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Using unmodified Au nanorods with enzyme-linkage reaction, a label-free colorimetric method for simple and convenient assay of DNA methylation is presented. [ABSTRACT FROM AUTHOR]

An imidazolyl hydrogen-bonded organic framework (HOF-T) with outstanding thermal and water stability was constructed by C–H⋯N hydrogen bonding and C–H⋯π interactions. UO22+ can be selectively captured by the imidazole group of HOF-T and rapidly reduced to UO2 under visible light irradiation, realizing exceptional uranium removal with high capacity and fast kinetics. [ABSTRACT FROM AUTHOR]

Herein, a novel fluorescent ionic covalent organic framework (BTTA–BDNP) based on a linked carbazole unit was constructed for the synchronous monitoring and capture of TcO4−/ReO4−. BTTA–BDNP has a fast fluorescence response time with a low detection limit (66.7 nM) for ReO4− (a non-radioactive substitute for TcO4−). Meanwhile, the high charge density and hydrophobic skeleton of BTTA–BDNP enable it to exhibit rapid and selective trapping of ReO4− in complex environments. [ABSTRACT FROM AUTHOR]

We demonstrate for the first time the utilization of fluorogenic RNA aptamers for label-free uracil-DNA glycosylase (UDG) assay. Through rationally engineering the transcription machine with dU substitution, this assay requires only a single probe to simultaneously sense and amplify the UDG signal, achieving a low detection limit of 6.3 × 10−6 U mL−1. Moreover, it can be applied for screening UDG inhibitors and measuring endogenous UDG activity in different cells. [ABSTRACT FROM AUTHOR]

We develop for the first time a label-free fluorescent method for sensitive detection of fat mass and obesity-associated protein (FTO) activity using MazF-mediated primer generation rolling circle amplification. This method is very simple with ultrahigh sensitivity and good specificity, and it can detect FTO activity at the single-cell level. Moreover, this method can be applied for the measurement of kinetic parameters and the screening of FTO inhibitors. [ABSTRACT FROM AUTHOR]

We demonstrate the zirconium ion-mediated assembly of a single quantum dot (QD)-based nanosensor for accurate detection of protein kinases (PKA) and polynucleotide kinases (PNK). This nanosensor is very sensitive with a detection limit of 8.82 × 10−4 U mL−1 for PKA and 1.40 × 10−5 U mL−1 for PNK. Moreover, it can be used to analyze the enzyme kinetic parameters and screen the inhibitors of PKA and PNK, with potential applications in drug discovery and clinical diagnosis. [ABSTRACT FROM AUTHOR]

We developed a single quantum dot-based fluorescence resonance energy transfer biosensor for antibody-free detection of ten-eleven translocation 1 (TET1). This biosensor can sensitively detect TET1 in a homogeneous manner without the involvement of any specific antibodies, and it can be used for accurate measurement of TET1 activity in human neuroblastoma cells and the screening of TET1 inhibitors. [ABSTRACT FROM AUTHOR]

We demonstrate the development of a new fluorescent biosensor for sensitive DNA methylation assay by integrating single-molecule detection with endo IV-assisted signal amplification. This biosensor possesses the characteristics of good selectivity and high sensitivity with a detection limit of 7.3 × 10−17 M. It can distinguish as low as 0.01% methylation level, and can analyze genomic DNA methylation even in a single cancer cell. [ABSTRACT FROM AUTHOR]

In the present work, we have introduced a series of stable radical-doped coordination compounds composed of donor–acceptor structures and shown to produce organic radicals in situ as a result of unconventional lone pair–π interactions in ambient conditions. Inconspicuous lone pair–π and C–H⋯π interactions were shown to play a key role in self-assembly as well as the charge transfer process, resulting in a long-lived charge-separated state able to generate organic radicals. The resultant species displayed broad-spectrum antimicrobial activity, including against multi-drug-resistant bacteria. This study unveiled the promise of reactive organic radical-doped materials as a new platform for developing antimicrobial agents that can overcome antibiotic resistance. [ABSTRACT FROM AUTHOR]

