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A new approach for heparin standardization: combination of scanning UV spectroscopy, nuclear magnetic resonance and principal component analysis.

Authors :
Lima MA
Rudd TR
de Farias EH
Ebner LF
Gesteira TF
de Souza LM
Mendes A
Córdula CR
Martins JR
Hoppensteadt D
Fareed J
Sassaki GL
Yates EA
Tersariol IL
Nader HB
Source :
PloS one [PLoS One] 2011 Jan 18; Vol. 6 (1), pp. e15970. Date of Electronic Publication: 2011 Jan 18.
Publication Year :
2011

Abstract

The year 2007 was marked by widespread adverse clinical responses to heparin use, leading to a global recall of potentially affected heparin batches in 2008. Several analytical methods have since been developed to detect impurities in heparin preparations; however, many are costly and dependent on instrumentation with only limited accessibility. A method based on a simple UV-scanning assay, combined with principal component analysis (PCA), was developed to detect impurities, such as glycosaminoglycans, other complex polysaccharides and aromatic compounds, in heparin preparations. Results were confirmed by NMR spectroscopy. This approach provides an additional, sensitive tool to determine heparin purity and safety, even when NMR spectroscopy failed, requiring only standard laboratory equipment and computing facilities.

Details

Language :
English
ISSN :
1932-6203
Volume :
6
Issue :
1
Database :
MEDLINE
Journal :
PloS one
Publication Type :
Academic Journal
Accession number :
21267460
Full Text :
https://doi.org/10.1371/journal.pone.0015970