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Your search keyword '"Stephan Niemann"' showing total 2 results
Start Over Author "Stephan Niemann" Journal neuroscience letters
2 results on '"Stephan Niemann"'

1. Identification of shyc, a novel gene expressed in the murine developing and adult nervous system

Author

Irm Hermans-Borgmeyer, Birgitta Schinke, Stephan Niemann, and Frank Köster

Subjects
Nervous system, Embryonal Carcinoma Stem Cells, Molecular Sequence Data, Central nervous system, Nerve Tissue Proteins, In situ hybridization, Biology, Embryonal carcinoma, Mice, Complementary DNA, Gene expression, medicine, Animals, Amino Acid Sequence, In Situ Hybridization, Adaptor Proteins, Signal Transducing, Brain Chemistry, cDNA library, General Neuroscience, Age Factors, Brain, Gene Expression Regulation, Developmental, medicine.disease, Cell biology, medicine.anatomical_structure, P19 cell, Immunology, Neoplastic Stem Cells
Abstract

The embryonal carcinoma cell line P19 responds to treatment with retinoid acid by differentiation into neuronal cell types [2]. Using radioactively labeled cDNA derived from differentiating P19 cells we screened an adult mouse brain cDNA library and isolated a gene named shyc for selective hybridizing clone. The encoded protein did not reveal homology to any known protein. We used in situ hybridization on mouse embryonic and adult brain sections to study shyc expression. The developing and embryonic nervous system showed the most prominent hybridization signals. In the adult brain the olfactory pathway was marked by shyc expression.

Published
1998

2. Head-activator and the neuroectodermal differentiation of P19 mouse embryonal carcinoma cells

Author

Stephan Niemann and H. Chica Schaller

Subjects
medicine.medical_specialty, Ratón, Retinoic acid, Neuropeptide, Fluorescent Antibody Technique, Tretinoin, Biology, Calcium in biology, Embryonal carcinoma, chemistry.chemical_compound, Mice, Internal medicine, medicine, Tumor Cells, Cultured, Animals, Activator (genetics), General Neuroscience, Neuropeptides, Cell Differentiation, medicine.disease, Molecular biology, In vitro, Pyrrolidonecarboxylic Acid, Endocrinology, P19 cell, chemistry, embryonic structures, Calcium, Cell Division
Abstract

P19 embryonal carcinoma cells differentiate into neuroectodermal derivates upon aggregation and treatment with retinoic acid (RA). Such RA-induced P19 cells, concomitant with neuronal differentiation, produce the neuropeptide head activator (HA) and release HA into the culture medium. The amount of HA produced by such differentiating P19 cells is more than 100-fold higher than that of uninduced P19 cells. RA-induced P19 cells respond to HA treatment with a two-fold increase in total number of neuronally differentiating P19 progeny. As early response to HA, an increase in intracellular calcium concentration is observed.

Published
1996

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