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Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To observe the changes of gut microbiota in children with drug-resistant epilepsy and to illustrate the relationship between intestinal flora and drug-resistant epilepsy. Methods Children with newly diagnosed epilepsy were grouped into the drug-resistant（seizure reduction ≤50%）group and drug-responsive（seizure reduction> 50%） group according to the effect of anti-epileptic treatment（6 months），and meanwhile，another 25 healthy children were selected as the control group. Stool specimens were collected from all children with epilepsy before and after treatment and from children in the healthy control group，and the microbial composition was analyzed by 16s ribosomal DNA highthroughput sequencing. Results There was a statistically significant difference in the diversity of gut microbiota between the epilepsy group and the control group（P<0. 05）. There was a statistically significant difference in the diversity of gut microbiota between the drug-resistant group and the drug-responsive group（P<0. 05）. The ratio of Firmicutes to Bacteroides in the drug-resistant group before treatment was significantly higher than those of the other groups（P<0. 05）. Before treatment，we compared the abundance of intestinal bacteria in children between the drug-resistant group and the control group and the drug-responsive group，the abundance of Clostridium and Cronobacter increased，and the abundance of Bacteroides and Bifidobacterium decreased（all P<0. 05）. Conclusion Children with drug-resistant epilepsy have intestinal flora imbalance，with increased diversity of intestinal flora and increased rare intestinal flora. [ABSTRACT FROM AUTHOR]

Objective To explore the risk factors that affect the prognosis of children with acute myeloid leukemia (cAML), to construct a prognostic nomogram model and further to verify the predictive value of this model. Methods The data of 1 068 children with cAML were downloaded from TARGET database, and 839 cases with complete data were included after screening. The clinicopathological features of the children were collected, including age, sex, race, survival status, white blood cell count, central nervous system leukemia, whether there were extramedullary invasion diseases such as green tumor (chloroma), the number of peripheral blood mother cells, remission status, whether there were fusion genes, the number of abnormal chromosomes and structural changes. Univariate Cox regression analysis was used to screen the factors related to prognosis, and multivariate Cox regression analysis was used to further screen independent prognostic factors. A nomogram model was established to predict the overall survival (OS) of 3, 5 and 7 years. The prediction accuracy and discriminant ability of the nomogram model were determined by the concordance index (C index), the calibration curve, and the area under the ROC curve (AUC). The stability of the nomogram model was verified by internal verification. Results Multivariate Cox regression analysis showed that the independent influencing factors of OS were white blood cell count at diagnosis, monosomy-7, t (6; 11) (q27; q23), t (10; 11) (p11. 2; q23), t (8; 21), inv (16), and NPM mutation, and the nomogram model was successfully constructed based on the above predictors. The C index of the nomogram model constructed by the training set was 0. 704 (95% CI was 0. 677-0. 731), and the AUC values for predicting 3-, 5- and 7-year survival rates were 0. 701, 0. 709 and 0. 708, respectively. The C index of the nomogram model constructed by the internal verification set was 0. 702 (95% CI was 0. 689-0. 715), and the AUC values for predicting 3-, 5- and 7-year survival rates were 0. 700, 0. 713 and 0. 713, respectively. The calibration curves of the training set and the verification set showed that the predicted risk was consistent with the actual disease risk. The risk score was calculated based on the regression coefficient of multivariate Cox regression analysis. The children in training set and internal verification set were divided into the high risk score group and low risk score group. The results of Kaplan-Meier survival analysis showed that the OS of the high risk score group was significantly lower than that of low risk score group. Conclusion The influencing factors for the prognosis of children with cAML are white blood cell count at diagnosis, monosomy-7, (t 6; 11) (q27; q23), (t 10; 11) (p11. 2; q23), (t 8; 21), inv (16), and NPM mutation; the prognostic nomogram model successfully constructed based on the above predictive factors can objectively and accurately predict the prognosis of children with cAML. [ABSTRACT FROM AUTHOR]

Objective To explore the protective effect and mechanism of icariin (ICA) on the liver function of rats with acute liver injury. Methods Forty SPF rats were randomly divided into four groups; the control group, model group, low-dose ICA group, and high-dose ICA group. The rats in the low-dose ICA and high-dose ICA group were injected intrap-eritoneally with ICA 30 and 80 mg/kg. The rats in the control group and model group were given the same amount of normal saline once daily for 7 days. Two hours after the last administration, the model group and the low-dose ICA group and high-dose ICA group were given 0. 35% CC14 to prepare a liver injury model. The control group was given the same amount of normal saline and was sacrificed 12 hours later. Rat femoral artery blood was obtained. The alanine aminotransferase (ALT) activity, aspartate transaminase (AST) activity, superoxide dismutase (SOD) activity, glutathione peroxidase (GSH-Px) activity and malondialdehyde (MDA) content in rats were measured; tumor necrosis factor-α (TNF-α) content in rats were detected by ELISA. Results Compared with the control group, the ALT activity and AST activity significantly increased, while SOD activity, GSH-Px activity, MDA content, and TNF-α content significantly decreased in the model group (till P<0.01). Compared with the model group, the ALT activity and AST activity significantly decreased, while SOD activity, GSH-Px activity, MDA content, and TNF-α content significantly increased in the low-dose ICA group and high-dose ICA group (all P<0.01). Conclusion ICA exerts the protective effect on the liver function of rats with acute liver injury induced by carbon tetrachloride through increasing the SOD activity and GSH-Px activity, decreasing MDA and TNF-α levels, thereby reducing free radical damage and inflammatory response. [ABSTRACT FROM AUTHOR]

