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Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Studies have confirmed that asiatic acid promotes osteoblast differentiation and inhibits osteoblast apoptosis in a concentration-dependent manner. However, the specific mechanism of asiatic acid on bone biomechanics and Runx2 protein is still unclear. OBJECTIVE: To investigate the effects of asiatic acid on biomechanical characteristics, trabecular area and Runx2 protein expression in osteoporotic rats. METHODS: Forty-eight Sprague-Dawley rats were selected and randomly divided into four groups (n=12 per group): sham operation group, model group, lowdose asiatic acid group, and high-dose asiatic acid group. Except for the sham operation group, rats in the other groups were bilaterally ovariectomized to establish osteoporosis models. The rats in the sham operation and model groups were given 3 mL/(kg·d) normal saline after surgery, while those in the low- and high-dose asiatic acid groups were given 60 and 120 mg/(kg·d) asiatic acid by gavage, respectively. Intervention in each group lasted for 60 days. RT-PCR and western blot were used to detect osteoprotegerin and Runx2 at mRNA and protein levels, respectively. Biomechanical characteristics, trabecular bone area and inflammatory factor levels of rats were compared among groups. RESULTS AND CONCLUSION: (1) The maximum load, stiffness, total energy and elastic modulus of rats in the sham operation group were higher than those in the model group (P < 0.05). Compared with the model group, both high- and low-dose asiatic acid could increase the above-mentioned indexes (P < 0.05). Moreover, the maximum load, stiffness, total energy and elastic modulus were higher in the high-dose asiatic acid group than the low-dose asiatic acid group (P < 0.05). (2) The trabecular bone area was higher in the sham operation group than the model group (P < 0.05). The trabecular bone area was lower in the model group than the low- and high-dose asiatic acid group (P < 0.05). The trabecular bone area was higher in the high-dose asiatic acid group than the lowdose asiatic acid group (P < 0.05). (3) The serum level of transforming growth factor-β1 was significantly lower in the model group than the other three groups (P < 0.05) and significantly higher in the high-dose asiatic acid group than the low-dose asiatic acid group (P < 0.05). The levels of osteocalcin, alkaline phosphatase and tumor necrosis factor-αwere significantly higher in the model group than the other three groups (P < 0.05) and significantly lower in the highdose asiatic acid group than the low-dose group (P < 0.05). (4) The mRNA expression levels of osteoprotegerin and Rnux2 were higher in the sham operation group than the model and low-dose asiatic acid groups (P < 0.05), and the mRNA expression levels of osteoprotegerin and Rnux2 in the high-dose asiatic acid group were higher than those in the sham operation group (P < 0.05). (5) The protein expression levels of osteoprotegerin and Rnux2 were lower in the model group than the other three groups and higher in the high-dose asiatic acid group than the low-dose asiatic acid group. To conclude, asiatic acid can improve the biological characteristics of bone tissue, increase the area of trabecular bone, and reduce the level of inflammation in osteoporotic rats. Its mechanism of action may be related to the promotion of Runx2 expression. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Biological scaffolds made from tissue or organ decellularization have been successfully applied to various tissue engineering and regenerative medicine. OBJECTIVE: To review the preparation methods of acellular scaffolds and their functions and applications in tissue engineering and regenerative medicine. METHODS: Databases of PubMed and CNKI were retrieved with the key words of “decellularization, scaffold, extracellular matrix, tissue regeneration and repair” in English and Chinese. Subjects and abstracts were initially screened to exclude documents that were not related to the subject of the article, and finally 66 articles were included for result analysis. RESULTS AND CONCLUSION: The decellularized scaffolds obtained by decellularizing tissues and organs through physical, chemical and biological methods can effectively retain the components of the extracellular matrix, are non-immunogenic, have good biocompatibility and mechanical properties, and can promote cell adhesion, proliferation, migration and differentiation, induce tissue repair, and reconstruct vascularized and functional tissues and organs. Acellular scaffolds have achieved ideal results in vitro and short-term in vivo experiments of many tissues and organs, such as heart, liver, kidney, lung, trachea, skin and bone, indicating that acellular scaffolds play an important role in tissue regeneration and repair, and have good application prospects. However, to be put into clinical use, more in-depth research and exploration are needed. [ABSTRACT FROM AUTHOR]

