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Sensitive and homogeneous microRNA detection using branched cascade enzymatic amplification.
- Source :
-
Chemical Communications . 7/4/2015, Vol. 51 Issue 52, p10543-10546. 4p. - Publication Year :
- 2015
-
Abstract
- This assay, termed branched cascade enzymatic amplification (BCEA), can be a novel and straightforward method for sensitive and specific microRNA detection in crude cellular extracts of cancer cells at physiological temperature, by coupling two ordinary polymerases, Klenow fragment exo− and terminal deoxynucleotidyl transferase. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ENZYME analysis
*MICRORNA
*CANCER cells
*POLYMERASES
*NUCLEOTIDES
Subjects
Details
- Language :
- English
- ISSN :
- 13597345
- Volume :
- 51
- Issue :
- 52
- Database :
- Academic Search Index
- Journal :
- Chemical Communications
- Publication Type :
- Academic Journal
- Accession number :
- 115165654
- Full Text :
- https://doi.org/10.1039/c5cc02864g