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【Objective】 This study analyzed the microbial community composition and variation of Leersia hexandra Swartz ecological floating bed in the process of purifying wastewater. 【Method】 High throughput Illumina Miseq was used to analyze the microbial community composition and variation in the process of purifying wastewater. 【Result】 Results showed that the microbial diversity and community composition changed with time, and the dominant microbial groups and relative abundances were different. Planting Leersia hexandra Swartz treatment increased wastewater microbial diversity with time. Proteobacteria was predominant microbes in the four treatments. The relative abundance of Bacteroidetes increased and Firmicutes decreased under planting Leersia hexandra Swartz and planting Leersia hexandra Swartz with aerobic treatments. Flavobacterium, Hydrogenophaga and Rhodobacter were the dominant genus in the four treatments. The planting Leersia hexandra Swartz with aerobic treatment was profitable for growth and propagation of carbon cycle related microbes such as sediminibacterium, Emticicia, Novosphingobium, Luteolibacter, Comamonadaceae and Erythrobacteraceae. For planting Leersia hexandra Swartz with anaerobic treatment, the phosphorus solubilizing microbes such as Pseudomonas, Flavobacterium and Rhodobacter were enriched. 【Conclusion】 The microbial diversity and community composition was no obvious relation with wastewater purification. The cluster and PCOA analyses indicated the microbial compositions clustered together for planting Leersia hexandra Swartz with anaerobic treatment and static wastewater control, and which were similar for planting Leersia hexandra Swartz and planting Leersia hexandra Swartz with aerobic treatment. The planting Leersia hexandra Swartz with aerobic treatment would be helpful to remove COD. The planting Leersia hexandra Swartz with anaerobic treatment was helpful to phosphorus degradation. [ABSTRACT FROM AUTHOR]

[Objective]To discover the optimum mung bean interplanting and straw returning time, the fertility effects of sugarcane/mung bean intercropping mode and mung bean straw returning were studied. [Method] A field experiment designed with split plot of 2 factors was conducted = aiming at revealing the effect of different treatments on agricultural characters, yield, and quality of sugarcane. [Result] Results showed that highest dry matter and N, P, K nutrient accumulation were showed in treatment of intercropping(sowing mung bean in sowing time of sugarcane + returning mung bean straw in fruiting period). After mung bean straw returning, the degradation rates of nitrogen, phosphorus, potassium were basically the same between the mode ol straw aboveground covering in early period and the mode ol straw underground covering. But the degradation rate between these two modes reversed in later period. Intercropping of sugarcane and mung bean brought about some negative effects on sugarcane emergence and tillering, the earlier the mung bean intercropped, the greater the negative effects. Height, cane diameter and single stem weight can be promoted by mung bean interplanting and its straw returning. The treatment of intercropping(sowing mung bean in sowing time of sugarcane + returning mung bean straw in fruiting period) presented highest cane production which was obviously higher than other treatment. There are no notorious differences of sugar brix in sugarcane juice between treatments of intercropping and noninlercropping. [Conclusion ] Intercropping of sugarcane and mung bean should be vigorously promoted. Il can promote soil fertility and sugarcane production without reducing sugar content. The best treatment is to intercrop mung bean in sowing time of sugarcane and return mung bean straw with aboveground covering mode in Iruiting period ol mung bean. [ABSTRACT FROM AUTHOR]

SoCYT cDNA was amplified using RT-PCR from sugarcane leaves, the characteristics of the deduced protein were analyzed using bioinformatics software and its expression was analyzed using quantitative real-time PCR. The results showed that the sequence contained a 796 bp full length fragment with a 579 bp open reading frame, and it encoded a putative SoCYT protein with 192 amino acids. Sequences alignment showed that the homology of cDNA sequence between sugarcane and Sorghum bicolor SoCYT gene was 94% , while the homology of amino acid sequence between them was 99%. The relative protein molecular weight was 20. 67 kD, the PI was 6.24, and the hydrophobic value was from -0.5 to 2.11. The molecular structure prediction results suggested that the protein contained 17.19%, -helix, 53.12% loop, 21.53% extended strand and 8.33% + meander. The sequence has been submitted to GenBank with an accession number of JQ712582. The results of quantitative real-time PCR analysis showed that the mRNA of SoCYT was decreased with time of PEG stress, significant differences were observed between the treatments of PEG and PEG + Si at 18 , 26, 40 , 64, 96, 144 h of the treatment. Silicon can slowdown the damage and effect of drought on the structure of the cytochrome b6/f complex. [ABSTRACT FROM AUTHOR]

