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目的　分析自身免疫性葡萄膜炎（AU）患者外周血转录组差异表达基因。方法　选择AU患者4例，采集 外周静脉血 4 mL，分离有核细胞，采用流式细胞术检测 HLA-B27。利用高通量测序技术对细胞中所有 mRNA进行 测序，通过云平台分析获得与 AU 相关的差异表达基因、功能富集分析和蛋白-蛋白互作网络（PPI）等转录组学特 征。结果　样本有效浓度均达标，Q20、Q30符合测序质量要求，GC含量无明显偏倚，测序结果有效。共筛选出差异 表达基因2 638个，其中表达上调基因1 876个、表达下调基因762个；聚类分析显示，组内样本差异表达基因的高低 表达分布集中，组间样本则距离较远，提示差异表达基因的聚类性较好。差异表达基因 GO富集分析显示，差异表 达基因能够参与免疫应答、白细胞激活、细胞活化等生物学过程；KEGG通路富集分析显示，差异表达基因主要富集 于破骨细胞分化、NF-κB、趋化因子等信号通路；PPI分析发现，POLR2A、RBPJ、SUPT5H等基因处于PPI的核心位置。 结论 AU患者外周血转录组差异表达基因较多，其中 CXCL8、FFAR2、HBB等表达上调，SLC4A10、CD27、PAQR等 表达下调。这些差异表达基因能够通过多种途径参与AU的发生、发展。 [ABSTRACT FROM AUTHOR]

目的　探讨经翼点入路夹闭术对破裂胚胎型后交通动脉瘤破裂的疗效及影响患者预后的因素。方法　选 取经翼点入路夹闭术治疗的胚胎型后交通动脉瘤破裂患者119例，观察患者围术期并发症发生情况。术后6个月评 估神经功能恢复情况并进行治疗效果评价，采用格拉斯哥结局评分(GOS)将患者分为预后良好组(GOS评分 5分) 和预后不良组(GOS评分 1~4分)。比较预后良好和预后不良患者间的临床资料差异，采用多因素 Cox回归分析影 响患者预后的危险因素。结果　经翼点入路夹闭术治疗均成功，12例患者在围手术期出现并发症。术后6个月随 访，预后良好组89例，预后不良组30例，两组年龄、高血压史、Hunt-Hess分级和Fisher分级差异有统计学意义(P均< 0. 05)。多因素Cox回归分析显示高龄、有高血压史、Hunt-Hess分级和Fisher分级高是影响胚胎型后交通动脉瘤破 裂患者预后的独立危险因素(P均<0. 05)。结论　经翼点入路夹闭术治疗胚胎型后交通动脉瘤破裂安全有效，预后 不良与患者年龄、高血压病史以及Hunt-Hess分级、Fisher分级有关。 [ABSTRACT FROM AUTHOR]

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目的 探讨超声引导双侧腹横肌平面 (TAP）阻滞复合小剂量瑞芬太尼麻醉诱导在全麻剖宫产手术中的应 用效果. 方法　选取择期行全麻剖宫产术的产妇90例, 随机分为TAP阻滞组 (A组), TAP阻滞复合小剂量瑞芬太尼组 (B组), TAP阻滞复合中剂量瑞芬太尼组 (C组）各30例. 产妇均在麻醉诱导前30 min行超声引导下双侧腋中线水平 TAP阻滞, 麻醉诱导时B组推注瑞芬太尼0. 25 µg/kg, C组推注瑞芬太尼0. 5 µg/kg, A组推注等体积生理盐水. 分别 于麻醉诱导前 (T0 ), 切皮即刻 (T1 ), 胎儿取出即刻 (T2) 记录产妇收缩压 (SBP), 舒张压 (DBP), 平均动脉压 (MAP), 心率 (HR): 记录产妇术后24 h内镇痛泵按压次数及术后6, 12, 24 h的疼痛视觉模拟 (VAS）评分; 取胎儿脐动脉血进行血气 分析, 指标包括酸碱度 (pH), 血氧分压 (PO2 ), 二氧化碳分压 (PCO2 ), 乳酸 (Lac): 记录新生儿出生后1, 5 min Apgar评分, 记录需要触觉刺激, 面罩加压给氧, 气管插管的新生儿例数. 结果 与同组T0时点比较, B组, C组T1, T2时点SBP, DBP, MAP, HR均下降; 与同组T1时点比较, C组T2时点SBP, DBP, MAP, HR均下降; 与A组同时点比较, B组, C组T1, T2时点 SBP, DBP, MAP, HR均下降; 与B组同时点比较, C组T1, T2时点SBP, DBP, MAP, HR均下降 (P均<0. 05). 各组产妇术 后6, 12, 24 h VAS评分及24 h镇痛泵按压次数比较差异均无统计学意义, 各组新生儿脐动脉血气分析指标pH, PCO2, PO2, Lac比较差异均无统计学意义 (P均>0. 05). C组出生1 min Apgar评分<8分, 需要触觉刺激, 需要面罩通气的新生 儿例数高于A, B组 (P均<0. 05), A, B组各指标比较差异均无统计学意义. 结论　超声引导下双侧腹横肌平面阻滞技 术复合0. 25 µg/kg瑞芬太尼在全麻剖宫产手术中效果较好, 且应用安全. [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To investigate the effects and mechanisms of grape seed proanthocyanidin (GSP) on vulnerable plaques of apoE -I - mice. Methods Twenty-four apoE -I - mice were randomly divided into four groups: the model group and low-dose, medium-dose, high-dose GSP groups, which were then gavaged with normal saline and 45, 90, 180 mg/kg GSP, respectively, and meanwhile, they were fed with atherogenic fat diet for 8 weeks to induce the vulnerable plaques and at the end of experiment the descending aortas were dissected. HE, immunohistochemistry and special staining were used to detect the plaque areas and component macrophages and collagen included. Dihydroethidium (DHE) and TdT-mediated dUTP nick-end-labeling (TUNEL) staining were used for valuing oxidative stress and apoptosis; apoptosis-associated protein cytochrome C (CytC), Cleaved-Caspase-3 and Bax were tested by Western blotting. Results High-dose GSP suppressed aortic roots lesional areas, macrophage positive areas, and increased collagen content (all P < 0. 05), and decreased TUNEL as well as DHE-positive areas (all P < 0. Ol) ; the expression levels of pro-apoptotic protein Cytc, ClearedCaspase-3 and Bax in the high-dose GSP group were lower than those in the model group (all P < 0. 05 . 05). Conclusion GSP (180 mg/kg) stabå±±zed vulnerable lesions,æ³…ppressed lesional areas and reduced macrophages accumulation and cellular apoptosis by inhibiting oxidative stress and decreasing pro-apoptosis protein expression [ABSTRACT FROM AUTHOR]

