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A dumbbell probe-based dual signal amplification strategy for sensitive detection of multiple DNA methyltransferases.
- Source :
-
Chemical Communications . 11/14/2020, Vol. 56 Issue 88, p13627-13630. 4p. - Publication Year :
- 2020
-
Abstract
- We demonstrate for the first time the integration of a dumbbell probe with dual signal amplification for the simultaneous detection of multiple DNA methyltransferases (MTases). This method is very sensitive with a detection limit of 2.15 × 10−5 U mL−1 for DNA adenine methyltransferase (Dam) and 3.23 × 10−6 U mL−1 for CpG Methyltransferase (M.SssI), and it can be used to screen the enzyme inhibitors and simultaneously measure Dam and M.SssI in complex biological samples. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13597345
- Volume :
- 56
- Issue :
- 88
- Database :
- Academic Search Index
- Journal :
- Chemical Communications
- Publication Type :
- Academic Journal
- Accession number :
- 146846878
- Full Text :
- https://doi.org/10.1039/d0cc05991a