144 results on '"Uematsu A"'
Search Results
2. Blood flow restriction reduces the increases in cardiorespiratory responses and subjective burden without inhibiting muscular activity during cycling at ventilatory threshold in healthy males
- Author
-
Azusa Uematsu, Yuta Mizushima, Hayato Ishizaka, Tibor Hortobágyi, Takashi Mizushima, Shigeru Toyoda, and Toshiaki Nakajima
- Subjects
Medicine ,Science - Published
- 2023
3. Relationship between institutional ventilated COVID-19 case volume and in-hospital death: A multicenter cohort study.
- Author
-
Shunsuke Amagasa, Satoko Uematsu, Mitsuru Kubota, Masahiro Kashiura, Hideto Yasuda, Mineji Hayakawa, Kazuma Yamakawa, Akira Endo, Takayuki Ogura, Atsushi Hirayama, Hideo Yasunaga, and Takashi Tagami
- Subjects
Medicine ,Science - Abstract
BackgroundThe volume-outcome relationship in patients with severe Coronavirus disease 2019 (COVID-19) is unclear and is important for establishing a system for the medical care of severe COVID-19. This study aimed to evaluate the association between institutional case volume and outcomes in patients with ventilated COVID-19.MethodsWe analyzed patients with severe COVID-19 on ventilatory control aged > 17 years who were enrolled in the J-RECOVER study, which is a retrospective multicenter observational study conducted between January 2020 and September 2020 in Japan. Based on the ventilated COVID-19 case volume, the higher one-third of institutions were defined as high-volume centers, the middle one-third as middle-volume centers, and the lower one-third as low-volume centers. The primary outcome measure was in-hospital mortality during hospitalization due to COVID-19. Multivariate logistic regression analysis for in-hospital mortality and ventilated COVID-19 case volume was performed after adjusting for multiple propensity scores and in-hospital variables. To estimate the multiple propensity score, we fitted a multinomial logistic regression model, which fell into one of the three groups based on patient demographics and prehospital factors.ResultsWe analyzed 561 patients who required ventilator management. In total, 159, 210, and 192 patients were admitted to low-volume (36 institutions, < 11 severe COVID-19 cases per institution during the study period), middle-volume (14 institutions, 11-25 severe cases per institution), and high-volume (5 institutions, > 25 severe cases per institution) centers, respectively. After adjustment for multiple propensity scores and in-hospital variables, admission to middle- and high-volume centers was not significantly associated with in-hospital death compared with admission to low-volume centers (adjusted odds ratio, 0.77 [95% confidence interval (CI): 0.46-1.29] and adjusted odds ratio, 0.76 [95% CI: 0.44-1.33], respectively).ConclusionsThere may be no significant relationship between institutional case volume and in-hospital mortality in patients with ventilated COVID-19.
- Published
- 2023
- Full Text
- View/download PDF
4. Blood flow restriction reduces the increases in cardiorespiratory responses and subjective burden without inhibiting muscular activity during cycling at ventilatory threshold in healthy males
- Author
-
Uematsu, Azusa, primary, Mizushima, Yuta, additional, Ishizaka, Hayato, additional, Hortobágyi, Tibor, additional, Mizushima, Takashi, additional, Toyoda, Shigeru, additional, and Nakajima, Toshiaki, additional
- Published
- 2023
- Full Text
- View/download PDF
5. Nintedanib induces gene expression changes in the lung of induced-rheumatoid arthritis-associated interstitial lung disease mice.
- Author
-
Shintaro Mikami, Yoko Miura, Shinji Kondo, Kosuke Sakai, Hiroaki Nishimura, Hiroyuki Kyoyama, Gaku Moriyama, Nobuyuki Koyama, Hideki Noguchi, Hirotsugu Ohkubo, Satoshi Kanazawa, and Kazutsugu Uematsu
- Subjects
Medicine ,Science - Abstract
Nintedanib is a multi-tyrosine kinase inhibitor widely used to treat progressive fibrosing interstitial lung diseases because it slows the reduction in forced vital capacity. However, the prognosis for patients treated with nintedanib remains poor. To improve nintedanib treatment, we examined the effects of nintedanib on gene expression in the lungs of induced-rheumatoid arthritis-associated interstitial lung disease model mice, which develop rheumatoid arthritis and subsequent pulmonary fibrosis. Using next-generation sequencing, we identified 27 upregulated and 130 downregulated genes in the lungs of these mice after treatment with nintedanib. The differentially expressed genes included mucin 5B and heat shock protein 70 family genes, which are related to interstitial lung diseases, as well as genes associated with extracellular components, particularly the myocardial architecture, suggesting unanticipated effects of nintedanib. Of the genes upregulated in the nintedanib-treated lung, expression of regulatory factor X2, which is suspected to be involved in cilia movement, and bone morphogenetic protein receptor type 2, which is involved in the pathology of pulmonary hypertension, was detected by immunohistochemistry and RNA in situ hybridization in peripheral airway epithelium and alveolar cells. Thus, the present findings indicate a set of genes whose expression alteration potentially underlies the effects of nintedanib on pulmonary fibrosis. It is expected that these findings will contribute to the development of improved nintedanib strategies for the treatment of progressive fibrosing interstitial lung diseases.
- Published
- 2022
- Full Text
- View/download PDF
6. Social and behavioral factors related to blood pressure measurement: A cross-sectional study in Bhutan.
- Author
-
Hiromi Kohori Segawa, Hironori Uematsu, Nidup Dorji, Ugyen Wangdi, Chencho Dorjee, Pemba Yangchen, Susumu Kunisawa, Ryota Sakamoto, and Yuichi Imanaka
- Subjects
Medicine ,Science - Abstract
Cardiovascular disease is a leading cause of death in the Kingdom of Bhutan, and early detection of hypertension is critical for preventing cardiovascular disease. However, health-seeking behavior, including blood pressure measurement, is infrequently investigated in Bhutan. Therefore, this study investigated factors related to blood pressure measurement in Bhutan. We performed a secondary data analysis of a target population of 1,962 individuals using data from the "2014 Bhutan STEPS survey data"as a cross-sectional study. Approximately 26% of those with hypertension who were detected during the STEPS survey had never had their blood pressure measured. Previous blood pressure measurement was significantly associated with age and working status in men (self-employed [odds ratio (OR): 0.219, 95% CI: 0.133-0.361], non-working [OR: 0.114, 95% CI: 0.050-0.263], employee [OR: 1.000]). Previous blood pressure measurement was significantly associated with higher income in women (Quartile-2 [OR: 1.984, 95% CI: 1.209-3.255], Quartile-1 [OR: 2.161, 95% CI: 1.415-3.299], Quartile-4 [OR: 1.000]). A family history of hypertension (OR: 2.019, 95% CI: 1.549-2.243) increased the likelihood of having experienced a blood pressure measurement in both men and women. Multivariate logistic regression showed that people with unhealthy lifestyles (high salt intake [adjusted odds ratio (AOR): 0.247, 95% confidence interval (CI): 0.068-0.893], tobacco use [AOR: 0.538, 95% CI: 0.380-0.761]) had a decreased likelihood of previous blood pressure measurement. To promote the early detection of hypertension in Bhutan, we suggest that more attention be paid to low-income women, non-working, self-employed, and low-income men, and a reduction of barriers to blood pressure measurement. Before the STEPS survey, a substantial number of hypertensive people had never had their blood pressure measured or were unconcerned about their health. As a result, we propose that early blood pressure monitoring and treatment for people with hypertension or at higher risk of hypertension be given increased emphasis.
- Published
- 2022
- Full Text
- View/download PDF
7. Carotid artery intima-media thickness, HDL cholesterol levels, and gender associated with poor visual acuity in patients with branch retinal artery occlusion.
- Author
-
Masayuki Yasuda, Hajime Sato, Kazuki Hashimoto, Urara Osada, Takehiro Hariya, Hiroko Nakayama, Toshifumi Asano, Noriyuki Suzuki, Tatsu Okabe, Mai Yamazaki, Megumi Uematsu, Masanori Munakata, and Toru Nakazawa
- Subjects
Medicine ,Science - Abstract
PurposeTo investigate factors associated with poor visual acuity (VA) in branch retinal artery occlusion (BRAO).MethodsThis was a retrospective cross-sectional study of 72 eyes with BRAO of 72 patients. For statistical comparison, we divided the patients into worse-VA (decimal VA < 0.5) and better-VA (decimal VA > = 0.5) groups. We examined the association of clinical findings, including blood biochemical test data and carotid artery ultrasound parameters, with poor VA.ResultsMedian age, hematocrit, hemoglobin and high-density lipoprotein (HDL) differed significantly between the groups (P = 0.018, P < 0.01, P < 0.01, and P = 0.025). There was a tendency towards higher median IMT-Bmax in the worse-VA group (worse-VA vs. better-VA: 2.70 mm vs. 1.60 mm, P = 0.152). Spearman's rank correlation test revealed that logMAR VA was significantly correlated to IMT-Bmax (rs = 0.31, P < 0.01) and IMT-Cmax (rs = 0.24, P = 0.035). Furthermore, logMAR VA was significantly correlated to HDL level (rs = -0.33, P < 0.01). Multivariate logistic regression analysis revealed that IMT-Bmax (odds ratio [OR] = 2.70, P = 0.049), HDL level (OR = 0.91, P = 0.032), and female gender (OR = 15.63, P = 0.032) were independently associated with worse VA in BRAO.ConclusionsWe found that increased IMT-Bmax, decreased HDL, and female sex were associated with poor VA in BRAO patients. Our findings might suggest novel risk factors for visual dysfunction in BRAO and may provide new insights into the pathomechanisms underlying BRAO.
- Published
- 2020
- Full Text
- View/download PDF
8. Synergistic effect of collagen and CXCL12 in the low doses on human platelet activation.
- Author
-
Daiki Nakashima, Takashi Onuma, Kumiko Tanabe, Yuko Kito, Kodai Uematsu, Daisuke Mizutani, Yukiko Enomoto, Masanori Tsujimoto, Tomoaki Doi, Rie Matsushima-Nishiwaki, Haruhiko Tokuda, Shinji Ogura, Toru Iwama, Osamu Kozawa, and Hiroki Iida
- Subjects
Medicine ,Science - Abstract
CXCL12, also known as stromal cell-derived factor-1, is a chemokine classified into CXC families, which exerts its function by binding to specific receptors called CXCR4 and CXCR7. Human platelets express CXCR4 and CXCR7 on the plasma membrane. It has been reported that CXCL12 potentiates to induce platelet aggregation in cooperation with agonists including collagen. However, the precise roles and mechanisms of CXCL12 in human platelet activation are not fully elucidated. In the present study, we investigated the effect of simultaneous stimulation with low doses of collagen and CXCL12 on the activation of human platelets. The simultaneous stimulation with collagen and CXCL12 induced the secretion of platelet-derived growth factor (PDGF)-AB and the release of soluble CD40 ligand (sCD40L) from human platelets in addition to their aggregation, despite the fact that the simultaneous stimulation with thrombin receptor-activating peptide (TRAP) or adenosine diphosphate (ADP), and CXCL12 had little effects on the platelet aggregation. The agonist of Glycoprotein (GP) Ⅵ convulxin and CXCL12 also induced platelet aggregation synergistically. The monoclonal antibody against CXCR4 but not CXCR7 suppressed the platelet aggregation induced by simultaneous stimulation with collagen and CXCL12. The phosphorylation of p38 mitogen-activated protein kinase (MAPK), but not p44/p42 MAPK, was induced by the simultaneous stimulation. In addition, the simultaneous stimulation with collagen and CXCL12 induced the phosphorylation of HSP27 and the subsequent release of phosphorylated-HSP27 from human platelets. SB203580, a specific inhibitor of p38 MAPK, attenuated the platelet aggregation, the phosphorylation of p38 MAPK and HSP27, the PDGF-AB secretion, the sCD40L release and the phosphorylated-HSP27 release induced by the simultaneous stimulation with collagen and CXCL12. These results strongly suggest that collagen and CXCL12 in low doses synergistically act to induce PDGF-AB secretion, sCD40L release and phosphorylated-HSP27 release from activated human platelets via p38 MAPK activation.