Vietnam is located in the tropics, is rich in sunlight and rainfall, especially good for sugarcane growth. Sugarcane is the most important sugar crop in Vietnam, and there are 300,000 hm2 of sugarcane planting area which produces a total of more than 20 million tons of millable cane each year. Based on field investigation and the statistical data from the Vietnam Agricultural and Rural Departments and the Statistical Yearbook of Vietnam, the sugarcane growing area, cane yield and total sugarcane production in different agro-ecological zones and some provinces in Vietnam during recent 6-10 years were compared and analyzed to provide references for sugarcane researchers, managers and the authorities in China. [ABSTRACT FROM AUTHOR]

【Objective】The present paper aimed to analyze the effects of pesticides fertilizer application on the occurrence of diseases and insect pests in sugarcane during inter-tillage period, and their production effects at jointing and maturity stages, so as to provide reference basis for scientific application of pesticide fertilizer in sugarcane.【Method】In this study, sugarcane cultivar Guiliu 05/136 planted in winter was taken as the research material. Referring to the production standard of sugarcane with high yield and high sugar in Guangxi, six different kinds of pesticides fertilizers with nutrient ratios and pesticides components were applied to sugarcane production during the inter-tillage period（from tillering stage to late tillering stage） as different treatments, and they were named as A,B,C,D,E and F,respectively. From May to October, the conditions of major plant diseases and insect pests in sugarcane seedlings under different treatments, and the growth rate of sugarcane plants at elongation stage were investigated. The yield traits were investigated at maturity, and the agricultural output value of sugarcane under different treatments were also analyzed.【Result】Application of pesticides fertilizer with N, P2O5, K2O≥30%（A,B,C,D and E treatments） promoted sugarcane tillering, appropriately applying with organic matter in which（B and D treatments） had better effect. At the same time, the smut and borer pest also got better control in sugarcane seedling. The pesticides fertilizer containing organic matter had a better indirect effect on smut, and which containing chlorantraniliprole and thiamethoxam could prevent and control borers in best. In addition, the application of pesticides fertilizers containing organic matter（B,D and F treatments） could not only promote the elongation of sugarcane stem in advance, but also promote rapidly growth for plants, and to form more millable canes, then increase their yield per unit area. Under the premise of the same cost of fertilizer, the agricultural increased of sugarcane treated with different fertilizer reached by 2118.62-9199.31 yuan/hm~2, among which D treatment had the highest value added.【Conclusion】The application of organic-inorganic compound pesticides fertilizer during the inter-tillage period have positive effect on the control of diseases and insect pests of sugarcane seedlings, and the control effect is closely related to the content of medicinal ingredients and nutrient ratio of fertilizer. The tillering of sugarcane can be effectively promoted by applying medicated fertilizer, which could also effectively improve the effect for sugarcane production, and two kinds of which the component of 35% N, P2O5, K2O content（17-7-11）,0.10% insecticide, 0.10% thiamethoxam, 3% organic matter or 30% N, P2O5, K2O content（13-6-11）,30 mL Durui, 0.10% thiamethoxam, 15% organic matter are more beneficial to increase sugarcane production efficiency, thus it is the best for sugarcane cultivation in Guangxi. [ABSTRACT FROM AUTHOR]