目的探讨染色体微阵列分析(CMA)技术在脉络丛囊肿胎儿产前诊断中的应用价值。方法选择超声诊断为胎儿脉络丛囊肿孕妇50例,分别采用传统染色体G显带核型分析和CMA技术检测胎儿染色体异常情况。结果 50例超声确诊的胎儿脉络丛囊肿孕妇中,胎儿染色体正常45例、异常5例。其中,传统染色体G显带核型分析共检出胎儿染色体异常3例(18三体综合征2例、额外染色体1例),胎儿染色体异常率为6%; CMA技术共检出胎儿染色体异常5例(18三体综合征2例、额外染色体1例、染色体微重复2例),胎儿染色体异常率为10%。传统染色体G显带核型分析与CMA技术检出的2例18三体综合征一致,传统染色体G显带核型分析检出的1例额外染色体经CMA技术检测证实在15q11. 2-13. 1处有5. 45 Mb的微重复。另有2例经传统染色体G显带核型分析为正常核型,而经CMA技术检测证实1例在2p25. 3处有3. 85 Mb的微重复、1例在17p12处有1 346 kb的微重复,均为有明确致病性的片段重复。结论 CMA技术检测脉络丛囊肿胎儿染色体异常更加敏感,是传统染色体G显带核型分析的有益和必要补充。 [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

目的 观察长期瑞舒伐他汀灌胃对高脂模型大鼠学习记忆能力及海马显微结构的影响。方法 将85只学习记忆能力正常的SD大鼠随机分为正常对照组20只、模型组65只,模型组给予高脂饲料喂养,制作高脂血症模型。造模成功后随机分为模型对照组、高剂量组、常规剂量组,每组20只,分别经胃管给予生理盐水、瑞舒伐他汀4 mg/kg、瑞舒伐他汀0.5 mg/kg,正常对照组给予等量生理盐水,1次/d,连续5个月。观察各组大鼠学习记忆能力,HE染色观察各组大鼠海马结构的变化,Western blot方法检测海马Aβ1-42和磷酸化Tau蛋白的表达。结果与对照组比较,模型对照组逃避潜伏期明显延长,跨越次数明显减少;海马神经元细胞缺失、凋亡,神经元细胞结构不完整;海马组织中Aβ1-42和磷酸化Tau蛋白表达增加（P均<0.05）。高剂量组和常规剂量组逃避潜伏期较模型对照组缩短,跨越次数增加;海马锥体细胞层增厚,海马神经元排列整齐、紧密;海马组织中Aβ1-42和磷酸化Tau蛋白表达降低（P均<0.05）,与正常对照组比较差异均无统计学意义（P均>0.05）。结论 长期瑞舒伐他汀灌胃可提高高脂血症大鼠的学习记忆能力,其机制可能与降低海马Aβ1-42和磷酸化Tau蛋白表达,从而减少海马神经元凋亡、保护海马神经元结构的完整性有关。 [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

目的：探讨微环境个体化治疗对难治性烧伤残余创面细菌耐药的影响。方法选择难治性烧伤患者29例，随机分为观察组15例和对照组14例。两组均给予抗感染、营养支持等常规治疗，观察组按照药品说明书中关于药代动力学部分的描述，每日于血药浓度达到最高峰时立即进行残余创面换药；对照组则不限定换药时间。两组治疗2周评估创面，同时行细菌培养及药物敏感试验筛选耐药菌。结果两组治疗2周创面均缩小，缩小面积均大于原面积的20％，达到部分愈合标准，但均未完全愈合。观察组6例（40．0％）、对照组11例（78．6％）筛选出耐药菌，两组耐药菌筛出率比较P＜0．05。结论微环境个体化治疗有助于难治性烧伤患者残余创面的愈合，并能改善残余创面的细菌耐药情况。 [ABSTRACT FROM AUTHOR]

目的：观察辅助生殖技术促排卵周期中的无特异性病变宫腔积液（简称宫腔积液）对人高活力精子和小鼠胚胎发育潜能的影响。方法观察组将宫腔积液分别与精子培养液和胚胎培养液按体积浓度分别配制成25％、50％、75％、100％，对照组为单纯精子或胚胎培养液，在相应培养液中分别加入高活力人类精子和2细胞小鼠胚胎；观察接触宫腔积液后精子前向运动率和存活率，以及小鼠胚胎培养后囊胚形成率和孵出囊胚形成率。结果与对照组相比，观察组精子前向运动率、存活率及小鼠囊胚形成率、孵出率明显降低（P均＜0．05），且随宫腔积液浓度增加降低更明显（P均＜0．05）。结论宫腔积液可降低人高活力精子的运动和受精能力，同时降低胚胎发育潜能。 [ABSTRACT FROM AUTHOR]