- Published
- 2020
- Full Text
- View/download PDF
9. Daily calving frequency and preterm calving is not associated with lunar cycle but preterm calving is associated with weather conditions in Japanese Black cows.
- Author
-
Yosuke Sasaki, Narumi Kitai, Mizuho Uematsu, Go Kitahara, and Takeshi Osawa
- Subjects
Medicine ,Science - Abstract
Several external factors including lunar cycle and weather conditions might be associated with calving conditions. Our objective here was to determine the effects of lunar cycle and weather conditions on calving frequency and the occurrence of preterm calving in Japanese Black cows. Calving records were obtained from 905 farms in Miyazaki Prefecture, Japan. Data were collected from 41,116 calvings. We conducted two studies: Study 1 investigated the effects of lunar cycle and weather conditions on daily calving frequency (DCF) with the observational unit of each day and Study 2 investigated those effects on the occurrence of preterm calving with the observational unit for each calving. Preterm calving was defined by whether or not a cow calving before 280 days of gestation, lower 10th percentile of gestation length of the collected data, and by whether or not a cow calving before 289 days of gestation, median of the gestation length. For Study 1, lunar cycle was not associated with DCF in all cows, in only primiparous cows and in only multiparous cows. As well as lunar cycle, weather conditions such as temperature, diurnal temperature variation, the temperature-humidity index, precipitation amount, barometric pressure, relative humidity and solar radiation, were also not associated with DCF. For Study 2, lunar cycle phases were not associated with the occurrence of preterm calving. However, preterm calving was associated with all of the weather conditions (P < 0.05) except for precipitation amounts and solar radiation. Temperature, the temperature-humidity index and relative humidity were positively associated with the occurrence of preterm calving. In contrast, diurnal temperature variations and barometric pressure were negatively associated with the occurrence of preterm calving. In conclusion, the lunar cycle was not associated with DCF and preterm calving, but the weather conditions were associated with preterm calving.
- Published
- 2019
- Full Text
- View/download PDF
10. Social and behavioral factors related to blood pressure measurement: A cross-sectional study in Bhutan
- Author
-
Kohori Segawa, Hiromi, primary, Uematsu, Hironori, additional, Dorji, Nidup, additional, Wangdi, Ugyen, additional, Dorjee, Chencho, additional, Yangchen, Pemba, additional, Kunisawa, Susumu, additional, Sakamoto, Ryota, additional, and Imanaka, Yuichi, additional
- Published
- 2022
- Full Text
- View/download PDF
11. Relationship between institutional ventilated COVID-19 case volume and in-hospital death: A multicenter cohort study.
- Author
-
Amagasa, Shunsuke, Uematsu, Satoko, Kubota, Mitsuru, Kashiura, Masahiro, Yasuda, Hideto, Hayakawa, Mineji, Yamakawa, Kazuma, Endo, Akira, Ogura, Takayuki, Hirayama, Atsushi, Yasunaga, Hideo, and Tagami, Takashi
- Subjects
- *
COVID-19 pandemic , *PROPENSITY score matching , *COVID-19 , *HOSPITAL statistics , *LOGISTIC regression analysis , *COHORT analysis , *HOSPITAL mortality - Abstract
Background: The volume-outcome relationship in patients with severe Coronavirus disease 2019 (COVID-19) is unclear and is important for establishing a system for the medical care of severe COVID-19. This study aimed to evaluate the association between institutional case volume and outcomes in patients with ventilated COVID-19. Methods: We analyzed patients with severe COVID-19 on ventilatory control aged > 17 years who were enrolled in the J-RECOVER study, which is a retrospective multicenter observational study conducted between January 2020 and September 2020 in Japan. Based on the ventilated COVID-19 case volume, the higher one-third of institutions were defined as high-volume centers, the middle one-third as middle-volume centers, and the lower one-third as low-volume centers. The primary outcome measure was in-hospital mortality during hospitalization due to COVID-19. Multivariate logistic regression analysis for in-hospital mortality and ventilated COVID-19 case volume was performed after adjusting for multiple propensity scores and in-hospital variables. To estimate the multiple propensity score, we fitted a multinomial logistic regression model, which fell into one of the three groups based on patient demographics and prehospital factors. Results: We analyzed 561 patients who required ventilator management. In total, 159, 210, and 192 patients were admitted to low-volume (36 institutions, < 11 severe COVID-19 cases per institution during the study period), middle-volume (14 institutions, 11–25 severe cases per institution), and high-volume (5 institutions, > 25 severe cases per institution) centers, respectively. After adjustment for multiple propensity scores and in-hospital variables, admission to middle- and high-volume centers was not significantly associated with in-hospital death compared with admission to low-volume centers (adjusted odds ratio, 0.77 [95% confidence interval (CI): 0.46–1.29] and adjusted odds ratio, 0.76 [95% CI: 0.44–1.33], respectively). Conclusions: There may be no significant relationship between institutional case volume and in-hospital mortality in patients with ventilated COVID-19. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
12. Nintedanib induces gene expression changes in the lung of induced-rheumatoid arthritis–associated interstitial lung disease mice
- Author
-
Mikami, Shintaro, primary, Miura, Yoko, additional, Kondo, Shinji, additional, Sakai, Kosuke, additional, Nishimura, Hiroaki, additional, Kyoyama, Hiroyuki, additional, Moriyama, Gaku, additional, Koyama, Nobuyuki, additional, Noguchi, Hideki, additional, Ohkubo, Hirotsugu, additional, Kanazawa, Satoshi, additional, and Uematsu, Kazutsugu, additional
- Published
- 2022
- Full Text
- View/download PDF
13. The Impact of Patient Profiles and Procedures on Hospitalization Costs through Length of Stay in Community-Acquired Pneumonia Patients Based on a Japanese Administrative Database.
- Author
-
Hironori Uematsu, Susumu Kunisawa, Kazuto Yamashita, and Yuichi Imanaka
- Subjects
Medicine ,Science - Abstract
BackgroundCommunity-acquired pneumonia is a common cause of patient hospitalization, and its burden on health care systems is increasing in aging societies. In this study, we aimed to investigate the factors that affect hospitalization costs in community-acquired pneumonia patients while considering the intermediate influence of patient length of stay.MethodsUsing a multi-institutional administrative claims database, we analyzed 30,041 patients hospitalized for community-acquired pneumonia who had been discharged between April 1, 2012 and September 30, 2013 from 289 acute care hospitals in Japan. Possible factors associated with hospitalization costs were investigated using structural equation modeling with length of stay as an intermediate variable. We calculated the direct, indirect (through length of stay), and total effects of the candidate factors on hospitalization costs in the model. Lastly, we calculated the ratio of indirect effects to direct effects for each factor.ResultsThe structural equation model showed that higher disease severities (using A-DROP, Barthel Index, and Charlson Comorbidity Index scores), use of mechanical ventilation, and tube feeding were associated with higher hospitalization costs, regardless of the intermediate influence of length of stay. The severity factors were also associated with longer length of stay durations. The ratio of indirect effects to direct effects on total hospitalization costs showed that the former was greater than the latter in the factors, except in the use of mechanical ventilation.ConclusionsOur structural equation modeling analysis indicated that patient profiles and procedures impacted on hospitalization costs both directly and indirectly. Furthermore, the profiles were generally shown to have greater indirect effects (through length of stay) on hospitalization costs than direct effects. These findings may be useful in supporting the more appropriate distribution of health care resources.
- Published
- 2015
- Full Text
- View/download PDF
14. Expression and Characterization of Recombinant, Tetrameric and Enzymatically Active Influenza Neuraminidase for the Setup of an Enzyme-Linked Lectin-Based Assay.
- Author
-
Marua Prevato, Ilaria Ferlenghi, Alessandra Bonci, Yasushi Uematsu, Giulia Anselmi, Fabiola Giusti, Sylvie Bertholet, Francois Legay, John Laird Telford, Ethan C Settembre, Domenico Maione, and Roberta Cozzi
- Subjects
Medicine ,Science - Abstract
Developing a universal influenza vaccine that induces broad spectrum and longer-term immunity has become an important potentially achievable target in influenza vaccine research and development. Hemagglutinin (HA) and neuraminidase (NA) are the two major influenza virus antigens. Although antibody responses against influenza virus are mainly directed toward HA, NA is reported to be more genetically stable; hence NA-based vaccines have the potential to be effective for longer time periods. NA-specific immunity has been shown to limit the spread of influenza virus, thus reducing disease symptoms and providing cross-protection against heterosubtypic viruses in mouse challenge experiments. The production of large quantities of highly pure and stable NA could be beneficial for the development of new antivirals, subunit-based vaccines, and novel diagnostic tools. In this study, recombinant NA (rNA) was produced in mammalian cells at high levels from both swine A/California/07/2009 (H1N1) and avian A/turkey/Turkey/01/2005 (H5N1) influenza viruses. Biochemical, structural, and immunological characterizations revealed that the soluble rNAs produced are tetrameric, enzymatically active and immunogenic, and finally they represent good alternatives to conventionally used sources of NA in the Enzyme-Linked Lectin Assay (ELLA).