Objective To investigate the effects of phosphatase SHP-2 on atherosclerotic plaque and macrophage（ Mφ） phenotype in Apo E knockout( Apo E-/-) mice. Methods Sixteen C57 mice were randomly divided into the following two groups: the model group and the experimental group, and mice in both groups were fed with high-fat diet for sixteen weeks. At the same time, the mice in the experimental group were intraperitoneally injected with 0. 5% PHPS1 3 mg/（ kg·d）, a SHP-2 inhibitor dissolved in 0. 5% DMSO, and the same amount of 0. 5% DMSO was injected daily into the mice of the model group. The plaque area of aorta and aortic root was assessed by using oil red O and Movat staining. The plaque macrophages（ galectin-3/MAC-2 positive area） and smooth muscle cell（ β-actin positive area） of aortic root were detected by immunohistochemical staining. The plaque collagen of aortic root was detected by Sirius red staining. The expression of type M1（ i NOS, TNF-α, and IL-6） and type M2（ IL-10, Arg-1, and FIZZ-1） macrophage marker genes in the aorta and aortic root was detected by real-time fluorescent quantitative polymerase chain reaction. Results The plaque area of the aorta and aortic root in the experimental group was smaller than that in the model group, with no statistically significant difference. Compared with the model group, the positive area of Mφ in the aortic root plaque of the experimental group decreased, and the positive area of smooth muscle cells and collagen increased（ P < 0. 05）. In the descending aortic plaques, the expression of M1-type Mφ gene markers decreased and the expression of M2 type Mφ gene markers increased（ both P < 0. 05）. Conclusion The phosphatase SHP-2 can inhibit the differentiation of Mφ to type M2 in plaque, which leads to the increase of plaque instability. [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To investigate the dose distribution characteristics of radioactive 125 I particle source in liver tissues and provide a reference for the brachytherapy dose calculations. Methods We applied the method of thermoluminescence dosimetry and used "M" glyph space to implant LiF (Mg, Cu, P). The relationships between the exposure dose (X) of the thermoluminescence dose element and the measured data (Y) were analyzed. Then, we measured and calculated the radiation absorbed dose changes and field distribution of 125 I radioactive particle sources in the liver tissues of the axial, radial and the four-point directions of the line. Results The reading of thermoluminescent dosimeter (Y) had linear relationship with the exposure dose (X). The linear regression equation was Y=0.134 2X-0.014 7 (R2 =0.999, P<0.01). With the increase of the distance from the source, the dose of 125I particle source in axial, radial and middle line showed a rapid decreasing tendency. According to different angles, the absorbed dose of equidistance locus were different, the radial dose >dose in middle line direction >the axial dose. Conclusion The dose distribution of 125 I particle source in liver tissues was uneven, and the dose distribution is similar to the"apple"shape with dose sag area and dose bump area distribution characteristics. [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)