OBJECTIVE: Drug therapies have limited effect on neuropathic pain and result in many side effects. Comparatively speaking, exercise is a good way to relieve pain. Here, we systematically evaluate the interventional effect of exercise on neuropathic pain induced by sciatic nerve injury in rats and mice. METHODS: WanFang, CNKI, PubMed, Embase and Web of Science databases were retrieved before April 2020. A series of studies regarding the effect of treadmill, swimming, wheel and other exercises on neuropathic pain induced by sciatic nerve injury in rats and mice were collected. According to the inclusion criteria, two researchers independently completed literature screening, data extraction and conducted methodological quality evaluation using SYRCLE’ s risk of bias tool for animal studies. RevMan 5.3 and STATA 12.0 analysis software were used for statistical analysis. Results: A total of 12 literatures regarding controlled animal studies were included, and there were 133 rodents in the exercise group and 135 rodents in the sedentary group. Meta-analysis showed that exercise could significantly increase paw withdrawal mechanical threshold [standard mean difference (SMD)=0.84, 95% confidence interval (CI): 0.28-1.40, P=0.003] and paw withdrawal thermal latency (SMD=1.54, 95% CI: 0.93-2.15), P < 0.000 01) in rats and mice after sciatic nerve injury. Subgroup analyses were performed for duration of exercise, indicating a significant reduction in heterogeneity. Postoperative exercise duration ≤ 3 weeks could significantly elevate paw withdrawal mechanical threshold (SMD=1.04, 95% CI: 0.62-1.46, P < 0.000 01). Postoperative exercise duration ≤ 4 weeks could significantly improve paw withdrawal thermal latency (SMD=1.93, 95% CI:1.19-2.67, P < 0.000 01). CONCLUSION: Exercise can effectively improve mechanical and thermal hypersensitivity caused by sciatic nerve injury in rats and mice. Pain models, exercise start point, exercise forms, and species are not the main factors of exercise-induced hypoalgesia, while postoperative exercise duration has an important influence on exercise-induced hypoalgesia. [ABSTRACT FROM AUTHOR]

BACKGROUND: Autologous adipose tissue had been became an excellent filling material because of its advantages of no immune rejection, convenient material extraction, and convenient operation. The study found that the transplanted adipocyte had been in the environment of ischemia and hypoxia before the reconstruction of the vascular network, and such an environment was easy to induce apoptosis and necrosis of adipocytes. How to accelerate the process of proliferation and angiogenesis and inhibit the apoptosis of fat cells is the focus of research on improving the survival rate of autologous fat transplantation. OBJECTIVE: To explore the mechanism of the effect of miR-210 on the survival of autologous fat transplantation via YWHAG. METHODS: After 8 weeks of autologous adipose tissue transplantation in Sprague-Dawley rats, the adipose tissue on the back was stripped. The expression levels of miRNAs in adipose tissue were detected by RT-qPCR. The targeting relationship between miR-210 and YWHAG was validated by dual luciferase reporter gene. Adipocytes were isolated from the fat tissues and divided into miR-210 inhibition group, YWHAG overexpression group, miR-210 and YWHAG overexpression group for cell transfection. The expression levels of vascular endothelial growth factor, Endostatin and YWHAG were detected by western blotting. The effects of the miR-210/YWHAG axis on proliferation, apoptosis and angiogenesis of adipocytes were detected by CCK-8, Annexin V-FITC/PI and angiogenesis assay, respectively. RESULTS AND CONCLUSION: (1) miR-210 was highly expressed in adipose tissue (P < 0.001). (2) The results of dual luciferase reporter gene indicated that YWHAG was targeting protein of miR-210 (P < 0.05). (3) Knockdown of miR-210 significantly inhibited proliferation, angiogenesis and induced apoptosis of adipocytes (P < 0.05). (4) Overexpression of miR-210 and YWHAG at the same time had no significant effect on adipocyte proliferation, apoptosis and angiogenesis. (5) In summary, miR-210 promoted autologous fat graft survival by downregulating the expression level of YWHAG to promote proliferation, angiogenesis and inhibiting apoptosis of adipocytes. [ABSTRACT FROM AUTHOR]