- Published
- 2015
- Full Text
- View/download PDF
15. Effect of advanced periodontal self-care in patients with early-stage periodontal diseases on endothelial function: An open-label, randomized controlled trial
- Author
-
Okada, Ayako, primary, Murata, Takatoshi, additional, Matin, Khairul, additional, Ariyoshi, Meu, additional, Otsuka, Ryoko, additional, Yamashita, Mamiko, additional, Suzuki, Masayuki, additional, Wakiyama, Rumi, additional, Tateno, Ken, additional, Suzuki, Megumi, additional, Aoyagi, Hitomi, additional, Uematsu, Hiromi, additional, Imamura, Akiko, additional, Kosaka, Miki, additional, Mizukaki, Tomoko, additional, Sato, Tsutomu, additional, Kawahara, Hiroshi, additional, and Hanada, Nobuhiro, additional
- Published
- 2021
- Full Text
- View/download PDF
16. Gender with marital status, cultural differences, and vulnerability to hypertension: Findings from the national survey for noncommunicable disease risk factors and mental health using WHO STEPS in Bhutan
- Author
-
Segawa, Hiromi Kohori, primary, Uematsu, Hironori, additional, Dorji, Nidup, additional, Wangdi, Ugyen, additional, Dorjee, Chencho, additional, Yangchen, Pemba, additional, Kunisawa, Susumu, additional, Sakamoto, Ryota, additional, and Imanaka, Yuichi, additional
- Published
- 2021
- Full Text
- View/download PDF
17. Amyloid β-mediated Zn2+ influx into dentate granule cells transiently induces a short-term cognitive deficit.
- Author
-
Atsushi Takeda, Masatoshi Nakamura, Hiroaki Fujii, Chihiro Uematsu, Tatsuya Minamino, Paul A Adlard, Ashley I Bush, and Haruna Tamano
- Subjects
Medicine ,Science - Abstract
We examined an idea that short-term cognition is transiently affected by a state of confusion in Zn2+ transport system due to a local increase in amyloid-β (Aβ) concentration. A single injection of Aβ (25 pmol) into the dentate gyrus affected dentate gyrus long-term potentiation (LTP) 1 h after the injection, but not 4 h after the injection. Simultaneously, 1-h memory of object recognition was affected when the training was performed 1 h after the injection, but not 4 h after the injection. Aβ-mediated impairments of LTP and memory were rescued in the presence of zinc chelators, suggesting that Zn2+ is involved in Aβ action. When Aβ was injected into the dentate gyrus, intracellular Zn2+ levels were increased only in the injected area in the dentate gyrus, suggesting that Aβ induces the influx of Zn2+ into cells in the injected area. When Aβ was added to hippocampal slices, Aβ did not increase intracellular Zn2+ levels in the dentate granule cell layer in ACSF without Zn2+, but in ACSF containing Zn2+. The increase in intracellular Zn2+ levels was inhibited in the presence of CaEDTA, an extracellular zinc chelator, but not in the presence of CNQX, an AMPA receptor antagonist. The present study indicates that Aβ-mediated Zn2+ influx into dentate granule cells, which may occur without AMPA receptor activation, transiently induces a short-term cognitive deficit. Extracellular Zn2+ may play a key role for transiently Aβ-induced cognition deficits.
- Published
- 2014
- Full Text
- View/download PDF
18. Prosaposin overexpression following kainic acid-induced neurotoxicity.
- Author
-
Hiroaki Nabeka, Keigo Uematsu, Hiroko Takechi, Tetsuya Shimokawa, Kimiko Yamamiya, Cheng Li, Takuya Doihara, Shouichiro Saito, Naoto Kobayashi, and Seiji Matsuda
- Subjects
Medicine ,Science - Abstract
Because excessive glutamate release is believed to play a pivotal role in numerous neuropathological disorders, such as ischemia or seizure, we aimed to investigate whether intrinsic prosaposin (PS), a neuroprotective factor when supplied exogenously in vivo or in vitro, is up-regulated after the excitotoxicity induced by kainic acid (KA), a glutamate analog. In the present study, PS immunoreactivity and its mRNA expression in the hippocampal and cortical neurons showed significant increases on day 3 after KA injection, and high PS levels were maintained even after 3 weeks. The increase in PS, but not saposins, detected by immunoblot analysis suggests that the increase in PS-like immunoreactivity after KA injection was not due to an increase in saposins as lysosomal enzymes after neuronal damage, but rather to an increase in PS as a neurotrophic factor to improve neuronal survival. Furthermore, several neurons with slender nuclei inside/outside of the pyramidal layer showed more intense PS mRNA expression than other pyramidal neurons. Based on the results from double immunostaining using anti-PS and anti-GABA antibodies, these neurons were shown to be GABAergic interneurons in the extra- and intra-pyramidal layers. In the cerebral cortex, several large neurons in the V layer showed very intense PS mRNA expression 3 days after KA injection. The choroid plexus showed intense PS mRNA expression even in the normal rat, and the intensity increased significantly after KA injection. The present study indicates that inhibitory interneurons as well as stimulated hippocampal pyramidal and cortical neurons synthesize PS for neuronal survival, and the choroid plexus is highly activated to synthesize PS, which may prevent neurons from excitotoxic neuronal damage. To the best of our knowledge, this is the first study that demonstrates axonal transport and increased production of neurotrophic factor PS after KA injection.
- Published
- 2014
- Full Text
- View/download PDF
19. Gender with marital status, cultural differences, and vulnerability to hypertension: Findings from the national survey for noncommunicable disease risk factors and mental health using WHO STEPS in Bhutan
- Author
-
Ryota Sakamoto, Susumu Kunisawa, Nidup Dorji, Chencho Dorjee, Ugyen Wangdi, Pemba Yangchen, Hironori Uematsu, Yuichi Imanaka, and Hiromi Segawa
- Subjects
Male ,Culture ,Social Sciences ,Disease ,Cardiovascular Medicine ,Logistic regression ,Vascular Medicine ,Geographical Locations ,Medical Conditions ,Divorce ,Risk Factors ,Noncommunicable diseases ,Medicine and Health Sciences ,Medicine ,Psychology ,Mental health and psychiatry ,Bhutan ,Cause of death ,Language ,Multidisciplinary ,Single Person ,Middle Aged ,Cardiovascular diseases ,Hypertension ,Blood pressure ,Marital status ,Research Article ,Adult ,Asia ,Adolescent ,Science ,Cardiology ,Odds ,Young Adult ,Sex Factors ,Humans ,Aged ,Marital Status ,business.industry ,Cognitive Psychology ,Biology and Life Sciences ,Secondary data ,Odds ratio ,Widowhood ,Cardiovascular disease risk ,Mental health ,Health Surveys ,Cross-Sectional Studies ,People and Places ,Cognitive Science ,business ,Demography ,Neuroscience - Abstract
Cardiovascular disease is the leading cause of death in the Kingdom of Bhutan. Thus, the early detection and prevention of hypertension is critical for reducing cardiovascular disease. However, the influence of sociocultural factors on vulnerability to hypertension needs further investigation. This study performed secondary data analysis on 1,909 individuals in a cross-sectional study (the National survey for noncommunicable disease risk factors and mental health using World Health Organization (WHO) STEPS approach in Bhutan– 2014). Multivariate logistic regression demonstrated a significant association between gender with marital status and hypertension. Women had a higher odds ratio than men (Ref) when married (AOR: 1.27, 95% confidence intervals (CI): 1.23–1.31), and when separated, divorced, or widowed (AOR: 1.18, 95% CI: 1.12–1.26). People who speak the Tshanglakha language scored the highest odds (AOR: 1.24, 95% CI: 1.20–1.27), followed by Lhotshamkha (AOR: 1.09, 95% CI: 1.06–1.12) and Dzongkha (Ref) after adjusting for various social and biomedical factors. Additionally, tobacco use displayed decreased odds for hypertension. To promote the early detection and prevention of hypertension, these cultural factors should be considered even within small geographic areas, such as Bhutan. It is necessary to strengthen hypertension preventive strategies for people who speak Tshanglakha and Lhotshamkha. Furthermore, careful consideration should be given to preventing hypertension among adults aged 40 years or more, women who are married, separated, divorced, or widowed, and men who never married in Bhutan.
- Published
- 2021
20. Effect of advanced periodontal self-care in patients with early-stage periodontal diseases on endothelial function: An open-label, randomized controlled trial
- Author
-
Hiroshi Kawahara, Tomoko Mizukaki, Megumi Suzuki, Nobuhiro Hanada, Mamiko Yamashita, Rumi Wakiyama, Ken Tateno, Akiko Imamura, Meu Ariyoshi, Tsutomu Sato, Ayako Okada, Takatoshi Murata, Hiromi Uematsu, Ryoko Otsuka, Masayuki Suzuki, Miki Kosaka, Hitomi Aoyagi, and Khairul Matin
- Subjects
Male ,Teeth ,Brachial Artery ,Mandible ,Vascular Medicine ,Gastroenterology ,law.invention ,Geographical Locations ,chemistry.chemical_compound ,Medical Conditions ,Oral Diseases ,Randomized controlled trial ,Japan ,law ,Medicine and Health Sciences ,Maxilla ,Stage (cooking) ,Brachial artery ,Multidisciplinary ,Arteries ,Middle Aged ,Treatment Outcome ,Medicine ,Female ,Anatomy ,medicine.symptom ,Research Article ,Dilatation, Pathologic ,Adult ,medicine.medical_specialty ,Drug Research and Development ,Asia ,Patients ,Science ,Oral Medicine ,Bleeding on probing ,Hemorrhage ,Research and Analysis Methods ,Arginine ,Young Adult ,Signs and Symptoms ,medicine.artery ,Internal medicine ,medicine ,Humans ,Clinical Trials ,In patient ,Periodontal Diseases ,Aged ,Pharmacology ,business.industry ,Biology and Life Sciences ,Atherosclerosis ,Oral Hygiene ,Randomized Controlled Trials ,Confidence interval ,Health Care ,Clinical trial ,Self Care ,Jaw ,chemistry ,People and Places ,Cardiovascular Anatomy ,Blood Vessels ,Endothelium, Vascular ,Clinical Medicine ,Asymmetric dimethylarginine ,business ,Digestive System ,Head ,Biomarkers ,Follow-Up Studies - Abstract
Although a significant association between periodontal disease and atherosclerotic cardiovascular disease has been reported, their cause-to-effect relationship remains controversial. This randomized controlled clinical trial aimed to investigate the effect of advanced self-care on atherosclerotic cardiovascular disease-related vascular function markers flow-mediated brachial artery dilatation (FMD) and serum asymmetric dimethylarginine (ADMA) level in patients with early-stage periodontal disease. The study was designed as a parallel group, 3-month follow-up, open-label, randomized controlled trial. The control group received standard care for periodontal diseases, whereas the test group additionally applied disinfectant using a custom-fabricated prescription tray for advanced self-care twice a day. Overall, 110 patients provided data for FMD and serum ADMA level. No significant improvements in FMD were observed in the control (mean increase, −0.1%; 95% confidence interval [CI], −1.0–0.8; P = 0.805) or test (mean increase, −0.3%; 95% CI, −1.1–0.4; P = 0.398) group. No significant changes in serum ADMA levels were observed (mean reduction, 0.01 μmol/L; 95% CI, −0.00–0.02; P = 0.366 and mean reduction, 0.00 μmol/L; 95% CI, −0.01–0.01; P = 0.349, respectively). No significant between-group differences were found in FMD (mean difference, −0.2%; 95% CI, −1.4–0.9; p = 0.708) or serum ADMA levels (mean difference, 0.01 nmol/L; 95% CI, −0.00–0.03; p = 0.122). Significant improvements in the average probing pocket depth were observed in the control and test groups. The bleeding on probing score in the test group was significantly reduced, while that in the control group was reduced, although not significantly. Periodontal care for a 3-month duration did not provide better endothelial function although improvements of periodontal status in patients with early-stage periodontal diseases. This trial is registered in UMIN Clinical Trials Registry (www.umin.ac.jp/ctr/; ID: UMIN000023395).