【Objective】The present experiment aimed to identify the pathogen species of top blight of Passiflora edulis and clarify its taxonomic status, so as to provide a theoretical basis for the scientific prevention and control of top blight of P. edulis and the sustainable and rapid development of the P. edulis industry.【Method】The pathogen of P. edulis top blight, a new disease found in Nanning for the first time, was isolated by plant tissue isolation method, its pathogenicity was determined, and its morphological characteristics were observed, and the genetic sequence of its ribosomal transcribed spacer ( ITS),calmodulin (CAL) and mating type gene (ApMat) were analyzed. To identify the pathogen causing top blight of P. edulis.【Result】The same form of fungi was isolated from the plants with typical symptoms of top blight of P.caerulea, named TK2 A2 CLA6 strain, and its spore morphology was consistent with the pathogen spore morphology observed from the slices of natural diseased branches of P.caerulea, which could be infected by the pathogenicity test of terminal bud inoculation, and the disease symptoms were consistent with the natural disease symptoms. The pathogenic bacteria obtained by re-separation were the same as the original inoculated pathogenic bacteria. The colony of TK2 A2 CLA6 was round or nearly round, and developed and dense gray-white aerial hyphae were produced on the front of the colony, while the back of the colony was light yellow at the early stage. At the later stage, the center of the fungus was light black and the periphery was light yellow, and orange spore piles were occasionally produced after 7 days of culture on PDA medium. 18.89 mm/d; Colorless conidia was nearly cylindrical and obtuse, the other end was obtuse or slightly thin, generally with 1-2 oil globules, the size was (13.03-17.75) μm× (3.75-5.25) μm. The genetic sequence of TK2 A2 CLA6 strain was amplified by PCR,and the genetic sequence was analyzed by BLAST in NCBI. The results showed that the homology between TK2 A2 CLA6 strain and Colletotrichum siamense was 100%. The phylogenetic tree of multi-gene phylogeny was analyzed by its, CAL and ApMat genetic sequence. Strain TK2 A2 CLA6,C. siamense LF177 and C. siamense JH-Coll001 were clustered into an independent branch with 100% self-supporting rate, and the pathogen causing top blight of P. edulis was identified as C. siamense.【Conclusion】 The pathogen of P. edulis edulis in Nanning, Guangxi was identified as C. siamensis by morphological method and multi-gene phylogenetic tree analysis. [ABSTRACT FROM AUTHOR]

[Objective]The present paper aims to compare the nucleosides contents between wild and cultivated Paris polyphylla Smith var. yunnanensis from different regions, and study whether the wild and cultivated varieties had quality equivalence. [Method]The content of 9 nucleosides in 16 wild varieties and 17 cultivated varieties was determined by HPLC, and evaluated by independent sample t test, cluster analysis and principal component analysis. [Result]The results of independent sample t test showed that there were significant differences in the contents of cytidine, thymidine and 2’-deoxyadenosine between the wild and cultivated varieties(Sig.<0.05), while there were no differences in the contents of the other six nucleosides(Sig.>0.05). The results of principal component analysis and cluster analysis showed that the wild and cultivated varieties could not be distinguished according to the difference of nucleoside content. [Conclusion]There was no significant difference in nucleoside content between wild and cultivated Paris polyphylla. And the cultivated Paris polyphylla could replace the wild Paris polyphylla. [ABSTRACT FROM AUTHOR]

【Objective】 To provide reference basis for parents selection and matching of sugarcane breeding, the flowering habits of 12 newly introduced sugarcane parents from America were clarified. 【Method】 The initial flowering time, heading rate and pollen dyeing rate were investigated and analyzed in continuous 5 crossing seasons from 2013/14 to 2017/18. 【Result】 Broad-sense heritability of the initial flowering time, the heading rate and the pollen dyeing rate was 0.9518, 0.7961 and 0.9384, respectively. The parents with the earliest first flowering time were CP07-1618 and CP09-4448, and the parent with the latest first flowering time was CP07-2137. The latest flowering time for the 12 parents was in 2015/16 crossing season. There was no significant difference in initial flowering time among the parents in other crossing seasons. The parents with low heading rate were CP01-2390, CP07-2137 and CP09-4707, belonging to difficult flowering parents, and the rest of 9 parents were easy flowering parents. The parents with small pollen dyeing rates were CP00-1630, CP06-3458, CP07-1618, CP07-2137, which could only be used as female parents because their pollen dyeing rates were no more than 30% in all crossing seasons. The parents with high pollen rate were CP06-2422 and CP09-4448. 【Conclusion】 The broad-sense heritability characters of the initial flowering time, heading rate and pollen dyeing rate of sugarcane parents were high heritability, so the 12 parents could be selected strictly in the stage of crossing combination. [ABSTRACT FROM AUTHOR]

