甘蔗ASR基因克隆及水分胁迫下的表达分析.

[Objective]To provide experimental basis for further research on regulation mechanism of ASR in response to drought, ASR (ABA-stress-ripening) gene from sugarcane was cloned and its expression level under drought stress was detected. [Method] Based on the total RNA of sugarcane leaves, the cDNA of ASR gene was amplified by RT-PCR. the characteristics of the deduced protein were analyzed by using bioinformatics software and its expression was analyzed by quantitative real-time PGR. [Result] The results showed that the cDNA of ASR gene which obtained by RT-PGR, was 402 bp in full length, and it encoded a putative ASR protein with 133 amino acids, named SoASRl . its Gen Bank accession number was JQ712581. Comparison of the amino acids sequences homology in SoASRl protein from 12 different species indicated that the ASR protein had 56 % to 99 % identity in amino acids sequence with other plants. The results of quantitative realtime PGR analysis showed that the mRNA of SoASRl was increased initially and then decreased with extension of time under drought stress. The peak value of SoASRl gene expression in sugarcane leaves treated with PEG and Si was 34 h earlier than that of drought treatment. [Conclusion] SoASRl was induced by drought stress and was involved in drought-resistant reaction at transcriptional level, so it could be served as a candidate gene for further study of drought-resistant mechanism of sugarcane, which could be enhanced by Si application. [ABSTRACT FROM AUTHOR]