- Published
- 2021
21. Synergistic effect of collagen and CXCL12 in the low doses on human platelet activation
- Author
-
Nakashima, Daiki, primary, Onuma, Takashi, additional, Tanabe, Kumiko, additional, Kito, Yuko, additional, Uematsu, Kodai, additional, Mizutani, Daisuke, additional, Enomoto, Yukiko, additional, Tsujimoto, Masanori, additional, Doi, Tomoaki, additional, Matsushima-Nishiwaki, Rie, additional, Tokuda, Haruhiko, additional, Ogura, Shinji, additional, Iwama, Toru, additional, Kozawa, Osamu, additional, and Iida, Hiroki, additional
- Published
- 2020
- Full Text
- View/download PDF
22. Carotid artery intima-media thickness, HDL cholesterol levels, and gender associated with poor visual acuity in patients with branch retinal artery occlusion
- Author
-
Yasuda, Masayuki, primary, Sato, Hajime, additional, Hashimoto, Kazuki, additional, Osada, Urara, additional, Hariya, Takehiro, additional, Nakayama, Hiroko, additional, Asano, Toshifumi, additional, Suzuki, Noriyuki, additional, Okabe, Tatsu, additional, Yamazaki, Mai, additional, Uematsu, Megumi, additional, Munakata, Masanori, additional, and Nakazawa, Toru, additional
- Published
- 2020
- Full Text
- View/download PDF
23. Daily calving frequency and preterm calving is not associated with lunar cycle but preterm calving is associated with weather conditions in Japanese Black cows
- Author
-
Narumi Kitai, Yosuke Sasaki, Takeshi Osawa, Go Kitahara, and Mizuho Uematsu
- Subjects
Atmospheric Science ,Maternal Health ,Ice calving ,0403 veterinary science ,Lunar Cycle ,Labor and Delivery ,Pregnancy ,Animal Products ,Medicine and Health Sciences ,Moon ,Mammals ,Multidisciplinary ,Physics ,Electromagnetic Radiation ,food and beverages ,Obstetrics and Gynecology ,Eukaryota ,Agriculture ,04 agricultural and veterinary sciences ,Ruminants ,Physical Sciences ,Vertebrates ,Medicine ,Gestation ,Solar Radiation ,Female ,Gestation length ,Beef ,Research Article ,Meat ,040301 veterinary sciences ,Science ,Gestation period ,Biology ,Preterm Birth ,Animal science ,Meteorology ,Negatively associated ,Bovines ,medicine ,Animals ,Weather ,Nutrition ,Diurnal temperature variation ,0402 animal and dairy science ,Organisms ,Biology and Life Sciences ,Humidity ,medicine.disease ,040201 dairy & animal science ,Diet ,Pregnancy Complications ,Food ,Amniotes ,Earth Sciences ,Birth ,Women's Health ,Pregnancy, Animal ,Cattle - Abstract
Several external factors including lunar cycle and weather conditions might be associated with calving conditions. Our objective here was to determine the effects of lunar cycle and weather conditions on calving frequency and the occurrence of preterm calving in Japanese Black cows. Calving records were obtained from 905 farms in Miyazaki Prefecture, Japan. Data were collected from 41,116 calvings. We conducted two studies: Study 1 investigated the effects of lunar cycle and weather conditions on daily calving frequency (DCF) with the observational unit of each day and Study 2 investigated those effects on the occurrence of preterm calving with the observational unit for each calving. Preterm calving was defined by whether or not a cow calving before 280 days of gestation, lower 10th percentile of gestation length of the collected data, and by whether or not a cow calving before 289 days of gestation, median of the gestation length. For Study 1, lunar cycle was not associated with DCF in all cows, in only primiparous cows and in only multiparous cows. As well as lunar cycle, weather conditions such as temperature, diurnal temperature variation, the temperature-humidity index, precipitation amount, barometric pressure, relative humidity and solar radiation, were also not associated with DCF. For Study 2, lunar cycle phases were not associated with the occurrence of preterm calving. However, preterm calving was associated with all of the weather conditions (P < 0.05) except for precipitation amounts and solar radiation. Temperature, the temperature-humidity index and relative humidity were positively associated with the occurrence of preterm calving. In contrast, diurnal temperature variations and barometric pressure were negatively associated with the occurrence of preterm calving. In conclusion, the lunar cycle was not associated with DCF and preterm calving, but the weather conditions were associated with preterm calving.
- Published
- 2019
24. Carotid artery intima-media thickness, HDL cholesterol levels, and gender associated with poor visual acuity in patients with branch retinal artery occlusion
- Author
-
Tatsu Okabe, Toshifumi Asano, Takehiro Hariya, Urara Osada, Megumi Uematsu, Masanori Munakata, Hiroko Nakayama, Mai Yamazaki, Toru Nakazawa, Noriyuki Suzuki, Hajime Sato, Masayuki Yasuda, and Kazuki Hashimoto
- Subjects
Male ,Visual acuity ,Retinal Artery Occlusion ,Vision ,Visual Acuity ,Social Sciences ,Cardiovascular Medicine ,030204 cardiovascular system & hematology ,Hematocrit ,Eye ,Carotid Intima-Media Thickness ,Vascular Medicine ,Biochemistry ,chemistry.chemical_compound ,Medical Conditions ,0302 clinical medicine ,Medicine and Health Sciences ,Psychology ,Multidisciplinary ,medicine.diagnostic_test ,Arteries ,Middle Aged ,C-Reactive Proteins ,Carotid Arteries ,Cardiovascular Diseases ,Cardiology ,Medicine ,Female ,Sensory Perception ,Anatomy ,medicine.symptom ,Research Article ,medicine.medical_specialty ,Lipoproteins ,Science ,Vision Disorders ,03 medical and health sciences ,Branch retinal artery occlusion ,Ocular System ,Internal medicine ,medicine ,Humans ,Aged ,Retrospective Studies ,Rank correlation ,business.industry ,Cholesterol ,Cholesterol, HDL ,Cognitive Psychology ,Biology and Life Sciences ,Proteins ,Odds ratio ,Cardiovascular Disease Risk ,Atherosclerosis ,medicine.disease ,Cross-Sectional Studies ,chemistry ,Intima-media thickness ,Cardiovascular Anatomy ,Blood Vessels ,Cognitive Science ,Eyes ,Perception ,business ,Head ,030217 neurology & neurosurgery ,Neuroscience ,Lipoprotein - Abstract
PurposeTo investigate factors associated with poor visual acuity (VA) in branch retinal artery occlusion (BRAO).MethodsThis was a retrospective cross-sectional study of 72 eyes with BRAO of 72 patients. For statistical comparison, we divided the patients into worse-VA (decimal VA < 0.5) and better-VA (decimal VA > = 0.5) groups. We examined the association of clinical findings, including blood biochemical test data and carotid artery ultrasound parameters, with poor VA.ResultsMedian age, hematocrit, hemoglobin and high-density lipoprotein (HDL) differed significantly between the groups (P = 0.018, P < 0.01, P < 0.01, and P = 0.025). There was a tendency towards higher median IMT-Bmax in the worse-VA group (worse-VA vs. better-VA: 2.70 mm vs. 1.60 mm, P = 0.152). Spearman's rank correlation test revealed that logMAR VA was significantly correlated to IMT-Bmax (rs = 0.31, P < 0.01) and IMT-Cmax (rs = 0.24, P = 0.035). Furthermore, logMAR VA was significantly correlated to HDL level (rs = -0.33, P < 0.01). Multivariate logistic regression analysis revealed that IMT-Bmax (odds ratio [OR] = 2.70, P = 0.049), HDL level (OR = 0.91, P = 0.032), and female gender (OR = 15.63, P = 0.032) were independently associated with worse VA in BRAO.ConclusionsWe found that increased IMT-Bmax, decreased HDL, and female sex were associated with poor VA in BRAO patients. Our findings might suggest novel risk factors for visual dysfunction in BRAO and may provide new insights into the pathomechanisms underlying BRAO.
- Published
- 2020
25. Daily calving frequency and preterm calving is not associated with lunar cycle but preterm calving is associated with weather conditions in Japanese Black cows
- Author
-
Sasaki, Yosuke, primary, Kitai, Narumi, additional, Uematsu, Mizuho, additional, Kitahara, Go, additional, and Osawa, Takeshi, additional
- Published
- 2019
- Full Text
- View/download PDF
26. Targeted DNA methylation in pericentromeres with genome editing-based artificial DNA methyltransferase
- Author
-
Yamazaki, T., Hatano, Y., Handa, T., Kato, S., Hoida, K., Yamamura, R., Fukuyama, T., Uematsu, T., Kobayashi, N., Kimura, Hiroshi, and Yamagata, K.
- Subjects
0301 basic medicine ,Embryology ,Bisulfite sequencing ,Clustered Regularly Interspaced Short Palindromic Repeats/genetics ,lcsh:Medicine ,DNA Methylation/*genetics ,Gene Editing/*methods ,Biochemistry ,Synthetic Genome Editing ,Genome Engineering ,Mice ,Heterochromatin ,Clustered Regularly Interspaced Short Palindromic Repeats ,DNA (Cytosine-5-)-Methyltransferases ,lcsh:Science ,RNA-Directed DNA Methylation ,Gene Editing ,Multidisciplinary ,DNA methylation ,Mammalian Genomics ,Crispr ,DNA (Cytosine-5-)-Methyltransferases/*metabolism ,Genomics ,Centromere/*genetics ,Chromatin ,Cell biology ,Enzymes ,Up-Regulation ,Nucleic acids ,Engineering and Technology ,Epigenetics ,Synthetic Biology ,DNA modification ,Chromatin modification ,Research Article ,Chromosome biology ,Biotechnology ,Imaging Techniques ,Epigenetic code ,Centromere ,Bioengineering ,Biology ,DNA, Satellite ,Research and Analysis Methods ,DNA methyltransferase ,Chromosomes ,Cell Line ,Up-Regulation/genetics ,03 medical and health sciences ,Fluorescence Imaging ,Epigenome editing ,Genetics ,Animals ,Embryo Implantation ,Biology and life sciences ,Base Sequence ,lcsh:R ,Embryos ,Proteins ,DNA ,Methyltransferases ,Synthetic Genomics ,DNA, Satellite/genetics ,030104 developmental biology ,Animal Genomics ,Synthetic Bioengineering ,Enzymology ,Illumina Methylation Assay ,lcsh:Q ,Gene expression ,Developmental Biology - Abstract
To study the impact of epigenetic changes on biological functions, the ability to manipulate the epigenetic status of certain genomic regions artificially could be an indispensable technology. "Epigenome editing" techniques have gradually emerged that apply TALE or CRISPR/Cas9 technologies with various effector domains isolated from epigenetic code writers or erasers such as DNA methyltransferase, 5-methylcytosine oxidase, and histone modification enzymes. Here we demonstrate that a TALE recognizing a major satellite, consisting of a repeated sequence in pericentromeres, could be fused with the bacterial CpG methyltransferase, SssI. ChIP-qPCR assays demonstrated that the fusion protein TALMaj-SssI preferentially bound to major chromosomal satellites in cultured cell lines. Then, TALMaj-SssI was expressed in fertilized mouse oocytes with hypomethylated major satellites (10-20% CpG islands). Bisulfite sequencing revealed that the DNA methylation status was increased specifically in major satellites (50-60%), but not in minor satellites or other repeat elements, such as Intracisternal A-particle (IAP) or long interspersed nuclear elements-1 (Line1) when the expression level of TALMaj-SssI is optimized in the cell. At a microscopic level, distal ends of chromosomes at the first mitotic stage were dramatically highlighted by the mCherry-tagged methyl CpG binding domain of human MBD1 (mCherry-MBD-NLS). Moreover, targeted DNA methylation to major satellites did not interfere with kinetochore function during early embryonic cleavages. Co-injection of dCas9 fused with SssI and guide RNA (gRNA) recognizing major satellite sequences enabled increment of the DNA methylation in the satellites, but a few off-target effects were also observed in minor satellites and retrotransposons. Although CRISPR can be applied instead of the TALE system, technical improvements to reduce off-target effects are required. We have demonstrated a new method of introducing DNA methylation without the need of other binding partners using the CpG methyltransferase, SssI.