We demonstrate for the first time the integration of a dumbbell probe with dual signal amplification for the simultaneous detection of multiple DNA methyltransferases (MTases). This method is very sensitive with a detection limit of 2.15 × 10−5 U mL−1 for DNA adenine methyltransferase (Dam) and 3.23 × 10−6 U mL−1 for CpG Methyltransferase (M.SssI), and it can be used to screen the enzyme inhibitors and simultaneously measure Dam and M.SssI in complex biological samples. [ABSTRACT FROM AUTHOR]

We develop a new fluorescence method for the sensitive detection of protein tyrosine phosphatase 1B (PTP1B) based on dephosphorylation-directed tricyclic DNA amplification cascades. This method exhibits good specificity and high sensitivity with a detection limit of 0.24 pM. Moreover, it can be applied for kinetics analysis, inhibitor screening, and the accurate detection of PTP1B in a variety of cancer cells. [ABSTRACT FROM AUTHOR]

Gold nanoparticles (AuNPs) decorated carbon nitride nanosheets (Au–g-C3N4 NSs) could work as an excellent on-electrode cathodic coreactant for Ru(bpy)32+. Based on the regulation effect of AuNPs and mercury (Hg2+) for the dual-potential electrochemiluminescence (ECL) of Ru(bpy)32+, a simple, sensitive and selective ECL system was created for the ratiometric sensing of Hg2+. [ABSTRACT FROM AUTHOR]

In situ-derived boron-doped graphene quantum dots can significantly improve the activity of boron carbide nanosheets for artificial N2 fixation and reduction with superior electrocatalytic activity (NH3 yield: 28.6 μg h−1 mgcat.−1 at −0.45 V vs. RHE; faradaic efficiency: 16.7%), high electrochemical stability, and high selectivity in 0.1 M HCl under ambient conditions. [ABSTRACT FROM AUTHOR]

A novel ratiometric electrochemiluminescence (ECL) indicator has been constructed for ultratrace As(iii) detection based on the synergistic quenching of ECL emission of Au–g-C3N4 NSs using As(iii) and Ru(bpy)32+, meanwhile generating a new ECL signal of Ru(bpy)32+ with an increased intensity. Due to the dual quenching effect of As(iii) and Ru(bpy)32+ coupled with the generation of the second ECL signal of Ru(bpy)32+, the sensitivity and selectivity for detecting As(iii) are vastly enhanced. [ABSTRACT FROM AUTHOR]

A novel dual-emission fluorescence ratiometric probe of luminol–Tb–GMP CPNPs for highly sensitive and selective detection of ALP and As(v) has been constructed based on the stimulus responsivity of luminol. The introduction of luminol as a ligand for Tb3+, combined with GMP, leads to a sensor which is more robust, sensitive, and efficient. [ABSTRACT FROM AUTHOR]

A novel Au NP mediated dual-potential ECL ratiometric approach for highly sensitive protein kinase activity and inhibition assay has been developed based on the simultaneous decrease of cathodic ECL from GQDs and enhancement of anodic ECL from luminol in the same bioanalysis. [ABSTRACT FROM AUTHOR]

This assay, termed branched cascade enzymatic amplification (BCEA), can be a novel and straightforward method for sensitive and specific microRNA detection in crude cellular extracts of cancer cells at physiological temperature, by coupling two ordinary polymerases, Klenow fragment exo− and terminal deoxynucleotidyl transferase. [ABSTRACT FROM AUTHOR]

A green method was employed for synthesizing peptide-templated nanoclusters without requiring strong reducing agents. Using synthetic peptide–gold nanoclusters as fluorescence probes, a novel assay for detecting protein kinase is developed based on phosphorylation against carboxypeptidase Y digestion. [ABSTRACT FROM AUTHOR]

Using graphene quantum dots (GQDs) with a boronic acid-substituted bipyridinium salt (BBV), a label-free fluorescence assay for glucose detection is presented. [ABSTRACT FROM AUTHOR]