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Using “Lianjiu No.1”, “Yuntaihongjiu” and “Yuntaijinjiu” as research objects, the anthocyanin components and content of their leaves before and after color transformation were qualitatively and quantitatively analyzed using ultra-high performance liquid chromatographymass spectrometry technology.The results showed that a total of 14 anthocyanins were detected in leaves from three varieties, including malvidin, pelargonidin, delphinidin, peonidin, cyanidin 3-galactoside, isorhamnetin, cyanidin, cyanidin 3, 5-diglucoside, petunidin, quercetin, rutin, procyanidin B4, procyanidin B2 and kaempferol. Cyanidin 3-galactoside and rutin were the main anthocyanin components of three varieties. The cyanidin 3-galactoside was the main pigment component for the red colored leaves of “Yuntaihongjiu”; rutin was the main reason for the yellow colored leaves of “Yuntaijinjiu”. The main anthocyanins types in three varieties were rutins, cyanidins and others. After the color change of leaves, the content of cyanidins from high to low was in order of “Lianjiu No.1” > ”Yuntaihongjiu” > ”Yuntaijinjiu”. After the color change of leaves, the content of rutins from hight to low was in order of “Yuntaijinjiu” > “Lianjiu No.1” > “Yuntaihongjiu”. [ABSTRACT FROM AUTHOR]

Objective: This study aims to investigate the molecular mechanisms of Huangqi {Astragalus) in the treatment of premature ovarian insufficiency (POI) using network pharmacology and molecular docking methods. Methods: The active compounds and target proteins of Huangqi were screened from the TCMSP database, while the disease target proteins associated with POI were obtained from the Genecards database. The Venny online tool was used to identify the overlapping genes between the two datasets. Cytoscape software was used to construct a gene regulatory network for the active ingredients of Huangqi. The protein-protein interaction (PPI) network and core genes were selected based on the STRING database and CytoNCA plug-in of Cytoscape. GO (gene ontology) enrichment and KEGG (Kyoto encyclopedia of genes and genomes) pathway enrichment analyses of the overlapping genes were performed using the DAVID database. Molecular docking techniques were used to further elucidate the molecular mechanisms underlying the therapeutic effects. Human ovarian granulosa-like tumor cells (KGN) underwent serum starvation (SS) treatment to induce POI cell models and then received quercetin treatment, and experimentally validated the network pharmacology prediction results by TUNEL staining and Western blotting assay. Results: The TCMSP database provided 32 active compounds and 552 target genes associated with Huangqi, while totally 730 disease target genes associated with POI were screened out from the disease database. Of these, 62 genes were found to be shared between the two datasets. The top 6 compounds based on degree values were quercetin, isorhamnetin, kaempferol, mairin, formononetin and astragaloside IV. The 10 core genes identified were ALB, INS, AKT1, ACTB, TP53, TNF, ESR1, CASP3, STAT3 and PTEN. These potential targets were mainly involved in regulating the JAK2/STAT3 and PI3K/Akt signaling pathway. Molecular docking results revealed strong binding interactions between the compounds and the key proteins STAT3 and Akt1 in the JAK-STAT and PI3K-Akt signalling pathways. The results of in vitro experiments revealed that quercetin could activate the JAK2/STAT3 pathway and inhibit cell apoptosis. Conclusions: Network pharmacology and molecular docking analysis showed that Astragalus could regulate the response of ovarian cells to estradiol, inhibit apoptosis and regulate the JAK-STAT and PI3K-Akt signalling pathways in the treatment of early onset ovarian dysfunction. [ABSTRACT FROM AUTHOR]

Objective: To investigate whether galangin (Gal) affects the proliferation, migration, invasion and apoptosis of osteosarcoma MG63 cells by regulating the cGAS/STING signaling pathway. Methods: Human osteosarcoma MG63 cells were cultured in vitro and treated with Gal at concentrations of 0, 5, 15, 25, 50, 100 and 200 μmol/L for 48 hours, and the effect of Gal on cell viability was detected by CCK-8 method. MG63 cells were divided into the control group (untreated cells), the Gal low concentration group (Gal-L group, treated with 50 μmol/L Gal), the Gal high concentration group (Gal-H group, treated with 100 μmol/L Gal), and the Gal-H+STING inhibitor group (Gal-H+H-151 group, treated with 100 μmol/L Gal+8 μmol/L H-151). EdU staining method, scratch healing test, Transwell chamber method and flow cytometry were used to detect cell proliferation, migration, invasion and apoptosis of cells in each group. Western blot was applied to detect the expression levels of cGAS and STING proteins in cells of each group. Results: Compared with the control group, the proliferation activity, migration, and invasion abilities of the cells in the Gal-L and Gal-H groups were significantly decreased (all P<0.05); the apoptosis rate and the expression levels of cGAS and STING proteins were significantly increased (all P<0.05). Compared with the Gal-H group, the proliferation activity, migration, and invasion abilities of the cells in the Gal-H+H-151 group were significantly increased (all P<0.05); the apoptosis rate and the expression levels of cGAS and STING proteins were significantly decreased (all P<0.05). Conclusion: Gal may inhibit the proliferation, migration and invasion and promote the apoptosis of osteosarcoma cells by activating the cGAS/STING signaling pathway. [ABSTRACT FROM AUTHOR]