- Published
- 2017
27. Estimating the disease burden of methicillin-resistant Staphylococcus aureus in Japan: Retrospective database study of Japanese hospitals
- Author
-
Hironori Uematsu, Kazuto Yamashita, Kiyohide Fushimi, Susumu Kunisawa, and Yuichi Imanaka
- Subjects
0301 basic medicine ,Male ,Pediatrics ,Databases, Factual ,Nosocomial Infections ,Staphylococcus ,lcsh:Medicine ,medicine.disease_cause ,Geographical Locations ,0302 clinical medicine ,Cost of Illness ,Japan ,Acute care ,Health care ,Infection control ,Public and Occupational Health ,030212 general & internal medicine ,Hospital Mortality ,Hospital Costs ,lcsh:Science ,Pathology and laboratory medicine ,Aged, 80 and over ,Multidisciplinary ,Pharmaceutics ,Middle Aged ,Staphylococcal Infections ,Medical microbiology ,Hospitalization ,Infectious Diseases ,Female ,Pathogens ,Research Article ,Drug Utilization ,Adult ,Methicillin-Resistant Staphylococcus aureus ,medicine.medical_specialty ,Staphylococcus aureus ,Drug Administration ,Asia ,Patients ,Infectious Disease Control ,030106 microbiology ,Surgical and Invasive Medical Procedures ,Microbiology ,03 medical and health sciences ,Drug Therapy ,medicine ,Humans ,Disease burden ,Aged ,Retrospective Studies ,Medicine and health sciences ,Inpatients ,Biology and life sciences ,Bacteria ,business.industry ,lcsh:R ,Organisms ,Retrospective cohort study ,Length of Stay ,Methicillin-resistant Staphylococcus aureus ,Microbial pathogens ,Health Care ,Emergency medicine ,People and Places ,lcsh:Q ,Bacterial pathogens ,Prospective payment system ,business - Abstract
Objectives The nationwide impact of antimicrobial-resistant infections on healthcare facilities throughout Japan has yet to be examined. This study aimed to estimate the disease burden of methicillin-resistant Staphylococcus aureus (MRSA) infections in Japanese hospitals. Design Retrospective analysis of inpatients comparing outcomes between subjects with and without MRSA infection. Data source A nationwide administrative claims database. Setting 1133 acute care hospitals throughout Japan. Participants All surgical and non-surgical inpatients who were discharged between April 1, 2014 and March 31, 2015. Main outcome measures Disease burden was assessed using hospitalization costs, length of stay, and in-hospital mortality. Using a unique method of infection identification, we categorized patients into an anti-MRSA drug group and a control group based on anti-MRSA drug utilization. To estimate the burden of MRSA infections, we calculated the differences in outcome measures between these two groups. The estimates were extrapolated to all 1584 acute care hospitals in Japan that have adopted a prospective payment system. Results We categorized 93 838 patients into the anti-MRSA drug group and 2 181 827 patients into the control group. The mean hospitalization costs, length of stay, and in-hospital mortality of the anti-MRSA drug group were US$33 548, 75.7 days, and 22.9%, respectively; these values were 3.43, 2.95, and 3.66 times that of the control group, respectively. When extrapolated to the 1584 hospitals, the total incremental burden of MRSA was estimated to be US$2 billion (3.41% of total hospitalization costs), 4.34 million days (3.02% of total length of stay), and 14.3 thousand deaths (3.62% of total mortality). Conclusions This study quantified the approximate disease burden of MRSA infections in Japan. These findings can inform policymakers on the burden of antimicrobial-resistant infections and support the application of infection prevention programs.
- Published
- 2017
28. AGIA Tag System Based on a High Affinity Rabbit Monoclonal Antibody against Human Dopamine Receptor D1 for Protein Analysis
- Author
-
Satoshi Yamanaka, Hiroyuki Takeda, Hirotaka Takahashi, Tomoya Yano, Keiichirou Nemoto, Takahiro Iwasaki, Atsushi Uematsu, Shunsuke Nomura, and Tatsuya Sawasaki
- Subjects
0301 basic medicine ,Antibody Affinity ,lcsh:Medicine ,Peptide ,Immunostaining ,Protein Sequencing ,Epitope ,Chromatography, Affinity ,Epitopes ,Mice ,0302 clinical medicine ,Protein sequencing ,Sequence Tagged Site Analysis ,Enzyme-Linked Immunoassays ,lcsh:Science ,Peptide sequence ,chemistry.chemical_classification ,Staining ,Multidisciplinary ,Antibodies, Monoclonal ,Precipitation Techniques ,Separation Processes ,Biochemistry ,030220 oncology & carcinogenesis ,MCF-7 Cells ,Rabbits ,Research Article ,Protein Binding ,medicine.drug_class ,Immunoblotting ,Molecular Probe Techniques ,Biology ,Monoclonal antibody ,Research and Analysis Methods ,03 medical and health sciences ,Affinity chromatography ,Cell Line, Tumor ,medicine ,Immunoprecipitation ,Animals ,Humans ,Amino Acid Sequence ,Immunoassays ,Molecular Biology Techniques ,Sequencing Techniques ,Molecular Biology ,Cell-free protein synthesis ,Receptors, Dopamine D1 ,lcsh:R ,Biology and Life Sciences ,Elution ,Molecular biology ,Co-Immunoprecipitation ,Peptide Fragments ,030104 developmental biology ,HEK293 Cells ,chemistry ,Specimen Preparation and Treatment ,Immunologic Techniques ,NIH 3T3 Cells ,lcsh:Q ,Myc-tag ,HeLa Cells - Abstract
Polypeptide tag technology is widely used for protein detection and affinity purification. It consists of two fundamental elements: a peptide sequence and a binder which specifically binds to the peptide tag. In many tag systems, antibodies have been used as binder due to their high affinity and specificity. Recently, we obtained clone Ra48, a high-affinity rabbit monoclonal antibody (mAb) against dopamine receptor D1 (DRD1). Here, we report a novel tag system composed of Ra48 antibody and its epitope sequence. Using a deletion assay, we identified EEAAGIARP in the C-terminal region of DRD1 as the minimal epitope of Ra48 mAb, and we named this sequence the “AGIA” tag, based on its central sequence. The tag sequence does not include the four amino acids, Ser, Thr, Tyr, or Lys, which are susceptible to post-translational modification. We demonstrated performance of this new tag system in biochemical and cell biology applications. SPR analysis demonstrated that the affinity of the Ra48 mAb to the AGIA tag was 4.90 × 10−9 M. AGIA tag showed remarkably high sensitivity and specificity in immunoblotting. A number of AGIA-fused proteins overexpressed in animal and plant cells were detected by anti-AGIA antibody in immunoblotting and immunostaining with low background, and were immunoprecipitated efficiently. Furthermore, a single amino acid substitution of the second Glu to Asp (AGIA/E2D) enabled competitive dissociation of AGIA/E2D-tagged protein by adding wild-type AGIA peptide. It enabled one-step purification of AGIA/E2D-tagged recombinant proteins by peptide competition under physiological conditions. The sensitivity and specificity of the AGIA system makes it suitable for use in multiple methods for protein analysis.
- Published
- 2016
29. Pulmonary Neoplasms in Patients with Birt-Hogg-Dubé Syndrome: Histopathological Features and Genetic and Somatic Events
- Author
-
Satoko Nakamura, Yoshiko Sudo, Hiroki Shirasaki, Yukio Nakatani, Shugo Uematsu, Kazuaki Yamada, Reiko Tanaka, Koji Okudela, Hiromu Yoshioka, Kazuhiro Yatera, Toshiaki Kunimura, Naoko Kimura, Mitsuko Furuya, Ikuma Kato, Ryo Shibuya, and Toyonori Tsuzuki
- Subjects
0301 basic medicine ,Male ,Pathology ,Lung Neoplasms ,DNA Mutational Analysis ,lcsh:Medicine ,Immunostaining ,Gene mutation ,medicine.disease_cause ,Pathology and Laboratory Medicine ,Birt–Hogg–Dubé syndrome ,Lung and Intrathoracic Tumors ,Birt-Hogg-Dube Syndrome ,0302 clinical medicine ,Papillary adenocarcinoma ,Neoplasms ,Adenocarcinomas ,Medicine and Health Sciences ,Phosphorylation ,lcsh:Science ,Staining ,Multidisciplinary ,Adenocarcinoma of the Lung ,TOR Serine-Threonine Kinases ,Middle Aged ,Oncology ,030220 oncology & carcinogenesis ,Adenocarcinoma ,Female ,KRAS ,Anatomy ,Research Article ,Signal Transduction ,medicine.medical_specialty ,Histology ,Molecular Sequence Data ,Adenocarcinoma of Lung ,Biology ,Research and Analysis Methods ,Carcinomas ,03 medical and health sciences ,Germline mutation ,Signs and Symptoms ,medicine ,Genetics ,Humans ,Atypical adenomatous hyperplasia ,Folliculin ,Germ-Line Mutation ,Aged ,Base Sequence ,lcsh:R ,Cancers and Neoplasms ,Biology and Life Sciences ,medicine.disease ,030104 developmental biology ,Specimen Preparation and Treatment ,Alveolar Epithelial Cells ,Mutation ,Lesions ,Somatic Mutation ,lcsh:Q - Abstract
Birt-Hogg-Dube syndrome (BHD) is an inherited disorder caused by genetic mutations in the folliculin (FLCN) gene. Individuals with BHD have multiple pulmonary cysts and are at a high risk for developing renal cell carcinomas (RCCs). Currently, little information is available about whether pulmonary cysts are absolutely benign or if the lungs are at an increased risk for developing neoplasms. Herein, we describe 14 pulmonary neoplastic lesions in 7 patients with BHD. All patients were confirmed to have germline FLCN mutations. Neoplasm histologies included adenocarcinoma in situ (n = 2), minimally invasive adenocarcinoma (n = 1), papillary adenocarcinoma (n = 1), micropapillary adenocarcinoma (n = 1), atypical adenomatous hyperplasia (n = 8), and micronodular pneumocyte hyperplasia (MPH)-like lesion (n = 1). Five of the six adenocarcinoma/MPH-like lesions (83.3%) demonstrated a loss of heterozygosity (LOH) of FLCN. All of these lesions lacked mutant alleles and preserved wild-type alleles. Three invasive adenocarcinomas possessed additional somatic events: 2 had a somatic mutation in the epidermal growth factor receptor gene (EGFR) and another had a somatic mutation in KRAS. Immunohistochemical analysis revealed that most of the lesions were immunostained for phospho-mammalian target of rapamycin (p-mTOR) and phospho-S6. Collective data indicated that pulmonary neoplasms of peripheral adenocarcinomatous lineage in BHD patients frequently exhibit LOH of FLCN with mTOR pathway signaling. Additional driver gene mutations were detected only in invasive cases, suggesting that FLCN LOH may be an underlying abnormality that cooperates with major driver gene mutations in the progression of pulmonary adenocarcinomas in BHD patients.
