion material group (include 15 ml normal saline) and simple operation group. Then further divide the former into non intervention group , radiation group and Radiation therapy combined with Amie amifostine treatment group. The decubation is 45 days after operation. Macroscopic evaluate the complications of skin and soft tissue by ImageJ. There is no obvious complications of skin and soft tissue for control group, radiotherapy alone group and radiotherapy with application of Amifostine group by macroscopic evaluation. The animals that are in expanded object group, damage probability of skin and soft tissue when use Amifostine is lower than that of radiotherapy alone group (30% vs. 69%, P=0.041). ImageJ shows the necrosis probability of skin and soft tissue when use Amifostine is obvious lower than radiotherapy alone group (6.96% vs. 12.94%, P=0.019). In conclusion, prevention and treatment of Amifostine can significantly reduce the complications of skin and soft tissue which is helpful to breast reconstruction after breast cancer surgery.
- Published
- 2015
28. Is it practical and cost effective to detect differentiated thyroid carcinoma metastases by (18)F-FDG PET/CT, by (18)F-FDG SPET/CT or by (131)I SPET/CT?
- Author
-
He X, Wang X, Yu J, and Ma C
- Subjects
- Adult, Bone Neoplasms diagnostic imaging, Humans, Lung Neoplasms radiotherapy, Lung Neoplasms secondary, Male, Neoplasm Metastasis, Prognosis, Whole Body Imaging, Fluorodeoxyglucose F18, Iodine Radioisotopes, Multimodal Imaging, Positron-Emission Tomography, Thyroid Neoplasms diagnostic imaging, Tomography, Emission-Computed, Single-Photon, Tomography, X-Ray Computed
- Abstract
Fluorine-18-labeled 2-fluoro-2-deoxy-D-glucose positron emission tomography/computerized tomography ((18)F-FDG PET/CT) plays an important role in detecting differentiated thyroid carcinoma (DTC) metastases with elevated thyroglobulin (Tg) and negative radioiodine ((131)I) uptake. In conclusion, (18)F-FDG PET/CT may fail to detect all DTC metastases, while (131)I WBS combined with (131)I SPET/CT may be a better cheaper and diagnostic tool as suggested by the case we presented here. Positive metastases in both (131)I and (18)F-FDG SPET/CT may indicate worse prognosis. Future research may add more evidence as to which is the best diagnostic imaging modality and relate it to the molecular mechanism of the uptake of the radionuclide used.
- Published
- 2015
- Full Text
- View/download PDF
29. The potential role of Ku80 in primary central nervous system lymphoma as a prognostic factor.
- Author
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Li X, He X, Xu X, Song Z, Qian C, Wang J, and Wang Y
- Abstract
The aim of our study was to detect the expression of Ku80 in primary central nervous system lymphoma and to evaluate the relationship between Ku80 expression level and clinical outcomes. Thirty-eight patients with primary central nervous system lymphoma (PCNSL) were included in this retrospective study. The expression of Ku80 in tumor samples was determined by immunohistochemistry. One thousand neoplastic cells per specimen were counted. The expression levels were compared with the clinical data and statistically analyzed. The results of this study show that the expression of Ku80 can be found in the majority of PCNSLs. The mean expression level of Ku80 in 38 PCNSL is 64.1 ±24.5. A significant difference in Ku80 expression could be found between the age < 65 years group and age ≥ 65 years group (P = 0.006). Kaplan-Meier analysis revealed that patients who showed a high Ku80 expression had a significantly shorter median survival time (MST) than patients who had low Ku80 expression (P = 0.036). Patients' age, tumor location, and treatment protocol were significantly related to prognosis in PCNSL (P < 0.05). The expression of Ku80 was observed in the majority of PCNSLs. Ku80 was a predictive factor for survival in this study. In addition to Ku80, other clinical variables including age, tumor location and therapeutic protocol are correlated significantly with overall survival.
- Published
- 2013
- Full Text
- View/download PDF
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