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This study aimed to comparatively analyze the flavonoid contents of the purple-colored cultivar Xianghongtai No. 1 (XHT1H, R) and the green-colored cultivar Yinong 50 tian Caixin (YN50T, G). The flavonoid compounds were separated and identified by ultra-performance liquid chromatography-tandem mass spectrometry. Principal component analysis (PCA), hierarchical cluster analysis, and orthogonal partial least partial-least squares discrimination analysis (OPLS-DA) were used to analyze metabolomics data and screen for differential metabolites in Hong Caitai and Caixin. PCA revealed significant differences in the flavonoid compound contents between the two varieties. OPLS-DA identified 170 different flavonoid metabolites, including 56 flavonols, 38 anthocyanidins, and 32 flavones. The anthocyanins included 25 cyanidin glycosides, six delphinidin glycosides, three peonidin glycosides, and three petunidin glycosides. Additionally, six of the top ten metabolites with higher content in Hong Caitai than in Caixin were cyanidins. Based on the differential flavonoids profiles of the two varieties, cyanidin, delphinidin, peonidin, and petunidin were associated with the pigmentation in Hong Caitai. These results provide a reference for further study of the mechanism of stem color formation and pigment utilization in Caitai. [ABSTRACT FROM AUTHOR]

Under the background of toxic and harmful petroleum based adhesives and the rapid depletion of fossil resources, bio-based adhesives have developed rapidly. As an important class of plant tannins, condensed tannins (CTs) possess flavonol units and represent up to 90% of global commercial tannins resources. CTs present unique chemical properties owing to their special polyphenol structures, which is more suitable to replace phenol in adhesives either in reactivity or economy, for its reaction rate with formaldehyde is 3 times that of phenol. In this way, the research progress of bio-based adhesives in recent years was highlighted and a prospect was made in this review, in order to provide reference for the development and application of condensed tannins adhesives. [ABSTRACT FROM AUTHOR]

Pimpinella candolleana is known as Miao ethnic herbal medicine in Guizhou for the treatment of icteric hepatitis, acute cholecystitis and other diseases. To investigate the chemical constituents of P. candolleana and their anti-inflammatory activities, the chemical constituents from the 70% ethanol extract of P. candolleana were separated by silica gel, Sephadex LH-20, Toyopearl HW-40F, Toyopearl HW-40C, ODS and other column chromatography technologies, and their structures were elucidated by extensive spectroscopic analysis such as nuclear magnetic resonance(NMR)and mass spectrum(MS). The inflammatory cell model, built by LPS-induced RAW264.7 macrophage cells, was used to evaluate the anti-inflammatory activity. The results were as follows:(1)Twenty compounds from P. candolleana were isolated and identified of including vanillin(1), sesamin(2), 2-methyl-2-hydroxy-5-methoxy berz(d)hydrofuran-3-one(3), procatechin(4), 1,5-dihydroxy-2,3-dimethoxyxanthone(5), isorhamnetin(6), kaempferol(7), 8-hydroxy-2-methylchromone(8), luteolin(9), quercetin(10), 1-O-β-D-glucopyranosyl-(2S,3S,4R,8E)-2-[(2'R)-2'-hydroxypalmitoylamino]-8-octadecene-1,3,4-triol(11), isorhamnetin-3-O-β-D-galactopyranoside(12), isoquercitrin(13), norswertianolin(14), luteolin-6-C-α-L-arabinoside(15), kaempferol-3-O-β-D-galactopyranoside(16), kaempferol-7-O-β-D-glucopyranoside(17), luteolin-7-O-β-D-glucopyranoside(18), isovitexin(19), rutin(20). Compounds 1, 3, 4, 6, 7, 10, 13, 16, 18, and 20 were obtained from this plant for the first time.(2)The anti-inflammatory results showed that compounds 2-10, 12, 18 and 19 could significantly inhibit the LPS-induced NO content in RAW264.7 cells(P<0.05, P<0.01), and the inhibition rates of compounds 4, 7, 10, and 18 at a concentration of 25 μmol·L-1 were 57.37%, 83.60%, 68.16%, 81.14%, respectively. Overall, this study enriches the chemical constituents of P. candolleana, and clarifies that flavonoids are the active ingredients in the course of anti-inflammatory, which provides a theoretical reference for further research and exploitation of P. candolleana. [ABSTRACT FROM AUTHOR]

