1. Bioactive DNA-Peptide Nanotubes Enhance the Differentiation of Neural Stem Cells Into Neurons
- Author
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Ronit Freeman, John A. Kessler, Samuel I. Stupp, Shantanu Sur, Su Ji Jeong, Nicholas Stephanopoulos, Hilary A. North, and Faifan Tantakitti
- Subjects
Nanotubes, Peptide ,Letter ,Materials science ,Base pair ,extracellular matrix ,Cellular differentiation ,Bioengineering ,Peptide ,Extracellular matrix ,Mice ,chemistry.chemical_compound ,Neural Stem Cells ,DNA nanotechnology ,Animals ,General Materials Science ,Cell adhesion ,Cells, Cultured ,Neurons ,chemistry.chemical_classification ,Mechanical Engineering ,Cell Differentiation ,DNA ,Self-assembly ,General Chemistry ,Condensed Matter Physics ,DNA-peptide ,Molecular biology ,Neural stem cell ,Cell biology ,chemistry ,Oligopeptides ,biomaterials - Abstract
We report the construction of DNA nanotubes covalently functionalized with the cell adhesion peptide RGDS as a bioactive substrate for neural stem cell differentiation. Alteration of the Watson–Crick base pairing program that builds the nanostructures allowed us to probe independently the effect of nanotube architecture and peptide bioactivity on stem cell differentiation. We found that both factors instruct synergistically the preferential differentiation of the cells into neurons rather than astrocytes.
- Published
- 2014