1. Brucella abortus Omp19 recombinant protein subcutaneously co-delivered with an antigen enhances antigen-specific T helper 1 memory responses and induces protection against parasite challenge.
- Author
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Coria LM, Ibañez AE, Pasquevich KA, Cobiello PLG, Frank FM, Giambartolomei GH, and Cassataro J
- Subjects
- Animals, Antibodies, Bacterial blood, Antigens, Protozoan immunology, Brucella abortus, Cattle, Cells, Cultured, Dendritic Cells immunology, Female, Immunologic Memory, Lymphocyte Activation, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Ovalbumin administration & dosage, Recombinant Proteins immunology, Trypanosoma cruzi, Adjuvants, Immunologic administration & dosage, Antigens, Bacterial immunology, Bacterial Outer Membrane Proteins immunology, Immunity, Cellular, Lipoproteins immunology, Th1 Cells immunology
- Abstract
The discovery of effective adjuvants for many vaccines especially those with limited commercial appeal, such as vaccines to poverty-related diseases, is required. In this work, we demonstrated that subcutaneous co-administration of mice with the outer membrane protein U-Omp19 from Brucella spp. plus OVA as antigen (Ag) increases Ag-specific T cell proliferation and T helper (Th) 1 immune responses in vitro and in vivo. U-Omp19 treated dendritic cells promote IFN-γ production by specific CD4(+) T cells and increases T cell proliferation. U-Omp19 co-administration induces the production of Ag specific effector memory T cell populations (CD4(+) CD44(high) CD62L(low) T cells). Finally, subcutaneous co-administration of U-Omp19 with Trypanosoma cruzi Ags confers protection against virulent parasite challenge, reducing parasitemia and weight loss while increasing mice survival. These results indicate that the bacterial protein U-Omp19 when delivered subcutaneously could be a suitable component of vaccine formulations against infectious diseases requiring Th1 immune responses., (Copyright © 2015 Elsevier Ltd. All rights reserved.)
- Published
- 2016
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