Copyright of Acta Agriculturae Zhejiangensis is the property of Acta Agriculturae Zhejiangensis Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Sea bucthorn (Hippophae rhamnoides) fruits are gradually used to develop various functional foods, due to their high edible and medicinal values rich in nearly 200 kinds of nutrients and active substances, such as polyphenols, polysaccharides, carotenoids, and flavonoids. This present research aims to clarify the difference of Sea bucthorn from the representative places of origin, in order to provide the selection basis for the research and development of the Sea bucthron nutritious products. The Sea bucthorn was collected from Shanxi, Hebei, Inner Mongolia, and Xinjiang in China. The varieties of Sea buckthorn were mostly used in the processing (Hippophae rhamnoides. L. Zhongguoshaji, Hippophae rhamnoides. L. Yulushaji, Hippophae rhamnoides. L. Neimenggushaji, and Hippophae rhamnoides. L. Altaydaguoshaji). The quality of fruit was comprehensively evaluated from the aspects of color, texture, taste, flavor, and nutritional components. Gas Chromatography-Mass Spectrometry (GC-MS) and Ultra-High Performance Liquid Time-Of-Flight Mass Spectrometry (UHPLC-QTOF-MS) were selected to determine the volatile components and small molecule active substances. The comprehensive quality of Sea buckthorn was explored to determine the difference in four regions of China. The results showed that the Sea bucthorn from Xinjiang was the brightest among the four regions. The highest sugar-acid ratio (1.997) was found in the Sea bucthorn from Inner Mongolia. The sea bucthorn from Hebei Province presented the highest polyphenol content (1.14 mg/g). By contrast, Xinjiang Sea buckthorn shared the highest carotenoid content (37.15 mg/100 g). However, there was no significant difference (P>0.05) in the vitamin C content of the sea buckthorn from four regions (21-23 mg/100 g). Moreover, there was a significant difference (P<0.05) in the antioxidant activity of sea bucthorn from the four regions. In addition, the Shanxi Sea bucthorn possessed the highest scavenging rate of DPPH free radical. Inner Mongolia sea buckthorn presented the highest ABTS free radical scavenging rate and iron ion reduction ability (FRAP).A total of 153 compounds were detected in the sea buckthorn from the four regions, indicating the significant difference in the volatile components. The characteristic volatile substances in the Hebei sea buckthorn were 3-octanone, 2-heptenal, and amyl isobutyrate, whereas, there were the isopropyl isovalerate, propyl 2-methylbutyrate, and (methylthiol) ethyl acetate in Inner Mongolia. The n-octane,2, 3-dihydro2,2, 6-trimethylbenzalde hyde was found in the XinJiang sea buckthorn, whereas, the amyl hexanoate, basil, and ethyl decanoate were in the Shanxi one. 61 compounds were detected in the small molecular active components, among which the main polyphenols were quinic acid, isorhamnetin, aloxone sugar, kaempferol, and quercetin. On the whole, the Inner Mongolia sea buckthorn was the strongest antioxidant capacity and the highest sugar-acid ratio, indicating a promising potential raw material for functional products and health products. The characteristic components of sea buckthorn from the Shanxi were 1, 3-dihydroxy-12, 24-dienoic acid, quinic acid, and 2- [(2-ethyl butyryl) amino]-4, 5-dimethoxybenzoic acid. The characteristic components of sea buckthorn from Xinjiang were mainly 1-oleyl-glycerol-3-phosphocholine, and aloxone. A comprehensive comparison was performed on the physicochemical characteristic of representative sea bucthoron in the XinJiang, Inner Mongolia, Shanxi, and Heibei regions. A factor analysis was carried out on the 14 quality indexes of Sea buckthorn. Among them, three common factors were extracted, where the cumulative variance contribution rate reached 100%. The contribution rate of the first common factor reached 45.831%, followed by 38.623%, and 15.546%. The comprehensive quality scores of the representative sea buckthorn varieties were ranked in descending order of the Hebei, Shanxi, Inner Mongolia, and Xinjiang sea buckthorn, according to the comprehensive quality evaluation model. A better performance was achieved to compare the color, taste, flavor, active component, and antioxidant quality of sea buckthorn in four regions of China. The finding can provide the technical reference and application approaches for the raw material selection in the series of sea buckthorn products. [ABSTRACT FROM AUTHOR]

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Copyright of Science & Technology of Cereals, Oils & Foods is the property of Science & Technology of Cereals, Oils & Foods Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Brewing is the property of China Brewing Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The root of Kadsura coccinea is a commonly used herb in traditional Chinese medicine, and flavonoids are one of the main active constituents of medicinal plants. This study intended to investigate whether the leaves and stems of K. coccinea plants may contain similar or highly enriched flavonoids in roots and to explore its application value. Widely-targeted metabolomics was used to identify the compounds in different organs of K. coccinea. According to their structural distribution and categories, the diversity and abundance of flavonoids were analyzed. The results were as follows:(1)The number of flavonoids metabolites in K. coccinea was sorted in descending order as leaf(80)> stem(73)> root(67), and a total of 61 flavonoids were the same in three parts. Leaves and stems contained more flavonols, contributing to a diversity of flavonoids greater than that in the roots.(2)Flavonols, flavonoids, flavanols, and chalcones were highly accumulated in the three parts. Among them, the accumulation of flavonols and flavonoids was lower in the stems and roots, resulting in a continuously, substantially decreasing abundance of flavonoids from leaf(24.00 × 107)to stem(13.45 × 107)to root(9.05 × 107).(3)Leaves and stems of K. coccinea contained a large amount of flavonoids that were also found in the roots and a variety of those not found in the roots, which could be considered for alternative or complementary use. Catechins highly expressed in all three parts, and the leaves were also rich in quercetin and its derivatives, which were highly valuable in application. [ABSTRACT FROM AUTHOR]

Copyright of Modern Food Science & Technology is the property of Editorial Office of Modern Food Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of Journal of Food Safety & Quality is the property of Journal of Food Safety & Quality Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To analyze the pharmacological mechanism of Huang’e capsule in the treatment of benign prostatic hyperplasia based on network pharmacology method and molecular docking. Methods The effective components and related targets of 12 herbs in Huang’e capsules were obtained by TCMSP（Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform）. Genecards, OMIM and TTD databases were used to identify the targets of benign prostatic hyperplasia. The common target genes of drugs and diseases were obtained by using R software. The STRING platform was applied to analyze protein interactions and construct PPI networks. Metascape platform was applied for GO and KEGG enrichment analysis of common genes, and the data were imported into Cytoscape3.8.2 to construct the network of " drug ingredients-targets-passways".PyMOL,AutoDockTools 1.5.6 and AutoDock Vina software were used for molecular docking. Results Quercetin, kaempferol and β-sitosterol were the core active components of Huang’e capsule in the treatment of BPH. The ideal targets were p53, MAPK1, ADRα1a, AKT1, ED-1, etc. Molecular docking verification showedthat the binding energy <-5.0 kcal·mol-1accounted for 93.3% of the total, proving that the core active components had excellent binding activity with core targets. The signal pathways of Huang’e capsule targeting benign prostatic hyperplasia mainly focused on PI3K-Akt signaling pathway, IL-17 signaling pathway,FoxO signaling pathway,whose functions mainly include apoptosis signaling pathway, oxidative stress and other processes. Conclusion This study proposes the possible mechanism by which Huang’e capsule controls BPH by inhibiting the proliferation of prostatic cells and relieving the pressure of posterior urethra. [ABSTRACT FROM AUTHOR]