BACKGROUND: Autologous peripheral blood hematopoietic stem cell transplantation has been widely used in clinic because of its wide age range, fewer complications and low cost. The reconstitution of hematopoietic function after transplantation mainly depends on the quantity and quality of hematopoietic stem cells. Based on the clinical practice, this paper analyzes various factors affecting the collection of peripheral blood hematopoietic stem cells, and calculates the appropriate blood routine standards. It is of great significance to improve the success rate of peripheral blood hematopoietic stem cell collection and autologous transplantation. OBJECTIVE: To analyze the influence of related factors on the number of CD34+ cells during the mobilization and collection of peripheral blood hematopoietic stem cells of patients with hematological tumors. METHODS: A retrospective analysis of the effects of various factors on the number of CD34+ cells collected during the mobilization and collection of peripheral blood hematopoietic stem cells of 36 patients in Cancer Hospital & Shenzhen Hospital, Chinese Academy of Medical Sciences was performed from June 2018 to June 2020, including age, gender, body surface area, disease type, various cell levels on the day of collection, number of chemotherapy before collection, and disease before collection factors such as status, presence or absence of bone marrow involvement at the first diagnosis. RESULTS AND CONCLUSION: (1) The number of CD34+ cells was related to the number of leukocytes, the absolute value of monocytes, the proportion of monocytes, and the proportion of lymphocytes on the day of collection, and the difference was statistically significant (all P < 0.05). Further analysis of the above four indicators found that the absolute value of monocytes on the day of collection had the highest predictive value for the number of CD34+ cells, followed by the proportion of lymphocytes, leukocytes, and monocytes. (2) Single factor analysis found that age, disease type, mobilization plan, the number of chemotherapy before collection and the presence or absence of bone marrow invasion at the initial diagnosis had no effect on the final collection success rate, and the difference was not statistically significant (all P > 0.05), while gender, disease status before mobilization, body surface area and the final collection success rate were related, and the differences were all statistically significant (all P < 0.05). (3) Results concluded that after mobilization, the blood routine monitoring is used to determine the collection time of peripheral blood hematopoietic stem cells, which mainly monitor the number of leukocytes, the absolute value of monocytes, the proportion of monocytes, and the proportion of lymphocytes. The absolute value of monocytes is the highest predictive value. The cutoff is 1.345×109 /L. When the subject is male, pre-collection disease state is complete remission or partial remission, and body surface area is large, the final collection success rate is high. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Single-segment and double-segment osteotomies are often used to treat ankylotic kyphosis. However, the selection of preoperative strategies, especially for segmental and osteotomy methods, often depends on clinical experience. At present; there are few reports on the biomechanics of double-segment vertebral column decancellation and vertebral column resection osteotomy. OBJECTIVE: To establish a two-segment osteotomy model for ankylotic kyphosis, and to compare and discuss the total displacement of the spine, stress analysis of the internal fixation system, and equivalent stress intensity of the osteotomy contact surface. METHODS: MIMICS software and Geomagic studio software were used to establish two kinds of models of ankylotic kyphosis with vertebral column resection osteotomy and vertebral column decancellation. Each kind of model was divided into single-segment osteotomy and double-segment osteotomy, i.e., L1 single-segment vertebral column resection osteotomy model, L1 single-segment decancellated osteotomy model, L2 single-segment vertebral column resection osteotomy model, L2 single-segment vertebral column decancellation model, T12L2 double-segment vertebral column resection osteotomy model, T12L2 double-segment vertebral column decancellation model, T12L3 double-segment vertebral column resection osteotomy model, and T12L3 double-segment vertebral column decancellation model. ANASYS software was imported to load model. The whole spine displacement, pedicle screw, connecting rod, and bone interface equivalent stress nephogram were recorded under different conditions of osteotomy. RESULTS AND CONCLUSION: (1) Whether it was vertebral column decancellation or vertebral column resection osteotomy model, the total spinal displacement of single-segment osteotomy was less than that of double-segment osteotomy. The displacement of vertebral column resection osteotomy was less than that of vertebral column decancellation in both single- and