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Immunogenicity of the Brucella melitensis recombinant ribosome recycling factor-homologous protein and its cDNA.
- Source :
-
Vaccine [Vaccine] 2002 Feb 22; Vol. 20 (11-12), pp. 1660-9. - Publication Year :
- 2002
-
Abstract
- A study was conducted to evaluate the immunogenicity of the Brucella melitensis ribosome recycling factor (RRF)-homologous protein (CP24). The CP24 gene was cloned, expressed in Escherichia coli and purified. The resulting purified recombinant protein (rCP24) produced delayed-type hypersensitivity (DTH) reactions in B. melitensis-infected mice but not in naive controls. Thus, we decided to characterise the immune responses generated with DNA vaccination (pcDNACP24) or immunisation with the rCP24 in adjuvant. Animals injected with pcDNACP24 exhibited a dominance of IgG2a to IgG1 while mice injected with rCP24 developed a higher response of IgG1 than IgG2a. Both immunisation protocols were capable of eliciting CP24-specific gamma interferon (IFN-gamma) producing cells. Spleen cells from pcDNACP24-immunised mice did not produce interleukin (IL)-4, IL-10 or up-regulation of IL-2 mRNA. Cells from rCP24-immunised mice produced IL-10, up-regulated IL-2 mRNA but did not produce IL-4. Neither immunisation with purified CP24 nor injection of pcDNACP24 protected mice against challenge with live smooth B. melitensis. However, the potential of CP24 for a Brucella diagnostic test based on an in vitro antigen (Ag)-specific IFN-gamma production or DTH test would be worth testing.
- Subjects :
- Animals
Antibodies, Bacterial biosynthesis
Base Sequence
Brucella Vaccine genetics
COS Cells
Cloning, Molecular
Cytokines biosynthesis
Cytokines genetics
DNA, Bacterial genetics
DNA, Complementary genetics
Escherichia coli genetics
Female
Gene Expression
Genes, Bacterial
Hypersensitivity, Delayed
Immunization
Immunoglobulin G biosynthesis
Lymphocyte Activation
Mice
Mice, Inbred BALB C
Recombinant Proteins genetics
Recombinant Proteins immunology
T-Lymphocytes immunology
Transfection
Vaccines, DNA genetics
Vaccines, DNA immunology
Vaccines, Synthetic genetics
Vaccines, Synthetic immunology
Bacterial Proteins genetics
Bacterial Proteins immunology
Brucella Vaccine immunology
Brucella melitensis genetics
Brucella melitensis immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0264-410X
- Volume :
- 20
- Issue :
- 11-12
- Database :
- MEDLINE
- Journal :
- Vaccine
- Publication Type :
- Academic Journal
- Accession number :
- 11858876
- Full Text :
- https://doi.org/10.1016/s0264-410x(01)00498-4