Objective To investigate the mechanism of Ningmitai Capsule in the treatment of chronic prostatitis（CP）and benign prostatic hyperplasia（BPH） using network pharmacology. Methods The ingredients and action targets of Ningmitai Capsule were retrieved through TCMSP and BATMAN-TCM database, the action targets of CP and BPH were retrieved through gene cards, OMIM and Disgenet database, the common targets of CP and BPH were retrieved through Venn diagram tool and the effective targets of Ningmitai Capsule in the treatment of CP and BPH were retrieved, and the consensus gene to construct a protein interaction（PPI） network by string database, The core targets were screened using Cytoscape software to construct a "drug-component-target" network. Go and KEGG enrichment analyses were performed using the metascape database. Molecular docking validation of core targets and constituents was performed using autodock4.2.6. Results 47 active ingredients of Ningmitai Capsule with 280 targets and 1445 disease targets were obtained after screening,while 168 potential therapeutic targets were obtained after intersection, the core ingredients including quercetin, kaempferolβ-Sitosterol, among others, and core targets include AR, ESR1, TNF, IL-1B, among others. Go analysis showed that the therapeutic targets were associated with processes such as response to inorganic substances, apoptotic signaling pathway,response to lipopolysaccharide, mainly involving PI3K/Akt, MAPK and other signaling pathways. Conclusion Ningmitai Capsule may achieve the effects of "Treat Different Diseases with the Same Method" for CP and BPH by inhibiting the synthesis of proinflammatory cytokines, inhibiting signal transduction, regulating cell growth in prostate tissue, regulating androgen levels, and other mechanisms. [ABSTRACT FROM AUTHOR]

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Copyright of Environmental Science & Technology (10036504) is the property of Editorial Board of Environmental Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Modern Food Science & Technology is the property of Editorial Office of Modern Food Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Food Safety & Quality is the property of Journal of Food Safety & Quality Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Fagopyrum plant is rich in resources and contained a large number of flavonoid compounds. Research history and the research hotspots of buckwheat flavonoids were summarized by literature retrieval in this study. As it was revealed, the research paper about buckwheat flavonoids was firstly published in 1952. From 1952 to 1999, the number of publishing papers was less than 10 papers per year, and the research on buckwheat flavonoids was in its infancy. Since the year of 2000, buckwheat flavonoids have gradually gained more attentions from researchers, and the numbers of publishing papers have been increased annually. In the past two decades, researches conducting buckwheat flavonoids studies mainly focused on the fields of botany, food science and technology, agriculture, and biochemistry and molecular biology. Articles referring to the antioxidant activity of buckwheat flavonoids got high citations, and the biological activities and nutritional functions of buckwheat flavonoids attracted much attention. By now, 80 flavonoid compounds have been identified from Fagopyrum. Of them, quercetin, kaempferol, luteolin, rhamnetin, isorhamnetin, tricin, naringenin, myricetin, apigenin and hesperetin were the common feature flavonoid aglycones from buckwheat. Peonidin, delphinidin and cyanidin were the anthocyanins compounds which were mostly isolated from Fagopyrum. Biosynthesis of buckwheat flavonoids originated from the phenylpropane metabolism pathway, and the key enzymes genes involved in the pathway, such as PAL, CHS, C4H, 4CL, CHI, LAR etc., and the transcription factor MYB genes were cloned and identified. MYB transcription factors played an important role in the induction and regulation of flavonoid biosynthesis, and major influence factors for the flavonoids biosynthesis included the environment factors, plant growth regulators, biological factors and varieties, and these factors could have cross regulations effects on the buckwheat flavonoids biosynthesis. In this paper, research overviews of buckwheat flavonoids, flavonoid compounds, flavonoids biosynthesis pathway, mechanism of regulation, and major influence factors are summarized, which can lay a theoretical foundation and a feasible scheme for high quality of buckwheat production during the cultivation process, and promotion of nutritional values of buckwheat products, and point out the research direction of buckwheat flavonoids in the future. [ABSTRACT FROM AUTHOR]

Objective: To study the active components, targets and biological significance of Redlining and Xuebijing by using network pharmacology, and also to explore the mechanism of treating Corona Virus Disease 2019 (COVID-19) through "treating the same disease with different methods". Methods: The components and targets of Redlining and Xuebijing were obtained via the access of Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). COVID-19-related proteins were screened in GeneC ards. Cytoscape software, ST RING, ClueGo tool and molecular docking were used to respectively construct the active component target network and protein interaction network, and also to make pathway enrichment analysis and verification, Results: The results of network analysis showed that the common components of the two compounds included luteolin, quercetin, kaempferol, isorhamnetin and phytosterol, of which quercetin, luteolin and kaempferol displayed certain binding ability to 3CLpro. There were 65 unique components in Xuebijing and 24 unique components in Redlining. These components might play an anti-CO VID-19 role through vascular endothelial growth factor-A (VEGF-A), CASP3, IL--6, M APK1, MA PIC 8 and other key proteins, as well as Jak-STAT, PI3K--Akt, MAPK and TGF--P signaling pathways. Conclusion: Due to component variations, Redlining and Xuebijing have both the same and different anti-CO VID-19 targets. Through the analysis of targets and pathways, a preliminarily explanation was presented to clarify the scientific connotation of "treating the same disease with different methods" in the treatment of COVID-19. [ABSTRACT FROM AUTHOR]