double-segment osteotomy models. L2 single-segment vertebral column resection osteotomy model had minimal displacement. (2) Whether it was vertebral column decancellation or vertebral column resection osteotomy model, equivalent stress of the single-segment osteotomy was less than that of the double-segment osteotomy. The equivalent stress of the internal fixation device of the vertebral column resection osteotomy was less than that of vertebral column decancellation in both single- and double-segment osteotomy models. The equivalent stress of the internal fixation device of the L1 single-segment vertebral column resection osteotomy was smallest. (3) The equivalent stress of the osteotomy contact surface of all single-segment osteotomy models was smaller than 28 MPa. In the two-segment osteotomy model, the equivalent stress of the osteotomy contact surface of the vertebral column resection osteotomy was less than that of vertebral column decancellation. (4) These results suggest that the biomechanical stability of the single-segment osteotomy model was better than that of the double-segment osteotomy model. The stability of vertebral column resection osteotomy was better than that of vertebral column decancellation. [ABSTRACT FROM AUTHOR]

BACKGROUND: Overuse tendinopalhy is a common disease with no ideal treatment because the pathnogensis of tendinopathy is still unclear. OBJECTIVE: To explore the effects of enzymes effecting collagen synthesis on the metabolism of collagen I and collagen Ill during load accumulation in a model of patella-patellar tendon junction rweruse injury. METHODS: Thirty-two adult female New Zealand wihite rabbits were randomly divided into a jumping group (n=16) and a control group (n=16). In the jump group, rabbits were subjected to jump training for 2, 4, 6, and 8 weeks, 150 jumps per day, 5 days per week. No intervention was performed in the control group. Bilateral patella-patellar tendon junction samples were taken at 24 hours after 2-, 4-, 6-, and 8- week training. The time-series changes of matrix metalloproteinase 1 (MMP1), tissue inhibitor of metelloproteinase 1 (T IMP1), collagen I, and collagen Ill ware measured by immunohistochemistry. The study was performed with an ethic approval from the Animal Ethical Committee of Be jing Sport University, China (approval No. BSU2015022). RESULTS AND CONCLUSION: Compared with the control group, the MMP1 activity was significantly lower within the first 6 weeks of training, TIMP1 activity was significantly higher at 4-8 weeks of training, and TIMP1/MMP1 was significantly higher at 4-8 weeks of training. Compared with the control group, the expression of collagen I was significantly increased in the first 6 weeks of training, but the expression of collagen Ill was significantly lowered after 8 weeks of training. TIMP1 activity was significantly positively correlated with collagen I expression, and MMP1 activity was significantly negatively correlated with collagen I expression. To conclude, the patella-patellar tendon junction, during jumping load accumulation, mainly responded to load stimulation by increasing collagen I expression. In the first 2 weeks of load accumulation, the expression of collagen I was mainly increased by inhibiting MMP1 activity; in the period of 4 to 6 weeks of load accumulation, the expression of collagen I was mainly increased by increasing the activity of TIMP1 and inhibiting the activity of MMP1; and after 8 weeks of load accumulation, TIMP1 could promote collagen synthesis but with inability to antagonize the degradation of collagen I and Ill by MMP1 . Then the expression of collagen I decreased from the peak level after 6 weeks of training to the control group level, and the level of collagen Ill decreased to be less than the level of the control group. [ABSTRACT FROM AUTHOR]

BACKGROUND: Nesfatin-1, a novel feeding regulator, has been found to be closely linked to orthopedic diseases in recent years. The correlation between Nesfatin-1 and osteonecrosis of the femoral head (ONFH) remains unclear. OBJECTIVE: To determine the changes in plasma Nesfatin-1 level in patient with ONFH and to study its correlation with the development of ONFH. METHODS: From May to December 2016, we enrolled 86 patients with ONFH who were hospitalized in the Hip Preserving Ward, No. 3 Orthopedic Department, the First Affiliated Hospital of Guangzhou University of Chinese Medicine (including 13 ARCO stage 11, 26 ARCO stage 111, and 47 ARCO stage IV) and 86 healthy controls. In the ONFH group, there were 26 cases of steroid-induced ONFH, 40 cases of alcoholic ONFH, 12 cases of traumatic ONFH, and 8 cases of idiopathic ONFH. The levels of Nesfatin-1 in peripheral venous blood were measured by enzyme-linked immunosorbent assay (ELISA). Comparison was performed