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Saturated fatty acids modulate autophagy's proteins in the hypothalamus.
- Source :
-
PloS one [PLoS One] 2015 Mar 18; Vol. 10 (3), pp. e0119850. Date of Electronic Publication: 2015 Mar 18 (Print Publication: 2015). - Publication Year :
- 2015
-
Abstract
- Autophagy is an important process that regulates cellular homeostasis by degrading dysfunctional proteins, organelles and lipids. In this study, the hypothesis that obesity could lead to impairment in hypothalamic autophagy in mice was evaluated by examining the hypothalamic distribution and content of autophagic proteins in animal with obesity induced by 8 or 16 weeks high fat diet to induce obesity and in response to intracerebroventricular injections of palmitic acid. The results showed that chronic exposure to a high fat diet leads to an increased expression of inflammatory markers and downregulation of autophagic proteins. In obese mice, autophagic induction leads to the downregulation of proteins, such as JNK and Bax, which are involved in the stress pathways. In neuron cell-line, palmitate has a direct effect on autophagy even without inflammatory activity. Understanding the cellular and molecular bases of overnutrition is essential for identifying new diagnostic and therapeutic targets for obesity.
- Subjects :
- Analysis of Variance
Animals
Cell Line
Fluorescent Antibody Technique
Glucose Tolerance Test
Hypothalamus metabolism
Immunoblotting
MAP Kinase Kinase 4 metabolism
Male
Mice
Mice, Obese
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
bcl-2-Associated X Protein metabolism
Animal Nutritional Physiological Phenomena
Autophagy physiology
Fatty Acids metabolism
Hypothalamus physiology
Obesity physiopathology
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 10
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 25786112
- Full Text :
- https://doi.org/10.1371/journal.pone.0119850