A high performance liquid chromatography (HPLC) method was established by optimizing the mobile phase to determine the mobile phase content of flavonol glycosides in Ginkgo biloba extract. Under the mobile phase conditions of the chromatographic column EC-C18 (4 m, 4.6 mm x 150 mm), the column temperature is 25 °C, the flow rate is 1.0 mL/min, the detection wavelength is 370 nm, the methanol, water and acetonitrile (60 • 35 ! 5 by volume),the content of quercetin, kaempferol and isorhamnetin in the flavonol glycosides were determined. The results show that under the determined optimal conditions, the separation effect of the three substances is great, and there is a good linear relationship in the range of detection concentration. Besides, the average spiked recovery rates of this three compounds are 99.26%, 100.1% and 100.35%. The method is simple and rapid, and can be used for the accurate determination of the contents of three substances in flavonol glycosides. [ABSTRACT FROM AUTHOR]

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Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Forest Research is the property of Forest Research Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

【Objective】The objective of the study was to develop a method to extract and detect rutin, kaempferol-3-O-rutinoside, quercetin, and kaempferol from cassava leaves. 【Method】The extraction was conducted at 70 °C with 40%(rutin, kaempferol-3-O-rutinoside) and 80%(quercetin and kaempferol) ethanol aqueous solution. The liquid/solid ratio was 1/75(g/mL) and ultrasonic extraction time was30 min. The mobile phase was eluted with methanol 0.2% phosphoric acid aqueous solution. The detection wavelength was 360 nm, the flow rate was 0.8 mL/min and the column temperature was 40 °C. 【Result】The results showed that the method could effectively extract and isolate rutin, fireworks glycosides, and quercetin. The linear relationship of the four flavanols(rutin, kaempferol-3-O-rutinoside, quercetin and kaempferol) was good(R2 0.9992), and the detection limit were 0.02-0.10 μg/mL. The accuracy of the method is good: the standard deviation(RSD) of precision, repeatability and stability of the method is less than 4.00%; the average recovery of the sample is90.81%-99.83%, and RSD 2.84%. By applying this method, the contents of flavonols in different growth stages and different varieties of cassava leaves were determined. The flavonols contents order were unfolded young leaves young leaves mature leaves. 【Conclusion】The extraction and detection method is simple, accurate and reproducible, and can simultaneously determine four flavonols in different growth stages and different varieties of cassava leaves. [ABSTRACT FROM AUTHOR]

The content of total flavonol glycosides in Ginkgo Folium in the planting bases was determined by high performance liquid chromatography(HPLC).The samples were extracted by reflux with methanol-25% hydrochloric acid.The HPLC conditions were as follows: Agilent ZORBAX SB-C&#95;(18) column(4.6 mm×250 mm, 5 μm), isocratic elution with mobile phase of 0.4% phosphoric acid solution-methanol(45∶55), flow rate of 1 mL·min~(-1), column temperature of 30 ℃, detection wavelength of 360 nm, and injection vo-lume of 10 μL.A method for the determination of terpene lactones in Ginkgo Folium was established based on ultra-high performance liquid chromatograph-triple-quadrupole/linear ion-trap tandem mass spectrometry(UPLC-QTRAP-MS/MS).The UPLC conditions were as below: gradient elution with acetonitrile-0.1% formic acid, flow rate of 0.2 mL·min~(-1), column temperature of 30 ℃, sample chamber temperature of 10 ℃, and injection volume of 10 μL.The ESI~+and multiple reaction monitoring(MRM) were adopted for the MS.The above methods were used to determine the content of total flavonol glycosides and terpene lactones in 99 batches of Ginkgo Folium from 6 planting bases, and the results were statistically analyzed.The content of flavonoids and terpene lactones in Ginkgo Folium from different origins, from trees of different ages, harvested at different time, from trees of different genders, and processed with different methods was compared.The results showed that the content of total flavonol glucosides in 99 Ginkgo Folium samples ranged from 0.38% to 2.08%, and the total content of the four terpene lactones was in the range of 0.03%-0.87%.The method established in this study is simple and reliable, which can be used for the quantitative analysis of Ginkgo Folium.The research results lay a basis for the quality control of Ginkgo Folium.

Objective: To investigate the effect of dihydromyricetin (DHM) on cardiac insufficiency in diabetic rats and explore the underlying mechanism., Method: Twenty-four male SD rats were randomized equally into normal control group, type 2 diabetes (T2DM) group fed on a high-glucose and high-fat diet for 6 weeks with low-dose streptozotocin (STZ) injection, metformin (MET) group with daily intragastric administration of MET (150 mg/kg) for 8 weeks after T2DM modeling, and dihydromyricetin (DHM) group with daily intragastric administration of DHM (250 mg/kg) for 8 weeks after modeling. The levels of fasting blood glucose, low density lipoprotein (LDL-C), triglyceride (TG), total cholesterol (TC), high density lipoprotein (HDL-C) and glycosylated hemoglobin (HbA1c) of the rats were measured, and plasma levels of insulin and high mobility group protein-1 (HMGB1) were detected with ELISA. The cardiac function of the rats was assessed using color echocardiography, ECG was measured using a biological signal acquisition system, and myocardial pathology was observed with HE staining. The protein expressions of HMGB1, nuclear factor-κB (NF-κB) p65 and phospho-NF-κB p65 (p-NF-κB p65) in the myocardial tissue were detected using Western blotting., Results: Compared with the control group, the rats in T2DM group showed significant anomalies in cardiac function after modeling with significantly increased plasma HMGB1 level and expressions of HMGB1, NF-κB p65 and p-NF-κB p65 proteins in the myocardial tissue ( P < 0.05 or 0.01). Treatment with DHM significantly improved the indexes of cardiac function of the diabetic rats ( P < 0.05 or 0.01), decreased plasma HMGB1 level and down-regulated the protein expressions of HMGB1 and p-NF-κB p65 in the myocardial tissue ( P < 0.05 or 0.01)., Conclusion: DHM treatment can improve cardiac function in diabetic rats possibly by down-regulation of HMGB1 and phospho-NF-κB p65 expressions in the myocardium.