- Published
- 2016
30. Establishment of a Wheat Cell-Free Synthesized Protein Array Containing 250 Human and Mouse E3 Ubiquitin Ligases to Identify Novel Interaction between E3 Ligases and Substrate Proteins
- Author
-
Atsushi Uematsu, Satoshi Yamanaka, Tatsuya Sawasaki, Saki Yasuoka, Tatsuro Nakajima, Mei Imamura, Kousuke Doi, Chikako Takahashi, Hiroyuki Takeda, and Hirotaka Takahashi
- Subjects
0301 basic medicine ,Luminescence ,lcsh:Medicine ,Ubiquitin-conjugating enzyme ,F-box protein ,Biochemistry ,Substrate Specificity ,Mice ,Binding Analysis ,Post-Translational Modification ,lcsh:Science ,Triticum ,Plant Proteins ,Multidisciplinary ,biology ,Physics ,Electromagnetic Radiation ,Agriculture ,Plants ,Complementary DNA ,Recombinant Proteins ,Cell biology ,Ubiquitin ligase ,Precipitation Techniques ,Nucleic acids ,Immunoblot Analysis ,Wheat ,Physical Sciences ,Target protein ,Protein Binding ,Research Article ,Forms of DNA ,Ubiquitin-Protein Ligases ,Molecular Probe Techniques ,Crops ,Protein degradation ,Research and Analysis Methods ,Protein–protein interaction ,03 medical and health sciences ,Genetics ,Animals ,Humans ,Immunoprecipitation ,Grasses ,Molecular Biology Techniques ,Molecular Biology ,Chemical Characterization ,030102 biochemistry & molecular biology ,Cell-Free System ,lcsh:R ,Ubiquitination ,Organisms ,Biology and Life Sciences ,Proteins ,DNA ,Models, Theoretical ,Molecular biology ,Protein ubiquitination ,High-Throughput Screening Assays ,030104 developmental biology ,biology.protein ,lcsh:Q ,Crop Science ,Cereal Crops - Abstract
Ubiquitination is a key post-translational modification in the regulation of numerous biological processes in eukaryotes. The primary roles of ubiquitination are thought to be the triggering of protein degradation and the regulation of signal transduction. During protein ubiquitination, substrate specificity is mainly determined by E3 ubiquitin ligase (E3). Although more than 600 genes in the human genome encode E3, the E3s of many target proteins remain unidentified owing to E3 diversity and the instability of ubiquitinated proteins in cell. We demonstrate herein a novel biochemical analysis for the identification of E3s targeting specific proteins. Using wheat cell-free protein synthesis system, a protein array containing 227 human and 23 mouse recombinant E3s was synthesized. To establish the high-throughput binding assay using AlphaScreen technology, we selected MDM2 and p53 as the model combination of E3 and its target protein. The AlphaScreen assay specifically detected the binding of p53 and MDM2 in a crude translation mixture. Then, a comprehensive binding assay using the E3 protein array was performed. Eleven of the E3s showed high binding activity, including four previously reported E3s (e.g., MDM2, MDM4, and WWP1) targeting p53. This result demonstrated the reliability of the assay. Another interactors, RNF6 and DZIP3—which there have been no report to bind p53—were found to ubiquitinate p53 in vitro. Further analysis showed that RNF6 decreased the amount of p53 in H1299 cells in E3 activity-dependent manner. These results suggest the possibility that the RNF6 ubiquitinates and degrades p53 in cells. The novel in vitro screening system established herein is a powerful tool for finding novel E3s of a target protein.
- Published
- 2016
31. Injustice Experience Questionnaire, Japanese Version: Cross-Cultural Factor-Structure Comparison and Demographics Associated with Perceived Injustice
- Author
-
Akira Mibu, Hiroyasu Iso, Tomohiko Nishigami, Yasushi Motoyama, Keiko Yamada, Hironobu Uematsu, Kenichi Hayashi, Renzhe Cui, Tomonori Adachi, Hitoaki Sato, Yoichi Matsuda, Yumiko Takao, and Masahiko Shibata
- Subjects
Male ,Questionnaires ,Research Validity ,Culture ,Social Sciences ,lcsh:Medicine ,Pathology and Laboratory Medicine ,0302 clinical medicine ,Mathematical and Statistical Techniques ,Japan ,Sociology ,Surveys and Questionnaires ,Medicine and Health Sciences ,Public and Occupational Health ,030212 general & internal medicine ,lcsh:Science ,Reliability (statistics) ,Language ,Pain Measurement ,Analysis of covariance ,Multidisciplinary ,Middle Aged ,Research Assessment ,Research Design ,Physical Sciences ,symbols ,Regression Analysis ,Pain catastrophizing ,Female ,Safety ,Psychology ,Factor Analysis ,Statistics (Mathematics) ,Clinical psychology ,Research Article ,Adult ,Cross-Cultural Comparison ,Psychometrics ,Concurrent validity ,Pain ,Research and Analysis Methods ,03 medical and health sciences ,symbols.namesake ,Young Adult ,Signs and Symptoms ,Cronbach's alpha ,Social Justice ,Diagnostic Medicine ,Linear regression ,Cross-Cultural Studies ,Humans ,Brief Pain Inventory ,Statistical Methods ,Aged ,Demography ,Survey Research ,lcsh:R ,Traffic Safety ,Reproducibility of Results ,Translating ,Pearson product-moment correlation coefficient ,Abdominal Pain ,People and Places ,Perception ,lcsh:Q ,030217 neurology & neurosurgery ,Mathematics - Abstract
OBJECTIVE: The Injustice Experience Questionnaire (IEQ) assesses injury-related perceived injustice. This study aimed to (1) develop a Japanese version (IEQ-J), (2) examine its factor structure, validity, and reliability, and (3) discover which demographic variable(s) positively contributed to prediction of IEQ-J scores. METHODS: Data from 71 patients (33 male, 38 female; age = 20+) with injury pain were employed to investigate factor structure by exploratory and confirmatory factor analyses. Concurrent validity was examined by Pearson correlation coefficients among the IEQ-J, Brief Pain Inventory (BPI), and Pain Catastrophizing Scale (PCS). Internal consistency was investigated by Cronbach's alpha, and test-retest reliability was indicated with intra-class correlations (ICCs) in 42 of 71 patients within four weeks. Relations between demographic variables and IEQ-J scores were examined by covariance analysis and linear regression models. RESULTS: IEQ-J factor structure differed from the original two-factor model. A three-factor model with Severity/irreparability, Blame/unfairness, and Perceived lack of empathy was extracted. The three-factor model showed goodness-of-fit with the data and sufficient reliability (Cronbach's alpha of 0.90 for total IEQ-J; ICCs = 0.96). Pearson correlation coefficients among IEQ-J, BPI, and PCS ranged from 0.38 to 0.73. Pain duration over a year (regression coefficient, 11.92, 95%CI; 5.95-17.89) and liability for injury on another (regression coefficient, 12.17, 95%CI; 6.38-17.96) predicted IEQ-J total scores. CONCLUSIONS: This study evidenced the IEQ-J's sound psychometric properties. The three-factor model was the latter distinctive in the Japanese version. Pain duration over a year and injury liability by another statistically significantly increased IEQ-J scores. Language: en
- Published
- 2016
32. Prediction of pneumonia hospitalization in adults using health checkup data
- Author
-
Uematsu, Hironori, primary, Yamashita, Kazuto, additional, Kunisawa, Susumu, additional, Otsubo, Tetsuya, additional, and Imanaka, Yuichi, additional
- Published
- 2017
- Full Text
- View/download PDF
33. Estimating the disease burden of methicillin-resistant Staphylococcus aureus in Japan: Retrospective database study of Japanese hospitals
- Author
-
Uematsu, Hironori, primary, Yamashita, Kazuto, additional, Kunisawa, Susumu, additional, Fushimi, Kiyohide, additional, and Imanaka, Yuichi, additional
- Published
- 2017
- Full Text
- View/download PDF
34. The impact of patient profiles and procedures on hospitalization costs through length of stay in community-acquired pneumonia patients based on a Japanese administrative database
- Author
-
Kazuto Yamashita, Hironori Uematsu, Susumu Kunisawa, and Yuichi Imanaka
- Subjects
Male ,medicine.medical_specialty ,Databases, Factual ,Science ,medicine.medical_treatment ,Disease ,Communicable Diseases ,Structural equation modeling ,Japan ,Community-acquired pneumonia ,Acute care ,Health care ,Humans ,Medicine ,Intensive care medicine ,Aged ,Aged, 80 and over ,Mechanical ventilation ,Multidisciplinary ,Health economics ,business.industry ,Pneumonia ,Length of Stay ,medicine.disease ,Costs and Cost Analysis ,Female ,business ,Research Article - Abstract
BackgroundCommunity-acquired pneumonia is a common cause of patient hospitalization, and its burden on health care systems is increasing in aging societies. In this study, we aimed to investigate the factors that affect hospitalization costs in community-acquired pneumonia patients while considering the intermediate influence of patient length of stay.MethodsUsing a multi-institutional administrative claims database, we analyzed 30,041 patients hospitalized for community-acquired pneumonia who had been discharged between April 1, 2012 and September 30, 2013 from 289 acute care hospitals in Japan. Possible factors associated with hospitalization costs were investigated using structural equation modeling with length of stay as an intermediate variable. We calculated the direct, indirect (through length of stay), and total effects of the candidate factors on hospitalization costs in the model. Lastly, we calculated the ratio of indirect effects to direct effects for each factor.ResultsThe structural equation model showed that higher disease severities (using A-DROP, Barthel Index, and Charlson Comorbidity Index scores), use of mechanical ventilation, and tube feeding were associated with higher hospitalization costs, regardless of the intermediate influence of length of stay. The severity factors were also associated with longer length of stay durations. The ratio of indirect effects to direct effects on total hospitalization costs showed that the former was greater than the latter in the factors, except in the use of mechanical ventilation.ConclusionsOur structural equation modeling analysis indicated that patient profiles and procedures impacted on hospitalization costs both directly and indirectly. Furthermore, the profiles were generally shown to have greater indirect effects (through length of stay) on hospitalization costs than direct effects. These findings may be useful in supporting the more appropriate distribution of health care resources.
