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A total of 12 abietane diterpenoids were isolated and identified from Rosmarinus officinalis in which 6 ones were undescribed compounds. Their structures were illuminated by the HRESIMS, NMR, and ECD methods and named as rosmarinusin Q-V (1 – 6). It worthy mentioned that rosmarinusin Q was a novel abietane diterpenoid with 6/6/5 skeleton whose C ring was an α , β -unsaturated five-element ketone. All the compounds and four compounds (13 – 16) reported in our previous paper were evaluated their anti-neuroinflammatory activities on the LPS-induced BV2 cells. Compounds 5 , 8 , 9 , 11 , and 15 displayed significant anti-neuroinflammatory activity at the concentration of 10, 20, and 40 μM respectively. These results confirmed that R. officinalis contained abundant abietane diterpenoids and these compounds showed potential values of anti-neuroinflammation which could be developed as neuroprotective agents for the treatment of nerve damage caused by inflammation. [Display omitted] [ABSTRACT FROM AUTHOR]

Abstract Diterpenoids salvimulticanol (1) and salvimulticaoic acid (2) together with known diterpenoid (3–6) were isolated from Salvia multicaulis. Structures were elucidated by spectroscopic techniques including HRESIMS as well as 1D-, and 2D-NMR. In-vitro cytotoxicity was assayed against human cancer cell lines. As several metabolites exhibited activity against drug-resistance lines, compounds were screened against a panel of human drug-sensitive and multidrug-resistant cancer lines. A proposed biosynthetic pathway for these new diterpenoids (1 – 2) as well as the cytotoxic structure-activity relationship of all identified compounds were discussed. Compound 1 and 6 showed the most potent cytotoxicity with IC 50 11.58 and 4.13 towards leukemia cell lines CCRF-CEM and CEM-ADR5000, respectively. Graphical abstract Unlabelled Image [ABSTRACT FROM AUTHOR]

A phytochemical investigation of the root extracts of Salvia tebesana Bunge led to the isolation of two new diterpenoids, tebesinone A ( 1 ) and tebesinone B ( 2 ), along with two known compounds, aegyptinone A ( 3 ) and aegyptinone B ( 4 ). The structures were established by spectroscopic analysis including 1D and 2D NMR and HRESIMS. The cytotoxic activities of the crude extracts and isolated compounds were evaluated against MCF-7, B16F10, PC-3 and C26 human cancer cell lines, in which compounds 2 and 3 showed significant cytotoxic activities (IC 50 2.1–10.3 μM). [ABSTRACT FROM AUTHOR]

A phytochemical investigation of the leaves of Callicarpa kwangtungensis led to the isolation of three new diterpenoids ( 1 – 3 ), callipenes A–C, and eleven known analogues ( 4 – 14 ). Their structures were established on the basis of extensive analysis of NMR spectroscopic data, X-ray diffraction data, and experimental and calculated electronic circular dichroism spectra. Compounds 1 and 2 are rare abietane diterpenoids possessing a peroxide bridge. All of the isolates were found to inhibit LPS-induced NO production in BV-2 cells. [ABSTRACT FROM AUTHOR]

Five new abietane diterpenoids, named abifadines A–E ( 1 – 5 ), and nine known ones ( 6 – 14 ), together with two known podocarpenes ( 15 and 16 ), were isolated from the branch and leaf of Abies fargesii . Their structures including the relative configurations were established on the basis of extensive spectroscopic analyses. The absolute configuration of 1 was determined through single-crystal X-ray diffraction analysis with Cu K α irradiation. All the isolates were evaluated for their in vitro cytotoxicities. Only compounds 5 and 12 exhibited weak anti-proliferative effects against three cancer cell lines (B16, MCF7, and HepG2) with IC 50 values range from 14.8 to 42.8 μM. The antimicrobial activities against Staphylococcus aureus, Escherichia coli and Monilia albicans of all compounds were also tested, but none of them showed significant activities. [ABSTRACT FROM AUTHOR]

Four new aromatic abietane diterpenoids and two new benzene derivatives, namely perenacidins A–F ( 1 – 6 ), have been isolated from the fruiting bodies of Basidiomycete Perenniporia subacida . The structures were elucidated by means of extensive spectroscopic methods and computational ECD method. The antifungal activities against Canidia albicans and the cytotoxic activities against four cancer cell lines (including K-562, A-549, SMMC-7721, MCF-7) were evaluated in vitro . [ABSTRACT FROM AUTHOR]

Two new abietane diterpenoids, Gerardianin B ( 1 ) and Gerardianin C ( 2 ), one new lignan glycoside, Gerardianin D ( 3 ) and one new lupane-type triterpenoid, Gerardianol A ( 4 ), together with seven known abietane diterpenoids were isolated from the aerial parts of Rabdosia lophanthoides var. gerardianus. Their structures were determined by 1D and 2D NMR spectroscopic data. The cytotoxic activities of the nine diterpenoids were evaluated on human cancer cell lines. Compounds 6–11 exhibited significant cytotoxic activities against HepG2 cell lines with IC 50 from 4.68 to 9.43 μM and HCF-8 cell lines with IC 50 from 9.12 to 13.53 μM. [ABSTRACT FROM AUTHOR]

Two previously undescribed diterpenes (1 and 2), as well as one curious triterpenoid were isolated from Clinopodium polycephalum , a medicinal plant distributed in southwestern and eastern China. Their structures were elucidated using MS analyses, UV spectrum, and extensive 2D-homo and heteronuclear NMR data interpretations. Among them, 1 had an unusual skeletal characteristic produced by a rare methyl migration pathway. All monomer compounds exhibited inhibitory effects on NO production in LPS-induced RAW 264.7 cells without affecting cell viabilities, which were comparable to that of positive control. [Display omitted] • Three new (1 – 3) and five known compounds were isolated from Clinopodium polycephalum. • Structures were characterized by 1D and 2D NMR, HRESIMS, ECD analysis and DP4+ analysis. • Compounds 1 – 8 exhibited a significant inhibitory effect against NO production in LPS-stimulated RAW 264.7 macrophages. • The hypothetical biosynthetic pathway for compound 1 was proposed. [ABSTRACT FROM AUTHOR]

Phytochemical investigation of the ethyl acetate extract of Lycopodiastrum casuarinoides (Spring) Holub (Lycopodiaceae) led to the isolation of nine compounds, including two new serratene triterpenoids, serrat-14-en-3α,21α-diol (1), 26-nor-8-oxo-21-one-α-onocerin (6), one new abietane diterpenoid, lycocasuarinone A (7), one new sesquiterpene acid, 7, 9-diene-1,4-epoxy-2-hydroxy-10-carboxylic acid (8) and one new chromone derivative, 5,7-dihydroxy-2-methyl esterchromone (9), together with four known serratene triterpenoids (2 – 5). Abietane diterpenoid (7) and sesquiterpene acid (8) from Lycopodiastrum casuarinoides are reported for the first time. Their structures and stereochemistry were unambiguously elucidated by spectroscopic analysis and comparison with known ones. All the compounds were tested for acetylcholinesterase (AChE) and butyrocholinesterase (BuChE) inhibitory activities. Bioactivity assays revealed that compound 6 exhibited the most potent AChE inhibitory effect. Unlabelled Image [ABSTRACT FROM AUTHOR]