Objective: To study the effects of dihydromyricetin (DMY) on the proliferation, apoptosis and epithelial mesenchymal transition (EMT) of esophageal squamous cell carcinoma (ESCC) cell KYSE150 and KYSE410. Methods: KYSE150 and KYSE410 cells were treated with different concentrations of DMY (0, 25, 50, 100, 150, 200 μmol/L) for 24 hours. The median inhibition concentration (IC 50 ) values of KYSE150 and KYSE410 were detected by cell counting kit-8 (CCK-8) method. Then 0.5‰ dimethyl sulfoxide (DMSO) was used as control group, dihydromyricetin (DMY), dihydromyricetin and transforming growth factor-β1 (DMY+ TGF-β1), transforming growth factor-β1 (TGF-β1) were used as experimental group. Cell proliferation and apoptosis rates were measured by clonal formation and flow cytometry. Transwell invasion and wound healing assay were used to detect cell invasion and migration. The protein expression levels of Caspase-3, Caspase-9, Bcl-2, Bax, Smad2/3, phosphorylation-Smad2/3 (p-Smad2/3) and Vimentin were detected by western blot. Results: The IC 50 values of DMY on KYSE410 and KYSE150 cells were 100.51 and 101.27 μmol/L. The clone formation numbers of KYSE150 and KYSE410 in DMY group [(0.53±0.03) and (0.31±0.03)] were lower than those in DMSO group [(1.00±0.10) and (1.00±0.05), P <0.05]. The apoptosis rates of KYSE150 and KYSE410 cells in DMY group [(1.84±0.22)% and (2.80±0.07)%] were higher than those in DMSO group [(1.00±0.18)% and (1.00±0.07)%, P <0.05]. The invasion numbers of KYSE150 and KYSE410 cells in DMY group [(0.42±0.03) and (0.29±0.05)] were lower than those in DMSO group [(1.00±0.08) and (1.00±0.05), P <0.05]. The migration rates of KYSE150 and KYSE410 cells in DMY group [(0.65±0.14)% and (0.40±0.17)%] were lower than those in DMSO group [(1.00±0.10)% and (1.00±0.08)%, P <0.05]. The clone formation numbers of KYSE150 and KYSE410 in TGF-β1 group [(1.01±0.08) and (0.99±0.25)] were higher than those in DMY+ TGF-β1 group [(0.73±0.10) and (0.58±0.05), P <0.05]. The apoptosis rates of KYSE150 and KYSE410 cells in TGF-β1 group [(0.81±0.14)% and (1.18±0.10)%] were lower than those in DMY+ TGF-β1 group [(1.38±0.22)% and (1.85±0.04)%, P <0.05]. The invasion numbers of KYSE150 and KYSE410 cells in TGF-β1 group [(1.19±0.11) and (1.39±0.11)] were higher than those in DMY+ TGF-β1 group [(0.93±0.09) and (0.93±0.05), P <0.05]. The migration rates of KYSE150 and KYSE410 cells in TGF-β1 group [(1.87±0.19)% and (1.32±0.04)%] were higher than those in DMY+ TGF-β1 group [(0.86±0.16)% and (0.77±0.12)%, P <0.05]. The protein expression levels of Bax, Caspase-3 and Caspase-9 in KYSE150 and KYSE410 cells in DMY group were higher than those in DMSO group, while the protein expression level of Bcl-2 was lower than that in DMSO group ( P <0.05). The protein expression levels of p-Smad2/3, Smad2/3 and Vimentin in KYSE150 and KYSE410 cells in DMY group were lower than those in DMSO group ( P <0.05). The protein expression levels of Bax, Caspase-3 and Caspase-9 in KYSE150 and KYSE410 cells in TGF-β1 group were lower than those in DMY+ TGF-β1 group, and the protein expression level of Bcl-2 was higher than that in DMY+ TGF-β1 group ( P <0.05). The protein expression levels of Bax, Caspase-3 and Caspase-9 in KYSE150 and KYSE410 cells in DMY+ TGF-β1 group were lower than those in DMY group, and the protein expression level of Bcl-2 was higher than that in DMY group ( P <0.05). The protein expression levels of p-Smad2/3, Smad2/3 and Vimentin in KYSE150 and KYSE410 cells in TGF-β1 group were higher than those in DMY+ TGF-β1 group ( P <0.05). Conclusion: DMY can inhibit the proliferation and EMT of ESCC mediated by TGF-β1 and promote cell apoptosis.

Objective: To explore the mechanism underlying the hepatoprotective effect of dihydromyricetin (DMY) against lipid accumulation in light of the lipophagy pathway and the inhibitory effect of DMY on HepG2 cell proliferation., Methods: LO2 cells were cultured in the presence of 10% FBS for 24 h and treated with 100 μg/mL DMY, or exposed to 50% FBS for 24 h followed by treatment with 50, 100, or 200 μg/mL DMY; the cells in recovery group were cultured in 50% FBS for 24 h and then in 10% FBS for another 24 h. Oil red O staining was used to observe the accumulation of lipid droplets in the cells, and the levels of TC, TG, and LDL and activities of AST, ALT and LDH were measured. The expression of LC3 protein was detected using Western blotting. AO staining and transmission electron microscopy were used to determine the numbers of autophagolysosomes and autophagosomes, respectively. The formation of autophagosomes was observed with MDC staining, and the mRNA expression levels of LC3, ATG7, AMPK, mTOR, p62 and Beclin1 were determined with q-PCR. Flow cytometry was performed to analyze the effect of 50, 100, and 200 μg/mL DMY on cell cycle and apoptosis of HepG2 cells; DNA integrity in the treated cells was examined with cell DNA fragmentation test., Results: DMY treatment and pretreatment obviously inhibited lipid accumulation and reduced the levels of TC, TG, LDL and enzyme activities of AST, ALT and LDH in LO2 cells ( P < 0.05). In routinely cultured LO2 cells, DMY significantly promoted the formation of autophagosomes and autophagolysosomes and upregulated the expression of LC3 protein. DMY obviously attenuated high FBS-induced inhibition of autophagosome formation in LO2 cells, up- regulated the mRNA levels of LC3, ATG7, Beclin1 and AMPK, and downregulated p62 and mTOR mRNA levels ( P < 0.05 or 0.01). In HepG2 cells, DMY caused obvious cell cycle arrest, inhibited cell proliferation, and induced late apoptosis and DNA fragmentation., Conclusion: DMY reduces lipid accumulation in LO2 cells by regulating the AMPK/ mTOR-mediated lipophagy pathway and inhibits the proliferation of HepG2 by causing cell cycle arrest and promoting apoptosis.