- Published
- 2015
35. Developmental Changes in the Corpus Callosum from Infancy to Early Adulthood: A Structural Magnetic Resonance Imaging Study
- Author
-
Kayoko Miura, Satoshi Uda, Chiaki Tanaka, Kyo Noguchi, Akiko Uematsu, Mie Matsui, Tomoko Sakai, and Megumi M. Tanaka-Arakawa
- Subjects
Multidisciplinary ,medicine.diagnostic_test ,lcsh:R ,Rostrum ,lcsh:Medicine ,Splenium ,Magnetic resonance imaging ,Anatomy ,Biology ,Corpus callosum ,medicine.anatomical_structure ,Cerebral cortex ,medicine ,lcsh:Q ,Young adult ,lcsh:Science ,Diffusion MRI ,Sex characteristics ,Research Article - Abstract
Previous research has reported on the development trajectory of the corpus callosum morphology. However, there have been only a few studies that have included data on infants. The goal of the present study was to examine the morphology of the corpus callosum in healthy participants of both sexes, from infancy to early adulthood. We sought to characterize normal development of the corpus callosum and possible sex differences in development. We performed a morphometric magnetic resonance imaging (MRI) study of 114 healthy individuals, aged 1 month to 25 years old, measuring the size of the corpus callosum. The corpus callosum was segmented into seven subareas of the rostrum, genu, rostral body, anterior midbody, posterior midbody, isthmus and splenium. Locally weighted regression analysis (LOESS) indicated significant non-linear age-related changes regardless of sex, particularly during the first few years of life. After this increase, curve slopes gradually became flat during adolescence and adulthood in both sexes. Age of local maximum for each subarea of the corpus callosum differed across the sexes. Ratios of total corpus callosum and genu, posterior midbody, as well as splenium to the whole brain were significantly higher in females compared with males. The present results demonstrate that the developmental trajectory of the corpus callosum during early life in healthy individuals is non-linear and dynamic. This pattern resembles that found for the cerebral cortex, further suggesting that this period plays a very important role in neural and functional development. In addition, developmental trajectories and changes in growth do show some sex differences.
- Published
- 2015
36. Prediction of pneumonia hospitalization in adults using health checkup data
- Author
-
Tetsuya Otsubo, Kazuto Yamashita, Yuichi Imanaka, Hironori Uematsu, and Susumu Kunisawa
- Subjects
Male ,Pulmonology ,Economics ,Physiology ,Social Sciences ,lcsh:Medicine ,Logistic regression ,Electrocardiography ,Eating ,Mathematical and Statistical Techniques ,Elderly ,0302 clinical medicine ,Japan ,Risk Factors ,Medicine and Health Sciences ,Public and Occupational Health ,030212 general & internal medicine ,lcsh:Science ,Multidisciplinary ,Middle Aged ,Vaccination and Immunization ,Community-Acquired Infections ,Hospitalization ,Vaccination ,Bioassays and Physiological Analysis ,Physical Sciences ,Female ,Statistics (Mathematics) ,Research Article ,Adult ,medicine.medical_specialty ,Immunology ,MEDLINE ,Research and Analysis Methods ,03 medical and health sciences ,Health Economics ,medicine ,Health insurance ,Humans ,Statistical Methods ,Intensive care medicine ,Aged ,Retrospective Studies ,business.industry ,Electrophysiological Techniques ,lcsh:R ,Biology and Life Sciences ,Retrospective cohort study ,Pneumonia ,medicine.disease ,Confidence interval ,Health Care ,030228 respiratory system ,Geriatrics ,Age Groups ,People and Places ,Emergency medicine ,Population Groupings ,lcsh:Q ,Preventive Medicine ,Cardiac Electrophysiology ,Physiological Processes ,business ,Mathematics ,Predictive modelling ,Forecasting ,Health Insurance - Abstract
Objectives Community-acquired pneumonia is a common cause of hospitalization, and pneumococcal vaccinations are recommended for high-risk individuals. Although risk factors for pneumonia have been identified, there are currently no pneumonia hospitalization prediction models based on the risk profiles of healthy subjects. This study aimed to develop a predictive model for pneumonia hospitalization in adults to accurately identify high-risk individuals to facilitate the efficient prevention of pneumonia. Methods We conducted a retrospective database analysis using health checkup data and health insurance claims data for residents of Kyoto prefecture, Japan, between April 2010 and March 2015. We chose adults who had undergone health checkups in the first year of the study period, and tracked pneumonia hospitalizations over the next 5 years. Subjects were randomly divided into training and test sets. The outcome measure was pneumonia hospitalization, and candidate predictors were obtained from the health checkup data. The prediction model was developed and internally validated using a LASSO logistic regression analysis. Lastly, we compared the new model with comparative models. Results The study sample comprised 54,907 people who had undergone health checkups. Among these, 921 were hospitalized for pneumonia during the study period. The c-statistic for the prediction model in the test set was 0.71 (95% confidence interval: 0.69-0.73). In contrast, a comparative model with only age and comorbidities as predictors had a lower c-statistic of 0.55 (95% confidence interval: 0.54-0.56). Conclusions Our predictive model for pneumonia hospitalization performed better than comparative models, and may be useful for supporting the development of pneumonia prevention measures.
- Published
- 2017
37. Prosaposin overexpression following kainic acid-induced neurotoxicity
- Author
-
Tetsuya Shimokawa, Hiroko Takechi, Takuya Doihara, Naoto Kobayashi, Keigo Uematsu, Shouichiro Saito, Kimiko Yamamiya, Hiroaki Nabeka, Seiji Matsuda, and Cheng Li
- Subjects
Male ,Pathology ,Excitotoxicity ,medicine.disease_cause ,Biochemistry ,Hippocampus ,Saposins ,chemistry.chemical_compound ,Neurotrophic factors ,GABAergic Neurons ,In Situ Hybridization ,gamma-Aminobutyric Acid ,Cerebral Cortex ,Neurons ,Kainic Acid ,Multidisciplinary ,Glutamate receptor ,Neurochemistry ,Up-Regulation ,Cell biology ,medicine.anatomical_structure ,Cerebral cortex ,Medicine ,Neurotoxicity Syndromes ,Research Article ,medicine.medical_specialty ,Kainic acid ,Science ,Neuroprotection ,Interneurons ,Seizures ,medicine ,Animals ,Rats, Wistar ,Prosaposin ,business.industry ,Neurotoxicity ,Biology and Life Sciences ,medicine.disease ,Rats ,Gene Expression Regulation ,Microscopy, Fluorescence ,chemistry ,nervous system ,Choroid Plexus ,Nerve Degeneration ,Lysosomes ,business ,Neuroscience - Abstract
Because excessive glutamate release is believed to play a pivotal role in numerous neuropathological disorders, such as ischemia or seizure, we aimed to investigate whether intrinsic prosaposin (PS), a neuroprotective factor when supplied exogenously in vivo or in vitro, is up-regulated after the excitotoxicity induced by kainic acid (KA), a glutamate analog. In the present study, PS immunoreactivity and its mRNA expression in the hippocampal and cortical neurons showed significant increases on day 3 after KA injection, and high PS levels were maintained even after 3 weeks. The increase in PS, but not saposins, detected by immunoblot analysis suggests that the increase in PS-like immunoreactivity after KA injection was not due to an increase in saposins as lysosomal enzymes after neuronal damage, but rather to an increase in PS as a neurotrophic factor to improve neuronal survival. Furthermore, several neurons with slender nuclei inside/outside of the pyramidal layer showed more intense PS mRNA expression than other pyramidal neurons. Based on the results from double immunostaining using anti-PS and anti-GABA antibodies, these neurons were shown to be GABAergic interneurons in the extra- and intra-pyramidal layers. In the cerebral cortex, several large neurons in the V layer showed very intense PS mRNA expression 3 days after KA injection. The choroid plexus showed intense PS mRNA expression even in the normal rat, and the intensity increased significantly after KA injection. The present study indicates that inhibitory interneurons as well as stimulated hippocampal pyramidal and cortical neurons synthesize PS for neuronal survival, and the choroid plexus is highly activated to synthesize PS, which may prevent neurons from excitotoxic neuronal damage. To the best of our knowledge, this is the first study that demonstrates axonal transport and increased production of neurotrophic factor PS after KA injection.
- Published
- 2014
38. Injustice Experience Questionnaire, Japanese Version: Cross-Cultural Factor-Structure Comparison and Demographics Associated with Perceived Injustice
- Author
-
Yamada, Keiko, primary, Adachi, Tomonori, additional, Mibu, Akira, additional, Nishigami, Tomohiko, additional, Motoyama, Yasushi, additional, Uematsu, Hironobu, additional, Matsuda, Yoichi, additional, Sato, Hitoaki, additional, Hayashi, Kenichi, additional, Cui, Renzhe, additional, Takao, Yumiko, additional, Shibata, Masahiko, additional, and Iso, Hiroyasu, additional
- Published
- 2016
- Full Text
- View/download PDF
39. AGIA Tag System Based on a High Affinity Rabbit Monoclonal Antibody against Human Dopamine Receptor D1 for Protein Analysis
- Author
-
Yano, Tomoya, primary, Takeda, Hiroyuki, additional, Uematsu, Atsushi, additional, Yamanaka, Satoshi, additional, Nomura, Shunsuke, additional, Nemoto, Keiichirou, additional, Iwasaki, Takahiro, additional, Takahashi, Hirotaka, additional, and Sawasaki, Tatsuya, additional
- Published
- 2016
- Full Text
- View/download PDF
40. Establishment of a Wheat Cell-Free Synthesized Protein Array Containing 250 Human and Mouse E3 Ubiquitin Ligases to Identify Novel Interaction between E3 Ligases and Substrate Proteins
- Author
-
Takahashi, Hirotaka, primary, Uematsu, Atsushi, additional, Yamanaka, Satoshi, additional, Imamura, Mei, additional, Nakajima, Tatsuro, additional, Doi, Kousuke, additional, Yasuoka, Saki, additional, Takahashi, Chikako, additional, Takeda, Hiroyuki, additional, and Sawasaki, Tatsuya, additional
- Published
- 2016
- Full Text
- View/download PDF
41. Pulmonary Neoplasms in Patients with Birt-Hogg-Dubé Syndrome: Histopathological Features and Genetic and Somatic Events
- Author
-
Furuya, Mitsuko, primary, Tanaka, Reiko, additional, Okudela, Koji, additional, Nakamura, Satoko, additional, Yoshioka, Hiromu, additional, Tsuzuki, Toyonori, additional, Shibuya, Ryo, additional, Yatera, Kazuhiro, additional, Shirasaki, Hiroki, additional, Sudo, Yoshiko, additional, Kimura, Naoko, additional, Yamada, Kazuaki, additional, Uematsu, Shugo, additional, Kunimura, Toshiaki, additional, Kato, Ikuma, additional, and Nakatani, Yukio, additional
- Published
- 2016
- Full Text
- View/download PDF
42. Developmental trajectories of amygdala and hippocampus from infancy to early adulthood in healthy individuals
- Author
-
Akiko Uematsu, Hisao Nishijo, Tsutomu Takahashi, Mie Matsui, Chiaki Tanaka, Michio Suzuki, and Kyo Noguchi
- Subjects
Male ,Aging ,Anatomy and Physiology ,Physiology ,Hippocampus ,lcsh:Medicine ,Developmental and Pediatric Neurology ,Hippocampal formation ,Pediatrics ,Functional Laterality ,Pregnancy ,Psychology ,Young adult ,Child ,lcsh:Science ,Sex Characteristics ,Multidisciplinary ,Organ Size ,Amygdala ,Mental Health ,medicine.anatomical_structure ,Neurology ,Health ,Child, Preschool ,Brain size ,Laterality ,Medicine ,Female ,Research Article ,Sex characteristics ,Adult ,medicine.medical_specialty ,Adolescent ,Neurophysiology ,Neuroimaging ,Biology ,Neurological System ,Young Adult ,Developmental Neuroscience ,medicine ,Humans ,Psychiatry ,lcsh:R ,Infant, Newborn ,Infant ,Sexual dimorphism ,Neuroanatomy ,nervous system ,Developmental Psychology ,lcsh:Q ,Neuroscience - Abstract
Knowledge of amygdalar and hippocampal development as they pertain to sex differences and laterality would help to understand not only brain development but also the relationship between brain volume and brain functions. However, few studies investigated development of these two regions, especially during infancy. The purpose of this study was to examine typical volumetric trajectories of amygdala and hippocampus from infancy to early adulthood by predicting sexual dimorphism and laterality. We performed a cross-sectional morphometric MRI study of amygdalar and hippocampal growth from 1 month to 25 years old, using 109 healthy individuals. The findings indicated significant non-linear age-related volume changes, especially during the first few years of life, in both the amygdala and hippocampus regardless of sex. The peak ages of amygdalar and hippocampal volumes came at the timing of preadolescence (9-11 years old). The female amygdala reached its peak age about one year and a half earlier than the male amygdala did. In addition, its rate of growth change decreased earlier in the females. Furthermore, both females and males displayed rightward laterality in the hippocampus, but only the males in the amygdala. The robust growth of the amygdala and hippocampus during infancy highlight the importance of this period for neural and functional development. The sex differences and laterality during development of these two regions suggest that sex-related factors such as sex hormones and functional laterality might affect brain development.
