甘蔗细胞色素b6-f复合体铁硫亚基(SoCYT)基因的克隆和表达分析.

SoCYT cDNA was amplified using RT-PCR from sugarcane leaves, the characteristics of the deduced protein were analyzed using bioinformatics software and its expression was analyzed using quantitative real-time PCR. The results showed that the sequence contained a 796 bp full length fragment with a 579 bp open reading frame, and it encoded a putative SoCYT protein with 192 amino acids. Sequences alignment showed that the homology of cDNA sequence between sugarcane and Sorghum bicolor SoCYT gene was 94% , while the homology of amino acid sequence between them was 99%. The relative protein molecular weight was 20. 67 kD, the PI was 6.24, and the hydrophobic value was from -0.5 to 2.11. The molecular structure prediction results suggested that the protein contained 17.19%, -helix, 53.12% loop, 21.53% extended strand and 8.33% + meander. The sequence has been submitted to GenBank with an accession number of JQ712582. The results of quantitative real-time PCR analysis showed that the mRNA of SoCYT was decreased with time of PEG stress, significant differences were observed between the treatments of PEG and PEG + Si at 18 , 26, 40 , 64, 96, 144 h of the treatment. Silicon can slowdown the damage and effect of drought on the structure of the cytochrome b6/f complex. [ABSTRACT FROM AUTHOR]