Objective: To investigate the inhibitory effects of dihydromyricetin on the proliferation and migration of gastric cancer BGC-823 cells and explore the molecular mechanisms., Methods: BGC-823 cells in routine culture were treated with different concentrations of dihydromyricetin (0, 40, 60, 80, 100, and 120 μg/mL) for 24 h, and the changes in cell viability were detected using CCK-8 assay; colony forming assay and Transwell assay were performed to assess the changes in colonyforming and migration abilities of the cells, respectively. The levels of MMP-2 and MMP-9 in the treated cells were determined using ELISA, and Western blotting was used to detect the expressions of E-cadherin, N-cadherin, cyclin D1, cyclin E1, HSP70 and HMGB1 and the phosphorylation levels of Akt and Stat3., Results: CCK-8 assay showed that dihydromyricetin treatment dose-dependently inhibited the viability of BGC-823 cells ( P < 0.05). Treatment with dihydromyricetin obviously suppressed the proliferation and migration of BGC-823 cells, significantly reduced the expression levels of cyclin D1, cyclin E1 and Ncadherin, enhanced E-cadherin expression, inhibited the phosphorylation of Akt and stat3, and downregulated HMGB1 expression in the cells. The results of ELISA demonstrated significantly lowered levels of MMP-2 and MMP-9 in dihydromyricetin-treated cells., Conclusions: Dihydromyricetin inhibits the proliferation and migration of BGC-823 cells through suppressing the activation of Akt/stat3 signaling pathways and HMGB1 expression.

Myricetin and its glycosides are important flavonols commonly found in plants, and they are natural organic compounds with diverse pharmacological activities. Numerous studies have demonstrated that myricetin and its glycosides are strong antioxidants that have great potential in preventing, alleviating and assisting the treatment of chronic non-infectious diseases such as cancer, diabetes, and cardiovascular diseases. In addition, myricetin and its glycosides also have antiviral, antibacterial, anti-inflammatory, analgesic, liver protection and other pharmacological activities. Myricetin contains more hydroxyl groups in the parent ring structure than other flavonoids, so myricetin and its glycosides have stronger pharmacological activities than other flavonols or flavonoids such as quercetin and kaempferol. Therefore, myricetin and its glycosides have great development and application prospects. In this paper, the classification and distribution of myricetin and its glycosides, their pharmacological activity and mechanism, as well as comparison with other flavonoids were reviewed. In addition, limitations of the current research and application of myricetin and its glycosides were analyzed, and the further studies on pharmacological activities as well as their dose-activity relationship, structure-activity relationship, chemical modification, biosynthesis and application prospects of myricetin and its glycosides were discussed and proposed.

In order to study the pathways of biosynthesis of flavonoids in Sophora japonica, 113 797 unigenes were obtained by Trinity software, with an average length of 803 bp, of which 72 752 unigenes were obtained from the database by high-throughput sequencing, and a total of 38 891 SSR loci were searched. Through the metabolic pathway analysis, we found that there were 135 unigenes involved in the biosynthesis of flavonoids and 959 unigene involved in other secondary metabolic pathways. Further analysis of genes involved in rutin biosynthesis revealed that 24 were associated with CHS, 52 were associated with FLS, and 11 were associated with UFGT. The obtained data of S. japonica transcriptome lays the foundation for studying the pathways of biosynthesis of flavonoids in S. japonica and provides theoretical basis for the formation of the quality of S. japonica., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Chinese Pharmaceutical Association.)

Objective: To explore the effects of fisetin and fisetin nanopartical on anti-proliferation and apoptosis induction of human ovarian cancer cell line SKOV3., Methods: The MTT assay was used to determine the survival rate of SKOV3 cell through apoptosis induction after fisetin and fisetin nanopartical treatment, and the drug concentration of the next experiment was selected. 15 nude mice vaccinated SKOV3 cells mixture mode randomly into three groups, treatment began on the fifth day, every 3 d injection times, each time 0.2 mL, a total of 12 times. Group A for the control (50 mL/L glucose solution), group B for fisetin (1.25 mg/kg), group C for fisetin nanopartical (1.25 mg/kg). After the treatment, tumor quality was recorded, and histopathological lesions were observed by HE staining on heart, liver, spleen, lung and kidney sections. The proliferation of SKOV3 cells was detected by Ki67 immunohistochemical staining in tumor tissue sections, and apoptosis of SKOV3 cells was detected by TUNEL assay., Results: MTT test indicated that fisetin and fisetin nanopartical had inhibition effect on ovarian cancer cell in a dose-dependent manner. The half-maximal inhibitory concentration (IC 50 ) value for fisetin and fisetin nanopartical were 125-250 μg/mL and 62.5-125 μg/mL. Therefore, the working concentration was selected as 125 μg/mL. 11 mice were completed and survived until the end of the experiment ( n= 3 in group A, n= 4 in group B, n= 4 in group C). Group A nude mice abdominal cavity in cauliflower shape tumor nodules, B and C group only tiny granule tumor nodules, B and C group of intraperitoneal tumor mass is quite ( P >0.05), but were lower than group A ( P <0.05). No obvious damage was found in the hepatocytes, liver, spleen, lung, and kidney slices. Compared with group A, Ki67 index in group B and group C decreased, and cell apoptosis rate increased ( P <0.05), but there was no significant difference between group B and group C ( P >0.05).After treatment of fisetin and fisetin nanopartical, heart, liver, spleen, lung and kidney are safe without damage., Conclusion: Fisetin and fisetin nanopartical have the effect of anti-ovarian cancer cells, and no organ damage has been found in both., (Copyright© by Editorial Board of Journal of Sichuan University (Medical Science Edition).)