- Published
- 2012
43. Antibody-antigen-adjuvant conjugates enable co-delivery of antigen and adjuvant to dendritic cells in cis but only have partial targeting specificity
- Author
-
Sandra S. Diebold, Benoit Giquel, Satoshi Uematsu, Qin Hu, R.A. Abuknesha, Martin Kreutz, Frank O. Nestle, and Shizuo Akira
- Subjects
Cytotoxicity, Immunologic ,Immunoconjugates ,medicine.medical_treatment ,Cancer Treatment ,Priming (immunology) ,lcsh:Medicine ,Mice ,Drug Delivery Systems ,Immune Regulation [NCMLS 2] ,Neoplasms ,Cytotoxic T cell ,lcsh:Science ,Immune Response ,Multidisciplinary ,biology ,Immunizations ,Innate Immunity ,Tolerance induction ,Oligodeoxyribonucleotides ,Oncology ,Medicine ,Immunotherapy ,Antibody ,Adjuvant ,Research Article ,Tumor Immunology ,CpG Oligodeoxynucleotide ,Ovalbumin ,Immune Cells ,Molecular Sequence Data ,Immunology ,Antigen-Presenting Cells ,Receptors, Cell Surface ,Antibodies ,Minor Histocompatibility Antigens ,Cross-Priming ,Antigen ,Adjuvants, Immunologic ,Antigens, CD ,medicine ,Animals ,Lectins, C-Type ,Amino Acid Sequence ,Antigens ,Biology ,lcsh:R ,Immunity ,Dendritic Cells ,Immunologic Subspecialties ,Mice, Inbred C57BL ,CTL ,Solubility ,Toll-Like Receptor 9 ,Cancer research ,biology.protein ,lcsh:Q ,Peptides ,T-Lymphocytes, Cytotoxic - Abstract
Contains fulltext : 109503.pdf (Publisher’s version ) (Open Access) Antibody-antigen conjugates, which promote antigen-presentation by dendritic cells (DC) by means of targeted delivery of antigen to particular DC subsets, represent a powerful vaccination approach. To ensure immunity rather than tolerance induction the co-administration of a suitable adjuvant is paramount. However, co-administration of unlinked adjuvant cannot ensure that all cells targeted by the antibody conjugates are appropriately activated. Furthermore, antigen-presenting cells (APC) that do not present the desired antigen are equally strongly activated and could prime undesired responses against self-antigens. We, therefore, were interested in exploring targeted co-delivery of antigen and adjuvant in cis in form of antibody-antigen-adjuvant conjugates for the induction of anti-tumour immunity. In this study, we report on the assembly and characterization of conjugates consisting of DEC205-specific antibody, the model antigen ovalbumin (OVA) and CpG oligodeoxynucleotides (ODN). We show that such conjugates are more potent at inducing cytotoxic T lymphocyte (CTL) responses than control conjugates mixed with soluble CpG. However, our study also reveals that the nucleic acid moiety of such antibody-antigen-adjuvant conjugates alters their binding and uptake and allows delivery of the antigen and the adjuvant to cells partially independently of DEC205. Nevertheless, antibody-antigen-adjuvant conjugates are superior to antibody-free antigen-adjuvant conjugates in priming CTL responses and efficiently induce anti-tumour immunity in the murine B16 pseudo-metastasis model. A better understanding of the role of the antibody moiety is required to inform future conjugate vaccination strategies for efficient induction of anti-tumour responses.
- Published
- 2012
44. Expression and Characterization of Recombinant, Tetrameric and Enzymatically Active Influenza Neuraminidase for the Setup of an Enzyme-Linked Lectin-Based Assay
- Author
-
Prevato, Marua, primary, Ferlenghi, Ilaria, additional, Bonci, Alessandra, additional, Uematsu, Yasushi, additional, Anselmi, Giulia, additional, Giusti, Fabiola, additional, Bertholet, Sylvie, additional, Legay, Francois, additional, Telford, John Laird, additional, Settembre, Ethan C., additional, Maione, Domenico, additional, and Cozzi, Roberta, additional
- Published
- 2015
- Full Text
- View/download PDF
45. Population Structure of and Conservation Strategies for Wild Pyrus ussuriensis Maxim. in China
- Author
-
Wuyun, Tana, primary, Amo, Hitomi, additional, Xu, Jingshi, additional, Ma, Teng, additional, Uematsu, Chiyomi, additional, and Katayama, Hironori, additional
- Published
- 2015
- Full Text
- View/download PDF
46. The Impact of Patient Profiles and Procedures on Hospitalization Costs through Length of Stay in Community-Acquired Pneumonia Patients Based on a Japanese Administrative Database
- Author
-
Uematsu, Hironori, primary, Kunisawa, Susumu, additional, Yamashita, Kazuto, additional, and Imanaka, Yuichi, additional
- Published
- 2015
- Full Text
- View/download PDF
47. Developmental Changes in the Corpus Callosum from Infancy to Early Adulthood: A Structural Magnetic Resonance Imaging Study
- Author
-
Tanaka-Arakawa, Megumi M., primary, Matsui, Mie, additional, Tanaka, Chiaki, additional, Uematsu, Akiko, additional, Uda, Satoshi, additional, Miura, Kayoko, additional, Sakai, Tomoko, additional, and Noguchi, Kyo, additional
- Published
- 2015
- Full Text
- View/download PDF
48. Amyloid β-Mediated Zn2+ Influx into Dentate Granule Cells Transiently Induces a Short-Term Cognitive Deficit
- Author
-
Takeda, Atsushi, primary, Nakamura, Masatoshi, additional, Fujii, Hiroaki, additional, Uematsu, Chihiro, additional, Minamino, Tatsuya, additional, Adlard, Paul A., additional, Bush, Ashley I., additional, and Tamano, Haruna, additional
- Published
- 2014
- Full Text
- View/download PDF
49. Prosaposin Overexpression following Kainic Acid-Induced Neurotoxicity
- Author
-
Nabeka, Hiroaki, primary, Uematsu, Keigo, additional, Takechi, Hiroko, additional, Shimokawa, Tetsuya, additional, Yamamiya, Kimiko, additional, Li, Cheng, additional, Doihara, Takuya, additional, Saito, Shouichiro, additional, Kobayashi, Naoto, additional, and Matsuda, Seiji, additional
- Published
- 2014
- Full Text
- View/download PDF
50. Extracellular and mixotrophic symbiosis in the whale-fall mussel Adipicola pacifica: a trend in evolution from extra- to intracellular symbiosis
- Author
-
Yoshihiro Fujiwara, Katsuyuki Uematsu, Toshiro Yamanaka, Yuko Fujita, Gin Kinoshita, Chikayo Noda, Masaru Kawato, and Jun-Ichi Miyazaki
- Subjects
Gill ,Gills ,Science ,Molecular Sequence Data ,Models, Biological ,Polymerase Chain Reaction ,Ecology/Marine and Freshwater Ecology ,Symbiosis ,Marine and Aquatic Sciences/Microbiology ,RNA, Ribosomal, 16S ,Whale fall ,Ecology/Evolutionary Ecology ,Extracellular ,Animals ,Marine and Aquatic Sciences/Evolutionary Biology ,In Situ Hybridization, Fluorescence ,Phylogeny ,Evolutionary Biology ,Multidisciplinary ,biology ,Bacteria ,Ecology ,Mussel ,Bivalvia ,biology.organism_classification ,Biological Evolution ,Cell Biology/Cell Adhesion ,Medicine ,Mixotroph ,Ecology/Environmental Microbiology ,Hydrothermal vent ,Research Article - Abstract
BackgroundDeep-sea mussels harboring chemoautotrophic symbionts from hydrothermal vents and seeps are assumed to have evolved from shallow-water asymbiotic relatives by way of biogenic reducing environments such as sunken wood and whale falls. Such symbiotic associations have been well characterized in mussels collected from vents, seeps and sunken wood but in only a few from whale falls.Methodology/principal findingHere we report symbioses in the gill tissues of two mussels, Adipicola crypta and Adipicola pacifica, collected from whale-falls on the continental shelf in the northwestern Pacific. The molecular, morphological and stable isotopic characteristics of bacterial symbionts were analyzed. A single phylotype of thioautotrophic bacteria was found in A. crypta gill tissue and two distinct phylotypes of bacteria (referred to as Symbiont A and Symbiont C) in A. pacifica. Symbiont A and the A. crypta symbiont were affiliated with thioautotrophic symbionts of bathymodiolin mussels from deep-sea reducing environments, while Symbiont C was closely related to free-living heterotrophic bacteria. The symbionts in A. crypta were intracellular within epithelial cells of the apical region of the gills and were extracellular in A. pacifica. No spatial partitioning was observed between the two phylotypes in A. pacifica in fluorescence in situ hybridization experiments. Stable isotopic analyses of carbon and sulfur indicated the chemoautotrophic nature of A. crypta and mixotrophic nature of A. pacifica. Molecular phylogenetic analyses of the host mussels showed that A. crypta constituted a monophyletic clade with other intracellular symbiotic (endosymbiotic) mussels and that A. pacifica was the sister group of all endosymbiotic mussels.Conclusions/significanceThese results strongly suggest that the symbiosis in A. pacifica is at an earlier stage in evolution than other endosymbiotic mussels. Whale falls and other modern biogenic reducing environments may act as refugia for primal chemoautotrophic symbioses between eukaryotes and prokaryotes since the extinction of ancient large marine vertebrates.
- Published
- 2009
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.