1. Genomic surveillance of SARS-CoV-2 Spike gene by sanger sequencing.
- Author
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Salles TS, Cavalcanti AC, da Costa FB, Dias VZ, de Souza LM, de Meneses MDF, da Silva JAS, Amaral CD, Felix JR, Pereira DA, Boatto S, Guimarães MAAM, Ferreira DF, and Azevedo RC
- Subjects
- Base Sequence, Brazil epidemiology, COVID-19 virology, Diagnostic Tests, Routine methods, Electrophoresis, Agar Gel methods, Epidemiological Monitoring, Humans, Mutation, RNA, Viral genetics, RNA, Viral isolation & purification, COVID-19 diagnosis, COVID-19 epidemiology, COVID-19 Nucleic Acid Testing methods, Genes, Viral, Pandemics prevention & control, Reverse Transcriptase Polymerase Chain Reaction methods, SARS-CoV-2 genetics, Sequence Analysis, RNA methods, Spike Glycoprotein, Coronavirus genetics
- Abstract
The SARS-CoV-2 responsible for the ongoing COVID pandemic reveals particular evolutionary dynamics and an extensive polymorphism, mainly in Spike gene. Monitoring the S gene mutations is crucial for successful controlling measures and detecting variants that can evade vaccine immunity. Even after the costs reduction resulting from the pandemic, the new generation sequencing methodologies remain unavailable to a large number of scientific groups. Therefore, to support the urgent surveillance of SARS-CoV-2 S gene, this work describes a new feasible protocol for complete nucleotide sequencing of the S gene using the Sanger technique. Such a methodology could be easily adopted by any laboratory with experience in sequencing, adding to effective surveillance of SARS-CoV-2 spreading and evolution., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2022
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