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Start Over Author "H. Bernon" Journal clinical chemistry
4 results on '"H. Bernon"'

1. Multicenter evaluation of the Paragon CZE 2000 capillary zone electrophoresis system for serum protein electrophoresis and monoclonal component typing

Author

J, Bienvenu, M S, Graziani, F, Arpin, H, Bernon, C, Blessum, C, Marchetti, G, Righetti, M, Somenzini, G, Verga, and F, Aguzzi

Subjects
Quality Control, Nephrotic Syndrome, Antibodies, Monoclonal, Electrophoresis, Capillary, Fibrinogen, Reproducibility of Results, Bilirubin, Blood Proteins, Electrophoresis, Cellulose Acetate, Sensitivity and Specificity, Hemoglobins, Nephelometry and Turbidimetry, Reference Values, Humans, Serum Globulins, gamma-Globulins, Laboratories, Immunoelectrophoresis, Serum Albumin, Triglycerides
Abstract

Serum protein electrophoresis and typing of monoclonal components (MCs) are routine but time-consuming and technically demanding assays. We evaluated capillary electrophoresis (Paragon CZE 2000) for automation of the two assays. CZE and cellulose acetate electrophoresis gave similar data on 794 samples. Within-run and between-run CVs were2% for albumin and gamma-globulins and 4-7% for alpha 1-, alpha 2-, and beta-globulins. Bilirubin, hemoglobin, triglycerides, and fibrinogen were found not to interfere. No carryover by capillaries was detected. The detection limit for MC was0.5 g/L. MC assessment by immunosubtraction on 403 samples identified the monoclonal type in all samples with peak concentrations10 g/L; only 50% of MCs that could not be quantified by densitometric scan were typed.

Published
1998

2. Use of immunoglobulin heavy-chain and light-chain measurements in a multicenter trial to investigate monoclonal components: I. Detection

Author

R G, Jones, F, Aguzzi, J, Bienvenu, P, Bianchi, C, Gasparro, M R, Bergami, A, Perinet, H, Bernon, G M, Penn, and I, Keller

Subjects
Electrophoresis, Agar Gel, Male, Quality Control, Paraproteinemias, Antibodies, Monoclonal, Immunoglobulin A, Immunoglobulin kappa-Chains, Immunoglobulin M, Immunoglobulin lambda-Chains, Nephelometry and Turbidimetry, Immunoglobulin G, Humans, Female, Immunoglobulin Light Chains, Immunoglobulin Heavy Chains, Immunoelectrophoresis
Abstract

We assessed the combined use of serum protein electrophoresis (SPE) and nephelometric measurement of immunoglobulin heavy- and light-chain components for detecting serum monoclonal immunoglobulins (monoclonal components, MC) in 4788 unselected samples from 4173 patients. MC were detected in 514 samples from 390 patients. In 356 these were detected by SPE; the other 34 had a normal SPE pattern but an abnormal kappa:lambda light-chain ratio (KLR). Only 208 of the 356 (58%) samples with bands by SPE had abnormal KLRs. Samples with MC concentrations greater than 5 g/L had a higher proportion of abnormal KLRs (75%) than those with concentrations less than 5 g/L (42%). The KLR was abnormal in 13% of samples in which no MC were visible by SPE or immunofixation electrophoresis (IFE). Compared with quantitative measurements of immunoglobulin heavy and light chains, high-quality SPE remains the method of choice for the detection of MC. Quantitative methods, however, are able to detect additional MC, especially those containing free light chains, and in the absence of SPE and IFE will detect about 75% of MC present at greater than 5 g/L.

Published
1991

3. Multicenter evaluation of the Paragon CZE 2000 capillary zone electrophoresis system for serum protein electrophoresis and monoclonal component typing.

Author

Bienvenu J, Graziani MS, Arpin F, Bernon H, Blessum C, Marchetti C, Righetti G, Somenzini M, Verga G, and Aguzzi F

Subjects
Bilirubin blood, Electrophoresis, Capillary methods, Electrophoresis, Cellulose Acetate methods, Fibrinogen, Hemoglobins, Humans, Immunoelectrophoresis methods, Laboratories standards, Nephelometry and Turbidimetry methods, Nephrotic Syndrome blood, Quality Control, Reference Values, Reproducibility of Results, Sensitivity and Specificity, Serum Albumin isolation & purification, Serum Globulins isolation & purification, Triglycerides blood, gamma-Globulins isolation & purification, Antibodies, Monoclonal, Blood Proteins isolation & purification, Electrophoresis, Capillary instrumentation
Abstract

Serum protein electrophoresis and typing of monoclonal components (MCs) are routine but time-consuming and technically demanding assays. We evaluated capillary electrophoresis (Paragon CZE 2000) for automation of the two assays. CZE and cellulose acetate electrophoresis gave similar data on 794 samples. Within-run and between-run CVs were < 2% for albumin and gamma-globulins and 4-7% for alpha 1-, alpha 2-, and beta-globulins. Bilirubin, hemoglobin, triglycerides, and fibrinogen were found not to interfere. No carryover by capillaries was detected. The detection limit for MC was < 0.5 g/L. MC assessment by immunosubtraction on 403 samples identified the monoclonal type in all samples with peak concentrations > 10 g/L; only 50% of MCs that could not be quantified by densitometric scan were typed.

Published
1998

4. Use of immunoglobulin heavy-chain and light-chain measurements in a multicenter trial to investigate monoclonal components: I. Detection.

Author

Jones RG, Aguzzi F, Bienvenu J, Bianchi P, Gasparro C, Bergami MR, Perinet A, Bernon H, Penn GM, and Keller I

Subjects
Antibodies, Monoclonal analysis, Electrophoresis, Agar Gel, Female, Humans, Immunoelectrophoresis, Immunoglobulin A analysis, Immunoglobulin G analysis, Immunoglobulin M analysis, Immunoglobulin kappa-Chains analysis, Immunoglobulin lambda-Chains analysis, Male, Nephelometry and Turbidimetry, Paraproteinemias immunology, Quality Control, Immunoglobulin Heavy Chains analysis, Immunoglobulin Light Chains analysis
Abstract

We assessed the combined use of serum protein electrophoresis (SPE) and nephelometric measurement of immunoglobulin heavy- and light-chain components for detecting serum monoclonal immunoglobulins (monoclonal components, MC) in 4788 unselected samples from 4173 patients. MC were detected in 514 samples from 390 patients. In 356 these were detected by SPE; the other 34 had a normal SPE pattern but an abnormal kappa:lambda light-chain ratio (KLR). Only 208 of the 356 (58%) samples with bands by SPE had abnormal KLRs. Samples with MC concentrations greater than 5 g/L had a higher proportion of abnormal KLRs (75%) than those with concentrations less than 5 g/L (42%). The KLR was abnormal in 13% of samples in which no MC were visible by SPE or immunofixation electrophoresis (IFE). Compared with quantitative measurements of immunoglobulin heavy and light chains, high-quality SPE remains the method of choice for the detection of MC. Quantitative methods, however, are able to detect additional MC, especially those containing free light chains, and in the absence of SPE and IFE will detect about 75% of MC present at greater than 5 g/L.

Published
1991

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