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Brucella abortus Omp19 recombinant protein subcutaneously co-delivered with an antigen enhances antigen-specific T helper 1 memory responses and induces protection against parasite challenge.
- Source :
-
Vaccine [Vaccine] 2016 Jan 20; Vol. 34 (4), pp. 430-437. Date of Electronic Publication: 2015 Dec 17. - Publication Year :
- 2016
-
Abstract
- The discovery of effective adjuvants for many vaccines especially those with limited commercial appeal, such as vaccines to poverty-related diseases, is required. In this work, we demonstrated that subcutaneous co-administration of mice with the outer membrane protein U-Omp19 from Brucella spp. plus OVA as antigen (Ag) increases Ag-specific T cell proliferation and T helper (Th) 1 immune responses in vitro and in vivo. U-Omp19 treated dendritic cells promote IFN-γ production by specific CD4(+) T cells and increases T cell proliferation. U-Omp19 co-administration induces the production of Ag specific effector memory T cell populations (CD4(+) CD44(high) CD62L(low) T cells). Finally, subcutaneous co-administration of U-Omp19 with Trypanosoma cruzi Ags confers protection against virulent parasite challenge, reducing parasitemia and weight loss while increasing mice survival. These results indicate that the bacterial protein U-Omp19 when delivered subcutaneously could be a suitable component of vaccine formulations against infectious diseases requiring Th1 immune responses.<br /> (Copyright © 2015 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Antibodies, Bacterial blood
Antigens, Protozoan immunology
Brucella abortus
Cattle
Cells, Cultured
Dendritic Cells immunology
Female
Immunologic Memory
Lymphocyte Activation
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Ovalbumin administration & dosage
Recombinant Proteins immunology
Trypanosoma cruzi
Adjuvants, Immunologic administration & dosage
Antigens, Bacterial immunology
Bacterial Outer Membrane Proteins immunology
Immunity, Cellular
Lipoproteins immunology
Th1 Cells immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2518
- Volume :
- 34
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Vaccine
- Publication Type :
- Academic Journal
- Accession number :
- 26707377
- Full Text :
- https://doi.org/10.1016/j.vaccine.2015.12.012