【Objective】The objective of this study is to clone the full-length cDNA sequence of invertase inhibitor gene(SoInv Inh2) from sugarcane,and analyze its sequence and expression patterns for further identifying the biological functions of SoInvInh2. 【Method】The 5(partial cDNA sequences of SoInv Inh2 gene was cloned from sugarcane immature internodes using homologous sequence, and 3(partial cDNA sequences was cloned through Tail-PCR(Thermal asymmetric interlaced PCR). Bioinformatic method was deployed to analyze the deduced amino acid sequence of SoInv Inh2 and the physicochemical properties, subcellular localization of SoInvInh2. Phylogenetic tree was also constructed. The spatial-specific expression of SoInvInh2 gene were determined by real-time fluorescence quantitative PCR(qRT-PCR) in different tissue at different growth stage of sugarcane. 【Result】A novel InvInh gene was cloned from the sugarcane immature internodes, which was named as SoInv Inh2 gene with the GenBank accession number KF575170. The full-length cDNA of SoInvInh2 was 927 bp, containing a651 bp open reading frame(ORF) which encodes 216 amino acids. There was no intron in SoInv Inh2. The SoInvInh2 protein contains four asymmetric α-helixes and four conservative Cys sites and belongs to the InvI/PMEI(Invertase and pectin methylesterase inhibitors)family. The results of qRT-PCR showed that SoInv Inh2 could be detected in the leaves, internodes, rachis and inflorescence. At different growth stages of sugarcane,the expression pattern of SoInv Inh2 gene in leaves and stems of different maturity had no obvious regularity. 【Conclusion】The successful isolation and bioinformatics analysis of SoInv Inh2 gene will lay the foundation for a further study. [ABSTRACT FROM AUTHOR]

[Objective]To provide experimental basis for further research on regulation mechanism of ASR in response to drought, ASR (ABA-stress-ripening) gene from sugarcane was cloned and its expression level under drought stress was detected. [Method] Based on the total RNA of sugarcane leaves, the cDNA of ASR gene was amplified by RT-PCR. the characteristics of the deduced protein were analyzed by using bioinformatics software and its expression was analyzed by quantitative real-time PGR. [Result] The results showed that the cDNA of ASR gene which obtained by RT-PGR, was 402 bp in full length, and it encoded a putative ASR protein with 133 amino acids, named SoASRl . its Gen Bank accession number was JQ712581. Comparison of the amino acids sequences homology in SoASRl protein from 12 different species indicated that the ASR protein had 56 % to 99 % identity in amino acids sequence with other plants. The results of quantitative realtime PGR analysis showed that the mRNA of SoASRl was increased initially and then decreased with extension of time under drought stress. The peak value of SoASRl gene expression in sugarcane leaves treated with PEG and Si was 34 h earlier than that of drought treatment. [Conclusion] SoASRl was induced by drought stress and was involved in drought-resistant reaction at transcriptional level, so it could be served as a candidate gene for further study of drought-resistant mechanism of sugarcane, which could be enhanced by Si application. [ABSTRACT FROM AUTHOR]