between different ARCO stages, different etiologies as well as before and after collapse. The study protocol was approved by the Ethics Committee of the First Affiliated Hospital of Guangzhou University of Chinese Medicine (No. 20140307), and each patient had signed an informed consent form prior to inclusion in the study. RESULTS AND CONCLUSION: Plasma Nesfatin-1 level was significantly higher in ONFH patients than healthy controls (P=0.000). There were insignificant differences among different etiologies (P=0.956), but significant differences among different ARCO stages (P=0.011) as well as before and after collapse (P=0.003). There was a positive correlation between plasma Nesfatin-1 level and ARCO sieges. Receiver operator characteristic curve analysis showed that Nesfatin-1 level had sensitivity (52.05%) and specificity (92.31 %) in the diagnosis of ONFH. These findings indicate that Nesfatin-1 is closely related to the development of ONFH. It can reflect the degree of necrosis, which can provide reference for clinical diagnosis and trea1ment of ONFH. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Chronic non-specific low back pain seriously affects people’s working and life. Gait trainingdoes help to treat chronic low back pain. Studying the gait of chronic non-specific low back pain patients can help us design more targeted gait re-education content.OBJECTIVE: To find the gait characteristics of young chronic non-specific low back pain patients by comparing the spatio-temporal parameters and mechanical parameters of gait among between healthy youth and youth with chronic non-specific low back pain, so as to provide a reference for the diagnosis and rehabilitation of chronic non-specific low back pain.METHODS: Sixty-five volunteers were enrolled, including 30 healthy youth(control group), and 35 youth with chronic non-specific low back pain(trial group). The gait during normal movement was detected using LORAN 2-m footscan plantar pressure plate and gait testing system.RESULTS AND CONCLUSION:(1) The step length symmetry index, standing phase symmetry index and single-support phase symmetry index in the trial group were significantly higher than those in the control group(all P < 0.01).(2) The spatio-temporal parameters of gait between two sides in the control group showed no significant differences(all P > 0.05). In the trail group, the step length and foot angle deviation of the right side were significantly higher than those of the left side(P < 0.01, P < 0.05). The standing phase and single support time of the right side were significantly longer than those of the left side(all P < 0.01).(3) The vertical ground reaction force symmetry index of initial double-support, single-support, and final double-support in the trial group were all significantly higher than that in the control group(P <0.05, P < 0.01, P < 0.05).(4) In summary, the youth with chronic non-specific low back pain are more likely to have asymmetrical gait than healthy youth. Youth with chronic non-specific low back pain are preferred to support by dominant limbs. In gait re-education for youth with chronic non-specific low back pain, asymmetrical limb support mode should be adjusted. [ABSTRACT FROM AUTHOR]

BACKGROUND: For fracture of distal radius in adults, choosing casting external or open reduction and internal fixation (ORIF) with plates remains controversial. OBJECTIVE: To analyze the results of closed reduction and casting versus ORIF with plates in treatment of fracture of distal radius in adults. METHODS: Databases of PubMed, EMbase, The Cochrane Library (Issue 11, 2017), OVID, Web of Science, CNKI and WanFang were searched. The randomized controlled trials about casting and plate for the fracture of distal radius published before March 2018 were collected. The literature screening was conducted by two researchers independently based on the incision and exclusion criteria, followed by data extraction and bias evaluation. Meta-analysis was performed on RevManager 5.3 software. RESULTS AND CONCLUSION: (1) Fifteen randomized controlled trials were included involving 1 545 patients. (2) Meta-analysis results showed that there were no significant differences in the Disabilities of the Arm, Shoulder and Hand scores (MD=1.83, 95%CI (-0.06, 3.72), P=0.06), Patient-Rated Wrist Evaluation Questionnaire scores (MD=4.01, 95%CI (-4.96, 12.99), P=0.38), range of motion of the wrist joint in dorsiflexion and ulnar deviation (MD=-7.47, 95%CI (-15.40, 0.47), P=0.07; MD=-5.45, 95%CI (-13.82, 2.93), P=0.20) between two groups. The plate groups had a higher Gartland and Werley category (OR=0.52, 95%CI (0.30, 0.90), P=0.02) and grip strength (MD=-10.01, 95%CI (-17.04，-2.99), P=0.005). (3) In the plate group, the incidence of tendinitis (RR=0.10, 95%CI (0.02, 0.51), P=0.006), carpal tunnel syndrome (RR=3.20, 95%CI (1.14, 8.96), P=0.03) and finger stiffness (RR=9.29, 95%CI (1.80, 47.79), P=0.008) were significantly increased. (4) To conclude, for adult patients with fracture of distal, the long-term function between casting and plate is similar. Although the radiological parameters of closed reduction and casting are poor, it does not reduce the range of motion of the wrist joint, nor increase the rate of reoperation, which is firstly chosen for older adults. [ABSTRACT FROM AUTHOR]

