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Amplified Multiplexed Analysis of DNA by the Exonuclease III-Catalyzed Regeneration of the Target DNA in the Presence of Functionalized Semiconductor Quantum Dots.
- Source :
-
Nano Letters . Oct2011, Vol. 11 Issue 10, p4456-4461. 6p. - Publication Year :
- 2011
-
Abstract
- Quantum dots (QDs) functionalized with a black-hole quencher are used as optical tracer for the detection of DNA using exonuclease as a biocatalyst. The binding of the target DNA or of a target/open hairpin complex to the functionalized QDs leads to the exonuclease-stimulated recycling of the target DNA or the target/hairpin complex. This results in the triggering of the luminescence of the QDs that provides a readout signal for the amplified sensing process. By using different-sized QDs, the multiplexed detection of DNAs is demonstrated. [ABSTRACT FROM AUTHOR]
- Subjects :
- *DNA
*EXONUCLEASES
*CATALYSIS
*REGENERATION (Biology)
*QUANTUM dots
*LUMINESCENCE
Subjects
Details
- Language :
- English
- ISSN :
- 15306984
- Volume :
- 11
- Issue :
- 10
- Database :
- Academic Search Index
- Journal :
- Nano Letters
- Publication Type :
- Academic Journal
- Accession number :
- 66648492
- Full Text :
- https://doi.org/10.1021/nl202761g