Objective To investigate the protective effect of fisetin (FIS) against hypoxia/reoxygenation (H/R) injury in rat hepatocytes and its mechanism. Methods H/R injury model of BRL-3A cells was established and the cells were pretreated with FIS. Survival rate was detected by CCK-8 assay. Cell apoptosis was measured by flow cytometry. The levels of ALT and AST were determined by microplate assay. The production of TNF-α and IL-1β were detected by ELISA. The mRNA and protein levels of TLR4 and NF-κBp65 were analyzed by quantitative real-time PCR and Western blotting, respectively. Results After subjected to H/R, cell survival rate decreased and the apoptosis level increased. The levels of ALT and AST in cell supernatant were elevated, so were the production of TNF-α and IL-1β. FIS pretreatment increased the cell survival rate and inhibited apoptosis. The levels of ALT, AST and the production of TNF-α and IL-1β were reduced significantly. Moreover, FIS inhibited the increasing expression levels of TLR4 and NF-κBp65 induced by H/R. Conclusion FIS alleviates the hepatocyte injury induced by H/R via modulation of TLR4/NF-κB signaling pathway.

Objectives: We attempted to survey the inhibit effect of fisetin with human ovarian cancer cell line SKOV3 and the xenograft and the mechanism of the effect., Methods: The ovarian cancer cell line SKOV3 treated by fisetin were observed directly under the transmission electronmicroscope (TEM);MTT assay was used to determine cell viability.Flow cytometry was used to analyze the apoptosis in ovarian cancer cell line SKOV3.In addition,we established an ovarian cancer athymicnude rat model.We observed the neoplasia and progression after fisetin treatment.The proliferation and apoptosis of athymic nude rat model were evaluated by testing Bcl-2,Bax and poly-ADP-ribose polyerase (PARP) expression through Western blot., Results: The chromatin were brought together and the apoptotic bodies were detected in SKOV3 cells under transmission electron microscope after the treatment by fisetin.MTT assay indicated that fisetin inhibited ovarian cancer cell proliferation in a dose-dependent manner.The flow cytometry data demonstrated that the apoptosis might induct in SKOV3 cells after treatment by fisetin.In athymic rude rat model,under the influence of fisetin,tumor volume and tumor mass were significantly decreased.Western blot demonstrated that treatment with higher concentration of fisetin resulted in a significant decrease of Bcl-2 and a significant increase of Bax.The apoptosis proteins PARP was cut apparently., Conclusions: The results provided the first insight into antitumor anti-proliferative and the induction of apoptosis efficacy of fisetin against ovarian cancer in vitro and in vivo .All data suggested a safe promising therapeutic potential of fisetin in ovarian cancer treatment.

Objective: To investigate the reversal effect of dihydromyricetin(DMY) on drug resistance of K562/A02 cells to adriamycin and explore its possible mechanism., Methods: K562 and K562/A02 cells were treated with DMY (5, 10, 20, 40, 60, 80 and 100 mg/L) and ADM (100-0.05 mg/L) for 48 h. The viability of K562 cells and K562/A02 cells was tested and the reversal effect of DMY on drug resistance of K562/A02 cells to adriamycin was analyzed by MTT. The relative concentration of ADM in cells was measured by flow cytometry. Protein expressions of drug resistance related genes including P-glycoprotein (P-gp), multidrug resistance associated protein 1 (MRP1), glutathione transferase π (GSTπ) and BCL-2 were measured by Western Blot., Results: The proliferation of K562 and K562/A02 cells was significantly decreased by DMY in dose-dependent manner as compared with control group (r1=0.37, r2=0.38). The IC 50 of ADM on K562 and K562/A02 cells were 71.23±6.51 and 72.88±5.49 mg/L respectively. DMY (5, 10 and 20 mg/L) was low cytotoxicity. DMY (5, 10 and 20 mg/L) enhanced the sensitivity of K562/A02 cells to ADM in dose-dependent manner (r1=-0.62, r2=-0.71) and the reversal multiples was from 1.38 to 28.591. The relative concentrations of ADM in K562/A02 of DMY (5, 10 and 20 mg/L) group cells were significantly increased in dose-dependent manner compared with the control group (r=0.34). Compared with the control group, the expressions of drug resistance related protein P-gp, MRP1, GSTπ and BCL-2 were significantly decreased in dose-dependent manner in DMY (5, 10 and 20 mg/L) group (r1=-0.41, r2=-0.37, r3=-0.58, r=-0.46). Compared with the ADM group, the protein expressions of drug resistance related genes P-gp, MRP1, GSTπ and BCL-2 in DMY (5, 10 and 20 mg/L)+ADM group were significantly decreased in dose-dependent manner (r1=-0.55, r2=-0.41, r3 =-0.38, r4=-0.44)., Conclusion: DMY enhances the sensitivity of K562/A02 cells to ADM, its mechanism may be related with decrease of P-gp, MRP1, GSTπ and BCL-2 expressions.