[Objective} In order to provide theoretical reference for optimizing methods of sugarcane mechanized cultivation and suitable varie­ties evaluation, the experiment was conducted to study the effect of mechanical compaction on seedling emergence and roots formation of ra­toon sugarcane. [Method]The randomized block designed with two factors,one factor contained the mechanical compaction treatments and the control, and the other factor was consist of different varieties, which were ROC22 . (7142. (7143 , (TOO, (7103-2287 and (7198-296. Agro­nomic characters and functional properties parameters of roots were investigated at post-harvest. [Result] After mechanical roller compaction, the increase amplitude in soil bulk density was the highest at 10 -20 cm depth, and soil compaction augmented with the increase of soil depth. The soil pressure of the treatment and the control tended to be consistent when the soil depth reached 40 cm. The number of seedling emergence and the plant height in GT42. GT43 and GT98-296 were higher than the other tested varieties. The dry weight, the length, the superficial area and the mean diameter of roots decreased in different degrees, respectively. The change range of die dry weight of roots was negative correlation with its numerical value. Though the proportion of die second order roots, die diinl order roots and the fourth order roots decreased in different degrees, respectively, the proportion of the first order roots increased in all tested varieties. [Conclusion ] The soil compaction increased after mechanical roller compaction, which limited the growth of mots and affected the growth of ratoon sugarcane. The ability of different varieties to adapt soil com­paction stress was different. The varieties with strong ratoon seedling emergence ability and developed roots system should be selected when large harvest machines were used in sugarcane production. [ABSTRACT FROM AUTHOR]

The aim of this work was to screen out and identify a valuable resource for the study of biological fertilizer, and provide evidences for the exploration and utilization of associative nitrogen fixation bacteria in rhizosphere of sugarcane. This study exploited the conventional methods of separation of rhizosphere bacteria GG08160 from sugarcane, and the strain was found to have N2 fixing ability as demonstrated by the acetylene reduction assay and nifH gene amplification and sequence analysis, and biological functions of the strain including IAA secretion, phosphate solubilization, glyphosate-resistance were studied by Salkowski colorimetry, phosphorus hole (D/d ratio) and by gradient dilution glyphosate selected culture. Identification of the strain species according to the microscopic observation and 16 S rDNA sequence analysis results. A high glyphosate-resistance bacterium strain GG08160 was isolated from rhizosphere of sugarcane, which can grow well in glyphosate solution with the concentration of 250 mM. Also, the strain possessed the capacity of nitrogen fixation, IAA secretion and phosphate solubilization. Inoculation test showed that the train GG08160 can remarkably promote the growth of sugarcane plantlets. Based on phenotypic characteristics, biolog system test, and 16S rRNA sequence analysis, the strain GG08160 was identified as Klebsiella sp. [ABSTRACT FROM AUTHOR]

Stem disc of Zhongshui garlic were used as explants to study the effect of different combinations of hormone on the tissue culture. The results showed that the optimum medium for inducing adventitious bud from the stem disc of Zhong shui garlic was B5 + 1 mg/L 6-BA +0. 5 mg/L NAA; The optimum medium for induction of callus was B5 + 0. 5 mg/L 2. 4-D + 2 mg/L NAA + 1 mg/L 6-BA; Cytokinin at high concentration was favorable to the redifferentiation of callus, 6-BA was favorable to the induction of adventitious bud, KT was favorable to the induction of somatic embryos induction; the optimum medium for inducing root was 1/2B5 + 0. 1 mg/L IBA. [ABSTRACT FROM AUTHOR]

The paper focused on assess the genetic variability and diversity of water chestnut (Eleocharis tuberosa Schulut). Genetic relation among 24 cultivars of water chestnut was analyzed by inter-simple sequence repeat (ISSR) makers. 11 polymorphism primers were selected from 100 ISSR primers. A total of 86 ISSR bands were obtained, that is, 7.8 polymorphic ban each primer pair, and 69 of them were polymorphic, the polymorphism ratio was 80.3 %. The dissimilarity coefficient of the 24 water chestnut cultivars ranged from 0.0909 to 0.6071, with an average of 0.3493. The 24 water chestnut cultivars were divided into 5 groups at the genetic distance 0. 34 by UPGMA. The results showed that narrow genetic diversity and close genetic relationship presented among 24 water chestnut cultivars, and the difference varied in part of those cultivar from different areas, and the same as RAPD analysis at prophase. [ABSTRACT FROM AUTHOR]