基于代谢组学分析黑老虎植株不同部位黄酮类成分.

The root of Kadsura coccinea is a commonly used herb in traditional Chinese medicine, and flavonoids are one of the main active constituents of medicinal plants. This study intended to investigate whether the leaves and stems of K. coccinea plants may contain similar or highly enriched flavonoids in roots and to explore its application value. Widely-targeted metabolomics was used to identify the compounds in different organs of K. coccinea. According to their structural distribution and categories, the diversity and abundance of flavonoids were analyzed. The results were as follows:(1)The number of flavonoids metabolites in K. coccinea was sorted in descending order as leaf(80)> stem(73)> root(67), and a total of 61 flavonoids were the same in three parts. Leaves and stems contained more flavonols, contributing to a diversity of flavonoids greater than that in the roots.(2)Flavonols, flavonoids, flavanols, and chalcones were highly accumulated in the three parts. Among them, the accumulation of flavonols and flavonoids was lower in the stems and roots, resulting in a continuously, substantially decreasing abundance of flavonoids from leaf(24.00 × 107)to stem(13.45 × 107)to root(9.05 × 107).(3)Leaves and stems of K. coccinea contained a large amount of flavonoids that were also found in the roots and a variety of those not found in the roots, which could be considered for alternative or complementary use. Catechins highly expressed in all three parts, and the leaves were also rich in quercetin and its derivatives, which were highly valuable in application. [ABSTRACT FROM AUTHOR]