BACKGROUND: Induced pluripotent stem cells (iPSCs) hold enormous potential as a tool to generate cells for disease modeling and regenerative medicine. The premise for clinical application of iPSCs is the efficient reprogramming of somatic cells to iPSCs. In recent years, increasing studies have demonstrated that integrins and E-cadherin play important roles in the reprogramming and maintenance of iPSCs. Investigations on the underlying molecular mechanism will help us developing safer and more efficient reprogramming methods for iPSCs generation and deeply understanding the reprogramming process. OBJECTIVE: To summarize the effect of integrins and E-cadherin on the reprogramming of iPSCs and the maintenance of pluripotency based on the insights into the underlying molecular mechanisms. METHODS: Scientific papers published from 2006 to present that described the effects of cell adhesion on reprogramming and stemness maintenance of iPSCs were retrieved by the first author in the Web of Science. Sixty-three articles related to the purpose of this review were reviewed. RESULTS AND CONCLUSION: Integrins-mediated cell-matrix adhesion and E-cadherin-mediated cell-cell adhesion can regulate pluripotent gene expression through a variety of downstream signaling pathways, and can also play important roles in the organization and contractility of actin cytoskeletons that generate gene-, protein-, and epigenetic-level changes and ultimately influence cell reprogramming and stemness maintenance. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: For the treatment of middle clavicle fractures, elastic intramedullary nailing has gradually gained everyone's approval because of its small incision and short operation time. Locking plate fixation is currently the main surgical treatment. At present, there are a few comparative studies on the results of the two surgical treatments, but the results are still controversial. In particular, there is a lack of comparative studies on long-term efficacy and complications. OBJECTIVE: To compare clinical results and complications of locking plate and elastic intramedullary nailing for middle clavicular fracture. METHODS: A retrospective analysis of 85 patients with middle clavicular fractures who were hospitalized at the Army General Hospital of Second Medical College of Southern Medical University from June 2014 to June 2015 undergoing surgeries was performed. They were divided into two groups randomly: locking plate group (n=62) and elastic intramedullary nailing group (n=23). The incision length, operation time, blood loss and time of hospital staying were compared between the two groups. Regular follow-up, radiography assessment and postoperative complications and shoulder function score were measured. RESULTS AND CONCLUSION: (1) Incision length, operation time, blood loss and time of hospital staying were significantly better in the elastic intramedullary nailing group than in the locking plate group (P < 0.05). (2) The complications of the elastic intramedullary nailing group were significantly lower than that of the locking plate group (P < 0.05). The main complications of locking plate group were superficial wound infection, hypesthesia in the local incision and implant protuberance. Only two patients in the intramedullary nailing group experienced complications. (3) The healing rate of elastic intramedullary nailing group was higher than that of the locking plate group, which was not statistically significant (P > 0.05). (4) The DASH scores and the Constant-Murley scores of the two groups after operation were not statistically significant (P > 0.05). (5) The elastic intramedullary nailing and locking plate are both effective methods for the treatment of middle clavicular fracture. Incision length, operation time, blood loss and average length of hospital stay have obvious advantages; the incidence of complications is low; and recovery is fast in the elastic intramedullary nailing group. However, elastic nail may stimulate the local skin, causing some impact for the patients' daily life. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Increasing studies have shown that solid lipid nanoparticles made from traditional Chinese medicine can inhibit cancer cell proliferation and induce cell apoptosis. OBJECTIVE: To investigate the mechanisms of oridonin solid lipid nanoparticles (ORI-SLN) by the regulation of Wnt/β-catenin signaling pathway on the proliferation and apoptosis of esophageal cancer cells. METHODS: After 0, 2.5, 5, 10, 20 μmol/L ORI-SLN treated human esophageal cancer cell lines Eca-109 for 24, 48, 72 hours, the cell inhibition rate was detected by cell counting kit-8, and the half maximal inhibitory concentration (IC50) was calculated. After 0, 14 μmol/L ORI-SLN treated Eca-109 cells for 48 hours, the cell apoptosis was detected by flow cytometry. The expression of Cleaved caspase3, β-catenin, C-myc, Cyclin D1 proteins was detected by western blot assay. Wnt/β-catenin signaling pathway activator LiCl and LiCl+ORI-SLN were used to treat Eca-109 cells for 48 hours, and then the relevant indicators were reexamined. Eca-109 cells without any treatment were used as controls. RESULTS AND CONCLUSION: The cell inhibition rate of Eca-109 cells treated with different concentrations of ORI-SLN for 24, 48 and 72 hours was significantly higher than that at 0 hour, and the cell inhibition rate was found to increase with the prolongation of time and the increase of the concentration (P < 0.01). 14 μmol/L ORI-SLN was confirmed to result in the higher cell apoptosis and Cleaved caspase3 expression compared with the 0 μmol/L group, while the expression of β-catenin, C-myc, Cyclin D1 proteins were significantly lower than the 0 μmol/L group (P < 0.01). Cell inhibition rate, apoptosis rate and Cleaved caspase3 protein expression in the activator group and ORI-SLN+activator group were significantly higher than those in the control group, and the expression of β-catenin, C-myc, Cyclin D1 protein was significantly lower than those in the control group (P < 0.01). The cell inhibition rate, apoptosis rate and expression of Cleaved caspase3 in ORI-SLN+activator group was significantly lower than those in the activator group, and the β-catenin, C-myc, Cyclin D1 protein expression was significantly higher than that in the activator group (P < 0.01). To conclude, ORI-SLNs can inhibit the proliferation and apoptosis of human esophageal carcinoma cell line Eca-109, and its mechanism is related to the regulation of Wnt/β-catenin signaling pathway. [ABSTRACT FROM AUTHOR]

BACKGROUND: With the wide application of bone repair scaffold in the field of medicine, nano-hydroxyapatite (nHA) and silk fibroin (SF) both of which have good biological properties have become research hotspots in recent years. OBJECTIVE: To study the feasibility of nHA/SF composite materials loaded with recombinant human bone morphogenetic protein 2 (rhBMP-2) to restore the initial stability of the spinal segment in rabbits. METHODS: Thirty-six male health New Zealand rabbits were randomly divided into three groups, followed by preparation of spinal instability models. Autogenous iliac bone, nHA/SF composite, and rhBMP-2/nHA/SF composite were implanted into the L4/5 spinal segment in autologous bone group, nHA/SF group and rhBMP-2/nHA/SF group, respectively. X-ray exmaination was performed at 12 weeks postoperatively, and then the animals were killed for gross observation. The stability of the fusion segments was tested through a tensile machine. Histologically, bone graft fusion at the surgical site was observed. RESULTS AND CONCLUSION: (1) Findings from the gross specimen observation showed that the specimens at the fusion site presented with a hard texture. Obvious signs of fusion were visible in the autologous bone group, followed by the rhBMP-2/nHA/SF group, while no signs of fusion were detected in the nHA/SF group. (2) At 12 weeks postoperatively, a large number of trabecular bones grew into the boundary between the vertebral body and the iliac crest graft block in the autologous bone group. A little trabecular bone was found in the boundary in the nHA/SF group, while a lot of trabecular bone tissues were found in the boundary in the rhBMP-2/nHA/SF group. In accordance with the standard of fusion, there were 10, 3, and 9 rats in the autologous bone, nHA/SF and rhBMP-2/nHA/SF groups, respectively. (3) The range of motion of the spine in the rhBMP-2/nHA/SF showed no statistical difference from that in the autologous bone group, but was significantly higher than that in the nHA/SF group (P < 0.05). (4) Osseous connection was found around the bone graft in the autologous bone and nHA/SF groups, but no mature bone tissue was visible in the latter group. In the rhBMP-2/nHA/SF group, a large number of new capillaries was found and permeated into the spinal tissues. In summary, nHA/SF composite materials loaded with rhBMP-2 possess good biocompatibility, mechanical properties and bone induction ability, which can rebuild the initial stability of the spine in a short time. [ABSTRACT FROM AUTHOR]